LOCUS       DAYYAJ010000001        79049 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000001 DAYYAJ010000000
VERSION     DAYYAJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.001"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..731
                     /locus_tag="ACHATZ_000001"
     CDS             75..731
                     /locus_tag="ACHATZ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8402567.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(805..1158)
                     /locus_tag="ACHATZ_000002"
     CDS             complement(805..1158)
                     /locus_tag="ACHATZ_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8402568.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            1350..2279
                     /locus_tag="ACHATZ_000003"
     CDS             1350..2279
                     /locus_tag="ACHATZ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8402569.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2290..3126
                     /locus_tag="ACHATZ_000004"
     CDS             2290..3126
                     /locus_tag="ACHATZ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8402570.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            3374..4150
                     /locus_tag="ACHATZ_000005"
     CDS             3374..4150
                     /locus_tag="ACHATZ_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8402571.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            4326..5069
                     /locus_tag="ACHATZ_000006"
     CDS             4326..5069
                     /locus_tag="ACHATZ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402572.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            5062..6642
                     /locus_tag="ACHATZ_000007"
     CDS             5062..6642
                     /locus_tag="ACHATZ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8402573.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(6893..7372)
                     /locus_tag="ACHATZ_000008"
     CDS             complement(6893..7372)
                     /locus_tag="ACHATZ_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8402574.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(7388..8140)
                     /locus_tag="ACHATZ_000009"
     CDS             complement(7388..8140)
                     /locus_tag="ACHATZ_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8402575.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8327
                     /locus_tag="ACHATZ_000010"
     tRNA            8257..8327
                     /locus_tag="ACHATZ_000010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8289..8291,aa:Gly,seq:ccc)
     gene            8688..9413
                     /locus_tag="ACHATZ_000011"
     CDS             8688..9413
                     /locus_tag="ACHATZ_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8402576.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            9668..9934
                     /locus_tag="ACHATZ_000012"
     CDS             9668..9934
                     /locus_tag="ACHATZ_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8402577.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            9931..10362
                     /gene="ruvX"
                     /locus_tag="ACHATZ_000013"
     CDS             9931..10362
                     /gene="ruvX"
                     /locus_tag="ACHATZ_000013"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8402578.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            10387..10692
                     /locus_tag="ACHATZ_000014"
     CDS             10387..10692
                     /locus_tag="ACHATZ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8402579.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(10773..11918)
                     /locus_tag="ACHATZ_000015"
     CDS             complement(10773..11918)
                     /locus_tag="ACHATZ_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8402580.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(11979..12626)
                     /locus_tag="ACHATZ_000016"
     CDS             complement(11979..12626)
                     /locus_tag="ACHATZ_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8402581.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            12820..13113
                     /locus_tag="ACHATZ_000017"
     CDS             12820..13113
                     /locus_tag="ACHATZ_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402582.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13157..13468
                     /locus_tag="ACHATZ_000018"
     CDS             13157..13468
                     /locus_tag="ACHATZ_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402583.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            13465..14089
                     /locus_tag="ACHATZ_000019"
                     /pseudo
     CDS             13465..14089
                     /locus_tag="ACHATZ_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            14230..14388
                     /locus_tag="ACHATZ_000020"
     CDS             14230..14388
                     /locus_tag="ACHATZ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402584.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            14389..14751
                     /locus_tag="ACHATZ_000021"
     CDS             14389..14751
                     /locus_tag="ACHATZ_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402585.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            14788..15636
                     /locus_tag="ACHATZ_000022"
     CDS             14788..15636
                     /locus_tag="ACHATZ_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8402586.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            15626..17101
                     /locus_tag="ACHATZ_000023"
     CDS             15626..17101
                     /locus_tag="ACHATZ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8402587.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            17367..17774
                     /locus_tag="ACHATZ_000024"
     CDS             17367..17774
                     /locus_tag="ACHATZ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8402588.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            17777..17992
                     /locus_tag="ACHATZ_000025"
     CDS             17777..17992
                     /locus_tag="ACHATZ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402589.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            17996..18376
                     /locus_tag="ACHATZ_000026"
     CDS             17996..18376
                     /locus_tag="ACHATZ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8402590.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            18510..18665
                     /locus_tag="ACHATZ_000027"
     CDS             18510..18665
                     /locus_tag="ACHATZ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8402591.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            18734..19207
                     /locus_tag="ACHATZ_000028"
     CDS             18734..19207
                     /locus_tag="ACHATZ_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8402592.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            19204..20898
                     /locus_tag="ACHATZ_000029"
     CDS             19204..20898
                     /locus_tag="ACHATZ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8402593.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            20864..21049
                     /locus_tag="ACHATZ_000030"
     CDS             20864..21049
                     /locus_tag="ACHATZ_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402594.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            21053..22228
                     /locus_tag="ACHATZ_000031"
     CDS             21053..22228
                     /locus_tag="ACHATZ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8402595.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            22221..23744
                     /locus_tag="ACHATZ_000032"
     CDS             22221..23744
                     /locus_tag="ACHATZ_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8402596.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            23800..24084
                     /locus_tag="ACHATZ_000033"
     CDS             23800..24084
                     /locus_tag="ACHATZ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8402597.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            24071..24406
                     /locus_tag="ACHATZ_000034"
     CDS             24071..24406
                     /locus_tag="ACHATZ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8402598.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            24701..25327
                     /locus_tag="ACHATZ_000035"
     CDS             24701..25327
                     /locus_tag="ACHATZ_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402599.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            26069..26905
                     /locus_tag="ACHATZ_000036"
     CDS             26069..26905
                     /locus_tag="ACHATZ_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8402600.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            26969..27400
                     /locus_tag="ACHATZ_000037"
     CDS             26969..27400
                     /locus_tag="ACHATZ_000037"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8402601.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(27462..28784)
                     /locus_tag="ACHATZ_000038"
     CDS             complement(27462..28784)
                     /locus_tag="ACHATZ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8402602.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(28938..30578)
                     /locus_tag="ACHATZ_000039"
     CDS             complement(28938..30578)
                     /locus_tag="ACHATZ_000039"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8402603.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            30911..31381
                     /locus_tag="ACHATZ_000040"
     CDS             30911..31381
                     /locus_tag="ACHATZ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8402604.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            31603..32706
                     /locus_tag="ACHATZ_000041"
     CDS             31603..32706
                     /locus_tag="ACHATZ_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8402605.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            32990..33670
                     /locus_tag="ACHATZ_000042"
     CDS             32990..33670
                     /locus_tag="ACHATZ_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8402606.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            33760..34794
                     /locus_tag="ACHATZ_000043"
     CDS             33760..34794
                     /locus_tag="ACHATZ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8402607.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            34808..35143
                     /locus_tag="ACHATZ_000044"
     CDS             34808..35143
                     /locus_tag="ACHATZ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402608.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            35127..37607
                     /locus_tag="ACHATZ_000045"
     CDS             35127..37607
                     /locus_tag="ACHATZ_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402609.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            37663..39222
                     /locus_tag="ACHATZ_000046"
     CDS             37663..39222
                     /locus_tag="ACHATZ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8402610.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            39219..40583
                     /locus_tag="ACHATZ_000047"
     CDS             39219..40583
                     /locus_tag="ACHATZ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8402611.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            40754..41824
                     /locus_tag="ACHATZ_000048"
     CDS             40754..41824
                     /locus_tag="ACHATZ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402612.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            41839..42507
                     /locus_tag="ACHATZ_000049"
     CDS             41839..42507
                     /locus_tag="ACHATZ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402613.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            42527..43105
                     /locus_tag="ACHATZ_000050"
     CDS             42527..43105
                     /locus_tag="ACHATZ_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8402614.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            43506..44417
                     /locus_tag="ACHATZ_000051"
     CDS             43506..44417
                     /locus_tag="ACHATZ_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8402615.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(44795..45712)
                     /gene="rnhC"
                     /locus_tag="ACHATZ_000052"
     CDS             complement(44795..45712)
                     /gene="rnhC"
                     /locus_tag="ACHATZ_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8402616.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            45914..46351
                     /gene="zapA"
                     /locus_tag="ACHATZ_000053"
     CDS             45914..46351
                     /gene="zapA"
                     /locus_tag="ACHATZ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8402617.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            46409..46957
                     /locus_tag="ACHATZ_000054"
     CDS             46409..46957
                     /locus_tag="ACHATZ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8402618.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            47072..49432
                     /locus_tag="ACHATZ_000055"
     CDS             47072..49432
                     /locus_tag="ACHATZ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8402619.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            49519..49833
                     /gene="trxA"
                     /locus_tag="ACHATZ_000056"
     CDS             49519..49833
                     /gene="trxA"
                     /locus_tag="ACHATZ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8402620.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            50015..51793
                     /gene="uvrC"
                     /locus_tag="ACHATZ_000057"
     CDS             50015..51793
                     /gene="uvrC"
                     /locus_tag="ACHATZ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8402621.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            51938..52837
                     /locus_tag="ACHATZ_000058"
     CDS             51938..52837
                     /locus_tag="ACHATZ_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402622.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            52830..54092
                     /locus_tag="ACHATZ_000059"
     CDS             52830..54092
                     /locus_tag="ACHATZ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402623.1"
                     /translation="MRKFWIITKDIYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            54253..56151
                     /locus_tag="ACHATZ_000060"
     CDS             54253..56151
                     /locus_tag="ACHATZ_000060"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8402624.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(56481..57200)
                     /locus_tag="ACHATZ_000061"
     CDS             complement(56481..57200)
                     /locus_tag="ACHATZ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8402625.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    57390..57437
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            57537..57869
                     /locus_tag="ACHATZ_000062"
     CDS             57537..57869
                     /locus_tag="ACHATZ_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402626.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            57856..59859
                     /locus_tag="ACHATZ_000063"
     CDS             57856..59859
                     /locus_tag="ACHATZ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8402627.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            59878..60348
                     /locus_tag="ACHATZ_000064"
     CDS             59878..60348
                     /locus_tag="ACHATZ_000064"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8402628.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            60369..60953
                     /locus_tag="ACHATZ_000065"
     CDS             60369..60953
                     /locus_tag="ACHATZ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8402629.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            60968..61954
                     /locus_tag="ACHATZ_000066"
     CDS             60968..61954
                     /locus_tag="ACHATZ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8402630.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            61944..62255
                     /locus_tag="ACHATZ_000067"
     CDS             61944..62255
                     /locus_tag="ACHATZ_000067"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8402631.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            62277..64058
                     /locus_tag="ACHATZ_000068"
     CDS             62277..64058
                     /locus_tag="ACHATZ_000068"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8402632.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            64051..65427
                     /locus_tag="ACHATZ_000069"
     CDS             64051..65427
                     /locus_tag="ACHATZ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8402633.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            65434..66069
                     /locus_tag="ACHATZ_000070"
     CDS             65434..66069
                     /locus_tag="ACHATZ_000070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8402634.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            66099..67454
                     /locus_tag="ACHATZ_000071"
     CDS             66099..67454
                     /locus_tag="ACHATZ_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8402635.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(67646..69040)
                     /locus_tag="ACHATZ_000072"
     CDS             complement(67646..69040)
                     /locus_tag="ACHATZ_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8402636.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            69132..70103
                     /locus_tag="ACHATZ_000073"
     CDS             69132..70103
                     /locus_tag="ACHATZ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8402637.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            70100..70882
                     /locus_tag="ACHATZ_000074"
     CDS             70100..70882
                     /locus_tag="ACHATZ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8402638.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(71051..71422)
                     /locus_tag="ACHATZ_000075"
     CDS             complement(71051..71422)
                     /locus_tag="ACHATZ_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8402639.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            71556..71999
                     /locus_tag="ACHATZ_000076"
     CDS             71556..71999
                     /locus_tag="ACHATZ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8402640.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(72042..72851)
                     /gene="yidA"
                     /locus_tag="ACHATZ_000077"
     CDS             complement(72042..72851)
                     /gene="yidA"
                     /locus_tag="ACHATZ_000077"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8402641.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(72870..74237)
                     /locus_tag="ACHATZ_000078"
     CDS             complement(72870..74237)
                     /locus_tag="ACHATZ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8402642.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(74221..75051)
                     /locus_tag="ACHATZ_000079"
     CDS             complement(74221..75051)
                     /locus_tag="ACHATZ_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8402643.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            75214..75651
                     /locus_tag="ACHATZ_000080"
     CDS             75214..75651
                     /locus_tag="ACHATZ_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8402644.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            75727..76347
                     /gene="rpoE"
                     /locus_tag="ACHATZ_000081"
     CDS             75727..76347
                     /gene="rpoE"
                     /locus_tag="ACHATZ_000081"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8402645.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            76536..77042
                     /locus_tag="ACHATZ_000082"
     CDS             76536..77042
                     /locus_tag="ACHATZ_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8402646.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            77059..77958
                     /locus_tag="ACHATZ_000083"
     CDS             77059..77958
                     /locus_tag="ACHATZ_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8402647.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            78045..78929
                     /locus_tag="ACHATZ_000084"
     CDS             78045..78929
                     /locus_tag="ACHATZ_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8402648.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        26190 a        13456 c        16433 g        22970 t
ORIGIN      
        1 ttgatttttt cctataaata ctgctatgat ggagaaatca aagatgtaat tagacaatac
       61 gtggaggtca tttcgtgaaa gatcatatta ttccaaaggc gacagctaaa agactgcctt
      121 tgtattatcg ctatttaagg attttaaata atgctggaaa gaaaaaagtg tcttcaactg
      181 aattaagtga agccgttcaa gtagatagtg caacgatccg tcgagatttt tcttattttg
      241 gcgaattagg aaaacgtggg tatggttatg atgtagaaga tcttatgcac ttttttgcaa
      301 agacattaaa cgacgatcaa ttgacaaatg ttgcgctgat cggtgtaggg aacttgggaa
      361 gtgctttact aaaatacaaa ttccatcaaa gtaatagcat acgtatcagt tgtgcttttg
      421 atgtgaatga ggaaatcgtt gggcgcatcg tagacggtat ccccgtttat ccaatggaag
      481 atatgtcaga acaaatcaag atccaacaaa tcgaagtagc aattttaacg atacccgctg
      541 aaagagcaca agaagtagtc aatcagttaa cggaagcagg aatcaaagga atcttgaact
      601 ttaccgctgc tcgattgaat gctccgtcag atgtattgat ccaaaatgtt gatttgacga
      661 atgaattgca aaccttgatc tatttcttgc attctgacgc aaatgcgcat tccgaaaata
      721 cagcagaata aaaataaaaa gcgattgaac aaatgaaggc aaaactttca cttgttcaat
      781 cgcttttttc tgtttggaca acgattattt tgttcggaag atttcttcat aagaaaggga
      841 atcatcttct gctgtcagtt catctagtag ataacggatt tttttaaagt cgatgaaccg
      901 tgcttgccga aagacttctt ttccttgatg aaaaatcagg acaactggcg cagtcagcac
      961 ttcaaattta ctagctactt ctggtgtttc aaatgcatcc aaatgaagtg ccggaatatc
     1021 ataatgtgtg agaagttcct ctagtcgtgg acgaacagcg tgacaaacac tgcaattagg
     1081 catcgaaaga taaatgacgg caacggatga ctgttgcagt atgtcttgaa cttcagtcaa
     1141 gttagtggtt tgtttcattt ttattctccc ttttatgata gaagtgatat gtatgagacc
     1201 ttaatttaat gggaataaca gaaaaagtca attgaagtgc tttggtaaaa aataagagtt
     1261 gtgtaaaaat ttacagagca acttgttttt tttgaataaa agcgtatatt tgcaaagcga
     1321 atgaactaga aataaaaagg aggaaactca tgttttcttt tattccaaat tcggttttgc
     1381 agatgagtac gattttctta tcgatcgtta ttgaagcatt acctttcgta ttattaggct
     1441 gtcttatctc aggcgcctta caagtatttt tgaccccaga acgtgtgaag agatggcttc
     1501 ctgaaaatcg tatcttctct atttttactg gttcgatctt aggatttttc tttccttcat
     1561 gtgaatgcgg catcgtaccg atcgttcatc aatttgttaa aaaaggcgta ccagttcaca
     1621 cagcgtttgc ttttatgctg actgctccga tcatcaatcc aatcgtgatt ttttccactt
     1681 ttatcgcatt tggcaattct tggcagatgg caagctggcg ggtgataggc agcttttttg
     1741 ttgcccttgt gattggtgtc tggcttgcct attttcaaaa agacgctgta ttgaaagaaa
     1801 aaatcacgtc taaagtagca gaaaatcacc atcatcatgt ccatgaagaa gaacaaagtg
     1861 agaagaatga accaaaaaat cagctgatcc atcaaactgg gcatgtgttg acacattcaa
     1921 tcgacgaatt ttttgataca ggtcgttatt tgatcattgg cggattaatc gcagctgcga
     1981 tgcagactta tttaccaact agaattatgc taacgctcgg ctcaacaaag attttagcca
     2041 ttatcatcat gctgttattg gcctttacga tgtctctttg ctcagaagca gatgcgttta
     2101 taggatcttc tttgttaagt ctgttcggca cagcacctgt agctgccttt ttggtctttg
     2161 gcccaatggt cgatatcaaa aatctactga tgatgaaacg atattttaca ggacgcttta
     2221 ttttatcgct gatcggactg attgccctta gcgttattgg attcacatta tttatttagg
     2281 agggaaaata tgctgcggtt tttgatttta tctgggtatt tcgaattgat gatgtacttg
     2341 caagtttctg gcaaactcga tcaatatatc aatgtccatt atcgctactt agctattctt
     2401 tcaatgattt tatctgctct tttggcttta gttcagctgt atttgtgggt caaaaacgga
     2461 aaagcagaag aaaaggcaca cgagcacgat catgaccatg ggttgtcaaa accttatcaa
     2521 cgtggaatcg cttatgtact gctagctttg ccagtaatcg tggggacgtt gtttccaaca
     2581 gttagtctcg acactacgat cgtagaagcc aaaggattta atttcccagt aagtaaagaa
     2641 tcagtagggg atccggagat gcagacacaa tacttaaagc cggataccag tatttatttc
     2701 aacaaatcag actacttaga ccaaatgaat gagttgaaag aaaagtatgg ggataagcaa
     2761 actatcaaga tcacagatga aaactattta gaagttatgg agctaattta taattaccct
     2821 agcgaattca ttggcaaaaa aatcagctat gaaggatttg tttaccaaac accgaacgat
     2881 agcaatacag atatcttctt gttccgattc ggcattatcc attgtgtggc agattcagga
     2941 gtctttggtt tgttgacgca tatgccggaa ggagtaacgg ttaaaaatga tgaatggtat
     3001 aaaatagaag gaaccatcca atcagactat tatcaaccat tcaagcgtga aatcccatct
     3061 gtagttgtta caaacgctga gaaagtctct gctccgaaaa atcagtatgt gtatcgttct
     3121 ttttaaaaga acaaaataaa taaacatagc tcagacagct gacacgcaag gacgtcggtt
     3181 gtctggcttt ttttgcatga tgtattcatt tgtttgatat ttattttaga agcagcatcc
     3241 tgaaagtatt tgatatcggt ttcaaaggtg ttggaaaaga ttgtgaagag aattacaatt
     3301 tattcctaac atgtatgttg aaaagttgta tgaaatgatt ttgatagtaa aatatcgagt
     3361 ggaggtactt tttatggtaa cgcgtatatt tattttggat gatagtttgg tttttgaaaa
     3421 aatgctagta gaaattttgg aagaatccag aattttatta agtccttgga aatttgagat
     3481 cgttcaggga ttgaacgtta tacaatttgt tgaattcatt aaaaataatg aaatctattc
     3541 ctcagatatt tttttcctag acatcgattt gaacgcctat tatagcggta tcgaactagg
     3601 cgaaatgctt agagaaaaga atgaaaagtg taaaattgtt tatctaacgt ctcatccgaa
     3661 taaagcaata actgttatta atcgaaagat tacgcctagt ggttatttga taaaagaatt
     3721 cacttatgaa aaaaataaaa aaagcatcca aagttactta ttgaaagaaa aagaaaacta
     3781 tcctgttcaa catgctacga aaaaaaggtg gacaactgtc cgttatggta atagcgatca
     3841 ctatatccat tacgatgaaa tcctttatct aatgagcatg ccaggttata aaaataaaat
     3901 aggcctagta ttgaaagatg aagagattct cgtaaacggc acaattaaaa aatataaaaa
     3961 atcactcgaa tatgagtatt tatgtaaaga tttaaaagct tatatcatta atactgaaaa
     4021 cattacatct attagcagag taaacgaaat agtaacattt gcaaatggag aacaactaga
     4081 gatgggaaag agaagtattg ataaactaaa gaattttatg caagaaaaga tagaaaatga
     4141 atcattttaa agaaaacaaa gactcatatc ttacctgtta gaagagtgtt ttcctgtaga
     4201 agagaacaac ccaagtagca gcctagtcgt ttctatcctt ttttgttatg cactcttttt
     4261 gacatttact ccaccaataa cgaagggtac aataaaaagt aataacaaac aaaaggagta
     4321 cagaaatgat tgaattcatc aatgtaagta aaagttacgg gaaaaaacaa gcattaaaag
     4381 acttgtctct ctcgataaga caaggggaaa tttttggatt tttaggacat aatggtgccg
     4441 gaaaatcaac tacgattaaa agtttagtta gtattattga accatcatcg ggaacgattt
     4501 tagtagatgg aatgaaattg acggaaaatc gattgagtat caaacaaaaa atcggttatg
     4561 tgcctgattc cccagatatt tttttacaat tgacggctgg agaatattgg gacttgattt
     4621 cagctgccta tgaattagac acacaaaaaa aagaaaagcg tttagcagaa ttgacagcgc
     4681 tttttgatat gtatagtcat caaaatgaaa caatcgcaag tttttcacat ggaatgcgtc
     4741 aaaaaacgat tttgatcggc acgttgctgc cagatccaga tatttgggta ttggatgaac
     4801 ctttgcaagg acttgatcct caagcagcat ttgatttgaa agaaatgatg aaggctcatg
     4861 ccgcaaaagg aaaaacagtc attttttcta cacatgtatt ggataccgca caacagttat
     4921 gcgataaact ggctattttg aagaaaggtg aattgattta tcagggggca gttagtgaat
     4981 tattgaatgg ctcacctgat gaaacgctag aaaagattta tttgaaaatg gctggaaggc
     5041 aagcgacagg agatggaccg tatgtataag cgacaattat tagaattaat tcgagttaat
     5101 cttttatatg ttaatccaca gctcacgaat aaagcaagag agaaaggaaa aaagggaaag
     5161 cgattaacta tctatctttt gtctcaaaat cttttttcag ggctgatttt tttactgatt
     5221 tacggatttg ctatgtttgc tatgaacttt gttaaattgc caggtttctt tacttattac
     5281 gtggcacttt ttggtatgtt aggtttaact caaagtatat cagtcattta taatatcttt
     5341 tttgaaggaa atgacctgca agtatttttg cctctgcctt ttggacaaga acaggttttt
     5401 tcagcaaaga ttttggtagt tgctttgacc atcattcctt ttgtttttcc gcttctagtc
     5461 atgtttattt taaccggatg gcgagctgat ctagggattt tgcttactat cattatctca
     5521 atccttttgt tttctctatt tttgattatt cttttttgtc tggctagttt gattgttttt
     5581 ggtttggctc gaacagcagt gtttaaaaaa catagaaaaa cagtttctag tatcttgctt
     5641 ggcagctcga tgctgatcgc atttgctgga atcatgtgga tgaatcatca aacggggact
     5701 attgaaaatg agttaacaga tcggcatccg atttcgatat tattgccatt ctatgatgct
     5761 gccagtcagc cactttctca acaaggttta ttgggttggg gaatactagc tatcacggct
     5821 ctgggatttt tccttgctgt aaaacagctg atagtaccaa agcttacaga gcaactgact
     5881 gattcaaaca gtgagttcaa aataaaaaga acccataaaa agaaccaaaa tgtgcatcag
     5941 cttttattta gttacaatgc tcaattagta aaagaaccta atttgattat gcaagttctt
     6001 tcttcttcct tgttaactcc aatcattttt attcttgctt ttgccctagg aggcgaaata
     6061 aaattaactg atttagataa taagttaatc ggtgttgttt tcctggtagg gatcgcattg
     6121 gctttcttaa cagttaatca gacttctttt atcagcaatc ttatttcttt ggatcaagaa
     6181 aattttctgt ttatccgttc tttgccaatc tctatgaacc aatatttaaa agaaaagttc
     6241 agattcggat ggatattgca gagtttttta actggtggga tcgcattatt aagcggactg
     6301 tcttttcagc tgcctttgtt ttttatccct agcttgctat ttggttcttt attcggctgc
     6361 tacttattgt cactacgtta tttcgctaga gattatcggt tgctgctgct gaactggacc
     6421 aatattaacc aattatttaa cagaggttca ggaagtttgg gtttagtcgc aactatgatg
     6481 ggttcattga tcatcagtat tattcttatc gtcttatacg gaatgttggc tttgttttat
     6541 ccattttggt tagtgaatat tcctgtgatc ggtttagttc taatactttc cgctctatgg
     6601 atcgtttatt atcaacgaac gttttggaaa aaatttgaat aaagaagagg ggccgtgaca
     6661 aaaatcatgt cgcagcccct tttttacaga tagagggtgt tcaaaagtca gtttctcagt
     6721 attaagtcaa ttttatgtaa aaatgtgaga agctattttt acaaaacttt ttttaatact
     6781 cataattact tcttattgct cctgcggtta cttgtcgcaa tgtttgtgag atattttgtc
     6841 atcgctcaag gctgaacgtc tctttcacaa ccttttcttt gttacacttc gtttatttaa
     6901 aaagccgttt ttttcgtttc atcatgattg cgtcacgctt tactttgaaa ttacggatgt
     6961 tatggttaaa gacttctgat aatacaagac ctaaagcgat tgctccagca atcataattg
     7021 cttggaccgc ataatgagcc gcttctcgat aggattcagt aacgagattt cgaaccgctt
     7081 gataagctaa accaccaggg actagaggaa ccatcccggg aatattgaaa atcgttacgg
     7141 gcattttcaa acgtttagag aaaagatcgc tgatgaatgc tactcctaaa gaaccaagca
     7201 atgaaccaaa agcagcagat ccgcctattt gaacacagat ccagtagatc atccagccaa
     7261 aagcaccaga aagaccacaa caaatcaatg aacgcctagg tacatttgta ataacggcaa
     7321 atgccgcagt cgctaaaaaa ctaaaagaaa actgaataaa tatcgtcccc atacttgtgt
     7381 tcctttccta ataaaaaagc tggaaaataa aagcaatcgc aaaaccgatc atcgtagcag
     7441 tcatcatcgc ttctgttccg cgagaaactc ctgagacata atgtccagct agcaaatcac
     7501 ggatagcgtt cgtaatttga actccaggaa ctaaaggcat cacacatccg ataatgatta
     7561 gatcctgatt gcttcctaag ccaaaacgta cgctgtagat agcggctatg ccaatcaata
     7621 aagaagataa aaattccgat aaaaatttga ttctaaaaaa tttcaaacta tatagataag
     7681 ctgcgtatcc caatcctcca atgactaaag tcacaggtaa atcggtcatc tttccgccaa
     7741 ataacaccat aattgttcca ctgatcactg ccgcgctaac gatttgcaac cagacaggaa
     7801 agaaagaacg gtctcggtcg attgcttgca gttcgcagta aagttcttct aatgttagct
     7861 ctgaagcaac atatttacgt gaaagatcat ttacttttac tactttttct aaattaatcg
     7921 aacggttggt aatttgttcc attcggatgg tcgaggtacg atcaagacca ataaagagac
     7981 cggtttgagt gacatagctg accaaacgga aatttccaga agctaaagca attcgactca
     8041 tcgtatcttc tacccgatac atctccgcat tgctttccat catgattttc ccagcgagta
     8101 aacaagtgtc cagcaacaga tctaattctt ttcccgtcat agcatcttct ccaataattg
     8161 attgattagt tttattttac tacaaaacat accagaagcg taaagaataa aaaaacttga
     8221 ggaaggaata aaagaggcgt atactgtttt tgtattgcgg gtgtagttta gtggtaaaat
     8281 cccagcttcc caagctgatg tcgcgggttc gattcccgtc acccgcttaa aataggttgt
     8341 gaaaaagctg ctctgcaaca agtaataaga acagccaagc aaaaacaact tcttatgttt
     8401 ttaacttgga tggacttatt accgcagatg caagcttttg cgcaccgttt accaagcggt
     8461 tgtgaaatca gtgtattgtc ttgagtatga agaaaatttt tgaataataa taaatacctt
     8521 acagatagag tggtgactga ttagagacga ggaatccgtc tcttttttgt atttttaagc
     8581 catcttaaaa caggttttat tttttgctgt ttctatttct ttctagtatt tatttatagg
     8641 ggaacatatg ttaagattta gggagctgtt agtaaaggag taaagaaatg agcaaaaaaa
     8701 caaccaaggc gatcgtgggg atcgtcgctg ctgtgattgc aggggcattc ggtgtttcca
     8761 ccacacaaaa agattcagat atctttcagc agattacaac tatttttgat tccacaccac
     8821 aagcttctca agtagcggat gtgccagaat atgatggaaa acatcaagaa atagaaatca
     8881 atgggaatca gccggaattt acaaaagagg atcttagcct ttccaaagga acatgggaaa
     8941 gctattcaaa tattgataaa ttgaacagag taggacaagc caatgctatg cttggaaaag
     9001 atttgttccc tactgaaaag agagagcctt tgtatattga cccgacaggt tggaaacaaa
     9061 agaaattaag tgacgggcaa tggttatata atcgcagcca tttaattggt ttccagctga
     9121 ctggtcaaaa taacaacatt aaaaacttga tgacagggac acgttcgttg aatgctccat
     9181 acatgcttgc tcacgaaaat gatattgcgg catatatcaa agaaacaaat catcatgtac
     9241 gctaccgtgt cactcctcat tttgagggga atgaactggt agccagggga gtgcaattgg
     9301 aagcagaaag cattgaagac aaaaaaattg aattcaatgt ctttatctac aatgttcaag
     9361 atggctacac gatcaattat gagacaggac aagcaacaaa aacaaaaaat tagtagcagc
     9421 tattcgaatt ttttatgaat attatgaaaa aattgtgtaa aaacatacat tcaaagaaat
     9481 atagcgtata ctttatgaaa agagtacttt tgataaagct acagaaaaaa ggaaaaaaac
     9541 atacaaatag tgtttcttaa tttattcgga agtttggaat atttgtttgt agttttccct
     9601 ttcttcgagt aaaataaggt tgtagaaggt aagctggaat taccttgcgg gaaagaggtg
     9661 tagaatcatg ggttttacag atgaaaccgt acgttttggt tttgacgata gtcgcaaaaa
     9721 agaagtgagc gaaacattag cgattgttta tcgggctttg gaagaaaaag gctacaatcc
     9781 aatcaatcag attgtcggct acttgctttc tggagacccg tcctacattc ctcgttatcg
     9841 tgatgctcga aatttgattc gccgtcacga acgcgatgag atcatggaag taattgtcaa
     9901 ggactacctc tcaaatcatg gagtgaattt atgagagtaa tgggcttgga tgtcggttct
     9961 aagacagtag gtgttgctat cagtgacccc tttggctgga cagctcaagg agtagagatc
    10021 attcggatca atgaagcaga agaagagttt ggctttgatc ggttgaagga attagtagca
    10081 caatatgaag tagaccggtt tgtagttgga ttacctaaaa atatgaacaa ttccatcgga
    10141 ccaagggcag aagcttccat gtcatacgga gaaaagatca aagaactatt ctccttgcca
    10201 gttgattatc aagatgaacg attaaccact gttcaggcag aacgtatgct agtagagcag
    10261 gcaaatactt caagagccaa acgaaaaaaa gtcatcgata aattagcagc tgtgatgatc
    10321 ttacaaaatt atttagacgg ctcaggtaag caattatttt aataataaaa gaaaagaggt
    10381 aatcttatga cagaacataa ccattcccac gaccatgaag gacacgaaca catcacattg
    10441 gtggatgacc aaggaaatga aacattatat gaaatcctac taacagttga cggacaagaa
    10501 gaatttggac gtaactatgt tcttttatat cctgctggtg tttcagaaga tgaagatgta
    10561 gaattacaag cttatgcata catcgaaaac gaagatggaa cagaaggcga gttagaacaa
    10621 atcgaaacag acgcagaatg ggacatgatt gaagaagtat tcaatacatt catggcggaa
    10681 gaagaagaat agactataaa gctaggagaa accttgatat gatagggttt ctcctttttt
    10741 tattttttac tgtatgccat attgtatgcc atctagtcaa tatttaagtg ttgtactaat
    10801 ttttcaacag tcttttcttt cgtttctttt gccaagtgac cgtaacaatc gatcgtctga
    10861 ctaatttttg tatgccgtaa tctagtttga acttctacca tagtagctcc agcgtccaag
    10921 agcattgaag catgagtgtg tcttgctaca tgcacaccga gaaatggaac catagctttt
    10981 tcacaaatag taatcaatct tgcataaata gcgtggtgtg tgattagttc tttatagata
    11041 ccacaaaaaa tcatagtcgg atcagttaca ccaagtgtca gcatatattt ccgctgatta
    11101 atctgccatt tttttaatat agaaattgtt ctttcatcta gtggaagaac cccagcacta
    11161 caatcagttt taggatcact gattttatag ccatttttgg attggcttag tgttttattg
    11221 atggtgaccg tattttcttg aaaatcaatg tctgaccaat tcaatgcaag ggcttcactg
    11281 atcctaaccc cgctataaaa gagaaagcgt agtaacgttt tatcaaagtc gtttcgataa
    11341 gtttcaggtt tgctgtttag gtaatcaaac aaggtgatca gttgttcttt actgtaaact
    11401 ttgacctttt ctttatgctt agtggtggtt cttttaggca ttaatacgtt gtctagtgga
    11461 ttagtctcaa taatcccaag attgatagcg tagctaaaaa tacgcttaga agttgaatga
    11521 agtaccttgt attggtcagt agtttcagcc cagctattta caatttgttg ggcttttttt
    11581 accgtgactt cattcacacg aagatttcca tattttggaa gaatccactt tctaaacttg
    11641 atttcagttt tcatgaaggt ggcttctcgg acagtggttt tatatgtttc tagccacaag
    11701 tcataaactt ctttaaacgt ttcgttgttc gcttttttaa taccgttttt atcgtagtcc
    11761 acttgcaagc gtgacaaggc aagctgggct tctttttttg ttttgtaacc tcgtctacga
    11821 accttatcag gtttgccagt cattttgttc attccaagat aggcttcata gagccaaaat
    11881 ttaccttttt tagtttcata ttgcttgaat gttgccataa tagttttccc tccatagtgt
    11941 agcaagccgt gggggcggtt ggaggaggca gtttattatt attttgaata gtgtttattt
    12001 agctcagtaa caagagctgt gaactctttt agaagatcat tttcataaaa ttcttgatac
    12061 tcttttttct catctgtatt tttttctgaa tcattaatta atgagtcata tcgtaaaagc
    12121 gtatttagaa gagctgcgag ataagcaatt ggggtactcc cgtcaggaaa ttcaattcga
    12181 gatcctgttt taagatattt tattgcgttt gttaaataaa tcttctgtga gacagtcagg
    12241 ttatcttctt cgatgttatt gatagcatta gaaagattag aatcaattat agagttattt
    12301 ttttcaatag tttttttcaa ttgcttttca agcacatttc gcttaatttc gggcatcata
    12361 tgcatatctc ttaatgtata agatccgttt aatagatagg tagtggatac atctaatacc
    12421 tttgaaagtt gtttaagcct ggtgttattg ggaagattat agccattttc ccaattacta
    12481 acggcacctt tactggccgg tggggataat ttacctccaa gttgttccat tgagagtccc
    12541 ttttcaattc tagccagtct aattctttgt cctgtctcca ctgcttcggg gtattttttt
    12601 ctagccacat tttcaccacc attcatatat tttctctact atatcacgat ttaaatatgt
    12661 gtgaaacata aaagttcaaa aaagtatgtt aaaattcttg caaatgaaaa ggtatgatgg
    12721 tatattgagt ttataagagg tatagaaatg tattcaagag cctcttgcat ttttttaaaa
    12781 tctttgtata gaaatgtatg ctaacgaggg gggataaata tggaattaaa aattgacaaa
    12841 ctcacgcttg atgatccagc ttttaaaatt attgacgatc gagttgttca acaagtagag
    12901 caaactcaag gaacgttact ttcattatta gaaaaaaagt accagtttcc aaaatatatg
    12961 aacaaggcac aagctgctaa gtatatgaac gtttcataca atactatgat gcagaaatat
    13021 gttcctaacg gtttaaagtt gattatagtt gatggagtcg taagaatttc acaagatgaa
    13081 tgtgatcgct ttatggaaga ataccaaaaa taaacgcaag ccgtgggggc aaagggatta
    13141 ataggaggaa ataataatgg aatcgacaca aaataaagca atcgaaaaag tattatcaac
    13201 aattcttaca gaagacacag cggcagaatt agcaaacttg gaaggcaaag cgctagaaga
    13261 aacgtttgaa tgtttgtacg aacaaatgga ctatcaaaaa ttgctaccac aagaaccaac
    13321 ggtaagcgga gtattgcgtg gcttgaacga tttagtacaa gcagagttta aagaacgact
    13381 ttctattgaa gagtatcaag aaattttgta tcagcaagtt gagttgttag cgagcttgct
    13441 agggcttgaa ctggaggacg ttgaatgaaa actgaaattt ggaacggaca cattataaga
    13501 tttgtcgata ttaatgatga atggtgggca gtggcgaaag atgttgcgga agcgttaggt
    13561 cttaaacagg ttactagagc cattcactct ttgcctaaag atggggttac tactagtaag
    13621 gtcatcgatt cattgggcag aacacaagat gtaaacatca tcaacgaaaa aaaatattta
    13681 ccgcatggca ttcaaaagcc gtaaaaaaga agccgaggca tttcaagact ggattttcga
    13741 catcatcaaa gaactacgcc agtccacagg acttgaaggc tttcaagtat tccgcatgct
    13801 agacaaagaa caccaaaaag aagcaatgac taaattaagc cacgctatta ctgaaccaaa
    13861 accagtagac tacattaaag cgaatgtgat agccaataaa gctgtatcaa ctatttatgg
    13921 tcattccaaa atggtaaaaa agaaagatat gactcctgaa atgttggttg atcgtgaacc
    13981 tattttagat gagacagtag aattaatgac tgttaaagaa aaatatgggt tgcagttttc
    14041 agtgagtgag aaaatttata atcgttctgc tgaattgcag acaacttagg agcattaaca
    14101 tgatcgagaa caaagaaaaa gccttatcca taacagtctt ggcggacagg ataaaggcaa
    14161 cttaattggg ataaaacccg atattctttg ttccgattat accatattaa aaaatcggag
    14221 ggaaccatta tgaaacgaaa cttaattata ggaagcattt taatcggcgg aattttcggc
    14281 ttattaccat taattgcaaa aatagctatc tttgcaggat tagcgttata tctcttcact
    14341 gcttatgacg agtacgacta tcaattaaga actggagggc ataagtagat gtacaaaaca
    14401 aaactattaa accaactcga tagccttgaa ttagaggaaa ttaatcaagg gatcgctgaa
    14461 ctagaaaata atatcgggaa aacctacttt ggaaattcat tcaatgaaaa actaacagtt
    14521 ttgtatgtac tcaaaaaaca tgcagaacac aaaataattt gtcgagaaat caacgagcta
    14581 aaaaatcaga ttttaaccgc atggttaaac attactgata tgcaagaagc aagggtgaaa
    14641 acatttaata catgggtaaa gtatcagaat caattaaaag gtgctgaatt tgttcgggat
    14701 gggttgaaat acgaacttga gcagttgaag ctcatggagg tgtctgaatg atttatacca
    14761 gtattttgaa aaaggaggag aggtttattg gcaaataatt atcaagcagc aacggaatta
    14821 caaaagtcgg gatggtcaat ttatccctta tctcctggaa ctaaaattcc aataaaaggc
    14881 tctagtggtt ggaatgatgc aactaacgat tctaatcaaa ttaatgaatg gtggacggaa
    14941 gatgcaacta gaaatattgg gttaatgctt gaagatcatc aaatggtggt agttgaccta
    15001 gatcaacatt ctgaaacacg aaacggcgtt gagaattata aaaaacttgc ttcaatgtat
    15061 gatccatttc cttctactta tacggaaatc acaccaggta aaggaatcca tttcttcttt
    15121 aaaattcctg atggtgtaat tatccaacaa gaaacaggtg catttgctga tatcttcggg
    15181 cgtgatgata aaggtaaaag tttaagcggg attgatatta tcactaaagg agttccaata
    15241 gcgccaacta tgattatttc aagaagtgtt gggagagcat atgaacctat tgaaaacgta
    15301 tctctaaata atattgcacc agctcccgat tggctagtga acctactgct aaaaaaaccg
    15361 caagaaataa agactcaata ccgacccgta gcaaaaacag ggacagcaaa aaaattagat
    15421 atgattgttc aaggcgctgg cgaaggtagt agaaacgatc atttaactaa gttatgcggt
    15481 tggttactat ggcacggtgt agataatcaa actttagcag aattgattta tacagcaaat
    15541 gcgtacaatg aaccaccgct aaaagataaa gaagtgaatc aaattattcg atcgatgatt
    15601 aaaaaagatt tgagggggaa agctcatggc tcataagcat aaattagata gtattttaga
    15661 ctttcccgaa gccagcgagc gtgaagacaa tatcattgaa ttgaaaacct ggatgtcacg
    15721 attaagatgc aataaagacg atcagattaa aagtaacagt gttgtgaatg cagaattgat
    15781 tttaaccaat gatagcaatt tagctggaac aattgcttat aacgaattta gcggatacat
    15841 tcacctatta aaagattctc cttggatcaa tcgtaccgct ggagaatggg aagacagttt
    15901 tgaggatgcc ttaacggcat atattgaaga aaactacaat gtagtttttg atgacaacaa
    15961 aatacacaaa gcagtagtaa atgtggctag aaagaatgtt ttcaacccag taaaagaacg
    16021 tatcgaaaaa gttaaatggg atcaacagcc tagactggaa acactgttta ttgacctttt
    16081 aggggtagac gacaaccttt acacacgaga agtaacaaaa cgttggatag tcggatcagt
    16141 cgcacgtatc tataaaccag gaataaagtt tgagatcgtt ccagttttgg atggaccgca
    16201 agggattggt aagagtaccg ttccagcatt actttacacg gatgattttt tcaccgattc
    16261 attagattcg ctaggtgaaa agaaagatga ttatatgcag cttcaaggca atgtaattat
    16321 tgaacttgga gaattatctt caatgaacaa aactaaaatt gaacagataa aaaacttcat
    16381 cagtgccaag atcgataaaa ttagacctcc ttatggtcgg aatgtcatag cttgggcaag
    16441 gcaatgtgta tttattggaa cgtcgaatga tgggcagtat ttgaaagatg atacaggaaa
    16501 ccgccgtttt tatcctttgc cttgtaagaa taagccaaag atgaatccgt ttaaaacaaa
    16561 ggatgaatat tttcttcaag tgttagctga agcaaaggtg ctttttgata aaggacaacg
    16621 aatctatttt agtcccgatg aagataaaga agtcttagaa attgcaaaag actatcaaga
    16681 ggatgccaag atggagaatc caattaaaga agcaatcact aaatatttgg aaatggaaat
    16741 cccgtataaa tgggaagaag ctccagcgtg ggcgagacgt tcttattatc agaactatcc
    16801 tgacagtaaa tgtaatgaga aaaatttaca gcatttctat aatggagcta ctatgcaaca
    16861 agagttttat ttagttgata gcgtactcac cgctgatatt ttggaagctg tttttgataa
    16921 acaagcaaag gatttattga atggtcgatc agatgcagaa actaaaaaga ttgccttgat
    16981 tattagcagt attcctggtt gggaacggaa gcaactgcca aaaagaaata aacgacgagg
    17041 attttacaat aagtcaaatg cctatgaaaa taaaaatcga gctggaaagt acaaaaagta
    17101 acgggtagta acgggtcagt aacacctagc ccgttactag tttaccctta ctgccccaag
    17161 ggatacagca atttggtaac ggggtaacac cttatttcct taaaactttt gaaatagtta
    17221 gctaatatgc tggggaataa tgttactgga ttttacccgt tacctgttac caaaacgcta
    17281 gaacccttgt ccccgttggg attgagtggt aacgggtacc tgttacgtag gtgttaccac
    17341 gtgttacgaa tcaaggagga aagtaaatgc tcaatattat ttcaacaaac aaagcaccga
    17401 actttcaata tacagatgaa atggatcggt ttctaatgaa cacgctggca tttagtgtgg
    17461 gattggtaac agaagattat tccacatttg atccagaagt attaaagata atggaagaag
    17521 aacctgattg gctgcaggag tcagtagcgt ggtgtcaatc gctagtagtg ggatcattgg
    17581 tagatagtgg caactatgat gatacaggtg agctaatgga tgaatttaat tgtctgctta
    17641 acctatatga tcgagcgaga caacgagagc ttacatcgaa tgaggataat ttgtttttaa
    17701 acatccatga taagttcttg gctttgctac taacagatga tgaattgata actaatttat
    17761 tggaggtaga ataattatgc atatggaaag tattagatac ttcgatggtg agaaacacat
    17821 tgagtattca aacactcaag aagatgtaga ctttattagt tttgaggaag ataaaatcac
    17881 taaagttaat tttaaagacg gaacgtgctt gaagatagtt tcgccttata ttgaatacaa
    17941 atcaaaatgg gaacaagatg attctgacga acgagatcgc tttggttggt aatctatggc
    18001 agttaaaaaa caatgtaatc atgctggatg taaaacattg attgattata ggcagaagta
    18061 ttgtgggaag cataaagcaa agcaaacggc aattaagcgt gaagaaagaa agcaaagcga
    18121 aggtaaatat tttcaattct atcaaagtag aacatggcga aaggcttcat acttgtatcg
    18181 gttaaatcat ccagtatgtg aggattgcct agaagaaggc ttgataagga aagctgatgt
    18241 tgtagatcac aaaattgaac taaaagatga ttggaataga cggttagatg aaactaattt
    18301 tcgttcttta tgccatgcac accataattc caagacagcc aatgaaagac aaagacgaga
    18361 aaagagtacc ctttgagtta agggggcata gtgtgaaact tactgacaat cgatgcctac
    18421 tcattttggt acaaataacc gttgtgaaaa ggcataaggg taattacaaa taacgattac
    18481 acttgtaatc acaagtgaaa aagtatataa tgaaagtagg agatttattg aaaaatacaa
    18541 ttgttattac tgataaaaca gggaaacagc gcaccattga gttacctcat tttggtcaag
    18601 tgaccattca aatgcagaac ggaaaaatca tttatataga taaattagat aaagaaaaat
    18661 tctgatcgaa aaacgaagga atgtgagctt aattgcttac gttccttttt cttttgtctg
    18721 aaaggaggac aacatgggac accctaaaat attacacgat acaaaaggca atatatcaag
    18781 tgaagaaaaa gcaatccgtg tggacgctag agaagaattg ttcaagcagc aaccattaat
    18841 aaatatcacg ccccctgact ggatggcagc gagcgctaga agtgaatgga atcgtatcgt
    18901 accaacatta aaaaaagatt atccattgag tgaagcagat tatggttcat tggtagccta
    18961 ttgtttagcc tttgctcgaa tgaaaacagc cgaagccgag ataagaaaat caggtacgtt
    19021 tatcacatgc gaaaacggag taaagaaagc caatccagcg gttcgggttc aatcacaagc
    19081 tatgagtgat ttgaaaaaac aagccacctc actaggtatg accttagaat cacgatcaaa
    19141 actagctttg aacaaggtta aaaatgatga acccgaagac ccgttcaaag agttgatgaa
    19201 ttcatgaatg attacattga gaaagtccta tcaggtaagc tgattgcacc caagaaaatt
    19261 atccaagcgt gtgagcgtca tataagcgat ttggagcgtt ctaagtcaga tagcttccct
    19321 tatgtgttcg atgaagaaca agccaccaaa gcgattaaat tcattgagtt gctaccatct
    19381 acggacggta aagcaatcaa gatgttagga tttcaaaaat tcattctagg cagtctttat
    19441 ggctggcgta ctaaagaagg gaattacagg cgattcaatc gagcgtttac cagtatgagc
    19501 cgtaagaatg ggaaaacgta tatcgcaagt ggcatggctg ccaatgcgtt gattatggaa
    19561 caagaaccag cagaagcaag gcaagtattg tttgtaagta acgctttgaa acaagctaaa
    19621 ttgggctatg atatgctgtc taattcactt agaaacgtgg tcaagtctag taagttttta
    19681 agaccacaac tgaaaattat gaactctaag attcagcact tgccctctaa ttcgttcgct
    19741 atggcactgg ctagtgaaac cagcacgcta gatgggtttg caccaacaac cgcaattctt
    19801 gacgagtggc acgaagcaaa aactcgtaaa acgtacaacg tcattaagtc aggaatgacc
    19861 caacaaaaga acggcttatt gtgtgttatt agtaccgctg ggcttgattt aaacgttcct
    19921 atgtacgagg aatacttatt gttagatcgt gtgctaaaag gcgaagaaca agccgacagg
    19981 tactttatag cgatatggga attggacgat cccgaagaaa ttcacgatca agagaaatgg
    20041 atcaaagcca atccgatttt tgaaagtgaa gaaatcaaaa aagtaatgat tccaaccatt
    20101 caagatgatg tgaaccttgc tttgaaacaa aataacctta attctgtatt ggtgaaaaac
    20161 ttcaatttat ggagacaagc gagtgaggac agctatatga ttgctgaaga ctggcaggca
    20221 accgaagtag aaccacaaga tattacaggc aagcccgttt atatcggagt ggatttatct
    20281 aaaacagatg atttaactag cgtttcatgg atcgtaccgc tagataacgg cgaactttat
    20341 tgtgattcac atagctttgt agccaccaaa tatgggcttc aagacaaaga aaagcgcgac
    20401 ggtttgcctt atcgagaact agaaaaagct ggtgagtgtt ccattactca attagaaagt
    20461 ggaatcgtgg actatgacca agtatttcag tttattcaag atttgattca agaaaatgat
    20521 ttggaatgta tggggatttg ttatgacccg tataacgcta attcgcttat cagtaaagct
    20581 gaaaaagcca actacccaat gttagaagta agacaaggaa cgattactct aaacgttccg
    20641 acccgaactt ttagagaaca agtttatgaa ggcaacgtta ttcacaataa aaatacgatt
    20701 ctcacccatg cagtgaacaa cgctatttta aaaacggata acaacggcat tcagattaat
    20761 aaatcaaaga acagtaacaa aattgatcca atagctgcat taatcaatgc ctatgtgttt
    20821 gcaatggatt acttcaccac aacggaagga gcgaaagcag acaatgaatt ttatacaagt
    20881 gaagaatttt ctttctaatt acattcatac cgttctttta cttcttggat tggtgtgtgt
    20941 gttggttgct atcaccttac taacaaatgt ctactatggc ttgttagcgc tgggcatagt
    21001 gcttattggg gtagcggtca tgctaaatac agaacagaag ggaggttaaa agatggcatt
    21061 ttttaaagcg agacaaaata caacgggaga tcctttccta caacatgtgg tatcaatcca
    21121 atcggatgat tacaccacca gctttacaag cgttcgtgca ttaagaaata gtgatgtgtt
    21181 tgcagccgtt cgaattattg ctagtgatat tgcttcaagt ccgattcaat tggtcaaaaa
    21241 caatatgcca caagctgata atgaactggt gaagttacta aacgagaaac ctaattcaga
    21301 aatggatggt tggcatttaa agtttgcttt ggcggtcaat atgttgctaa acggtaatag
    21361 ctttgcagag attaaacgca acggtgaaaa ggtagaagaa attcacttat tacctaactc
    21421 aagtgtaacg gttactcaat tagataatgg tacgttgtct tatcagattg gtgataaaaa
    21481 aagacgtgtg aagtctagcg atattttgca cttcaaatat ttcacccaag atggtttaac
    21541 aggattacca ccactttatg cgttacgtga tgaactgaaa attcaacaag ctggcaaccg
    21601 cacattgcac aatttcttta ctcgtggtgt cagtgggtca ggtattttga aggttcataa
    21661 gtcagattta gacggatcag caaagaacgc aattcgtgaa aagtttgaag aagccaacgg
    21721 ttcaagtagt ggagataatg ctcttagaac gatcattctt gatgaaacaa tggactacaa
    21781 aacattagaa ataaacaccg atgttttgaa gcttatcaat tccaatgatt ggaacacaaa
    21841 acaaattgcc aaggcgtttg gtgtaccgat tgagcgttta ggcgttgaaa atgaacattc
    21901 tagcaccgtt caaagtaatc tccagtatat ccaaagtacg ctgattcatt actttaatgt
    21961 gtttgtgagt gaattagata cgaaacttga aaccaatatc cgctttaatt ccgatcagtt
    22021 actagagact gatcctgaaa caaaagtaaa aaatgtattg gatcaggtca aagggtcact
    22081 tctcacgatt aatgaggggc ggtcgaaaat ggggctaccc cccatggatg gtggcgatcg
    22141 tttactagca agtttgaact ttacgtattt agatacgttg gagaaatatc aattaaaaga
    22201 acaggaagga gttacaccag ttgaataatg aagaagaaaa ggaaaaacgg ctgacagaag
    22261 aagctgagct aacagccgat tctcccaaag tagagaaaga aaatgaaaaa caaccaacag
    22321 acggcaaaac tatttcaggc tatgcgttga aattcgggca accgtcaaaa gatttaggcg
    22381 gctttgtaga agtgattaca cccgaagcat taaaagaggt ggatttatca aatgtgttct
    22441 tattatatgg acacgattat tcaaagccac tagctagtgt caaagcaggt acgttgaaat
    22501 tgaatgtcga tgatacagga ttacattttg aagcagagct aaccgacacg acctattcaa
    22561 atgatgtgta tgagaatatc tcaaaagggg ttgttgattc tatgtccttt gggttcgtgc
    22621 ttggattaga ttcattcgac aaaaaagaag atggcacaat tgaacgatca atagataaaa
    22681 tcaaagcact taatgaaatt agtgtcgtga ccgttcccgc ttatgattca tcaaatgtcc
    22741 aagtaaataa gcgttcttac gaatcgttta tgagtaacaa ccaagcaaaa caaacaaaca
    22801 atagcttaga atccacttct aaagcacaaa aggagagtaa taacatggaa aaaactttaa
    22861 tcgataacga aaaaacagaa ttgcgtgggt atgaagaata tatccgttca caaggcgaag
    22921 tgcgtgatgg agtcactact gtaaatgcag cggcagttgt tcccgaagaa gtaatcggtg
    22981 aagtctttga tttgaaacgt tcaaattata acttagctca atatgcaaca gtaaaaacag
    23041 tatcaaatgg acaaggtaaa tatccagtag caactaacca acaagcagtg ttagcaacaa
    23101 aagctgaact tgctgaaatt ggtgatattg acgctgaaat gtttacttca gttgattata
    23161 aagtagaaac tcgtgctggt aagattgcct tatcaaatga ggttgtggaa gattcagcag
    23221 tgaatattgt acaagaggtc aaagatcagt tagcgaaatt ggtagaaaac actgacaata
    23281 agcatatcat ggatttatta aaaacattca ctaagaaaac ggctgctacg ttggacgatt
    23341 tgaaacaact atacaatgtg gcattagacc cagcattaaa taaaatggta attctaaacc
    23401 aaagcggata ttaccaccta gatacattga aagattcaga tggacgttac attttacaac
    23461 ccgatgtgac agcacctagt ggtaaatcat tattcggtat gccagtagta ttgattgcag
    23521 atacattgtt tgccaatcct aaagctggaa cattccctat gattatgggg gatattgcac
    23581 aatctatctt tgttgctcgt agaaatcaag taacgactca atgggaaaaa ttcgattact
    23641 actcacaagg acttgcagtg atcgttcgca acgattataa gaaaattgat caaaatgctt
    23701 cagtgtatat tgagtttacg ccagttgtaa caccaaaagt atagaaaaat attgggcggc
    23761 ggtttatccc actgcctttt tttcattagg agagataaaa tggtaatttt ggatagcata
    23821 aaaaaaagta tgcggatcga tcacactatt gatgatgact ttattcaaca attgattgat
    23881 acagcaggcg aatatataaa aagtgctatt gatagtagcg caacggataa agatatggat
    23941 aattatcagc aatttgattt ggcggtgtca ttacttactc aacattggta tttgaaccgt
    24001 caagaagcca gcagcgaacg gataccagta acggtacaag cattagtaca acaaatgaga
    24061 ggtgcttatt atgccaatca ttaagaatgt aaatgaattg actgagagag tcaagtttaa
    24121 aaagacaaaa cgggtaaaag atgaagatgg gcagatggtg gatagtgaag aaactgtatt
    24181 tgaatgttgg gctaatgtgc gttcacaaat gctaaaagac gtgcttgcta gtgtaggtac
    24241 cattcttgaa ggaacgttga cgtttattat tcgatacgat caagattatg aactaacaaa
    24301 cgatatgaaa gtagcttgga aaaacaagag ttataaaatt atttcaatca atgaaggaac
    24361 ggcgtttaaa gattatacaa cgatcatagc taaacagatt tcttagaatg attacacttg
    24421 taaacgttgt gggtatttag tataattaag gtagtaaatg aagggttagc tgcctgaatt
    24481 ttgcaagact tagctagtcg aaatctattg ttggactgaa aattgtaggt gtggttgcaa
    24541 acatgctgga ctaagaaaga tgatagatga tgaacgttca attttaatac aggcatgcta
    24601 ggactgattg gatggttttg gcaagctgat tgtgtagaaa tcaaagaaca cgtctgttgt
    24661 ggacgtgttt tttttgttat aattcaaaag aggtgattta atggacaaag cagagtttac
    24721 aaatttttat tggaagtatt atctacattt agaaaatgag tttattaata ccacagattt
    24781 tgtgatgctc gatgagaata atttcaaaac attctctatt gaatatcaaa aactactact
    24841 agcgattggt tcggagtgtg agattatttt caaagaactt tgcggatttg atagcagtag
    24901 caataaaagc ataactgatt ttaaagctgt tattaaatct agcgaattaa tgtgtttgga
    24961 taatggtgtt agggttatga attctttaac attgacttca ctaaaacctt ttggagatgg
    25021 gtggcctgaa gatacaccag attggtggaa aatatataac agagtaaaac atggtcgttc
    25081 ttcaaattat aaggaagcaa atttggaaaa tgtgttgtat gctttagcaa gtctatattt
    25141 attagaggaa tatttgcata agaaagtaat actagaaggt gaagtagact ttataagtcc
    25201 agagtcaaat ctatttacgt tatcttggga aagaaaacat tcgtcgatgc aaaaagtgac
    25261 gtttgaaaaa gtggattcaa attatgtagc tccactcatt gattttaaag aacaagaaga
    25321 aatttaaaaa cccccacggc ttattgaagt ttttgtatgc catattgtat gccatttggt
    25381 ttaaatcgct tgaaaaatag tgatagataa ctctaattca tttagtcata aacgctgttt
    25441 tgataacctt tatagttctt tgatatgggt ttctcattca tggcggaaga agaagaatag
    25501 aacaaaagaa tgaattaata agatataaaa aaagggattg tgacaaacgt cttgtcacaa
    25561 tccctttttc caaataaacg atgttcaatc agctgcccct cggcattcgc tcgaaaaacg
    25621 gcgaaatatg aagaactatt ttgccatttg tttctctaat aatcaggtca aaacgttgac
    25681 ttcacagtct cttccttaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    25741 ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga gctgaacggc
    25801 aggctcacga cctctttctt aaacggtgtt caaaagcttg catctgcggt aataagttca
    25861 accaaacgaa aattatgagg agctattttt gtttggctgt tcttattact tgatgcagga
    25921 cagctttttc acaacctctt ttcgtttagc actaaatttc cttaaaaagt agaaaagctt
    25981 tcgaagcctc gtttgtttct tttactgtat aataaaagaa aagagtaaca atcgctaaaa
    26041 gatataaaca agagaaagga aatacaagat gattggtctg atttattttg tggtgatcgt
    26101 tctggcgaac acagttggcg ctatttccgg gatgggtgga ggcgtgttga tcaaaccgat
    26161 tcttgatctt atcggcgccc attctgttgc agggatttct ttttactcaa cagttgcggt
    26221 attcacgatg tccattgttt cgactgtgcg acaagtatca agcgggaaat cactgaattg
    26281 gcagattgtc ggttgggtat ctagtggagc tgttgttgga ggagtagtcg gcaacattgt
    26341 attcgaagtt tttttacagt tatttgaaaa cgagaagcat gtgcagctga tccaaatttt
    26401 tttgacggta ttgaccttgg tttttgcgtt cttctacacc aagcatcatc agccaaaatt
    26461 tcatttgacg tcatggactt ggtaccttat atgtgggggg gttctgggct ttttagctag
    26521 ttttttaggt attggtggtg gaccagtcaa tgtttctttg ttgatgctga tgtttgcatt
    26581 gccaataaaa gaagcaacac tatattctct atctacgatt ttcttttctc aactggcaaa
    26641 attggtaacg attgctttaa caagtagctt tatgcgcttt gatttatcta tgttgtttta
    26701 tgttatcccc gctgctgtta ttggaggttt gtggggagca agatttagca gggttttgtc
    26761 tccaaaaaaa gtgaccttta tttttcaagt aattgtcctc gtggttcttt tgatcaatct
    26821 ttacaatgct ttcgtcatct tacaaacatt aggagaatct agactaacgc aagtcaaaat
    26881 cactgaaaca tattgttcta aatgaaaaaa ggagtatact gtgataaaaa aattcattta
    26941 aaaaaataaa aattggctgg tgatttggat gttgcgggaa atgcgtttag gagatgcaga
    27001 agcactacga gaaatcaatg cggaacaatt aggatatgat gtaccattaa ccgttacggt
    27061 gagacaaatg aagaaactgt tgaaggagcc agaaaaaaat tttttcttgg tctatgaaga
    27121 tcctacttct aaaaaagcag ctggctatgt acaagcggag tcctatgaat cgattttcag
    27181 cgatccgatg ttcaatatta tggcacttgc tgtgtctaaa catgctgaaa aaagaggaat
    27241 cggaaaggcc ttgatgactg gcgtagaaga agcgaaaaga cgaggaatat cagcaataag
    27301 acttaattct gcagaataca gagtagaagc acaccgattt tatgaacgca tcggttatca
    27361 ttccgataaa atgcaaaaaa gatttttgaa aatcctgtaa atttttatca aaaaatacct
    27421 ggtcatcaag ccgatacttg atgatcaggt attttttttg actaagaaag taattgcatc
    27481 aagaaacctg agatgatcaa aacgatggca ccaccaagcc gattgcccat ttgagcaaaa
    27541 gcaataagtt ccatccgatt agctgcagat aagacagcta catttcctgt accacccatc
    27601 gagttgttgc ataagccagc agtaatagtt gattcgattg gatataaacc aaacaaacga
    27661 ccgacaaatc ctgaaactac tgaaatcgcg ataacacttg tcaaacataa gacaacaaat
    27721 tgccatgtca aagcagaagc caatacattt agattcaata acgcaatacc gataccggca
    27781 agaacagctg ctgtcaagtt tttgacaatc aagttgttga acatgatagc tgcatcttcg
    27841 taatattctg gtaataggtt tgcgatttta cagataacaa cgataatgat catgaatgcg
    27901 taagcatgga ctttaggaac aaaatagtta cagatttgtc ctaagatgaa gaaagtcaat
    27961 gataccatca aaccgacacc taattgtgga atggaaggtt ttacatttct aggttttcca
    28021 tcgccaaggt cgcctttttc cattaattta ccattaccgt tgaattttgg cattgattgt
    28081 cccgcacgag caactaacgc tgcaccaatg atagctagga catttgtcat tgctgtcgca
    28141 ggaatcaggc gggaaatgat agaagaagcg tccgtaccta aaacatttgc ataaatcgtt
    28201 gataaaggta cagctccggc tccaacacca ccagccattg cgggcaatga gatatataaa
    28261 acggaatttg caaaaccatt tccgataagc atacctacga gtcctacggc aaaaaaggag
    28321 acgaccattg aaataagtgc aacgggaata aaacgaacag aagctttcat caatagatta
    28381 cggttcatac caaggatgct accagtaatc aaggcagcaa tataaaagtc taaaaagccc
    28441 atattgctga caaagttttc tgtggatttt actacatact caggtaagat cccaaatgta
    28501 gcaatcgcgg cagaggcaaa gatcgcaaaa acggagccac cacctaaata acttttgacg
    28561 atagggattt ttgttccgat aaaatgaaat aaattaccga aaatgaccaa aacagcgatt
    28621 gggccaatca tattcgtcgg taattttcct aaagccattg caatgatcag gacaaggacc
    28681 atgatcaaat aaaggggcaa acttacgccg gtaatttttg ttgaccaaaa cccttctttt
    28741 ttcttttcct caactgcagt tgcggttgtt gttttttgtg ccatttttac agatcctttc
    28801 ttattctgta tcgtatgaaa aaagccaaag agaagcggca aaagagcagt agttggctgg
    28861 ctgctgtttt gccgcagtcg cttttttctt tttagacgtc atacaaagtt tctgtatgtt
    28921 cttttactat aacaatctta atcttctaaa gatttatatt caggaaccca tttagcagat
    28981 tcaacagctt ctttgatatc cgtgatttct tcacgattca atttttgtgc aacaactgat
    29041 tttgcgacag tttcagcaat cgtttgagag aattcagtga tttttgctac tggaggcaag
    29101 atagctgctc ctggttttgt tacatcaacg attccgccta atgctcgact agcttgagaa
    29161 atgatttcag catttactcg tgtagctgtt gaagcaatca aaccaagccc taaacctggg
    29221 tacatcaacg cattatttgc ttgaccgatt tggtaagtta caccgttata ttccacatca
    29281 gcagcaggga tacctgttcc aactaaagct tttccgtccg tccattcaat caaatctttt
    29341 gcttttgctt ctgccaattt tgttggatta gacaaaggca tgatgattgg acgtggggta
    29401 tgagccgcca tttctttcac gatttcttca gtgaatgctc caggttgggt agaagtaccg
    29461 atcatgatcg ttggatggat tgcttttaca actgcttcta agtttgtcaa ttcatcagca
    29521 ttagtgaatt ctgagcgttt acgagcgaat gggatttgac caggagtcaa tccttttgta
    29581 tcttcaaaca ataaaccctg tttatctact tgatagaaat gttggcgtgc ttcttcttca
    29641 ggtacacctt gacggatcat ttcatctaaa aggatgtttg cgatacctac accagcagta
    29701 ccagcaccaa atgtcaagat cgtttgatct ttcaattgct cacctgatat attcaatcca
    29761 cctaatacac cagctagtac aacgattcct gtgccttgga tatcatcatt gaaagtagtg
    29821 attttatctt cgtatttttc taagatcgtt gcagcatttc ctcgtccaaa atcttcccaa
    29881 tgtaataaca attctgggaa taattcagta gctgattgaa cgaattgatc aatgaattcg
    29941 tagtaagttt cgccttctac acgagcatga cgattcccta agtataatgg attattcaat
    30001 aattcttcgt tattagtacc agcgtcgatt gatacaggca agacttgtgc aggattgata
    30061 ccagccgcag cagtgtagac catcaattta ccgatagcga tgtcaacacc gttcacaccc
    30121 cagtcaccca tacctaagat tccttctgca tctgtcacaa cgatcaaacg aatgtcgcgt
    30181 ccatctgctg cgtttttcaa acttgctttg atattttctg gttcgtcgat tgaaagaaat
    30241 gcagcatctt gtggtttcaa gaaaatttcg ttgtattgtt cgattgaatc cgcaacgaca
    30301 ggatcataaa cgacaggcat gaattcaact aaatgttcgc ccatcaattt ataaaacaat
    30361 gtccggttcg tattgaatag attcattaag aagatacgtt tttccaaatc ggtctgtttg
    30421 cttaagtatt gaccgtaagc ttggacagat tgttgttcca atgtttggac agtacttgga
    30481 agcataccag tgattccgta ttttgcccgt tcttcttttg taaaagctgt tcctttattt
    30541 agaaaaggat tatttaataa gtttagccct gtaagcatga atgattcctc ctaagtttta
    30601 atacaaacga agtataggag tcagatgagg atatagtcaa aaagagcagg tataataaat
    30661 tttatttata tgagtaatag agtcaaaaga aatcaaaata gcggtatttg ttctttttat
    30721 atcaacaatc cttgcttgat tactatgcgt atctcttata tgctaaagaa agaaaagtag
    30781 aagaataaag gatatgttag atagataaat agaatctctg aagaataaat cacgtgatcg
    30841 tgacgataaa atacacttac gtcaggtgat aaaaaataca taagtagaaa actaagagga
    30901 ggaacagaaa atgaaagaag ttcaattgat cagaaaaagc gaactatcag aaggtggttg
    30961 caatgcatgc ggcgtagtag aagcaacaag ctatacatta aagcttggtg ccaacaaagc
    31021 gataatctct gagttgacag taggcggtct tgtggacagt ctggcgcttg ctgaaggttt
    31081 tattggagaa gatatttatg agatgttttc agaaatccgt cagttaaaaa aaggggaaaa
    31141 ctgcatagag gttcatcatg agagcccgaa tgtgagattc aaacgtggtg ataatgagat
    31201 gatcttcaat aatcatgtgt ccgatcacac cgagctatat ggaatcgtga atcaaatact
    31261 aactgagctt tttggattgg gaccttatgc gttcaaggaa gaaaacggta atccaaaatt
    31321 aaacgaagaa tggcaagaaa cgattgaaat acaaagaaac aacccgcact tatttcaata
    31381 ggcatccaca gtatttcgta aaagggactt tgacacgact tttgtcaaag tcccatcgtt
    31441 ttttatctat ttgtgttcta aacatttctt tcagagtagt aacaactgga atgactgaaa
    31501 tgtaaacaga ttatttgcct gtatcaatac ggaagagtaa tataaagtgt gtaaacgcta
    31561 tcatattatt cattcaattt tttatagagg gaaggcattg aaatggaaaa aacgtatcaa
    31621 gcaggtacaa atgaaggaat agtggatttt atcaatatgg aagatttaga aatagccgct
    31681 tcacaagtga ttccagctgg tggttatggt tacatcagca gtggtgctgg cgacttattt
    31741 acttatcaag aaaatgaacg ggcattcaat catcagctga ttattcctca tgtattaaaa
    31801 gatgtagaat tacccgatac aactacacat tttgacaaag aaacgctgac tgcaccaatc
    31861 atcatggcac cagttgctgc tcatggctta gctcatgtga aggcggagaa agcttctgca
    31921 aaaggtgtag cagattttgg gacgatctat acagcaagtt cttacgcttc ttgtacatta
    31981 gaagagataa gagaagcagg cggggagaaa gcaccgcaat ggtttcaatt ttatatgagc
    32041 aaagatgatg ggatcaattt ggatattctt gaagtggcca agcgtaatgg agcaaaagca
    32101 atcgtattga cagcggatgc aacagttggc ggaaaccgtg aaacagaccg cagaaatggg
    32161 tttaccttcc cactgccaat gccgatcgtt caggcttatc aatcaggagt aggccaaacg
    32221 atggatgctg tgtacaaatc ttccaaacag aagcttagtc caaaagatgt agaatttatt
    32281 gctgctcatt ctgatttgcc agtctatgtg aaaggtgtcc agtcagaaga agatgtctat
    32341 cgttcattag aatctggtgc tggcggaatc tgggtatcta atcatggtgg ccgtcaacta
    32401 gatggtggtc cagcagcgtt tgattcgttg caatatgtgg cagaagcagt agataaacgt
    32461 gtaccaatcg tgtttgacag cggtgtacga cgcggacagc atgttttcaa agcaatcgca
    32521 tcaggtgcag atcttgttgc tattggcaga cccgttattt atggattgtc tttaggagga
    32581 agtacaggag tccatcaagt gtttgatttc tttaaaacag aattggaaat ggtcatgcaa
    32641 ttagctggaa cacaaacagt agaagatatc aaaaaaataa aattgcgtga gaatcgtttt
    32701 atttaagaaa caaatgacga aaaaaatagg agtgcggcag atgatgccgt actcctattt
    32761 ttatagttca aggatgtcac taatagaata atttgaaacg aaaaaataaa attgaaatat
    32821 tttttcatac ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct
    32881 cttttataca aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc
    32941 atactgtctc tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa
    33001 catgattggg acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt
    33061 tgctgagggt caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt
    33121 ggagcctctt aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc
    33181 agcaactggc cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac
    33241 acacgagatt gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc
    33301 ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa
    33361 cgtgacagtt tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt
    33421 agattttgga acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag
    33481 tcaaactgta actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa
    33541 agatcaaggt ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt
    33601 gccgaaagca gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc
    33661 agtagcatag agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga
    33721 ttttttaccg cagtttttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg
    33781 tttgtttcgt ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca
    33841 aaggattcag caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa
    33901 gcaatcggtt attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt
    33961 atcagcaatt cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg
    34021 agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg
    34081 tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca
    34141 gaaacaactg tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta
    34201 gcgggtattc atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca
    34261 caaatcaaaa atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg
    34321 atagaaccgg atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact
    34381 gttcagcttc gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc
    34441 aagatctttt atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca
    34501 ccaaattcga attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat
    34561 taccgcgcag aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac
    34621 tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa
    34681 gaacaatctt tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg
    34741 attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt
    34801 gatttcgttg aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag
    34861 ctggcaagag ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct
    34921 aagtccaatt ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct
    34981 accgatttat aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat
    35041 ccgttttctt tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg
    35101 gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct
    35161 ccttcttttt tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag
    35221 ctgttggagc aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac
    35281 ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta
    35341 accaattgcc taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag
    35401 gtggcacggt ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg
    35461 tgaatatcac tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat
    35521 caagcagtga tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg
    35581 cccccatcaa ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg
    35641 ggatggggtc gtgggataca aatactctat tgacagatat tattgctatt attacgcgag
    35701 gcgaattcaa cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga
    35761 tgacagaaaa cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat
    35821 tacaaccttc tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata
    35881 ttatccaaga tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta
    35941 aattaactgg cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa
    36001 atacgtggaa tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg
    36061 ataccacaga atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga
    36121 cattgacgat ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc
    36181 gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc
    36241 cagcaagcac aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc
    36301 aaggcggatt gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa
    36361 cggttttacc aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat
    36421 cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca
    36481 ggctttattc gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa
    36541 cgatccaatc aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt
    36601 cagggcaata ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt
    36661 cttttgcgta caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag
    36721 tggctaattt tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg
    36781 agacaggaga tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca
    36841 cgttaacaaa atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa
    36901 atactactta tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca
    36961 ttcaacaagg agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc
    37021 ttacttacaa ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga
    37081 caaaggcagc tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc
    37141 cagttgtcca aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta
    37201 atgtaccaga tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt
    37261 atgaatcaaa aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc
    37321 cttggcaact gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa
    37381 gcggaacaaa attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa
    37441 acacgttgat ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg
    37501 ataccgatgg tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt
    37561 atcaaggcat ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt
    37621 gtatggatct gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg
    37681 ctgttaaaat cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa
    37741 aatgaacaac ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc
    37801 gttaataacg tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt
    37861 tatttacaag ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat
    37921 attttattaa tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg
    37981 cgctattcta caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg
    38041 cagaaacttg tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa
    38101 ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca
    38161 gaaagtcaaa ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa
    38221 aacaaaaaag aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag
    38281 tttagttttg ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg
    38341 aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga
    38401 ttgtttcaaa cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt
    38461 caatatgcct ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt
    38521 aactttacat tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac
    38581 aaagacgacg tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc
    38641 tctttttatg tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat
    38701 cacgctcatt ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa
    38761 cagcggttaa ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga
    38821 caaacatttg ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta
    38881 tttgctcgct atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa
    38941 attgcttatg atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag
    39001 gaacaattgc tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat
    39061 gatatcattt tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt
    39121 gtattcacta gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa
    39181 tgttatttag aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg
    39241 cttcccatga aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag
    39301 ttttactgat cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc
    39361 tttatttaaa aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc
    39421 aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac
    39481 atcagctcat cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa
    39541 tcgtagcgac aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct
    39601 acacctataa aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag
    39661 aagtagtgat cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata
    39721 aaggatacat tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata
    39781 aggatatgat caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa
    39841 atgaattctt gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt
    39901 ctcaaacaat aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat
    39961 tgttaaaaga aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc
    40021 agacaagtga tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa
    40081 ataaacggca tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag
    40141 cgctaacttt gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag
    40201 tcattataga ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg
    40261 aacaaaatac cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa
    40321 acaaaacagt aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac
    40381 agctgataag ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac
    40441 acaaaactac gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct
    40501 ctcgttttca ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca
    40561 atagagtaga taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata
    40621 aatattaatc aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag
    40681 cataatattc gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag
    40741 gaggaggagt taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc
    40801 gttagttgca ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc
    40861 ttatggctta gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt
    40921 gctttcgaaa gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga
    40981 agaagtcgaa ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa
    41041 agacagtaca gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt
    41101 catcatgccc aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga
    41161 tattagtttg aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag
    41221 tgacaagacg tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt
    41281 tctctataca accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga
    41341 aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt
    41401 agaacagatc aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt
    41461 gacttttgga tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt
    41521 catttatgta ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat
    41581 cacaggagga tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg
    41641 tattcttctt ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc
    41701 attccaaagc aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt
    41761 gttaggcgca ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg
    41821 ataagaagga ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa
    41881 gatggcgacc aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa
    41941 tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc
    42001 ggattgcaga cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa
    42061 aaagaaagcg aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc
    42121 aatctggttc cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat
    42181 gaacatcgtc aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc
    42241 aacaagtatc cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca
    42301 ttgtacaatg atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa
    42361 aaggcacgag aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg
    42421 atcatggtta ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa
    42481 gatggtgtat tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata
    42541 caacaaagat acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg
    42601 atatctcggc acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct
    42661 ctatcattat ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa
    42721 agatgttgga aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac
    42781 cttgacaaaa atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct
    42841 tcacgatctt gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa
    42901 gaaaaattat cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa
    42961 tgggatttca aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac
    43021 tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt
    43081 gaatggtgta ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga
    43141 agcaagatat gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat
    43201 acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc
    43261 caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat
    43321 ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat
    43381 gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta cagccccttt
    43441 tttgcttact taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt
    43501 caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt
    43561 ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca
    43621 gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa
    43681 tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta
    43741 taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga
    43801 caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata
    43861 tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg
    43921 gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa
    43981 gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc
    44041 atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg
    44101 cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa
    44161 tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct
    44221 tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag
    44281 aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa
    44341 aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga
    44401 ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg
    44461 tttggccggg cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt
    44521 gagcctgccg ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt
    44581 gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt
    44641 tgtgaaatca acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata
    44701 ttttgccgtt tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac
    44761 aagggactgt gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt
    44821 tttctgtatt ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct
    44881 ccaatagttt tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc
    44941 cagcttcagc tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag
    45001 cagcagcaac agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta
    45061 attgattttt ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga
    45121 tcaaaatcgc ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat
    45181 tgtgcaaagc gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt
    45241 ctggagataa gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga
    45301 tgatttggct atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa
    45361 gctgatcacg tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc
    45421 cgacctcatc actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg
    45481 tttttgctgg cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg
    45541 cattcgtacc ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt
    45601 tttttgcagc aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg
    45661 ctttcatttg actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct
    45721 cctcgttttt atacataaaa ctataccgca actcacgact tttcgttttc taagagattg
    45781 cgagacatag cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg
    45841 tggagaacgg tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga
    45901 ggaaatgatt cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac
    45961 atatactatt atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa
    46021 tgaacagtta gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat
    46081 cttgatggct gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct
    46141 ggaagaagaa acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg
    46201 tgtgaaacgc attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca
    46261 agcagatcat accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa
    46321 aggacaaatt aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta
    46381 aaaaagttag aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct
    46441 aattgcgttt ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg
    46501 atatgtattg tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga
    46561 attatacatt ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt
    46621 atttgccttt cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct
    46681 aattggctgg ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc
    46741 gatcttgaga caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt
    46801 tactatattt ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca
    46861 attcggcgag catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa
    46921 gatgtatgag ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt
    46981 tcgttttttg tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct
    47041 cttttttcat agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga
    47101 atttgaaaaa gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga
    47161 actggcagaa ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac
    47221 tgaggatgga ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa
    47281 tatccgtccc catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc
    47341 acaagtttcc agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc
    47401 tgacagtgaa atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc
    47461 tgttttgagc cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc
    47521 ttctcctgag ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga
    47581 acagttagat gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac
    47641 catgcgaaat gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg
    47701 tgtagtccac gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt
    47761 ggatttgaat aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat
    47821 tttaagtgaa ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta
    47881 tgtgattggt aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc
    47941 catcgtgccg ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact
    48001 gatcgatcag gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat
    48061 cgtcattaca ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt
    48121 acaactgatg gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat
    48181 ttttgaggaa gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac
    48241 cttttcttct catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct
    48301 ggtattattt gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat
    48361 cgcaatctta gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc
    48421 tgaattgaaa gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga
    48481 tgtggataca ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc
    48541 gtttgaaatc tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt
    48601 gatgaatgat gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa
    48661 aatggcagag accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca
    48721 cgatgatttg aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa
    48781 agcaaagaaa aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat
    48841 tgctgatatt cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt
    48901 gatcgatgca aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa
    48961 agtattgaag aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac
    49021 gacttacggc caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat
    49081 cggtatatta aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga
    49141 gccgacacaa cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca
    49201 attggacctt cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga
    49261 ttctgccatt ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc
    49321 acttcgcaag ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt
    49381 tgcaccggca aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact
    49441 aaaggtaagc agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta
    49501 aaaatggagg tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca
    49561 gataaaggac ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa
    49621 gcaccgattt tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa
    49681 atggatgtgg atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca
    49741 ttaatgttga aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa
    49801 caactacgtc aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg
    49861 tgggactgaa tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt
    49921 aatatttttt gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat
    49981 aataacttta attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt
    50041 agctttactt cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat
    50101 ttatgtcggc aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca
    50161 taatacgaaa acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga
    50221 atcgaatatc gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata
    50281 caacattatg ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata
    50341 tccccgtttg gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata
    50401 tcccgatgtc ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag
    50461 aaaatgcaaa cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct
    50521 gtgtccttat tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa
    50581 aaacttttta aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa
    50641 agctgctgaa aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat
    50701 tgagacaatc atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt
    50761 tggttatgcg attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa
    50821 gctaattgaa cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt
    50881 gacttacctc ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat
    50941 tcctgatacg atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc
    51001 aaaacgagga gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt
    51061 gaatgaacgg tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg
    51121 tttaggaaac gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa
    51181 tatcatgggg acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag
    51241 aaaagaatat cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat
    51301 gagagaagtc atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga
    51361 tttgatcttg atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa
    51421 tcaattagga atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa
    51481 tgagttgcta ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt
    51541 tttcttactc cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca
    51601 gctacgcagt aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa
    51661 acgaaaaaaa atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac
    51721 agaagaatta caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt
    51781 acatcaggaa tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt
    51841 acaacaaaaa gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct
    51901 aaggatatat gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt
    51961 taaaactttt gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa
    52021 aatcatgggg ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt
    52081 ggattttttg acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa
    52141 agattatgat gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat
    52201 tcaagaccaa ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc
    52261 aaaaatcgac ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa
    52321 atctttgtct aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc
    52381 taaattgatc attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct
    52441 aaaagatgga atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa
    52501 tatggataat gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt
    52561 tttgaatgga aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga
    52621 atctggttta agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca
    52681 tgaagacaat acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac
    52741 tcgtgcgact cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga
    52801 gattttcaaa ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagata
    52861 tctatttaaa aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca
    52921 tgctgggtat catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa
    52981 tcggcgtatt ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg
    53041 attttgagtt caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa
    53101 aaatcgatgc gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata
    53161 gcgaaaattc attaggacaa agtacacaac tactgataca acaacaactg actggcttgc
    53221 aatcgatgct tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc
    53281 aacaagcaag tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg
    53341 gagaagataa ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca
    53401 ttattatttt gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc
    53461 ggatcatgga agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa
    53521 caggtgtttt acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag
    53581 gatacagtca attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg
    53641 ataacttgat tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga
    53701 ttttttctgt tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa
    53761 aagcaatttt gccagtgact tatctatctc taggcggata catgcttgga ttgatactag
    53821 gagcttcaga tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt
    53881 catatattat gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt
    53941 ccttgttggt acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca
    54001 aatcgaatgt actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt
    54061 caattatgag gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg
    54121 gacatctatt gttcccagct tctttttgta tagacctatt ttatttattt tactatattg
    54181 gatatatatt cattgaaaat agtgtataac aactttataa tattgagtat accaattttt
    54241 ggaggcgttt taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag
    54301 gatgtcatca cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag
    54361 ttatttttga gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt
    54421 tcaggaaatc ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat
    54481 atcagccaat tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac
    54541 aaagaacaag ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca
    54601 gtcaaatata cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt
    54661 gaggaactct tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc
    54721 attgaacaac ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg
    54781 atccaaaaat ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact
    54841 caagcagcca aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga
    54901 aatcatgata ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt
    54961 ttacggattg cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat
    55021 ttacgtccat tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct
    55081 aaaaacagta aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca
    55141 ctttctatcc ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa
    55201 atggacgatc gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa
    55261 ggtcagctct atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa
    55321 aaattcttgg aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct
    55381 ttgagaatgc aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac
    55441 aatcagcatg tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca
    55501 tttgaattag accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata
    55561 actgaaagcg caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat
    55621 gcgtggagtg ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt
    55681 tattttctca cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga
    55741 gatgaagagt ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca
    55801 gcaatcatta atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca
    55861 aatggaaaag tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga
    55921 attgccggtt attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg
    55981 tttttgccta ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta
    56041 atcgaacagc aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag
    56101 cagcagatta cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc
    56161 cagtgaacat tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt
    56221 ccggataaac ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca
    56281 tgaaaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    56341 ctcttggtat aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    56401 aatgtgaaaa gccattttca caaaattttt cttaatactc gaaactaaac agctttttca
    56461 caaccccctc agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca
    56521 gatggatgta cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata
    56581 tcatctttgc tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat
    56641 aaaggtgaaa gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg
    56701 agaaggttgc ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg
    56761 gcagtgggct gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa
    56821 agtgtacctt ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt
    56881 tttctttgtt tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt
    56941 gtttccgtta gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca
    57001 cctgcaagaa taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg
    57061 actggcagaa taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt
    57121 gtatagctca cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca
    57181 atgccgatga ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt
    57241 aaaaaaaatt aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta
    57301 tctgtaaaga gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg
    57361 ttataataaa aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct
    57421 tcaaggggtg agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag
    57481 gtacatactt tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga
    57541 agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg
    57601 ctcaaacact gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa
    57661 cgatccaaac aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg
    57721 aaatacaaga attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag
    57781 caactttgaa agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa
    57841 gggtgaaaga aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa
    57901 agaaatcagg agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat
    57961 ctgtatcaaa atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt
    58021 attgataaag aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct
    58081 atccaattca tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa
    58141 gaactttcct tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg
    58201 aaagaagtcc ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta
    58261 gaagaagagg aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat
    58321 gcaagtaaga cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc
    58381 aaggaagaac tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa
    58441 caagtttatc tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct
    58501 gggaaatacg ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg
    58561 caagagacca agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg
    58621 ttaggtcagt gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc
    58681 gttgcagaac gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg
    58741 caaggttggg tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta
    58801 tcagaagaag atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa
    58861 gtccctacta aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg
    58921 tacagtctgc caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt
    58981 gtcttcttcg ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca
    59041 atactagctc aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt
    59101 gagattctct cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag
    59161 tcattaccaa aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat
    59221 gacgtcaata cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga
    59281 ttatttgtgg cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat
    59341 ggatttgcct ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg
    59401 ataaaaaata cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt
    59461 atcttgatcg ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg
    59521 tataatcttt acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg
    59581 gcattgggta tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg
    59641 ccgccagctg ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat
    59701 atgtttttga cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt
    59761 tttgggaagt tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa
    59821 tacgtcaaca tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg
    59881 atggattact taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca
    59941 gcgacgattt tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca
    60001 gcagcattga caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt
    60061 ccaggcacgc aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga
    60121 aatgacatgt cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt
    60181 acaggcttgt tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta
    60241 gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac
    60301 gcgattttag gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg
    60361 aaaagcagat gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg
    60421 aaagagcggc gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac
    60481 gtacgcaagt tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa
    60541 aaaaataccg tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg
    60601 cgaagcagga atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg
    60661 atgaatctga acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa
    60721 aagtagcatt cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg
    60781 aatggaacaa tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag
    60841 cgggttttct gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac
    60901 aagatatcca aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga
    60961 tcagtgtatg gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt
    61021 gtccaatgag acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat
    61081 attgaaatcc acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca
    61141 gaatctttta aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc
    61201 tgaaatcgtt tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa
    61261 agctgagatg acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt
    61321 agaaaccttg aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat
    61381 ggacagtata cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga
    61441 cgtgatttat gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata
    61501 tcaagaattg ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc
    61561 tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg
    61621 aagtatcgaa aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa
    61681 ttttctatta gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat
    61741 tgatctcgta aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc
    61801 acaaacccag gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga
    61861 aagcaaaaac cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat
    61921 cagaaagcgg gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt
    61981 ctgttttgcc attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg
    62041 ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc
    62101 aatgtgcagc attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta
    62161 tcgtattgat tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa
    62221 acgtagaaaa agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc
    62281 aaattggaaa aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg
    62341 ccagtatcca agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga
    62401 tgcgtggcga tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg
    62461 aaccggttcg ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac
    62521 aaatgtttga cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt
    62581 tcttagggcg cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac
    62641 caacagtaga agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa
    62701 caaaagttat aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa
    62761 tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg
    62821 acaaaaattt gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa
    62881 aattaaaccc ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag
    62941 tgacaaaagg aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc
    63001 agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc
    63061 gtggaaatga gatgacggat gttttaaatg aattccctga attgattgac cctaatacag
    63121 gagaatcgtt gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag
    63181 ctcgagaagc ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt
    63241 atgacgttgc tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga
    63301 gcggacgttt ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc
    63361 ttgcggaata ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag
    63421 gaagtataac cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca
    63481 cacaaaatac attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa
    63541 aaagacattt tccttcgatc gattggatac aaagctattc tctttattca acagaagtag
    63601 ggaagtatat ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc
    63661 gtattttgca ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc
    63721 tttcagataa tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc
    63781 agcaaaatgc ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt
    63841 tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt
    63901 taagtgagat catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata
    63961 tcccggaaac agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc
    64021 aaacgatcgt ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa
    64081 gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc
    64141 gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat
    64201 aaagcaatgg tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta
    64261 cgatttttag gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc
    64321 gatggtttgg gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat
    64381 attaacggag aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca
    64441 ggtatctctg caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt
    64501 tctggctctg gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta
    64561 ctcgattcat cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa
    64621 gctgaatttt tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt
    64681 atgaatttag caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact
    64741 gctgcagaat atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg
    64801 accaactatg cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga
    64861 agaggatatc ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga
    64921 atccgcggac taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat
    64981 aaaactcatc caatccctga cttgactggt tatattacag aaggacagat catcttgact
    65041 agagagcttt acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg
    65101 ttaaaagata agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat
    65161 cagctttttg cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt
    65221 gaatctgctt tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa
    65281 gaatatgtca atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg
    65341 tgggaactgc tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg
    65401 gacaaatatc tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc
    65461 cgcatggaat tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta
    65521 ttaaaagata agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac
    65581 cagctgcgtc aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca
    65641 aatgcttcga tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt
    65701 gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa
    65761 tatgatgaac agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa
    65821 ctggatcggt caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa
    65881 atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat
    65941 gcattagaat atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag
    66001 ctggaagaaa acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc
    66061 actgactaag tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa
    66121 taaagaaacg tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag
    66181 ttggcggcgg cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta
    66241 tcgatgcgct tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata
    66301 cagcagagca ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg
    66361 gtcttggttt tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc
    66421 tcagcacacg tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga
    66481 agctcatgct ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt
    66541 tatcttttgt atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc
    66601 atgcaatctc tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc
    66661 accagcaaaa tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag
    66721 gcttcatcgt atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt
    66781 caaaaatcgc attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt
    66841 tcacagagcg caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca
    66901 ccttttttat gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata
    66961 tgagccatgc aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt
    67021 cgacggccgg cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga
    67081 tgttgaaagg acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt
    67141 tgcgggcact tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt
    67201 tatcggtgac tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct
    67261 tctctgcgtt tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag
    67321 ggaaattgat cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct
    67381 tctctctgat gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg
    67441 tcatgattgg ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat
    67501 aaaataattt ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag
    67561 gcactacccg ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga
    67621 tttgattcct ttatttatca ataggttata attcatattt ttttattttt tcttttacga
    67681 tacttagtcc agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg
    67741 catctggttg cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa
    67801 gaaagtcatt ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt
    67861 aagggacacg attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc
    67921 tttttgtaag acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt
    67981 gagtgatgac ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt
    68041 ctatcttgct ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa
    68101 taaattttgt cattccagct aagtagattg tttggttagg taataaattt tggaaaggag
    68161 gaatgatcgt atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc
    68221 atagaccttc tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa
    68281 accctatagg attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac
    68341 attcctgaat caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct
    68401 ctaaatgata taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta
    68461 ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg
    68521 aaacaataga tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg
    68581 cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat
    68641 ttgggttatt actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag
    68701 aactaaccag accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg
    68761 actcaaagac attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt
    68821 tctcaattcc atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag
    68881 ctagctctct ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt
    68941 gttctaattg ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc
    69001 gccaagttaa cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt
    69061 gtataccata caattttacc attgattgta ttgattttag aagttaatct tttgtgagga
    69121 gggaattgtc tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag
    69181 aagcgattca agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa
    69241 atcctcgatt ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc
    69301 ctgcttattt gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata
    69361 atcatcagta tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg
    69421 gactacccaa agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat
    69481 ctgcgctggc aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag
    69541 ctggtcaaca agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag
    69601 tgtttgttta cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac
    69661 agtatcctaa aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg
    69721 aaatcatttg tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg
    69781 aagacgcaca tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat
    69841 cgttgatgga aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg
    69901 gggacctgct tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat
    69961 taggcgaatt agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt
    70021 tcgacgcagt tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat
    70081 tagagaaagg ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga
    70141 ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac
    70201 gatgcaaaaa attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa
    70261 ccaagaattt gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc
    70321 gacattagca acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa
    70381 attttctact caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga
    70441 ttttcctagt attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg
    70501 ggctaagatt aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa
    70561 aacggtggct aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg
    70621 agtcatcgtg acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa
    70681 attaacaatc aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg
    70741 ggcagaaaga atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga
    70801 tctagaatgt gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc
    70861 taaaggtacc tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt
    70921 ggcaacctta tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa
    70981 aagtttgcgt ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc
    71041 cccatactaa ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt
    71101 agtaagaagg cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt
    71161 tatgttgctc gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt
    71221 ctcctagatc atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc
    71281 caggaagtgt caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt
    71341 cttcaaaacc aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa
    71401 cacatgtgtg agccattttc atagattcat ctctcctttt ccttttactc aatcataaca
    71461 aaaagaagta aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg
    71521 gatagaaaat aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa
    71581 aaaagcaatt tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc
    71641 agcagattgc agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg
    71701 aaggagaaga gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg
    71761 tggcagaaat ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg
    71821 caacttatta ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag
    71881 aaccaactga aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat
    71941 tgaaacgtgg tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa
    72001 agaaagcgag tagatcagct ttctctcagt ttttttattt attacagtac atattttttg
    72061 attgcttcag ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg
    72121 acaagagaaa cggcatttcc cattgctact cctagaccag catattcaat catggataag
    72181 tcgttctcgt tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg
    72241 agattcgcaa ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc
    72301 gcacttttga ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca
    72361 tctaaaattt ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt
    72421 tcttcaggtg ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa
    72481 gcgctgatat ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg
    72541 ccgacacggc gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg
    72601 gcgatgattt tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa
    72661 tcattatctt gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg
    72721 ccagtgcata aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact
    72781 tcttttgtta cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt
    72841 ttgatagaca taatttcctc caatatattt taatttattt caatcaatgc gccattacgg
    72901 atatggctgg caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac
    72961 ggatcaacca tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat
    73021 gcagcaacaa gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc
    73081 tcgatttgtg tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag
    73141 ctagaattca cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg
    73201 agttcttgga ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta
    73261 cgattcaaaa aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga
    73321 gtaaagtaag tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt
    73381 ccgtcaagaa atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga
    73441 tacaactcgt gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca
    73501 ggatggaagt acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca
    73561 tgcatgccac tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt
    73621 cgggtaagat caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag
    73681 tccattcgat ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa
    73741 gttttttgga tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc
    73801 tgatacactt ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta
    73861 tggaagatat gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc
    73921 gcacataaag tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt
    73981 gaaaaatttc gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa
    74041 tgagtaaaac ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta
    74101 atgcgtcgta gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg
    74161 acatggatat agttatggac tggatcacga aaaacttttt ccataggcag catttgatat
    74221 ttataagggg ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat
    74281 ttggttgttc cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa
    74341 atcacttgca gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac
    74401 aaaaaggggt tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg
    74461 atagccattt gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg
    74521 cccacgtttt tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt
    74581 gatccgacgc tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc
    74641 aggacaataa gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt
    74701 agtcaatagg agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg
    74761 ggggaaaatc aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga
    74821 atttctgatc actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa
    74881 aggagttcga acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa
    74941 aaagatttgc tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga
    75001 aaagtcgttt ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc
    75061 tcctttgtat aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg
    75121 gaaaaaaggt tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac
    75181 aatgtagcta tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt
    75241 tcaatcaaat tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat
    75301 ttgaaagggc aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag
    75361 gaggagacgg gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag
    75421 ctgatccgtg caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa
    75481 acatcctatc gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat
    75541 gttagtttga aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg
    75601 ggaccggaaa aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag
    75661 aaaagtttga ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg
    75721 tcctgattgg aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa
    75781 gtagcacacg cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac
    75841 cagatccaaa actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt
    75901 tatactgact tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg
    75961 cgttcatggt atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag
    76021 gatgaagata ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat
    76081 gaagacgcga tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat
    76141 gacgatgatc tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct
    76201 gacttgcaag aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat
    76261 atcgaagaag atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa
    76321 gaattttctg actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga
    76381 cgatcagcct atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta
    76441 tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg
    76501 tacgtcatat taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa
    76561 aacggcagat cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa
    76621 aaatgttccg tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc
    76681 aagatagttt tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta
    76741 gtgcatggtt ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa
    76801 ggacgcctgt ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt
    76861 ccgttgcaga gctccatatg attttagata aaatagacag tccggaaaaa gagattctga
    76921 tcctctactt ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg
    76981 aaaacacagt taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat
    77041 gaaggagaga agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact
    77101 tataaagaac aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc
    77161 gaagagcaat catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta
    77221 tctttggcta tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc
    77281 tttgcagaag atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt
    77341 actgatcgat ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa
    77401 gaaaataaaa caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt
    77461 gaaaaagctg aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat
    77521 tatcagaaaa tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca
    77581 gcagcagata gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag
    77641 ctctcacttc cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc
    77701 aaacggcaga tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa
    77761 gatattcaag aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat
    77821 ttgaacaaag agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat
    77881 atgggtacac cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa
    77941 gattatttgg atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag
    78001 tacaagtaaa taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt
    78061 tagttatcag tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct
    78121 ccttgatcac cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca
    78181 aacaatcaga acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag
    78241 ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta
    78301 caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag
    78361 gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt
    78421 ttttcgtctt aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag
    78481 cagggcacga gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc
    78541 agtcgatgac ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa
    78601 cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc
    78661 agcgaccgat catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt
    78721 tgattttgca gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata
    78781 tctatcctga aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt
    78841 atcgcatcac gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg
    78901 gcagaaacga tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat
    78961 tttaataagt agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca
    79021 ggtacaacaa aaaactgtcc accaatttt
//
LOCUS       DAYYAJ010000002        62163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000002 DAYYAJ010000000
VERSION     DAYYAJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.002"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..1569
                     /locus_tag="ACHATZ_000085"
     CDS             97..1569
                     /locus_tag="ACHATZ_000085"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8402649.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            1581..2405
                     /gene="nadE"
                     /locus_tag="ACHATZ_000086"
     CDS             1581..2405
                     /gene="nadE"
                     /locus_tag="ACHATZ_000086"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8402650.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(2478..3368)
                     /locus_tag="ACHATZ_000087"
     CDS             complement(2478..3368)
                     /locus_tag="ACHATZ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8402651.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            3566..5083
                     /locus_tag="ACHATZ_000088"
     CDS             3566..5083
                     /locus_tag="ACHATZ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8402652.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5055..6344
                     /locus_tag="ACHATZ_000089"
     CDS             5055..6344
                     /locus_tag="ACHATZ_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8402653.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            6519..7415
                     /locus_tag="ACHATZ_000090"
     CDS             6519..7415
                     /locus_tag="ACHATZ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8402654.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            7429..8292
                     /locus_tag="ACHATZ_000091"
     CDS             7429..8292
                     /locus_tag="ACHATZ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8402655.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            8298..8861
                     /locus_tag="ACHATZ_000092"
     CDS             8298..8861
                     /locus_tag="ACHATZ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8402656.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            8883..10394
                     /locus_tag="ACHATZ_000093"
     CDS             8883..10394
                     /locus_tag="ACHATZ_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8402657.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            10406..11365
                     /locus_tag="ACHATZ_000094"
     CDS             10406..11365
                     /locus_tag="ACHATZ_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8402658.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            11451..12530
                     /locus_tag="ACHATZ_000095"
     CDS             11451..12530
                     /locus_tag="ACHATZ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8402659.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(12782..14182)
                     /locus_tag="ACHATZ_000096"
     CDS             complement(12782..14182)
                     /locus_tag="ACHATZ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8402660.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            14400..16061
                     /locus_tag="ACHATZ_000097"
     CDS             14400..16061
                     /locus_tag="ACHATZ_000097"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8402661.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16018..16716
                     /locus_tag="ACHATZ_000098"
     CDS             16018..16716
                     /locus_tag="ACHATZ_000098"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8402662.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            16732..18156
                     /gene="araA"
                     /locus_tag="ACHATZ_000099"
     CDS             16732..18156
                     /gene="araA"
                     /locus_tag="ACHATZ_000099"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8402663.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            18463..19623
                     /locus_tag="ACHATZ_000100"
     CDS             18463..19623
                     /locus_tag="ACHATZ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8402664.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(19600..19740)
                     /locus_tag="ACHATZ_000101"
     CDS             complement(19600..19740)
                     /locus_tag="ACHATZ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8402665.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            19741..21600
                     /locus_tag="ACHATZ_000102"
     CDS             19741..21600
                     /locus_tag="ACHATZ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8402666.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            21710..22144
                     /locus_tag="ACHATZ_000103"
     CDS             21710..22144
                     /locus_tag="ACHATZ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402667.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22403..22564)
                     /locus_tag="ACHATZ_000104"
     CDS             complement(22403..22564)
                     /locus_tag="ACHATZ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402668.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22583..23605)
                     /locus_tag="ACHATZ_000105"
     CDS             complement(22583..23605)
                     /locus_tag="ACHATZ_000105"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8402669.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            23733..24086
                     /locus_tag="ACHATZ_000106"
     CDS             23733..24086
                     /locus_tag="ACHATZ_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8402670.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            24098..25264
                     /locus_tag="ACHATZ_000107"
     CDS             24098..25264
                     /locus_tag="ACHATZ_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8402671.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(25356..25976)
                     /locus_tag="ACHATZ_000108"
     CDS             complement(25356..25976)
                     /locus_tag="ACHATZ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8402672.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(26168..26464)
                     /locus_tag="ACHATZ_000109"
     CDS             complement(26168..26464)
                     /locus_tag="ACHATZ_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8402673.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            26668..27318
                     /locus_tag="ACHATZ_000110"
     CDS             26668..27318
                     /locus_tag="ACHATZ_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8402674.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            27334..29061
                     /locus_tag="ACHATZ_000111"
     CDS             27334..29061
                     /locus_tag="ACHATZ_000111"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402675.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            29058..30950
                     /locus_tag="ACHATZ_000112"
     CDS             29058..30950
                     /locus_tag="ACHATZ_000112"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402676.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            31654..32523
                     /locus_tag="ACHATZ_000113"
     CDS             31654..32523
                     /locus_tag="ACHATZ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8402677.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            32732..33757
                     /locus_tag="ACHATZ_000114"
     CDS             32732..33757
                     /locus_tag="ACHATZ_000114"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8402678.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            33796..34746
                     /gene="manA"
                     /locus_tag="ACHATZ_000115"
     CDS             33796..34746
                     /gene="manA"
                     /locus_tag="ACHATZ_000115"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8402679.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            34840..36543
                     /locus_tag="ACHATZ_000116"
     CDS             34840..36543
                     /locus_tag="ACHATZ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8402680.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            36545..38578
                     /locus_tag="ACHATZ_000117"
     CDS             36545..38578
                     /locus_tag="ACHATZ_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8402681.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            38589..39029
                     /locus_tag="ACHATZ_000118"
     CDS             38589..39029
                     /locus_tag="ACHATZ_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8402682.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(39229..40356)
                     /locus_tag="ACHATZ_000119"
     CDS             complement(39229..40356)
                     /locus_tag="ACHATZ_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402683.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(40359..41036)
                     /locus_tag="ACHATZ_000120"
     CDS             complement(40359..41036)
                     /locus_tag="ACHATZ_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8402684.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            41300..43066
                     /locus_tag="ACHATZ_000121"
     CDS             41300..43066
                     /locus_tag="ACHATZ_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8402685.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            43081..43518
                     /locus_tag="ACHATZ_000122"
     CDS             43081..43518
                     /locus_tag="ACHATZ_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8402686.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            43531..44682
                     /locus_tag="ACHATZ_000123"
     CDS             43531..44682
                     /locus_tag="ACHATZ_000123"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8402687.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(44744..45442)
                     /locus_tag="ACHATZ_000124"
     CDS             complement(44744..45442)
                     /locus_tag="ACHATZ_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8402688.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(45435..46787)
                     /locus_tag="ACHATZ_000125"
     CDS             complement(45435..46787)
                     /locus_tag="ACHATZ_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8402689.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(47010..48410)
                     /locus_tag="ACHATZ_000126"
     CDS             complement(47010..48410)
                     /locus_tag="ACHATZ_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8402690.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            48571..49308
                     /locus_tag="ACHATZ_000127"
     CDS             48571..49308
                     /locus_tag="ACHATZ_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8402691.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            49433..49912
                     /locus_tag="ACHATZ_000128"
     CDS             49433..49912
                     /locus_tag="ACHATZ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8402692.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            49909..50676
                     /locus_tag="ACHATZ_000129"
     CDS             49909..50676
                     /locus_tag="ACHATZ_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8402693.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            50906..51475
                     /gene="lepB"
                     /locus_tag="ACHATZ_000130"
     CDS             50906..51475
                     /gene="lepB"
                     /locus_tag="ACHATZ_000130"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8402694.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(51579..52181)
                     /locus_tag="ACHATZ_000131"
     CDS             complement(51579..52181)
                     /locus_tag="ACHATZ_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8402695.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            52287..52517
                     /locus_tag="ACHATZ_000132"
     CDS             52287..52517
                     /locus_tag="ACHATZ_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402696.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            52508..52756
                     /locus_tag="ACHATZ_000133"
     CDS             52508..52756
                     /locus_tag="ACHATZ_000133"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8402697.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            52862..53446
                     /locus_tag="ACHATZ_000134"
     CDS             52862..53446
                     /locus_tag="ACHATZ_000134"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8402698.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            53449..54522
                     /gene="prfA"
                     /locus_tag="ACHATZ_000135"
     CDS             53449..54522
                     /gene="prfA"
                     /locus_tag="ACHATZ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8402699.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            54515..55354
                     /gene="prmC"
                     /locus_tag="ACHATZ_000136"
     CDS             54515..55354
                     /gene="prmC"
                     /locus_tag="ACHATZ_000136"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8402700.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            55347..55526
                     /locus_tag="ACHATZ_000137"
     CDS             55347..55526
                     /locus_tag="ACHATZ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402701.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            55548..56573
                     /locus_tag="ACHATZ_000138"
     CDS             55548..56573
                     /locus_tag="ACHATZ_000138"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8402702.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            56764..58008
                     /gene="glyA"
                     /locus_tag="ACHATZ_000139"
     CDS             56764..58008
                     /gene="glyA"
                     /locus_tag="ACHATZ_000139"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8402703.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            58161..59108
                     /locus_tag="ACHATZ_000140"
     CDS             58161..59108
                     /locus_tag="ACHATZ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8402704.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            59242..59871
                     /gene="upp"
                     /locus_tag="ACHATZ_000141"
     CDS             59242..59871
                     /gene="upp"
                     /locus_tag="ACHATZ_000141"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8402705.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(60010..60252)
                     /locus_tag="ACHATZ_000142"
     CDS             complement(60010..60252)
                     /locus_tag="ACHATZ_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402706.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            60373..60445
                     /locus_tag="ACHATZ_000143"
     tRNA            60373..60445
                     /locus_tag="ACHATZ_000143"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60406..60408,aa:Thr,seq:cgt)
     gene            complement(60564..61202)
                     /locus_tag="ACHATZ_000144"
     CDS             complement(60564..61202)
                     /locus_tag="ACHATZ_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8402707.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(61325..61957)
                     /locus_tag="ACHATZ_000145"
     CDS             complement(61325..61957)
                     /locus_tag="ACHATZ_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8402708.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        20660 a        10865 c        12797 g        17841 t
ORIGIN      
        1 gtgctttttc agaaaaaatg gaagatttct tttcctatta attttttttg ttataatggg
       61 taggaaatga aaacttgcca agaggagtaa tacaagatga ataatactta cccagacgac
      121 agtttgacgc tgcatacgga tctatatcaa atcaatatga tgcagacata ctgggaatta
      181 ggtcgagctg atttacatgc tgtttttgaa tgttactttc gagaaatgcc ttttaaccac
      241 ggatacgcgg tttttgcagg attggaacgg ttagtcaatt atttagagaa tctgacgttc
      301 aatgattccg atattgctta cttgcgtgga ttagaagtct atcctgaagg ttttttggag
      361 tatttacaaa attttgaatt taaagcaaca gttcgttctg ctcgtgaagg agaactagtt
      421 tttgcaaatg aaccattaat ccaagtggaa gggcctttag cgcactgcca attagtagaa
      481 acagcactgt tgaatatggt caatttccaa acgttgatcg cgacaaaagc agctcgtatc
      541 aaatctgtca tcggagaaga tccactattg gaatttggaa ctagacgggc acaagaactg
      601 gatgcagcag tctggggaac tagagcggca tatatcggcg gagcagatgc aacaagcaat
      661 gtacgtgctg ggaagatatt tgggatacca gctagcggga cccatgctca ttcgttagtc
      721 caatcttatg gaaatgatta tgaagcattt atggcctacg ctaaaaccca taaagactgc
      781 gtgttcttag tcgacactta tgatacgttg aaatcaggtg tgccaagtgc gattcgcgtg
      841 gcgaaagaat taggtgacaa aatcaacttc caaggcgtac gcatcgacag tggagatatg
      901 gcctatatat ctaagcgtgt tcgggaacaa ttagatgcag ccggatttac ggaagcaaaa
      961 atctatgctt cgaatgactt ggatgaagca acgattttga atttaaagat gcaaaaagct
     1021 aaaattgatg tatggggtgt tgggacaaaa ttgatcacag cctatgatca gccagccctt
     1081 ggtgcagtat tcaagctagt atcgatcgaa gatgatgaag gaaaaatgat ggatacgatc
     1141 aagttatcaa gcaatgcaga aaaagtaact actccaggga aaaaacaagt gtggcgcatc
     1201 actcgaaatt ttgatgggaa atctgaagga gactacgtga cattatggga tgaagaccct
     1261 cgagaagaag aagctatctt catgttccat ccagtgcata cgtttatcaa taaaactgtt
     1321 cgagacttta cagctcgccc agttttacaa gatatcttta tcgaaggaaa aagagtttac
     1381 gaattacctg cactaaacga aatcaaagaa tatacaaaag aaaatctaga ttcactatgg
     1441 gaagagtata aacgagactt gaatccgcaa aaatacccag tcgatctgtc tactgaatgc
     1501 tggaaccata aaatggctat catggaacga atgaaaaaaa gcgtagcaga acttcataca
     1561 gaagcatagg aggagaataa atgagcctgc agacagaaat tatcaatgaa ttaggtgtca
     1621 aaccaacaat tgatccaaaa gaagaaatac gtaaaagtat tgactttatg aaagcatatt
     1681 tgaaaaaata tccgtttctt aaaacttttg tactaggtat cagcggcggc caagattcta
     1741 ctttagcagg ccgtttggcg caactaacga tggaagaaat gcgtgaagaa acaaaagatg
     1801 aaaactatca atttatcgct gttcgtcttc cttatggtga acaagcagat gaagaagatg
     1861 caaaagctgc tcttgacttt atccagcccg atgttagtct gcgagtaaat atcaaaccag
     1921 ctgttgatgc acaagtacaa gtattaagcg aagcaggtgt gggaatcagc gattttaaca
     1981 aaggaaatat caaagcacgt caacgtatga ttacgcaata tgccgtagct ggagaaagag
     2041 caggagccgt attagggaca gatcatgctg cagaaaacat tactggattc ttcacgaaat
     2101 ttggtgatgg tggagccgac attttgccat tattccgttt ggacaaacga caagggaaac
     2161 aattgcttca agcattgaat gcaccggaaa aattatacac aaaaattccg actgcagatc
     2221 ttgaagatgg gaaaccaatg attgcagatg aagtcgcact aggtgtcaca tataatgaaa
     2281 ttgatgatta tctagaagga aaagaagttc ccgctcaagc acaagaaaaa atcgaagcat
     2341 ggtggaacaa gacacaacat aaacgacatt tgccaatttc cgttttggat gatttctgga
     2401 aataacaata aagcaaaaac agccgtccct tcatagggat tggctgtttt tgttttataa
     2461 aatagaaaag aaaaatatca gctgtagtaa acttttaatt tgatatcctg atcgtaatta
     2521 ccgaatcctt taccaaataa agtacagccc cccacatgtt tggcatcgtc tttcacttcg
     2581 attcgcaaat gccaagtgtc ttgctcttta gggatgtcgt tgatcgtgat gtttgacaag
     2641 ggttctccat tcatattcgt caagtgtttg gtaattcgga tcgtcttcag caagccgtat
     2701 tgattaagat tatctggata ccaatcaggc gtatatttac cgcggatatc agcaaaatct
     2761 ccagggctcg tccatgttcc tagctccaca ccatttaggc tgaaagtgat atctgaaggc
     2821 caattgtcat ttgaaaaagg aaattcagaa gaaatttcca cagaaacttc caacatctcc
     2881 aatgtatcct ctgtatttaa aaaattaggt gcctgatact cgacaaatcc gctggtaaac
     2941 cagagaatgc gggcatccat ccttcgagga tccataaaat agcgtggttc atccactttc
     3001 ccgataaaat catggatcgt agctaaaccg catgttggtt caattgcata atttgtatag
     3061 tgaccaatag gtatcgatgt ttctttcgta tcaaaagcat tgaatatttt ttctggaaaa
     3121 ctgatatcga ttttatcgac acgaaggctc gaaatttttt gttgtcctac tttttctgtt
     3181 ttgatgatgt ttgcttcctc tagctttttc acgtgcatcg tcgtgatagc actgctcact
     3241 ccaagttctt ttgccaaatc tttgacattc atcttttttt tggaaagtag ctggatgatc
     3301 ttgatccttg ttttactagc taatgcttca tataccggta aggattcttc gtttatttgt
     3361 agttgcatat atatcacctc ttaaaaggtt tttcttatat attaacatat ttgttaataa
     3421 aagttatatt aaattatttg ttttaatcat tattaatatt ttggttaatt aaaatcgata
     3481 aaaaataagt aagtttaaca tttatattat tgacaacgct ttcaacgagg tgtattattc
     3541 atgtataaat taaaggaggt cagttatgga agccacaatg ttgattagta gtgtcaagcg
     3601 tgtaggcaaa gttgatccgc ggttgtacag ttcatttatt gaacatatgg gacgagcagt
     3661 atatgaaggg atttatcagc cagatcatcc atcagctaat aaaagtggat ttcgccaaga
     3721 cgtcattcac ttggtaaaag aattgaacat tcccatgatt agatatcctg gtggaaattt
     3781 tgtgtcagga tttcgttggg aagacagtat tgggcccata gcaaatagac ctagaaggtt
     3841 ggatttagct tggcggacaa ttgaaacgaa tgaagttggg attcatgaat tttataactg
     3901 gtgtcagcat gtgggggccg aaatcaatat ggctgtcaat ctgggtactc gtggagtaga
     3961 tgcagctcgt aatttagtcg aatattgtaa ttttcctaaa ggaacttatt ggagtgattt
     4021 aaggcgttcg aatggtcaag agagcccttt tggaattaag acatggtgtt tagggaatga
     4081 aatggacggt ccttggcaga ttggacataa aacagcggaa gaatatggac ggttagcaga
     4141 ggaaacggct aaggcgatga aactcgtaga tgattcaatt gaggtggttt tatgcggaag
     4201 ttcgaatcgg caaatgccta catttggtga ttgggagtta acagttcttg atcaagcgta
     4261 cgatcaaata gattatttgt ctctgcatca atattatggc aaccaaaaaa atgatctttc
     4321 tcattatctt gcacgttcct tagacatgga tcggttcatt tctgaagtga cagcgatgtg
     4381 cgatacagtc aaggcgaaga aacatagtaa gaagcagatc aacctgtcct ttgatgaatg
     4441 gaatatttgg tatcattcag cggaacaaga tgagcaagca gcgccttggc agattgctcc
     4501 tcctttacta gaagataact ataattttga agatgcatta ctattaggct gtctattaat
     4561 cactttattg aaacatgcag atagagtaaa gattgcttgt cttgctcaat tggtaaatgt
     4621 tattgcacca atcaggacag aaacaaacgg agaagcttgg agacagacga tctattatcc
     4681 atttatgcaa gctgctgtgt atggacaagg agaggtgtta agcccacaaa ttcgttcagc
     4741 aaactatgcg acggaagatt ttgaagaagt gccgtattta gaaagtatag ccgtaataaa
     4801 tgacaaagaa cttgtcatat ttgcagttaa ccgtggagaa gaagaaatga atctaactgt
     4861 tcacttgtct gaattatcat tagagggagt gattgatttt tctgaaatga gtggatttga
     4921 tacaaaagca actaatcttc ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt
     4981 aaaagctgaa gatgggaaaa tcaattgttt attgaaaccg ctatcttgga atgtggtcag
     5041 atgtcgttta tcagatgaag gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct
     5101 ctttgttggt agctgcaaca ggtattttag gagcttgcgg tggcggaagt tcagcagaat
     5161 ctgattctga taaagaagtg actttgactt tttggaatgg gtttactgca tctgatggtg
     5221 aaatattgca agaaatcgtt aatgaattta ataaaacaaa tgataaaaac atcaaaattg
     5281 aaatggatat catgacttgg gccaatttga atgaaaaact tcctacagcg atttcttcta
     5341 aaaaagcacc tgactttatt gcattaaatt atccagattt tgcacagtat gtagaaaacg
     5401 gagcggtaca accattagac gatttctggg aatatgatgg agtagataaa tctgatttta
     5461 aagaaacggc tattgaatta ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg
     5521 tacaaggcat gtatatgtat tggaataatg atttgtttaa acaagctgga ttagatactg
     5581 aacaaccacc tactacttgg gaagaattga cagaaatggc accaaaatta actgatagta
     5641 gtaaaaatgt atcaggtttt gtcttcaata aggatggaac tgcaccgtta tataactgga
     5701 tgttagcaaa tggcggtaaa ttagtgaacg atgattatac taagtctgaa tttgcgtctc
     5761 ctgagaacct taaaacgtta aaagcgatcc aaaaaatgat ccatgtagac aagactggtc
     5821 cagaatcgat ttctggcgcc gaaatggaca atttaatgaa tgctggtcaa ctagccattg
     5881 aaatcaatgg gccatggtta aataacggat tgaaagcaaa cgagattgat tataatgtga
     5941 ctaccttacc acaagggagt gattcagaca aaaaaacagc tattttagat ggcgtaggat
     6001 atgccattcc ggcaagtaca gataataata aaaaagaagc tgtctatgag tttttgaaat
     6061 attggaatac aactgaaatt ggtaaaaaat ggagtatcga aaatggtttt cctgcctact
     6121 tggattcagt agcaaaagat gaagaagtaa aaaatgatcc tattgtatct gaattatcga
     6181 aacaaatgga ctatgcagag ccatatttac caggttttac ccaaatctct gcgatcaata
     6241 acgatatcat caatccactg attgaaaaat tattagcagg agaagatcct gagacactca
     6301 tgaacgatgc agatgatgcc atcaatcaat tactgcaaaa atagtacaga actttataca
     6361 ggatagactg ttggcaagat acagatatct gaatgatcga cctagtattt gcagataggt
     6421 gttattgcaa aaatcacagc gattgttcgg atatcttatt cagattcgtt taaaccaaga
     6481 ttgtatattt tatcaaaatg aaagatgagg tggactatat gaacggattc accaaatatt
     6541 ggaatcgtcc tgatcgtgct gcgtatttgt ttttaactcc atctttttta gttttattat
     6601 tatttactgt cataccgctt ttaggaacat ttgctattag ttttacagat ttgaatattt
     6661 tctttacatc aagaaatttt gtaagttttg aaaatttcac acgtatattt agtgatgaac
     6721 gagcagttaa ttcgttgatt cattctttat atttcaccgt actcgaaacg ccaacacaaa
     6781 ttatcgttgg tttattagca gcagtagcat tatctaaaaa taccagattc aatcgttttt
     6841 gtcgttcgac attttacatt ccagttattt gttcattgac gtcaattgcg attatttttt
     6901 ctatgttgct tgatccgaat gtaggggcga ttccttactt gttttcacaa gtcggcttgc
     6961 cgatccctca attttttcgc gatccaaccc ttgctatgcc gacggtagcc atcatgactg
     7021 tttggaagaa ttttggtgtt actatgacga ttctcttgac tgctattcag ggaatctctc
     7081 cctctttgta tgaatctgct caaatggatg gtgcaacaaa tcgacagcag ttttttaaca
     7141 ttacgctacc tcagatcgtt ccctccttag gtttttgtat tttaacgaat ctgattggtt
     7201 cgatgcaagt gtttgaccaa gtatatgttg caaccgctgg tggcccacaa tttaagacag
     7261 aaacagctgt tcaatatatt tacagtcgag gatttacagc accttatgaa ttgggctatg
     7321 catcagcact gtcttcggtt ttgtttatca ttgttgcaac tatagctatc tccactaatg
     7381 tgtacatgtc acggaaagaa aaacaaatga aataggaggg aaacctgtat ggcaccagaa
     7441 agctcgatta cagttactgt gaatagtcga aaagcagtta gtggtaaaaa aatcattcga
     7501 acgattagta tgctagttat attgttggtt attgcgtttc cttttttgtg gctgattatt
     7561 tcttctttta aacacgaaaa agacattatc tcatttccgc ctagaatatt tgcagatagc
     7621 tataccctag ataactatat taaggtttgg acaacgatcc ctttattaga ttacatcaaa
     7681 aatacagtga tttttgctgg agggacagtg atcacttctg tattctttga ttctcttgcg
     7741 gggtatgcat ttgctcgtat gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg
     7801 ctgacaatga tgatcccatt ccaagtcttc atgattcctc tatttattga agtcaatttg
     7861 cttggcatgc ttgatactta tgcaggtttg atcattcctc gtatgacaac tgcatttggt
     7921 atcttcatga tgcgttcctt ttttattact ttaccagatt ctttggaaga ggcagcacgc
     7981 attgatggat taagtgagtt taatattttt ttgaaaatca tgttgccatt gagcaaacct
     8041 actcttttat cattagggat tttcacgctg atgaatagtt ggaatgactt gctttatcca
     8101 ttgatattga caagtagttc aaaaatgaga actttaccag cagggttagc tttatttaca
     8161 gggcagaaca tttcttttta tggacctgta atggctggaa cggttatttc aatgctgcca
     8221 ttactagtgg tttatatttt tgcacagaaa tattttgttc aaggaacagc tatgtctgga
     8281 atgaaagagt gaggaagatg aaaatagttg agactaaact ttatggacag ttgattgtgg
     8341 tttctgatta catcttatta ggtattctct gggtaatcgt gtgtttgcct gttattacta
     8401 tcgtgcctgc tacatgtgct gttttttatg taatggcgca atggaaaggt gagggatcag
     8461 gaaatatcat ttattttttc tttcaaggaa taaagcaaca ttttggtaag aacttgttcc
     8521 ttagtctgtt aacgatcatt atttattttc ttgttaatca attattagaa gaacagacaa
     8581 tcgtactttc aattagtgga tatgtagttt tacttattta ttccatgttt cttttatcct
     8641 ggatatatgt gatttctgaa aacaaagata tcacattttt acaaattttt gaacaaagtg
     8701 catggaaagt attttttgat tttttgagaa atattctttg ttgcgtattg ttattaggat
     8761 ttgtactgct catatttctt ttccctcctt ttatttttat ctttgctggt ggcgtatgga
     8821 aacttgtatc tattatcttg actataggaa atgaaaggta gggaaagctg ttggacgaag
     8881 aagtgaaagt aaaaatcaga gaggatttta cggtaggaga gatcagttct cggctgtttg
     8941 gctcatttgt tgagcatatg ggaagtgtcg tctatacagg gatttatgaa ccgggccatg
     9001 aaacagctga tggtgacggg tttagacaag atgtcctctc ttatgtaaag gaattagaat
     9061 taggtgtcat tcgctatcct ggaggaaact ttacatctgg ttatgattgg gtggatactg
     9121 taggtaggaa agaacaacga ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa
     9181 attcttttgg actccacgaa ttttttaaat ggatcagtca tgtacaagcg aaaccgatat
     9241 tcacggtgaa tttaggaaca aaaggaattg atgaagcaaa aaatttagta gaatattgta
     9301 attttccaaa aggaaccttc tggagtgatt taagaataaa aaatgggcaa aaaaagcctt
     9361 ttggcgttaa attatggtgt ttagggaatg agctagacgg tccatggcaa gtgggggcta
     9421 agagtgctta tgaatatggt aggttagcaa atgaagcctc aaaagtcatg aagttagtgg
     9481 atgattcaat tgaaacaatc ttagttggaa gctctacgcc acgtttacca tcgtaccctg
     9541 aatgggatcg tatcgcatta gaagaagctt atgagaatgt agattattta gctttgcata
     9601 actatattga taagtatgat gaagaagact tgactaaacc tcctaaaaat gagcgtgatg
     9661 atacagcgac ataccttgca aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg
     9721 ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat ctagcgtttg
     9781 atgaatggaa tgtacatgcc caaccggaaa aaagacatag ggattttgaa gtcggtagtc
     9841 ccatcgattg gtgttacttt actatggaag atacattatt atttggttca ttagggctgg
     9901 ctattttacg acacgcagat cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa
     9961 tacctttaat actgacagat gctaaaggac aagcatggag gaatccaact tattatgtga
    10021 tgcaacactt atcgaaatat gctaaaggtc aagtattgaa acaacaaatc atttgccctg
    10081 tttatgattg tgaaaagata aaaaatgttc cttatgtgga cagtgtgatc gtagataacg
    10141 gcaatcagct gactattttc ttgatcaatc gcacaaatga acaacaaaag ataacgattg
    10201 aacatgattt caatttgcta gatcaaggag agcactacac attaaccagt agcgatctgt
    10261 tggacaaaaa tacacgtgaa gaaccagaaa aaataaaacc tgaaataaac tatttttcaa
    10321 tagaaaaaca tgacgaatta aatgtaggga tagaaaaata ctcttggaat gtaattagaa
    10381 taaagaaaat gtgaggaaat ttgttatgat gaagacactt tataataatc ctatcgttcg
    10441 gcaacgggca gatccttggg tatataaaca tacagatggt tattattatt ttacaggatc
    10501 ggttccaggc taccaagtga ttgaattaag aagggcaaag tcactgaatg aattagaaca
    10561 tgctgcaagc ctgattgtat ggcaggctca tgaagaagga ccgatgagtg aattgatctg
    10621 ggcacctgaa gtccattata tcaatggaaa atggtatatt tactttgctg caagcaatca
    10681 taaaactata cgtgatgaat ctcatcatca ccgcatgttc gtacttgaaa atcagcatat
    10741 agatccaatg aatacagatt ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt
    10801 cagtttagac ggaacaactt tctcttctca aggaaagcta tattatgtgt gggcccaaca
    10861 agatcctgaa attactggga attctaatct ttatctatcc gaaatggata atccttggac
    10921 gttaaagggg aagcagatcc tcctttctat acctgagtat gaatgggaaa aacggggttt
    10981 ttcagtaaat gaaggtccag cagtccttac tcgcaatggg aaaatattca ttacgtattc
    11041 tggtagtgca acagatgaaa actatgccat gggcctgctg tgggctgatg agcacaagaa
    11101 tcttttatta aaagaatcat ggcataaatt atcggagcca gtattcgttt cttctaaaaa
    11161 aaataagcaa tttggtcctg gtcacaactc gtttacggtt tcagaagacg ggtgctttga
    11221 tattttgatc tatcatgctc gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc
    11281 taatcggcac gcgaacgcgc aatgctttac gtggaatgag caaggatttc cagaatttgg
    11341 tgaacctgtt ccgtatgcct tatgagcaca agaaaccttg atttttgctt gaataaacct
    11401 tgcagactcg ttatgatata tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat
    11461 atcaagatat tgcagaagag ataaaacaaa agatccttcg tgaggaatac gaagtaaata
    11521 caaccattcc ctcagaattc aagctgcaag aaatatatga cgtcagcaga catacgattc
    11581 ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca
    11641 catatgtaga tgacagctat aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg
    11701 gtgtcatcac cacatatctc tctgattata tttttccgtc gattatccga ggaatcgaaa
    11761 atgaattacg agaatctggg tattcattgc ttttagccag tacgaataac gatcacgaat
    11821 tggagaaaga gtgtttgaag aaaatgattg cttatggggt agatggtttg atcgttgagc
    11881 ctacgaaaag taatcagtat aacccaaact tagcacttta tgttgctttg agagaacaag
    11941 gcattccgat ggtgatgatc aatgctgctt atgaagagtt aaatgcctct tatatttgtt
    12001 tagatgatgt gaaagtcggt tttatggcag cagaatattt gataaaaaaa ggacatcagc
    12061 gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa atacaggatg aaaggtttta
    12121 tcagcgcgtg tgaacagtat aatgtgcgtt ttttgacgga agatatcatt actttcacca
    12181 cggaaacacg agaaacaatc gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt
    12241 tagatggcat catttgctat aacgatcaaa tagctagttt aatgctagat aagttgacag
    12301 aaaatggcta tcatattcca gatgacctat cgatcgtagg aacagacgat tcttctttaa
    12361 gccatttagg gaataaaaaa ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag
    12421 atgccgcacg gtggatcgtc acaacgattg aaaaaggaca ggcacagcca aatattctct
    12481 atgaacctgt actagttgaa cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt
    12541 tttaccgcct cttattgctc ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca
    12601 tcgctcaagg ctgaacgttt ctttcaccac ctctccacta aacggtgttc tcctaccaac
    12661 tggtcacaag tcacaatatt catcaaacat gagaagctgt tttttgaaat tgttccttgt
    12721 gactggaaac taagcggttc cttcttattt cttgaagctg accacttctg tcacaacctc
    12781 tttaagaatg atggatagaa atacgtttag gataagtttt tttagatgca ctttgttcga
    12841 tttcctctaa agatttccca cgagtttctg gtacgatctc ttgaataaat aaaaccccta
    12901 aaaagcagat gatcccaaaa atgccgaaaa cgatttcttg agacatagaa gcagtcatca
    12961 caggaaaaag caagccaact aggaaagaac caatccaatt aaaagaagag gctaggccag
    13021 atgctcgccc gcggacagct agtgggaata tttcgcctac gattacccat gtcagtggag
    13081 cccatgtgca agaataaaat gcgacataaa tacataaaaa caagagaatc aacaacgaat
    13141 ttgtatgagc atccagtaca gttcctaaaa cagctggtag gatgaaagaa agtcccatca
    13201 cgctaccgcc tattttcaat aaagttcgcc gattgaactt gtctgcaata accaaaaaga
    13261 gcaaagaacc agcaactaaa atgattcctt ggatgatcgg ccacatcaat gcatcgctcg
    13321 ctgcatgccc agtcgctttt tctacgatca gcggaatata gtaaaaaata gcattagctc
    13381 cttgaaattg ctggaatact gctacaccaa taccggcaat gactaaggaa cgatattttt
    13441 ctaaaaacaa agtatgccaa gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga
    13501 tttgttcgaa ttcctgttca gcttcttttg gtgtccgaat atagttcaat actctttttg
    13561 cctcttccaa gcggccagat tgcataagaa aacgcggtga ctctggtaga gctaaaacac
    13621 cgaaaaataa aatcagcgcg gggacagcag cgagccctaa catcacgcgc caagccatcg
    13681 tttcaggcaa cccttttagc aaataatctg caacatagga aaggagcatg ccagaagcaa
    13741 tcatcacttg attgatccct gacaatcttc cccttagtct agcaggagcc atttcagaca
    13801 tataagcagg aactagggca gatgcagcgc caacagctaa acctagaaga atccttgctg
    13861 caatcagaaa ataagaacca ttgtgagggg cagatgcaga aaggacagaa ccagccataa
    13921 aaataagtgc agaaagaaga atcatttttc gtcgtccaag cttatcagaa agaagaccag
    13981 ataaagagcc gccaaggatc gcacctaaca tgaccgaaga cgtgatccat cctatcaaag
    14041 aagctttacc tgccaaaccc caatcatgct gtaaaaaagg taaggcgccg gtcataacac
    14101 caatatcata tccaaataga atgccgccaa atgcgccaaa aaaataaata aacgatgagg
    14161 aaactttttt ttcttttttc ataagtattc tctccttcta gaatttcgag aaggcggttg
    14221 atcagatttt gtaagcaatt tcattttata acttatgtac gtacaaaaca agtgtttttt
    14281 attgtattct tgtaaataac tgcttgacat gtacgtacaa aagtatacaa taatcattgt
    14341 aagcgctact aaaaagacgg tttgatcagg tttgacgaga aggagggagt taaatcatta
    14401 tgaaggaagt aaatgcgcaa agtatgttag atgtcataga tggtcgtaca tctttaggga
    14461 ttgagtttgg atcaactagg atcaaagctg tattgattga ttcatctttt actcctattg
    14521 ccgctggtag ttatgagtgg gaaaacaaac tagaaaatgg tatctggacc tattcattag
    14581 aagatatttg gaaaggactg cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa
    14641 tctatggcga gaatcttacg acaatcgggt cgattgggtt cagtgcaatg atgcacggtt
    14701 atctagcatt tgatagcaag gaacagttac tagtaccatt tcgtacttgg cgaaatacaa
    14761 tcactgaaga agcagaacga atattaacgg aaaaatttca atacaatatt ccgcagagat
    14821 ggagtatcgc tcatttgtat caatctattt tgaataaaga agaacatgtg aaagaaatta
    14881 attttttgac aacgcttgca ggttatgtcc attggcagct tactggtgag aaagtactag
    14941 gcgtaggaga tgcatcaggc atgtttccta tcgatccttc aacgaaaacc taccatcaag
    15001 aaatgaaaaa tatttttggt cagttaatcg aaaaagcgca cgtagcgatt gacttggatc
    15061 acattttgcc taaagttcta gtagcaggag aagaagctgg caagttaagc gaatcagggg
    15121 cttatctgat cgaccctacg ggaaatttga gaccaggaat cccggtttgt ccgccagaag
    15181 gggatgcagg aaccggtatg gtcgctacga acagtgtagc caaagggaca gggaacattt
    15241 cggctggtac ttccgccttc gctatgatcg tattagaaaa agaactagaa aatgtccatc
    15301 cggaaatcga catggtcact acaccttcag gagaactagt aggcatggtt catacgaata
    15361 actgctcttc ggatatcaat gcttgggtca gtatttttga agaatttacc aattctttgg
    15421 gtatggagat aagcagagat aaattattca cggtattatt caacaaagcg ctagaaggcg
    15481 atacggattg cgggggatta ctttcttatg gttatttttc cggtgaaaac attacaggag
    15541 taaatgaagg aaggccatta tttgtacgta caccaggtag ccgttttgat ttagcgaatt
    15601 tcatgcggat ccatttagcc agtgcttttg gtgcaatgag aatcggaatg gatatattga
    15661 aatctgagaa tgtccaaatt gatcgtttag tggggcatgg ggggattttt aaaacaccag
    15721 aagttggaca gcggattcta gcatctgcta tggaggcacc agttacagtg atggacacag
    15781 ccggagaagg tggagcgtgg ggaatcgcgt tattagcagc ctatatgatg gatcgacatg
    15841 ggaagtcttt agaaaacttc ttggcagaag acgtgtttgg acaagaccaa ggaatcacca
    15901 tcacaccttc agcagaagaa atagcaggct atcagatatt tatgagacgt taccgtgagg
    15961 ggatcgacat tgaaaaaaca gcgattcatc aattgcaaga aaaaggagtg atagaaaatg
    16021 ctggaacaac tgaaagaaga agtataccaa gcaaatcttg acttgcctaa acacggactc
    16081 gtcaaatata catggggaaa tgtgagcgct tttgatcctg aaacacgata tttcgttatc
    16141 aaacctagcg gagtcagtta tgaagagatg acagcagacg acatggtcgt tgtggacttg
    16201 gacaatcata tcattgaagg taaactcaat ccctcttcgg atacgcctac tcatgctgtt
    16261 ttatatcgtt catttcctca aatcggagga atcgtccata ctcactcgac atgggcgacg
    16321 atctgggcac aagcaggatt ggatgttcct gcgatgggga cgacgcatgc agatactttt
    16381 tatggatcgg taccttgtgc tcgatttttg acacaacagg aaatcgacag cgggtacgaa
    16441 tatgaaacag gaaaagttat catagaaaca ttcaaagaac gcaaaattga tcccttagca
    16501 attccagggg tacttttaca cggacacggt ccatttactt gggggaaaga tgcgcaaagt
    16561 gcggtaatga acgcagtcgt actagatgaa gtatgcaaga tgaatttatt taccagacag
    16621 ctcaattcat tttcagaaga gctgcctcaa cggattttag ataaacatta tttacgaaaa
    16681 cacggaaaaa atgcgtatta cggacaaaac aaataaaagg agtggctaat tatgttgaat
    16741 attggagaaa aagaattttg gtttgttgta ggatcacagc atttatatgg ggaagaagca
    16801 ctgcgagaag taaagaaaca tgcacaagaa atggtcgatg aactgaatga aaatggccaa
    16861 ttgccttatc caatccgcct tcaagaatta gcagttactg ctgatacgat cacaaaaatc
    16921 atgaaagagg tcaattatcg agaagaagta gcaggtgtga tcacttggat gcatactttc
    16981 tcaccagcaa aaatgtggat ccgcgggaca aagcttctac aaaaaccatt gcttcattta
    17041 gctacgcaat ataacgaaag cattccttgg aaaacgatcg atatggattt catgaattta
    17101 aatcaatccg ctcatggtga ccgcgaatat gggttcatca atgctcgatt aaataaacaa
    17161 aataaaatcg tcgttggcta ctggaaacga cctgagatcc aaaaagagat tgctgattgg
    17221 atggatgttg cagtagccta taatgaaagt tttggaataa aagtggctcg ttttggtgac
    17281 aacatgagaa acgtgggtgt cacagaagga gacaaagtgg aagcacagat tcaatttggt
    17341 tggactgttg attattttgg tatcggagat cttgtacaag taatcgatcg cgtatcagac
    17401 gaagaagtcg atcaattatt tgaagagtac aaggaattgt ataccttcga ctatggtgat
    17461 tatgaagaaa agacatggga ggaacatgta aaagtacaag ctcagcagga aatcggtatt
    17521 cgccgtttct tagaagaagg aggctataac gcttttacga ctaattttga agacctgtat
    17581 ggaatgaaac aactacctgg actagctgta caacgtttga tggcagaagg atacggtttt
    17641 gctggggaag gcgactggaa aactgcagcg attgatcggt tgctgaaaat catggctcga
    17701 gggaaagata caggattcat ggaagattat acttacgagc tagctagtgg ccaagaagca
    17761 atcttggaat cccatatgat ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc
    17821 gttgtttctc ctttatcaat gggagataga gaagatccgg cgcgtcttgt atttgatggg
    17881 aaagcaggag aaggggtagt cgtatcgatg gcagatttcg gtacgcatta taaactgctg
    17941 atcaatgaag tagaagcctt tgaaccaacg actgaagcac ctaatttacc tgttgcacgt
    18001 gtgttatgga aaactaaacc gaatttccat gaaggtgtcc attcatggat tcaggcaggt
    18061 ggcggtcatc ataccgttgt ctcgctaaat ctaacaacag atcaaattga aacgtgggca
    18121 aaattagttg agttggaaac agtggttatt agataaaaag aggttgtgaa aaagccactt
    18181 agttttaaga aataagaaga gattgacgaa atttgctttt caactttttt gaaatttagt
    18241 cttattatca atgtcagcta acggaacacc atttatcaag atatctcacc ctcaatattt
    18301 tgctctcgaa aataaggctt atggcagtaa cttgtcatag ccttattttt atcactcagc
    18361 ctactctgct ctaactgctc tttttgcttc taggaagtca aatggtaaac agttgtctaa
    18421 ttgcagaaat ttcgagatac ttgttagtaa ggagggggtt ttatgtatca cgttcatttg
    18481 attgatgcta ttccagaaca agagatagaa aagttacaag gggaatattt caaaataata
    18541 gatgatttgc cagccgaagg aatagttatt cgtagtacga aagtgaaaga tgaatggctt
    18601 acaccagatt tgctagcaat ttcaagagct ggtgttgggg tgaacacgat caacgttgaa
    18661 aaagcttctg aaaacgggac gatcgtcatg aatacaccag gtgtaaatgc taatgcagta
    18721 aaagagcttg ttttgtgctg tttgctctta tcttcccgtc cgataatcga agcaagtcgt
    18781 atggtacaaa cactaacagg tcctaatatt ttagaacagg cagaaaacaa aagaagtgct
    18841 tatgtgggac gagaattgca aggcaaaacc atcggtcttc ttggtcttgg agcgataggc
    18901 acaaaagtag cgctaagttg ctatagctta gggatggatg tcttgggtta ttctatccgc
    18961 gatgcccagt tggattatgt tcggcaagca gatttagaaa cagttctttc tacctcggat
    19021 tacatcgtag tcatgctgcc gttgacagaa gatacaaaag gattgatcga tcaggcgaat
    19081 atagaaaaaa tgaaaaaaga tgctgtactt atcaatgtag gaagaagtga aattgttgat
    19141 aaatatgctg ttatgcaagc attggaaaaa caacatctgg ctaaatactt gactgatttt
    19201 ccagaagaag agttcttaga aaatgaccgt atacttatgt tgcctcatct agggggatcg
    19261 actcaggaag ctttggcaga cagcggacgt ttggcagttg aagccttaaa agattatctt
    19321 ttgttcggta cggttagaga agcggtcaat tatccatccg cacgaatgtt tttccaagcg
    19381 ccgtttcgat tcactatatt ttatcaaaag aaaaccaata tattagcaga gatattttct
    19441 ctgctgaatg agaacgagct agcgatcgca gatatatcgc gtaaccataa gaatgagcat
    19501 gtctacatat tgattgacat agacagtact gattttgaac agctcaatca agtgaaacaa
    19561 cagctagaaa aaatttctgg agtacgaaag gtccgcttat taaagaaacc agaaaggcca
    19621 taaagaaaga gaatatttgg atttcattaa ttcttctttc cgttctttac tttcgaattt
    19681 cgtacaacta aaataagaga agcaacattc ttttattgat agaaatgaag gagcaagcat
    19741 ttgaaaagat atataagcag actttcgacc aaacagaaaa agattttaaa aggaacagca
    19801 gcaatcatca gtatcttttt tcttgttgtt tttagtaatt tatttttgca gtggtgtcaa
    19861 aatgatctat ccgtagacct tgcgttgaaa tttgcttttt cttggcatac ggaaaaattc
    19921 ttcttagcct gtctcgtctt gctgattatt cttatttttc tgattgcact agcaggatct
    19981 gtccctttag gttcccttac gtatgtcgta gccatcggtg tattaggttt tgcaaattat
    20041 atgaaaatga gttatcggca agaaccgatt tatccagacg atttgaagat gatcactgaa
    20101 atcggattgc tgaaagatat gaccggcact atgctgttta cagtgatctt agctgccgca
    20161 ggaaccgtga taggattgtt ttgctggtac atgtttcgaa gcctgaaaaa aggaaggcga
    20221 ttccaattga tccgattaac gacactgctt gtggcaattg gactgctagg gtatatcagt
    20281 aatttcaaca atcccgataa tttgttgcga aaagcatata ataaaacggc actatggata
    20341 ccttacagcc agaaaatgaa ctactataac actggtttta tcggtgggtt tttgtacaat
    20401 ctgaaggtag aaccaatgga tgaacctgaa ggatattcga aagaaaagat caaagaaatc
    20461 actgaaaaat accagaagct agccgatgaa aaaaataaaa ctgtagagga agagtcgccg
    20521 aacatcgtgt tcgtgatgag tgaaagtttc tcagatccta gtcgattaaa cggcgtagaa
    20581 gtatctggtg agccattggc agattattat gaagtagctg atcaaacata tagtggcaat
    20641 atgctttctc aaaactatgg cggtggaaca gcaaatatcg aatttgaagc attgactggt
    20701 ttttctatgg cgttatttaa tgcgcaactc accacccctt acacgatgct tgtaccaaaa
    20761 atggatcagc ttccttctat cgtttcagcg ttaaacgcac aatcatacca gacgacagcg
    20821 atccatccat acaatacttc gatgtataaa agagaggatg tttatcagac attaggattt
    20881 gaccagttca tcagcgagcg tacgatgacc tatacggata cgatagagaa taatccatat
    20941 atttctgatg agtcagctta caaagaagta atgacacttc tcaaggaaga aaaaacacct
    21001 cagttcattc atttagtcac gatgcagaca catatgccct acgatggaaa gtatgataat
    21061 ctagactatc ctgcaaaaac ttcagatgga agtgggacac ttgacttaga gaattatctg
    21121 caagatattt cctatagcag cacggcactg aagcagttca cggaagaatt gaagaatctt
    21181 tctagaagga ctttagttgt tttttggggg gatcatcttc cagggatcta ttcagatacg
    21241 atccaagcaa aaaatgacaa acaaacattg catgaaacac aatttcttat gttcgacagt
    21301 aaaggagaat tagaaaagca aacgactcac gatgcaatca ccagtccgtt ttattttgca
    21361 gcgaatctaa tggaacagac gaatcagaca acaaatggct tttatcagct gcttttatca
    21421 ttagaacagg aacttccggc gtttgaacga gaactgtact atcaaaacgg acagtggtat
    21481 aaagaagcgc aattcaatcg ctcgcaacaa gagatttacg acgaatatca attgatccaa
    21541 tatgacattg tcgcagggaa acaatatagt ttagcagatg gtttctttga gcacgaataa
    21601 gaatggcgag ctgtatagaa accattaaat tttttcaagt acttgtcatt tgaggataaa
    21661 aacaagtaga ataaataata acaacaatgg agaaagtagg aaaacgaaca tgttaccgaa
    21721 tattggctat tatgtgcagt ccatcgatca aatcgtcaag gaaacagaag caatcggaga
    21781 aggaatgcat ccgaaatatg aagtgatcag aaacgcaatt gatgaaggaa aaacaagtga
    21841 actggacaaa tcgactcttg aagagatcat cctgttattt gaagaaggaa caaaaaaata
    21901 tcgggcaatg cttatgaaag tcagtacttt gcgtccacca gcaaaagcaa tgggcattca
    21961 taaaaatttt gaaaaagctt atctttcata tgtagcaggc tgtgaagaaa tgatccaaag
    22021 tatcgatctt gaatcagggg tagatgaaga tctattcaat acatctgaag caaaacaaga
    22081 ccaagcgaca gaccaattga cagctgccat ccaaaaaatg actaatttgt tgttgaaaaa
    22141 ataaaagaaa tctttgataa caataaaaac tagagttatc taatataaat gtaatattac
    22201 ctttatgata gagtgatcaa aatcctttat gataatgaca tacctataac caacaacaac
    22261 cttgtactta ctcaaaatcg agtaaatact ccttttccgt cgagtctaac catctcggcg
    22321 gttttttatt tgtagaaaag aacatcagga tagacggaaa agtcttgtcc tgatgttctt
    22381 ttcagatagt atggaaagct gattagtttt ctacattctg tacatattgt tcaaagtagt
    22441 tctgtaaatc ggctttgagt ttctgatatt cctcttcgct atactcttta tcttcgattt
    22501 tcccatcaaa gaaaggaata tttagttctt cccgtagtct gtctaatacg tcttgctgat
    22561 tcatttcatt ctccttttct tactaacgat caaaataaac acagacacat tctggtacat
    22621 agacatcttt tttcagtaat tccagtaatc ctgtttcagg atgacggcga tataatgtca
    22681 gattatcggt gttctgattg gcacacacga caaaattatt gcttggatct agtgcgaaat
    22741 cgcggggaaa atccccttct gttgagatga tttgcttgtt ttgaatgtgg gaaccatctt
    22801 caaaaactgc gaatacagca atcgagttat gtcctcggtt agaagcataa aggaacttgc
    22861 catcatttga aatgcggata gctgcccctc cattttcacc gtcgaattct tctggcaagg
    22921 tcgatatttt ctgtaattct tcaaaagcac ctgtttcttt attataagaa agcacgcgga
    22981 ctgtgctgtc taattcacca aataaataag cgaattgtcc atttggatga aagacaagat
    23041 ggcgagggcc tgttcctggc tctgcagtga aggtagttac aagattcaat ttgccgtttt
    23101 cggacacatc ataagtatac acacgatctg taccaagatc acatacgact aatcgttggt
    23161 caggagtcag atccgtatag tggacatgcg catgatcttg gtttttatga ggacctgtcg
    23221 cttcttgatg gaatattgaa tctgctgctt tcagttcgcc atctcctaat atatggtaga
    23281 catttacttc acctttatga tagttcgccc cgtatagtaa ttgacgtgct tcatcaaccg
    23341 ctacataaca caaaggagcg ccagactctg ttaccgcatt caaaaaatga aaatccttat
    23401 cgtacgcacc ggcaccacct aatccatcca cactagtaac tgtgtaaaga ttgccagctt
    23461 tactttttgc tacataggta gggctatttt cttcaacagc taatgaaagg ttggctaatt
    23521 cttctttttc tgtatcaagg tcgatcgtat agattccttt actttcgcga cgcgtatagg
    23581 ttcctaaaat tatcttttcg atcatatgag ctgattcctc ctaattgtaa tgaaatagtt
    23641 ttcgttttta ttctatcaca taattattcc acacggctaa agaatgagaa tgtgttatac
    23701 tgtttttgaa agcaattaaa ggagagagtg caatgattca ggcaaagatt acagaaattg
    23761 ggacagaagc aataaacaag gaagaaccca tgctgatttt gttcgataaa acagcaacag
    23821 ctgccttacg aaaatattcg gtgattcaag aagtatcaaa agacgaacct ttttctttga
    23881 aagcaggcgg aacaattgct tttgatgatc aggtatatac gatcgattat gtcggtccga
    23941 tggccaatgg gaatttaaag acagtagctc acgtatcatt gatttttgat gcttgtccag
    24001 aagaaggaca aattgcaaat ggaatctatc ttcatccgca tagactgcct gctattggtt
    24061 tgggaagcca aattttttat aactaacagg aggaattatg gaaggaaaaa ataagaagaa
    24121 cagaccggta tcgtggtttt ggcgctggtt tttaaataat caagtagtca cagccttact
    24181 tgttgtattg ttaatattgc tgatcgtctt tttatttaca aaagtttcat atttgtttgc
    24241 accaatctgg caatttttag caatcgtagg tctgcctatc attttagcag gaatcttgta
    24301 ttacttgatg aatccggtag ttgattactt tgaaaagcga aaagtaccaa ggctttacag
    24361 tatcattggg ctgtttgttt tggttgttgc actaatcgca tgggggagcg tagtgatcat
    24421 ccctaagatt caggaacaaa cagtgagctt catcagtaac ttccctcaat atgtagatac
    24481 gattgataat aaattaaccg agattttgcg ggacccgctg ttcaaccaat ttcgcgaaca
    24541 gttggaaaca gcaggtgata agttcatgaa ttcggcaggc gatatgatcc aagatatatc
    24601 taagagtaca gttcagagtc ttggaagttt tgtcggcgcg gtagcgacta ttttagtggc
    24661 tctgctgacg atgccgttta tcttatttta tctattgaaa gacggtaaac aattagcacc
    24721 ttattttgtc aaatttttac cgacacgcat gcaaaaaccg acgttgaatg tgctgagtga
    24781 aatgaatagt caagtttctt cttatataag aggacaacta actgtggctt ttgctgtggc
    24841 aatcatgttt atgattggtt ttgcggtcat tggtttagag tatgctgtca cgttggggat
    24901 tattgcgggt tttttaaacc tgatccctta cttaggatca tttttagcga tgattccggc
    24961 tatcttttta gccattgtag caggtccatt catgatcatc aaagtactga tcgtttttgc
    25021 agtagagcag acaatagaag gacgcttgat ctctccatta gtactaggga atcagttagc
    25081 aatccatcca gtaacgattt tattagttct gctgacttct gggaaattgt tcggaatcat
    25141 cggtgtgatc ttggggattc ctgtttatgc agcagctaaa gtagtgatta ctcatgtttt
    25201 tgaatggtac acaacaatct cttctcttta tgaagaaaat ccgttgaaaa agcagaaaga
    25261 ataacaaaaa gagacagatg ggaaaatatc caaggcacaa atgctgcttt gaagattttt
    25321 tcctctgtct cttttgtatg catttatctc aatttttaaa atgatttacc taaagcagca
    25381 gctttatcta atgcattttg catcaactca tctgcacggt cagggtcata atcgattcct
    25441 tcgatgaata gctgatctac ttgatcgatg cctacaaagt ttaaaatacc ttttacatat
    25501 tgagaggcaa agtcttgtcc gttgtataca ccgccattag attggatgtg cagtgctttt
    25561 ttacctgtca ctaatccttc tggcccgttt tctgtatagc ggaacgtttt tccagctaca
    25621 ttgacggtat cgaaccaagc ttttaatttt gttgggatat ttagattcca taaggcgttt
    25681 gcgataacga ttttatcagc agcaaggaac tgatcagtca attcattgaa acgagcaatt
    25741 ttttgttgtt gactttctga taaggaagtg aattctgcac cagcacgtaa tgcttcccaa
    25801 cctgataata attcttcatc gatttccgga atatcatcgc gataaagttc caaaacagtc
    25861 acttcatcat ctggatgatt ttctttataa cttgttaaaa aagacgtcaa tgccttaact
    25921 gtgcgcgatt cttctgcagt taggggatga cctttgacta ctaaaacaga tgccatatgc
    25981 ttaccttctc cttttattgc tatgattgtt ataactatga ctaacaaaat cagtttactc
    26041 gaaaaagaaa aggctatcaa gtcatattga taaggttgaa gaataaataa ctaaaaaaag
    26101 acagggtata aaagaggctg tgacacgatt ttttgtcaca gcctcttttg aatctaacaa
    26161 gcaatattca agcaacactc gactgaagag cttccatccc accaatgata taaacagcac
    26221 gaaaaccatt agcagtaagt tgatcggcta atgcttctga tcgcctgcct gtatagctta
    26281 gaatataata aagtttcttt ggatctagat tttttaattg tcttggcaag atagcagcgg
    26341 ggatagaaag cgagtggctt aagtggtttt ttgtgtaatc taaggtattt cttgtatcta
    26401 aaacgatgct gtcagcatcg agaggcaatt tattgaagtc ttggacacta attgattggg
    26461 ccatttatct catcctttgt ttagtatgtg gctattttaa tgggcaactc tcattaattc
    26521 aaatggaagg attcctctta tcactttttt aatgtatcgg atgataatga aaaaaactat
    26581 gcaaatgtct tgcaaaacag gtaggaggtg ttataatgac atagcgtcat atgacaatgt
    26641 gtcatcttga aagaaaggag aatccatatg ccgacacaga catttttcca tttgcctaaa
    26701 gaaaaacaaa aaagactaat cgaggctgcg agaatcgagt tttctagagt acccttaaaa
    26761 gaagcttcga ttgctaatat cgtcaaatta gcggatatcc ctagaggcag tttctatcag
    26821 tattttgaag ataaagagga tctttatttt tattactttg agacaatgag acgtgacagc
    26881 tctaaagata tgttacaact aatgaaagaa tccgatggtg atctattcaa aggattcgaa
    26941 tcctattttt ctaaaatgat ttatgagatt ttggaaggcg agaatgcttc tttttatcgt
    27001 aacttgttta tgaacatgga ttaccgtgcc tcaagaaaag tcgctcctca tacgagtcgc
    27061 caaaaaacgc ctcacgatcc acataaagaa cacaaacaag atgcaaaaga gttgcttgaa
    27121 ttgatcgatc ggacgaagct gaaagtaaaa aatgatcatg aattggaatt actgatccaa
    27181 ttagtgatga atactgtatt tacaacaatt gcgcattcgt atcgcttatt ttcaacatct
    27241 gaagattact cagtagagca agcaatcagt gaattcaatt tgaaactttc ttggctgaaa
    27301 aacggagtat ataaatagaa agaagggaac tagatgctaa aaatcgtaaa acggatctcc
    27361 ttgacttcag caattgcagc tgcagttttc atggtgatcc aagtactggc agatttatat
    27421 ttgccgacac ttacctccaa tattattgat aaaggggtag cgcaaggaga tgtcgattat
    27481 atttggcata caggttttgt gatgatcggc ttttctttga tcagtatctt agctgctatc
    27541 gcaaatacat tttttgctac tcgtgaatca cagaaactgg gtaagcaatt gcgtacggat
    27601 gtctataaaa aatcagagag tctgacaaaa gatgcgtttg ataaatatgg aacggcctct
    27661 ttgatcacaa gaacgaccaa tgatgtgact caaatccaaa tggttacaca aatgttttta
    27721 cgtatgatga tcaatgcacc aatcacgttg attggcgcga gcatacttgc ttatcaaaaa
    27781 gatcatcagt tgacaaaaat cttcttagtt gtcatcccag taatgattat tttgatcggc
    27841 ggaatcatgt actttgctgt tccattattc aaaagtatgc aaaagaaaac tgaccgttta
    27901 aacctggtat ttcgagaagg attgacaggg gtcagagtga ttcgagcatt cgataagaca
    27961 cgctttgaag aaaatcgttt tgatttagcg aataaagatt atacaaatac ggcaatcaaa
    28021 gttaatacga tcgtcgcatt gatgatgcca atgatgacat tgatcatgag cggtacaaac
    28081 gtagcaatca cttggtttgg tggacactac atagcagata tgactttgga agtcggtaat
    28141 ctgattgcat ttatgactta tgcgatgcag atcttgatca gctttatgat gctttccgct
    28201 atttttatca tggtgcctcg tgcacaagct tcagctgacc gtattaatga agtgttggat
    28261 gaaaaaatcg gtatccatga ccctgaaaat cctaaaacag tttcttttgc tggaaaaaac
    28321 gcaacattgg ccttcaatca tgtcaattat cgatatcatg gagcagaaaa acttgcattg
    28381 gaagatattg atttccaagc caagtcgggc gagatcgtcg caatcatagg ggggactggt
    28441 tcaggtaaaa cgaccttagt caatttgatc cctcgattat atgacatcga atctggttcg
    28501 atccaaataa atggaacgga cattagtgac atgactcaat ataatttgcg agaattaatg
    28561 ggctttgttc ctcaaaaagc agttctgttc tctggaacga tccgagacaa tatgcagtat
    28621 ggtaagccgg atgcaacaga tgaaatgatt tggaaagcat tagaaatcgc acaagctaaa
    28681 gattttgttt cagagatgga agatggctta gatagccatg tggaacaagg aggaggaaac
    28741 ttctctggtg gacaaagaca gcgtctggca attgcacggg cattagtcaa aacagcagat
    28801 atctatgtgt ttgatgattc cttctcagcg ttggatttca aaacggatgc caatcttcgc
    28861 caagcattga aaacgaatat gaaagaaagt atcaccgttt tagttgctca acgagtaagt
    28921 acagtaatgg atgcagatat gatccttgtg ctagatgaag gaaaactggt tggcaaagga
    28981 acacatgaag aactattagc aacaaacgaa acttatcaag aaatcgttca ttcacaacta
    29041 agagaggagg acttagcatg agtaaaacaa acacacgtcc ctctcatgga ccagcaggcg
    29101 gtccaggaca tggtggacct ggcagaaatc tcggtgtaaa aggtgaaaag cctaaaaatt
    29161 tctggggaac ggtacgccga ttgtttggct atatgtctaa acgtatggtt gcgatcatcg
    29221 ctgtatttgt actagcaata gcagccgtga tcttccaaat ccaaacacca aaggtcttgg
    29281 ggaaagcaac cacagaaata ttcaaaggtg taatggctgg cgctcaacag atgcaacaag
    29341 gccaacaagt aacaaaattt ccgatagact ttgataaaat cgcgcagatc attgcgacag
    29401 taatcgtgat gtatttgatt tctgctgtat tcaacttttt acagcaattt attatgacac
    29461 gtgtttccca acgaacagtt tatgaattac gtcatgattt ggaagccaaa atgaatcgtg
    29521 taccgatttc ctattatgat acacatacaa atggggatat catgtctcga gcaatcaatg
    29581 atatggataa catcgcaagc acgctgcagc aaaatttgac tcaatttgta actagtgcag
    29641 tcacattgat tggggtcatc gtgatgatgc tttcaatcag ctggcaattg acgttagtcg
    29701 cgttattgat gatcccttta agcttgatca tcgtgatgat cgtagcgccg aaatcacaag
    29761 tgtttttcgc agatcagcaa aaaagtttag gtttattgaa taaccaagta gaagaaacat
    29821 atggtggtca taccattgtt aaaacattca atcatactga aaaagaccag gaagtatttg
    29881 aaaaagaaaa tcagaatctg tatgccgctg gttggaaagc tcaatttatt tcggcaatca
    29941 tcatgccgtt gatgaatttc gttaaaaatc taggctatgt gttcgtcgct gtactaggcg
    30001 gagtaaaagt tgcaaatggg aacatgacat taggggatgt tcaagcgttc ttgcaatata
    30061 caacacagtt ctctcaacca atcactcaac ttgcaagttt gatgaacact atccaatcaa
    30121 cagttgcttc agccgaacgg gtatttgaag tgttggatga agaagagatg tccaatgaaa
    30181 aatctggcct tccagaagaa aaagatacac catacaaagt tcgttttgaa aatgttcaat
    30241 tcggctacac accagataac ttgctaatga ctgattttaa cttggatgtc aaacctggtg
    30301 agatggtcgc tattgtaggt ccgactggtg cagggaagac gacactgatc aaccttcttg
    30361 aacggttcta cgatgtttct ggaggtagta ttcgttacga tggtgtagat acccgtgatc
    30421 tttcacgtga tgaattgcgc gcacagtttt caatggtact gcaagatact tggctgttta
    30481 caggaagtat ctatgacaat atcaaatatg ggaacgacga tgcaacagac gaacaaatca
    30541 ttgaggctgc taaagctgct cacgtagacg attttgttcg taaattgcca gaaggatata
    30601 acactgtatt aaacgaggat gcaagcaata tttcgcaagg gcagcgtcag ctaatcacaa
    30661 ttgcgcgggc attcttagca aatccagatg tcctgatcct tgatgaagca acatctagtg
    30721 tagatacacg aacagaaata ctgattcaaa aagcgatgaa ccgcctattg gaaaaccgga
    30781 caagtttcgt tgttgctcac cgcttgtcta caatacgcga tgcagacaac attattgtga
    30841 tgaatcatgg ttcgatcgta gaaacaggaa accacgatac attgatggaa aaagacggat
    30901 tctatgcaga tctttataac agtcagtttt ctgaagaaga agcttcatag aatagtacaa
    30961 atttattatt ttttttaggg gctgtgacaa aagtcgtgtc acagtccctt tttccgaata
    31021 aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgagaagc
    31081 tatttttccg ttttgtatct ctaatactca ggtcaaaccg ctgattttac aacctcttcc
    31141 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcaa caaacctgag
    31201 aagctgtttt ttgaaattgc tccttgtgac tcggagttaa acggcaggct cacaacctct
    31261 tggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
    31321 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
    31381 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    31441 taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca
    31501 acctcttttt ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt
    31561 tctcacattt tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta
    31621 aaataaagct tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc
    31681 acctatttaa aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac
    31741 aagaacgttg ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca
    31801 gaagaagctg gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg
    31861 ggggtgatca aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca
    31921 gatatccttt gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat
    31981 ggacgtatgg ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa
    32041 gagaataaga acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga
    32101 aaatctgaag aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta
    32161 aatgttacta acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta
    32221 cataatgaag aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca
    32281 gaagctagcg gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg
    32341 aaattcatga tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca
    32401 gttgatttct tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc
    32461 gaatggtggt tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct
    32521 taactttttc tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat
    32581 cgcttattct agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg
    32641 cgcagttttt ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg
    32701 taagaatctg atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt
    32761 gaaaaagatg gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca
    32821 agtagaaaca tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga
    32881 agatctgttg tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt
    32941 acgtctatta gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga
    33001 atttgaaaat cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac
    33061 atggatacca aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct
    33121 agaaccaggt gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct
    33181 tgggttgatg ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt
    33241 attggaacca ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt
    33301 tcccacagaa caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc
    33361 aagagttttg gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat
    33421 tacttttgtc caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga
    33481 agagacgatt gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt
    33541 aggatgggac ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta
    33601 tacaggtacg tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa
    33661 tcgcgtacat cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga
    33721 aaactttttg aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg
    33781 agaggagttt gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt
    33841 tggggaggaa gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga
    33901 gaagattggg cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc
    33961 aaagggaaaa gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca
    34021 gaaccagtgt ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa
    34081 gtacatccag atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc
    34141 tggtatatca tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca
    34201 agagaagaac tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta
    34261 ccagttaaaa aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa
    34321 ggaatcatga ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac
    34381 gacagaaaag atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta
    34441 acaactgtcc ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca
    34501 tcgatcgtca cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga
    34561 atcacatctt tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga
    34621 gaccttgtcg ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca
    34681 aatggcgttt ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc
    34741 aaatagttcc cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat
    34801 aaaatccgtc catcaataaa tagaagtctg gtgaaaaaga tggaatacaa atggaaagca
    34861 aacaacagca gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa
    34921 atgatcatgc caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa
    34981 ggtgtgtctg gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg
    35041 cttttaccca tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca
    35101 gtcgccggtg caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta
    35161 ggagcgatga ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc
    35221 catgtgaaat tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact
    35281 ggtagcgtta tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac
    35341 ttgttgctcg cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt
    35401 ctgatcttag aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg
    35461 acacctttag gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag
    35521 gcaaatcctg gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag
    35581 gaaaaagtaa atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc
    35641 tattttccat ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca
    35701 agcggcacgt tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg
    35761 tcactactcg tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg
    35821 atagcaatca gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat
    35881 aaagaaaaag agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt
    35941 cattccgtca tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg
    36001 ctgaaaaaga aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat
    36061 gaattgaata accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca
    36121 agagaaaaag tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc
    36181 ttgctgtcac gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata
    36241 gcagatatca cagaacaaaa aacgtcgcct gctaatattg tatttttgta taaaaacaac
    36301 agacgcggct cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa
    36361 aaacagtttg ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt
    36421 tatatcgcac aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta
    36481 gttctagttg aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata
    36541 taagatgtat ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc
    36601 acctgtatct atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg
    36661 cgaattagat aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc
    36721 tagaggctca tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac
    36781 agatgggaaa agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt
    36841 gttactgacg acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa
    36901 taccactttt tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga
    36961 aatcattcgg aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac
    37021 agcgaatgta ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga
    37081 tgcatcactg aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa
    37141 agtaatcggg gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca
    37201 tttggttttg atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa
    37261 tgagacatat aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc
    37321 acggatcgct tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg
    37381 attattaaat gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac
    37441 acagcttgct tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca
    37501 tttttatgaa gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc
    37561 aagagccgtt ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca
    37621 atacgaggaa gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa
    37681 attatccgaa gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag
    37741 tccgaaagaa agcaatatca gtattgcagg attttcgcca agtggattag cttctactag
    37801 catgttggaa ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg
    37861 gattgctgat ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc
    37921 tattttacca ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga
    37981 agtcaagcag ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac
    38041 gtataacagc aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat
    38101 ggatcaaatc aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac
    38161 gatttccgaa acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga
    38221 agaggtggtt catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca
    38281 tacaaatatg gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat
    38341 tttcaatttg aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc
    38401 ccgtatgtta gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg
    38461 aaagatcagt ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa
    38521 taaagaaatc gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag
    38581 gtgaaaagat gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt
    38641 ggaaagaagc gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg
    38701 catatatcca atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg
    38761 tagccttgcc acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag
    38821 tccaagtgcc tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct
    38881 tatttgtagt ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt
    38941 tctgttcaga catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc
    39001 aaatgatttt tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc
    39061 atcaagtaat aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct
    39121 tattactgcg gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat
    39181 aggggacggt gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt
    39241 ttttatatag atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt
    39301 caagatacca ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc
    39361 tatgatcaca ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat
    39421 ccatccaatc gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa
    39481 aagattgact agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa
    39541 gagcacccac aacaacagga acagaaagct ctggaaaaga tactcataca tcggcaggat
    39601 tcgtatctcc tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt
    39661 gattattact agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc
    39721 ataataaatg gtaaaacgga tgctatgttt ctttaccata ctatccagta atagattgac
    39781 tagagagatt acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt
    39841 tatacataaa atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc
    39901 taaagagaga tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat
    39961 ataacggagc aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc
    40021 agaaatcagt cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg
    40081 gacattccct gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa
    40141 actttccaca aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat
    40201 ctcttcatag ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa
    40261 aagtgggaat gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt
    40321 tagtaagaga ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga
    40381 taaatagcta aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga
    40441 aaacatgctt tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc
    40501 gtcttcccct ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg
    40561 tctaatccgt tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca
    40621 gcaagcaaga catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac
    40681 tcagtcatct tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat
    40741 ttgatcaaag tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct
    40801 agaaaaaaga aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac
    40861 aattgatcat aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca
    40921 aatccgttcg gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt
    40981 cgaatcacac gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat
    41041 aggcctccaa tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta
    41101 acaaaagtta ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga
    41161 agttttctga taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt
    41221 ttcgtgccaa agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa
    41281 ggagagaggg agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc
    41341 gagggtacaa aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact
    41401 gattgcctgg ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc
    41461 tttcgctacc atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac
    41521 tggcggaaaa gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg
    41581 tgtgattgtc ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg
    41641 tggatatgcc atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga
    41701 aatgctagtc aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt
    41761 ttctgcgatt ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat
    41821 cattttatct gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt
    41881 atttttgaat aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc
    41941 agaggctgga aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt
    42001 attacttgct tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat
    42061 gatcattcac tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc
    42121 gttattattc ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat
    42181 ggatgctgga ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac
    42241 accgttcaat tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt
    42301 tggtatttct gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa
    42361 atctgttcaa gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca
    42421 aggagctaca ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc
    42481 aagtgcgatg ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac
    42541 ggtgaccaat caagcaatca gccaactgac agatgactcg aatacattga tcgttactca
    42601 ggtcgaacta caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga
    42661 aaactttttg aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa
    42721 aacatcagtt tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa
    42781 acaaatcaaa gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat
    42841 gaccgtgctc aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa
    42901 tatccaagat ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga
    42961 aaaagtacag atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc
    43021 gcaaaaatac gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc
    43081 atgcaaagtt tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa
    43141 gcgatccgct attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc
    43201 gatgccatgg tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt
    43261 cctcatggaa cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa
    43321 gtaccagatg gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc
    43381 ggtattgcag gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc
    43441 agtgatatgg acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa
    43501 aatctagcaa ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat
    43561 atcggtcgag gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta
    43621 gatgtgaatc acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta
    43681 gcagatgaat caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca
    43741 aatccagaaa aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc
    43801 ccgaatatct taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag
    43861 gaaggaagca caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca
    43921 tttctagaaa aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc
    43981 ggctttccaa atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta
    44041 ttcgtccaag tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa
    44101 aaaatccaat taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa
    44161 ctatttagtg tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac
    44221 cagaccattg atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg
    44281 caggaaacag gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg
    44341 tatattgaaa aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt
    44401 gtcgctcgga caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga
    44461 gaactacaag aacataactt gacttgccca catttgacgg caacaatggg gattattttt
    44521 aattactatg atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa
    44581 aacttggaac aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat
    44641 atcaagcaaa acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg
    44701 ggaatgtgac gaaatttttg tcacattccc ctttttcgaa taactaggct gtttgtcctt
    44761 caagataggg cagatccaat tctttaccgt aacgttgttc taaagctagg cggatcaaac
    44821 gagctgccag gttctcattt cttgcaagga taggtccatg gaaataagaa ccgaagacat
    44881 ttcggtaaat gacaccttca gattggtctt caccattatt tccttgacct tgtaccactt
    44941 tccccaaagg tcgttcacct tcacctaaga aagtccgacc attgtggttt tcaaatccat
    45001 aataagtttc attgaattct tcgttatgga taacgatatc accgataaac cggttatttt
    45061 cctgacttaa cgtgtagtga tccagtgcac tgattccttg gatcttttct ccattagctc
    45121 ccatgtagta gtgacctagc aattggtagc caccacaaat ggctaacatg acgccatcat
    45181 tttcgatata gttgatcaaa gaatctttct ttttctggat gtcttttgaa atgattaatt
    45241 gctcaaagtc ttgaccaccg ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt
    45301 catgtatact aacgatctcc gtatggcaag aaacccccat tttttctgct atatatttca
    45361 acatcaaaag gtttccgtta tcaccatatg tgttcatcaa atttccataa aggtgggcga
    45421 tattgagttc ataattagcc acggttcatt cctcctttga taaatccttg agcagctagt
    45481 tctttacgaa gctgcaatac agcagtgtaa gtagctaaaa tatagacatg ctcagttggc
    45541 aattctttga ttttttcgat tacttctggt aattcttttg tttctgtcaa tttattttct
    45601 ggaatcccgg caacttttag acgtaaagtc atatctgtat gacggtctcc tccagaaata
    45661 acagctggga tatccatatc ggcaaagttt tcaaaattac catcccaaat ccagctgaca
    45721 tcgattccgt ctgcgtaatt ggcatttaat aaagccacta aagaaaatgg ataaggagct
    45781 agaccaatca tatcgattac ttgattgata cctacaggat ttttgacaag tactaatgta
    45841 cattttttcc cattgatatt gatgacttct tgtcggccga atactttctc gtcatatcct
    45901 aaaccttgtt tgattttatc aggatcgaca ttgaagtact ctgccactgc agtagctgct
    45961 aaagcattgt agacattata cataccgcca acttcgattc cgtattcatg accatcgatc
    46021 acgaaatctg ctgacacatt gtccatccgt accatatcag ttaatcgata atccaattca
    46081 ggacgtttga agtcacaatt tggacagtaa tatttcccta agtttgcata agtgatcatt
    46141 ttataatgca agatgtgatg acaatttgga cataagacac cgtctgtatt gtaatgagcc
    46201 atttgttcac gatcttcttc atggtcaaag ccataatatt ttctaggatt gactgtttcg
    46261 atagaattga agattggtga atcaccgtta ctgataatcg ttgcatttgg tgactttgcg
    46321 gcaccgtcta caatcatttt ataggtcgta tagatttctc cataccgatc catttgatca
    46381 cggaaaatat ttgtaaataa gaacagttct ggtttcatat attctgtgac tttgctcaaa
    46441 cttgcttcat ctatttctag gacagcaaac tttttctgtc ctttttttgg ttttgcctgc
    46501 aagaaagtcg atacaattcc ctgtaccata ttcgcaccag tagggttggt tagaacttca
    46561 tcgaattgct ggcgcaaaat attgactgtt aaagcggtag tcaatgtttt accgtttgtt
    46621 ccggtgacaa cgacgacttg gtagtctttt gccaaactat ctagtatatg tggatcgact
    46681 ttcagcgcta gtttacctgg taaacttgat ccgcctttaa aaaaagtttt taatgcccat
    46741 tgcgacgttt ttccaactgc aatcgcaaaa tgactgcgta ttcccattat gtaacctcca
    46801 aaaacgattt gctgacgaat aaattcaact aatcatacca tatgttgaaa aaaaggttaa
    46861 attgcttttc ttattcttaa gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat
    46921 cgcatgattt aacaaaaaaa cagcttgaaa caaaatgatt gtttcaagct gctgtgattt
    46981 tattattccc tcttacctat attcccattt taaccgataa taattttctc gcttggatat
    47041 tcatagccta gatctcgtgt ttcttttggc acgaataaca tcaatgtata tagcattccg
    47101 atccgcccaa taaacatcag tgtagcaatc gtgattttgc ccacaacaga caagtctccg
    47161 gtaatcccta aagataaacc agttgttcca aacgcagaag ttacttcgat gatgatcgag
    47221 atcagcgttt gttcttctgt agcagataaa aaaacgatac aaaagaaaca cataccaaga
    47281 gaaagcataa agacaacgac tgattttcgc acatcatctt gatcgattct ccgtccaaaa
    47341 atattgatgt tatcttcact tttgaggaag gaatatagat acaaaccgat gattgctaca
    47401 gtagttgttc gaatcccgcc gcctacagaa ctaggactgc atccgataaa catcagtaaa
    47461 gaaaaaatga tcagtgttgt gatttgaaaa tcacccaaat cgtgaatctg cagaccagca
    47521 ttacgagtcg taatcgaata aaacatcgaa ttgatccatt tgacactcat attggaatct
    47581 tggaataaat ggtccttttc caatagataa atcaagacag ttcccgaaac aaacaagata
    47641 acaaaagcta acacagctaa tttggtaaac aaggaaaaac gaaacggaag cttcgcattg
    47701 gaacgtttgt ataatagcca ttcccggcac tccatcaata caggaaaacc aattcctccg
    47761 acaaaaatca aaaacatgat caggatcaag aaaaaatagt catgtgcaaa gggcttgatg
    47821 gaatccccag tgacgtcaaa cccagagttc gtgacagccg atatcgcctg ataaaaaccg
    47881 taaaaaacgg catcacgcca acgatcaaaa tagcctctgt aataaaaata aatagaaaag
    47941 aatgtcccaa aaagtaattg aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg
    48001 acgataccag acaaacgcgg ttgattcata tccgtcatga tcagctgtcg ctgtttcagt
    48061 gtgatccgtc gtttagaaaa aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca
    48121 ccaacttgaa ataatatttc cagcagtata ataccgttat cattgaacac agaattgata
    48181 tcaaaagtcg atagtccagt cacactcact gtactgattg ccatgaacag catgtccaca
    48241 aaagaaacat ggctatctgg ttcgcgaaaa atcggcatat aaaatagaac gagtgataag
    48301 accgtcatca atatataata aaaaacgatg atttggatcg atgagaaatg attggcagca
    48361 aacttctgtc ctcgccgctg cagccttcga aagaataaat caacatgcac agtactatcc
    48421 tcctgacaat agtcgtttct accagtatac aaggggaatt aggaatactc aaagaaatta
    48481 tcaaacagat aacaatacag ctttcgatta aaatagaaat ggcttataat aaggaaaatc
    48541 ctagaaaaaa gaaggaggag gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa
    48601 atgaaaaatc aaaaaataaa ttatgataca gttctgaaaa aaatgatcat gcaatgggag
    48661 aaagaagaag taaagcctaa gatattaata catacatgct gtgctccttg cagtacgcac
    48721 acattggaat tcatgtctca atatgcagag atcacgttat ttttttcaaa ttcgaatatt
    48781 catcctaaaa gtgagtattt gcgaagatta catgaacaaa aacgatttgt agaccaattt
    48841 aatgagaaaa ctgaccatca tgttgcgcta atcgttgatg aataccgtcc aaatgaattc
    48901 accaaaatgg ttttaacaaa ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt
    48961 gcatgcttca atatgcgatt agatcttgtt gcgcaaaaag cgcaagaact aggatacgat
    49021 tactttggaa gtgccctgac catctcgcca aagaaaaatg cagctttgat caatcagatc
    49081 ggaatggata tccaaaagat ttacgggaca caatacttgc caagtgattt caagaaaaac
    49141 aaaggctatg aacgctcttt gcaaatgtgc aaagagtatg atatttatcg ccaatgttat
    49201 tgtggttgcg tgttcgctgc caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa
    49261 gcaaaagctt acttaaaaac atcaaccgta gtttttgagg aaaattaaag aaatctttcg
    49321 gaaaaacgta agaaaaaaat aaagaattta tggtagaaat aaggtagact gtataatttg
    49381 tccgtagtaa agtaaggaaa caagaataaa tttcaatgaa ggaagagata agatgcgaaa
    49441 agtagcacct attttgaagc caatcgctgt attaacaatc tcgacgattg tcgcgactac
    49501 tacaggtatt gtgaatagtt ccagctaccg tgcaccaagt tcaagttcta gtactgtaga
    49561 agaaacaaca ccaactactt cagtctcgac atctgaacca acaacaacgg aaagtttgaa
    49621 tgaaaccact acgacaagta catgggacga tacaacaact agttcgtcgt atgatgagac
    49681 atatacaact gacagctcaa gcgaaacaag taatacggta ccagatggca ctccatcgga
    49741 ttcaaccaat ccaagtacaa gtccggatac aagtgctagc tccgaaacga taccaagcag
    49801 tacgactgta gatgataaca cgcaaaatga tgatgcagca cgtacagcac agcaaaacca
    49861 agattcagga aatacccaaa cgtataacca gcaagcaggt gattctcaat gacattttta
    49921 ctagacctta tcaacggggt gcataaattc cttggctatt tagacatcag tcctaaatat
    49981 cttaaccgtg gatatacgat tttaagtgtg attcccacgc tttatttatt gagaattgtt
    50041 tatggacttt ggcaaaacca aaactatctt caatttttct tatatggact tgcatttcta
    50101 gtgcttgttt attttactgt tttaaatgta ttttattact ttttagataa aaacacgaaa
    50161 gccgatgtca ctcagctttt tgtgaagtat ttacctgatg aagcgttcaa catccagaca
    50221 gaagaatcga tagataatgg aaattcgatc gataaagtga atacacaaga ggtcatggtt
    50281 tcttatgacg aagattatca attgaaatta gccgagaata tgcggtattt gattggaaat
    50341 ggtgagatca aaacaaatga actcggtcgc atggatggat ttctggtcga tagaaataca
    50401 ttgtatcctt attactatgt caagaaaaca ggagagaaag agtatgttct aaatattgga
    50461 cgaagctact cggacatgga aaaagtaggg accattcgac ttgactcaac agaagacaca
    50521 ttggaacctg tcggattgtt catcgtcggc ggtgattttg tcaaagacgg gttacgctat
    50581 catgagcctt atcgtctgaa attgattgca aaaaaggcac agtcgcagga agaaccagtg
    50641 gtttatagtc gtagccaaag aagaagacat tcataatgtg atgaaacgcc gtgtatcagt
    50701 ggttgcgata aaaacgttta ttcggaataa gggggatgtg acacgtcttt tgtcacgttc
    50761 cttttttata taacgagaaa gataagacga acacatcact tattctttcc tttcatttat
    50821 aaacattgta gaataaagaa agtacaacga agtattgtga aaaaacgtaa taaataacga
    50881 ggaaaataca agtaggaggt aagctgtgaa agaatttttg aaaaattggg ggattttgat
    50941 ccttgtatta gcggctattt tattagctag agtgtacgtg tttacaccag ttactgtaaa
    51001 cgggcattcg atggacccta cattaagtga tggacaacga ttgatttcat ctaagatctc
    51061 aaactatgaa cgaatggata ttattactac aaaggaaccg ggggatgaag aacggatgat
    51121 tgttaaacgg atcatcggta tgcctggaga tacagtcaag atggaaaatg accagttgac
    51181 cattaatggc aaaaagtatg atgaacctta tctggatgag ttcaaaaaag aattttcaga
    51241 agataagtta caaggagaat atgcttatag ctcaggattc caagcacaag cagaaagttc
    51301 atccaccttt acgagtgact ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg
    51361 cgataaccgc ttgatttcaa aagacagccg aatgtttggg ttagtcgaca aagatatgat
    51421 ccaaggaaaa gttgttttcc gttattggcc tctttcagaa attaaaatca tgtgatgaaa
    51481 aaataaaaag aagctgtgac atgttttttg tcacagcttc tttttccgaa aattccttct
    51541 tatttcttgg agttgctcac tttttttgca atctcatttt aagctttttg gctgatggat
    51601 aagaaaagcg aagcgttttt gttcataaat agcttttccc cacgatggaa ggtatgacta
    51661 gtagcagaga caaagcccgt tttttccaat cgattattca attcttcttg gctaaagcca
    51721 ttatggactt tcggatgact aatttgttca ttcttgtcaa agtctacgat gatcaattga
    51781 ccgccaggag ctaaaatttc ataaagtttt gtaagaatat tctccgtatc aggaatgtgt
    51841 aaaagtacaa gtgatagaag gatgagatta gcttggacag caggaaattc gacagaagca
    51901 tcagcgtgga tcgttcggac atttttcaga tctgctgctt ggatcttttc ttctgccatt
    51961 ttcagcatag tttctgaagc atcggcaatg atcagttctt tgaatctttc cgcaagcggt
    52021 aaactaacaa gcccagttcc gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc
    52081 ggtagataag tagttagttc ttcgcgaata atcttggcta atgcttgacg gtcaggggaa
    52141 tcataatggt tagcgatgga atcaaaaata ttttttgtca tcaaatagct ccttacgtaa
    52201 aactttgaaa ttttaactta ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat
    52261 tcaaaaaggg agaatctaga aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa
    52321 aaggaaagtg tgtccgagaa atattactag acttgcctga gtggtttggg cttcctgaat
    52381 caacggaaaa atatatcgag gaatcaagta aactaccttt atggtgtgaa aaaagaaaag
    52441 aagaatattt aggctttatc actctttcac agacaagtga agatactgcc gaaatctatt
    52501 ctattgtatg ggaataaaaa aagactatca tcaccaagga ttaggaacaa aactttttaa
    52561 agaagcagaa ggttacgcag caaaacatta caaatatttg caagtgaaga ctgtagacga
    52621 ggggcactac tctatatatg atcaaactat ttgtttctac gaatcattag gattttcaag
    52681 gttggaagtt tttccaaatt tatgggatga atggaaccca tgtttagttc ttgtcaaaaa
    52741 attggaacaa aagtgagcaa tagatttaat actaatctgt gcttgatgaa aattctcact
    52801 gtaaaaaatg ctgaaaatca agtataatac cataggaaca aactggaagg tgaaaaagag
    52861 catggcacaa ctatttttta agtatggcgc aatgaatagc ggcaagacga tcgagatcct
    52921 aaaagtcgca cataattatg aagaacaaaa taagccagtt gtcttaatga caagcggtat
    52981 tgatacaaga gatggtttag gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat
    53041 gccgatattt gaagaaacag acgtatttga cgtaattcag aaaatggaaa gcaaacccta
    53101 ttgtgtgctg atcgatgaag cgcaattctt aaaaaagaaa catgtcattg cttttacgag
    53161 aattgtggac gaattggata ttccggttat ggcatttggt ttgaaaaatg attttcgaaa
    53221 cgagttattt gaaggatcaa aatatttatt gctttatgca gacaaaatcg aagaaatgaa
    53281 aacaatctgt tggttctgcc acaaaaaagc aatcatgaat ttacattata ttgatggaaa
    53341 ccctgtttat gaaggcgatc aagtgcaaat tggcggaaat gaagcttatt accccgtttg
    53401 tcgaaaacat tactttcacc catcaatgat tacagaagga gaataagcat gtacgatcaa
    53461 ttacaagcaa ttgaagaccg ttatgaagag cttggagaac ttttaagtga cccagaagtg
    53521 atttctgata caaagcgttt tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca
    53581 gtcgaagttt atcgtcgtta taaagaagta gtacaaggaa tcaccgatac agaagaactg
    53641 cttggtgaaa aactggatga tgaaatggct gaactagcaa aagaagaatt atctgaatta
    53701 aaaaaagaaa aagaagtctt agaagaaaaa atcaagatct tgcttttacc aaaagatcca
    53761 aatgacgata aaaatatcat catggaaatc cgtggagcag caggcggaga cgaagcagca
    53821 ttattcgctg gcgatctttt caatatgtac caaaagtatg ctgaagcaca aggctggaaa
    53881 acagaagtga tggaagctaa cgttaccgga atcggtggat ataaagaagt gatcatgatg
    53941 atcaccggag acaacgtata ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag
    54001 cgtgttcctt caactgaatc gcaagggcgt atccatactt ccaccgcaac agttgtcgtg
    54061 atgccagaag ctgaagaagt agagatcgat attgaagaca aagatatccg tacagatatc
    54121 tatcatgctt caggtgctgg cggacagcac gtcaataaaa cagcatctgc tgtacgtttg
    54181 actcacttac caacagggat cgttgttgct atgcaagatg aacgttctca aatcaagaac
    54241 cgtgaaaaag caatgaaaat cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca
    54301 caaagcgagt atgatgccaa tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg
    54361 atccggacgt ataatttccc gcaaaatcgt gtgactgatc atcgtatcgg attaactatc
    54421 caaaaattag atcagatttt agcagggaaa ttagatgaga tcgttgatgc attgatcatg
    54481 tatgatcaaa catcaaaact ggaagctatg caaaatgggt aaaatgacct atcgagaagt
    54541 cctgtcacgg gcttcttctt ttttagagga tcatgggaaa gaagcatata gtatccaatt
    54601 tttatttttg gaacgaaaac aatggaaaaa acttgattgg ctccttcata tgaacgaaga
    54661 gatttcagaa gaagaacagc ggttgatcga aaccgatctg cgacttctgc ttgcagatca
    54721 tccacctcag tatttgctag gatatgcaga tttttatgat catcggttaa aggtaactga
    54781 agcaacactg atccctcgac cagaaacaga ggagttagtc gaatggtgtt tagacgagac
    54841 gcctggtgta ccgttagaag tgatagatat tgggacaggg actggagcaa ttgctattag
    54901 tttgaaagca gcaagaaaaa attggcatat atctgctgtg gatctttcag aagaagcatt
    54961 agaagtagcg aaagaaaatg ctcagaaaga aggaacgaaa atcagttttt atcatggaga
    55021 tacattagga ccagtaatgg atcagcaatt tgatgtgatt atttctaatc cgccgtacat
    55081 cagtcggaat gaatgggaac tgatggatga aagtgtccgg tcattcgaac ctaaaatggc
    55141 cctttttgct gagaacgatg gattagctat ttatgaaaaa atagcagttg aagcaccaag
    55201 cgtactaaaa tcaaatggaa agattttttt agaaatcgga tttcgccaag gtgaagcagt
    55261 aaaaaaaata tttcagcaag catttccaga taaaaaagtg gcgataaaaa aggatctttt
    55321 tggcaatgaa cgaatgatcc aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt
    55381 gtttttttat tataaaaaaa cacttatttg acaaggatgt aaacgacttt ttggtttatc
    55441 aacaagttta tccactattg acaaatagat tatccacaaa cagtggaaaa cttgaggata
    55501 aactcagaca aaataagaat gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac
    55561 gatatgatga aacagaattg caagaggcag ccgaagcact gaaagaagga gaacttgttg
    55621 cttttcctac cgaaacagta tacggattgg gcgccaacgc tcttttacca aacgctgtca
    55681 agaaagtgtt ttctgtaaaa gggagaccgc aagataatcc attaatcgtc catgtcgcct
    55741 cttttgaaca agtaaaagag tatgtggaca actttcatcc ggcagcagag aagattgtga
    55801 agaacttctg gcctggacca ttaactttga tatttaagat caaaaaagac actttgccct
    55861 cagttgtgac gggaggatta tccacagctg catttcgtat accggataac aaaaaaacac
    55921 tagaagtaat cgaactttcc ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa
    55981 aaccaagtcc aacgactgca gatcatgtat atcatgatct acaaggaaaa attaccggga
    56041 ttattgatga tggagctacg cgaattggtg tagaatccac tgttcttgat ttaagtgatc
    56101 caacagccat gcctatgatt ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag
    56161 tcatcgaatc tcctgttgct attgatcagc atcttgtaaa agaaaatgaa acaccaaaag
    56221 cccctgggat gaaatacaaa cactactcac ccgatacacg tgttctgatg gtcagagatg
    56281 gcgattggtc aactgcagtt caatgggcaa aaaataagaa gatccgtgca ggtgtcatag
    56341 caagccccga aattgcggat caagtgcgga cagatactgc agctgtttat atgtataacg
    56401 acaactcagt agaagcggcg gctaaaggat tgtttgcagg tctaagagga ttagatgaac
    56461 caacacttgg attagatttg atttttgtac aagtgtatcc agaaactgga ttggggaatg
    56521 cttacatgaa tcgcttgaaa aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg
    56581 gttattgtcg cattctttta gtatgataca atgctatcaa gtaaatcttc tatcagtgaa
    56641 cgaaaaacta agcgtaacta atcgtaatag aagagatacg aatatttggc aatttgtttt
    56701 tgatgggaaa aataaaattg taggaaaata aaattgtagg aaaataaaaa ggagctgttg
    56761 tttgtggtag attacaaaac gtttgaccct gatctttggg ctgcgatcgc caaagaagaa
    56821 gaaagacaag aacataattt ggagctgatt gcctctgaaa actttgtctc cgaagcagtg
    56881 atggctgcac aaggaagtat cttgacaaat aaatacgcag aaggttatcc agggcatcgc
    56941 tattatggtg ggtgtgaatt tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa
    57001 gaattattcg gagcgaaatt tgcgaacgtc cagcctcact ccggctctca agccaacacg
    57061 gctgcttatc tcgctttggt agaaccaggg gacacgatct taggaatgga tctttccgca
    57121 ggcggacatc taacacatgg atcaccagtc aacttcagtg gcaaaaccta tcactttgtt
    57181 gcttacggtg ttgatccgac aacagaagtt attgattata atgtggtacg tattttagct
    57241 agaaaacatc aaccaaaatt gatcgtagca ggtgccagtg cttacggacg aacgatcgac
    57301 tttgcgaaat tcagagaaat cgcagatgaa gtaggcgcaa aactgatggt agatatggca
    57361 catatcgcag gattagtagc agcaggactt catcctaatc cagttcctta tgcagatatc
    57421 acaacgacta ccactcataa aacattgcgt ggccctcgag gcggcatgat tttgacaaac
    57481 gatgaagcat tagcaaaaaa aataaatagt gcggtatttc ctggtattca aggcggccct
    57541 ctagagcatg tgattgcggg taaagcagta gcattcaaag aagcattaga tcctgcattt
    57601 aaagaatata gtgaacaaat catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa
    57661 gcaattggaa ccagagtcat ttcaggggcg acagataatc atctgatgtt gatcgatgtt
    57721 cgcgagttag gtataaatgg aaaagaagca gagagcattt tggacagtgt gaatatcact
    57781 gtaaataaaa actcgattcc ttttgaaaca ctaagtccat tcaaaacaag cggtattcga
    57841 atcggcacgc cagcaatcac tactcgcggg ttcaaagaag aagatgctgt aaaagtggct
    57901 gaactagttg tcaaagcatt acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa
    57961 acaggtgtac gcgaattaac tgaaaaattt ccgttgcata aaaaataaaa atttaaattc
    58021 cattagtaaa cagaaaagtt caatgttggg cttttctgtt tattttttgg gaaaacggat
    58081 tctttgacaa tatcggtgat gctctctagt atatgaggtg taattttagt cttcacacta
    58141 aatctcaggg aggaaaagtc atgactgatc gtatctcgtt agaaaaggaa gcagtagcat
    58201 tcagtgatgc aaatgcgcct catccgcgta tctatgaact agcaccggaa gaaggaagag
    58261 ttttactaga agaagtacaa gaatcacctg tgaacaaata ttccgtcgat attgaagact
    58321 tgatggtgga tactggcgaa tggggaaaac tcaatattcg tctcgtacga ccagaaggga
    58381 atatggataa attaccagtt attttttata ttcatggagc aggctgggtt tttggcagtg
    58441 ctcaaaccca tgataaattg atcagagaac ttgcggttcg tacgaattcc gtcgttgtct
    58501 ttccagaata tagtcgatca ccagaagcaa aatatccaac agcaattgaa caaaattatg
    58561 ctgtgttgca acagctgaat aaattagcca agtcaaaaaa tctggatctc gagaatctaa
    58621 tcgttgcagg agattcagta ggtggtaaca tggctacagt catgacgatc ttaacaaaag
    58681 aacgaaaggg cctcccaata tccaagcaat tgctgtatta tccagtcaca aacgctgatt
    58741 ttgatacaga ttcctatcat gaattcggag aaaactattt tttgacaaga gaaggaatga
    58801 tgtggttttg ggaccaatat acgacgaatg aagaagagcg tgctgaaatc acagcttcgc
    58861 ctttgagagc aaatataaat gaactaaatg atcttccttc agcaatgatt ttaacgggag
    58921 aagcggatgt attaagagat gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg
    58981 ttcctgttac acaagtacgc ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta
    59041 tggatcagac aaaggcaacg cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa
    59101 aagaataagc tttgcttgtc cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa
    59161 aaatggcgag tttgactagt caatcacttg attttgtttt acaattaaaa agaaacgaca
    59221 gaacaaagga gagcttaaaa aatgggcaaa tttcaagtga tcgatcatcc attgattcaa
    59281 cacaaactaa ctattattcg ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc
    59341 gatgaaatcg ctatgttgat ggcatatgaa gtatcacggg atatgccatt ggaagacatc
    59401 gtgatcgaaa caccgatcac tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg
    59461 attattccaa tcttgcgcgc aggtattgga atggttgacg gtatcttgga attgattcca
    59521 gctgctaaag tgggacatat cggtctttat cgagatgaag aaacattgga accacatgaa
    59581 tatttcgtca aacttccaga agacattgat gcacgtcagt tgtttgtagt agatccaatg
    59641 ttggcaacag gcggatcagc aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg
    59701 aacattaaat ttgtttgttt ggtagcagca cctgagggag taaaagcttt gcaagaagcg
    59761 catcctgata ttgacatcta tacagcttct ctagatgaaa aattgaatga acatggttat
    59821 atcgttcctg gactagggga tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg
    59881 aaaaagggtg tgacacaact tttgtcacat ccttttttgg tcatttttaa cgtttgatgc
    59941 agaacagctt tttcacagct ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga
    60001 tggtcaaatt caaatctttt ttagttcttt gtagttaacg attactacac ccttactttg
    60061 cgtcatcaag ttcgcatttt ctgcagtttc atcaatctca acaacagcgg aatttttcaa
    60121 ttgctttgaa atcgttccag tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt
    60181 gatcttgaat ttttcaccaa tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt
    60241 tgccattttc ataaaacctg cctttcacat caattatacc acgttttatg aaaaatatca
    60301 tgaaattaaa ctaattttcc atcggactct ttacaatcag ccaaatagag tatataattt
    60361 cttttggata aggccagctt agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc
    60421 gtaggttcga tccctatagc tggcattaaa agagaagaca gatatatcaa ggatttttaa
    60481 tccattgata catctgtctt tttgtttaaa ggaaataaag tatgctttct tagcaataga
    60541 gcatgtatta tttctataaa aatttaggag aataaaacga ctagcaatat agctgtgaaa
    60601 tcaaatatca catgaactgc ccaacttgta atgatcgagt tgcttttaat ggcagcttgg
    60661 ttgaataaca atcttgccac cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata
    60721 tttccattat cataagtact gtaatggata aatccaaaga tgattgctga caagaaacta
    60781 gcgacaggaa tgctgaactt actagaaaaa atagcgagaa aatacactga gaacaattct
    60841 tctccaagta tcatgatagg caaaataata aataataacg ggctgaaagc ctcatttatt
    60901 ggattgcctg ttaaacccca tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg
    60961 attactacat tgataaaaaa atatttggct atatttttga caggagcgac aggtttcgag
    61021 aacatttttt tcgtatcttt tttaccaaaa acaagtgtaa atgctataaa tccagtgata
    61081 gttaaagaaa aaatgctaat aatggatggc gcaaatagca accctaacaa accgagtaca
    61141 acgacaatag tgcccaatat ccctttttta agttctattt tttttcctaa aatgttattc
    61201 atgataataa ctcctactat tcagtttttg aagatagagt cagtatacat gaagagttaa
    61261 aataggcaat gtttttttat ggatatttgt attgctaaaa atttatctta tatattcatt
    61321 ttgattatac tacttttatt tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat
    61381 taagcgtaca attttttaga tgttccgagc gtacttcaat catttcaaag gagttgctac
    61441 ttaaatcgat attttttaaa tcagtatcaa aaaagctagc attttctaaa tcattagtag
    61501 aaaacattat tttagtgagg gaacagtcta caaaatctgc gcttgataag acagagtgct
    61561 caaaggtact ttcttttatg tttgaaccag ataaatttac atagtccatt ttacaattct
    61621 cgaataacgt gttcccaata taactttcca tcaatttagt acctgttaat ttacaattga
    61681 caaatttcac gcgatgcaac atgctttttg tcaatataca gcctgaaaaa tcacagtttt
    61741 cgaatataca gtcggtaatc tccattcttt gaaaatcatt ggaagcaaaa aatgcatact
    61801 tgaaaataga tttgtaaata attggatggc agattatttg gttttcgatg acctcttttt
    61861 gtgaaaggat attatacagt tcaaaatctt cgttttcata gatatgaaaa aaggatcctt
    61921 tattcaagct tgcttcatta actttaggaa aaatcatatt taattacctc cattttcata
    61981 aagtcatatt aacaaaagct aaaccaactt acattagcag aaaatgagat aggttttcgc
    62041 ctttttcgat aacgatagtg tcgctatctc ttgaaacgtt cttagaattg ccctgagaga
    62101 cacaaattag gcgtaattct aataaaaatc ttagaataaa aatttttact gatttaattt
    62161 aga
//
LOCUS       DAYYAJ010000003        54562 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000003 DAYYAJ010000000
VERSION     DAYYAJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.003"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..643)
                     /locus_tag="ACHATZ_000146"
     CDS             complement(65..643)
                     /locus_tag="ACHATZ_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8402709.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(720..1250)
                     /locus_tag="ACHATZ_000147"
     CDS             complement(720..1250)
                     /locus_tag="ACHATZ_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8402710.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1254..2114)
                     /gene="rlmB"
                     /locus_tag="ACHATZ_000148"
     CDS             complement(1254..2114)
                     /gene="rlmB"
                     /locus_tag="ACHATZ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8402711.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2107..2502)
                     /locus_tag="ACHATZ_000149"
     CDS             complement(2107..2502)
                     /locus_tag="ACHATZ_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8402712.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2499..3911)
                     /gene="cysS"
                     /locus_tag="ACHATZ_000150"
     CDS             complement(2499..3911)
                     /gene="cysS"
                     /locus_tag="ACHATZ_000150"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8402713.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3908..4444)
                     /gene="epsC"
                     /locus_tag="ACHATZ_000151"
     CDS             complement(3908..4444)
                     /gene="epsC"
                     /locus_tag="ACHATZ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8402714.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4530..4769)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4766..6226)
                     /gene="gltX"
                     /locus_tag="ACHATZ_000152"
     CDS             complement(4766..6226)
                     /gene="gltX"
                     /locus_tag="ACHATZ_000152"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8402715.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6396..7553)
                     /locus_tag="ACHATZ_000153"
     CDS             complement(6396..7553)
                     /locus_tag="ACHATZ_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8402716.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7587..8957)
                     /gene="radA"
                     /locus_tag="ACHATZ_000154"
     CDS             complement(7587..8957)
                     /gene="radA"
                     /locus_tag="ACHATZ_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8402717.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9020..9547)
                     /locus_tag="ACHATZ_000155"
     CDS             complement(9020..9547)
                     /locus_tag="ACHATZ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8402718.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9777..10583
                     /gene="proB"
                     /locus_tag="ACHATZ_000156"
     CDS             9777..10583
                     /gene="proB"
                     /locus_tag="ACHATZ_000156"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8402719.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10580..11827
                     /locus_tag="ACHATZ_000157"
     CDS             10580..11827
                     /locus_tag="ACHATZ_000157"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8402720.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11903..12247
                     /locus_tag="ACHATZ_000158"
     CDS             11903..12247
                     /locus_tag="ACHATZ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402721.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12364..12690)
                     /locus_tag="ACHATZ_000159"
     CDS             complement(12364..12690)
                     /locus_tag="ACHATZ_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402722.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13305..14507)
                     /locus_tag="ACHATZ_000160"
     CDS             complement(13305..14507)
                     /locus_tag="ACHATZ_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8402723.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14624..15139
                     /locus_tag="ACHATZ_000161"
     CDS             14624..15139
                     /locus_tag="ACHATZ_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8402724.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15338..15784)
                     /locus_tag="ACHATZ_000162"
     CDS             complement(15338..15784)
                     /locus_tag="ACHATZ_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8402725.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15831..17309)
                     /gene="msr(C)"
                     /locus_tag="ACHATZ_000163"
     CDS             complement(15831..17309)
                     /gene="msr(C)"
                     /locus_tag="ACHATZ_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8402726.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17663..18292)
                     /locus_tag="ACHATZ_000164"
     CDS             complement(17663..18292)
                     /locus_tag="ACHATZ_000164"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8402727.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18276..18740)
                     /locus_tag="ACHATZ_000165"
     CDS             complement(18276..18740)
                     /locus_tag="ACHATZ_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8402728.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19227..20495)
                     /locus_tag="ACHATZ_000166"
     CDS             complement(19227..20495)
                     /locus_tag="ACHATZ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8402729.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20726..21463)
                     /locus_tag="ACHATZ_000167"
     CDS             complement(20726..21463)
                     /locus_tag="ACHATZ_000167"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8402730.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21530..22099)
                     /locus_tag="ACHATZ_000168"
     CDS             complement(21530..22099)
                     /locus_tag="ACHATZ_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8402731.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22227..23099
                     /locus_tag="ACHATZ_000169"
     CDS             22227..23099
                     /locus_tag="ACHATZ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8402732.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23441..24313
                     /locus_tag="ACHATZ_000170"
     CDS             23441..24313
                     /locus_tag="ACHATZ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8402733.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24310..26268
                     /gene="nagE"
                     /locus_tag="ACHATZ_000171"
     CDS             24310..26268
                     /gene="nagE"
                     /locus_tag="ACHATZ_000171"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8402734.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26600..27451)
                     /locus_tag="ACHATZ_000172"
     CDS             complement(26600..27451)
                     /locus_tag="ACHATZ_000172"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8402735.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27574..28041
                     /locus_tag="ACHATZ_000173"
     CDS             27574..28041
                     /locus_tag="ACHATZ_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8402736.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28304..28786)
                     /locus_tag="ACHATZ_000174"
     CDS             complement(28304..28786)
                     /locus_tag="ACHATZ_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8402737.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28886..29749)
                     /locus_tag="ACHATZ_000175"
     CDS             complement(28886..29749)
                     /locus_tag="ACHATZ_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8402738.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30347..31222
                     /locus_tag="ACHATZ_000176"
     CDS             30347..31222
                     /locus_tag="ACHATZ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8402739.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31367..31519)
                     /locus_tag="ACHATZ_000177"
                     /pseudo
     CDS             complement(31367..31519)
                     /locus_tag="ACHATZ_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31787..33118)
                     /locus_tag="ACHATZ_000178"
     CDS             complement(31787..33118)
                     /locus_tag="ACHATZ_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8402740.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33267..33980)
                     /locus_tag="ACHATZ_000179"
     CDS             complement(33267..33980)
                     /locus_tag="ACHATZ_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8402741.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            34155..35579
                     /locus_tag="ACHATZ_000180"
     CDS             34155..35579
                     /locus_tag="ACHATZ_000180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8402742.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            35878..36585
                     /locus_tag="ACHATZ_000181"
     CDS             35878..36585
                     /locus_tag="ACHATZ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8402743.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            36572..36895
                     /locus_tag="ACHATZ_000182"
     CDS             36572..36895
                     /locus_tag="ACHATZ_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8402744.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(36984..38021)
                     /locus_tag="ACHATZ_000183"
     CDS             complement(36984..38021)
                     /locus_tag="ACHATZ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8402745.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(38342..39325)
                     /locus_tag="ACHATZ_000184"
     CDS             complement(38342..39325)
                     /locus_tag="ACHATZ_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8402746.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(39740..40510)
                     /locus_tag="ACHATZ_000185"
     CDS             complement(39740..40510)
                     /locus_tag="ACHATZ_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402747.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40503..41438)
                     /locus_tag="ACHATZ_000186"
     CDS             complement(40503..41438)
                     /locus_tag="ACHATZ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402748.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(41754..43403)
                     /locus_tag="ACHATZ_000187"
     CDS             complement(41754..43403)
                     /locus_tag="ACHATZ_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8402749.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGSSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(43520..46123)
                     /locus_tag="ACHATZ_000188"
     CDS             complement(43520..46123)
                     /locus_tag="ACHATZ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8402750.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(46135..47184)
                     /locus_tag="ACHATZ_000189"
     CDS             complement(46135..47184)
                     /locus_tag="ACHATZ_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8402751.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(47210..47524)
                     /locus_tag="ACHATZ_000190"
     CDS             complement(47210..47524)
                     /locus_tag="ACHATZ_000190"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8402752.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(47540..47986)
                     /locus_tag="ACHATZ_000191"
     CDS             complement(47540..47986)
                     /locus_tag="ACHATZ_000191"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8402753.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(47987..49909)
                     /locus_tag="ACHATZ_000192"
     CDS             complement(47987..49909)
                     /locus_tag="ACHATZ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8402754.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(50121..51017)
                     /locus_tag="ACHATZ_000193"
     CDS             complement(50121..51017)
                     /locus_tag="ACHATZ_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8402755.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(51010..51690)
                     /locus_tag="ACHATZ_000194"
     CDS             complement(51010..51690)
                     /locus_tag="ACHATZ_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8402756.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(51691..52473)
                     /locus_tag="ACHATZ_000195"
     CDS             complement(51691..52473)
                     /locus_tag="ACHATZ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8402757.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(52466..53383)
                     /locus_tag="ACHATZ_000196"
     CDS             complement(52466..53383)
                     /locus_tag="ACHATZ_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8402758.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(53547..53801)
                     /locus_tag="ACHATZ_000197"
     CDS             complement(53547..53801)
                     /locus_tag="ACHATZ_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402759.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT        16027 a        11314 c         9467 g        17754 t
ORIGIN      
        1 tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt aagatttttc
       61 taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt actctcccgt
      121 aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct acaaaatcaa
      181 cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg atttcgcaat
      241 tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc aatgtgatct
      301 ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact tcatcttttt
      361 gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga ttgcttataa
      421 cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt ctcaatgcct
      481 gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt cttaaataat
      541 aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca gcaaatgctt
      601 cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa ttcctccata
      661 aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag aacgatatgt
      721 tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa ctgctcttca
      781 ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc ttgggctatt
      841 gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg taatgctcct
      901 tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac ttgtgtcaaa
      961 cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc gtcttctttc
     1021 gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg aactagttgg
     1081 gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga taaacgatgt
     1141 aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac aagttccggc
     1201 cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat ggtctactcc
     1261 ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact ggcatttaag
     1321 ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt tttcaatcct
     1381 ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt ggcattccat
     1441 ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt ttctttgagc
     1501 tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc gactgcccca
     1561 gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg tatgatcaca
     1621 ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg tggatcttcg
     1681 atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg ttcaatcagt
     1741 tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg atgcacgccg
     1801 tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac agcttgttca
     1861 cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg aagaaagagt
     1921 ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa gacgaaatta
     1981 tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt ttgcttttta
     2041 tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg tttgtttgtt
     2101 ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca attcttccat
     2161 ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc ctgttgcgac
     2221 ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat ttcttcctct
     2281 gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt gctgaatcaa
     2341 aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct tattgggttt
     2401 tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg catctccaac
     2461 ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct tctccatcgg
     2521 gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc acgaatttcg
     2581 tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat caattgttca
     2641 atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga aagccattgc
     2701 gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg gtctagataa
     2761 gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc attaaaatca
     2821 tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat tttatccgca
     2881 tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc aaacgtattc
     2941 ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta gcggatcgga
     3001 cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac gtttttgatc
     3061 atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc gtcttcgcca
     3121 atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt tttcgcttca
     3181 ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc accatggata
     3241 tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg ccaaccggga
     3301 cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg tttccataaa
     3361 gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg acttgctccg
     3421 acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa tttacgggta
     3481 cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa cacttgaata
     3541 aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc aggttcgaca
     3601 ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac ttctggtgct
     3661 gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt gaaattcgat
     3721 acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc gaaagcaatg
     3781 gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca gacatacata
     3841 ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa tgtattgtag
     3901 atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact tttgctggta
     3961 ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct gcaccgattt
     4021 tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag agcatggctc
     4081 cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct aatgttacgc
     4141 catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg attcccattc
     4201 catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca acaccagtta
     4261 ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc tgatataaaa
     4321 aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa acgatctcag
     4381 ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg gtaaaccaac
     4441 tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc ttataaagaa
     4501 aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg gttccacttt
     4561 tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct tggctacttt
     4621 gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact cccagaggca
     4681 tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta atgatcagct
     4741 gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt gctaatgatt
     4801 tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt tgtcctgata
     4861 cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct ttttgaacag
     4921 ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct agctgtgctt
     4981 tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt gctgcttcat
     5041 tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct gcagcatagc
     5101 tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc ttttcatctg
     5161 ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca gtaaatgcat
     5221 ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc gcatcgaaag
     5281 ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct tggtgttcga
     5341 agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg aacattgcat
     5401 ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat tcgtcacgtt
     5461 tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg aaggttggtg
     5521 gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca atatggtcat
     5581 ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca gcaaaattat
     5641 aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta tcggattcga
     5701 acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga ggcacacgga
     5761 aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca ggtgtcaagt
     5821 tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct tcccgttctg
     5881 cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca agcagctgat
     5941 caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg tattctccag
     6001 gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc aactggcttt
     6061 tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata ataaagtctc
     6121 catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt ccaatatgca
     6181 ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc ttcctcttct
     6241 ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct caaatatcat
     6301 ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta aagccaactg
     6361 gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt agtctttttc
     6421 tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc aaagatcatg
     6481 cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc attcatgtaa
     6541 tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac tccttgttgg
     6601 cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat gacgactggt
     6661 tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa ttcagagact
     6721 ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc caatttgatc
     6781 aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc gacagatata
     6841 ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg cacacgtttc
     6901 aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt agggatcatc
     6961 aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc aatgatcacg
     7021 cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc taacagttga
     7081 ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc aaaaactttc
     7141 ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa gatgatcatc
     7201 agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag aggggtcgag
     7261 atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag aagataagtc
     7321 aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat atgtttggcc
     7381 aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt aaatgtatta
     7441 ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa aaatgaaata
     7501 cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg catcgttttt
     7561 cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt ttcagccaaa
     7621 gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa gttgttttta
     7681 ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg ttcaatatga
     7741 ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc cgttggctgt
     7801 gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc aatcgctggt
     7861 tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg aagaagcaac
     7921 ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc aagacctgtt
     7981 gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat ctctaccaaa
     8041 attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc gtctaggcgt
     8101 tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc atgcatttca
     8161 aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa aatccgaaaa
     8221 ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc taacattctt
     8281 gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc tatcccattt
     8341 gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac acttcctgcc
     8401 acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac ataatcaggg
     8461 gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta tagataaaat
     8521 tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc gctttcttcg
     8581 ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac ttgtaacagc
     8641 aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc tggcaccaca
     8701 cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg ttctttttta
     8761 gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact tgtccgagtt
     8821 ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt tccgcaattt
     8881 gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac aaattgtact
     8941 tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca ttaaatcaag
     9001 cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga cgttgagcag
     9061 tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg atacgctcgc
     9121 cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg atatggcctt
     9181 catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt gtcaacacta
     9241 aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca tcttctccca
     9301 tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca ttttctgata
     9361 catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct gctttccaga
     9421 tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca tgatgtgtag
     9481 cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca aatcctcttt
     9541 ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc gaggctgtaa
     9601 aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac cattaattcg
     9661 ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg actttactcg
     9721 tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg tgtcacatgc
     9781 gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt acattgattt
     9841 atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc ctttctgatc
     9901 ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc gttggtatgc
     9961 ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct gcggcggctg
    10021 ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat aatcaacaga
    10081 cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt aaaaatgtga
    10141 ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac gaaaacgata
    10201 ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat cagctgtcag
    10261 ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat atcgacgggt
    10321 tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat atcgataaaa
    10381 tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga acaggcggta
    10441 tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca atgattttag
    10501 caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa gtaggtactt
    10561 tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg caaaagatag
    10621 cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg ttcaaatggc
    10681 acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag acttagaaaa
    10741 agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga cacctgagcg
    10801 aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc ctattggaaa
    10861 agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc gcgtgccact
    10921 aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg cagctagtct
    10981 agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat tcttctcgaa
    11041 tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat ctccttatgc
    11101 catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc gattgacaga
    11161 atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag tcaaagaaca
    11221 ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata tcgataaaga
    11281 agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc gtccatctgt
    11341 ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt tcttgccagc
    11401 tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc gtgcgttaga
    11461 atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat atctagatta
    11521 tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata tcaatcggca
    11581 taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc aacgtttctt
    11641 aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta cagacggatc
    11701 ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg cccgtggtcc
    11761 aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg gacaaatccg
    11821 cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg taaaggttct
    11881 atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta tttaccttat
    11941 ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct ttcaattcaa
    12001 aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca aaatcgggca
    12061 aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct tattagcgac
    12121 aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc tactgattgc
    12181 tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga atcggacttt
    12241 ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa aaactgccgg
    12301 actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat aaacggcgtt
    12361 cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa atccgtcaaa
    12421 tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg ttttttcttt
    12481 ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca taccagctat
    12541 tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca attctttgat
    12601 atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa cgagtgtcaa
    12661 cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt ttcttaaatg
    12721 aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa aaagggaatg
    12781 tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct gccaactcct
    12841 caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat tgttccttgt
    12901 gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa tcagctgacc
    12961 ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt ttgtttctct
    13021 aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca atcagctgtc
    13081 cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt ttgtttctct
    13141 aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc aaaagcttgc
    13201 atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg tttggctgtt
    13261 cttatgactt gatgcaggac agctttttca caacctctga tttcttattc ttcttcattt
    13321 gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac aatggcacct
    13381 gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc aataaatggt
    13441 gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac gtttcgttgc
    13501 agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc ttggaaaaat
    13561 cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc tcctaataac
    13621 agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt cgtaaacttc
    13681 cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc ccagccaaaa
    13741 aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc atacgcaagt
    13801 aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg aagtttatga
    13861 ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt ctcagtttca
    13921 ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac cccaaaaaat
    13981 ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac agccattccg
    14041 cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac taaaatactg
    14101 acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca gccggcacgt
    14161 aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa tacaacgatg
    14221 ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa gaaacctgta
    14281 aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt cgtttcagct
    14341 ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag acccactccc
    14401 ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc ctttgtttct
    14461 tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc tttctctcta
    14521 ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg tggaaacttt
    14581 actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata ctttagccta
    14641 tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga agcagctgat
    14701 cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca agttagacca
    14761 gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga tctaccactt
    14821 gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc ccgctcctac
    14881 gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata aaacaacagc
    14941 tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa
    15001 aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg ttgtgatcca
    15061 gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa gagaacaaat
    15121 gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta ttgaagagca
    15181 aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg aacgacaggc
    15241 caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac tatgacaaaa
    15301 agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt tgaattgctt
    15361 ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa gcggcaatca
    15421 cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt ccttgttcca
    15481 tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag catgaaatag
    15541 ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca atttttttcg
    15601 ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca ggcactagac
    15661 caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac gcgctcatcc
    15721 ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt ttatttgtag
    15781 gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt
    15841 ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc agccgtacgc
    15901 tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat ttgcattagc
    15961 ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc taacagcaac
    16021 acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc tcccccactt
    16081 aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta
    16141 cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat ggattcttca
    16201 gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg agagagaagg
    16261 attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga accattttca
    16321 ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac cagtaatgtt
    16381 ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct gtggagttca
    16441 agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt tgtttctcct
    16501 attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt ttgtacactg
    16561 tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt ctttttactg
    16621 gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt
    16681 tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc caattccttt
    16741 tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc ttttacttcc
    16801 cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa aaccagcgta
    16861 ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc tagatgggtt
    16921 gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat caactcgatc
    16981 tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct taactggaaa
    17041 tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa ttgaggcagg
    17101 tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc accggctatt
    17161 agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat acgatcttgc
    17221 tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct gacttgtaga
    17281 tttgttatat ttactgctaa attttccata tgttttacac tcctatttca tgtaaaacaa
    17341 caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg catcacatct
    17401 tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata gctttcacct
    17461 attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg tttatacatg
    17521 tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat cgatgcagtc
    17581 atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa gcaatagcta
    17641 ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt gctaaacgaa
    17701 cagactcgta accttcttct accgcttttc ctattgataa aataataact ggaacataac
    17761 ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca ccaattggat
    17821 ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct aagctagaat
    17881 cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa ggaatgatcg
    17941 cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa gcttggttgt
    18001 agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc attgcagaag
    18061 aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct tttgcttcct
    18121 cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt gcagtcgtcg
    18181 cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc
    18241 gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta
    18301 acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt
    18361 agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta
    18421 tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc tacatacact
    18481 tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc
    18541 agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc
    18601 aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata
    18661 ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga
    18721 ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac
    18781 ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa
    18841 cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga
    18901 taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc
    18961 tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat
    19021 cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa
    19081 gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa
    19141 gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg
    19201 cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc
    19261 cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca
    19321 gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt
    19381 agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg
    19441 taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt
    19501 gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt tttttctccg
    19561 gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa
    19621 gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat
    19681 gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg
    19741 taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa
    19801 gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta
    19861 aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca
    19921 gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa
    19981 gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag
    20041 tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga
    20101 gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa
    20161 tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct
    20221 gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct
    20281 gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt agctgactgc
    20341 aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt ctgagttgcg
    20401 acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag atttcctaaa
    20461 agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg aattatgaaa
    20521 cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat
    20581 aacatttgtt tctttttaat aaatttgctt tttctgcata aaaagacgta aatgaagaat
    20641 attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt
    20701 ctctctggat attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt
    20761 tgctgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt
    20821 cagatgttaa tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc
    20881 tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt
    20941 tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc
    21001 cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga
    21061 tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac
    21121 ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag
    21181 cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg
    21241 ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata
    21301 gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta
    21361 taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg
    21421 aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt
    21481 ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc
    21541 gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat
    21601 ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca
    21661 gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca
    21721 agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac
    21781 caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat
    21841 tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca
    21901 gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat
    21961 ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tccgcttgga
    22021 tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt
    22081 tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt
    22141 atgatgtcta atacttataa aacatactta actattcctt ttaactatac ttagtggtat
    22201 gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag
    22261 tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc
    22321 tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc actcgaggct
    22381 cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct
    22441 tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa
    22501 tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt
    22561 tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca
    22621 tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac
    22681 aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgga
    22741 atcatccttt agcaaagcaa acagtcatcc atccgcagga actgaaaact gttcctttga
    22801 tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag
    22861 atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata
    22921 acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaag
    22981 tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc
    23041 agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag
    23101 tagagttttt ttccttcgct ttcagctttt taacaacgaa taagttaagc aaaagatcca
    23161 atgattaaat aacataagtt tcttaatttg ttccttcctt ttatttctga taattccagt
    23221 aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt
    23281 acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg
    23341 aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc
    23401 tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt
    23461 taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg
    23521 gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt
    23581 tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg
    23641 ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt
    23701 gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa
    23761 taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga
    23821 attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac
    23881 ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa
    23941 taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc
    24001 agaattggaa acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa
    24061 tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga
    24121 aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg
    24181 tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc
    24241 catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga
    24301 ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg
    24361 gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac
    24421 gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg
    24481 ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg
    24541 gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact
    24601 ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt
    24661 attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa
    24721 ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt
    24781 gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta
    24841 gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc
    24901 ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt
    24961 gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta
    25021 acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca
    25081 tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgtta
    25141 gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt ctcatttatg
    25201 ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg
    25261 agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc
    25321 ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta
    25381 acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact
    25441 cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat
    25501 aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact
    25561 tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat
    25621 caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg
    25681 caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt
    25741 ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt
    25801 ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa
    25861 gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa
    25921 aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat
    25981 gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact
    26041 gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa agtcgcaaga
    26101 ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat
    26161 atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt
    26221 gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc
    26281 cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct
    26341 ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agccaaacgt
    26401 aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt
    26461 ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt caaatttatg
    26521 caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
    26581 ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
    26641 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
    26701 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
    26761 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tttttacctg
    26821 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
    26881 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
    26941 tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
    27001 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
    27061 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatacactga
    27121 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
    27181 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
    27241 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
    27301 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
    27361 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
    27421 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
    27481 taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
    27541 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
    27601 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
    27661 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
    27721 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
    27781 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
    27841 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
    27901 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
    27961 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
    28021 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
    28081 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
    28141 taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg
    28201 accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct
    28261 ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta
    28321 ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat
    28381 ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat
    28441 tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca acaagatacg
    28501 ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc gtttgaacac
    28561 cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt
    28621 cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt
    28681 gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac
    28741 ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt
    28801 ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt
    28861 ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac
    28921 tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta
    28981 actcataatc tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag
    29041 tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca
    29101 tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac
    29161 tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt acttgatttg
    29221 gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta
    29281 acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg
    29341 ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat
    29401 caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg atttctgaag
    29461 gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat
    29521 atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta gccaaagcca
    29581 atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt
    29641 ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg
    29701 tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc
    29761 tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac
    29821 caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt
    29881 cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt
    29941 ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat
    30001 ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct
    30061 tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct
    30121 aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa
    30181 cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt
    30241 gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt
    30301 ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct
    30361 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
    30421 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
    30481 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
    30541 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
    30601 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
    30661 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta tcgaacgctt
    30721 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
    30781 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
    30841 gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg cctatcttcc
    30901 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
    30961 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
    31021 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
    31081 tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag actttcggga
    31141 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa
    31201 agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa
    31261 tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa
    31321 cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac
    31381 aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta tgatactttt
    31441 ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc tgacaacaac
    31501 tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaataa cgattaataa
    31561 tattaacagt ctttattttt caccaccaaa aacctatctt tgcatatact aaatagacgt
    31621 atatacacag ataggtttgt cttgtgaaaa ataatgatta tccttaccaa aaaatgtttg
    31681 tttttctaca tgttcgtact tatcaaaaaa tatgatcctt attgaaatta ctggctattt
    31741 atattccaac gacttcttgt gttaattttt gttatctcat agtaaattat gtttcatttt
    31801 cacttaattt agttgtcacg tgttcgtcaa ttcgtaaaaa cggtaaatat ataaacacac
    31861 ttgcggctat aattaatatt tgtaaaacag ctgctcgcca gtctccggct gttgctaaaa
    31921 atcccgatat aactggtggc gtagtccaag gaatttgcac tactacatga ttaattaatc
    31981 ccacagcagt tgcaaaatat gctactgtca aagaaaaaat cggtgcaagc acaaagggaa
    32041 taatcagact gatattaaat acaataggca atccaaaaat aataggttcg cttatattga
    32101 atatagcggg agttaccgat aatttagcaa agtccttata atcttttcta cgactgaaga
    32161 taaatattgc aatcaataaa gcaatcgtat ttcccgatcc acccattacc gcaaaggtat
    32221 ctttaaaggc catattgata atatgtggaa tttgctcttt gtttgcataa gctgccatat
    32281 tttcttgcat attctgaatc aaaaacggat ctaacaatga acctgatatt actgcttgat
    32341 ggataccaaa tccaaaaaat agattagcaa tagatgtaat taacaaaaat cctggtaaac
    32401 cagttgtaat tttacttaat ggctctgtaa ctatcgtggt aattagctta tttatatcca
    32461 tattaaacaa ttgaatgatt ataaatgaca ataaagcaaa cgaggtaatc gtaataatga
    32521 ttgggattaa tacattaaaa gactttaaaa cagcgggagg aatatttccg gatagctgaa
    32581 tctgtagttt cttattatta gaaagtttaa taaataattc tgtagccgct aatccaacaa
    32641 atatacctac aaacattcct gaagctcctg tataggatat aggtatcaca cccgtcactt
    32701 caacagcttt atccgcattt tcaggagtaa acatcatctg cataggtgtt aatacaacaa
    32761 atgtcgaaat agcaactaat accgctgcaa tatgatttgt ataatttctg cttgcacaca
    32821 tatgataaga tactgctgct acaactaata atgtagttat gcttaatgta ccattggcaa
    32881 ttgaagtacc aatttgttgt aacgtggtca atgtactagt atcgacaatt tcccccatga
    32941 atcctgttgg ttctaagatc actgaattaa taagtgtcac aaatcctgct aacatcataa
    33001 atggcataaa tgtagcaaac gcatctcgca aagttcttaa atggatctga ttccccaact
    33061 tagtcgcaac tataccaaat ttttcaaata caatgtcctt cctgctactt ttgcccatat
    33121 tttttctcct ttatgcttat tgtaatcgat tactttttcc acccaaataa tacatcactt
    33181 atcaaaaaaa gtaaatactt ttaattaaat atatatatct tttttagtat aaaaaaataa
    33241 gctctgaaaa aataattttc ccgagcttat ttttcaacaa aaacagcttc aaaaacaaag
    33301 tcttgatata aatgtctcga taccgaatac tcgaaaggtt taccagacga tagagcaaca
    33361 actttgtcta tctcaatcat aaattcacct ggatctattt ttaatatttt tgcgtcaaat
    33421 tcattcgcag tatctccttt gatacgtatg tgcgaagtct ttatttccag acccagttca
    33481 tttgtgatat aagcatatac agatttcttt aaatgttttg gttccaagcc tggtatgact
    33541 gctaatggca tgaatgtttg ctcaatcgag tatggttgac aatcaataaa acggcttcgt
    33601 acaatattat atacatagtc ttttacactg atacttaatt tatcagctac ttttttcgat
    33661 ggaagttcaa ttgagaaagt atggatttct gaatctacgt gttcttcacc aaccacgcta
    33721 aacaaacctt cgtttggacc aagaactcgt gagttatcaa ttggtgttct gacgacttca
    33781 gtcccatacc cacttctact tcgcaaaact ccttcggaaa caagtaagtc tacgcctttc
    33841 tttactgtta acttgcttgt atgataaata actgccaatt catctcctgt aggaagtttc
    33901 tcaccaattt tatactctcc atttacaatt tttttcttta tatctaagta aatttccata
    33961 tacttcgcaa tttttttcaa tggtgaccct ccaatcaaaa acatatcttc atctatactt
    34021 taacataaat aaaccgataa aatccacatc aaaattatac acacaataaa aaagatatat
    34081 atatttaaac aaaactattt actttaagtt gtaagcggtg ttatcattaa gtggaaacag
    34141 gaggtaaaaa aattatgaca actatgaaaa agctaacttt tccagatgat ttttggtggg
    34201 gttccgcttg gtcagctgag caagcagaag gaaaaggaga aacaggaaaa gcagagacta
    34261 tctgggatcg ctggtttaaa gaacaacctt accggtttta tcaacgaatc gattcctcta
    34321 ttacaaccga tcacatccat caatataaag aagatataga actaatgaaa aaaactgggc
    34381 ataactcctt tcgcgtttct atttcttggg cacgaatgtt tccaaatggc ggtaccggaa
    34441 aagtaaatcc caaagccatt tctttctatc gaaaattatt ttccgaaatg aataataacg
    34501 gaatcaaagt atttgccaac ttatatcatt ttgacatgcc tgctactctt caagataaag
    34561 gaggatggga atcacgtgaa gtagttgatg cttatgttca ttacgctgaa acttgtttta
    34621 aagaatttgg tgacttagtt tgtcattggt ttaccttcaa tgaaccactt gggcctattt
    34681 taggtactta tcttgaagat tttcattatc caaacctcat tgactttaaa cgcggagcgc
    34741 aagcagcatt cttcactata cttgcacacg caaaagcaat acaagtattt aaagaactag
    34801 ctcttcctag taaaataggc gttattctaa atttgagtcc tacttttcct cgtagtcaaa
    34861 atcctgctga tgtgaaagca gcagaaatcg ctgatttatt ttatacgcgg agctttttag
    34921 atcctatggt taaagggaat ttttctaaaa agcttatcct gttattacaa gaatataatc
    34981 agcttcctga agattatacg aaagaagatc ttgtatgtat ctacgaaaat accgctcaaa
    35041 ttcttggact aaactattac gagccacgtc gcgtcaaatc tcgttcttct atggtggata
    35101 catctagtcc atttttacca gagtggtttt tcgaaccaca tatcatgcct ggacgacgaa
    35161 tgaacgaata ccggggatgg gaaatttacg aaaaaggaat ttatgatttg tgcatggata
    35221 tcaagaacca ttatggaaac atcgaagcat ttatctcgga gaacggaatg ggcgttgcgg
    35281 atgaagaacg tttcatggat gatacagggc aaataattga tgattatcgg attgagtata
    35341 tcaaagatca tcttgcttac ttatggaagg caataaatga aggatgtaac attaaggggt
    35401 atcatctttg gacatttatt gattgctggt catggatcaa cgcctataaa aaccgatatg
    35461 ggctggtttc actcgacctt actactcaaa aaagaacaat taaaaaaagt ggcgaatttt
    35521 acaagcaatt aagtgataca aatggattta gatacgatgt agacttgtta gttgaataat
    35581 attctaaaat tccacttgaa gtgaacacat tagaaaacaa gaataatgag aaataccaat
    35641 aacaaatact ttttcttcat acttcattta tacttgtcag tataagagac tgtgacaaat
    35701 gtagtgatcc tcagaagtta ggtctggaaa tctaacttga gaagattttt ttaaactacg
    35761 tccagtcttt tttatttgtt taaaattgaa gttgacaagt cttcattaaa aatttagaat
    35821 attcacaagt ttgacgacaa ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg
    35881 gatgaaaagt tagatataaa aactgcgatc aaagacacgt tacctaccgt ttttggttat
    35941 atcggtattg gacttgcatt tggtatcgtt gggaaagctg ccgggtttca tccgctggtc
    36001 gtcacgttga tgtccctgct gatctatgct ggttctgccc aatttatcac ggtcagcatg
    36061 cttgctagcc atagtccact gctctccatc gttctctcga cctttctagt caattcccga
    36121 atgattctga tgagtatgac gattgctcct tatttcaaga aaaatcggct gcttcaaaat
    36181 ctgctgattg gcacattgct gacagatgaa agtttcgcac tgggaatgaa taaattgaac
    36241 tatactggac aaaagttaaa ctttcgttgg atgaatacag ctaattggat ttcttatttg
    36301 acgtgggtgg gttcttctct tgttggcgca cttttaggga attttattac tgatccgaaa
    36361 aagtttggtt tggatttcgc catcgtcgct atgtttatcg ggttacttta tcttcaagtc
    36421 atttctgata gaaacacctc taaacgatta cagttgatcc tgattggtct cactttgatt
    36481 ctggtatatg tcggactgat ttttatccca agtaacttag tgattttagt tgtaacactg
    36541 attgggtgcg gattgggggt gtggatcaaa catgccttct tttgagttta cattgctgac
    36601 tattttaggt tgtactttag caacttggct ttctaggatc ctaccatttg tcttactgaa
    36661 aaaattcgat ttgccgcaac cgttgcttga gtatttaagt tttgtcccga tcgtcattat
    36721 gtctgcttta tggttcagca gtctatttac ccaaaatatc ggacatctgc cgcagatcaa
    36781 tctcgaaaat gctttggctt cagttcctac acttttagct gctattcttt caaaaagctt
    36841 attggtgatc gtcttggtag gaattctttc cctttcgttg attcgcttat tataaataaa
    36901 aaaacaagag cttatggcaa attactgtca tgcgctcttg tttttatcaa agtcaaataa
    36961 gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac cactaacaac gacttttcct
    37021 aatatatgtc ctgtttctac gtctttggtt gcttgtttca agttcttcgc attcatccca
    37081 tctgaataga ccttgctcaa tgtactgcgg ataatccctt catttgctaa agcagcaatg
    37141 tgcgctaaag cttctccttg cgtttcgatt tcatagttat agtctgtttt agcgaacata
    37201 tactcccagt cgaagctgat agattggttt ttccaaacac ctatatctaa cggctctttg
    37261 atccctacga tcgttcccac atgtccaaat ggtcggacaa ggtgtttgat ttgatcaaag
    37321 tagcgggtaa tatcgaataa tacagcgaca tactctactt gatcgattcc taattcacga
    37381 aaacttttac ttaagtcgtg atgataatcg attggatagt ctacacctgt ttttttcagc
    37441 cattcaaaat tcttcggact tgcagtcgcg tagacttcta ggccagccca atgcgctagt
    37501 tgattcaaaa tcgaaccgac gcccccagct ccgttgatca ctaagatttt tttcccgcgg
    37561 ttggcatttt cttctggagt cagaccaaat ttttcaaata acaattcata agcggtcaat
    37621 gaggtcaacg gcaaggctgc ggcttcttca tctgaaagat tttttggtgc aagagcgaca
    37681 atacgttcgt caaccagttg gtattcttga ttgcttcctg ctcttgtcgt tgtacctgcg
    37741 taaaaaacgc gatcgccgac agaaaatttt tgcacttcat cacctacagc aacaactttc
    37801 ccgactccat caaaccctaa gatacgtaaa gcatttctta tcccgttatc ttggcgaagt
    37861 ttcgtatcga caggattaac agatactgcg ttgactttta ccagaagatc ccgtgaactg
    37921 ggtacaggta tctgttcttt ttcatcaata aagctttttg gatcatcgat cgacaaccct
    37981 tcataaaaac cgactacctt cattaattcc ttttctgaca tacgaattcc tccttagctt
    38041 catcaagttg gagcatccat acagttttca tttcaatata tatagtatgc tatttgttcc
    38101 acttttattc atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta
    38161 aaaaagaggt tgcgacaaga cttttgtcac aacctctttt aaacggtgtt caatcaactg
    38221 accctcggta ttcgctcaaa aaacgacaaa atatgaggag ctatttttgt ttggctgttc
    38281 ttattacttg atgcaggaca gctttttcac gacctctaat tcacggtgtt caagcaagaa
    38341 atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtgtctaag atctctcgta
    38401 cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct cctggaaatc
    38461 ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg atgatttctg
    38521 aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct ttttctgtat
    38581 gttccctcgg ttctttttca aatgcataag gattcttgaa aatcccccgg ccaatcatga
    38641 tgccatctac gccatatttt tcggccaatt cttctcctgt ctggcgatct gggatatctc
    38701 cgttgatcgt gatcagtgtt tgcggagcga tttggtcacg taaagcaatg attttaggga
    38761 tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa tggacagaaa
    38821 gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca gacatttccg
    38881 tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagcc gcttcaatca
    38941 attgtgccgc tacttctggg cgaaggatca agccactgcc ttttccacgt tctgctacat
    39001 tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct gccatagcaa
    39061 tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc atcggctgtt
    39121 catcttctgt aaaggtcaat cttcctcgta cactgtcttt tccatctgga tgacagaaac
    39181 tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc tttacgacat
    39241 ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa ggctttggca
    39301 gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca agcagtgaag
    39361 tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga agcaaagtga
    39421 acagcttaaa agatgtttct gattgagcaa aaaaagactg tgacaaaaat cttatcacaa
    39481 ttccttttcc ggaataaatg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    39541 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
    39601 ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaca
    39661 aacgacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag gtcaaaacgc
    39721 tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc aaataccaag
    39781 taacggcgta caaaacaacg atgaatagca caagtacgat catcgatgtt gctactggca
    39841 catcgattgt tccaaggaat ccgtaacgga aaccggagat catataaacg atcggattga
    39901 ctttcgaaac agcttgccaa aatggcggca acatagagat ggcatagaaa acgccaccta
    39961 gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcgtca taagactgag
    40021 caaaaatccc gttgagcagt cctgctaacg aaaacaagat tgccgtcatc aataatgtaa
    40081 tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta
    40141 cgagagagcc gacgagaata ctgcgaccca ctccgccaat cacaaagccc caaatgatca
    40201 catgcgtagg aacaggcgct accaagattt cttcgatgtt tttctggaat ttttgagaga
    40261 agaaggacga agaaacgttg gcatacgaac tagtgatagc agacatcata atcaaaccag
    40321 gcacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgacca ccgatcattt
    40381 ttccgaaaat aacaaagtac aaggatgtcg tgatcacagg cggaaccagt gtttggaccc
    40441 agatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca gtaaaataaa
    40501 gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa aaacagttct
    40561 tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct tgtcagctga
    40621 tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt ctgttcatct
    40681 tcaaatgtac aagcgtatcc ttcaatcaca ggtttggtct catatggcgc taaatcaaag
    40741 ataaatgttt caaattgcag tttggataga agctctttca tactagtatt ctcgatcagt
    40801 tcacctgatt ggatgatccc gatgttgcga caaagcattt cagcttcttc taaataatgc
    40861 gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc ggagaaactc ccacatctcg
    40921 cgtctcaatt caatatcgac tcctgctgtt ggttcatcca agatcagcaa acgtggttca
    40981 tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag catacgtgca
    41041 cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct gcgtttcatc
    41101 gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg ctggaccgtt
    41161 tcaaagggat tgaaattgaa ctcttgcggg actaatccaa tctgttgctt tgctcggact
    41221 agatccgtat ccagatcata accgaagaca ttcacttttc cagatgtttt attgactaaa
    41281 gaagtaatga ttccgatcgt tgtagacttc ccagctccat ttggtcctaa tagcgcatag
    41341 aaatctcctt ctttcacgga aagatcgatt cctcgcaatg cttcgacccc acttccataa
    41401 acctttttca aattatttat ttcaagcgca tttgccattt atttcctatc tccttataga
    41461 catgattctt tattgtatca tggaatacag cagatgaaaa aacaacttgg ttccagcgaa
    41521 aaatcaggtt gctgggctca ggaaaatctc ctaatgaaag acatcaatcg gacgaagtca
    41581 gacaaccttt tcagaaaaaa caaatcgaag ataaaggatt actattcata tgtgcaacaa
    41641 aaggagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    41701 gcggaagcaa aagccaagat ttttgatcca ataaatactt tcgatcactt cgcctatccc
    41761 aatacatgcc actgccgctt ttcatcaaat aacgcggctt ccgtttttgc ggtttctact
    41821 aaagtctctg ggtaatccaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt
    41881 ccttctttta atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag
    41941 tggacattct cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca
    42001 aatgctaaag atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt
    42061 tcgatcgtat ttgtcccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa
    42121 gccacttggt ccaacaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct
    42181 tcaaataaat catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac
    42241 tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg
    42301 acatacgtag attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg
    42361 accggattca ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg
    42421 ccctcagtaa actctggtcg gcaagtgatg ggcatataag tacggtaatt caatattgca
    42481 gcggctactt ctaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt
    42541 tctaatttag tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca
    42601 tattcaaaaa tcaactctgc ttcactccca ctcttcatac gaaatgaaga acctaattta
    42661 gggatgtctc ttaatggttt agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt
    42721 tttgctagct gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct
    42781 gtttctagtg tttgtttctt cactagataa tagaccgtat tgaatactaa cccaccgatt
    42841 gccaaataga ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc
    42901 aagccaagaa aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga
    42961 tattgttttg caaaattatg atcttttttt cctaaacgag cgaattgata ttggattttt
    43021 tctctcagtt gaggattttc ctgataaaaa gcaatcagtt tttctaactg ctgatcttca
    43081 ccaaaatcaa agctgcgtaa acgttgatac aacgcttctg aacctacact agaataggta
    43141 ctgttgattc cttcgaatac ttcgaacata tccaggtcat accaagtaag atcatcgatt
    43201 tcgctatccc atgaacggaa cttcttctcc gtgagccatg cttccttcaa gctttcttct
    43261 ttatcaaatc tcggttgata aggaattttt tcccatttgc tacgaactgt ttcctttaat
    43321 ttcaaacgtg aaaaaatgtc taaagcaact aataaaataa cagctgcaat aataattaaa
    43381 acgataaatt ctctttgacc catatccctc tccgttcctt tatttgttag ctttagtata
    43441 gcactgttgc gctatttgaa aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt
    43501 tttcttgtat attacatatt tatctctcat ttatcaaaat tgacaaataa tcatagggag
    43561 gaattgttgt taattcttgt ttattctcca gtgcattgac aactgtccca agggaaagga
    43621 tagcctcagg tactattttt tcttgttctg ttggattagc taagcgaaga acaaatcctt
    43681 gttcatgaaa ataagaagga taaatcgatg atacaattaa gtcttcaggt aattcaatca
    43741 atgaaaattt acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc
    43801 tgtgaataaa tacattcagt ttctgctttt gataagaaat agattgttct aaccgttttt
    43861 ctgcctgttt tgccactttg aactcattaa atcttccttc ttctacatta aatccaaaag
    43921 tgactttata ctcacctatc tcttgagcta gcggtgtttc catcatgaca tgcccttcat
    43981 ttgtcgtatc tcctgaagct cgcccaggtc gccaagcaag attaggtttt ccaagttccc
    44041 ctgttgttga aaataaggtt atgattaatt gattttctat acgttcatac tcttttaaac
    44101 catctgcata aaacgtaaaa taatgatcat tttctgccac agagacactc ttatcgaata
    44161 tttctaaatt aacaggtttc tctaccatag tttctgacca attcggagga atgacatcag
    44221 gggtattttc aataaatccg ttttgaattt gtgcaattga tttttcagat ttatctaaaa
    44281 cattaattgc taagcgcata cgatgcgagt aaacagtatt atgaattttg agtgtgcctt
    44341 caacgatttg actttctccc agactgaacg atatttcata agatatttct tccatttcgg
    44401 gtttttcaga taaacgatct tttaaatctt taggaagttg agcttttccg taaacaacta
    44461 atgtttcttg taaagaatct ttatatactt tagctgtttc taattttaga gatagttctg
    44521 tatctccttc taatggtgaa taatcatacg tatcgccatc atttgctgaa tcgatcaaat
    44581 gaacgaaatc aagatattgt ctatctccaa ctatcaaatt caattgccca tctttaaaag
    44641 aaagtttata tttattgtta cttatctcag ttttgtctat ctcgattgct gtttctaatt
    44701 tttcattatt tttctcaaat tcaacaacag tgtatcctag tgctggcaca tttacattta
    44761 tttttatgtc aagttcataa taagctggtt catcaaaata ttcgaaacct gtagcaatca
    44821 tccgataagc atttttccgt tctggaaaat aaacttcttt ttcaatgata ttatagggac
    44881 tacctttgaa gtgtattttc tttgaatcag ctactacatg cacgattttc gatccactat
    44941 aattctcagt cgttgtgttg aaaacaagga cttcattcga ttttagcttt aatgcatcag
    45001 ccatccgttt tacaataagg ttttcgattc cgtcagctat ctcattagct tctttgattc
    45061 gatggaaaat atcttccgca acattatctg aaacacagcc accaatacta tcatgagcct
    45121 ggttttgcaa aatctttttc cataaccgac gaagtaggcc tttactgata tggataccat
    45181 ttctttctgc aatcagcatg agcggctcaa ctcgttttag aattttttgt tccaatttaa
    45241 aattttctat tttcattttt gttcttactg agccaataga acgatgaaca cgagagtaaa
    45301 ccggctgacg aagttcacct ttatattcag gtaattcttt tttgtttctg ataacttcta
    45361 cataagaagg ataatctgaa ataatattgt ggaatttact gtatttattc aacttctcta
    45421 cgacttgatc aaaatttaaa atcattgact tttgatctac acccgaaggt actaaaatat
    45481 ctttgttttt tccttgcata tgaagcattt caactgcttc atccaacttt tcttttgcaa
    45541 attcaggaat atctttaatt gcgtctaagg tcatcattcc agtcatataa ccaaatggaa
    45601 aattcatggc ataaacaaat ttatctccca atcctttcca gataaaataa ggagatggaa
    45661 ccatcgtttc aaagtttaat cctcgccaac acatgaaatt atcgatttct acttgatgca
    45721 aaagtgtagg caaattcgca ttgaacccaa acgtgtcagg caaataccct accatcattg
    45781 gatctccata tttaaggatc gtatcactga ttccaatcat taagtttctt aaaattgatt
    45841 cagcatccac taataaggca tctgtttgtg cataccacgg tcccacgaat agttgtcctg
    45901 ttttcatcag tcgttggata accttaagct tttgcggatt gatctccaaa tactcatcga
    45961 cgattgaagt ttgtccatct aaacaaaagt ttactttagg gttcctttcc aattcatcta
    46021 gaacttcagt aaagacctga tcagacaata cgatggcatc ttcatttgta aaataccatt
    46081 ctctgtccca gtgcgtgtgt tgaattatat gtgcatttac catatcagat tctcctactt
    46141 tccttcttct actgaattta cttgttgttc ttttttatac ttgatggatt ttagtgcatt
    46201 gaccattaac gctgtaacca ctcccccaat aacaattgat actaaatata aaggtatatt
    46261 cgtaacaaaa ggaattgcca taaaggttga aagcattgtt tcttgagtaa caccaaaaaa
    46321 tccatttaga ccacatgcta ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg
    46381 atctttcacg gcaaacggga tcgtcccttc agtaatcccc acaaggccca taatcatagc
    46441 tgtctttgca ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa
    46501 aaacattcca aatggcggaa tagcaattcc taataacatg gctgtactag gtccaaagct
    46561 tccgcttgaa aaagaagcaa ccccaaaagc atatgcgatt ttattgatag gtcctcccat
    46621 atcaaacgcc atcattcctc ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat
    46681 gccttctagc caattagtca gtcctgttgt cattgcttga ataggcgtag ctagtacata
    46741 ccataatatg attccaacaa ctaatgtagt aacaaaagga atcagcataa ttgattttag
    46801 actaataact tgcatcggta cttttacttt tttcaatgca ttagctaaat agccacaagc
    46861 taatccagca accattcctc caataaatcc cataccaaga ttttgacaca tcatcccagt
    46921 gataaaagct ggtgctagac cgggacggtc agcaattgaa tatgcgatat aggctgaaat
    46981 catcggtacg ataaactgta atcccgtctg accaatcatt cgcaggactc cccagaatgt
    47041 atccgtctgg tcccaaacac ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc
    47101 tgctgctacg acaagcggga tcatatagga aaccccactt tggaaatgat ttagaatttc
    47161 tcttccttgt ttcttgaatt ccattagtaa ctcccccacg atatattttt tatttgttaa
    47221 ttaatgcaat tgcttcatca atcgtctctt caccatcttt tactacctct gaggtcccta
    47281 ctgttaaaat cggtttaccg tcaaagcgac ctcgttctga aacatttgta tctactgcaa
    47341 atatgacaac atctgcctcg gaaacatccg aaattgaaag tttgttttct atccccatag
    47401 ccccctgtgt ttctacttta atatctaacc ctttcttctt cgcaaacttt tctaaattag
    47461 cttttgccat ataagtatgg gcaatccctg cgatacatgc tgttactgct actatttttg
    47521 tcatatgaaa ttcctccttt taaaatgata gagcttttac caattctttt ttatttctcg
    47581 aatcttttat tcttttaaca aactcgtcat ccatcaatct tccagataat tctgctaaaa
    47641 tacgtaaatg attagtacct ttatcagtgt cttttattgc taacaaaaag acgagatcaa
    47701 ctggttgatc atctaatgcg ccccattcaa cagaccgctt taacttggct acagccactg
    47761 ttgattcaat tactgctagg cttttgccat gaggaatggc gatatttttc ccaattcctg
    47821 tggtcatatg gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa
    47881 acccattagt ctctaacaat ttcgataatt tttcgactac acttttttta tcagatgcat
    47941 ttaaatctat cactatatta tttatattga ttttttcact gatcatttat aagccttctt
    48001 tcaatatata aactaatttt tcaaacgacg aactcgtttt taaatttgct aataactcat
    48061 gattcgaagc gatttcattg aatgtttcat aaattttatt caattcaggt tttacttttt
    48121 cattaagtgc aaaaaagaaa acagaatgaa caagtgtccc atcccaatca attccttctg
    48181 gattaacgta gacacagatt actggctttt ttataaaatc tacttcgata tgcggcatag
    48241 caaaatcatt catcgctgtt gaagatagtt tttctctttt gattgctccc tctagtatcc
    48301 cttctttcgc gtaattttct tttaccaatt ttttggtaat tatatttaaa acgttcactt
    48361 gattatcctt tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag
    48421 catcttctgt ttgttcattt aactgtagat actgttttac ttttctgaca tctgccccta
    48481 acatcaaagg actaacaaca ataacaggaa aatttgtatt ttcaatcgga atcgtgctta
    48541 taacaagttc atcagtgact tttctatctt gtagttcatt gatgcttagt acatcttcta
    48601 tctcaaccat atctgcaaaa ccgttattta ttctttgctc taataatttt gatgttccat
    48661 atccgctact acaaactaat atcactttcg ttttgtcagg tttgtaacga tccaaaagtg
    48721 attgaatatg gagtgttacg taagcgattt catcttcatt aaaatgtata cagaatctct
    48781 cttcgatttc ctctaataaa tctacactta tcataaatga ccttttaaaa gaatatttga
    48841 ttttgtccgt ataagggtta tagatattta ctcccaattt gagtctttta actgctgcat
    48901 tgagatgtaa tactagatta ttgatcaatt cttcatcagg ataaaattca tttaatttaa
    48961 ttcttactaa ctcttgtagc tcaacaccca tatctaaata ggaaagcttc gtttgatcta
    49021 agtcgtgatt catagcattt ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt
    49081 catctattgg cacaataatt gaaaatgctt tttcaatcat tttgattaaa ataagtgtat
    49141 tctttgagat ttcttcttca gaattattac tacgtgataa gaaattgctt gtttcaatcc
    49201 tttctatcgc aatagcaagg tggatagcta aagtttgaaa ttcataatct gtaaatttca
    49261 attgtgtctt attcatgtat cgattaagca attcggctac ttttttcaat atgtccttat
    49321 ttaagaaatt attcgcttca ccaatgaaat tgatattcaa gtctaagaga tctgttttac
    49381 tgttagatgc tgtaaagcca ccccaataat agttcacaac ttctgacatc aaccttcttt
    49441 tacttttttc atcagctttg atgcgcacgc catttcgatc tgaaactaag gacatcccat
    49501 gcttgtttag tctttttcta acatctttca ttgtgctttc taccgtcgac ctgctcacgt
    49561 ataattcgtc agcaagattt tgaatcttta gagaatcatt agtattcaat aatttagcaa
    49621 acacataagt tagacggtcc tccggagagt ctgcgatatt gattttatga ctggataata
    49681 aatttcgaag tgtttctttt tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca
    49741 tttgtacatc tggcattttt tggattattt ctttcaaaat taaaatatct ttgctaattg
    49801 ttttcgtaga tacatataat tgttctgata attctttgta agttgtgtgc tcattttcaa
    49861 tcaaaaagta tagtattttc atttgccgtt cgctaaattc gatgcccatt atcttcacct
    49921 cctgaataca gcttaagtgt acaattatta tctctatttt catacagaag aaagttcttg
    49981 attaatgtag aagtttctaa tattcttgat tatttcaaat caataaaaaa gcctgcaata
    50041 tttatatcac aggctttttt ctctattaaa tatatataag ataatacatt tttctactag
    50101 ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc
    50161 ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa
    50221 agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg
    50281 aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga
    50341 atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa
    50401 ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta
    50461 tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa
    50521 gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt
    50581 tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca
    50641 acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc
    50701 agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg
    50761 acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt
    50821 tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg
    50881 actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat
    50941 ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc
    51001 aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg
    51061 tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc
    51121 aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg
    51181 atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc
    51241 attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg
    51301 cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa
    51361 aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga
    51421 aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg
    51481 tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat
    51541 caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag
    51601 aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc
    51661 gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa
    51721 cattcctaga gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc
    51781 ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca
    51841 ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc
    51901 catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa
    51961 agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag
    52021 aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta
    52081 agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac
    52141 gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat
    52201 cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag
    52261 aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc
    52321 cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata
    52381 aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac
    52441 ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga
    52501 aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta
    52561 ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc
    52621 tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg
    52681 tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata
    52741 attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt
    52801 catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga
    52861 tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa
    52921 tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta
    52981 aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt
    53041 cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg
    53101 cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg
    53161 cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca
    53221 taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca
    53281 ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg
    53341 ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt
    53401 attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa
    53461 gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt
    53521 atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc
    53581 gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat
    53641 tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa aattagactg
    53701 tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca
    53761 agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc
    53821 gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa
    53881 aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct accaaataag
    53941 aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca
    54001 actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct
    54061 tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt
    54121 ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga
    54181 atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg
    54241 ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg
    54301 taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg
    54361 gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa accttgattt
    54421 ataaaggttt tcccgtaaat atattttatt atatttgatt aaatttgaaa tgtttgtgtt
    54481 ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttagattaaa tttttaggct
    54541 cattcattct caaatccatt ct
//
LOCUS       DAYYAJ010000004        50819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000004 DAYYAJ010000000
VERSION     DAYYAJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.004"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..484
                     /locus_tag="ACHATZ_000198"
     CDS             113..484
                     /locus_tag="ACHATZ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8402760.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(758..1450)
                     /locus_tag="ACHATZ_000199"
     CDS             complement(758..1450)
                     /locus_tag="ACHATZ_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8402761.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1453..2238)
                     /locus_tag="ACHATZ_000200"
     CDS             complement(1453..2238)
                     /locus_tag="ACHATZ_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8402762.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2475..3875
                     /gene="lacG"
                     /locus_tag="ACHATZ_000201"
     CDS             2475..3875
                     /gene="lacG"
                     /locus_tag="ACHATZ_000201"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8402763.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3900..4217
                     /locus_tag="ACHATZ_000202"
     CDS             3900..4217
                     /locus_tag="ACHATZ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8402764.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4230..5903
                     /locus_tag="ACHATZ_000203"
     CDS             4230..5903
                     /locus_tag="ACHATZ_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8402765.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5917..7548
                     /locus_tag="ACHATZ_000204"
     CDS             5917..7548
                     /locus_tag="ACHATZ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8402766.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8039..9604)
                     /gene="guaA"
                     /locus_tag="ACHATZ_000205"
     CDS             complement(8039..9604)
                     /gene="guaA"
                     /locus_tag="ACHATZ_000205"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8402767.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9663..10586)
                     /gene="coaA"
                     /locus_tag="ACHATZ_000206"
     CDS             complement(9663..10586)
                     /gene="coaA"
                     /locus_tag="ACHATZ_000206"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8402768.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10782..11387
                     /locus_tag="ACHATZ_000207"
     CDS             10782..11387
                     /locus_tag="ACHATZ_000207"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8402769.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11695..12711
                     /locus_tag="ACHATZ_000208"
     CDS             11695..12711
                     /locus_tag="ACHATZ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8402770.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12852..14153
                     /locus_tag="ACHATZ_000209"
     CDS             12852..14153
                     /locus_tag="ACHATZ_000209"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8402771.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14199..14861
                     /gene="deoC"
                     /locus_tag="ACHATZ_000210"
     CDS             14199..14861
                     /gene="deoC"
                     /locus_tag="ACHATZ_000210"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8402772.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14882..15280
                     /locus_tag="ACHATZ_000211"
     CDS             14882..15280
                     /locus_tag="ACHATZ_000211"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8402773.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15346..16428
                     /locus_tag="ACHATZ_000212"
     CDS             15346..16428
                     /locus_tag="ACHATZ_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8402774.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16640..18202
                     /locus_tag="ACHATZ_000213"
     CDS             16640..18202
                     /locus_tag="ACHATZ_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402775.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18195..19328
                     /locus_tag="ACHATZ_000214"
     CDS             18195..19328
                     /locus_tag="ACHATZ_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402776.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19328..20284
                     /locus_tag="ACHATZ_000215"
     CDS             19328..20284
                     /locus_tag="ACHATZ_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402777.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20383..21549
                     /gene="deoB"
                     /locus_tag="ACHATZ_000216"
     CDS             20383..21549
                     /gene="deoB"
                     /locus_tag="ACHATZ_000216"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8402778.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21573..22391
                     /locus_tag="ACHATZ_000217"
     CDS             21573..22391
                     /locus_tag="ACHATZ_000217"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8402779.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22408..23115
                     /gene="deoD"
                     /locus_tag="ACHATZ_000218"
     CDS             22408..23115
                     /gene="deoD"
                     /locus_tag="ACHATZ_000218"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8402780.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23413..24099)
                     /gene="gpmA"
                     /locus_tag="ACHATZ_000219"
     CDS             complement(23413..24099)
                     /gene="gpmA"
                     /locus_tag="ACHATZ_000219"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8402781.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24271..24852)
                     /locus_tag="ACHATZ_000220"
     CDS             complement(24271..24852)
                     /locus_tag="ACHATZ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8402782.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(24985..25668)
                     /gene="rpiA"
                     /locus_tag="ACHATZ_000221"
     CDS             complement(24985..25668)
                     /gene="rpiA"
                     /locus_tag="ACHATZ_000221"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8402783.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            25987..26400
                     /gene="rpsL"
                     /locus_tag="ACHATZ_000222"
     CDS             25987..26400
                     /gene="rpsL"
                     /locus_tag="ACHATZ_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8402784.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26484..26954
                     /gene="rpsG"
                     /locus_tag="ACHATZ_000223"
     CDS             26484..26954
                     /gene="rpsG"
                     /locus_tag="ACHATZ_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8402785.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27048..29135
                     /gene="fusA"
                     /locus_tag="ACHATZ_000224"
     CDS             27048..29135
                     /gene="fusA"
                     /locus_tag="ACHATZ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8402786.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29307..30494
                     /gene="tuf"
                     /locus_tag="ACHATZ_000225"
     CDS             29307..30494
                     /gene="tuf"
                     /locus_tag="ACHATZ_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8402787.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30901..31209
                     /gene="rpsJ"
                     /locus_tag="ACHATZ_000226"
     CDS             30901..31209
                     /gene="rpsJ"
                     /locus_tag="ACHATZ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8402788.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31240..31869
                     /gene="rplC"
                     /locus_tag="ACHATZ_000227"
     CDS             31240..31869
                     /gene="rplC"
                     /locus_tag="ACHATZ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8402789.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31897..32520
                     /gene="rplD"
                     /locus_tag="ACHATZ_000228"
     CDS             31897..32520
                     /gene="rplD"
                     /locus_tag="ACHATZ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8402790.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32520..32810
                     /gene="rplW"
                     /locus_tag="ACHATZ_000229"
     CDS             32520..32810
                     /gene="rplW"
                     /locus_tag="ACHATZ_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8402791.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32849..33682
                     /gene="rplB"
                     /locus_tag="ACHATZ_000230"
     CDS             32849..33682
                     /gene="rplB"
                     /locus_tag="ACHATZ_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8402792.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33725..34003
                     /gene="rpsS"
                     /locus_tag="ACHATZ_000231"
     CDS             33725..34003
                     /gene="rpsS"
                     /locus_tag="ACHATZ_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8402793.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34026..34373
                     /gene="rplV"
                     /locus_tag="ACHATZ_000232"
     CDS             34026..34373
                     /gene="rplV"
                     /locus_tag="ACHATZ_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8402794.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34387..35043
                     /gene="rpsC"
                     /locus_tag="ACHATZ_000233"
     CDS             34387..35043
                     /gene="rpsC"
                     /locus_tag="ACHATZ_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8402795.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35046..35480
                     /gene="rplP"
                     /locus_tag="ACHATZ_000234"
     CDS             35046..35480
                     /gene="rplP"
                     /locus_tag="ACHATZ_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8402796.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35470..35658
                     /gene="rpmC"
                     /locus_tag="ACHATZ_000235"
     CDS             35470..35658
                     /gene="rpmC"
                     /locus_tag="ACHATZ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8402797.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35683..35949
                     /gene="rpsQ"
                     /locus_tag="ACHATZ_000236"
     CDS             35683..35949
                     /gene="rpsQ"
                     /locus_tag="ACHATZ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8402798.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36007..36375
                     /gene="rplN"
                     /locus_tag="ACHATZ_000237"
     CDS             36007..36375
                     /gene="rplN"
                     /locus_tag="ACHATZ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8402799.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36412..36720
                     /gene="rplX"
                     /locus_tag="ACHATZ_000238"
     CDS             36412..36720
                     /gene="rplX"
                     /locus_tag="ACHATZ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8402800.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36748..37287
                     /gene="rplE"
                     /locus_tag="ACHATZ_000239"
     CDS             36748..37287
                     /gene="rplE"
                     /locus_tag="ACHATZ_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8402801.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37306..37491
                     /locus_tag="ACHATZ_000240"
     CDS             37306..37491
                     /locus_tag="ACHATZ_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8402802.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37527..37925
                     /gene="rpsH"
                     /locus_tag="ACHATZ_000241"
     CDS             37527..37925
                     /gene="rpsH"
                     /locus_tag="ACHATZ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8402803.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37957..38493
                     /gene="rplF"
                     /locus_tag="ACHATZ_000242"
     CDS             37957..38493
                     /gene="rplF"
                     /locus_tag="ACHATZ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8402804.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38654..39010
                     /gene="rplR"
                     /locus_tag="ACHATZ_000243"
     CDS             38654..39010
                     /gene="rplR"
                     /locus_tag="ACHATZ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8402805.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39031..39531
                     /gene="rpsE"
                     /locus_tag="ACHATZ_000244"
     CDS             39031..39531
                     /gene="rpsE"
                     /locus_tag="ACHATZ_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8402806.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39546..39725
                     /gene="rpmD"
                     /locus_tag="ACHATZ_000245"
     CDS             39546..39725
                     /gene="rpmD"
                     /locus_tag="ACHATZ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8402807.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39769..40209
                     /gene="rplO"
                     /locus_tag="ACHATZ_000246"
     CDS             39769..40209
                     /gene="rplO"
                     /locus_tag="ACHATZ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8402808.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40209..41504
                     /gene="secY"
                     /locus_tag="ACHATZ_000247"
     CDS             40209..41504
                     /gene="secY"
                     /locus_tag="ACHATZ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8402809.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41566..42213
                     /locus_tag="ACHATZ_000248"
     CDS             41566..42213
                     /locus_tag="ACHATZ_000248"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8402810.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42404..42622
                     /gene="infA"
                     /locus_tag="ACHATZ_000249"
     CDS             42404..42622
                     /gene="infA"
                     /locus_tag="ACHATZ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8402811.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42655..42771
                     /gene="rpmJ"
                     /locus_tag="ACHATZ_000250"
     CDS             42655..42771
                     /gene="rpmJ"
                     /locus_tag="ACHATZ_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8402812.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42789..43154
                     /gene="rpsM"
                     /locus_tag="ACHATZ_000251"
     CDS             42789..43154
                     /gene="rpsM"
                     /locus_tag="ACHATZ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8402813.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43182..43571
                     /gene="rpsK"
                     /locus_tag="ACHATZ_000252"
     CDS             43182..43571
                     /gene="rpsK"
                     /locus_tag="ACHATZ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8402814.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43651..44589
                     /locus_tag="ACHATZ_000253"
     CDS             43651..44589
                     /locus_tag="ACHATZ_000253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8402815.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44617..44997
                     /gene="rplQ"
                     /locus_tag="ACHATZ_000254"
     CDS             44617..44997
                     /gene="rplQ"
                     /locus_tag="ACHATZ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8402816.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45269..46108
                     /locus_tag="ACHATZ_000255"
     CDS             45269..46108
                     /locus_tag="ACHATZ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8402817.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46084..46953
                     /locus_tag="ACHATZ_000256"
     CDS             46084..46953
                     /locus_tag="ACHATZ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8402818.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46943..47740
                     /locus_tag="ACHATZ_000257"
     CDS             46943..47740
                     /locus_tag="ACHATZ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8402819.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47943..48689
                     /gene="truA"
                     /locus_tag="ACHATZ_000258"
     CDS             47943..48689
                     /gene="truA"
                     /locus_tag="ACHATZ_000258"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8402820.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49353..49547
                     /locus_tag="ACHATZ_000259"
                     /pseudo
     CDS             49353..49547
                     /locus_tag="ACHATZ_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49735..50511)
                     /locus_tag="ACHATZ_000260"
     CDS             complement(49735..50511)
                     /locus_tag="ACHATZ_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8402821.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50634..>50819
                     /locus_tag="ACHATZ_000261"
     CDS             50634..>50819
                     /locus_tag="ACHATZ_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8402822.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGF"
BASE COUNT        16116 a         9255 c        11041 g        14407 t
ORIGIN      
        1 aatagattta ataagtactg ataatgaaat ttctcctggt tgggaaaacg aagttggtta
       61 ccctgtagaa gaattttttc aagattactt tgcggagtat gccgatagga taatgagaga
      121 ttacgtaaca gataaaactc aagaagtcat caatcaatcc cagtcattaa atgaattatc
      181 tgataaggcg aaagaagaag cagttgccat tatttcttca gggattagtg actatattga
      241 tatgagtggt gaaatcaatt atggcgataa agacaaaatt gtacgcatga taaaagcttt
      301 tatcaaaact tttgatgtga aggcagatct taagtttaat gatacttacc tagtaggaaa
      361 attaattaat atccttggga cagagaaaga tacggaaacg tttatttcct ctttgtctat
      421 gttgtttaca gataagcatt tttcaactca gcttggtgga gaggaactag tggaaatctt
      481 ttaaccatta cgcccagcat taataaaagt atatcaaaaa agtactcctg atgattttta
      541 agattggttt gagaaataat gtgaaaacac tatttttaat gttaattata gatagagatg
      601 aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata cactgctata tttcactaag
      661 gatatagcag tgtatctttc atgtcgtttc actggcagtt ttttataaaa ggaggtgtgc
      721 atttcagttg cttttctttg gtagttagtt gaatcgttta agaattaacg gatgtggcag
      781 acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt tccatcttta tttagctgtg
      841 cctgttcaaa tactgttttg tttttaccga atggatcaat tgcaataatt gttccgatcc
      901 ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc gataacaaga caatcagccg
      961 gagctaagct cagtttgtct gccgctttaa gaaaaatatc cggttgcggt ttcccaggga
     1021 aagagccgtc gtcatagaca atcttgtccc agtcaaacca gcgggctaaa tcgaaaatat
     1081 caaaataaaa agcaacattc tctttgacag tagccgtggc aatcgttaat ggaatctgtt
     1141 ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt caactgaagc tcgttttcat
     1201 tttgcaagca aagcatacga taatagcttt ctttttcttc tgacaatacg gcgatctcag
     1261 aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc cgtattcgtc cgaccatgaa
     1321 tattttttaa aatatcctcg tctgataaac catcttttgt atgatcatgt atcatatgca
     1381 gccaagcagc ttcatgcaga tgagagtcca aaaacattgt tccgttgaaa tcaaatataa
     1441 tccctttcat agttacgctc ctatagtggt attttcggtt gaatcagcaa tttgcagatc
     1501 gacatattgt gcatagtgct cttgaacctc ccgcgagatc tggcgatccg taatgagcag
     1561 atcgaaatca tgcaaattat aaaacacata aaaatcatct cgattgaatt ttttattatc
     1621 aacgagtaga tatttcttac gggaattatt tagagcgatc tgctgtgctt ctccttcttc
     1681 ggtactgtaa gtagagatcg cttcgttgtg aacgccattt gaactgataa atgcttttgt
     1741 aaactttagt cttcttaaat tgtcgttggt gatcggtccg acaaatgcgc cggtattttt
     1801 ccggaaatca ccgccaatca gtagaatttc tggtccatct gcttgagcaa gaatatcaaa
     1861 aactggataa ttatttgtaa tgatccgaat tttcttgttc agtaaatgcg tcgccaatag
     1921 ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc tcgtgaatca aggaggctgc
     1981 tgtggcagca atttcttttt tttcttgaat ctgcagagtg gctttttcag tgtgagaaag
     2041 ttcttgatct aaagtgtagg aaagactttg agcccctcca tggacacgca gcagcttgcc
     2101 cgatttttct aattcatcta aatctctgcg aacggtcata tcagaaacat ctaattgatt
     2161 gatgatttca ttaacggtaa ggattccttt tttattgacc atttctacga tagtaagcaa
     2221 tctttctttt ttcaacattg tcttcacctc taatttaagg ataatctatc atataaacgg
     2281 acaacagtca atctaattcg aacaaaagta aacatttagg gtggttttta tgtttgttac
     2341 aatagatttt attgctgttt tctggttttt atgattattt gtgtttgttt ttgttgacaa
     2401 atgttttgga tgcgtttaca atgcaatcag ttagaaagag cgaaaactga ttatagattt
     2461 gaggagagat gtacatgctt aaattaccag aagattttat ttttggcgga gccacagcag
     2521 cctatcaggt tgaaggagcc actaaggaag gtggcaaagg cgctgttgct tgggatgact
     2581 ttttagagga acaagggcga ttcagtcccg atccggcaag tgatttttat catcaatatg
     2641 caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg tctaagatta tccattgctt
     2701 ggagccgcat ttttcctgat ggagctggga aaccaaatcc agaaggaatc gctttttatc
     2761 atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc gtttgtgacg ttgcatcatt
     2821 ttgatacccc taaacgtttg tttgaccatg gagatttttt gaatcgagaa acaatagaag
     2881 catttgtttc ctatgccatt ttttgttttc atgaattcaa agaagtcaag gtgtggagta
     2941 cctttaatga aatctatcca gttgcaacca atcaatattt gttaggtgtt ttcccaccag
     3001 gaatcaaata tgattttaca aagatcgtcg cctgtcttca taatatgatg gttgctcatg
     3061 cccgtgtagt gaactatttc aaagaaaacg aacttccggg tgaaattggt gtagttcact
     3121 cattagagac gaaatatgcg gcaacagacg cgccggaaga taagcatgct gcctttttag
     3181 acgatgcatt gtcgatccgt ttcttattag atgctaccta tttaggctat tattcaactg
     3241 aaaccttgac tgctcttgat gagatatgtg aagcaaatca ggccagctat cattttcctg
     3301 aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa tgactactta gggatcaatc
     3361 actaccaatg ccattttgtc aaagcttacg atggagaaaa tgcaatccat cataacggta
     3421 cgggtgaaaa agggacctct gtttataaag tgaagggaat cggagaacgt atttataaag
     3481 aaggaattcc tcgaactgat tgggactggc tgatttatcc agaaggtcta tatgacttgt
     3541 tgctgcgaat taaaagcgat tacccacatt acaacaaaat ctatatcact gagaacggga
     3601 tggggtataa agatcagttt gaagatggca ttattatgga tcagccgaga atcgactatt
     3661 tgagagtcta tttggaatcc ttaagcaagg cgataacagc cggggtaaat gtgaaggggt
     3721 attttttgtg gtcgcttatg gatcttttct catggacaaa tgggtataac aaaaggtatg
     3781 gcttgtttta tgttgatttt gaaacccaga agcgttaccc aaaagaatca gcttactggt
     3841 acaagttggt cagtgaaacc aaaacgatta tttaataagt gataatggag gaagaaaaga
     3901 tgaataaaga ggaattacaa atgcttggtt ttgagatcgt tgcatattca ggcgacgctc
     3961 gcagtacatt attgaaatta ttgaaagaag taagacaagg aaactttgaa catgttgatt
     4021 cggcgttgaa agaagccgac gaaaatttaa cccttgcaca taattcgcaa acaaaaattt
     4081 tagcggaaga agcatctggg aaagagatgg agatgggatt catttttatc catgggcagg
     4141 atcatttaat gaccacttta ttgctgagag atctgattca agactttatt gtactgtaca
     4201 gacagaatca aggataggag aagaacgcta tggatggaat cattaaacaa atcgaaaaag
     4261 ggaaaccatt ttttgaaaaa atctcacgta atagttattt aggagccgtt cgtgacggat
     4321 ttttaacagc gatgccagct attttatttt caagcatttt cattttgatt gctgcggttc
     4381 ctgagatttt tggttaccaa tggcctgaat ctgtctccac atggctatgg aaagtctata
     4441 attattcgat gggtgttgta gggttactcg tttcagcaac cactgctcgc tgcttggctg
     4501 attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa caccacctct gtgatgctgg
     4561 cctctgtggt aagtttcctg ttgttaagcg tctctccgat tgaaggtgga ttttcaacgg
     4621 aatatatggg gacaaaggga ctactggctt cctttgtcgc tgcattttta acagttaatt
     4681 tatataagtt ttgtgtgctg aaagatatta ccattaaaat gcctaaagaa gttcctggaa
     4741 cgatttcgca aaccttccga gatatttttc ctttctcttt ttcagtattt gcggccgttt
     4801 taattgattt aggcattcga caaatcttcc aaatctcatt tgcagaagca atcattacgg
     4861 tgctgcagcc gctatttaca gcagctgacg ggtacttagg aatcgctatt atctggggag
     4921 cgatggcgct gttttggttt gtcggtgttc atggaccgtc gatcgtggag ccggcaattg
     4981 cggcaattat ctatgccaat gtggaaacca atctgcaatt gttcaattcc ggtgaacaag
     5041 cttctaaagt actgacggtg ggtttaggga attttgtagg aactatggga ggaactgggg
     5101 caacattggt tgttcctttt atcttcctac tctttgctaa gtctaagcag ttgaaggcag
     5161 ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa cgaaccattg ctatttgcgg
     5221 cacccatgat tttgaaccct tattttttta ttccattttt gttaacacct gttgtaaacg
     5281 tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa cagctttatg tacgttttgc
     5341 cttgggcaac tccagcaccg atcggtttgg ttctaggaac gggcatgggt atcttagctt
     5401 ttgttctagc agcggttcta atcgtcgttg acttcctgat ctatctacca ttttgcaaag
     5461 catacgatgc tgtcttagta gagaaagaag cacaaaaagc agaggtgatc gcaaccgagg
     5521 aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc agcgagtcaa gttatcagcg
     5581 atgcaaataa agaaatcaat gtgttggtac tttgtgctgg cgcagggaca agtgggatgt
     5641 tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc accgatcagt gcaatggcgg
     5701 gtgcttatgg ttctcattac gaaatgctgc aggattttga tatggtggtc ttagcaccgc
     5761 aagtcaattc ttactatgat gatatcaaag aagacaccga tcgcttagga atcaagctgg
     5821 ttgcgacaaa aggagcagaa tatatcggat taacgagaga tcctgaaaaa gctgttcagt
     5881 ttattctgaa ccaatttcaa taaaggagga gccataatga aaatgggaat ttggttgcgt
     5941 aaagctgttt tatttgcagc tgtgttaggg gctttggggg ctgtttcaaa aactgttgaa
     6001 gcatctgcca gcgaatttat tcgcggagca gatatttcga tcctgtcaga tatggaaaaa
     6061 tctggtgcaa aatattatga agacgatatt tcaaaagatg ccttagagat tttaaaaaat
     6121 aatggtgtca actatgttcg actgcgtttg tggcaggatc cttataatag taatggagaa
     6181 agttatggag cagggacaaa tgatctggaa acgacgattg accttgcaaa acgagcaaaa
     6241 aatctgggac ttaaggtgct gctggatttt cactacagtg atttttgggt tgatcctggg
     6301 aaacagaatc ttcctaaagc atggcaaggt ctgacctttg aagaaatgaa tacggcattg
     6361 tacgactata caaagaacgt tctgtcggaa atgaagcagc tggacgttta tcctgatatg
     6421 gttcagatcg gcaatgaact gaatagcgga atgctctggc cttatggcaa gtcttgggga
     6481 gaaggcgggg gagaatttga tcgattggct gcttttttga agtctggtat ccaagctgtt
     6541 cgagacactc aacctaaaac aactccagta atgctccact tagctgatgg tggcgataca
     6601 ggagcattta cttggtggtt tgatgaaatc acgagtcgag gtgtgtcatt tgatttaatt
     6661 ggagtgtcgt attatccgta ttggcacggt tcaatgaatg aacttcaaac caatatgaat
     6721 gcgatcagcc aacggtatgg taaagaagtg atcgtagttg aaacagctta tgggcataca
     6781 acagcgaatg ccgatactat gccaaatgct tttggtgaag ccgaagcagc tgcgggggga
     6841 tatgaaccaa caccgacagg acaagcagag tatttgctgg atcttgccga tcggattcag
     6901 gcagtaccga ataatcgcgg ggcgggcttc ttctattggg aacctctttg gtacaacgga
     6961 aatgtgagtt gggcaacaca agcaggtatg aattatctca gtgttcaatc cactatggga
     7021 aacgaatggg acaaccaagc gatgtttgat tttgctggaa acgccttgcc aagcttgcgt
     7081 gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa atgcaagttt cgaaagcgat
     7141 ggaccaacga ccagtccttc caattggcaa acctggtttc aacaaggaac cgctaagggt
     7201 acgatcaaaa cggaagcggg cacttttttt ggggactaca aactaacttt ttggcaggat
     7261 caagcatacg aggcatcagc ttatcaaaat gttagtggca taagcaaagg aacttacaca
     7321 ttgagtgcct atgtaatgtc tgggggagag tttgatacta atcagttata tatcaaaaat
     7381 tatggtggag cggaactaaa tactgcggtt gttaaaacaa gtgaatggac aaaaattaca
     7441 atcaaagaca ttccaattac aacaggtgtt tgtgaaattg gggtttatag caaggcttca
     7501 ggaaatgctt ggacgaacgt tgattttgtt tcattggtga aacaatagac tgcggatcgt
     7561 ttcgattcaa gatcggaggt tcccttcagc cacatcgtta gcgtagaagg caggaatcga
     7621 acctgcacct cgcctttgcc aaaaataacc agaaacgatt tccagtcatt tttaccaaaa
     7681 cgagtaaagc accagacttc atctacactt tgtattatgc taaagagagt tcaacgattt
     7741 cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat aacataaagt ttgtttaaga
     7801 aagggtaacc ttagaattcc gtcgtgaata tgatttgagc acttagatgg atagaaaaaa
     7861 gttctcggaa aacagttcgt ttttcgagaa cttcttttta tgataagaat gaaacaaatt
     7921 aacttatatt atagtctctt ttggtaggga accatcttat ttttaatgaa aatcaagcat
     7981 tttagctatt gaagaatgtt gatttatagg gattccccct ccatattatg ttggctcttt
     8041 attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata cggttgacgt
     8101 gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat gggatacggg
     8161 caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact gtgtagtcgt
     8221 aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca gtgaaatatt
     8281 gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata gcatcagatt
     8341 cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata ccaagaccag
     8401 gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga gtccctaatt
     8461 cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat tgcatatctt
     8521 ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct gtaccagatt
     8581 cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct tcaccagcaa
     8641 gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt ttacgttttt
     8701 gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca ttgactttga
     8761 tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct tcgcctttgc
     8821 gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct ttttgtaaaa
     8881 gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact tttttgtcac
     8941 caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt gtccagttac
     9001 cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat
     9061 gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct tggatagaag
     9121 caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact ttagttacca
     9181 agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac aagacagctt
     9241 gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca ggctctacgg
     9301 aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat acaggaatac
     9361 ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca ctgtttggtc
     9421 cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca gtaatgcggt
     9481 ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc aattggttat
     9541 actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg gcaacgtttg
     9601 tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac tattttaaaa
     9661 tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt atggagaatt
     9721 atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa attcacattt
     9781 ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc aaaacgataa
     9841 taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg atccaaatac
     9901 catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa aaagtcgctg
     9961 acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac gatcaagata
    10021 tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc atgtgagtaa
    10081 acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc gatcagcatc
    10141 tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc ttttaatata
    10201 cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta
    10261 cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc tacacttccg
    10321 gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa acctttactc
    10381 aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag aggtacatag
    10441 atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt atctagttca
    10501 tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc tcttgccaca
    10561 cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc aatggaacta
    10621 ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat ctttttgtat
    10681 aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga tacataaaag
    10741 gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat cattactatt
    10801 ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta aaaggcagaa
    10861 cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat tatggttcac
    10921 gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag atcttagatg
    10981 tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag cggtttgttg
    11041 aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc
    11101 aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat gaagaaacct
    11161 atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta catgaaattt
    11221 tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg atccagaaga
    11281 aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat gcagaaatcg
    11341 tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta gctgaaaaat
    11401 agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc agttcttttt
    11461 ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa tagtaggaac
    11521 agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag aaggctgcca
    11581 agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc tctattcttt
    11641 tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa taggatgaag
    11701 ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt ctcacagatt
    11761 ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg
    11821 aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg acattcaaag
    11881 accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact gatcgaaggt
    11941 gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc tcgtcatagc
    12001 caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga cggaatcttg
    12061 ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt ttatcaaaaa
    12121 atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg gcttatcgta
    12181 aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag
    12241 atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt caacggatat
    12301 cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga acatggagag
    12361 ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa agatatcacg
    12421 gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat cgatgaattg
    12481 ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa gctgtcagaa
    12541 tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg attttatgct
    12601 gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa caaagtgttc
    12661 gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta actcggcggt
    12721 atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt ttagtaaacc
    12781 agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat gaagacaagg
    12841 aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga aacgagctgt
    12901 cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt ccagattatc
    12961 aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa gaaatcacga
    13021 acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct cttgaaggaa
    13081 taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg attttagccc
    13141 ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca
    13201 caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag ctttcggatg
    13261 aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa tcaggaaact
    13321 tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc
    13381 ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca gatgcaatcg
    13441 ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat gcaagacgtc
    13501 tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg gcggttatct
    13561 cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa
    13621 tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac gccttaggaa
    13681 gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga acgttgcttc
    13741 aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc caaaatcaag
    13801 ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc tacacaatgg
    13861 agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg
    13921 ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat cacgctgttg
    13981 gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca ctattgacga
    14041 tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa aatattgaaa
    14101 ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa taaaggaaaa
    14161 aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg
    14221 accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt atcgaagaag
    14281 caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg ctagcgaaag
    14341 aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct ctaggagcaa
    14401 acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt gcagatgaag
    14461 tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag
    14521 atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa
    14581 catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg
    14641 ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta gaggatgtcc
    14701 gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc ggtatccata
    14761 acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga acaagcgcag
    14821 gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa aggagaagct
    14881 catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag ataaagccta
    14941 tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag gcaagattta
    15001 tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg agcgcacagc
    15061 ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg tagcggggca
    15121 cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag aattctgttc
    15181 ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg
    15241 tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca ttcagtcagc
    15301 tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa aaaagcaaaa
    15361 ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc atgcggcaac
    15421 aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac tgcagcattg
    15481 atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg ggaaggacta
    15541 gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca atatttccaa
    15601 tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc tggattcaaa
    15661 acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc aacaaacaat
    15721 acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa cgttgtatct
    15781 gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc ttatacgact
    15841 gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat
    15901 gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta
    15961 ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat tgctcaaggt
    16021 atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg taacggtgta
    16081 ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt
    16141 gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa aaacttcaca
    16201 ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca aaaatcagcg
    16261 gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga cggtgttggt
    16321 ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa
    16381 atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa ccatttctat
    16441 agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga attgttcatt
    16501 tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt aatgtttgtt
    16561 tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta gatagaaatc
    16621 gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc agaatattac
    16681 caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa aggcaggaga
    16741 aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga acgtgctttc
    16801 cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag taaagatcac
    16861 aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact ttatgcttgt
    16921 tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc gcgcagggat
    16981 gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt atggattgtc
    17041 agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac gggtagaaat
    17101 tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga cagctgtatt
    17161 gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca aagaaggcaa
    17221 gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg accgctgtac
    17281 agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca
    17341 attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa
    17401 gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc gtggattaga
    17461 agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta tcgctggaat
    17521 cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa aggcagaaag
    17581 cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa aaatcactga
    17641 acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc tagatatgac
    17701 attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta aaaacggcat
    17761 gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat atgatgttcg
    17821 aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat
    17881 tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc caactcgtgg
    17941 actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa gggacaaata
    18001 caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat
    18061 tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa
    18121 tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag agttagaaaa
    18181 gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta gtgcctgtac
    18241 tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt ggttttcagc
    18301 ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga gggataggtg
    18361 aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct gtagctaact
    18421 cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg atcgtcagtg
    18481 tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg ttatgcgtga
    18541 tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt cgagcatttt
    18601 ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta tacgtaagca
    18661 catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac tcttccaata
    18721 tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt ggcggctctc
    18781 gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga
    18841 agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt gcttctgagt
    18901 atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg
    18961 cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt gttcaaacga
    19021 cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct ggatcagcaa
    19081 tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga cttggcatgc
    19141 agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg attatcttct
    19201 ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa gggaaaaaac
    19261 aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat gaagggggcg
    19321 aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac tggtttattc
    19381 aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg ggatcgtaaa
    19441 cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt tcaacttgac
    19501 gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg gtggcgtcgt
    19561 aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat
    19621 tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct tgatcaaagc
    19681 atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt tttctttccc
    19741 aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat tcttacctgc
    19801 ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg
    19861 cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt
    19921 atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta tgggcggtgc
    19981 tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg gacaagggtt
    20041 catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc
    20101 attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac cagttatttc
    20161 ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca ttgtgctggt
    20221 tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata tcaaatcaaa
    20281 ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc tcgccctttt
    20341 tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat
    20401 ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa gttcggtgat
    20461 gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt tccacattta
    20521 gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt gaaagatcat
    20581 caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat gacaggccac
    20641 tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa tggattccct
    20701 gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg taacaaacct
    20761 tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac aggcgattta
    20821 attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga aatcattcct
    20881 ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga tgatccgtat
    20941 atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt cacacgaaca
    21001 agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga ttctttgaaa
    21061 gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa cggacaagga
    21121 atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct gctgaaagtt
    21181 atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga tgcattatac
    21241 ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga cttacgaatt
    21301 ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc cgatcacgga
    21361 aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt attggtttac
    21421 agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga tatttcagca
    21481 acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta
    21541 ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag atgttacaag
    21601 aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt ttgattctag
    21661 gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg
    21721 agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta gtttatgggt
    21781 ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat gaaggtcatt
    21841 ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc cattcagtca
    21901 ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat ttgatgctga
    21961 ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat gaagatgcaa
    22021 tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa gcaacagcaa
    22081 aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg ggctattctg
    22141 gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga gccgatgcag
    22201 tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta aaagtgttag
    22261 gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc catgaagaag
    22321 ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa gaaacattga
    22381 cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa agaaggcgag
    22441 atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat tgcggagact
    22501 tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt taccggcaaa
    22561 tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc agccaccatc
    22621 tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc
    22681 ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg tgcagcgacc
    22741 agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat cagtgatttc
    22801 aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt tcatgtagga
    22861 aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt ccaattagct
    22921 gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt aggcgcgaaa
    22981 taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac cggtgaagaa
    23041 acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg attggaaaca
    23101 gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat aagaacatcc
    23161 aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca aatgtaagct
    23221 tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt cacaaggaac
    23281 aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc gagaagttgg
    23341 caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt
    23401 ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat ttaattcata
    23461 aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag agataccttc
    23521 gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa cagttttgtt
    23581 gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac
    23641 tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac gacggtcatt
    23701 tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt aagaacgacg
    23761 ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg
    23821 taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta cccaaagttg
    23881 atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa cagaagtgta
    23941 agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt ttgcttcttc
    24001 gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca atgcattcca
    24061 ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc cttttaagat
    24121 aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg gaagagattt
    24181 ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa aaaaacgaac
    24241 agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga tttctttttc
    24301 aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg aagaaataaa
    24361 ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg tttttgcatc
    24421 gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc caactaatgt
    24481 acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt ggaaatagat
    24541 ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca gcccgcgatc
    24601 tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg
    24661 gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca aatgaacagc
    24721 agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac aaccaaagtc
    24781 agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc gtgcataacc
    24841 aataattttc atggtgattc ctttctagca gtgattttct aactggatat aacagtaaaa
    24901 atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct gtcttttttt
    24961 attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa tctcaacgcc
    25021 tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat gttctactac
    25081 cccaacaagt tcatctaaat aggtagctaa gctttctggc tgttcgatga cttctagatg
    25141 cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt cgtctgtacg
    25201 caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg agccataagg
    25261 gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag aagaatcaac
    25321 gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca aagcagcgcc
    25381 gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta ttgtcaaatc
    25441 gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg cttgttcttt
    25501 tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca tacggcgacc
    25561 aatctcttct accatataat aggcggttga accggtcccg agacctacga tcatcccgtc
    25621 ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct ttgcgtccac
    25681 ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta cggtaaaaag
    25741 ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct cttgttgaca
    25801 tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa
    25861 cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat ttatttttta
    25921 tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa
    25981 caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa
    26041 tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca aactaacgta
    26101 aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc taaaaaaccg
    26161 aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga agttactgct
    26221 tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt acgcggtggt
    26281 cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt agatactgct
    26341 ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc taaagcttaa
    26401 ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta ctatgaacaa
    26461 tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt
    26521 taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt gtaatggttg
    26581 acggaaaacg cggtattgca gctaacatta tctataatgc ctttgacttg atcaaagaat
    26641 ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc
    26701 ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt gaagttcgcc
    26761 ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg
    26821 aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg
    26881 cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt gcgtttgctc
    26941 actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc tcagatagtg
    27001 tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg accagagaat
    27061 tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct ggtaaaacga
    27121 ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt gaaactcatg
    27181 aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt acgatcactt
    27241 ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac acaccaggac
    27301 acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt gctgtaactg
    27361 ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaacaa
    27421 cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt gctgacttct
    27481 tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca atccaattac
    27541 caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa
    27601 tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa gaataccgcg
    27661 aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact gatgaagaac
    27721 ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc
    27781 gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca tttaagaaca
    27841 aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg ttagatatcc
    27901 cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct gcagacgaca
    27961 gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta ggtcgtttaa
    28021 ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa aacgcaacaa
    28081 aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct cgttcagaaa
    28141 tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac acaactactg
    28201 gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa ttcccagaac
    28261 cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa atgggtgtgg
    28321 ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac gtggaaacag
    28381 gcgaaacaat catcgctggt atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc
    28441 gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac cgtgaaactt
    28501 tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat
    28561 acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc gaatttgaaa
    28621 atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa actggtttga
    28681 aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc aaagctaaac
    28741 tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt gttgctgctt
    28801 ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa ccaatcatgg
    28861 ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac gtaactgctc
    28921 gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga
    28981 tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct actcaaggtc
    29041 gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca gtacaagaag
    29101 aaatcattaa gaaaaacggc ggaaaagctg aataatttct tgaaaccgga tagtcctgca
    29161 aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata attatcgatg
    29221 atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat aaaaaatata
    29281 ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac
    29341 acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta acagctgcaa
    29401 tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat caaatcgatg
    29461 gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt gagtatgaaa
    29521 cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac gttaaaaaca
    29581 tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct gctgacggcc
    29641 caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt ccttacatcg
    29701 ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa ttagttgaaa
    29761 tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt cctgtagttg
    29821 ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa attcttgaat
    29881 taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac aaaccattca
    29941 tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct acaggtcgtg
    30001 ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt gctgaagaaa
    30061 cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac tacgctgaag
    30121 ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc caacgtggac
    30181 aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca gaagtatacg
    30241 tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac cgtccacaat
    30301 tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga actgaaatgg
    30361 ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc gctatcgaaa
    30421 acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag
    30481 aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg gacgtaatcg
    30541 ctcggaacga aggtcttagg atctttgttc cgagcttttt tatttattgc aggatcagga
    30601 aaaaagacag gagtattata aatagaagaa acacgaaata aaagagtcag tattttctaa
    30661 atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt gtgagtttat
    30721 atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt aatcagaatg
    30781 atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc acgaacgagc
    30841 gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg agggaacaag
    30901 atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat tttagatcaa
    30961 tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc tggtccaatt
    31021 ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa atacaaagat
    31081 tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt gaacccaaca
    31141 ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa catcgaaatc
    31201 aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg aatcttaggg
    31261 aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc agtaactgtt
    31321 gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga tggctacgaa
    31381 gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc tgcgaaaggt
    31441 catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa aaatgttgag
    31501 ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc aggagacatt
    31561 attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa acgccacgga
    31621 caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt
    31681 ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat gggtggcaac
    31741 cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg
    31801 atcaaaggta atgttcctgg agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa
    31861 gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc attattcaaa
    31921 caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg aattgaacct
    31981 aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca
    32041 cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa
    32101 ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg aggtggcgta
    32161 gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta
    32221 gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc
    32281 ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc
    32341 gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt
    32401 aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga cgttgtatca
    32461 aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt gcttgcataa
    32521 tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg cttgccatgg
    32581 atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag
    32641 cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga
    32701 aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta
    32761 cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa gcactaatag
    32821 ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa
    32881 tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa cacctgaaaa
    32941 atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc gcatcactgt
    33001 acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca aacgtaacaa
    33061 agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat
    33121 cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta aaggactaga
    33181 agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa atgctcttcc
    33241 attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac ctggtaaagg
    33301 tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag aaggcaaata
    33361 cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac
    33421 aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag ctggacgctc
    33481 tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta atgatcaccc
    33541 acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt ctccttgggg
    33601 tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca aacttattgt
    33661 tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg agaggaggtt
    33721 caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt
    33781 tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac gccgttcaac
    33841 agttttccca agttttgttg gatataccat cgcagtttat gatggacgta aacatgtacc
    33901 agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac ctacaagaac
    33961 ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg ggaggataaa
    34021 tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt cgcacttcac
    34081 ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca gatgcaattt
    34141 caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag
    34201 cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta gtaagcgaag
    34261 cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt
    34321 ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa taaggaggga
    34381 taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat ccgcgactgg
    34441 gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga tttaagaatc
    34501 cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga aatcgaacgc
    34561 gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt tattggtaaa
    34621 ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg taaaagaatc
    34681 catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt aggtgaagga
    34741 atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa
    34801 cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg tctaaacgga
    34861 gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca tactttacgt
    34921 gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact aggagttaaa
    34981 gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa aggagggaaa
    35041 taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg
    35101 gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa gctgttgatt
    35161 cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact cgttacatga
    35221 aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact gccaaagcaa
    35281 ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca ccagtaaaac
    35341 gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac
    35401 gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg
    35461 gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat gcttgatcaa
    35521 gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac aggtcaatta
    35581 gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa aactgtgttg
    35641 cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga aggaagaaat
    35701 caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt
    35761 gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta ttctaagaaa
    35821 tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag aattatggaa
    35881 actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt
    35941 attatctaat aacacgagat tttcctatat agattgaatg caaaatctga aaggaggata
    36001 cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa
    36061 attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg
    36121 atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt
    36181 aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc atatattaaa
    36241 tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg aacacgtatc
    36301 tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt ttcactagca
    36361 ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt tatgtttgtt
    36421 aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga aggcgttgta
    36481 ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa
    36541 caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga agcgccaatt
    36601 catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt tggctacaaa
    36661 gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa
    36721 aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa
    36781 gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac acctaaagtt
    36841 gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa aaacttagat
    36901 aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac aaaagctaaa
    36961 aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg
    37021 cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact accacgtgta
    37081 cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta tactttaggt
    37141 attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa agtacgtggt
    37201 atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga gttgttgaca
    37261 caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc taaaaaatca
    37321 atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa
    37381 cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat ttgcttccgc
    37441 gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta aaaagtaaat
    37501 tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca gattttctaa
    37561 ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct gcatcaaaaa
    37621 ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat gttgaataca
    37681 tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac
    37741 gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg
    37801 acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact tctgaaggtg
    37861 ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat
    37921 ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc
    37981 gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac agttaaagga
    38041 ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat cgaaggaaac
    38101 gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca tggaacaact
    38161 cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa
    38221 ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt gaacgttggt
    38281 tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt accagctaac
    38341 acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt
    38401 cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt tggcgaatat
    38461 gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc atagaagcca
    38521 gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt tttacttgtg
    38581 ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca ataaaaataa
    38641 agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa agagacatcg
    38701 tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg tttttcgttc
    38761 taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc
    38821 ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg
    38881 taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg
    38941 tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact
    39001 agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa acatttggaa
    39061 ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt
    39121 ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca
    39181 ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac
    39241 ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg tgcttttagt
    39301 ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc tggtggacca
    39361 gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc tttaggctca
    39421 aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca
    39481 gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata aggaggacaa
    39541 caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt cctcaaaacc
    39601 aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg gttaagcctt
    39661 ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac gttgaagaaa
    39721 tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat gaaacttcat
    39781 gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca
    39841 tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc
    39901 ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt accaaaacga
    39961 ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt attgaaccgc
    40021 tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat cgtgaaaaac
    40081 gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt aactgtgaaa
    40141 gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg atcaatcgag
    40201 gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa
    40261 tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt actgtaccag
    40321 gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac atgttgaact
    40381 tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt tcaccataca
    40441 ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga tttgttgaat
    40501 ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga tatctgacat
    40561 tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa tacgctcaat
    40621 taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg attctaacag
    40681 ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt atcggaaatg
    40741 gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt atcagagaaa
    40801 tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct gctatttttg
    40861 tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt ttccaacaag
    40921 ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct acaagcagct
    40981 atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct agttcgttta
    41041 ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct gaaaactggt
    41101 acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg atttatacga
    41161 ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct gagaaattag
    41221 cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt aaggggactg
    41281 aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt
    41341 tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa tcaatcggat
    41401 taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg aaacagttag
    41461 aaggcttaat gctgaaacgt aaatatgttg gattcattga gtaagaaagc gtgttgaggt
    41521 ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa cctcatttta
    41581 atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga tgcttatggt
    41641 attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga aacagctctt
    41701 ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga agtaacaaat
    41761 ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt attagatggc
    41821 tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa
    41881 aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc
    41941 ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa ccctacaaaa
    42001 gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga agatgataaa
    42061 cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc aatcttggct
    42121 tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat cgatgctgta
    42181 tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt
    42241 atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc cgacttctag
    42301 agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt ttccgccatt
    42361 ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa aagaagatat
    42421 gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta aagttgaact
    42481 agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc attacatccg
    42541 tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta accgtggccg
    42601 tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat tatcatgaaa
    42661 gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc
    42721 gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata attggaggtg
    42781 ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt gtagtagttt
    42841 cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta gctgatgctg
    42901 gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat gctatccgtg
    42961 cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca
    43021 aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc
    43081 gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg
    43141 gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca aaaaaagtga
    43201 atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc cattctacat
    43261 tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg tggtcatcag
    43321 ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct caaatggcag
    43381 cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa gtaacagtta
    43441 aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca ggtttagaag
    43501 tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct ccaaaacgcc
    43561 gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt gaacaagaca
    43621 cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc aagaatcgca
    43681 aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga aagagggtat
    43741 gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc aggtgctgcc
    43801 atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa aggtgttaga
    43861 gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat gtatggccaa
    43921 gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc tggcgatatt
    43981 atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag tgtctctgaa
    44041 ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt tcaagcagat
    44101 gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat ctacacacct
    44161 gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga agattttgat
    44221 aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc aatgagtttg
    44281 tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga tgaagcaaaa
    44341 aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg
    44401 atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat
    44461 actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg taacttagga
    44521 cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg tttacgtaaa
    44581 gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa actaggacgc
    44641 acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa
    44701 cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc
    44761 actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat
    44821 gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa
    44881 ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac acgcattttg
    44941 aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact tgtctaaaat
    45001 cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt tggaagatat
    45061 ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga gtaagcactc
    45121 atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt ttgaaggcat
    45181 aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat agtgttagta
    45241 ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag aaaagataaa
    45301 ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct ttacaattga
    45361 taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga cacttgccaa
    45421 aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa atcaaatatt
    45481 ggatgaagaa aatatttgga cgatccgaca gatggttgga atggtttttc aaaatccaga
    45541 caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg aaaatcaagg
    45601 gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc
    45661 tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac gtgttgccat
    45721 tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa ccagtatgct
    45781 agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag aagaaagtca
    45841 attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca atcggatact
    45901 agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt tttctgctgg
    45961 tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga agagcgcact
    46021 gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga tggtggattg
    46081 gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac accatttgaa
    46141 caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac tgcattagta
    46201 gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct tgttaagcca
    46261 acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga taataagaat
    46321 ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc acagctattc
    46381 gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt cagcgaagaa
    46441 gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga aagctatttg
    46501 gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg
    46561 cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact tgatccccag
    46621 ggtagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca gatcactatt
    46681 gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt ctatgtgttg
    46741 gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa cattgaatgg
    46801 ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga attaatggct
    46861 aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga cgctatcgtc
    46921 cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt atatgcctgg
    46981 agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt tttattttat
    47041 cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat ttttcacatt
    47101 actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca ttcgcccatt
    47161 gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac
    47221 ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg gcctctttat
    47281 tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta ctacaccgcc
    47341 tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg tacggttccc
    47401 agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta cattgatgga
    47461 cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa
    47521 cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg tcagcagctt
    47581 caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag gtggggaagg
    47641 aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta tcattgcttt
    47701 tgtattatta actgtgctac tgatcttttt gagaggataa acgttcagtg ggccgctatc
    47761 tattcttttc ttgctgatct gtttgcaaga aaggatgcga gcagtgtatg acaagaaata
    47821 ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa agaaagaacg
    47881 aaaaaatttt ataaaaaaat cattattcca gataaccttt taggaacaaa ggagagaaaa
    47941 acatggtacg ttacaaagca atcattgcat acgacggaac aaattttaat ggattccaaa
    48001 aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa aaaatggcaa
    48061 atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta catgcgattg
    48121 ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc
    48181 tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt tcagaagact
    48241 tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat atagggaaac
    48301 cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttaccctctt gatttggaaa
    48361 aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct ttttgtgcat
    48421 caggaagctc gatagaggat aaagtacgta cgatctacga ggctaagatg gaggtaaatg
    48481 aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat aagatgatac
    48541 ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa gatagtatcc
    48601 cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacat cctgaaggac
    48661 tttatttgta tgaagtgaaa tatgaataag aggttgtgac agaagtggtc agcttcaaga
    48721 aataagaagg aatttacgaa aattacttct caaaattttg caaaatttcg gcttattttc
    48781 gaaggagcta cttctggaac accgtgaatc agaggttgtg aaaaagctgc cctgcatcaa
    48841 gtaataagaa caaccaaaca aaaatagctc gtcatatttt tcgtttggtt gggcttatta
    48901 ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaatca gcgttttgac
    48961 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
    49021 ataccgaagg tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa
    49081 ctctgagtca caagtaacaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac
    49141 ttgtggacga ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa
    49201 aaaacgatgg aacgttgttt tgattgccct taatgaggac agtcggaaca ggtttgtcat
    49261 ttttatattt aaatggcaca aatttttcta attatttgtc atttagcgct gaaaattttt
    49321 gctatttatc taaaaaagtg ataactactt aagatgaagc agaatattta cgccatacgg
    49381 catacaaccg cgaaaaatat aaaaaaaaga aaacgattga acgcgttttt gcagtcgcaa
    49441 aggaaaagca tggaatgcgc tacatcaagt atcgagactt ggaaaacaat caggtgtata
    49501 cgctgattgt ttttgctgca atgaatctga aaaagttgtc cacttggcag ttggaacata
    49561 tgtcttcata tcttttatta tacaaaaata gcaaaagcac cagtctacac tcttagtaaa
    49621 gaggaaactg atccttttgt ctacattctt aaagtactta aactaagtac tttattttac
    49681 tctttaggat tgatacagta gcatctatcc ctttattaat aatcttttat ttttttaaat
    49741 aaagatgttg tctccatctt caggcactaa aactttttcg gaaattcctt tttctttatt
    49801 gaaagacttt aattcttctc ttgacagtcc ccaatggttt acagcttcca tatgaacggc
    49861 aataattgtg gaatctggta ttgctttatg caattcatat aaatcgtctt tccccataat
    49921 aagagattct ccaactaaaa aatgattatc tccagcattt gctactatga tttctggatt
    49981 atactgatca atagtttgtt taacagcttc ataccaaact gtatctcctg taaagtatag
    50041 tgtcttctca gtcgaatgtt tgaaaacaat accgcaaaca ttacctacat aattcaagat
    50101 ttctcctctg ccatgtttgc cttttgtttt aaccaattgt atagattcaa aaactgtgtc
    50161 ttctcgtagt acctccacat ttttaaaacc atatagtcga atttcattcg catcattttc
    50221 atcttgcaca aataatttta tttctttagg taggacctct ttcgctatat cgtcccaatg
    50281 atctagatgt aaatgtgtta caataacagc atcaataccg ttgataatat cttcgactgg
    50341 cattggcaaa tcaactagag gattcatttg atcttttctt ggtgatggaa aaggaccttt
    50401 cgaaataaat ggtggaagtg ttcctttttc cgctaacatt ggatcaatta aaaatttttt
    50461 acctccatag tctaagatag ctgttgcatt acgaatgtga tgtatattca taaacgttgt
    50521 ctcctttttg ttgatatgaa atatttttag tataattcaa ttccatacaa taaataaacc
    50581 gaacaaaaaa agtcttttac tatttttact taatttatga aaggttgttt accatgttag
    50641 accaaacaga tataaatatc attgaggaac tatcaaaaaa tagtcgaatc acaatgaaag
    50701 aacttggaga aaaagtacat ttgactggac ccgctgtctc ggcgagagtg acaaaactag
    50761 aggaaagtgg agttattgaa aattatacag taaaattaaa tatggaaaag ctagggttt
//
LOCUS       DAYYAJ010000005        48427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000005 DAYYAJ010000000
VERSION     DAYYAJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.005"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2113)
                     /locus_tag="ACHATZ_000262"
     CDS             complement(<1..2113)
                     /locus_tag="ACHATZ_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402823.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRC"
     gene            complement(2236..3786)
                     /locus_tag="ACHATZ_000263"
     CDS             complement(2236..3786)
                     /locus_tag="ACHATZ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8402824.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(3788..4153)
                     /locus_tag="ACHATZ_000264"
     CDS             complement(3788..4153)
                     /locus_tag="ACHATZ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8402825.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            complement(4201..5754)
                     /locus_tag="ACHATZ_000265"
     CDS             complement(4201..5754)
                     /locus_tag="ACHATZ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8402826.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(5815..5988)
                     /locus_tag="ACHATZ_000266"
     CDS             complement(5815..5988)
                     /locus_tag="ACHATZ_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8402827.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            6283..6588
                     /locus_tag="ACHATZ_000267"
     CDS             6283..6588
                     /locus_tag="ACHATZ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402828.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(6649..7686)
                     /locus_tag="ACHATZ_000268"
     CDS             complement(6649..7686)
                     /locus_tag="ACHATZ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8402829.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(7802..8710)
                     /locus_tag="ACHATZ_000269"
     CDS             complement(7802..8710)
                     /locus_tag="ACHATZ_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8402830.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(8700..9119)
                     /locus_tag="ACHATZ_000270"
     CDS             complement(8700..9119)
                     /locus_tag="ACHATZ_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8402831.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(9120..9527)
                     /locus_tag="ACHATZ_000271"
     CDS             complement(9120..9527)
                     /locus_tag="ACHATZ_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8402832.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(9514..9681)
                     /locus_tag="ACHATZ_000272"
     CDS             complement(9514..9681)
                     /locus_tag="ACHATZ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402833.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(9691..13581)
                     /locus_tag="ACHATZ_000273"
     CDS             complement(9691..13581)
                     /locus_tag="ACHATZ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8402834.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(13578..14270)
                     /locus_tag="ACHATZ_000274"
     CDS             complement(13578..14270)
                     /locus_tag="ACHATZ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8402835.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(14347..14748)
                     /locus_tag="ACHATZ_000275"
     CDS             complement(14347..14748)
                     /locus_tag="ACHATZ_000275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402836.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(14819..16879)
                     /locus_tag="ACHATZ_000276"
     CDS             complement(14819..16879)
                     /locus_tag="ACHATZ_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8402837.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            complement(17399..17539)
                     /locus_tag="ACHATZ_000277"
     CDS             complement(17399..17539)
                     /locus_tag="ACHATZ_000277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402838.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            complement(17587..17967)
                     /locus_tag="ACHATZ_000278"
     CDS             complement(17587..17967)
                     /locus_tag="ACHATZ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402839.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(17977..18567)
                     /locus_tag="ACHATZ_000279"
     CDS             complement(17977..18567)
                     /locus_tag="ACHATZ_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8402840.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(18570..18911)
                     /locus_tag="ACHATZ_000280"
     CDS             complement(18570..18911)
                     /locus_tag="ACHATZ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402841.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(18908..19339)
                     /locus_tag="ACHATZ_000281"
     CDS             complement(18908..19339)
                     /locus_tag="ACHATZ_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8402842.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(19332..19670)
                     /locus_tag="ACHATZ_000282"
     CDS             complement(19332..19670)
                     /locus_tag="ACHATZ_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8402843.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(19667..19978)
                     /locus_tag="ACHATZ_000283"
     CDS             complement(19667..19978)
                     /locus_tag="ACHATZ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8402844.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(19989..21200)
                     /locus_tag="ACHATZ_000284"
     CDS             complement(19989..21200)
                     /locus_tag="ACHATZ_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8402845.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(21204..21959)
                     /locus_tag="ACHATZ_000285"
     CDS             complement(21204..21959)
                     /locus_tag="ACHATZ_000285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8402846.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(21859..23187)
                     /locus_tag="ACHATZ_000286"
     CDS             complement(21859..23187)
                     /locus_tag="ACHATZ_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8402847.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(23253..23987)
                     /locus_tag="ACHATZ_000287"
     CDS             complement(23253..23987)
                     /locus_tag="ACHATZ_000287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402848.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(24043..25608)
                     /locus_tag="ACHATZ_000288"
     CDS             complement(24043..25608)
                     /locus_tag="ACHATZ_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8402849.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(25691..25834)
                     /locus_tag="ACHATZ_000289"
     CDS             complement(25691..25834)
                     /locus_tag="ACHATZ_000289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402850.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(25831..26007)
                     /locus_tag="ACHATZ_000290"
     CDS             complement(25831..26007)
                     /locus_tag="ACHATZ_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8402851.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(25991..26185)
                     /locus_tag="ACHATZ_000291"
     CDS             complement(25991..26185)
                     /locus_tag="ACHATZ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402852.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(26187..26564)
                     /locus_tag="ACHATZ_000292"
     CDS             complement(26187..26564)
                     /locus_tag="ACHATZ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8402853.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(26638..27873)
                     /locus_tag="ACHATZ_000293"
     CDS             complement(26638..27873)
                     /locus_tag="ACHATZ_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8402854.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(27873..28655)
                     /locus_tag="ACHATZ_000294"
     CDS             complement(27873..28655)
                     /locus_tag="ACHATZ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8402855.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(28670..29242)
                     /locus_tag="ACHATZ_000295"
     CDS             complement(28670..29242)
                     /locus_tag="ACHATZ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8402856.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(29376..29741)
                     /locus_tag="ACHATZ_000296"
     CDS             complement(29376..29741)
                     /locus_tag="ACHATZ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8402857.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(29909..30337)
                     /locus_tag="ACHATZ_000297"
     CDS             complement(29909..30337)
                     /locus_tag="ACHATZ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8402858.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(30334..31686)
                     /locus_tag="ACHATZ_000298"
     CDS             complement(30334..31686)
                     /locus_tag="ACHATZ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8402859.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(31686..31970)
                     /locus_tag="ACHATZ_000299"
     CDS             complement(31686..31970)
                     /locus_tag="ACHATZ_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8402860.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(31967..32176)
                     /locus_tag="ACHATZ_000300"
     CDS             complement(31967..32176)
                     /locus_tag="ACHATZ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402861.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(32314..34557)
                     /locus_tag="ACHATZ_000301"
     CDS             complement(32314..34557)
                     /locus_tag="ACHATZ_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8402862.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(34550..34894)
                     /locus_tag="ACHATZ_000302"
     CDS             complement(34550..34894)
                     /locus_tag="ACHATZ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8402863.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(34887..35657)
                     /locus_tag="ACHATZ_000303"
     CDS             complement(34887..35657)
                     /locus_tag="ACHATZ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8402864.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(35868..37805)
                     /locus_tag="ACHATZ_000304"
     CDS             complement(35868..37805)
                     /locus_tag="ACHATZ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM8402865.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(37866..38408)
                     /locus_tag="ACHATZ_000305"
     CDS             complement(37866..38408)
                     /locus_tag="ACHATZ_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8402866.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(38410..39552)
                     /locus_tag="ACHATZ_000306"
     CDS             complement(38410..39552)
                     /locus_tag="ACHATZ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8402867.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(39553..39867)
                     /locus_tag="ACHATZ_000307"
     CDS             complement(39553..39867)
                     /locus_tag="ACHATZ_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8402868.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(39872..40078)
                     /locus_tag="ACHATZ_000308"
     CDS             complement(39872..40078)
                     /locus_tag="ACHATZ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402869.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(40175..40792)
                     /locus_tag="ACHATZ_000309"
     CDS             complement(40175..40792)
                     /locus_tag="ACHATZ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402870.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            41187..42137
                     /locus_tag="ACHATZ_000310"
     CDS             41187..42137
                     /locus_tag="ACHATZ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8402871.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            42130..42576
                     /locus_tag="ACHATZ_000311"
     CDS             42130..42576
                     /locus_tag="ACHATZ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8402872.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            42579..44411
                     /locus_tag="ACHATZ_000312"
     CDS             42579..44411
                     /locus_tag="ACHATZ_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8402873.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            44427..44630
                     /locus_tag="ACHATZ_000313"
     CDS             44427..44630
                     /locus_tag="ACHATZ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8402874.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(44681..45817)
                     /locus_tag="ACHATZ_000314"
     CDS             complement(44681..45817)
                     /locus_tag="ACHATZ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8402875.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(45807..47762)
                     /locus_tag="ACHATZ_000315"
     CDS             complement(45807..47762)
                     /locus_tag="ACHATZ_000315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8402876.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(47755..>48427)
                     /locus_tag="ACHATZ_000316"
     CDS             complement(47755..>48427)
                     /locus_tag="ACHATZ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFM8402877.1"
                     /translation="GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRA
                     LAEKPWSFGEVRYDETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMW
                     LLGLLESRMHMVWLRSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILD
                     LREYEGGTLADLYNKDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQE
                     MTIND"
BASE COUNT        13264 a        11102 c         8812 g        15249 t
ORIGIN      
        1 cacatcgaag aataaatgac agaaaatctc tctcctcagc aaaactagag caatctattt
       61 ttttcaatgc cgtgtaatct gtatcttgaa aaagcttatc tcctaattca ttgccatact
      121 cttttccaat atacatttta ttggaaggtt gaacgtcacc agcaacgttt ggaagattaa
      181 atggaacttt agacagctca cttccagctg atagtttttc attgtagtta tcataaaacc
      241 aattaatgaa actgatagat ttttcaacag tgtcaatttg tcgattaatc gttccaatga
      301 tcaaatctga actgctttgc tcagtgaatt caattgcaag atatttttca ctgaatgcat
      361 ctagaatacg tttatctcct gtacgttcag ctttgtatgc attagctgtt tcttcccatt
      421 tatttttcca acttgatgaa gtcttaatct gataaattaa ctcatcaaca tatgtttgtt
      481 tcaaaatgct taaactaacc caagaagaca gctcttttgg aagcacagta acacctgtat
      541 cggtcggtaa gaatacctta tctgatttca tcaaccaatt gtcactattg tcctttaata
      601 gcttagggac aatcggagaa cttttatatt gctcactcca ccataagaac acttttgtcc
      661 ttattctatt ttcccaagaa gatgaaagaa tgttgatttt atcagatagt tcatctacaa
      721 caaacgacag gccagtgtat tcattataat tagcaagttc ttgtactagt tggtcatttt
      781 cgtttgtttc taataattgc aacaaggtgg aaaatgtctc acctattagc tcctcaggga
      841 attcttgaag gaaaagtttt ggattatctt tgatagaaat atattgccaa ttgacagtcg
      901 gtagtatttg ggcattagat aaaataccca agaattcgtt cattaagtca aatacatcta
      961 agtcccatag tttattctct ttaaaaggag ttaccatttt taatgctatg tcttcatatt
     1021 caggcttaga taatttttct gctacttcag aaataaattt gagcacctca ataaatattt
     1081 tcttatttat ttgttcacta tcatccggga cgtcatcacg tgaattattt gttataaacg
     1141 gtgcatttat aagtgcatga attggtgtac caaaattacg tactggaaag tagcaatata
     1201 gtttttcttt ggagtaatcg taatcagaat ctttagaaac ggctagtgca attttagcaa
     1261 ctttatctcc tatcatagat tctttgtcaa aaacaaaata ttcttcagac ggtgattgac
     1321 cagtaacatc acttttttct atcaatacat catttccatc cacaattttc tctgatattt
     1381 tttcatcatc agttgtttca ataacaattt ttgttatatt tggtaaaaat aacagtgact
     1441 tataaaatgg ctgctgaagt tgtttagaaa cactcgtgcc tttacttaaa ttagtttctt
     1501 ttagttttac tcgaattaat gtatcaaaac cagaatcaat tttgtttatt ggttgaggac
     1561 agttcattat tggaaaacat aaatcataat cttctttttt ccaatttttc ttctgctcaa
     1621 aaattaattc actgaatttg tcagaatctc ttgatacata ctgctcaaaa agcttttttg
     1681 tgaattcttc actaaatcga atagcaaatt ctcccgaata cagttcgacc cactcagcat
     1741 cgtttagcaa ggaacgaaaa ccagtcccct tatttccgat attttgtgat gatttattac
     1801 tcgctcttcc taagcacaat ctttcaattg tatcttttga aaatgaagtg cctgtattcc
     1861 caacttccaa gatattattt ttatatgtta tctgaacttt cacttcgtca ttgctagcat
     1921 cattttctga ttttatatct ccataggcat cgtctacatt ctgtaataac tctaaaatct
     1981 gacgcccatt ataatcacgc atcaattcat attcttcgct gcaggccttt tcaagttcat
     2041 tactcaactc agaatccgta tatttacgaa taaaaatatc ccttaattca tcaatggtat
     2101 tccattggct cacagaactt cgcccccagc ctatttttta cataatatat tatatcatca
     2161 atcactttat gaaatccaat caataaagac gttaatatgg ctacccacat taacgtctag
     2221 tcctatctat tcaaattaaa tttctacagt cactctcacc ccacttttga aatcaacaac
     2281 aatgttcttt tcaaaaatgg tgaccttctc aatcaacctt cgaacaagca cttcattata
     2341 ctcattgatg acatcgtgtt cttttacaaa gactaccatc tcatcaaccc gttgttgcaa
     2401 attagtattc gctgctattt gttttgcaac cgcatctcgt tcatctctta gtgaaaatat
     2461 ttggcttgcc aattcatcgt aaccctgacc gccaactgcc aggctaataa tttcagcttc
     2521 aagctctttc aactgactgt ctagttctgc caatcggtca gttaatccat cagtcataac
     2581 tgagttgata ttgtcattta attgctgaag aaaagcatca cgatcgatta acgtttcatt
     2641 gattgccttg ataacagctc catgcaggtc tgtttcgtag attgttcgac ccgtgcaatt
     2701 atgttctcgt ttatccactc tggatttgca tcgccacaca gttcctcgat tcttccaatg
     2761 aactctgcgg taagcattgc cacagtcacc gcaaactgtg ataccagata acgcatactt
     2821 cccagaaaag cactgtgact ttttgttttt cattgattta cgccggtcta gttcttgctg
     2881 ggcttgcatg aagatatctt ttgggataat ggccggatgg ctatcttcaa cataatactg
     2941 ctgagcgtaa ctcccgtcat tcttcactcg tttctttgtc aagaaatcgg tagtatatgt
     3001 tttttgaagc agcgcatccc ccatgtactt ctcgtttcga agaatcaatc tgactccttc
     3061 tcctcgccag agctttcgct tccctcccgt taggatgcca tcagccatta gaccattagc
     3121 aatttcttgc aaggaactgc cctcaaggta ttcgcggaag atgcgtttga tgacttctgc
     3181 ttcctttggt tcaatcacca agttaccatc ttcgtcctta gtgtacccaa gaaaatgatt
     3241 gtggttgata cgtagctgtc cctgctggaa acgatactgt acgcccatct tggtgttttg
     3301 acttagtgac tggctttctt gctgggccag acttgccata atggtcaaaa gtacctcacc
     3361 cttcgcatcc attgtattga tgttttcctt ttcaaaaaac actgcaatct tcagttcttt
     3421 taactgtcgt atgtattgca agcaatcaac cgtattacgg gcaaagcgtg aaattgattt
     3481 tgtgatgatg tactcaactt cgccgttttg acacgcctca atcatccggt taaaaccgtc
     3541 acgctttttc gtcccagttc ctgaaatccc ttcatccgca aatattccta caagctccca
     3601 atcgggattt ctttgaatgt agtccgtata gtgttgaacc tgtgcttcgt aagaactcgc
     3661 ctgttcctcc atttcagtag aaacacgaca ataggctgcc actttcaact ttggtttctc
     3721 ttcggtattc tctgctgccc attgtctagg cttcattgga ataaccgtca cctgttttcc
     3781 tgccatttca cttaacctcc ttgatttttg aatatgcata ttgcgcttgt ttgaatgggt
     3841 cagaatactt ttcggtaatc ttgccagatt taaaatgagt gggtattacc ttcgtatctg
     3901 gcactttgat ctccttgcgt tttttaaaga acttaattct tcgttcttgc tccagatccg
     3961 cttggaagaa aatatcctca tcaatgattt ttggatatat ctcatttcct aagtaaacct
     4021 cattcatcaa aatacgtttc ataaagggat gcggcttgtt aatgccagag cgtttgccag
     4081 cttcttgtaa gccaacaccc tcaaggtaaa tattgaagaa ctcacgtatt tgttgagctt
     4141 gctgttcatc cacgttgatt tctccattct taatgacata cccgtatgga attgtattgt
     4201 tcattctggt aacctctctt tcaaaagtag tccgcactta agttcaaatc cgacttcttg
     4261 gcggttgtaa actgtaatgc tgtttgtgaa cttcttgaat aaatcttcat cccagtccgt
     4321 caacatctct gccatccgcg cgaatttgta taaggcttct agttcaattt gctgagtgct
     4381 tttgccgttg actgactcaa tgatgaggcg ctgcttttct tgtgcgacag ccctctcttg
     4441 ttgaagtttg ctttgttctt ctaaagacac cttcgttgat aaaatactgg aagcagtcaa
     4501 acttgccaga acctcaagct tttcttgaat cttgtcgatt tcatcagcaa gcacttcgag
     4561 cccttcctga ttcgcacctg ctttattcaa agacttcata aaaggttcca gaaccaactt
     4621 gtgtccgaaa atcaacttat tcaacatggt caaataagca accttcaaat cttcatccgt
     4681 aattggtaat gcatcgcagc tatctttatt tttaagatgc cccgtacact tccactgaac
     4741 cttcttcgta ttacctttcc aataagtgtg aacccgcttg aatttgctgc cgcatttatc
     4801 acagatgatg ttgcctgaaa atgcgtatcg cttggtgtac tttttcccgt caccagtatt
     4861 ccctttggcc tcagcttttc tccgaatgag ttcttgaact cggtcgtaat cttctttaga
     4921 tactatgggt tcgtgattgt ttttgatctg gtacattggc aattcgccct tgtttatgcg
     4981 tcgtttgtac tggtcatcaa caaaggtttg ttgcatcgtc aaagtcccgg tataaacttc
     5041 atttttcaaa atacttctga ctgatgttgc ttgccaattt gccccacgtt tagtgaattg
     5101 ttttccatcg tttaaccgct tggcaatcat tccagtcgaa atgccatcta atgcccactg
     5161 gaacatttgt ttaacaactt ttgcttcagc tgcaacgatg tgaaaatcat agcctttccg
     5221 cttgtagcca tacggcgggt aagctttgaa tttcccttta gacatccggt ttcgaatcgc
     5281 ccacttcaag ttttctgaat gggaatagga ttcactttct gccaagcttg ataaaattga
     5341 aagcaacaac tcgctttcca tatcaccagt attaatgttt tccttttcaa aaaagatggt
     5401 gacaccaata tcagttagct gccgaaccag attcaagcag tcaactgtat ttcttgaaaa
     5461 tctggaaata gactttgtca gaatcaaatc aatctttcca agctctgcat ccttaatcaa
     5521 tcgttttaaa ccatcacgtt ttacagtctt ggttccagaa atcccttcat cataataaac
     5581 accgacatag tcccattgtt catggttctt gatgtgattt tcatagtgag taatctgggt
     5641 ttgtaaactt gccagctgtt cattacttga tgttgataca cgagcgtatg ctgccacctt
     5701 tagtttggaa gcttccggca actctggacg tgccaattta tgtatcgttt tcatcagctc
     5761 aactcctttc cattaggtga catgttagct ctaaagtgcc gttatatcaa cgtttcagac
     5821 cattatcctt gataaaaaag gattgaatgt ttcccggttt ttattgtcga ttttgacgta
     5881 ttcttccagg ttgattttac cggtaacaag taagttcaga aggatctgct gtgaaatgta
     5941 ataggctgct tcatctgaat agttaatttc tgttttcttg ttagtcattg ctttcgcctc
     6001 ctcaagatac aggcgaagaa aatccatgaa agtaaaccca atcacaaatt ttcctttgcc
     6061 tttcatccat atgcgaaaga aataaccgac tcgaacccac aaaacgaaaa aagcctgcaa
     6121 gaatgaaacg aatcactctt gcaggcttct aagtttgttt atatatagta ggaattattc
     6181 tttgtaaatt ttgacttttt acttcccaaa catcaaataa gcctttacaa gcttcatcca
     6241 ataaataaca atataaatat caattgtggg aaggagaaag ttatgagtag ttttgatatg
     6301 gaagggttag aaaaagaata tgaacatttt tgcaaatctg cgacaacaaa ggaactggag
     6361 gaatttgatg aaagcttcaa acaagaattt gaaaagtgct ttaaaaccat agaggctgaa
     6421 gaagctttat accagttttt acaagccaat ataaacgcga tgaataatcg tatggaatca
     6481 atagaatcgc ctcttttctg catcaattac aaagagcagg tatttcagtt aagaaatcat
     6541 ttaatcctaa tgcaaaatgc tgtaaacacc taccttaaag aaaactagac gaaaaaaagg
     6601 ccccacaata aaacttgtga ggctgttttg aattataaag acgtcaaact acttattgaa
     6661 aattttgtag ctcgttggaa ccatcgtgaa gttttcttca gtaattgggc catcataaat
     6721 tttgattttg caaacttttg acgccacatc aaggtcgtga acaaagtaaa ttttatatgg
     6781 tgtatctcca agggaggcag caacctcatt agtactcatt tcaaatccgt cagagaagtt
     6841 tgtttttgaa gctttcactt caatatggat ttcattacct gattggtcaa acgcacaaat
     6901 atcatagcca tgaccatctc cttcagtttt agaaacatgt tcaggaagtt ttgttttgtt
     6961 cttttctgct tcctgaatga ggaaatcaag tacgatttct tcgcctagag cacccgtttt
     7021 ttgttttgcc ttgttgacac gctcccaatc agtatggcgt ccagtgaatt ttcgtttctg
     7081 agtcccattc gtgcgatttg gtcccacttc atcaacaaga acagtaaatc cttttgaact
     7141 ttcttgctca atttcttcaa cttcctcgtt tgtaagatcc agttcagacg gcaacgattg
     7201 tgtatcatcg attgggataa gatgacgatt ttcaatcaat gcaacaaaat cggcataggt
     7261 aatttcattc attccatact gatcccagaa gtaggcccat gaattcggct ttttacgctt
     7321 ctttttgcta ggtgtattcc aatcgaagtt ttcaataaat gaatcacctt gtttaattgc
     7381 cggttcgatt gtaaatgcgt gtgtaattac tgccgtaaca tttccagctt catcaggttc
     7441 agaaatgctc tcaacgtatc cgcccccgta aatttcccac ttacggtctt tagtaatttt
     7501 ccccggatga cgattcaaaa caaccgcgcc ggctttcaat ttaccatatt ggcgcatgtt
     7561 ccaaccgtaa cttttagcat catcagcgta tttcaactga ccatcatctt ctgttttccc
     7621 gtgatctaaa atgaaagctt gttggctttc tgttggttct ggtaacaaat ctttaaacgt
     7681 aaccatatcg atacctcttt ctataattca atcggaacaa atttatgttc tggttgaatt
     7741 aataataaca cgttcgatat tttaagtcaa caatccggac ataattttgt tccgattaaa
     7801 tttatgaaat caaaatataa tcgtcagatt ccgtcttacc agtattctta ccgacagcac
     7861 aatatccaat cgggcaacga gcccataccc aaccatccgc aatcgtcatc ttctctgact
     7921 tcggaagcgt ccaagttgcg cctttattgt atgaaccaac aaccgcacca cttaaacttg
     7981 gcttgttgcg aacgttgaga ccactcactt gaaccacaag agttttcacg ttggatgttg
     8041 atgacccaga tggataaact tgattaccgt tctggtcgaa caccttataa ccagaatggc
     8101 tatctgcagc ccgcttcgca ttgttcaagt ccttataggc accaatttgt gacttggcgt
     8161 cagcccatga tttgcgaaca cgatacagac cactagctgg cgtgcttggg gttgatgaac
     8221 cattattaat cgcattggct ttatccgcaa tctcctgcag tttaccaagc aactgtcccg
     8281 gacatgatgt ggccgaaaaa tgtgagtgag ggaagatatt cttgttcacc accaatttcc
     8341 ctagaccata acgtttggca atatctgcac acaactttgc acttgcatca atagttgctt
     8401 gattgatacc ccagttcggt gatccagtgc tattgcagtg ctcaatcccg atacttgaga
     8461 tattggcgtt ccaattacca gcgtgccaag ctgtgtttcc ttcgtccacg tatgctcgaa
     8521 tttctccatt ttttccaata ccgtagtgtg ctgacgcctc acgcgttttc caaatatcag
     8581 gaacaatatc atagttggtg gttgccatgt gatgaatcac tatcttatct actttggcac
     8641 cactacgacc attcgagtaa tggctaaatg actgaaatcc atattttgtt gctagattac
     8701 taattcccat tttctgtttc ctccttcttt tcgtgtaact gctgcaagat ttcacgcaac
     8761 ttttccggca ctggcagtcc aatcgccacg gcattctcca gcagtgaaat tccttcgttt
     8821 gagatataga agaaaatgac cgcatcacgc agtacatttc cacctttgat gacttccaca
     8881 tcaagcatat gagccacacc aaccaaaaga aaaatcatga ttttcttagt gatgcccttt
     8941 gccccaatct tgctggatag tttcttttcg ttgattgcac gaagcagacc agttacatag
     9001 tccactacca caaaagttac aagcgtataa atcaaaccat caattcctcc cacataccat
     9061 ccaatcactc ctccaccacc tgcgatggcg agttgaatcc aattccacac ttctttcatt
     9121 tacaaatcct ccaattgttc taaaattaaa tcgagcattt cagcatcttt ccctgaaatg
     9181 gtgacttcac tattttcaaa tccagcaacc aataaaggca gcttgtcttg aagttcatcc
     9241 acgttgatgt gggtttcttc gttgagcaag tctgcctttt ctttgtgata ttcagttgcg
     9301 gtttctggga tgagtgaaaa ggcaccgtca tgttggacca aattgccgtg ctcatctttc
     9361 tttccgtatt tttgaagcaa gtctacctcg tcttgataga gttcatcaac cttcacctgg
     9421 atgagttttg caagcttcgt tcgcactcgg ctaatctttg gtggtaagtc aaaacctttt
     9481 aagaaactca tcacgtccac caaatcttca tttctaattt tgagcattat ctatcttctc
     9541 ctttaaatca ccaatctgga cacaaagatc atccacgacc atcacaagca aatcaaccat
     9601 atccgtccca actttcattg gctttggaat atctgcctcg ataggttcag ttactatcac
     9661 ttcttctttt tcttcgttca tggcggcctc ctaactgtat gtggtcattg aattaaacga
     9721 ctgcggattg taccaaccac tcgctgtacc gttgctactg aaaccggttg gtaaagcgat
     9781 tgttcgtccc gcaagtttgg cacagatttc acggatgact ccaaagtcaa cccacttccc
     9841 atcatctccg agcatcagat tagaaccgtt gaatgcaata ccagcgctgc cggaacttga
     9901 gccaatctta gtataaaggg aactactaaa tgtaatccca ccaacagata tttgatgttg
     9961 tgtccctata cgaagcattg atggtaaata aatgtcgtcg tcaaagtgga accccttctc
    10021 cataccagac tgcaatgttc tcgcatacca agaaagcttc tgggtgtaga cactcgcaga
    10081 ggaggaatct ttatgagccc atgacatata ctctgttcca tactcaagat caaataccaa
    10141 tccacgccaa tcagagtgac cactgatgtt atttgcacca attgttccga ctgtcgttcc
    10201 accacgtcga atggtcattc cctgcgagtt tatttcagtc acaagagtgc ttcctgaata
    10261 atatccaatc gtagttcctg tgattcgaat ggacgaactc gcagtcaatg ctgttaggat
    10321 attagcagca atcttatcag ccgtaatcga gttcgcgcca atacgggctg ccgacaaagt
    10381 tcctgtggta atcttcgccg catcaatgct tgcaattttt gcactttgaa ttgtggcatc
    10441 tgcaatcttt gcgtttgtga tggccgcatt ttggatcatg gcagaactaa tcactgcact
    10501 atcaatggtt gtttgaccag tgatgtgcac cttgtttcca gcaatcaaaa tactttcagg
    10561 tgagatattg atttgattaa tcacgtctcc tttaccaact cgtaaattga ttaagtcttt
    10621 ctgcatgaga attgctgaat ataatgtgtt ggcattggta tagtcttgcc ttgtggtcgt
    10681 aggataaata tttccttcat attcatcggg tttgatgctc gtgttcactc ggactcgcat
    10741 tttcaatgtg ccactcggga ctgtaattgc tccgctcgaa ccagtataag agatttgatg
    10801 ataacttgaa ccatccgacg ggtttagagt aaaaggtgtg ttgcttgaat tataaatccc
    10861 agcatatgtg ccagtcttaa ctgaaaagaa agctagatac acgctgtcag tagtaatccc
    10921 tgatatctgc tcagccgtta acgtccaaga ataagtggca ttgctgctac ttgtaacggt
    10981 cgaaatctgt tcccaagcac cacctgtata aagcaagaca atattatctg atgtatcctt
    11041 gcgaccattc cacgaaatta cactcggagc gactttgcct tccaatggca atttgtaaat
    11101 gaccagataa tctcctgaat ttgaataggg gtttgttgtc acgccacttg taatactcat
    11161 cgattcaacg ttcttactta ctagataact cgcaatggtc gcattagtat aagttcccgc
    11221 cgttagggtt tcactaaatt gttttgccgt tgggacaaaa tcaatgtaag gtaaatccat
    11281 gccgtagtaa tgatagtaga aagcagcttg agttccgtca cgcagttgcc cgatatactt
    11341 atctcccact ttgacatcaa ggaactcact tcggagataa ttggttgcgg acacttctga
    11401 accgtcagtg gtactcaaac ttccttgttc ccattgattg actttaaatg gaacgccgat
    11461 tgcttctttg gtttctgaaa cacttaacag taaggcatca gtggtcgctg taagttttac
    11521 gacgttactt atctcggata aatcaacata gccatttgtc acatccgttt tcaacatata
    11581 gcaactgatg gtttctggtg catcactagt tgttaaacga atgcgaataa aagcggcact
    11641 gcttggaaca gtaagcggtg tggtggtgtt tccaaattgg gtgtacgata aaaaggactt
    11701 atcctctttg taataaagca gataagccgt tgcgtaagta gagtaaagtt tcccacccac
    11761 aaatgtttga agtagatact tttcgccatt tctaactctt gtaaaacctg agcgaacgta
    11821 tccaccatag gtattttctt ttccatcatt tgtattcaga ccaccaactg cccactgcaa
    11881 atctgcaatg ctcataccgg ctaaaacatt gaccgcttta tttgttttag aaagagatag
    11941 ctcaaatcta tcagcatttt gggtgatggt gctttctgca gttgtaatac gccctgtcag
    12001 attatcaacc gtcgattgac ttgccttact agcaatctgc ccagcttgca ctgtcagagt
    12061 tgattcagct gtgattactc gtccttcaag agttgacata ccactcgcca ctaatgaaat
    12121 cttgctagta ttctgttcaa cggatgtagt aagcgtttca acacttgcac ctacactttc
    12181 agctgcattc aaagcattca cagcatcgtt aaatgcttga ttggcctttt cagtggcagt
    12241 attggcatcc ttttgtgctt gttccacggc ttgctttact tcttctgccg tcatatctgt
    12301 cataagtgga taccactgag tgacgccatc acggatttca tacacccaca tttcaaactt
    12361 gtcaccattt tctttgtacc aaacgtcacc gagtttcgga ttatttggag ttgtcgggcc
    12421 atagaagttg gtgtttttac catttgccga actcaatgcg taattggcat cgtctttggc
    12481 ttgctccgcc ttgttgtcca cccggttgat gtccttcgcc acatccgtga attttggcgt
    12541 gatgtttccg agtgttactg agatatacgc tttcagaaat ggatcgtatt tgtatgagac
    12601 cattctagct gtgatgttca atccatcttc ggcatgaata actgaaacag tatcacccaa
    12661 attaatcgtt tcaagctgtg caaaatcttt atattcttca gttcgctcaa gcggggcaaa
    12721 ctccacatca taggtggctt ttggcaaatc gaccttgtta gctgagtatt ctaacccagc
    12781 caatctgcga agttcatcat aggcctcggc ttgagtaaac tcatcctctt catcaccaac
    12841 tttgacgttg tcgtacttga tgactttgat tttaggctgg acgtattggt tgatgtttgg
    12901 actgtccacg tatttttcgg gaagaaatag tccatcatac ccttgtggca taatgcgagt
    12961 aacaattcca gagtaatcaa catcagcttt atatcctgtc agattcttct tgtctcggat
    13021 ttgtacgccg ttgttactgc cacgagtcgt taacattgaa atcgtgaagt tatcgcgtac
    13081 aatttcacca ccataactgc tttgaaaacc attctctagg tcactatcaa gcaacacctc
    13141 tacaggattt agtcgcacca agcggacatt attgaccgca ctgatgtttg atgtaccggt
    13201 aaaacggtgc atgaattgag ttgcaccaag cagttgtgtc aaagctcccg acccgttctt
    13261 gttgacgata taagtatctt caataaagtt tgtggctagg tcatagaaaa tatgatgagc
    13321 tactatgtgc aaaaaaccac caagtgcatc atctcgttca acaatcctga aaagctggtc
    13381 ggacagttct ggtactggtg ctttcacaat catttctggc aacaattcct cactgtgtgg
    13441 agcatgaatt ggataatcga attcaaagga aaagagtccg ttcaattcct cactcaccac
    13501 ggggttcaga atgttgccat caaggacagc gagaccgtta tgattgaaat ttgattctgt
    13561 tttgttgtaa agcgtaatca taagtatcgc cacctcggtt ctatttctac tttggttata
    13621 cctgtaccaa gagtcacagt attactgccg actcgaaact caggaaagtc acctgtcatg
    13681 tattgattgg ctgccacatc cccgtaaaag cattctttca aatccgaatc tagtgtcagg
    13741 ttgccattcg tcagatttaa cacgataggc ttgccattta tcgtgagtgt cctagttcca
    13801 ttgccgtaga ccttaatctt cggcaatgag taaatgttgc cgggattatt gaccgttcct
    13861 gacgtggttc gattgatagt cgctacattg gtcttataac gaaaaggcga acaggttaac
    13921 tccatttcaa actcccacat ggaagataaa atctgagtta gtccgctcgc cttagcatac
    13981 ttgattgggt aaaacacgtt tggttcattt gaaaatgcga tagttttagc gtttaggatt
    14041 tgtggcaaaa ttatctgcca gctagtccaa acatcagatt tccaaatcac taccttaaaa
    14101 ccgaaagtca catctttcca gcctttcaga atggtcaaat ctccttcacg tccatcaatc
    14161 tcaatactat caatgacacg ttccgtcact ggaataactg gcacttccgt aattcgcaaa
    14221 cctaaatcag tccgtgaatt aattgttccg tctaaaataa atgcgttcaa ttgaccaccg
    14281 cccatctaaa taactaaaaa aggaccagac aacaatgtct aatcctaaag tttataatct
    14341 atcatcctat tcggttgttt cttccagccc tattttcttg gtaatcgcta aatacgcgta
    14401 tacaaccagc acgccggaaa ttccatatat tgcatcgatt agatctggtg tattcagaaa
    14461 agtcatcatc gtttcacata caatatttgc aacaatcaga ccagctccca tcgcagtaag
    14521 aacccttaaa ttcgttccca taagcaacca aagatagcct atcaccaaat agaaaagaaa
    14581 gcttaatgcg aagtttgaaa tgtgagaatt aatctgattg ttgatgaata caaaaccatg
    14641 atttatgccg ctataccatc ttactgccgt taaaaataat ccaatagcaa gcagtagaat
    14701 atatcctatc agtgcaggtt tctttgcttt aaatttatgc ttcaacatat gattaccacc
    14761 atctcttcag taaacactcg ttagttatag tatacagtca aaatgtgaaa atccaacact
    14821 aatatgctac acgtctacga ttcaacgtcg ccaagctctt atcaatttct ggagcaagtt
    14881 tgccgaccaa tgttccatca ttcaagacga ctttaatgtc cattgcttta agtagcgttg
    14941 gcaacatttc actcgtgatg tttgttaagg catcaacctt agcacctaaa tcaagcatcg
    15001 agaatgtgct atcaacggca gcgtggatat cggtatcaat atcaaagctt ggcatataaa
    15061 gttttgttgg aatagctttc tgcatcgttt tagaaacacc agtcatttcc ttatcaaaac
    15121 ctacgccaat cccttgtgcc atgtacttcc cgatttcatc acgaaacagt gttgatggtg
    15181 aatgaatacc aaagaagtcc ttgatgttgt ctactacacc gccaaagaat cctgaaatct
    15241 tgttgcgtaa ccaacttcca gcatcagcga taccattcca caatcctgta atcaagttct
    15301 tcccgatagt tcccatagtt gaaacatact caccgaaagc attcgcaagc cctgtgatga
    15361 tttgcggtac tgctttgaca atggcagaaa tgattgctgg taagttttga actaatgcca
    15421 caagcaactt cacaccagca ttgatgattt tgtcgatgtt gtttaccaaa gttcctgtta
    15481 ttccgttgat aatttgcggc atggcattcg caatcgttgt gataatttgc ggtaacgcac
    15541 caatcaatgc cacaagtaac tgaatacctg cgtcaatcaa ctgagggatt gccccaatca
    15601 cggcattgat gatattttga ataatcactg gaatagctgc aacaatcgca ttgatgatat
    15661 ccggcaaagc tgtcacaagt gatgtcaaaa gttgaatacc agtctgaatg atttgtggaa
    15721 tagcactgat aataaagttc acgattcctg taataatctg tggcagactt tcaatcagta
    15781 ttggaagtgc tgtgataagt ccttgggcaa gtcccatgat aagttgtaaa gcagcatcca
    15841 acagtaatgg caagttttca atcaagccgt tcacaatcgt tatgattgcc tgtactgtaa
    15901 ttggaataag ttctggcagc gcttcgccta gtccttggat aagtgttgtc acaagttgaa
    15961 aggcagcgtc aatcagtaaa ggtaagttct caataagtgt ttgaacaatc gttaaaacgg
    16021 cttgaaccac cactggaata agtgtgggaa gtaagccaag tatcgtttca agcacttgtg
    16081 agaataatga ggttactgct tctaagagtg ttggcaataa ctgccccaat gcttgaataa
    16141 tggcatccac cactgccggt aaagccgaca cgatattttg aatgaccggt agaatattct
    16201 tgatgacgtt ttgaaattgt tccaccacat tgccaatcaa cataccaatg tcagcgctgc
    16261 tgttaccaag tccagccatc aagtttgaga ttgcagactt cataccgtca atcgaaccac
    16321 ttatcgtctc actcgcttcc ttggcggttg taccagtgat gcccatttcg gtttgaataa
    16381 cgtgaatcgc ttcagtcaca tcagcgaagt tcgagatatc atatttgaca ccagaaatct
    16441 tctcagcctc ttggagcaaa cgttccattt cggacttcgt gcccccaaaa cctaatttca
    16501 agttgtccag catcccgtaa ttttgcttgg cgaaaccttg ataggcgttc tggatggact
    16561 caatatccgt ccccatcttg tttgcattat cgctcatatc agtaacagct tgatccgcaa
    16621 ctcctgcggc tttcttggta tcaccaccga gagatgaaat caaggcagct gagaaacctg
    16681 tgacagtttc catgtattca ttggctgaca ttccggcagt tttgaaagca ttgtcggcat
    16741 tcttttgaac ggtggatgac gcatccttga aaagagtatc caccccaccg actaattgtt
    16801 catagtccgc gtaagcagat accacttgtt ttcctagctc cacggcagca gcgccagcag
    16861 caagtgccac tgtacccatt gctaccccga taccttttag aacgccaccg aacttttcaa
    16921 atttacttcc agacttttca gcctcgtcac ctgtatcctt gagttcgttg ccaagctcat
    16981 cagttttctt agcagcatca acttcttcag tacccatggc gtcaattgcc ttctcgttgt
    17041 tagcaacttc acgctccata ttattgagtt gggcttttgc attgtttaat tgcactgtcc
    17101 aattctgagt acgcttatcg ttttcaccaa aactgtcact agcgttcttc aaagcatttt
    17161 caagggtggt gattttttct ttctgggcat caatcgattt gtttaagact tggttgcgag
    17221 cagacagtga gctgatgctt ttatcgtttt tatcaaattc ggatgatacc agtttcattt
    17281 ctgaacctag caccttgaat gattggttga tttcacgcaa cgagttctta aactcctttt
    17341 caccttcaac gccaatcttt aaaccaaaat tatctgccaa ataaatcacc accttccctt
    17401 aaattccact cggtatcaca tcatcaatga ataactcaac tttcggcttg ctaatcccag
    17461 taaattgctt atgacactcc cataaatcca aaaacagtcc aagcgagcag taccagaaat
    17521 catctgacgt catacccatt tgaactgtcc catagtaata caggcgagta aatgtttctt
    17581 catcacttac tcgcctgctt tgttttttga atctgtttca ctttcgatat tgcgagctgt
    17641 gcccttgaac attgcttcag tgattgctga tttatattca gccaactgca acggactcgt
    17701 catcaactcc acatattcag tcgtgagttc atctttcttt tctgtgttct tcaagttgta
    17761 aatcttgatg gactgattcg ccaacagcgt aatcaaccag atgatttcat caagcgccaa
    17821 ttcaaagttc tcggatttca acaatttctc accaaggtta tcaagcccac cgtagtgact
    17881 ggcgatttct ttcgtggctt ttgttgtcaa aatcagttca tattcatctc cgccaaggct
    17941 aatcttggca ctgcgttctt cactcattgt tgcctcctag tctgctgtga aatctggttc
    18001 ataaacactc gtgtaccatc cattgatagt tgtttgttca actcccgtat cgttttcatc
    18061 aacttctgct ttccatggat gcttaccact agcatctaac ttgttacgac gcatgactgt
    18121 accctcaatt gttggtgttg agaatgtaat gtcgtcacct tttgttgcta ggcttgtgct
    18181 gggcacggca aacttcactc ggtacaacca caaatatttg tatttgccat tcgatttacg
    18241 agcacggaaa ccaatcgcaa ctggtgtgcc accatcttcg gttgtcgaaa ttaagacacc
    18301 gttttgatcc acggttgcac caacaagcac tgccgccaca gcacgtccaa tatcatcaac
    18361 accgagcgtt aactttccat ttttaaattc tttcactact tctgatgcac catcatcggc
    18421 gtataaagtt gcttcagcaa gttccaccga aagctcagct gagattgctt tcgcaagcat
    18481 agcgggtgtg ccgtatgttt cttcgccagt agttgccgac tctgtaatcg gtgcataata
    18541 taatttatcc aatccaatcg ttgccatttt cagacctcca tttcataatg cttggcgaca
    18601 tcaatcgcca catgatagta atttgtatct ttttcaaatc ctacaaataa tcgttcagta
    18661 atcgtaaact cgtgtttcaa caagttctga accagttgtt tttgcgtgtt cagatagttg
    18721 cctttcacaa acaaggaaat gcgaacttcc tgtacatcaa cggttggaaa gttatccccg
    18781 taaacatcga acctgtcagc gagtggtgtc agcacacaat acttgtctgg cgctttatca
    18841 ctaaactcgc ccgtttcaac aggaatatga agtgtggcaa aaagactgtt caattcctct
    18901 aaaatgttca aatgccgttt acctcacttt ccatttttga tttcattgtt tcaatgcatg
    18961 acttgcgact ttgtgatttt gcaggtttca agaatggctt tgctggctga ccgtgttttc
    19021 catattcgag aatgttcgca atcttagcat ttgaactgcc atcgattcga ggctcatcaa
    19081 aaccaacctt gatgttccag ttgccgcttt tatcttgacg agcttgtgtg ataccaaggg
    19141 ctgattcaag ttcaccagtt gaacgacttg gtacttttgt atttgagcca acaacggcag
    19201 ttaagttact tcggactttg ccttccacca cttcagcacc actttctaaa accttgggga
    19261 tgatttcatc tgtcttgttt tctagttttg aaacctttaa caagaaatct tctggcatct
    19321 tcatcaatgc tttagccatc cgcactcacc tccatttcag tagtcagcat ttcaaggtac
    19381 atattgcgat ttcggacatt ctccaccgac acaatctgat accttttctg atctgaaatg
    19441 acaaccatct ctgtcgtaac ctctagatta ggaatataac gaatccgaaa caggcaggtc
    19501 gctgaactaa aagtcgccat gtttacccat ttctcagtcg cattcttttc ctctttgtat
    19561 gcatggacag ctgcaagaac ttcatcttgt ttcactgaaa atccagcgct atccttttgg
    19621 ttcgtcactt taacgatttg tattcgttga ttcatctttc caaaactcat acttgccacc
    19681 gcctatcgag cctgagtaac agattgaccg tttcccagac ttgcttactg gcattgacgt
    19741 tatccgcaaa gaagccacca gttgaaccat cacggctttc gtaaaagtta ctggctaaca
    19801 caataaccgc ctgttcagtg gttggcggca tttcatgttc tgaatagtag ccagactcaa
    19861 tatgttgata gctctcagca taagaaagag cggtcagaat caatcgttcc aacagctcat
    19921 catcttcgtt atgctcaagt atgaggtttg ccttgacctt atttagtaag tcactcatag
    19981 gctactcctt aagatgaggc agatgctttc atcttcaaga tttgcactgc ttcaggtaac
    20041 acaagtttac cgtccacacg ttctttagct aagaaaccaa ccatgccatt acctgcaaag
    20101 agttcacgca actggtcgaa tgaacgcacg ccacggtcgc caatgttata gtagctgaaa
    20161 tcaccaaaag caatgactgg cgcacctgcc ttaacggttg gaacaaactc cgaagtataa
    20221 actggataac cgagcagacg gtcaggttca cctgctacca aagctggctg ccacatgtag
    20281 gcaccattgt tgtctttcaa cttacgaagc aagctgatgg tttggtcgtt catgataaac
    20341 ttcgcgtttt tacgatatgg acgtttcaaa gcatacacaa ggttcaccac atcatctgcc
    20401 gtaactgcac tttgagtatt agttgtaatg gcaacttgtc cgccacctgt tgctgcaaaa
    20461 ataccgagtg gtttaccaac accgtcacca ttcaagaacg catcttcttc cgcgtttgct
    20521 aaggctttgg tgaattggtc gatgatatag ttttcaaggt taaaggcgtt gtcataaagc
    20581 aattcatcag tcaccttgat tgccacatga agtttgtgag catcaagcaa gatttgatcg
    20641 aacgttgcat ccccaaagct caactcgcca ccttcttcaa tccatgaagc ggctggttta
    20701 gaacctgcaa tattaatctt gtgttcacca cttgtcgtaa ttgttgtcgc aagtgtacgc
    20761 atgatgtttt cagaagtcat gccgtcaatc aaacgtgagt catattcttc tggcaccaaa
    20821 taaccgcctg ccgtatccac accttcttga agaacatttg aaacatgtcg gaagtttgag
    20881 cgcaaggctt caagtacacc ttctttatat tcagctgaag cacgtcctgt tttcttaggt
    20941 tccattttat tttcagtagt cataggttgc gaagttaatg gttggctcat tggcttttca
    21001 agttcacgat ccatcgcttc cattgactgc atacgttcaa tttccatcgt aaagttcttc
    21061 acttttgttt ccatttccgc ataagtcttg gcatcttctt cagacatcaa gccatcttta
    21121 tcttgctttg cttctacaaa agcctttgct ccgttccaag cttgtgcacg tttttccatc
    21181 aattcttgaa ttttgttcat gggttagttc ctccaatttt tcattaaatt aagccgactc
    21241 attaacgaat cggctgggat agtgttatta atttcttctc gttcctttgg ttcaggtggt
    21301 tcaatacgac acttcttagc aatcgctgaa gtcagtgaat tagttacttt gacttccgag
    21361 tacatcgaat tattaaccgg aatatccggg tcaatctcac cgatgatttc atcggcaaat
    21421 cctaggtgaa cagctttatt tgcgtccatc cacgtctccg catccatcat atgtgccagt
    21481 tgcacacgcg ataaattcgt tttacgttca taggcgttga taatacttgc tttcacttca
    21541 tcaagcatgg caatggcttt ctccatatca actttttcgc caaacgcaac cgctgacgga
    21601 ttgtgaatca tcaccatcgc cacaggactc atgagtacct tgtcacctgc cattgcaata
    21661 acacttgcgg ccgaagcggc gatgccgtca atcttgactg tgactttacc tttgtagtca
    21721 gcaagcatgt tgtaaatact tgcggcagcg acacaatcgc caccacccga attaatccag
    21781 actgtaacgt tacctccacc tgaatttaac tcatctttga aaatctgcgg tgtgacttca
    21841 tcatcaaacc agctctcctc agcgattggt ccgttgaggt agagggttct ctctaggatt
    21901 tcctcttggt tgtcttgatt cagaaccgtt tgattcttcc acttccaaaa cttcttcatc
    21961 ttgttcctcg ctttcttctt tgtttacgcc ataagctgcc ccggcttgtt caattggcag
    22021 catattgcca ttgaagtaat atttcgcacc agcaccacca gggattgggt tcaagtcctc
    22081 aagttcacgc acatcatcag gagataggaa cccattttga ataccggttg catagccttg
    22141 ttgacgtgtg gcatagtcac cacgcaataa gccatccaca ttaaacttta tgaaatactt
    22201 ttctttttca tcgcgactta acagtgattt attaagtgct tgttcccaac gattgagcca
    22261 tggtgataat gagaacttca caaaatccaa actcaaatgt tcaacgtttg agaaggtcgc
    22321 gtggtctaag tcgccaacca tgtgaagggg cactcggaat atccgtgcga tttcttccag
    22381 ttgaaactta cgtgtttcaa ggaactgggc ttgttctggt gacatggaaa taggcgtata
    22441 cttcatgcct tcttcaagta ctgcaacctt ggccgcattt gaactgccac caaatgtcgc
    22501 attccaactt tcacgaagct ttcctgggtc tttcaaagtt ccaggatgtt ctaggacacc
    22561 accaggattg gcaccattgg caaaaaattt agaaccatat tcctctgttg caatcgcaag
    22621 cccgatagca ttcttagcca tggcaattgg tgaataacca accaagccat caaagccaag
    22681 gccaggaatg tgaagcacgt cagcttttga aagtctgact tgcccgacat cagcttgata
    22741 taaataatag atttccttgt tgtcatcgcg attaaccgtc atcttatctg gcattaaagg
    22801 atacaaagct acaacttcac ctttgccatt tcggataatt tgcgcatagg cgttacccca
    22861 aagtagcagg tgagtcatta aggtttcacg aaatacaaat gatgtcattt ccgaatttgg
    22921 ttcatcatgc agcaagaaat acaacgaatg tttcattgct ttatctttgc cgccagcatc
    22981 tcggtattgg taaaagtgta atggcaatcc tgccacggct tctgatagca cgcgcacaca
    23041 tgagtacaca gccgtcatct gcatagctgt ttgttcggtc acgcttttac cagctgatga
    23101 gccgccaaac ataaatcggt atggattcat gatggtttga tttgttggtt tatcgcgtga
    23161 tttgaatagt gattttatta tgcccattga ttgctccttt caaaatttga gtatcaaaaa
    23221 agcacctacg atttgtagat gctgtgttca cattagatat aaaaccgact tcctcttgac
    23281 aaaattatct caattgcatt atttaaaaag gtatttactt cctctaaaca tttcgatttt
    23341 tcgtcaggtg ttaaggattt tctttgataa ggaaagaatg aactacttgg tgcaagtttt
    23401 ttaaacgtca tataatcttc atcatcgaag aaatcttgta aataatttag ttctataaaa
    23461 cttttccaat ctttgaattt agagaaatag tctttattat taacgataaa tttatcagcg
    23521 ttagttgatg aaccatatcg tttatctctt acatcagaat aaaataaatc aggaaagtca
    23581 tgatacggcc ctttaccttt aacaccattg agagaaggtt ttgatacata tagtggcata
    23641 aaatttgcaa gagtatgact taaatcatag aattttacaa atttcacttt catctcttcg
    23701 gtgatatcat cggaatataa aatgactttc aaacgcccat aatgactatc tggcattgaa
    23761 aagttctttt gattatagca tttcaataac cgtatcgttg caccagcaat tgtgttgaaa
    23821 gaaataatcg tttctcccct ataattccca attccttcag catactttgt caaatcttca
    23881 gttagaattt ttccccacca ttttctataa aacagtctag aaattaatgt gttgtccggg
    23941 tctgaagata ttttatctgg tgctacttga atcaaatatt cattcataca catacacctc
    24001 caaagacaat tatatcaatg gattgtaata aaagctcata tttcacagaa acaacaatcc
    24061 cctatcatca taaacactgg caccattatc atttccacaa cgaattgcac gatccagagc
    24121 cataattgtc gcgatggcac catcaatctt ctcagtggat ttttctttat cagctttgat
    24181 attacctgcc gggtcagttc ggacgaaaat gttgtccata ttccaacgaa gaaccggatg
    24241 accgccatgg gctaatttct gttcaagagt caacttcatc aactctttag tagccgggct
    24301 catatccttg aaaccttgac cgaaaggaac tacagtaaat cccatacctt ctaagttttg
    24361 aaccatttga actgctcccc aacggtcaaa ggcaatctcc cggatattgt atttctcacc
    24421 cagttcctca ataaattttt caatgaaacc atagtggact acgtttccct cagtggtttg
    24481 tatgtacccc tgcttggccc acaaatcata attcacatgg tcgcgtttaa ctcgtaagtc
    24541 caaggtatct tctggcaccc agtaatatgg cagtatcaca aacttgtcat cctcatcttc
    24601 aggtggaaac accaaaacga aggatgtaag gtcggtcgtg ctggaaaggt cgagacctcc
    24661 gtaacaaact cgacctctca aactttcttc atctgcattg aatgaacaag cgtcccattt
    24721 atccattggc atccaacgca ctgcttgttt cacccattgg ttcaggcgaa gctgtctgaa
    24781 tgagttttct tcagctggat tttgtttagc cgaatcacaa gcagccacca ccttttcttc
    24841 tgcaatcgtt atcccaagtg acggattggc tttcttccaa acttctggat cagtccaatc
    24901 atcatcttgc ttgataccgt aaattaacgg atagaatgtt ttatcatgct ttcgaccttc
    24961 aataatgtcg agagcttttt gatgctgctc ataacaaatt gaattggtat cattccctgc
    25021 cgttgtaatc aaaaagaata gcggttgcgt tctagcatcc cctgcccctt tcgtcatgac
    25081 atcaaacagc tttctgttag gttgtgcgtg aagctcatca aatacaaccg cagaactgtt
    25141 taatccgtgt ttagagtatg cttcagctga taatacttga tagaaactat tcgttggctg
    25201 ataaataatt cgtttttgtg aagtcaggat tttcactcgt ttgcttaacg ctgggttctg
    25261 gcgaatcata tcaacagcta cgtcaaatac gattgaagcc tgcgctcgat cagcagcaca
    25321 gccatatacc tcagcgcgtt cttccccatc accgcaagtc aaaagcaaag ccacggcagc
    25381 cgctaactca ctcttaccat tcttcttagg tatttcaaca taagccgtat tgaattgtct
    25441 gtagccattt gccttaatcg ttccgaataa gtcacggata atcttttctt gccaaggcat
    25501 cagtttgaac ggtttcccga accactgacc tttcgtgtgt ttcaacaact caataaaggc
    25561 cacggcataa tctgcacgtg acttatcata atgggagtct ttgtccataa attcagttgg
    25621 tttgtaatta tcgattggaa ctcactccct tctacgcaaa aagcagctgt tgtcagctgc
    25681 cttaatctct ttattccatc atatcggcaa tgaactgaat ggcttcaggg ttgttcttgt
    25741 tatcccgcag caattgcacc agttcttgcc aatcgtagta tccttcttca atgccatctt
    25801 cattatctgg catgtaaatt tgataatcca tcatttatca ccctccaaga taaagcggct
    25861 gtactcatct ttgtgttctt caatgaaaat caccaactcg taaaagtgcc ggtcatgtgc
    25921 aattcgctgc actgcaatcg catcaaacat atttgtcaga ccactttctg caatctcaat
    25981 gatttgttgc ttaatctttg cgttcatttt gaccgtcctt tctgcctgcc tcataggccg
    26041 caattaaggc atcttccaaa caccaaaccg caatgtccaa gaagtcttca cggtcgctgt
    26101 tgtgggcctt caggtcgcca cgttcaggca actgaacgat gtaattcttt gcgatgttgt
    26161 aaatcttacg tttactcact ttcatcttaa agcccctttc tgatgtagat ggtatcttct
    26221 gtttttgact caaggtcaac ccagaccaaa tcgtggttca atatcatgtc cgtcacgtat
    26281 tgccaagcat cattgaaggt ttcaaatcct tctcttctac cgttttgaaa gaccaattcg
    26341 aagtctggtt tgatgatgtg caccacatct tcgccgtata ccgcgttcag tgttgaaccg
    26401 ttgtcccatt ggataaggat tgaaccaatg tcgtcaacac cggttactgt gccttgtgtg
    26461 cccttcgctg gggcatgtgg gtcgttcatc ttgatgagtt ctacacgact accttcaggg
    26521 taacgttttt tcataccctc gatttgttca tgagttggaa acataacttt gtcttccgta
    26581 ttcttttggt agtctatata tcactctaaa gggattttat agcaagtgat acgttattta
    26641 ttcaggattt tctaggtcat taaatgcgat agtctttcca tctcgaatca ctgaaacgtc
    26701 tgcatcgctt ccagcctgct cgatataacg cttaacaatc acatcacaga acttttcatc
    26761 cagttcgatc gtatggcaaa tccggtcggt ctgttcgcaa gcaatcaaag ttgatccgct
    26821 gccaccaaaa ctatccaaca cgatgcagtt gctgagtgac gaattggtaa tcggatatgc
    26881 caacagcgga attggtttca tcgttgggtg gtcaccattc ttctttggtt tatcaaattc
    26941 ccagatggtc gattccttac gtccggtgta ccattgatgt ttgcctttct tcttccagcc
    27001 atagagcacc ggctcatgct gccattggta aggcgaacga ccaagcacta gactctgttt
    27061 cttccagatg cacgtacctg ataaatagaa ccctgcatcc acgaatgctt ttctgaagtt
    27121 caagccttcc gtatccgcat ggaaaacata aatactggcg tcgttggcca tcacactttc
    27181 catacaagaa aaggcatcat acaagaagtt gtaaaatgcc tcgtccttca gattatcatt
    27241 tttaatcgtt cccgctttac cttgatatga gaccccataa ggtggatctg tcaccacaag
    27301 atttgctagt ttgccgtcca tgagtttctc ataagtttca ggttttgttg agtcaccaca
    27361 aactaaacgg tgacgaccaa gcaaccacaa gtcccctgtt ttactgaagg ctggcttttc
    27421 aagttcctca tctacatcaa agtcatcatc ttgggcatca tcattaccgt tgagtaattt
    27481 atcaagttca tcagtttcaa aaccaagcaa atccaaatca aactcttcac cttgcaggtc
    27541 tgataactca accgccaaca tttcatcatc ccaaccggcg ttcattgcta gtcggttatc
    27601 agcaagtatg tacgcacgct tttgtgcctc ggttaggtgt tccacaaaca cgcacggtac
    27661 ctgcttgata ccttcttctt tagccgcaag aatacgtccg tgacctgcaa tcaagttata
    27721 atccttatca acaatcaccg gatttatgaa cccaaactca cgcatagaag cttgaatctg
    27781 tttcacttga tccttactgt gggtacgggc atttctggca taagggacca gcttatcaat
    27841 atcaactttc tcaaaacgtt ctgttgtgtt cattagaaca acccccattc tgctagtttc
    27901 tcaaacccgc caagctcatt cacataatca cgcgcaactt ccacgatttc agaataaggc
    27961 acgccatcaa tctcagtgtc accaatcgca cagcttaact caacgggcat accagtttct
    28021 tgagctttca agaatgcgta gatgttcact gacacatcag cttttgataa gtctttccca
    28081 tggataccgc cacctgttac tccgtctgcc atatcggaac caagcttgcg attagtcgca
    28141 cctgtatcca catccgggcc gccagtccaa tcacctagag ggttgactgt agcgtttgga
    28201 tatttttgtt ttaactcatt tgtctttgcg tggctctgac aaattatcaa gcgttcacca
    28261 tcaagaatgt actttccatc aaatggatgg tcctgatata agtcacgcgc aatttttgag
    28321 agctgtaact gttcatcagt caatggcatc ccacgaaaga ttccattatc gccacaacga
    28381 atttcagtcg cttgattgtc agccaactct ttatcttggg gcacaatcac cacatctttt
    28441 ttaattttgc ctgcgatacg attaatcgct tggctgattt cattcaaatt taaagcagtt
    28501 gaagtttcaa taatcacatg acagatgcca tgaccaatta aaacctcaac tgctatcttt
    28561 ggattatttt cttctgcgta tgccaagtcc acaatggctc cggcaatacg atctgcaact
    28621 ttatctggat gttgcggatt aaccttctct atcataaatt ctgttccttt caaccattct
    28681 tagaacgtaa caatcgttcc atcgcatccg tattcggatc tcctgtgtat tccactgatg
    28741 cattctcttt tacaatctgt tgaatctcat accacgtcac atttgcttgc ttttggaagt
    28801 tctgcgacat cgccacaaat ggggaggcca tgactgcctt ggtagtcggg tgttttccga
    28861 gcaaaccata ttgactcaga gcattttcac attgaacaaa acgcgcaaag ttctgagcgt
    28921 aggactcaac cattcgtggg gctatcaacg tttcgcatcc gcggtctttc aaccattgga
    28981 acgttgagtt ataaatttca tcagcgccta gcacagtgcc atccttctgt tttgcagaaa
    29041 ggtaagcact tggttcagga taatcattgc cctcaagctc tacaccctca ccaatatctt
    29101 caccaattaa taattcaaca tccggtaagt caaattccat ccgcgatgct ttctttccag
    29161 cagcaacttt atcaacgagt ggctgtgagc gttcccctga acgaggtcgt ctgccaccgc
    29221 gttgtgttcc atcttttgcc aaaatttatc acctcttttt cttgtttggg gctatccccc
    29281 gtttgaactg aacttttttc gcgtaagggc ctagcgcgct gtacagttta gaagggttta
    29341 gggataatta ccgcccctcc cccccttgtt atttttcaca tgattgtttt atcttttgtg
    29401 tttcgtcgag tcatgtgaat tttcgagtga catgatttgc agacactcat caaattattt
    29461 tcatcatgcg taccaccatc acttaattcc ttgatgtgat gcacaatctc aaccgaagta
    29521 aagcgtcctt ccttcagaca catctcacat aggggatgtg ctgccacgta ccttgcccgt
    29581 attttcttcc acgctctgcc gtaacgtttg ttgattgccg gatcacgctc gtatttctcg
    29641 taacgtttcc tcgcaagtgt tttatgttca tcacaataag tatcatgcgt acggttcgga
    29701 caacctggga acttacaggg gttagctggt ttcattggca agtggggtca catcctttcg
    29761 ggcaaaagaa aagcccttga aggatttttc ccacaaaggc ttcgagtatt tgttcgatac
    29821 ttctattgct ggtattgctt gagtatcgtt tgtctttttt gacatcttaa tgatatcact
    29881 aaaggctact gacattcact gacacatttc aaatgactat gggttcatct ggtagcttca
    29941 agttctcaag agctttgcta tgccaacgaa agactgtccg cacgtcagcg tgaagatctt
    30001 cagcaatctt gtcccagctc tcttccaaca ggtatcgatt gattaacaca atcttttcgt
    30061 ccacgtttgt gagttcatca atcacgccac gcatttgtcg tttgaggtct acaaacttat
    30121 caatctctgc atcaatttca gcttccaatt ggtaacactt ctcaaggtta cgaacaaatg
    30181 gtgcctcagt attccgtgtg ccacttgaaa tcttttcttc gaatgttgtg gaagaaactg
    30241 tcaccgccag ttgatagagc tccgccttct ctttaatccg gtcgtcaatt cgtttattca
    30301 aacggtacgc ttgtttcaag tattcttttg gtgtcacttg cttccctcca atctagcttt
    30361 cactgcatca atcaaacatg attgtgtttt atcttttcgt ttcaaagcca acatcacatc
    30421 ttcatcaatc gtatctttcg caataatgtg atgaatgacc acagtatcac tttgcccttg
    30481 tcgccacagc cttgcattgg tttgttggta aagttccaaa ctccacgtaa gaccaaacca
    30541 tacaagtgtt gaaccaccag cttgtaaatt caatccatgt cctgcactag ccggatgaat
    30601 gacagcgatg gggatgctac cttcgttcca atcaataatg tcctgagacg ttttgatctg
    30661 tcgaacgttg aatcgttcct tgatacgctc cagatcatgc tggaaccaat aggcaatcag
    30721 aatcggtttg ccatttgtac cttcaatcaa atcttccaaa gcatctaact tctggtcatg
    30781 gatgtgatga ctcacattaa actcatcata gattgctcca ttcgccattt gtagcagttt
    30841 attcgaaagc accgctgtat tggctgcatc aatggtggct tctttcaatt gtagcaccaa
    30901 gtctgcctta atttcatcgt acttttgctt ttctttgagt cccatatcaa ctggcacttc
    30961 attcatcaca agctctggca tatccaagaa atcgactgac ttcattgaaa tcgtgatatc
    31021 tgaaatcttg tcgtagattt cttgttctgc atcttccctt ggtttccaag aataaatctg
    31081 catgccattt tgcttgtcag gtagaaagta tttatctcga taacgtgaaa tataatagcc
    31141 caatctctcg cccatatcca gaaccttgaa ctccgcaaac aaatccatca aaccattact
    31201 tgaaggtgtt ccggtcaatc caacaattcg gtcaaccttg tgtctgactt tcataaagct
    31261 agtgaaccgt ttagctttta agtttttgaa ggacgacagt tcgtcaatca ccaccatatc
    31321 aaaatcaaac gtaaaacccg atttgttaat cagccagtcc aagttctcac gattgatcag
    31381 atagatgttg gcattatttt gtaatgctgc cattcgctga cttggtgttc caagaaccac
    31441 ggaatacgat aaatctttca aatgatccca cttttgaatt tcagttggcc atgttgattg
    31501 cgtcacgcgc aacggtgcaa taattaagac acgttgtatt gtaaagtcat catgcatcaa
    31561 gtctttaata gctgtcagcg ttgtcaccgt tttacccaag cccatgtcca atagaagggc
    31621 ggcagtttta tgatctttga taaaatcaat cgcatatttc tggtagttat gtggtacgaa
    31681 cttcatcaga catcacctcc tattctttct agtaactcag gtatctgatc aatctcatca
    31741 agcacgaaac actgaaaccc taaatctgtc agttgtttca tacgaaaaag ctgtaaagac
    31801 cgtggcttct tccctggcgc cttcacttcg acaaatccaa gtttcccatt tggcataagt
    31861 accaatcggt caggcacacc agcaagcccc ggtgatgtaa acttcgggca aataccacct
    31921 acccttttga cggctttcac tagtgcttgc tcaacctgct tttctctcat agccacaccg
    31981 tcaaatactc gaaatacgca tcacgcaaga tttccaagta ttccttttct gcgcagtgaa
    32041 tcatcagatg tgcgacttgc ttatcgaatg atttcttcca aggatacgtg acatccgaca
    32101 tggcaaactc gtaaaactca tcaaatcgta aatcacgttt accgattcca atttgtaacc
    32161 actgtttaaa tttcatttag atattctcca atctgcaagt atggtgtaac ccgtgaatac
    32221 gcaatacata actttatata ggtggtaatt ttttatccct taagaaaagt tctatataag
    32281 acctacacag gttacacttt cctttaaaat caactattcc aagaactcgg atttgagttg
    32341 caaaccaaca atcttgtttc ctttattagt tcgccggcgc ttaaatccgc ccgcctccag
    32401 agccgtgtaa aaatcggctg aactacgaat gtactcacca gttcgcgtac aaaacgcacg
    32461 atactctgca tacaactcac cagatttttc ttcaaagtca acagcagtct cgcagcactc
    32521 gtctaagaaa tggccaagcc aatcattcgc cgtcttatat tcagaaattg cattagcgac
    32581 aacttgtgga accgttaaat gaaagtctgc ggcgatgact tttttcgcac cgtcaaggat
    32641 ccattgcaaa atggctccgc cggcattttc aaaaaggtag tccgcataat tcttgatgtc
    32701 cttattgccg tcaatcgtag caaggaatgg aatcacaatt aaacgacgcc aggttccttt
    32761 atcaatcgct ccaacttttg ggaggtggtt ggtatagagc accaaagtat gcgttgggac
    32821 atacttgaat gggtctttgt atttcttctc agccgcaatt tcatccgtcg agcagagctg
    32881 cttaatgtta gatgtgttga gtcgcatccc ctcttcaagc tcggctgcga tgagtaggcg
    32941 tttccccttc gcttccgcaa gttcaggttt cacattgcgt cggatttgac ttgtaagaat
    33001 atcagctgag atgctgccag agtaattacc gagcacacgg ctgatgacat tccagaacgt
    33061 ggacttcccg ttacgccctt caccatacga gatgataagc gcttcaacgt aaactttacc
    33121 aatagctgca agtcctacaa tcatctggac ataatcaatc aactcttggt cacctacgaa
    33181 aatggtgttc agagcgtcca accaaatctg ctgattggcg tcactcgggg cacattccgt
    33241 ttgcttcgtg atgtaatctt ctgagttgtg gtcttgtgat ttgcctgttc gcaaatcaaa
    33301 ggttgctgtt ggtgtgttca gcaagaactc gttcacatct agtaaccgtt gctcgatttc
    33361 aagcatgggc tttgcctcgg ttaatgctga cgagagatac ttgctgtcgc ggcgtttgac
    33421 agcaaacttc ttatactctt gtgcgtgttg gtacaaatca aaactatgac gttgcgctgt
    33481 gttgaacatg gtgactgcct ttttcggacc aacactagca agtacatcga acgcgccatt
    33541 actaaccatt tccttggtac gcttctcaat ttcggtttca gattcttcca gttgacgttc
    33601 tgtcaatgcg tggatcacac cttgcgcttt tggtttcgac tcttcccaat agctgccgtt
    33661 gtagacaagg taaccagttg acggtgagta atgaagctta cattcatact cgcgagctaa
    33721 gaccgttgct tgacctaagt ccgagtagtc ggttggctca agtgataaat cagagttgta
    33781 ctgctctgga ggaatgtagc cttcttgttg tttcactttc ttcccaaagt tgaccgcgct
    33841 attccagata gctttaagct cctcctccgg cagtggcgga ttacattttt gggattgttc
    33901 gagatacttg tgatacgtat cttccgttgc accgtagcgt ttgatgagct ttcctgcgat
    33961 gtgactcatg cttgaattac ggctgccttc gctaatctct gactgcgcat tatcccaatc
    34021 agcaaacaga tcatcttcga gaaaatcagt gatcaactta ctaccttcat agatttcaac
    34081 ttcagagttt ggtgttccga aaagcaaacg tgccgcatcc atcgcattgt tgtcaaagta
    34141 tgggaactca ctttgaatct gcttcttgag ttccacatat tcatctttat cagttgtggc
    34201 aggaatcggg aaatatacgt gaaaacgtgg acgtgctgat ttgccacctt tgtctttcat
    34261 gttgttacgt gaatagctga ccgcaaacgt cacatcagga aactgcatcg ccacttcgaa
    34321 aggggctacc caatcatctg gattgtctga gtggtcattg tcgcaatcaa gtggaatcac
    34381 atcagactgg atgaagttgt cacctttgcg atagttgcct ttgtactcag ccatcacatg
    34441 atccagcttg accgtggcaa caagtgtttc tttatctgtc accacttgct tggttgggaa
    34501 caatccattc ttggaattac ccacctgatt ggatgtgaat aacgtcatct taagcatttg
    34561 agacctcctt catctcatca tcgaagtacc tgatatgttt cttccaatac tccgcttgtt
    34621 caatttcaac tgccatgcct tgggagatgg tgtcacccac cacccagact tcctcacatt
    34681 tgcccatgag aacaagtgac atgtgcattg ccacatcacg ctccgttgcg tcgtccatga
    34741 attgtggata catcagatgt gccgcaactg gcatgtagcc acggtcaaga gcaatacggc
    34801 agtaacgctt agtgttttta tggttgacga caacatttcc tgagaacggc gagcagacat
    34861 agactgatgt tctgtaatct ttacgtctag gcattattat cacctcgctc aatcactggt
    34921 aagatgttct tcttcgattt cagcaagtcg tacaagaaca aacgaccttt ctgcgtccag
    34981 taggtatgca tcacgctgcg ctctgcatct atcgcatgag tttttgattg tgtgtagcct
    35041 tgattggcat acttctgata aagcaaccac atcttgcctt gcttgtactg aattcccagt
    35101 tcatgaagca acttgttcat tgcctgtggt gacataccgt agtccttcga gattttagaa
    35161 atcgacatca cagaattgtt ttgaagaatc aaatcgtagt agctaacttt tggtttcatc
    35221 tccaaaatca tttgttgttg catcgccacc atttcttttt tctcttgtag ctgcttcaac
    35281 actgaaatgg cccatgctgg atcttcgatt gactttgtga tgaaatcttc tgtcgcgtac
    35341 acaccgtgtt ggcggataga cggcaagacc tcatttgtca cccaacgctt gaatttcttc
    35401 gcgttcggca tcttgctaga aatgatgagt gagtacaatc cactttcgtt gataaggata
    35461 gccattgtcc cattaacgct gaacgattcg ttcaccgtct tatcttcctc atcaacatga
    35521 tctcgtaatg ctttttgtgg atttgtgtag ccaagaattg atgctacatc tttaccaaca
    35581 aagtgaggga catcgccaat catcaccgtg cgtacagagc caaactccgc attttcaaaa
    35641 actttcaatt cgttcatctt gtgaacctcc tgtaatagaa ttttcaggag aggcattttg
    35701 ccccttctaa cttacaggcg aagaaaatgg tcgaaagtaa actctgactc gaatttattt
    35761 ttgatttttt tgccttcgtc tataagcgaa gaaagaaccc aattcgaacc ttatttttga
    35821 taaaaaaatt tgaaaaaaat atggagccat ttctgactcc acattgacta atccttttga
    35881 tagaattcac actcgtatcc atcagcgttc agtagcaagc cttcagccca cttaggcgtt
    35941 tgactcatca actgacagat gtcttcaacc ttcgattcca ttggcacttc gataatcact
    36001 tcgtcgtgga cgtgcccgac aatgcgatac ttacgaagat tctccattgc gaaacagaga
    36061 atgtctctag ctattgcctg cacgatgttt tcgacaaact tgggaccata gctttcaaga
    36121 cgttcccatt tcttggtagc accgacacct tcataggtaa cagcttcacc gccgaatttg
    36181 ttctcaccaa tctttggttt tacataaact aatttacgtc ctgacaagag ttggatgaac
    36241 agaaaacccg acttgtattc aaacttgatg ccatgcgtcg ctgtgctccg tcgttctttc
    36301 acacaagtct ttactgcctt atcaacagcc caccaaagct ccgttatgtt aatgtttgag
    36361 tctcgccaag aattaacaag cggctgtaat tcatcctcag ttagtcccat ttcaagagca
    36421 tccattgctt tcaatgcacc caccgatcct ccgtacccta gagcaagctc agcaattttc
    36481 cctttttgac gtagatgccc attcacgcca tgcttttcaa ctggcacacc gaacatctga
    36541 ctggcagacg cacagtaaat atcgccacca tttttgaata cttcttgacg ccacttctca
    36601 cccgacagcc aagcaatcac acgtgcttca atcgctgaga agtcagatac gataaatttc
    36661 atcccttcac gtggtacaaa cgctgtccgg aggagttctg agagtacgtt cgggacattg
    36721 ccgtagagaa tgttaagcat ttcaaaattg ctttgtttta ctaaactacg cgcttcagat
    36781 aaatctgaca tatggttctg aggcaagttt tgaagctgga tgccacgtcc tgcaaagcgt
    36841 ccggtacggt tggctccata gaattggaat aaaccacgag aacgattatc agaacacgca
    36901 aagttttgca tggcttgata ctttttcacg gatgacttgg cgagttgctg acgtaagacc
    36961 aggacttgct ttaaattttc atcaataggt tgttcgagta gttcggccac tgccttttta
    37021 ccgagtgact cggtttcaag tccgtggtct agtaaccatc cccgcatttg aagcacgctg
    37081 tttggatttt ctagtccagt taattcttgc aattgttgat tcaatccagc ttttgataat
    37141 tcatccaact gaacggcagc ttccacaaag tccttatcaa tctcaattcc tcggtcgttg
    37201 atttcctgat ccagccagta ttctttccaa acagaatcaa gcactggaaa attggaaagt
    37261 tttccttgga tagctatttc aacttccaca tcacgactat tgtatgtctt aaatgctgac
    37321 catttagttg gcgcatggtg tggtaagttc ctcgtgcgtc caccattaac tttggtcgga
    37381 ttgcatggca cgcaaaagta tcggatgagg tcttttcctt ctgtgagctt ttgtttatcc
    37441 aaaccaagaa cagcaccaac tcctacaagt gacaacggca aacctaatgt cgcaccccac
    37501 accattgtgc attgccaccc tcgtggattc aaaaagccat ccaaccccag aaagcgtgac
    37561 aaacacacac gttcaaaact cgcattgaag gcgtgtttaa tcactgtttc atctgatagc
    37621 gaagttaata tttcagccgg gatagtttcg tccatcttca gatcaaccac ttcgacggga
    37681 ccaccatcta cactgtatgc aaatagcagt atttcaaaat cgggggactc tgaatatttg
    37741 tagacaccac atttcgataa atcgacacta ctatacgttt ccaaatccaa tgacaaatgt
    37801 ttcattttct tcctccaatc aaacaggcga cagcacttct gccatggccg tttttgtttt
    37861 aatgattaag ccaagaaatc atcatccgcc aaggtttcaa actcatcgct tgcagatact
    37921 tgtgagccga gtggttcgcc gtcacgtaca aactggatat tcccaagccc tgccgctacc
    37981 ccgcgatttc catttgcgtt aaaggcatag aaattaatcg acacacgagc gtagcaacca
    38041 ctatacactt gtgaacgatc caagattggt tgaacgtgct gatccacgat ttgtggtgca
    38101 gtggttgagt tcgcattaat gaagtaatgg ccttggtatg cttcatcgtc gcgttcgata
    38161 tccccatcac gtaatggcag cttgatttct gctttgtttg gtttcttccc accgaatttc
    38221 gcaagtccct cttcgattgc tgcatcgatt gctttttcaa tggtatcaat cgttctctta
    38281 tctgattttg gaatcaagac tgctgttgaa tatttctctg cgcccccgtt gatggatgct
    38341 ggttcccaac cgtggaagta tgagagacgt gtgtttgtac cagtgattac tttgcttgtt
    38401 ttagacatat tattgttcct ccgttaatgg tttaaattct tcgtttaagt tgtttactgt
    38461 gaccgcttgt cgtttatctg aaccatcgac tagcgtcagc ttgccttttg gtttgatgat
    38521 gtattcgccc aagatttcat tgaatttctc tttaccgagt gccttcgtca ttgctgtgac
    38581 accaagtaac tttttatcat atgggtcgta tccagccttg atgaccgccg cagcgacatc
    38641 attgtcatta gaatacttac gagtagagcg accctcgact aatttgtgat gagtccattg
    38701 tttgccaccg agtgcaagtt tcaaagcaca gtccttgata tcgttactcc aagaaaccag
    38761 gtcatccact ttaccgatga tttcttcgat ttcatcatca ctaagaagct ctggtggttt
    38821 gaactcatat tgcgccagtt tcaagttatg gtctgctcgt gcacgacagc tattcttcgc
    38881 cggacagaat ccacaccatt tcccgcattg gtactcaccc tcacctttga aagctaaatc
    38941 tgcggcaggc gacaacacat cttttgccca ctgaagtaat tcttctttca gcagtgtgta
    39001 cgtactcaca ttctcacgcc ttggctggaa aatggtcatc ttgacttctt gaatgtcata
    39061 aataacatcg aaaagctcca acgctcccaa ggcataacac atcatctgtg ggttctgata
    39121 cgcatctacc aaaattccta gaccgtattt catgtcaata acagataggg ttccatccgc
    39181 aatgacgaga cagtcgccgg tcccaaagcc gtctgggacg taccttgaga agtcgagccg
    39241 ctgttctact attacaatag agtcaactgt tgtctgtttc gccttagcga cttcttccat
    39301 gacgaaggtc gcataacttt cagcacactg ctccatctcc tcgtcgtaga attcaagcgt
    39361 agatgttggg tctgtgacgt cattaccaat gaacttattc agtttgtact cacataaggt
    39421 atgagcatca gtgccttgtt gggcatacgg gctcgacttg ttttcaaagt gctctgacaa
    39481 gcgaacactt ggcggacagt gaatccaccg attacttgaa gatgcggata ggactgcgtg
    39541 ttgtgttggc atctacaatc cctccgcatc tgcaaccaaa gccgcgtagt tttctgtcgg
    39601 cacatcactc aagcgactgg caccatattt ctcaatcagt gctttcactt cagttgactt
    39661 accttcttga gatttacgtg ccaacaaacc acggacttct tcaagcgtcg gttgtttgac
    39721 ttctgatttt gtttccattg tcggttctgg tgtttcaact tgtggttcag gttcattggt
    39781 tttcatcgcc acagccaact cttgtaagct atcagctaac gcttggacgt cctcaacgac
    39841 ctgaagaaat agttttgttt tactcacgtc attacgctcc cttcttgtta acttcacgga
    39901 tttcgactga ttcgattgtg tcccctggtg tgagcacgag aattttgtcc atgttgccct
    39961 taccgaacag ccaccgtgca aaccagcgag gcaaatgacg cacaccacct tgtaggacag
    40021 tttctttgtt acccgtgtca tcagtcacgt ttactcggat tttgtgtttt aggttcatga
    40081 tggtcctcct tcatctttat ttgtttagga tctactccct tacacaatac aggcgaagga
    40141 aaacggggaa agtaaactcc cccgtaactt ttttctaaaa taattctttg atcttgtctt
    40201 tgatgattcc aaaggtgcga ttgattgact gttttttctt gtcaagcttg tccgccactt
    40261 cagtctgaga catgttttca gtgacgagca tgtgatagat ttcttggtgg tacggtaata
    40321 attgcgtcac gacttcacgc aaacgatcga tacgaggatc ttcttcatta tcttggtaag
    40381 catcgtaaac caatgattgg atggggttgt ccccctcatc gcattcatca tcaaattcat
    40441 tgagcgacaa attgtagtta cgtttgaagt aatcgttggc ttcaccatcc acgtattcgt
    40501 ccattggata atagccgtgc tcattattaa agtcttcacg gaacttctct ttccaagcct
    40561 tgatcttttc tttttcttct tctgtgcgtt caggacgtgc gtttttataa aagttgtaaa
    40621 tcgcacggtc gtcagcgcga tgcagctcct gaatgttaag ctcagtgacg ccgtcttcgc
    40681 ctgggcgaat ctcaaccact tctttttgga tacgtccatt ggcatcagta cgctcaaaca
    40741 cgtacttgta agtgctgcgg tcgttttggt gtgttttcat aaatctactc attagatccc
    40801 tctttcttgc ctgaaagagc ttttgagcaa ggaaaacaaa cagggaccgt gccaatacgt
    40861 acaccgtccc tcatcacgaa aaaagagcgc aataaagtaa ggcatacaat atctgaaaaa
    40921 ttttctggat aaccagttct tttcagtatt tgtaaccttg ccctattgca actcaggcat
    40981 tgttatattt tattagtagg actattcgct tttaagataa attcgttcac tttcttgaaa
    41041 cataatgtag tgtttatgaa ccataaatga taaaattaaa tagttaaact actatgtttt
    41101 aattatagaa aaaggcctcc cttgttttcg tcagtcatcg gcggcaaacg gcggtcaacg
    41161 gcggacgtaa caaaggaggt tgtattttgg aaataagtga aattactcaa acgctttatc
    41221 cgtttatggg aagcgagtac gtgaagccac aggatttttt aattgcttta ctggatagtg
    41281 tttcaaatga tgacgaggcc accattttcg gtttttctga tgataaccta cgaagatttt
    41341 acaatggtac tcgaccatta acacaagagt atgcagttcg ggttgctgga aaattcaaca
    41401 tcgacaaatg cacgactttt atttatgaac gtcttataga tgatgctcca accgtcctta
    41461 agcaacaatt gattgataaa ggtgtggtaa tccccaaaga tacagacgtt tctgaaatag
    41521 tggcccatct tttacttgat gaaatcacct caattgcttc tcaaacaaaa gtaaaaacaa
    41581 aaaaggccaa gccgaagctt agcgaattag agctcgcaag tttaagaatc aacactaatg
    41641 gtgatttctc ttataatgat gaagtgaaaa aattctttga cgatttgcca gttccccttg
    41701 atattagagc tgaggagatg acgtatgtca tagctctttt ttcagcatat gcagatgccg
    41761 atggatacga agtatacgag catacaaacg acctgccaaa ttcgcggcta aaggatttca
    41821 agagacagcg aagaaattac tataaagctg aaagcatacg tcgtggggtc cgtgataact
    41881 ttacacaaga ggaaggaact gagcactttg aagctttaaa agaagatatg tacgacggca
    41941 tcgaggaagt ctatgaagaa gactacgatg atggcgttga gagactcaaa gccgttctac
    42001 agcagtcgtc tgttataaca ttaaacggat caatcttatc gtttataccg ggattgctgc
    42061 aaaattctgc taaaaaaggc atttgccaca tgctggttaa tgacggaaag atcaaatggg
    42121 tgactgaaga tgaatagttt gtttaatact ccatttgaaa tttcggttcg agtaatgtta
    42181 ctgctctctg aatttaaaga gcctgtcagc gctgatagaa ttttaatcac agacttcatc
    42241 accacgtatg gtcgcgactt tgaaatttcc gattacaact taaatggtga taacagctat
    42301 agattcagtg aatttattgc gcgacgtgaa ttagttaaag atgcagtcaa aaatcttgta
    42361 ctgcaacatg ttatccaacc atcgcaaagc aaagaaggtt ttaagtatgc tttatcagaa
    42421 gatggcctaa agttgatatc acaattcgta tcggtatatg cagaaaaata cttacaaata
    42481 gctgacgaat ctgttaaata cattcaagat aaaagtgacg ttgagcttct gcgacaaata
    42541 aaccaacgcg caaaggaatt taaaggagag caatgataat gcaaaattat tatattgaac
    42601 gtgttaccat aaaaggcaaa gatcgtgaag atgcaactgt caagttcacc aatggactaa
    42661 acataatttc tggcccttca aataccggaa aaactagcat tgttacggca atcgattttc
    42721 tctatggcgc gaaaaaagac gctgctccct tttcaaccga tgcaaccgga tacagtaaag
    42781 ttgaactgat tattcaatcg actaatggca aatattcatt aactagagaa tttgatagca
    42841 actccatttt ggtatccaaa ataacgagtt caggtgaata tgtagattat gaaaaatata
    42901 atgcacgatc tgggaaaaat ccaattagcc atttttggtt acaactttta ggcatcgatg
    42961 atgattatca gattatcaag aatgaggatt tcaaacgtca aaaattaact tggcggacat
    43021 tagcgcctag cacacttatt ccacaagata gagtcaatac cagcgcctct gttctttttc
    43081 ctggggaaac aacccaacgc acagcttttt tatcatcgtt gttacttctg ctatacggtt
    43141 cagattttgc gaactcggaa gaaaacgaaa caaaagaaca taaaaaaatt agaaggaact
    43201 cggtgattaa ctacatcaat tcaagccttt ctgaattata tgaaaactac gaagtggcta
    43261 aatcagaatt acctgaggct ccctctgatg attttcaaca aattgtcgat ggaatgttcg
    43321 ctgaacttga aacaattgaa aagcaaatca atgatgcgtt agccagcagc aaaatcttga
    43381 taaaagagat ttatcaaaag gaacagtatc ttcatgaaac tcaacttctt ttgaataggt
    43441 acaaacagtt acattatcaa tatcaatcgg atattgaacg actaacacta atcgtagatg
    43501 gtgagcgatt attaaatgat cataatgaac atgatacttc acatagctgt ccattctgta
    43561 acggcgaact aagtaaacct gtccaaacaa cctacatcga tactgctagg tcagaattac
    43621 attcattgct ctctcaatta tcagatttga cggaagaatc tcatgatacc gaagttgaac
    43681 tgcaagagtt aatggaacag cttgatgagc ttaaaacaag aaagaaatcg cttgatgcag
    43741 caatcaacga tgaactaaaa ccgaaagctg agacgttaaa ggatcgaatc aataaaatcg
    43801 aaacttacag ggatattaaa caacagctaa tctcgatgga aaaaatgcaa aataagctta
    43861 aaagtgatct tgaaaaaatc gagaatgagc cagaagacga acgtatcctt tataaaccta
    43921 aagagaaatt tgatgcagaa ttctacaaag aaatgtctga gttactggac tccatgttac
    43981 gtaatatgca ttataaacct aatgaatttc gtaacgcatt tttcgccaag gatattttcg
    44041 acatcaaaat caatggaaat ctaaaatgga ccgaaaacgg acatggatat acagcgttta
    44101 tcaattcagt agtggtaatg gcatatcgac atctcttgta taatcacgct aagtacacac
    44161 cttctgtgtt cattatcgat tcccctcttc ttggattaga tgaacgcaaa aaacaagatg
    44221 gcgatgacaa tatgaaagaa ggacttttca aatactttat ggatcatcaa aacgaaggac
    44281 aacttatcat cgttgagaat acaacgaact tgccagcact aaactatgat gataaacaag
    44341 taaatcagat tgtctttact gaagataaaa acaatggacg ctatggatat ctatacgata
    44401 tttataacta gaacaggaat gattacatga agtttagcta caatccgctt tggaaactcc
    44461 taatcgacag aggactgaat aaagcacaac tcagaaaaat gactgatatc agttcagcaa
    44521 cactctcaaa actaaataaa ggcgaaaatg taacaacaga tatcttactt cgcatctgtg
    44581 aagcacttga ttgtgattta tcggatattg ttgaaatcgt tcgcgattaa atatcaaaaa
    44641 tgcactgtat tcgattcatg agtacagtgc attttcatat ttatttttgt aatccagcga
    44701 ttacttcatt gattttcgat tcaatatcat tcaatggaac catataccat tcagtagctt
    44761 ttttgaagtt tccatcctga aagacaactt gtttgtcctg gaatacatga tggagagtgc
    44821 gctcaagtag ctgagcgttg acattttgta cttcagcaga aagcatgacc ttcactggtg
    44881 catttaagta ggtcacgtcg ttttccgcat tggcaattct actttcaaca tcatttcgag
    44941 tgaaaccaat cttatacagg ttattccact tgctaatatc cggattcgtg cttaatgact
    45001 tcagcacgta gatataacct gtcgtaaact cttctccaaa gctttcagcc agtgtttcct
    45061 catctaccgg ctctgtcacc atccttccat gacgttcagt cgagtataga gacgaagcta
    45121 aagattgtaa caacagattc ttattctctg tcccattctc ataaacaaca tgcattctgg
    45181 catttgtttt cccattatca gcgataaagg tatccttcga aatggaaacg atatacatca
    45241 ttacgccgtt ttggatgtag aatctatttt catgaatatc atactgctca aacgggataa
    45301 tttttcgtct gccagatgcg atatctgcat gcacttgatc gaacaagctc ttatactttt
    45361 caaaatgact tgcatgtttt ctgcggccaa ttttatctgc agcgttcaca gttctacgat
    45421 agcgggaaac atccaacaag tctgaaaaat catcaatcat atcatctgag cctaagatat
    45481 catctaaatt caactcgtca tcgatttcaa ctgaatcagc gagaaactct ccatccggtt
    45541 cgaattcttt ctccaataaa ccaagagtgt catacttctt aaccttctca gacctttcgg
    45601 gttttcctcg aaatccgcta agacgagccc acaatgcgcg ttcactagac cattcaggag
    45661 ttttttctgg ctctcgtccg ttttcattca caaagtctat aatctcctga aatttagcaa
    45721 cttctgggtc ttgttttaca actttctttt tttcaacttg cttggttaaa tcatcaaaag
    45781 cgtcatcttc aaagatgcta tctaaactat tcgccatctt ctttcgctgc ctctctttca
    45841 cgttttatct cagcaagtcg tagcaaagca aaggccaagt atttttcacc atcatcttta
    45901 ctatttcgat caggatttcg accattttct gcacggaact tcttagcagc ttggtaggcc
    45961 gcaaccaatt gttcgtcagt aaaatcatac ttatttgatg acaaataatc ctgaataaac
    46021 cgaagagtcg gtgcatcaat atcacgacta atcacttcat acgcacgttg gaacggattc
    46081 acttgatcga ttaaatcaat attcaattca tcaatgttaa tgaaacgatc ggccatctta
    46141 ataatttcgc ggctaccatc ttctgaagtt gttgtccgtc cagccgcaat atttatatcg
    46201 gcaactgtat gttgacgaac ggtttctaac tcttcgttcg tcaaatcagg ataacgatca
    46261 atgatgacac gtggaatcaa cgtcttatta atcatcttcg catcagcacc tgaaacaatc
    46321 gcattttgaa tatcttggct ctgtaaaatg ctagatttca aatcttccat atcattttcg
    46381 atgattttct ttgtacgttc cgtcgtatca gcaggtttca gtcctctgat tgaaagatca
    46441 cctggtttgt ctggcttacc atcttttggt ttaaacttgt aaactggcgc caacacttgt
    46501 tccataagta gagatgcaga aattgccttc ataatactgt taaccgcgaa taatacttcg
    46561 tcatcctgtg catctggctg cgcaataaga tttgtaaact gtgcatgagt cttattgggg
    46621 ctgtcacgag ttacgcgacc aatgatttga atcacttcag ttaatgagtt acgataaccg
    46681 attgtcagtg catattccgc aaatggccaa tcgaagcctt cttttgccat tccaagtgca
    46741 atgatgatat caagcttatc aagatcttta atgtcatccg taatacttgc tagatatgct
    46801 gagacttttt cacgtccttc ttcattcaca aggtcggcca ctaatagttt tttaccatct
    46861 ggacgttcga tttcataaat accatcctcg ttctgtgtga tattaccacc gtccgtcaat
    46921 aaatcaagta tttcatcaac ttccgcgtat ttatctttag tagactcacc agaattaaca
    46981 ttaggaatat ggataattga ttttttgttg acatcaatta ctttatccaa agcatctgta
    47041 tattttccac gatagaaact ataccccatt gcaaaggatt tcagatattt atatccttcc
    47101 agttgctcat aataagtgta agtgacctta tcaaatttat cttcgtcggc tggttccaga
    47161 atctgaacag agtcgccacg gaaatatgaa ccagtcattg ctaagatatg cgcattagag
    47221 tttgacatga tgctacgtaa ggcattacct aacacagatt tatcatcacg gctcacatga
    47281 tggaactcat caatcgctaa tagcgtattg tcaaataatg aatcgtccaa ctcttcaaaa
    47341 gcaaatcgca acgtagcatg tgtggcaatc aggatattat cacttgggtc agtcgagttc
    47401 atgaatttaa caaagtgttg aacctttgat ttttcaccgc ccccaatagt taagttgtca
    47461 cttggcttta cttcccaatc ccaataaaaa ccaaacttcg ataattcagt atctttaaat
    47521 gatttaccaa ttgagcgttc aggaactgca ataaccactt ttttaattcc ttgattatgg
    47581 agtttatcca agccaacaaa catcaatgca cgtgattttc ctgaagctgg tggtgatttt
    47641 accaacaaat actgtgacgc acgcttttca taaacacgtt cttgcatttc acgcataccg
    47701 aggtcatttg ttgtttttga ttgattttta tcaaaattaa tttcaaataa attactagtc
    47761 attgattgtc atctcctgat agagttttag taatgttcca agtcgatctt cgtcagattc
    47821 aaacgggcgt tgctgataag ctctatcaac aatcccatcc agttcctggt gtttcttacg
    47881 taatgattct ggcatactgt ctttattata caaatcggct aacgtgccgc cttcgtattc
    47941 tcgtaaatca agaatttctg tcatcacgcg agtcatttca tttttacgct gggcggataa
    48001 atcctgaatc ggaaaagtat tatagactaa cccggctgaa taacggtacc gagtttctaa
    48061 tttcccaccg acagagcgca gccacaccat atgcattcgg gattctaaca gaccaagcag
    48121 ccacattgga gcgttataga ttgccatagc cgaatctgaa attatagtat cgctcccaac
    48181 tatacccata ggaacataat ctcttttttc tgatgacaca cgaggaatta gaatactatt
    48241 ctcactggtt tcatcataac gaacttcacc aaatgaccat ggcttctcag ctagtgctcg
    48301 agtttctttt ctcttgcttt tttccctata actttttaca ttttttattg tttctttaat
    48361 aagagaattt tgtttgtatc tagttgcatc ttcctcggtc aaccataaag tatatcttaa
    48421 gtctcca
//
LOCUS       DAYYAJ010000006        45785 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000006 DAYYAJ010000000
VERSION     DAYYAJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.006"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..2293
                     /locus_tag="ACHATZ_000317"
     CDS             128..2293
                     /locus_tag="ACHATZ_000317"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8402878.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2597..3430
                     /locus_tag="ACHATZ_000318"
     CDS             2597..3430
                     /locus_tag="ACHATZ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8402879.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3532..5232)
                     /locus_tag="ACHATZ_000319"
     CDS             complement(3532..5232)
                     /locus_tag="ACHATZ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402880.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            5739..6899
                     /locus_tag="ACHATZ_000320"
     CDS             5739..6899
                     /locus_tag="ACHATZ_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8402881.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6915..7358
                     /locus_tag="ACHATZ_000321"
     CDS             6915..7358
                     /locus_tag="ACHATZ_000321"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8402882.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(7841..8371)
                     /locus_tag="ACHATZ_000322"
     CDS             complement(7841..8371)
                     /locus_tag="ACHATZ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8402883.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            8476..9027
                     /locus_tag="ACHATZ_000323"
     CDS             8476..9027
                     /locus_tag="ACHATZ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8402884.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(9180..9653)
                     /locus_tag="ACHATZ_000324"
     CDS             complement(9180..9653)
                     /locus_tag="ACHATZ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8402885.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            9791..11263
                     /locus_tag="ACHATZ_000325"
     CDS             9791..11263
                     /locus_tag="ACHATZ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8402886.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            11260..11970
                     /locus_tag="ACHATZ_000326"
     CDS             11260..11970
                     /locus_tag="ACHATZ_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8402887.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            12322..12606
                     /locus_tag="ACHATZ_000327"
     CDS             12322..12606
                     /locus_tag="ACHATZ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8402888.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(12923..13306)
                     /locus_tag="ACHATZ_000328"
     CDS             complement(12923..13306)
                     /locus_tag="ACHATZ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8402889.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(13308..13892)
                     /locus_tag="ACHATZ_000329"
     CDS             complement(13308..13892)
                     /locus_tag="ACHATZ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8402890.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(13939..15414)
                     /locus_tag="ACHATZ_000330"
     CDS             complement(13939..15414)
                     /locus_tag="ACHATZ_000330"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8402891.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            15595..15906
                     /locus_tag="ACHATZ_000331"
     CDS             15595..15906
                     /locus_tag="ACHATZ_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402892.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(16021..18357)
                     /locus_tag="ACHATZ_000332"
     CDS             complement(16021..18357)
                     /locus_tag="ACHATZ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8402893.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            18499..19167
                     /locus_tag="ACHATZ_000333"
     CDS             18499..19167
                     /locus_tag="ACHATZ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8402894.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            19170..20213
                     /locus_tag="ACHATZ_000334"
     CDS             19170..20213
                     /locus_tag="ACHATZ_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8402895.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(20450..21331)
                     /locus_tag="ACHATZ_000335"
     CDS             complement(20450..21331)
                     /locus_tag="ACHATZ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8402896.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            21490..22218
                     /locus_tag="ACHATZ_000336"
     CDS             21490..22218
                     /locus_tag="ACHATZ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8402897.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            22374..22691
                     /locus_tag="ACHATZ_000337"
     CDS             22374..22691
                     /locus_tag="ACHATZ_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8402898.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            22684..23292
                     /locus_tag="ACHATZ_000338"
     CDS             22684..23292
                     /locus_tag="ACHATZ_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8402899.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            23347..24138
                     /locus_tag="ACHATZ_000339"
     CDS             23347..24138
                     /locus_tag="ACHATZ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8402900.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            24276..24764
                     /locus_tag="ACHATZ_000340"
     CDS             24276..24764
                     /locus_tag="ACHATZ_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8402901.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            24815..25687
                     /locus_tag="ACHATZ_000341"
     CDS             24815..25687
                     /locus_tag="ACHATZ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8402902.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            25863..26225
                     /locus_tag="ACHATZ_000342"
     CDS             25863..26225
                     /locus_tag="ACHATZ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8402903.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            26234..26986
                     /locus_tag="ACHATZ_000343"
     CDS             26234..26986
                     /locus_tag="ACHATZ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402904.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            27000..28802
                     /locus_tag="ACHATZ_000344"
     CDS             27000..28802
                     /locus_tag="ACHATZ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402905.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            29079..30119
                     /locus_tag="ACHATZ_000345"
     CDS             29079..30119
                     /locus_tag="ACHATZ_000345"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8402906.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(30171..32315)
                     /locus_tag="ACHATZ_000346"
     CDS             complement(30171..32315)
                     /locus_tag="ACHATZ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8402907.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(32672..34045)
                     /locus_tag="ACHATZ_000347"
     CDS             complement(32672..34045)
                     /locus_tag="ACHATZ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8402908.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(34045..34440)
                     /gene="rbsD"
                     /locus_tag="ACHATZ_000348"
     CDS             complement(34045..34440)
                     /gene="rbsD"
                     /locus_tag="ACHATZ_000348"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8402909.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(34468..35385)
                     /gene="rbsK"
                     /locus_tag="ACHATZ_000349"
     CDS             complement(34468..35385)
                     /gene="rbsK"
                     /locus_tag="ACHATZ_000349"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8402910.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(35493..36506)
                     /locus_tag="ACHATZ_000350"
     CDS             complement(35493..36506)
                     /locus_tag="ACHATZ_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8402911.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(36587..37186)
                     /locus_tag="ACHATZ_000351"
     CDS             complement(36587..37186)
                     /locus_tag="ACHATZ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8402912.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            37372..38073
                     /locus_tag="ACHATZ_000352"
     CDS             37372..38073
                     /locus_tag="ACHATZ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402913.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            38083..40794
                     /locus_tag="ACHATZ_000353"
     CDS             38083..40794
                     /locus_tag="ACHATZ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8402914.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            41245..42873
                     /gene="treC"
                     /locus_tag="ACHATZ_000354"
     CDS             41245..42873
                     /gene="treC"
                     /locus_tag="ACHATZ_000354"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8402915.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(42933..44114)
                     /locus_tag="ACHATZ_000355"
     CDS             complement(42933..44114)
                     /locus_tag="ACHATZ_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8402916.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            44366..>45785
                     /locus_tag="ACHATZ_000356"
     CDS             44366..>45785
                     /locus_tag="ACHATZ_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8402917.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT        14631 a         8113 c         9341 g        13700 t
ORIGIN      
        1 gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa aataaagcat
       61 ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg taaaggagct
      121 aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa aggctttaat
      181 ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga aatcaattcc
      241 cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg aaaatattgg
      301 ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag gaagttgggc
      361 aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa tcaatcggat
      421 cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg catttaccca
      481 cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat tggaagcacc
      541 agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg caatggctta
      601 taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca atggaaagcg
      661 ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc ttgctgtggt
      721 gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag aatcacaaga
      781 aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac tattgccatt
      841 tggattgcat catatgctga cgattcctat caactataca cagcttggcg gatcgtatga
      901 gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt tatggttggc
      961 ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat atgagtttgt
     1021 gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt cttcaggtat
     1081 cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga aagcaagata
     1141 taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa cagaaccatt
     1201 ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag tgatccaagg
     1261 ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg ggaatatcga
     1321 attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc ttatcaactt
     1381 cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt tcttgatcaa
     1441 aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca gtgaagaaac
     1501 cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga tcattcattt
     1561 gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt tacgcgtaag
     1621 tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag gggcgatggg
     1681 attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag atgtcttgaa
     1741 atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag aaatggtttc
     1801 tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag aactagtttc
     1861 cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat tctcacaaaa
     1921 aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg cgccgatcgc
     1981 tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa cgaaagatgg
     2041 tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc cagcttttga
     2101 gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg agatggatct
     2161 tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta caaacgataa
     2221 agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag tgatcggctc
     2281 cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat aagaacagcc
     2341 aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag aagcagcagt
     2401 ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt gataaaaaca
     2461 gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta gcaggagaac
     2521 actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt tcgaaatgaa
     2581 gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc ttgagccaga
     2641 accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt atgaagtgat
     2701 tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg cgaagctaat
     2761 gaagttctgt ccggtaaaga atcaagtacc tatccgtaaa gataattttg cctaccgttt
     2821 agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa tgagccacat
     2881 cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat tggaaagtca
     2941 ggtccttacc gtctcccaat ttcaaaagaa agaagattat cagacacgca agatcttgat
     3001 tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact tttcttggtg
     3061 gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact actttttgga
     3121 aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact ctcaaggtta
     3181 ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg cgaatgaaaa
     3241 aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata cagaaaaatt
     3301 gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc aacggatttt
     3361 tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg aattagatgt
     3421 gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa actctccaaa
     3481 cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg cctaagcacg
     3541 taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc caatcaaagt
     3601 aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga gctcgttgat
     3661 tcctttttgc tgcttcacga ttggggtccc gatatctgtt acttcataat cagtcaatgt
     3721 ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat gaacagtttt
     3781 gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt ttataaaagt
     3841 tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt ttgtctcctt
     3901 atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg tagacatatc
     3961 tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag tatgtcgact
     4021 gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga tcgtctgtgc
     4081 tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt cagaccactg
     4141 gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga aaaatcgaaa
     4201 tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg tccatggaat
     4261 caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt tttggaataa
     4321 ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca atataggaaa
     4381 ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt gactcatttc
     4441 tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta atcctgccca
     4501 aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa gcagtgcttt
     4561 tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca tcaaagctaa
     4621 catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag cgactttgat
     4681 aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata aaggataaaa
     4741 acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag aatacgtact
     4801 catgaataaa acggctactg gtacacttac ccaatttcga acattgacat atctcagtaa
     4861 aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa tctgataatg
     4921 aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa atgggccgta
     4981 aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa aataatggaa
     5041 atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga atggggcatc
     5101 agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca aagcaatgaa
     5161 aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc aaactttaga
     5221 taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt catgaaaata
     5281 tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga tgcgacataa
     5341 gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa acaaagtagc
     5401 tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa taagctcact
     5461 agcctagaaa atatgagaag ctattttctg actagcgctc ttattactca aagcataacg
     5521 cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
     5581 caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac ttgatgcagg
     5641 acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa cccatataat
     5701 aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt aagcaaaaac
     5761 agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta gggttactga
     5821 tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa aaacgtatcg
     5881 aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa atggaggaga
     5941 aaaaccaacg attaactgac caaggattca atcctggtac tgatcctttc aatgaaaaga
     6001 acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga agtatcaata
     6061 tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg atccttgaaa
     6121 atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg acgcattcag
     6181 tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta gatcgtttag
     6241 aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat caagttaaaa
     6301 atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa ggagaagact
     6361 tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg ctagtgacag
     6421 gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga aatcaggaaa
     6481 gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc atactgttag
     6541 tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt gatttttcat
     6601 ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt ttgaaacata
     6661 agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac catcatggga
     6721 aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct gatgatccta
     6781 aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac ttttttgagg
     6841 ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc aatgaataag
     6901 taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa ctggaatctg
     6961 ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca tcaacttatt
     7021 gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt tacttagcag
     7081 aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc gcaggaatct
     7141 ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa aaaataaaag
     7201 aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct attcatttct
     7261 atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta aatgaggagg
     7321 aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa cgaaataatg
     7381 ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta atttaactgt
     7441 gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga ccctcggtat
     7501 tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc taatactcag
     7561 gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg cttctcggta
     7621 ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt cttattactc
     7681 aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc tgaccctcgg
     7741 tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt ctctaatact
     7801 caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact cgtttataat
     7861 tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat ggttcttcgc
     7921 taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta gcaaattcag
     7981 gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt tctgcgatat
     8041 gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta gggaaaaaga
     8101 tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct gtccctgttg
     8161 gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca gcttcttggt
     8221 cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca gtatggtcat
     8281 ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag tgagttccaa
     8341 taaaatcttc caattcttta aattctttca tgtttaagca tctccttata aattaaaaca
     8401 tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta aagatttgtc
     8461 aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt agggttatta
     8521 agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga aataggagaa
     8581 ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga agagcaagga
     8641 atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt ttatgaatta
     8701 acagaagaag ggaaggaaca attggcagag tggattcata cacccaccaa tgaactaccg
     8761 gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa tgatccggca
     8821 ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact tcatctatta
     8881 gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta cggacactac
     8941 ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct aacagatgta
     9001 ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga gaattccttt
     9061 ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca ggcctttttt
     9121 caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat tagtggcgtt
     9181 tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag gagcttgtaa
     9241 gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc ttctttgaag
     9301 ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt gattgctttt
     9361 gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt ttctgcagct
     9421 tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt tatgactgta
     9481 ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat gaatcccttt
     9541 tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa ttcactgatg
     9601 ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac catttttgtc
     9661 actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt gtcaagtagg
     9721 ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa attcagaaag
     9781 aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat ataatcggac
     9841 acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc agaccttatt
     9901 gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg atgtgcaatg
     9961 gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag catggctgat
    10021 caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt tgatcggaac
    10081 gattacttgc tttacagcac ctaattttag tacattacta atcggtcgtt tgatccaagc
    10141 ttgcggtgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta ttttcccgcc
    10201 agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg ctcctgcttt
    10261 aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact tgtttggtat
    10321 ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga aaagtgttat
    10381 tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga ttggattcgg
    10441 tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct tgaaagtaat
    10501 tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc aattacattt
    10561 ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga ttgcagctgt
    10621 tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac cattgtatat
    10681 tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc cgggtgcatt
    10741 gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg gcgcgaaacg
    10801 tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg ctttcttgac
    10861 aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt ttgggatctc
    10921 catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata acttgatcag
    10981 tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg gtacagctat
    11041 tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat ctgtattgga
    11101 taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag gttaccatgc
    11161 cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct ttttattgaa
    11221 gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg agttttgatt
    11281 gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg gcaaaccttt
    11341 ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt cgctaatttg
    11401 tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat tgtttccagt
    11461 gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg aaaagaaaaa
    11521 gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg tacggataac
    11581 gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa aactacttat
    11641 tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa caatcacgaa
    11701 tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt aagtacggac
    11761 caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga aagtgaagca
    11821 aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa aatgagcaaa
    11881 gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca agcattcgca
    11941 aaattagtga aagaagcaaa aggcgaataa tattttgaac accatgtagg aggttgtgaa
    12001 aaagctgctc tgcatcaagc agtaagaaca atcagataaa aatagcactc catatttttc
    12061 atctgattgg gcttagtgcc gtagatgcaa gcttttgaac accgtttaag gaagaggttg
    12121 tgagcctgcc gtttaactct gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt
    12181 tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt attcgaaaaa
    12241 aggaactgcg acaagacttt tgtcacagtt ccttcttttt tttgctatgc tatactagat
    12301 gtaaaaaata ggagaaataa aatgagggaa atttattctt ttgaatcaaa aatctatgct
    12361 tccgaagtcg gcaagggcgg ggcatatatt atttttccgt atgatataag aaaagaattc
    12421 ggtgctggaa gagttaaagt ccaagcaact tttgacaatg tgccgtatga aggcagtatc
    12481 gtgaatatgg gcgtgaaaaa tccagatggt tcagtctgct acatccttgg actacgaaaa
    12541 gatattcgta aaaacatcgg aaaagaaatc ggtgatattg tagcagttac agtgaagcaa
    12601 aaataaaaga ggttgtgaca aaagacgtgt cacaacctct tgataaacgg tgttcaaaag
    12661 ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaacccatg tttacaaaat
    12721 ttttcttact actcgaaacg aagcggcttt atcacaacct cttgataaac ggcgttcaat
    12781 cagctgtccc tcggtattag ctcaaaaacg gcaaaatatg agaagctatt tttccatttt
    12841 tttctctcat actcaggtca taacgctgat ttcacaacct cttctttcat aatggttttt
    12901 caccatcttt ataataaact ggttatttga tatggaaatc tattttttcc aaccaatcta
    12961 aaatcttgta acctaactct tctgacatcg gaaaagaggc gttgatttgt gcattggaga
    13021 tatggtcaac atggataccg gaagtgatcg tacaacttcc cggaacggca gttttgatga
    13081 tcacagctat tttttcggct aagacatcgt ctttatggaa ccgaccatcg tggctaggga
    13141 aacgtacagt ttgcatctct gtatcacgag aaaatgtagt gactgttccg atatgtggtg
    13201 tatctccgcc tgttaaagtg atcaataagt ctttaccgat tactttaaca gcagcttgga
    13261 tcgtgaaacc tagtttggtt acttcaacta tcgtttcttt ttccatatta atcaccctgc
    13321 caacgttttg aatgctcatt tacaatttca aattgatcca aaagtttcat tgtatggtga
    13381 ttaacaagat cttggatcgt ttgaggctga ttataaaaag caggaattgg tggtatgatt
    13441 tgcacaccta gtcgtgctaa tttagtcaag ttttccaagt gaatggcatt taatggcgtt
    13501 tcacgaggaa caatgactaa ctttcgttgc tccttcattg ttacatcagc agcacgtgca
    13561 atcaggttgt cgcctaaacc aacagagata cctgcgactg ttttcatact tgccggtacg
    13621 atgatcattc catcggtcaa aaatgatccg ctggcgacag cagcacctaa atctttgttt
    13681 gaataatggt aatcaaacag gctttcgaat tcagttagtg ataagtcgga ttccagcttt
    13741 aagttctgtt ttgcccatgg actgatgatt ccgtgtgttt ctatcatcgg atattctttt
    13801 agtttcttca ctaggttgat ggcataaatg gtgcctgaag cgccacttac accaatcacg
    13861 atttttttct tccgttgtgc caattgttcc attttccgca ctccttttta tgatgtcaaa
    13921 acagttgcag atcattcatt atttcaaata tttttcccaa tcttctactt cttggaattt
    13981 tgcacgtaca aaatgttctt tcatatcgaa aggaactgta ccgtcaaaga tagtttttgc
    14041 acttatccca cggaaacgaa tagagccagg attatattct ggtaattctg atgggtccaa
    14101 tggatggttc ctcattcctg ggatcacgat gatatcttta tctccttgga aacgagtgtt
    14161 gaaagtccaa actacatcgt tcatgtcgaa aatatcaaca tcttcatcga ctaaaatgac
    14221 tgtcttcaat tctttgaagg ctgataaagc aagaatagct gcttgacgct gaataccttc
    14281 atccgcttcg ttttctttac ggatttgcat gatggtcatc aatttaccac cacctgcagg
    14341 aggattgtag acatttacta cttttcctgg aatagcttta tcgaccaagg caagaatact
    14401 tgcttctgta ggaataccgg ccatagatac gtgttcttca ctgggaccaa tcgttgtttg
    14461 catgattgga ttgtcttttc ggtgagtaac tgctttgact ttcactactt gtacagctgg
    14521 attagcatcc ccagtatagc caggaaattc tggcattgct tttcctgagt tcgtgttgat
    14581 atcttcttgc attgtttcat ttggcatgat ttctgcttca atgatgaatt cagcacgagc
    14641 aatacccact tcatcgactg ctataccatc aacgatttga ataggttcgt tacgcaaagc
    14701 tcctgcacac catagttcat tgtagccaag tggagtggta ggtggttcga aagtcgtgcc
    14761 aatggcaatt gctggatcta aaccaatatt gatagtgata ggcattggtt tattcaaggc
    14821 ttcgaattgt ttttggaagt gaccaatgtg gcgtccgcca ggcatgatgt acataccgat
    14881 cgtatcttta tcttctagaa ccatacgatg gatcgttaca tcgctcattg ttttttctgg
    14941 atctgatcct aagactacac ccattgtgat gtatggacca gcatcttcag gagtatttgt
    15001 aggtgcaggc aaaatgttgc gaatatcgaa gtctgcatcg gtagctagat gaacgacttc
    15061 ttgtgctgga gcgtctgctt tgtctacttt cacaggtttg attggatttt ctacagcgtc
    15121 tttcaagaaa cgaccaagtg ttcgataatc atggtgcaaa atttcaccta cacgtttacg
    15181 actagccatt gttccgatgt tcacacgtgt gccggggaaa cctttgatat tgttgaaagt
    15241 catagctggt ccttcttgag tcggacgttt tactgtaccg ccagcaccga tataacggta
    15301 aacaccagat aattctgctt caggatcgat ttcaatatct gtttcgtgat attgacctgg
    15361 aagtgttttt agttcttcga tgacttttct taaatcatac ggttgatctg tcatgagaga
    15421 ttcctcctta gttgtcttta cctatctata gtaaaaggtg aaaaagagga aaacaattca
    15481 acttgttcgg taaagtattc tacaatggaa tggatgcgtt tttttgataa tagaataaac
    15541 tatgatatac ttatcactaa aagaaagcgc atacttagtg ttggaggaag gaaagtgaaa
    15601 aaaatggagg agtcatttcc taaagcagta aaagtggaaa atatagcgaa tattttgaaa
    15661 gttacatttg aaaacggaga agtaaaatac gtgaaatctc attggacaga agagataacc
    15721 gatgccttgc aatttggaaa aaaaggaaga ggaaaaagaa aaaatcttct cgcactttct
    15781 cgtaatatgt ggattgggac tgaagtaacg attgaagcag atggaacagt attcataaat
    15841 ggaaaagaca ggtatacgcc agaagagttg tggtacaaag gaaagaaaag tataccagaa
    15901 ttataaaaaa taattaaaaa aaacagcgcg aaacaaggaa catattactt cctcgtttct
    15961 tgctgttttc tactatttca gcgctcttat gactcaaatc taaatgtggt tgtcacaacc
    16021 ttattctgca cgcaaagcga tagcggcgtc ttttttcgct gccattcgtg caggaagatg
    16081 accgccaagc atcgtcaata tagtagagac gatcactaga ataatcgcat ggacaggatt
    16141 tagttgagca acatttttta gatccgtcat gttgtataag atgctattga tagggaacgt
    16201 agctagccat gcaataaaca cacctaatat cccagaagaa atacctaaaa tacacgtttc
    16261 tgcgtcaaat acgcgagtga tgtctttttt acgtgcacct aaagctttta gtacaccgat
    16321 ttcttttgtc cgttcaatca cagatgtata agtaatgata ctgatcatga tcatacttgt
    16381 tactaaagag atccctgcaa aagcaataag gacataagtg atagcatcca tcaatccgcc
    16441 agtcagttca gtcatcgttc cagctaaatc agtataaatg attttatctt catctgattt
    16501 gcctttattg taatcatcta gataatccaa gatcttttct ttgtcttcaa aattgttcgg
    16561 ataaatcatg atacttgaag gcaagctgtc tccacctaaa taactaagca atgtttgttt
    16621 ggcattttcg tcaacttttt cagtagttaa gacatttaca ccactgtctc tttgagcttt
    16681 gacgatttct gattctttgt tttcgttgac gatttgagtc gttaattgat cgctataagc
    16741 gattcctgga gataagagat tcattgtaga agaagatttc acgcgtagga tccccgagat
    16801 cttcaattca tcagaagcat tctgatacat cgcatcataa tctgtattag ggataaaatt
    16861 cccagttgga agttttgtat agtaagtatt gttgtttact aatttgaatg ttgtaccaac
    16921 gatatcatca aaatcaagtt tttcgtcttc tttcacatca aatccaagat tcttcaatgc
    16981 gttgatatta gtattattat tgccgtctac gatcagcacg acatctgttg ctgaggctgg
    17041 ataacttccg gcaagtaaag aataattgtc ttttaaaaag ttttctttgc tagtgtcaag
    17101 ctgtgtaggg aaacttgaca ctcccacacc tgtcattgct gacatcgtac tagataaaga
    17161 aagagattcc gtatcaggat tttgattcga gaagctgaca ggctgtactt tgccatttac
    17221 atcacgtaaa agattgattc ctgtagtacg ggtaaatccg atattattac ttaagttagg
    17281 atcgatgtct gttacgtaat cgacgtatgt ttgatcaatc ttattcgtgt gctgtgcttt
    17341 atcttcttcg ctgacttttg cagtaatcgt tttgctgtct ggataatccg ctttgcttgc
    17401 acccaatccg gcatcgtcac gagtctggtt agtcgttacc ttagaaatag taatcggaaa
    17461 tttcgccatc gtttcagctt gcgtgttatc gatttgtttt tggaatccag aagaaaggga
    17521 cagaacgatc gcgattccga tgatcccaat actggaagca aaggaggtca agaaagtacg
    17581 tccttttttt gttcggatat tgttgaagga aagctttaac gctgtccaaa aactcatctt
    17641 tgttctgcgt aagttaaatt gatcatcttt tggtcgttcg atgtgaggat gggaatctgt
    17701 taagatctta ccatcagaaa attcaatgat tcgatctgcg tattgatggg ccagttcagg
    17761 attatgtgtg accatgatca ctaatttttc tttcgataac tcttggatca attccatgat
    17821 ctggatactt gtttctgtat ctaacgctcc ggttggttca tcgcacaaaa ggatatctgg
    17881 gtcattggct agtgctcgag cgatggccac tcgctgcatt tgtccaccag ataactgatt
    17941 tggcttttta tgcatatgat ttgtcaagcc gactcggtgc aatgcatctt ctgcccgttt
    18001 tcgtttttcg tctttagaaa caccacttaa tgtcatccca agttcaacgt tttcaataat
    18061 gcctagatga ccaatcaagt tgtaactttg aaaaatgaaa ccgatagagt tgtttcggta
    18121 ggcatcccag tcactgtcct taaagtcttt agtagacttt ccgttgatta ccatatcacc
    18181 ggaatcataa ttatctagtc caccaatcac attcagcata gtcgttttac cagaaccact
    18241 aggacctaaa atcgctacga attctttttg tctgaaagcg acagacacac catccaatgc
    18301 tttggtggtg gtttcgccga ctttataata tttttttata tcttttaatt gaagcataat
    18361 cacccttctc ccgtattgaa atattcggta tactaataag tatatcagaa caatgatcgt
    18421 agtatacgaa ataaatgtta actgaagatg aactacaaga aaaaatctag aaatttttca
    18481 aaaaacagag ggagatcaat gaatcgtatt ttagtagtag aagatgatcc gcaattacag
    18541 ttgctttacc gttctgtgtt ggaacgagcg ggcttttcag tcatcaccgc attgaatgga
    18601 gaggatgcgc tgaaaaaact agaagcgaca cgtatagaat taatcattac agatatcatg
    18661 atgccgaaaa tggatggcta tgacttgcta gactcattac gtagcaccag atttgacaca
    18721 cctgttctaa tgattacggc aaaggccgat tttgaggata agaaacgcgg gttcaaatta
    18781 ggagcagatg attatatgac aaaacccatc gatgtcaatg agatgatcct acgtgtggaa
    18841 gcattgcttc gacgttcaaa gatcaattac agcagtagct tgataatcgg acaaacaaaa
    18901 ttagagctag atgcttatac agtgttacaa gatcagcatt cgttgattct gcctcaaaaa
    18961 gaatttctgc ttctgtataa attactgtct tatcccaaca agatcttcac tcgccaacag
    19021 ttgatggatg atatttgggg attagatacg aatacagaag aacgaacgat cgatgtacat
    19081 atcaaacgtt taaggacccg ttttgaaaag acggaagatt ttcaaatcat cacagtacgg
    19141 ggactaggct ataaggcggt tatttaaaga tgaaaaaaaa gctattttca caattatgga
    19201 tctattttgc tagtattgtc ttcatttcga tcttagtgac actgctgtgt tttctcggtt
    19261 ttattttctt attgtctcga cattctatct cgcctgctga atcggctgga aaaccaacgt
    19321 tatacccgtt attcgtcttt gcggggttca gtatggtcgt gggtacagga atatcgattt
    19381 ttgttgggcg gcgtatcctg catccaatca gtgcgctagg gacaaatatg agtctggtag
    19441 caacgggaga tttttcgatt cgaatggatg aacagcagaa agtagcagaa gtacagcaac
    19501 tgtataaaga tttcaacgtt atggtccaag aactcaatag tatcgagaca ctgaggaatg
    19561 actttgtgtc cagtgtatcc catgaattca aaacaccact agcaacaatc caaggatatg
    19621 ttcaattgtt acaagcccca aatctctctg acgaagaacg ccaaatattt ttatatcgga
    19681 ttatcgaaag tatcacgcag ctttcacaat taacagagaa catcttaaaa ttgaacaaac
    19741 tggaaaacca gcgtatccaa ctggaaaaga aagagtatcg attagatgaa caaatcagag
    19801 aagtaatcgt tttttttcag cctaaatggg agaaagaaca gctagaattg gatatcgaac
    19861 ttgctgcagt aaattacaca ggcaatgaag agtttttgta ccaagtgtgg ctgaatatca
    19921 tggataacgc cataaagtac aatcaaatca atggacagat tcatatcaaa ctatttgaga
    19981 cagccacaga gattgtactg gaagtgaccg attcaggtgt aggaatgaat gaagagacca
    20041 gagatcgaat gtttgaaaaa ttttatcaag gagatacaag tcgtcaaatt tctgggaatg
    20101 ggctaggact ttcactggtt aaaaaaattc tagaactgca tgatggacgt attgattata
    20161 gcagtatcga aggagtagga acgacagcaa tgatacggtt aagtaaacaa taggaaaaag
    20221 aataaaaccc ctaaaaattc tattgaaggt ttttaggggt ttttagtcgt ttttttgtga
    20281 ttagggaaat gttcttacgg ttcttttggc ataaaaatcg ctgctttctt attgaactac
    20341 gagaaatgtt agaggtgtca ttgaaacaag gacatgatgc aagaaagaga gcgaaagtca
    20401 tgcgtcgctc tctttttttg ggtggataga tggacagaaa atgtagggct tagttttctt
    20461 cttgatttaa atactcccaa aatgtatctg aagccaaaga atgctcaccg actttacgag
    20521 taaatgcaag ataactctct gttgttggag aaatagggag aatcacagta ttttctttta
    20581 atagattctt gactgttttt tccataacaa tagcaatccc catgtcattt gcaatcatat
    20641 tgatgatcaa gtcgattctt gctcctttat aagagatttt tggttcaaat ccagcttcgc
    20701 gacacagatc tagtacaggt tgatacaagt tcgtagtggg gccaaggagc aaaaagttct
    20761 cctttttcag ttcgcttaga tccaatttct ttttactagc taaaggatga tttttaggta
    20821 gaactgctac aaaatcgtct gtttcagtag gcaaccactc aatcgaactt ggtggggcct
    20881 ggaaacttct agcaaagtgg atcgtgtcac tatgttcatt ggcgtcattg taagttaatt
    20941 cgacactttc cacttctttc aactggactg taaattcagg atggcgtttc aaaaactcgg
    21001 tgattttgga aaaaccttta taattgatca ttgtcggtat cgttaagata tttaatgaaa
    21061 gattgttgtc tttcgtatag gtttcaagca cttcttgcat agcatggtat tgttctacga
    21121 cattttttac atatgggagc aataagcttc cagcttcggt caattcgatt tttctatggg
    21181 cgcgttcgaa taatttgacg tctaattctg tttctaacga aagaatctgt ttagaaatat
    21241 ttccttgggt ggtgaattgt cgatcagcag ttttggtgaa attcattgtt tcggctaaat
    21301 caagaaaaac ttcgaattta tggatatcca tcatattcct cctacttatt ccaataaaga
    21361 atactttaac acaaaaaatg aattggtgta atcttctctg tctgttacaa tctttcttgc
    21421 agcgaaagca ataattacag aaatttttct gatttttttc gcttcgtaga aaaagagagg
    21481 agttttttga tgccaactgg agttttgatc aatgtaggtt ccgttttgtt tggcggacta
    21541 attggcggat tagtaggaaa taaattaagt gaacatttta aagcacagct gacgatggtt
    21601 tttggtgtgt gttcgatggg gatgggaatc tattccattg cgccgatgaa aaatatgccg
    21661 gctgttattt ttgctttagt gataggaaca gcgattggtt tgattgttca tttaggtaat
    21721 gggataaata agggggctgc gctgatgcaa gtacctattt ctaaaatctt ccctagtgaa
    21781 aagcttggta tgacccacga tgaatttatc agcacattag tgacggtcat tgttctattt
    21841 tgtgcaagtg gtacggggat ctatggaagt ttagattctg ggatgacagg agacagtacg
    21901 attttgattt ctaaatctgt ccttgatttt ttcacagcag ctatttttgc gtgtaattta
    21961 gggtatgtcg tttctgtgat agcaattccc caattcatta ttttttatat tttgttctta
    22021 ttggcaaaat ttatttatcc attgactaca ccagacatga tcttagattt taaagcttgc
    22081 ggcggattct tgatggtcgc taccggtttt agaatgatca acgtgaaaat gttcccagta
    22141 gctgacatga tacctgcaat gatcgttatc atgccactga gctggatgtg gacaaattgg
    22201 attatgccac tgttataata aaaatcaaag agaagccatt ttttgctgac taaaaaggat
    22261 caaatctttt agtcagtttt tttgtatttg acaaactgat tttgaaacta tattatatgt
    22321 tgtataatat agattgaaat aaaatattac acttgagaat agaaggtgac gtaatgcaca
    22381 tccagattcc aactgttctt cttgatggta ctgtgctagc agcattaagc aaggaagata
    22441 tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt gaatctacac
    22501 tatatccggt tttgagaaga ttgaaaaaag aaggatatct tgagacctat gatgaacctt
    22561 ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt catttagaag
    22621 aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg gaggagagca
    22681 caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc tgaagacgat
    22741 cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa aacattagat
    22801 cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa aatcagttac
    22861 ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag acaaattcgc
    22921 ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc actagcactg
    22981 gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat ttttagtatc
    23041 tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc aggaatcagt
    23101 gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct gttcgccaca
    23161 ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct tttttatgtc
    23221 atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga tctgcattca
    23281 gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat taccggtatt
    23341 ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg cgctcaaaag
    23401 agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac aaaaaaaatc
    23461 aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt catacgcgga
    23521 ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat aaaggagaaa
    23581 aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat tagtttagaa
    23641 gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc tgaattgaca
    23701 atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa agtgaatact
    23761 aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat aactgcgaaa
    23821 gatgaattag gctcacttcg attagatgag gccaatgtgg ataacttgat cgtcacaggt
    23881 gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa agtcatccta
    23941 aacggtggca atttatcttt gcatgagatg gaaaacagtg gctatactat ccaaacgtct
    24001 ggtgaatctt atattgaaaa agactaccag ccaataacaa atactttgtt agaacaagga
    24061 cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac ggatcaagag
    24121 gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa cagcaaagat
    24181 ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa aataagaaag
    24241 aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa ggtcaaagag
    24301 aaaagttaaa aacagaaatg gaaagattac atacttatat cactcaacta agccaaacct
    24361 tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca gaaggtcgcc
    24421 aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag aaagagctgg
    24481 attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg aagaaggaac
    24541 gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg gatctagagg
    24601 tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa ttggattatt
    24661 taccgcaggc agccggaggg acacaggcaa gcggttggta catcactgaa gacaaagagt
    24721 tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaaa ttaaaaaagt aacattagat
    24781 atttattgaa ggataaagga gcgcaggaag tagcatgaga gaaaaaagag cattttatat
    24841 caatggttat gcagggattg tcggattggt cgtattagct ttagttggat tgtttttctt
    24901 ctatttaggt atgtggcaag cgaaagtttg ggcacttttt ttaagtattt ttctttggtt
    24961 gatcacgtta ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag caaaagtaat
    25021 tttgtttttt ggacaatatt taggtacgat tcgagaaaat ggctttttcc ttacgatacc
    25081 tttggcacaa aaaatgactg tttcgttgaa agttcggaat ttcaatagtt ctgttttaaa
    25141 agtcaatgat ttggatggaa accccatcga aatctcagca gtagtagtct ttaaagtcgt
    25201 tgatacagca aaggcattat tcgatgtagc gtattatcaa gattttgtag aaatccaaag
    25261 tgagacagcc attcggcata tcgctagcca atacccgtat gatacattca atgagaatga
    25321 tttgacgctt cgaggaaaca cgacggcagt gtctgacgag ttgcaaaaag agctgcaaga
    25381 gcgtttagca gtagcaggtg tcgaagtaat cgaaacacgc ttgaaccatt tagcctatgc
    25441 aacagaaatc gctagtgcca tgctccaacg tcaacaagca aaagccattt tgtctgctcg
    25501 acaaaccatt gttgaaggag ctgtcacaat cactcaaatg gccttagagc aaatcgaaga
    25561 tggacaagaa atcaatttca ccgatgatcg gaaagtccaa ttgatcaata atttacttgt
    25621 ctcaatcatt acagacaaag gaacgcaacc tgttatcaat acaggcgaca tgaaagaccg
    25681 taagtgacat actttttgta agataggaag aaggagttta ggacggatta ttgtcttgaa
    25741 ctcttttttt agttgtttta aagaaaatga caaaattgaa agaagaatga tagttgattc
    25801 gaaggcagaa ttgttcgctt ttttctataa tcagtatata aagtaagaga gaaggggaaa
    25861 aagtgaaaaa acttctgatc ggtctacttg ttttcatcgt tgtggtactt gcaggggtga
    25921 aaatcgcaga ttacgtagtt atgggtggag aagaatacta tgtacaaatt acgacagatg
    25981 gtacaaaaac agtttcaaaa tcagactctg gacaagaaac agtcaactat agatatatcc
    26041 tgcctggcta tacgaaagag ggtaaggaaa aagaactgga cttcaaggga caattgactc
    26101 gaccgcttcg taaaggagca tatttaaaaa tcacctggaa caaaaataaa ggtgtcactt
    26161 cgtatgaaga agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa ctaattaaag
    26221 ggtagggaaa gaaatgaaca aaatagtcga agtacagaat gtaacaaaag tatacggaaa
    26281 aaacaatgag aaaaagacgc aagcattaag cggcatcagc ttcgatgtag aaaaaggcga
    26341 atttattggg atcatgggcg cttctggttc tggtaagtcg acacttttga atattttatc
    26401 cacattagat aaaccaacag atgggcatat ccgaatcaat caaaaagatg tgaccacatt
    26461 aaaaggaaat caactagcag attttcgtgc gaatgagatt ggctttattt tccaagattt
    26521 caatttatta gaaagcttga cagcacaaga aaatatcgct gttcctcttt ctttacaagg
    26581 cgtcagacca aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt tatccatctc
    26641 tcatattttg gaaagctatc cttctgagat ttccggtgga caaaaacaac gggtcgccgc
    26701 cgcacgtgca ttgatcacac aaccaacgat tctattaggg gatgagccta caggtgcgct
    26761 ggattccaaa agtgcccggg atcttttaga tacgatggac gaattgaata caaaagatca
    26821 tgtatcgatt cttcttgtga cgcatgatcc gttttctgca agttattgcc aacgtatttt
    26881 attcatcaag gatggcggta tccatcaaga agtgaaacgt ggagatcaaa gccgtgattc
    26941 cttctatcga gaaatactat ctattcttgg aaacttggaa cagtaggggg agattgacta
    27001 tgctttggaa attatcactt actgggatca agagccgttt aagagactac ttggtgttat
    27061 tctcaggatt agttatggca tctgctattt tttacatgtt tgaatcaatg gcaagcaacg
    27121 aagctttttt gaaaagtaat acgatcgttg cggctacagt tatcattttc cgattcggtt
    27181 ctgttttact agggatcatc acattcgttt atattttata tgcaaattcc tttttgatga
    27241 cgatgcggca aaaagactat gcaatgttta tgatgctggg agcaaaaggc agaaaaatcg
    27301 cacagatgat tttttctgaa acattcttag tagggatcgc agctacttta gtgggttcag
    27361 ctgtcggtgt aggattgaca agtatcgtcc accgattatt agtggatcag ttagatctca
    27421 atatttcgca ttttacacct tttagtattc acgggttgtt gatcactgtt ttgttttttg
    27481 ctgttttgtt tttattagct gctgttgtca atgctttttc gattgtcaaa aaaccgattt
    27541 tagcattgat tcgtgcggat cagacaccaa caagaatcaa gcagcataaa ttcttgtttt
    27601 tccttgaagt agtactggga attgcttttc taggctgcgg ttactatatg atgaaacacg
    27661 tggggaccta tcaagtcttt ggtatagggg tagccttagt tacgatcgta ttaggaactt
    27721 atttcatttt ccattctgtg atcattttct ttttatcatt actaaagaaa tcagaacaga
    27781 tttcattgaa aaaattgaac aatttcacat tgtcacagtt aaactttcgt attcgtgaat
    27841 atacacaaat gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc gctttgacgg
    27901 taggattagg ttttaaaaat gaaattgcag acttgacaga aaacaattct ggatatgatc
    27961 tagtgatgaa taatcctcgg gcagaagatc agcaaaaaat caaagaactt tcaccaacac
    28021 tttctgcttc ttatactcaa aaagaagatg cgggaacgat ttactataat gcaagtgaat
    28081 ttgcggctca accattgatc atgataaaac atgaagatgg aacaccgccg aagatcacga
    28141 aagaaaaagt tcctgctaaa aaaatgaatg aactgactgt acaagaggaa cttcgaagct
    28201 atgaattgcc ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca gaatttgatc
    28261 aactgaactt gccacaatcc actttacagc tagttcaggt aaaagacttc caagcaaacc
    28321 tgaaaccaat cgaaacactt gttgctcaaa ataaaatgaa caatccatct ttgcaaaaag
    28381 tagagtacag caaccagaaa tatgaaatgt atgaagtgta taatggtcta ttctctggtt
    28441 ttgaatttat gggattcttc ctaggtttag ctttcttgac catgcttgct agctgtttga
    28501 tgttcaagat cttgtcagga agcaagagtg acattgctcg ttatgaaatg cttgaaaaga
    28561 taggaacacg acgcgggttg cttaaacaat cgatccgtag agaagtaggc gtgttattct
    28621 tagcaccagg aatcttaggt gtagtccatg tcttattcgg attacaaatg tttggtctat
    28681 tcatgcaaaa tccatacaaa gatatttggg ttccgtttac gatattcttc gtattgtatt
    28741 tcatctacta tgtactgacg acatggttat atactggaat cgtattgaaa aagagattgt
    28801 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagct cctcatattt
    28861 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaaaa
    28921 agaggttgtg acaatatttt tgtcacaacc tttttttcac ggtcttttat tagaatatag
    28981 taaatttttt ctcgttactc acggaataag ctgttaagaa aacggtttcc atgtatactg
    29041 gaatcagtgc aaatgaaaga aaagagggtg caaggaacat ggaaatcaat gaacaaaatt
    29101 ttggcgaaaa ctatcacttg attacgatca cgaatcaaaa tgggacatct ttgtctgtca
    29161 gtgatcttgg ggcacgggtt gttcattttc aaacaataat cgataatgaa aaacgagagt
    29221 tgatcttagg ttttgatgaa gcaaaagaat atctagaaaa agacccttat atcggtgctt
    29281 caatcggacg tatggctgga cggattacga atggcacatt caaattagat gatgaaacgt
    29341 tcgaagtaaa aacagacccg gacactggtc attgtttaca tggtggtgca cctagctttg
    29401 aagccaagca gtggcattat acagtcatca atggtgaaaa cgaagcatcg gtcattttct
    29461 atcagactag cccagatatg gaaaacggat ttcctggaac tttagatgta gaagtacggt
    29521 atacgctgac gaacgatgat acatggcgag tgacggtcca agggatcagt gaccgcaaaa
    29581 cagtattcaa tcctacaaat catgtctatt tcaatttaac cggtgatcca gcacagtcaa
    29641 tcgacgaaca tatactttgg ttgaatagcc gattatttgc tacgctcaat aaagatacaa
    29701 cgcctacagg agaagtagtt tcagtaagtg gcacagcatt tgacttccaa cgtcctaaaa
    29761 aattaggtga ggtctttgcc agtgattttc gccaatcaat gatagtgggc ggcatcgatc
    29821 atccattttt gttgaaaaaa caatctggtt tactaaatgc tgcggaattg acaagcccag
    29881 atgaaaaagt atcgatccaa gtaaaaacag atgcttcagg aatcgtgatt tttacagcaa
    29941 attttggaga agatggaccg gaaatgcgcg ggaataaatt agtaaatcat ggcggtatca
    30001 catttgagac tcaagaactg ccaggagcgg agcgacttga aagttttgga gatgtcactc
    30061 tggaacctaa ccaagtacgc gagtttgtaa cggaatacaa aataaatata aagaaataga
    30121 ataaattaaa aagggagtgt gacagtgtca catccccttt ttaatttatt ctatttcttt
    30181 aaaggcagat caagccagaa tgatttgtct aaccttaaca aggttagtac aggaacagtt
    30241 gcagctttaa ctgtacgagt agttgtttct ggtatctgtc ttgccgtaaa attgttccac
    30301 aaatcccaga ttgaagcgac acggaagatg gaagaagcgt tgttgacgcg ttttatcgtt
    30361 gaaaattcta atcctgtgat tggcacgcca tctgttgtcc atttgattgg cacatcgaat
    30421 cgcttagaca tattgaggat ttcagcgttt gcttcttcag ttgttgctgc ttcttttttc
    30481 ggttcatcat attttgtcag gtcgtacgct tcgatgatcg cgttcaattt ttgtgaatat
    30541 tggcgatctg tagcataacg tccttccaaa tatttggttg cttctttgta gtcttttgtt
    30601 tccgacttcc aagttggttt gtagaaatca ggattgccag tcagtccatt tttgatcaag
    30661 tcggcatagt cttccaaact ttccttgtaa gaaggatatt tacggaattg tgcgcggatt
    30721 tggtagcttt gtccggaact atcttgttca agtgtattga aggtcacgct gcttccttta
    30781 tatgatcctt tgataccaaa aaggttatga tttggttcgc ttgataataa gctgtttcca
    30841 gatccacttt caaggatagc ttgagccatc atgactgatg cgtagattcc ttctttttgc
    30901 ccgatttcct gtgcatcatc tgcaatcttt ttcaagaact gatcagttgt ttggttttga
    30961 ctgaaatgga acgagccatc taatccatct aaaatagatg agccgcctgt cagcgaacct
    31021 gaagcagcag gtaaacctgc taagtttact cggacagcaa aatcaggcgt gaaatctgaa
    31081 atagaagtta ctcgaacttt atctcctggt tgaggcgcat ggataaattg tccgttccca
    31141 atatagatac ctacatggtg agtaacaccg gaagaaggtc cccagaagac gaggtcgcca
    31201 ggttgggcct gacttactgg aatctgcgta cctgcatatt gctgttcgcc tgtccagcca
    31261 ccgatataat gaccggttgc tttcatatat acgtaataag tcaaaccaga acagtcaaaa
    31321 gtatttggac cttttgctcc ccatacatat ggttttccaa gatgcttgaa tgcttctcga
    31381 acgattgctt gttggacatt cgttgtattt gtaggcaata cagcatcaat tggtgatttt
    31441 acaacaaaat cagaagaagc agaagatgga ttctctatac cgctttgatt tgctgtattt
    31501 ggacgagatg aagcattgtt cgttccgcca ttcgatgaac cattgtttgg atggtttggt
    31561 tctctctgag tatttccatt gttattcgat ccagatgggt ttgtttgatt cgaagaattc
    31621 ccgctgttcg aattattgtt attattgttt gaattatttg aaccattatt attgttgtta
    31681 ttacccacat ctggtttgtt tggcgtttcc ggatctggtg tactagggac ttcttcacta
    31741 ggatcagttg gtttctctgt tgaagtatcc ggtccagctt taaattctgc agtaatagat
    31801 aaattgttcg tacctactgt aaaggtcttt gcatcataat ttacttcttt ggcaccatta
    31861 acagcgactg aagcaatttg ttcaccttca gctggcgtaa tagtgtaagt taccttcgtt
    31921 tccgctaata aaccttctac cgttccactt tccgtaatat ccaaaggttg ctgttcaggg
    31981 gaatccgctg tcgaagcagt cagttggatc ttaccaatca aagaaggagc aattgtaagg
    32041 gaatatgtct cggctggctt ttcattttct gatggatcag ccggatcaat ttcagttggc
    32101 tcggtcgatc ctgttgttga gttagttgtt ccagttgggt cttctgtaaa agaatccgtt
    32161 gtttcactag tagctgagct ggatgtagtc gtatctgttg tgactgtttg atcagctatt
    32221 tgattatcta cggcaaatac ggtattggtc gacaaaacag acggagcaat cagccaagta
    32281 gcagctagca aacgtatcgt ttttttattt ttcataataa actagtcctt ttcctttcat
    32341 tgattttctg tcttattcag cattcattag acattttttg gaacagataa tcgtgtcgaa
    32401 aagctttttc ttttcataaa tctgtctgtg tattctaaat aaaacaattt gtaaataagc
    32461 aacgagcatt cattcgtttt tctttgacgt tcattttatt gcatagacca cctttgtttg
    32521 gggataaaca agtggggata aaaatggtta tgcatatgta aattatacca aagtttggaa
    32581 agtatcacaa aaatttataa aaataatgat cctaaaaaga aaaagactga aatatgtgat
    32641 aaacatctca tacttcagtc tttttcatta actattcata agacattagg tcttcatcaa
    32701 acccgctaac gagagtagtg atcattgcta cgactagact aacagccagt cctgctaagt
    32761 accagaaaaa agtaccggaa atgaatgcag gcaatgtggt cagactacca aatacaaaag
    32821 cgatcgcttt cacttgggca aatcccatta cagctccacc agcagctgca ccaatgcaag
    32881 cagcaacaaa tggttttttc aatgggatcg caattccgta aacagttggt tcggtcactc
    32941 ccgcaagtaa aatcgaagca aaagcagaac cggagatcga acgggttttt gaacgtttac
    33001 ttcgcagaaa tacacctaaa gctgcaccag ccattccaaa attggctgct cctgtcaacg
    33061 gaacgattcg gtcgaaccca taagcagaga tattactgat catgacaggg tttacagccc
    33121 attggatacc aaatattacc aaaatattcc aaccaccagc gatgattgcg ccactgatcg
    33181 gtccgttaat atccatcatc caagtaattg cggcagctaa accttcgcca aggtaaacac
    33241 ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta
    33301 caaatactaa ttgcatattt tcgcgaatat attttttgag gaatttttca acaaaagaat
    33361 aaaagaaaat acctaaaatc gccggcatga cagtagacgt atactgcatc agtactacag
    33421 gaatccccag aaaatcagta gctgctttgc cactgtctga aagtaaagag gtcagattcg
    33481 gttcaatcag tgctgcagca atgacagccg caatatatcc attcacttta aacgttttgg
    33541 cagcagaaaa agcaagaaag attggcagaa aataaaagac ggcattcgac gcagcagaca
    33601 aaatatgata agttccgctt gtttcactaa gccagccaat cgtagtcgct aaaacaacca
    33661 gaccttttaa tataccagaa ccggcaaaca gcggaatgat cggcgtaaag atttccgata
    33721 tcaaaccaaa tagtttttcg atcggattta gcttttcttt cggagctgaa tgatcgattt
    33781 gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc catgattatt tctgcatatc
    33841 tatcgagtaa aatgaaatac ttaatggaag ggatttgctg ttttaatttt tctgcattgg
    33901 ctactggctg ttttttcaat ttgatacgaa gccgagtcgc acatggaagg acagaatcaa
    33961 tattgtcttt tcctcccaac tgttcaatca gcatatgaat ctgctcggac agtaccatct
    34021 gttcaggatt cattgtcgtt tccattagaa gaccactcca ctcactaata taatattggc
    34081 gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag ttattcaact cttttttcat
    34141 ttcttcatgt gggataaagc taatcggtgt atctggtaac aataatttga ttttttccaa
    34201 tatttccggg ttcattgtct ttatttcttc cgccaattca atcgattgta cctcaagttc
    34261 ttctaaaacg ttttctaaaa cagataaaaa acttggaaga ccgtttgaga cagccaaatc
    34321 tattttttca gtaccaaagg ggacaggcat gcctgcatcc ccgatactta atttatcaaa
    34381 gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg attacttttc cttttttcat
    34441 gtttgtttct tcctctcatt gttaatttta gactgctgat tgttctaaag cttcgcggta
    34501 agggatagaa ggttgcgcac cataagactg cacagcaaca gaagaggcta gatttccata
    34561 aaggatcgct tctttcaagt tactaaggtc tggctgaagt gtgctgctta atgcgccaat
    34621 aaatgtatca cctgcggctg ttgtgtcaac ggcatccact ttaaaagcag ggacgatacc
    34681 gtgttctttt tctgtatgat agaaagcgcc ggcactgcct agtgtaatga ttactgttcc
    34741 aatacctaat tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct
    34801 gattcctgta atgatttctg tttctgtttc atttggtata atgatatctg tcttttcaag
    34861 tagttcagta ggaatcgttt cacgagctgg tgcaggattc aggatcgttg tttttcctgc
    34921 gtcttttgca atagaaaaag ctaggatcgt cgcatctaag ctactttcaa actgagcaat
    34981 aataaagtca ctgtttgtaa tcagttgctt gttttttagg acttcttgtt catggaaagc
    35041 attatttgcc cctgcatgga tcaagatact attttctcct gctgcgtcta ccataatcat
    35101 tgcttgtccg gttgtggcgc cttggatttc agctatccca gatgtatcga tgttttcttt
    35161 tgtcagtaga tctaataact gttgtccttc gctgtcagcc ccaacgcctc caataaaaga
    35221 tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg gcacctttac ctccgccagc
    35281 gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag
    35341 ggtagtgtcc atattaatac ttccaattac tgtaaccgtg ttcattttac aaacctccaa
    35401 ctatctcatt tattttgatg ggaaaattat tttttaaaac gttttagtaa aacgttttaa
    35461 tagttcgata aaaatatagc acaccttgaa agctatttca agggtgcagt tgaaaatctt
    35521 ttttctaatc gtacaggtaa tcttttttct tcccattctt tttctgggaa ttggatccga
    35581 tctaacaata acttggcaga tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc
    35641 agtttcggct tgatatactc gcacatatct atattatcat agccaataat gctgtagtct
    35701 tcagggatgc tttttccagc ttcctctaat ccgcgatata atccaaaagc taattcatca
    35761 tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca
    35821 ccttgttttg agaactgtgt aggtaagagg atcagttggt cgtgagggat ttggtaaaca
    35881 tcaagagcag atttaaaccc ctcaatccga gcgtggacat tttccggtgg attttcagga
    35941 tagacaaggg cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc taggtaacca
    36001 ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt cagatttttt ctgatccaaa
    36061 acaatgaagg gccggccttg ttttttcaaa ttttcgttga ttgcatctgt agagacagca
    36121 gaagtcgcga taatgaatcc atccacacct cgacgtgtga gctcggctag atattcgatt
    36181 tctttttgat gatcagaatc agcattacat aatatcgtga cgaaattctg cttatacaag
    36241 atatcctcaa ttcctcgcat cagtgtactg aagaatggat tcgtgatatc aggaacgagt
    36301 acaccaattg ttttcgtttc cttggtaatc atttgtctag caaaatagtc aggctgatag
    36361 cctaattctt ctttggcagc taaaactttc tgaacagttt tcggactgaa tttctccgta
    36421 tttccattta atatcaagga gacagttgtg attgaaacgc cggaaagttt agcgacttct
    36481 ttgattgtga tctttttttt caccatgttc atactcctat ctaaatcctg tggttatttt
    36541 cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc cagtacttat ttgctttgtt
    36601 ggatagcttc gattatttgt ttcgttgagc gtactcgtgc agttttcgga aatatctttt
    36661 ttatgaacaa ttgatgggat tctttgtcac gatcactgca agcatcttca gcaagcacga
    36721 tatggtaccc gtgttgaaag gcatctaatg cagtagcata tacaccgttt gttgtcgcta
    36781 caccgcttaa aatgatagtt tcgattccac gccggcgaag ttgaagatca agagaagtac
    36841 caaaaaaagc acttggattg tattttgtga tagtcaacat attatctgta tctttcaaag
    36901 aatccttcaa aagtaaatca gtgaattctg gaggtacaga agcagtcatt ccacccatat
    36961 cagtttgttg actcaaataa gggaaagtgg agatgtctac gttcactaaa actttcaagg
    37021 cagcggtacg tttcaatgtt cttactaaca aatcattgtt tgtcagaata gatgctgcag
    37081 tgtgtggaga gagtgtaccg ttatttacga tctctttttg catgtcgatc gcgacaaaag
    37141 cagtttttga taaatcaaaa atcatatttt cctttaaatc attcatgatg ttccttcttt
    37201 caaataacaa tgttttttat gtaagtaaat cataaatcgt ttatttgtca actaaattaa
    37261 aggattcctt tctttagtct agagaaattc agtggtaaaa taaataagtg tgagaaaacg
    37321 cattcgatgc atagatatag cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat
    37381 gtcgaagtaa aaaatgaata caaacgatac aaaatgggag aaacgaccat tacagcaaat
    37441 gacgatattt ctttttcaat tgaaaaaggc gaattagtga ttatacttgg tccaagtgga
    37501 gcaggaaagt caacgattct taatatttta ggtgggatgg atacacctga tgaaggtcag
    37561 atcattattg atgacacaga tattgcgcaa ttttcagata aacagcttac tgcttaccga
    37621 cgaacagatg tagggttcgt tttccaattc tacaatctcg ttcctaatct aacagcaaaa
    37681 gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc ttgatcctgt agaggtattg
    37741 aggcaagtgg gcctagctca tcgattgaat aactttccgt cccaattatc aggaggcgaa
    37801 cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat
    37861 gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac
    37921 gctagcagac aacacggcaa taccgttttg atcattacac ataattctgc tttagcgccc
    37981 atcgctgata gagtcattca cattaatgat gctaaagtaa ggtctgttga attaaatgat
    38041 catccttcat ccattgatga gattgtatgg tagggggatt ttatgaaaac aagaacatat
    38101 cttaaagcag gttttcgcga aattcggcaa tctaaaggac gattcattgc aatcatcctt
    38161 attgtcatgc taggaacact tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa
    38221 aaaacgatgg atcactatgt tggtacagca ggactttctg atttgcaaat cgtttctact
    38281 ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa
    38341 acaggaaaac agatctatta ttccaatact ggcaaaaacg aagtgattca aatattttct
    38401 tataacaaaa ataacaagca gaacaagcta caattaactg atggaaaact cccggaaaaa
    38461 ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt
    38521 tatcagattg attcagatga attgaaagaa aaagagtata ccatcacggg ctttgtccgt
    38581 tctccattgt tcatcaacaa tttggagaga ggatacgcga acgtcggaaa tggtagcgtc
    38641 gattattttg tctatttacc tgaatccgtt tttaaaacag atatttattc tgtcttatat
    38701 cttgatttta caaacgtaaa gaatttgaat acgtacagca aagcttacaa agataaaatg
    38761 gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc gacccgaaga acgcttagaa
    38821 gagctgaaaa aatcggccaa cgaagaatta gaaccggcac agcaaaaaat agcggatggc
    38881 aaagcacaaa caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa
    38941 cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta
    39001 tctcaacaag aagctcaact tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa
    39061 ctggcctctg cagaggaaga attgaatcga aatgcagaaa aaattgaaaa tttaactaaa
    39121 ccatcatacc tatacaatga acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg
    39181 tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt tctttttctt tattgccgca
    39241 ttgatcacct ttacgacaat gacacgtatg gtagaagaaa accgcaggga aatcgggaca
    39301 ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt
    39361 ctagcctcag ggataggtat tgttttagga acgatattag gaacgaatct tttgccaagg
    39421 atcatttttg agctgtctaa cgagcggtat gatattggga gtgcgcttat tttttatgac
    39481 tggccgccga ttttacaagc agctgccgca ttcttcattg ccgcatttgg cgctgcaatg
    39541 atcgttttat tcaaagattt aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg
    39601 aagccaggga aacgcatttt tcttgaatac atcactccgc tgtggtcacg ccttagcttt
    39661 aatcaaaaaa tcagttaccg aaatctcttc cgatataagt caagaatgct tatggcgatt
    39721 atcggaattg ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa agattctcta
    39781 agttctgttt cagccaaaca atttggaccg atcattgact atcaagcaat cgttacattg
    39841 gattcatcca cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa
    39901 gtaaatgaga tcctagctat tcatactcaa accgtagaat tgagaaaaaa aggacatgct
    39961 tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg atttggatcg ttttatccat
    40021 ttaactgatc ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa
    40081 aaagtagcag aactatttga cttatctaca ggagatcata tttcgcttta tgatgaagat
    40141 caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg tagctttatt
    40201 tatatgtccc ctgaatatta tgcttcagtt actgatcaat cgatgatgga taatgcattt
    40261 ttgctgcgta cagataagat gacaacaaaa gaagaagaaa tgctttccga gaaactgctt
    40321 gccacagacg ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa
    40381 tcaatggcga acttagactc ggttgtctta atctttgtcg tattatcagg actgctggct
    40441 tttatcgtct tgtataatct aactaatatc aatatttccg agcgtgtacg cgaattatct
    40501 acaataaaag tccttggatt ttttgataaa gaagtcacga tgtacattgt tcgagaaaat
    40561 atcatcttta ctcttttagg aatcatcgga ggatttggtg taggatatat attgactgac
    40621 ttcatcttgc aacaagcgtc tatggagaat gtcatctttc cattagtgat cacatggtgg
    40681 gcttatgtct tatcagcagg attaactatc gtatttacgg ttattgtgat gattgttacc
    40741 catttcaaat taaagcatat cgatatgatt gatgcgttga aatcgaatga ataaaaacag
    40801 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggcaa aatagttttt
    40861 catattttgc cgttttttga gctaataccg aaggacagct gattgaacac catgaatcag
    40921 aggttgtaaa aaagccgtcc tgcatcgagc aaaaagaata ctcaaataaa attagctcct
    40981 catgttttac atttgagaag actgattgcc gtagataaaa gcttttgaac accatttata
    41041 gaagaaacaa gaatattttg ctattttaaa caagaggata gacaaataca tgtctgatct
    41101 cttgtttttt attgggcaac agacctctta gaatgtataa agataaattt tacaaacgct
    41161 ttcggttcag ttataataat aaatgataac ttgtatatat aagttgtggt aggtaataaa
    41221 tcagttgaat ggaggtaaat atccatgaca tttaaagaca aagtcattta ccaaatctat
    41281 cccaaatctt acaaagacag taatggtgat ggaattggag atttaaaagg aatcaaagaa
    41341 aaactcccgt atctgaatga tctaggcatc gacatgattt ggctaaaccc tatttatcca
    41401 agccctcaaa aagacaacgg ttatgatatc tctgattaca cagccatcga tcctatcttt
    41461 ggtacgatgg aggaattcga acagctaata gccgaagcaa agaaataccg gatagaaatc
    41521 atgctcgata tggtgctgaa tcacgtttcg acagaacatg aatggttcaa aaaagcacta
    41581 gcaggggacc agcattatca ggacttcttc attttaagag aagagccaac ggattgggta
    41641 tcaaaatttg gtggaagtgc ttggtcgcca tttggagata cggggaaata ttatttgcac
    41701 ttatatgatc ggacacaagc tgacttgaac tggcggaacc aagcagtaag agaggaacta
    41761 ttcaaagttg ttcgtttttg gatggaaaaa ggggtaaaag gattccgttt cgatgttatc
    41821 aatgttatcg gtaaagacga acaattgaaa aataataaag aaaatgatgg caaacctgaa
    41881 tatacggaca aaccaatcac ccatacgtat ctaaaagaat tgaaccaagc aacgtttggg
    41941 acggattcca ctatcatcac agtcggtgaa atgagctcta cgacgatcga aaattgtatc
    42001 ttgtatacag aacctagtag agaagaatta tcaatggtct ttaatttcca tcatttgaaa
    42061 gtcgattatg aaaatgggaa taaatggtct actcctccat ttgattttgc agaattaaag
    42121 cgactatttc atacatgggg agaaaagatg agtgagggta acggctggaa tgctctattt
    42181 tttaataatc atgatcagcc acgtgcgctc aatcgatttg tagctatcga tcagtatcga
    42241 aaacaaggag cagagatgct agcaaccatg atccatttga accgtgggac gccctatatt
    42301 tatatgggag aagaaatcgg catgatcgac ccagactatt catcgatttc cgactatatt
    42361 gacgtagaaa gtttgaatgc ttataagtta ctccttgaac aaggattcac agaaaaagaa
    42421 gcgtttcgtc ggatcaaagc aaaatctcgc gataactctc gaacacccat gcaatggtca
    42481 gacacagaac aagcaggatt tactacaggg aaaccttggt taaaagttgg aggcaagcta
    42541 gaagagatta atgtagaaaa agaactatct tctgaagatt ctattttgtc ctattacaag
    42601 aaattgattc ggctgagaaa aacatatcca atcgttgcac aaggagatta tcatgcttat
    42661 gaagcaaatc atccggaagt ttatggcttt cttcgccaat ttgaaggaca gcagctgctt
    42721 gtattaacaa atttctatga taaagataca aggatcgcca tcccagaagg attcacagaa
    42781 gccaatattc ttatcgataa ttattccgat actgttcttc atcaaagtct ggagctaaaa
    42841 ccataccagg caattgcttt gctgaaaaat taaaacaaaa acacaaaacg tcgttcctac
    42901 gagctattct cgtaagatcg acgttttgtg ttttatttaa aataatcttc ttcagaaaat
    42961 gaagagtttt catactctcc tttatcgcca taaaatttat tgtagcgttg tttgaattta
    43021 cggaaaataa aagtaacgag taatttgact aacgtataga taggaatacc aaaaataacg
    43081 cccacaattc ccattagatc acccatgaca agtaaaacga ttaagatcgt aatcggatgg
    43141 atctgtaaac gatcacccat tactcgagga atcaccaagt caccgtggag aagttgtacg
    43201 gcaaaccaga caatgacaaa tttgacagcc ataaaagtag agtcttggaa tgcaatgatc
    43261 aaacctggaa agaaggcgat aaacggccca atatatggaa tgatacataa caacccagca
    43321 gaaatagcta aaacactagc aaattctaat ccgatcagta agaaacaaac ccagtagact
    43381 gctccaagga taaatgaagc aatgatttgc ccctttagaa atgaacccaa ttgttgatta
    43441 gcaatctttg tcaaattttt aaaatctgta cgaaatgctg gtgggacaat cgctaaaact
    43501 gactgataaa atttttgagg atctctcaaa aggaaaaaag cgacaattgg accagtaaat
    43561 aaagtaataa aaatagctgt aacagttgaa aaaatgttgc tgatccgttg tgcgccactt
    43621 tcccaataat ttccaataaa tccagctata tcagacgtca cgtcgtcaat agaagcgaaa
    43681 aattgattaa atgaatcggc aaaaggtgtt ttctccaaaa attgtcggag actctcttga
    43741 aaatcttgaa acaaatctgg caaactactg atcaatgtct ttgcctgttc ttcaagaaaa
    43801 ggaataagcc atatgcttcc aagccccaat aaaaataaaa taatcacata aacgataatg
    43861 actgacaagt tcctcgaaag gtgcttgctc aaacgtttga tgactggttc caaaagataa
    43921 tacaagatca gtgcaaacaa taatgggggc aagatagtag cgataatgat tccgacaggc
    43981 ttgaatataa acgagatctg atggaaaaaa taaatgatca gcgcaagtaa gataactatt
    44041 cctaaaacat agtaagaggt ttttcctcct aaaaagcgga tcaaacgatt cgttgtatct
    44101 gtttcttttt tcactctttt tcctccttta tctataactt cattatagac aaaagcgggt
    44161 ataaaaggaa aatgatagag gttgattgca aaaatatcgt caatcattca tataaacttt
    44221 ctcatcaatt aaaataagaa aacgcttgac agttctttta accatatgat aaattaaatc
    44281 ctgttgctaa aacgtaacta ttacggtttg cgaaaagcga atcggaacat tcaaatgaaa
    44341 gaagatgaga agaggagtgt cataaatgaa acgattgata ggtatattgg ccatgctggt
    44401 tttagcaggt atgttaactg cttgcggggc aagtgggaaa gccgaggaca gtaaagaaaa
    44461 attgagcgtg atgacgacct tttatcccat gtatgatttt acaaaagcaa tcgtcgggga
    44521 tgaaggagaa gtagagttat tgattccagc agggactgat tcccacgatt atgaaccttc
    44581 tgctaaagac atggcaaaga tccaagatac agatatcttt gtttataatg atgaaaacat
    44641 ggaaacatgg gtccccgcaa tccaaaaaac attacaagaa ggcaatgtcc atacgataaa
    44701 agcgacagaa ggtatgcttt tacttccggg aagtgaagaa ggacatgatc atgaccatga
    44761 acacggggaa gaaggccata cacatgagct agacccacat gtatggctag ctccaagttt
    44821 agcaatcaag caggtagcaa acatacgtga tcagctgatt gaagcttacc cagaaaaaca
    44881 ggaagtatgg acaaaaaatg cagcagccta taccgagaaa cttcaagcac tccatcagct
    44941 ttatcaagaa acctttaagc aagcaaaaca aagatctttt gtcacgcaac atgctgcatt
    45001 caactatcta gcgttagaat atggattgaa tcaagtatcg attgctggtc tttcttctag
    45061 tgaagaaccg tctgcggcga gaattgctga actgaaacac tttgttaaag aacatggaat
    45121 caactatatt tattttgaag aaaacgccaa agatagtatt gccagaacgt tagcaaacga
    45181 agcaggtgta tctttagaag tattgaatcc tttggaagga ttgacgaatg aacaaataga
    45241 aaatggagaa aactatcttt ctatcatgga agcaaacctc gaagcgttga aaaaaacgac
    45301 agagacagaa aacccgctag aagatacttt gacccccaaa aaagaaaaga gcgtctacaa
    45361 cggttatttt gaagacgagg atgtacaaga tcgctctctt tcagattggg caggaacttg
    45421 gcaaacagta gatacgtatg tagaagatgg gacttttgat cctgtttttg aatataaaga
    45481 aaaattgaat caggataaga cagcacaaga atataaagaa tattatacga agggctatca
    45541 aacagatatt gcttcaatca agatcagtga gaaagagatg gcatttacgt ttaagaatgg
    45601 aacaacgaaa cgttcaaatt atcgttatgt agggaaagaa atcttgacct acgaggcagg
    45661 aaaccgtggc gtaaggtatc tattcgaagc agaagataag gaaagtggcg cttttagata
    45721 tattcaattc agtgatcaca cgattgcacc ggctgactcc gaacattttc atttatatgc
    45781 aggaa
//
LOCUS       DAYYAJ010000007        45670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000007 DAYYAJ010000000
VERSION     DAYYAJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.007"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..675
                     /locus_tag="ACHATZ_000357"
     CDS             <1..675
                     /locus_tag="ACHATZ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8402918.1"
                     /translation="VAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVI
                     ISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYF
                     ASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYN
                     IPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFD
                     SDSALF"
     gene            687..1607
                     /locus_tag="ACHATZ_000358"
     CDS             687..1607
                     /locus_tag="ACHATZ_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8402919.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1648..3105
                     /locus_tag="ACHATZ_000359"
     CDS             1648..3105
                     /locus_tag="ACHATZ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8402920.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3450..4064
                     /locus_tag="ACHATZ_000360"
     CDS             3450..4064
                     /locus_tag="ACHATZ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8402921.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4057..5781
                     /locus_tag="ACHATZ_000361"
     CDS             4057..5781
                     /locus_tag="ACHATZ_000361"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8402922.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5778..7229
                     /locus_tag="ACHATZ_000362"
     CDS             5778..7229
                     /locus_tag="ACHATZ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8402923.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7702..9855)
                     /locus_tag="ACHATZ_000363"
     CDS             complement(7702..9855)
                     /locus_tag="ACHATZ_000363"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8402924.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9857..10903)
                     /locus_tag="ACHATZ_000364"
     CDS             complement(9857..10903)
                     /locus_tag="ACHATZ_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8402925.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11053..12342
                     /locus_tag="ACHATZ_000365"
     CDS             11053..12342
                     /locus_tag="ACHATZ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8402926.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12355..15057
                     /locus_tag="ACHATZ_000366"
     CDS             12355..15057
                     /locus_tag="ACHATZ_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8402927.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15042..15899
                     /locus_tag="ACHATZ_000367"
     CDS             15042..15899
                     /locus_tag="ACHATZ_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8402928.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15970..16827)
                     /locus_tag="ACHATZ_000368"
     CDS             complement(15970..16827)
                     /locus_tag="ACHATZ_000368"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8402929.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17038..19209
                     /locus_tag="ACHATZ_000369"
     CDS             17038..19209
                     /locus_tag="ACHATZ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8402930.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19277..19618
                     /locus_tag="ACHATZ_000370"
     CDS             19277..19618
                     /locus_tag="ACHATZ_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8402931.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19894..21648)
                     /locus_tag="ACHATZ_000371"
     CDS             complement(19894..21648)
                     /locus_tag="ACHATZ_000371"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402932.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21659..22495)
                     /locus_tag="ACHATZ_000372"
     CDS             complement(21659..22495)
                     /locus_tag="ACHATZ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8402933.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22497..23180)
                     /locus_tag="ACHATZ_000373"
     CDS             complement(22497..23180)
                     /locus_tag="ACHATZ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8402934.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(23339..24733)
                     /gene="uxaC"
                     /locus_tag="ACHATZ_000374"
     CDS             complement(23339..24733)
                     /gene="uxaC"
                     /locus_tag="ACHATZ_000374"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8402935.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(24772..26271)
                     /locus_tag="ACHATZ_000375"
     CDS             complement(24772..26271)
                     /locus_tag="ACHATZ_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8402936.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(26264..27685)
                     /locus_tag="ACHATZ_000376"
     CDS             complement(26264..27685)
                     /locus_tag="ACHATZ_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8402937.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(27745..28737)
                     /locus_tag="ACHATZ_000377"
     CDS             complement(27745..28737)
                     /locus_tag="ACHATZ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8402938.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            28969..31167
                     /locus_tag="ACHATZ_000378"
     CDS             28969..31167
                     /locus_tag="ACHATZ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8402939.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(31625..32497)
                     /locus_tag="ACHATZ_000379"
     CDS             complement(31625..32497)
                     /locus_tag="ACHATZ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8402940.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            32797..33771
                     /locus_tag="ACHATZ_000380"
     CDS             32797..33771
                     /locus_tag="ACHATZ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402941.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            33793..34485
                     /locus_tag="ACHATZ_000381"
     CDS             33793..34485
                     /locus_tag="ACHATZ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8402942.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            34451..35959
                     /locus_tag="ACHATZ_000382"
     CDS             34451..35959
                     /locus_tag="ACHATZ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8402943.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            36033..37136
                     /locus_tag="ACHATZ_000383"
     CDS             36033..37136
                     /locus_tag="ACHATZ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8402944.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            37139..38476
                     /locus_tag="ACHATZ_000384"
     CDS             37139..38476
                     /locus_tag="ACHATZ_000384"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8402945.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            38463..39341
                     /locus_tag="ACHATZ_000385"
     CDS             38463..39341
                     /locus_tag="ACHATZ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8402946.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            39338..40261
                     /locus_tag="ACHATZ_000386"
     CDS             39338..40261
                     /locus_tag="ACHATZ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8402947.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            40258..40668
                     /locus_tag="ACHATZ_000387"
     CDS             40258..40668
                     /locus_tag="ACHATZ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8402948.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            40672..42240
                     /locus_tag="ACHATZ_000388"
     CDS             40672..42240
                     /locus_tag="ACHATZ_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8402949.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            42480..43898
                     /locus_tag="ACHATZ_000389"
     CDS             42480..43898
                     /locus_tag="ACHATZ_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8402950.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            43910..45220
                     /locus_tag="ACHATZ_000390"
     CDS             43910..45220
                     /locus_tag="ACHATZ_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8402951.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        14833 a         8039 c         9340 g        13458 t
ORIGIN      
        1 gtagcattta tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag
       61 ttgagaaata aacgactcgt taaagtttat caaacgatgt ccttactacc ttattttcta
      121 tcttgggtca ttatcagtta ctttgtttac gcttttttaa gtcctgacaa aggaatattc
      181 aatcagtgga tcactggaca tggaggagaa gctgtcaatt ggtataatga accgaaatat
      241 tggccgttta tcttggtgtt cattggtaca tggaaaggga ttggttataa cagcattatt
      301 tactttgcat ctgttatggg aatcgatccg acttattatg aagctgctat ggttgatgga
      361 gctagcaaat ggcagcagat caaacatgtg acgataccac aacttattcc actaatgacg
      421 attttaacga ttttagccgt aggaaacatt ttccgagctg attttggcct gttttataac
      481 attcctagga attccggtgc attatatgat gtgactcaag ttttggatac gtatatctac
      541 aatggcctga catccacagg agattttggt atgacggctg ctgcaggact ttaccaatca
      601 gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt tgatagcgat
      661 tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc
      721 gctctttcaa taagacggct aattttttct ctaatataat gattgcactc tttgctattt
      781 cctgtatttt gccatttata tttgttatcg ccatctcttt taccgatgag tccatattgt
      841 cgactcacgg gtacagtttt tggccagaaa agttcagtat atttggttat acgtacttgt
      901 tcgaccagat gcaggataaa attttccaag cgcttttcgt cactgtactt gttactgttt
      961 taggtacact gatcaatagc acggctactt ctttatatgc ttatgcgatt tcacggtcaa
     1021 actttccatt ccgtcgtttt tttaccgttt tttgtttaat tacgatgttg ttctcaccag
     1081 ggatggtggc caattatctt gttatgacaa atcttcttca gttaaaagat acgatttggg
     1141 cattgatctt gccaatggct gtcagtccct tcaatattat tgtgatgcgc acattcttta
     1201 aacgttcggt ctctgattcg attattgaat cggcacggat cgatggagct agcgaactgc
     1261 gaatatttgt tcagatcgtt ttaccgttag ctgttccagg tattgcgaca atcagtttat
     1321 ttgccgcatt gggttactgg aatgactggt tcaatgcttt actatacatc caagatgata
     1381 agctagttcc actacagtat cttttgatga aaatccaaag taatattcaa tatctgacac
     1441 aaaattcagg agcaggaagc caattgacag gcggattagc ctcagttcca ggagaatctg
     1501 ccagaatggc gatcgtagtc atctcgacgt tgcctattgc gatcagctat ccatttttcc
     1561 agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga aacaaaaata
     1621 taaatcataa aattaggagg aagaaaaatg aagttatgga agaaactagc ctttacaggt
     1681 gtttcagcgt tgttgttggg aacattagca gcatgcggag gttctggttc taaagaccag
     1741 gcagaagcta gcaattcgga cactttgcag atgtatcaaa tcggagataa accagataat
     1801 ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc
     1861 aatatcaatt acattggctg gggcgattac gaaaagaaaa tgaacgtcat catttcttcc
     1921 ggggaaaatt atgatatcgc ctttgcgaac aactatgttt ccaatgccca aaaaggagcc
     1981 tttgctgatt tgactgaatt agcgcctaag tacgctgaaa aagcttataa tgatttagat
     2041 gaagcatata ttaaaggaaa tctggtcaat ggaaaattat atgctttccc agtcaatgga
     2101 aatgtctttg ctcaacaagt acttactttt aacaaagctt tactagataa atacgatctt
     2161 tcaattgatg gaatccaatc ttacgctgat gcagaaaaag tcttgaagga attccatgaa
     2221 aaagaaccaa acacggcagc ttttgccatt ggacaaggct tcaaagtcca agggaacttt
     2281 gattatcctt tagggaatac gttaccattt gctattgatc tggatggaga tgattcgaaa
     2341 atcatcaacc aatacgataa tgaaacgtat atcggactgt tacgtacaat gcatagctgg
     2401 tacaaacaag gactcattcc aagtgacgct gcaacgagca ataaggatta tccactagaa
     2461 ggaaatactt ggctaatgcg tggagaaaca caaggaccgt atgattatgg cgatacaatt
     2521 ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc actaaaatct
     2581 acagctcaag cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa
     2641 tcagtagaac ttctagggct gcttaacagt gatccagaat tattgaatgg gcttgtttgg
     2701 gggatcgagg gagaagcatg ggaaaaagtc gatggttcag atgataaaat caaattgcta
     2761 gatggttatc agccaaatac acatatgtct gcttggaata caggaaacaa caagattcta
     2821 tatacacaag agtctattac ggatgacatg attgccaaac gtgatcaatc gattgctgat
     2881 gcagaaactt caccgatttt aggattcagc ttcaacactg acagtgtcaa aacagaatta
     2941 agtaatattt ctaatgtcat gaatcaatat ttagatggat tgaatacagg gactgttgat
     3001 ccagatgaaa cattaccgaa attgaaagac gctttggaca aggctggcta tgataaggta
     3061 ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag aataataatg aataaagaca
     3121 aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag
     3181 ccagtttctc ggtattaagc taaatatatg taaaaatgtg gaaagccata ttcacaaaat
     3241 ttttcttact actcgaaact aagcggctat ttcacaaact cttggtaaac ggtgttcaat
     3301 cagctaaccc tcggtattcg ctcacaaaac gacaaaatat gaggaactat ttttccgttt
     3361 gtttctctaa taatcaggtc aaggtgctga ttgcccaacc tttttttgta taatgagaaa
     3421 gtataaactt aatgtttgga ggagaagata tgttagggaa ggttttggaa gagttattta
     3481 tccggatctg ggtcattata aaactaacct tgtatttctg gatatataca tttgccggcg
     3541 ggatcatctt tggattaggt gcggcatgga aaacagtcaa tgaactattt tatttgtacg
     3601 gattcgagta taaagaaatc acaataaaaa gaggctggaa tatatataaa agaaattttt
     3661 taagaggcaa tctgctattt agtttatttt tatctggtac tgctttattg agttacaatt
     3721 tatatttatc tgttcaaatc aaagggctca tttttttagc tattgatttt attttacttt
     3781 ttgccttgct ctgcctattt gctacttatc aatacagtat gattttagat agtgaatata
     3841 cgatctctgt tcctaatctg ctaaagctaa gttttatttc ggttttttcc agcttttcgt
     3901 cttttctaaa aataattatt ggtagcggta taattttagg cgtgacttgg caattcaaag
     3961 gattgatcct gtttggtgtc attggtcttt tgacggtttg gaatggtacg atgacaacac
     4021 attggcgtga agaattagat aaacagttgg aaagttatga ataagtttcg gattctattt
     4081 tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg cggttatcat gatcggtgta
     4141 acgatgactg ctaccgttat ttttagtatc catacttggg aacaaaatca aaaacaggca
     4201 gaaaacatga caagtgatgc cgttcaaagc acaagcagaa tgctgaatga taaaacaacc
     4261 ttgagtagga tcatcaaaaa ccagcttgta gggaattccg aaaagatcga aaatgtgacg
     4321 acctacttaa caaaacccat agatcagtat ctgatgtatg tatacgaaca gcaaaacagt
     4381 accgatgaat tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa ttatgaagaa
     4441 ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt attatgaatc ttctagagaa
     4501 aacaaaggtg gggaaataaa aaaggggaca ccaaaattac tagacgcctt ttatatcaag
     4561 ttgcctatca cacaaggagg agctgagtca ggaagtattt ttattggttt tcaaaagaaa
     4621 gaactagata tgattttaga aaatctaacg tcattcaatg gactctcttt atacatgatt
     4681 tctggaacga caaatcgact atacacattc catgataata aaatagctaa agaaacgttt
     4741 gaaagacaag aaatgatcat cacagagagt ctgaagaaaa attcacaatt gccgattgac
     4801 gatctggaaa aaaacaattt tgttcaacac caagaactgt ctgatgatta tcgtatatta
     4861 gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc
     4921 attggtatca ttattttaga caatatactg cttatctttc tttataaaac attcaaacga
     4981 tattcacagc aagtatctat cgtcatgcag gccatggatc aaacagcagc agggaattta
     5041 gagacgcgga tcgacataac aaacacggaa tatgaactga aggaactttc aatcggtatc
     5101 aacgaaatgt tggatagcat caatcagttt gtagaagaca tctacaaatt agaaatcaaa
     5161 cagcaagacg cacatatgcg ggcgcttcaa gcacagatca atccacactt tttatataat
     5221 acattagaat atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt
     5281 gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc aagagaaaac gatcacttta
     5341 aaagaagaac tggacttttg cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat
     5401 cgagtagcct atcattttat gatcgatcct gatttggaaa agatcgaagt acctaaattt
     5461 gtgatccagc cattagttga aaattatttt aaacacggta ttgattttac tcgattcgat
     5521 aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat catcaaagat
     5581 aatggaaaag ggatgactga aaaacggcta aaacagattg aagaaaaact gtctcatcct
     5641 aaagtggaac ttcacggttc tatcggtctg caaaacgtga atgaacgttt acgtgccagc
     5701 ttcggcagca gttattatat gtcgcttgag aataatgaga caggtggact gaccgtttct
     5761 attacattca aggagggatg agcgtgtatc aagtgttatt ggtagatgat gaatacatga
     5821 ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt aggctttcaa atagtagcaa
     5881 cagcagagaa tgccaaagaa ggactagctg tacttgaaca gcgacagatt gatttagtag
     5941 taacggatgt gacaatgcca gaaataaatg gactggaatt tattgaagcg gctcaaaaag
     6001 aacgtcataa ttttgaattt atgattttat caggatacca agaattcgat tatttgaaag
     6061 gaggtatgca attaggcgcc gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg
     6121 ttgaaagcct caagaaaatc aaaacacggt tagatcagca aaatgaacaa aaaaatcaac
     6181 aagaaatcta tcaagaactg ttattcagtc aatggttgaa tgaagaattg gatgaaataa
     6241 gtgaagaaga aataatagga caaataagtg agaagcagca taggatagta ttgattcaac
     6301 tggcaagaac tcatgatact cttatcaata gatggttgca agaacggcaa caaatctttt
     6361 actatcaacg aaattacgga gaccgtgtat tgatcacgtt gttgttggag gcagatgaag
     6421 ttgatggctt ctgttgtttt gttcaagaaa actttcctga tcaggaatgg ctgatcagta
     6481 tcggggaaga gacagattct ttggacaaaa tccctgaaac attccaacaa gcgaaagaca
     6541 atctacagtt gcatcaattt tatggagata aagacgagca tgttttttac gcagtacaag
     6601 cagaaacaaa agatcaaacc atcgattttt caagtttcag acgcgtgctg caaaataaac
     6661 aattggaagt cgctcaaaaa atgatcactg atttttttga acagtttcag ttagcagcca
     6721 tgatgccgga agatatacga tattccagtt ttttactgtt tatggagatt caacgggaat
     6781 tgattgatct ggaagatgag gaatatttac aaggaatcga aaaaatccat caggcaaaaa
     6841 cggtgcaaga acttcagcag ctacttctgt cattcatcaa aaagcatcag agacagaaaa
     6901 agtacagtga caatgtggaa aaagtaatcg taatcctgca ccagcattat caagaaccac
     6961 ttacactcaa agaagtgtca gaaaacctgc atttaaatgt catgtatcta ggacagctgt
     7021 tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa ccatttacgt atggaaaaag
     7081 cgaaacaact gcttttgcac agtaatcaaa acatcaatga gatagcaagt gagattggct
     7141 acaacaacac cacgtatttc tctaaattat tcaagaaaat cgtcggcaga tcccctaaag
     7201 agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc aatgtaatga aataacttat
     7261 aaaaaattgt ctagaaatct aattttgggc tgttcttatt acttgaggca aggcagcttt
     7321 ttcatgacct ctcgataaac ggagttcaaa agccagtttc tcggtattaa gccaaattta
     7381 tgcaaaaatg tggaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct
     7441 ttttcacgac ctctccacta aatggtgttc aatcagctga ccctcggtat tagctcaaaa
     7501 aatggcaaaa tatgaggaac tattttgcca tttgtctctc taatactcag gtcagaacgc
     7561 tgatttcaca acctctcaat aaacggtgtt caaaagcttg catctgcggt aataagacca
     7621 accaaacgaa aaatatgagg atctattttt gcttggctgt tcttattact tgatgcagga
     7681 cagctttttc acaacctctt gttaattata gtttgataag gaaaaaggca aatcttttgc
     7741 ttgatatttc ttagggtgag ggatgatccc taattggtaa gagatcgtgc cgcctttcag
     7801 caaatcttga tgggtaaaat ataatttcgt atattctctg tccatccgtt tgattttatg
     7861 gataaattgt tgctgcggcc gattaggcgt tgcttcaatc gtcaattcct ttccatttga
     7921 caaaagcaaa acagccttgt caattaatgg cataccgata acatattcac cggttccagg
     7981 cgtgacagga taaaatccca gactattgaa gatataccaa gcggccatac ttccattgtc
     8041 ctcatctcca gggtaaccca ttggagaatc gtcgaaggtc tgagtcatca actgtttaag
     8101 cagtggctgt gccatttcag gttgtcctat ataactgaac aagtaaggat agtgaaaact
     8161 tggctggttt gaaatagcta attgtccaaa ttctatcgcg gccatctcac tcatttcgtg
     8221 gatctcaaaa ccatatcctc ctgggtcaaa cgttgggggc tgattgcaca gctcaatgag
     8281 tttttcttta tatctctcac tgccaccata ttttccgatc agtcctgaaa aatcgtgata
     8341 aacagcaaag ctgctttgcc aagcacttcc ttctgcataa tcttgtcccc aacgattagc
     8401 tgaaaatggt tctctgaact tgccatcttt atcttttgaa cgcataaatc ctgactcata
     8461 gtcaaagata ttttgatagt tcaatgattg ctgctgatat ttcttcgtca tttcctcttc
     8521 ttctagtacc ttggcaactt ggctaataca aaaatcgcta taagcatagt ctaatgtgtg
     8581 attgactgat tcgtggtagt gattaggaac atagccatat gtcaagtaat cttctgttcc
     8641 ttggcgtcca taatttggat gtttgctttg aaccgtagca cctttttcca ttgccgttag
     8701 aaattcaggc atcaagtctc gtcggatatc ttttacagca gcatctgcaa tgaccgcatc
     8761 gatcaatgtc ccaggcataa gtccacgttc atcaggagac agccattttg gcaaatagcc
     8821 actttctcta tagctgttca aaaaaccttc cagcatttct tcgtattttt ctacagcaat
     8881 caaagaataa agaggataaa ccgttttata cgtatcccaa aacccattat ttgtatagag
     8941 aacaccaggt tttatagatc gactagtcgt atcatagtgg attttttgcc cactaggatc
     9001 aatttcataa aatgtttgtg gaaataaaaa cacccgatat aaattatgat aaaacgtccg
     9061 caattgttgt tgatcatggt gtgtgagctg tatacgatca aaataggact gccattgttc
     9121 ttgactgtga gcaaggtaag tctctttgct ttggttttgt tctagttcca ggttataagc
     9181 tgcttgttcc gagctgataa aagatgtccc aaatcggata gtttgttttt ttgtctgttc
     9241 aaaatacaac gggatcgagt cgctattttt gttggaaaca gctaactcat caattgttaa
     9301 aggatattca aatctcaata taaaataaaa agaaaaatca ggatcttctg aaccagcaaa
     9361 atgtgtgatt tttccttcca ctgtgtaagg atctataaca aagagctgat ggcgtcccgg
     9421 aaaggataaa agtaaaccgc tgtcattttt gctgtaatca atagataaga ttccgccata
     9481 catgctaggt atcaatgtag aacagatgcc ataacgtaat tgacagatcg acagatgagt
     9541 cgggcaaaat atactttctt ctgggcggta agaactttga gcatggaaaa gggtgttctc
     9601 ctgtagtttt ccattgatcg gcgtaaacag catatgactg aaatcaccca tccaaggact
     9661 tggttgatgg gtaagacggt agccttgaaa aacgcggtca tcaggatgaa accaccagct
     9721 gccgttttga tccgtagttt gcggtgcaaa ataattcatc ccaaaaggaa cgccagtata
     9781 aggctggcag ttcccattag agaaatttgg ctgattcgct gttgcatgtc tagtatctat
     9841 ttgaccgata ttcattttat ttctcctttg ttgattgtct tttgatgaat gaaactggaa
     9901 atttactatc ggatggtcga tcttttgtct ctaaccaagt gaccaaagca tctcccgcaa
     9961 gcttacctaa ctgttcaaaa tcttgtgcga tggttgtcag tggcgggtca acaagagctg
    10021 ctgcttggat atcatcaaat ccgataattg aaaaatcttc agggatatga taaccatgct
    10081 gtctcaagag attcatggtc tggatagcga caacatcgtt ttcacaaaca aaagcagtta
    10141 cttcatactt agtgatataa gaaagcaatt catctaatgt cgcgcttttg tcatccagtg
    10201 aagtgtggaa agtcaaacca gctttgtcca atgcttcaag gtaaccaagg taacgctgtc
    10261 gaacggattg tggatgagtc tgcgtaccaa acaaataagc gatttttgaa tgtccctgtt
    10321 cgatcaaata agaggcagca agcgcaccgc cttggaaatt atcggaaaga acggtaggaa
    10381 aaggtaactc atagattttt ttatctaata tgacgacagg gaattctttc aatgaaagct
    10441 caaaaagtaa gtcaagatgt tgttctgtat caagagcata ataaatcaat ccgtcaaatt
    10501 cattgattag ctcttttgct gttttatttt gtagaaagtc aagcgtcgtc atcatgacat
    10561 caatctgttt atcttgcatc acagggttca atcccttgct aaaatcacca acagacaaat
    10621 cagcgacata tggaagaaga aacagaatcc gtcgtgtttt tgtaggagaa gcaagaatct
    10681 gttccggaga agcggattta acgaaactgc ctttcccgcg aacgcgataa ataagtccta
    10741 attgctctaa ttcagtcaat gcacgtttgg aagtgattct gcttacttta taggtacgtg
    10801 aaagttcttt ttcagttggt agttggctgt ctacaggtaa ttcaccagtt aaaatagccg
    10861 tttgcaaatc attaaatatt gtctggtaca atggttgagt catataaaca cctcttaatg
    10921 atatatcata ttcaaataat accataatgg atgaaaaagt aaataaaaag atattttttg
    10981 aaagcgtaga cataattggc tgtatgaagt ataatcttta aagatatatc aaatagaaag
    11041 aaggttttat tagtggttta taaagatata ccaaaatcgg tgcagaaatt tatggaagat
    11101 atcacacaaa aatgtgggga tatccatgcg gactgggcaa agaattttac agcagcattt
    11161 gccaatactc tgttgacaac agtcaaaaag caaaatgacg gaaccacctt tttattgacg
    11221 ggagacattc cagcgatgtg gttaagagac tctactgcac aagttcgtcc ttatttagtg
    11281 attgcaaaag aagatgagga attagctgac atgatcagcg ggttagtcaa aaagcaattt
    11341 ttttatatca atatcgatcc atatgcgaat gccttcaacg aagaagctaa cggtgctggc
    11401 caccaaacag atcatacaaa aatgaacgat tggatctggg aaagaaaata tgaaattgat
    11461 tctttatgct acccaataca gttggcctat cttctttaca aaaatacagg aaaaactgat
    11521 caattcaatc aatcttttat tgaaggtatc aaaaaaatct tggacgtgtt caaaacagag
    11581 caagatcatg aacactctcc ttatacattt gtacgagaca cgacaagaaa agaagatacg
    11641 ctgatcaatg acgggcgagg tgcaaaaaca gcttttacag gaatgacatg gtctggtttc
    11701 cgtcctagcg atgatgcttg ccgttatgga tatctggtac catccaatat gtttgcggtg
    11761 gtcgtgttag gttatgtgga agaaattttt caaacgatac tcgatgataa agaaattgta
    11821 cagcaaacac agaaattgaa gaaagagatc caagagggga ttcagaatca cggacgaacg
    11881 gttaatcaaa aagggcaaac gatttatgca tatgaagtag atggaatggg aaacgccaca
    11941 gtcatggatg acagtaatgt gcccaatctg atcgccgcac cttacttggg atattgttct
    12001 gaacaagagg aagattatct gcaaacaaga agaacattgt tgagtaaaga aaatccatat
    12061 ttctatgaag gaaagtacgc aaaaggaatt ggatcctccc atacgccaga aaaatacgtt
    12121 tggccgattg cattagctat ggaaggaatg acaacgtcag acaaatctga aaaagagcgg
    12181 atattgaatt tacttgtagc aactgacgca ggaacacacc ttatgcacga aggattcgat
    12241 gtgaacaatc ctgaaaatta tacgagagaa tggttttctt gggcaaatat gatgttttgc
    12301 gaattagtca tggattactt tgatattaga gtagaaaagt aggaggtatc gtaaatgaaa
    12361 aagaaagtat atatcatttc tcatagccac tgggatcgtg aatggtacat ggcttacgaa
    12421 cagcatcaca tgcgactgat tgaattgata gacgatctac tagagctatt tgaagttgat
    12481 ccgtccttca acagttttca tttggatggg caaacgatta ttttagatga ctatcttcaa
    12541 gtaaggccag aaaaaagaca ggcaatccaa caagcaatca atgaaggaaa attgagaatt
    12601 ggaccatttt atatcttgca agatgatttt cttatcagct cggaatcgaa cgtgagaaat
    12661 atgctgattg gcatggaaga aagccgtaaa tggggaaccc ctgtcatgtt aggctatttt
    12721 cccgatacat ttggaaatat gggacagacg cctcaattaa tgaaacaagc tggtatttca
    12781 gcagcagcgt ttggccgtgg tgtaaaacca attggctttg ataatcaagt tttggaagca
    12841 gaaaactatt cttcccaata ctcagagatg tggtggaaag gccctgatca aacagcaatc
    12901 tttggtctgc tatttgccaa ttggtatagc aacgggaatg agattccagt agaaaaagaa
    12961 gcagctcttg ccttttggaa gcaaaaatta gcggatgcag aacaatatgc ctctacgaac
    13021 cacttattga tgatgaatgg tgttgatcat caaccggtac aaaaagatat atcaaaagca
    13081 atccatctcg caaatgaact gtttccagat tacgagttta ttcattctaa ttttactgac
    13141 tatctggaag cagttcaaaa agatgtgcca gaagacttag gctcagtaga aggagaattg
    13201 acaagtcaag aaacggatgg ctggtatact ttagccaata cagcatctgc tcgggtttac
    13261 ttaaaacaat ggaataccaa ggtccagcga cagctagaaa atattacaga accacttgcg
    13321 acgatggctt atgaagttag tggaaactat ccccatgatc aattagatta tgcttggaag
    13381 acattaatgc aaaatcatcc tcacgacagt atttgcggtt gttcagtcga ttctgtccat
    13441 cgggaaatga tacctcgttt tgaaaaagca gatgaagttg gaaagtatct tgcacaagat
    13501 tccttagaac agctgaccgc agcaatcgat acgaccggat tcccaaaaga cagctttcca
    13561 tttgtcattg taaatactgc aggaatggat aaaactggtg aagcagaaat aaccatcgaa
    13621 ttagaaagaa aaagattcgc tgaaggaata cctgaacaac tatatcaaga gttagaaaat
    13681 ttgcctaaac gtaagtatca tgtagaaaca aaaagtgggg ccactatacc agctatttta
    13741 tctgaagaaa ctgtccaatt tggttatgat ttgccgaaag accgtttccg tgtgccatat
    13801 atggcaagaa tgatcaaagt gactttacca ttagaaaaca tgccagcatt ttcttgggaa
    13861 acattcgcgc ttgtagaagg ggaagcgaaa gcagaagaaa aggaaacaat gattcatcag
    13921 agtggacgaa tcatcgaaaa cggccctcta catttgacga ttgaaaaaaa tggtaccatt
    13981 acaatggaag atcgcaaaaa caaacgaaag ttgaataatc tgcatatttt tgaagatatc
    14041 ggcgatatag gaaatgagta tatctttaaa caacctttct gtgatcagcc aatcctatcc
    14101 agtaacaaag aaaactctga agtaaaagta ttagtagata caccggaaat agcaaaaata
    14161 agtattctac aggaaatgga aatccctgtt tctgcagacg agcgtttaga aaaagaacag
    14221 cagatggttg tggaatttcg ttatcgaaaa gcagaacgat ccaaagaaaa acgaatcctt
    14281 caaataaaaa caatcatgac aatcagaaaa gatagtaaaa aaatcgactt tgaaacgact
    14341 ctcgacaatc agatgaaaga tcatcgctta cgtgtcttgt ttcctacaaa gttacatgtg
    14401 gaacatcacg aagcagatag tatttttgaa gtggtgaagc gcccaaacca tgtgtctaaa
    14461 agttgggaaa accccacgaa tccgcaacat cagcaagcat ttgtcaatat tcatgatgaa
    14521 gagtacggtg taacagtcgg aaactttggt ttgaatgaat atgaagtaac agaagacgga
    14581 caaatcgcag tgacgctact tcgcagtgtg ggagaactag gcgattgggg atattttcca
    14641 acaccagaag cacagtgtct aggtgagcat cggttcaatt acagcattga actacatgga
    14701 ccagaagaaa aattctctac ttatttgcat gcatacgcag cacagattcc tttttccact
    14761 caacaaatca aacatcatga aggaacattg atttcaaaac aacaatattt aacgataaaa
    14821 agtgaaacgt ttgcaattac agctttgaaa cgcagcaagt ttagtgataa agtagtagta
    14881 agaggtttca atatgtctag tcatttagaa aaattggaaa taactaaaga caatggaaaa
    14941 actgtcatat tgaatctgct tgaagaacca acgaagcaag cggttgtccc aataatccaa
    15001 ccttatgaga ttcggacaat cggattcgag gaggaaaacg aatgtatggc ggaatagaag
    15061 caggcggaac aaaatttgtg tgtgcggttt cagatggtgg agaaatttta gaaaaattaa
    15121 gtatccctac cacaacacca gaagaaacat tggatcaggt atttgaattt tttgatcgtt
    15181 atgaattgaa gggaatcggg attggctcat tcggcccgat tggtatcgac aaaacaaaag
    15241 ataattacgg atacgttttg gcaactccca aaaaagggtg ggcacaattc gattttcttg
    15301 gcacaatcaa ggaacgctac aatattcctg ctgcttggac aacggatgtc aatgctgctg
    15361 catatgggga attgaagcaa ggagcagcac aaggaaaaaa tagttgtatt taccttaccg
    15421 tgggaacagg aatcggtgca ggtgtcgtga tcaatgaaga aattttttca gggatcgcac
    15481 atcctgaaat gggacatatt tgggtgaaac gccatccaga tgaccattat gaaggtactt
    15541 gtccttatca taaagattgt ttagaaggat tagcagcagg accgtctatt gaggcacgca
    15601 cagggatcaa agggcaagat ctgccgcaag atcatcctgt ttgggctatt caggcttatt
    15661 atattgctca agcactgatc aactatacgt tgacgttagc acctgaaaag attattttag
    15721 gcggtggggt aatgaatcag gatcatctcc ttcaaaaaat ccgtcagcaa tttgttgaat
    15781 tgatgggtgg ttatatggaa acacctcaag taagtgaata tatcgttcgt tggggactgc
    15841 cgaatgaaag tgggattatc ggtagtttgc tgttagcaga acacgaacat caaaagtaag
    15901 cagacaaatt acacaaaaag aacagagtct tatcattatc tgctgataag actctgttct
    15961 tttttatttc tattcccata atccaggtaa agctttaccg actatttttt cttgagtaaa
    16021 tggatggatc atgtgcagtt ctaatgcatg gagcatcagt cgttcagctg ttctattttg
    16081 gtagagaggg tctccaacga tcggatgtcc cttatgagct aaatgaactc tgatctggtg
    16141 tgtacgtccg gtttccaaaa cacaatagac ttgtgtttgt tcagaaagta ctttgttgac
    16201 agtgacatgt gtcaaggcgc tctgaccatt tttatcatct acgacccttt ttctccgatc
    16261 atggcgatct cgtccaattt tattggtgat ggtcatatct ttggcaattt ctccccatac
    16321 gatggcttga tatctccgat agatcatttt ttgttccaac attcttccaa gtatcggcaa
    16381 gacaaatgga ttttttgcaa ataaaacggc accgcttgtt tctttgtcca agcggtggac
    16441 aacgtaaggt cgttgctgtt tagtgtcgag ataagcagct aaatgattga gtaaggtact
    16501 gttttctgtc ggttcatttg gatgagtctt cattcctgca ggcttatcca ctataatcaa
    16561 atgctcatct tcatataaaa cacggatgtg cttcgcatct cctaattgaa cttcttgata
    16621 attataatct gtttcttcga ataacaatgt gatctggtca ttgcccttta cttcatgatg
    16681 gaacaaagca ggttgttgat tgatccaaac gtttttacgt atacgcaaaa agtgtcgaac
    16741 ctttctagga actaaccatt ccttttcaag caagtcgcga attgttgttt caggatggtt
    16801 cataggtaaa gtgatcgtga ttttcacaag tgtactcctt tcagtgcaat cgcagactaa
    16861 ttgtaccatt tttaaaacat atttaagaaa aaacttacat ttttctgttc attcttatgt
    16921 tactctactt gcactaacgt aaaaaaacat attgaactac aacttaagca ggaagaaaac
    16981 actctttcac tatggtaaaa taagacgatt gaatcaaata gacaggagaa gaagcaaatg
    17041 gattttcaaa gcattttaga aaagataaaa gctgctttga tcagtttttg gcgttggatc
    17101 agaccatatc tcggacaatt ccatcgatgg cgaaagcgca tctggaaaaa atatcatgtg
    17161 aacaaattga ttttgctgat tggtttgatc tgtgtcttag tgacaagtat ctacctcttt
    17221 attttagcaa aacaagcaaa tgtcgaaacg ttaaaatcag gattaagcca atcgacagtc
    17281 atttatgata agaaaaacga agaagctggc acactgtacg cccaaaaagg aacatatgta
    17341 gaattaaacg cgatttctcc ctatatccaa gatgcagtaa tctcgacaga agataggaat
    17401 ttttacgagc atcacgggtt tgatatcaaa gggattgccc gtgcagcagt ccgaatggtc
    17461 ttgtctggcg gaacatcagg gggcggagga agtacgatca ctcagcagct tgccaaaaat
    17521 gcgtatctga cattagacca aacgtttgat cgaaaagcga aggaattatt tttggcaatc
    17581 gagatcgaaa agaagtacag caaagaagaa atcttgacca tgtatcttaa caatgcctat
    17641 tttggcaacg gggtatgggg tgtccaagac gcttccagaa agtattttgg agtagatgca
    17701 acgaatgtta cgctgtctga ggctgctact ttggcaggaa tgctgaaagg tcctggtatt
    17761 tataatccga tcgatcaccc aaaaaatgcg aatgatcgga aaaacacagt actaagtgta
    17821 atggaagaaa acggaaaaat cacaaaagaa caagaacaag cagaaagcca gacagatatc
    17881 agcgcgtatc ttaatgacac atacgataat tcagattctg gttatcgtta tccttattat
    17941 tttgatgcag tgatcgatga agcagttaac gagtatggtc ttgatgaaga agatgtcatg
    18001 aataaaggct ataaaatcta tacagcttta gatcaggatt atcaaaagca attagaagct
    18061 acttatcaaa acagtgcatt attcccagca aatgcttcag atggtgccat ggtccaatct
    18121 gcttcagtag ctttagatcc gaatacaggt ggtgtacaag cactagtagg acgaagagga
    18181 gatcatgtct ttagaggatt cagctttgct acacagatgc gtcgttcacc aggatcaacg
    18241 atcaaaccat taagcgtata tacaccagca ctggaagctg gatacaagcc ttccagtatc
    18301 ttgaaagacg aaccgcaaag ttattacgat gcgcataatt ttgacgggac ataccaagga
    18361 gaagtaccga tgtatcaagc tgttgcacaa agtttgaatt tgcctactgt atggcttttg
    18421 catgagatcg gtttacaaaa aggatatgat aaagcagaag aattcggcct gcctttagca
    18481 aaatctgata agtattatgg cttagccctt ggtggactgg aaaaaggagt atctccattg
    18541 atcatggcgg gtgcttacag tgcatttgcc aatgatggtc agatgtatac gccgcatctt
    18601 atcacgaaaa tcgttgattc gactggagcg gtgatcgtcg ataacacaaa caaaaaacca
    18661 aaacaggtca tatcaaagga aacagccgat gaaatgacca gcatgcttct tggtacattt
    18721 tcaaatggaa cagcggctgc tgcgaatcct gcaggttata cgatagcagg gaaaacagga
    18781 accacagaaa ctaatttcga tgccacaaaa gcaaatgacc aatggatgat cggttacacg
    18841 ccagacgtag tgatttcgac atggatgggt tacgaagaat ccagcaagct gcactatttg
    18901 gaagggacaa gtggaaatgt tgtcgggaaa gtcttcaaat cagcggcaga aggcatcctt
    18961 ccgtatactt caaaaacagg atttagtgta gcagatgcct atgccacagg aggacaagtg
    19021 gtagctgcag atcaagttgg aaacacagga aattctaatg gagagtctgg aaattggcaa
    19081 gataacttga atcaatatgg tgaacaagca aaagaagggc tgaaaaactt cggcgatatg
    19141 gtgaaagaag gcgttcaggg aattggtgaa gcggctaaag acctctggcg gaaatatcaa
    19201 ggagagtagc ccctttgaaa acgaataacc ccatgttaca atagagagta actactaaaa
    19261 gaaaagaggg cataacatgt caaacatcta cgatagcgcg aatcaaatcg aacgtgagat
    19321 ccgtgaatta ccagaattca aagcgttgaa agaagcgtac gagaaagtac aagcaaacga
    19381 agaagcacac aaactgttca aagacttcca agcgatgcaa atcgaattac aagaaaaaca
    19441 aatgagtgga caagagttca gcgacgaaga tgcaatgaaa gcacaagaaa tggctgtgaa
    19501 aatccaatca gaagaagtaa tcaatgactt gatgcaaaaa gagcaaggct tcagtacaat
    19561 catcaatgac ttgaaccgta tcatcatgac accagttcgc gacctttaca gcgaataaaa
    19621 ctattttatg aaacaacaga gggtgtgaaa gagacgttcg gccttgagcg atgacaaaat
    19681 tgcttcttat tgccgatagg ctgtctcttg aacaccattt accaagaggt cgtgagccta
    19741 ccgtttaact ctgagttaca aggaacaatt tcaaaaaaca gcttctcatg tttgatgact
    19801 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcagaa ataggagcta
    19861 cgacaaaagt cttgtcatag ctcctatttt ttgtcattca tttttcaagc ggtagaaata
    19921 gcctttttga gctaacagtt tttcgtaagt acctatttca acgatttttc cttcatcgat
    19981 cacaacgatt ttatctgcct ctttgatcgt tgaaagccga tgagccacaa taaaagtagt
    20041 gggtgtttct gtcaggtaat tcagggcttg ctggattttt ttctctgact ggttatctaa
    20101 agctgaggtt gcttcatcta gcaaaatgat ttttggctga cgtatcaacg ctcgtgcaat
    20161 ggagatacgt tgcttttggc caccagacag cttgttgcca tgttcgccta ctaaggtatc
    20221 cagaccatca ggaagctggt caatcagtga agaaagttgc gcagcttcga tgacttcatc
    20281 taaacgttct tttgatacgt ttttcaagcc ataagtgatg ttctctttga tcgaagcgga
    20341 gaacaacaaa gtggtttgag gaacaacggc taaataattt cgcacacttc gcatatccag
    20401 ttcatccaaa ggctgatcat ctagaaaaat ttttcctgaa gtcggctgga taaatccaat
    20461 caataagtta aggagtgtag attttccaga gccagatggc ccaacaaaag caattgtttc
    20521 tccagctttc acctctagag aaaaatgatc gataatcggt ttgagtgagt ccggataaag
    20581 aaaagaaaca tcctcaaaac gaaaatttcc atccaaatgt tggatttttt tcttaccgtg
    20641 ataagtttct acatcgccag caatcaacag ttcgccaata gaagtgatag attccatgcc
    20701 ttttgaaatc gtaggaatca gcgtgatcat tccagaaacg gaattgacga tcgtcgtgaa
    20761 atacgtctga tacatcacga catcgccggg ttggatgcgc ccttgtaaag ctaatatccc
    20821 agtgaaaatc aaacaaatca gctgaaatat ctgaaaacta gcccagctga ctgatccgaa
    20881 aacagattgc aggatatcta agtggaatcc tttttcatag atttgagtaa aatgcttatt
    20941 taagcgagtt gtttcgtgtt tttctaaccc atgagctcga gcaatcggtg ctaattcgat
    21001 cacttcgatc acttttgcag aagtctcttc catttcttgc ctgaagtctg agttggactg
    21061 tctgatcctt ttacggaaaa agagagtaac gaatgcggcg agaggaacag tcaataagaa
    21121 aaacatgaaa acaacaggac ttttataaag cgtgatacct aaagctacga ataaattgac
    21181 agcgatattt aatccgctga caaatacttg agaggacaac gtttggatag cttctacatc
    21241 acggatgatc tttgactgga ttcgtccaga ttgcaattct ttttgataag gaatagatag
    21301 ttcttggatt ttcttgataa gggtcgctct tagtccagct tctacttgac ggatcgaagt
    21361 gctatggtat tttacatgaa aataattcgt caatatattt tgaccaatca gacaagccat
    21421 caaaaaagcg ttgaaataaa tggcctgcaa ctgagaagaa tctttcgatg aagctgcatt
    21481 gattacattg gcaatagcga tcggcattaa ccaagatgga agatgtttga taataaaaaa
    21541 gagtcctgac atggcaaaac gccaataatt tccctgataa agatgcagca atgtctgaaa
    21601 agcatttgca tgatattttt gataactttt ctgtattcgt tcctccataa tcttctcctt
    21661 aacaatcttg gtaaataaac tgcaagtgtt ctattgcttc aggaacataa atctcaggag
    21721 tagcttcaag agtggcgtac attagaggac gctcatattt gatataatgc agtaccgggc
    21781 ggaaatctaa acagcccttg ccaacaggta ccatgcggat ctttccattt tccatgatat
    21841 agtctttgag atgaagcgct gtaatataac catgtaaatt gtccaaggca cctcggatca
    21901 ctttttcctg atcagcatga ttttctttat gcatcaaatt cgcacaatct aaaatgattt
    21961 ttaaatttgg tgaagctact tcatcgatta aacgtttagc taactgatag ttatagattg
    22021 gatgattgat ccctgcttca acagcaacag ttacgccaaa attttctgca tgagcgacta
    22081 attcaatgat agaatttctg gcgatctgat aagcttcttc agtaaaattt tcttttgtgt
    22141 aaccgacaga gacactaccg gtttccgtag caatcatctt ggcttgatag gcagagcaca
    22201 gagcaatttg ctgtttgaaa gttgccaatg cttgtgcacg aacttcttga tttttgctgg
    22261 ataggttgat atagcatcct aaaatggaaa gttcgatatt gtttttttgg aagtattcac
    22321 caaaataggc agcactccca agtgtcatgt cagttaattt aagttgttca ggaaatgctt
    22381 tttgtggtgc aaattggata tgggacaagt gaagctgtga tactttttga acgatttctt
    22441 cacgatttcg aacatctaaa tcgtgggcac ggatgcctaa atttaatgga aacatctcaa
    22501 actccttttt gtaaataatt agcaagaggc agacgggatt cttttatcaa ttcaacaatt
    22561 attcgggcaa ctttatcagc gccttctgga ctaaagtggg tattgtcttt tagtccttct
    22621 gggtaatttt tacaagtatc aggtataaga tgcaggtaga atttttttgt ctcttctttt
    22681 gagaaggtct ggaacaattc ttgtgatctt gagaacacat ctaaaagagg aatatggtgt
    22741 ttctcagcaa atgcacgcat cgcttttgga tagtctccaa gtatatctgg attcaatgtt
    22801 ccgttttcta agtaatctcg tcgcgtcaca gatgtcagta agaccatttg gacgttcgct
    22861 ttttttgcgc attcaaaaaa tatctgcaga ttttttaaat agctaccgaa gggaagcgta
    22921 cctctttctt cttggatttt ctggtcgtta tgtccaaatt gtacaaataa gaaatcattc
    22981 ggctgaaact gtgaagctag acgggctaac cttccttcct ggataaatga tttggtgctg
    23041 cgtccattct ttgcttggtt attgatctgg atctcgggtg aaaaataagc agagattttt
    23101 tctccccaac ctgtctcggg acgcttgttt tcatcttttt ctgccgcagt agaatcaccg
    23161 gcaatatgga tagtaggcat gttatctctt ctttcataaa ggtttatagg caatgaagca
    23221 gaacagcaag agaagaagac ggagcaaatt tggtccgtct tcttctctaa aacgtggata
    23281 aacggattcc gttatttcac tatcctgttt ttgattcgtt actgttacta ttaatcaatt
    23341 aggcaaaaaa gccaaagtaa tttttagcat ttgtataact aatcgcttga actagacttc
    23401 ctaaaagttg ttcatcttca ggtgcttctc ctcgcatgac catttcgcct aaaaattcac
    23461 aaagaacacg acggaaatat tcatgtctag tgtaggacag gaaacttctt gaatctgtca
    23521 gcatgccgac aaaattcccc aaagcactac catcagcaaa ttgtgtcaat tgatcgcgca
    23581 taccagctcg agtatcgttg taccaccatc cagaacctaa ctgcagtttt ccattctgtt
    23641 ctttttggaa acagcccatc aaagctaaaa tcgcaggata gtcattcgga ttcaatgaat
    23701 aaaggatcgt ctttggcaac tgatccgttt cttctgcacg gttcaataat ttttgcagag
    23761 gaatagtcaa agaaagatca ttcattccat catagccggt atcgggccct aatttttcca
    23821 acatttgtgt attacagtta cgataagcat ggatgtgcag ctgcatcgtc caattatgag
    23881 aatgatagaa gtgggtcaaa tgtatcagtg tttctgtacg ataagcatcg atttcatctg
    23941 aagataatag tttgttagct cgtgcttttt gaaaaatctc ttctaatact gtttcatccg
    24001 cttctttata gcgtaagatg tctaaagcgt ggtcggaaac acgacatcca tgttgatgga
    24061 agtattctat ccgctgccct aagacttcta tcagttcttg ataggtggtg atcgtttggt
    24121 cagctgcttc ttccagtagt gtgacccatg cattaaatcc ttctttttca atattgagtg
    24181 ctttatctgg acggaatgaa ggtagaacct tcaaatctgg ttcttcatga gcaagcaact
    24241 gatgatagtg caagtcgtct gcgggatcat ctgttgtaca aatcacttct acattcgatt
    24301 tttttgcgat ttcccgacgg cgaaaatcag gagctgccaa aatttcattt gcttgtgtcc
    24361 aaattttctc tgcattttct tcatttatca acaagtcaat accgaagaaa cgcttcaatt
    24421 caagatgtga ccaactgtaa agcggatttc cgataatttt tggtacggtt tgacaccaag
    24481 ctaaaaattt ttcaaaatca gaagcatttc ctgtgatttt ttcttcaggc acgccgcaag
    24541 ctcgcatcaa gcgccatttg taatgatcgc ctcctaacca agcttctgtc agattgctga
    24601 aattgttgtt ttcgtaaatt tcttttggat ctaagtgaca gtgataatca ataatgggca
    24661 tgttttttgc gtaggaatgg taaagctttt tcgcccaatc gtcttttaat aaaaaatcat
    24721 cagataaaaa cataaatctt ccttctttct ttttatactg ttttatgaaa atcaaagtgt
    24781 tactccgttt ttaaaaatgg caatttctcg aacgtcattt tgttcatttt tcgtttcttc
    24841 tccactagct acagcgatga ttttttgaac aaacgattct aagattactt ccattggttg
    24901 ttctaataag acgcctgcat taaaatccat ccaatgacct ttgcgttcaa aaagagttgt
    24961 attcgtagat accttgaccg ttgggacata tgagccaaac ggagtccctc ttcctgtggt
    25021 aaacaagacc aactggcaat cggatgaagc aagagcagag gcagctacaa gatcattccc
    25081 gggagcttgc aataaactaa gccctggttt tctgattttt tcgccatatt gtagcacatc
    25141 aacgacagga gagcttcctg atttttgcgt gcagcctaaa gacttatctt ctaatgtagt
    25201 gatcccacct gctttattac caggagatgg gttttcgtaa actggttccc cgtaagacaa
    25261 gaaatactgt ttgaagtcat tgatcaagtc aacgattgag tcaaaaactt cttcattttc
    25321 cgctcgtgcc atcaatactt gttctgcgcc aaacatttct ggcacttctg ttaagatcgt
    25381 gctgcctcct tgagcaatca agtagtcaga aaatgcacca agtaaaggat tagcagttat
    25441 tcccgataga ccatcagaac cgccgcattt caaaccgaca ttcaacttag agagcgggac
    25501 tgatatgcgc tggtcatttt ccgccgctgt taaaagctct tctaatagag ccacaccttg
    25561 ttcgatctca tcgtaaacat cttgagccac tagaaatttc actcgttctt catcatattc
    25621 acctaaaatt tttttgaatt caggaactgt attgttctca caacctaatc caaatactaa
    25681 aactcctccg gcattgggat gatttactgc gtccgctaaa atttctcgag tattttgatg
    25741 atcttttcca agctgcgaac agccataagg atgctttaaa atcgtaatat ggtcaaactg
    25801 tcccagatct ggatgttttt ccttgaactg tttgacgatc agttcagcaa tgccattcac
    25861 acacccaacg gtaggtacta aaaacaaatc attacgaatc cctacttttc cattttttcg
    25921 caagtaacct tgaaatgttc ggttctcaaa aaggtaatga cttggatgga catctttttc
    25981 atatcgataa tccaattcac cagaaagatt ggtttttaca ttatgagtgt gcagccattg
    26041 accggcagtt acgtctgtcg tcacatgacc gattggatag ccatatttga tcacgttgtc
    26101 tccagctttc aaatcaccca atgcaatctt atgaccttgc gggatatctt caagagtggt
    26161 gatcgtttgc tgatcaatcg tcagttgtgt ttgttttgga ataggggtta atgcgacgac
    26221 cacactgtct cgtcgattta atttcattgt tggttgcagg gtttcaagca tggaccatcg
    26281 ttcctttcat ttcttgtaat actgaccgga cacctttttg atcgatcttt gcagctattt
    26341 cttgaactgt atctgtcaca ttcggtattt gagacaaatc ttctccccaa agcgcagcgt
    26401 tttgcaataa tcgaggaata gctgttccat tatcagacca agcatcagta aattcgctta
    26461 tcgtcttttc atcatcatgt ggctttactt gatcgcctcg atacataaga agtagcgcag
    26521 ctagtgaaac agtgattaac ggtggtacct gattttgttc ttcgatatac ttcttgaaca
    26581 ctggaagcag acgggtgctg aactttgaaa cactatttaa tgaaatcgct tctaatttgt
    26641 gatgggcaaa aggattcaag aaacgttctt tgatttggtc agcataaatg gctaattcat
    26701 ctttaggtaa agaaagcata ggaatcaatt ctagattgaa taactgatca acatatgcgg
    26761 agaataaagg atctttcatt acttcttcta cagtttccaa ttccgccaac aaagcaagag
    26821 ggaccatcgc tgtatgcggt ccattaagca aatgcacctt acgttcacga taaggagtca
    26881 tatcatctgt gatgatgaca ttcaagccag cctgtttgaa tggtagttga ttctccaatt
    26941 ctttaggccc ttcgatcacc caaagcatga atggttctgc tttgaccatc aattgatctt
    27001 catagttatg ccgttgattg aaaacacctg catcttcgcg aggataacct gggacgatgc
    27061 gatcaactaa actacaacag aaaacatttt ctttttctaa ccactcaaca aaagcaggtt
    27121 ctaaatccca tttttctgcg tagcgtaaaa tgatttcttt tagacgttcg ccgtttctgt
    27181 caatcagttc gcaaggaatg atggtaaagc cagctttgtt taatttgaaa cgtcggtata
    27241 aaaaggctgt cagtttgccc gggaagctct gtgggggagt atctgttaaa ttatcagtct
    27301 ccacgaattg gattcctgcc tctgttgtat tggagacgat ataggccaag tcgtcatttt
    27361 ctgctagaga tagataagca tcccattcat cataaggatt gatcatttgt tctactacgg
    27421 tgataatctc ggaagtatca acgatttcgc cattgctgag tccttgtaaa atgaccgtat
    27481 ataaattatc ttgctggttc atcatatttc caagaccttg ttctaaaggt tgaatgattg
    27541 caacattacc tttaaacaat tgttggttat tcagctgctg aatctgccaa tcaataaatc
    27601 cacgcataaa attgccttca ccaaactgaa gaacttttac aggtgcttcg ttttttttca
    27661 ctaattcttt tgtcagtttt ttcatataaa cttcctttct gtgttatgca cacgttaaca
    27721 tttttatata aaattacttg tagtttacaa gtaattgatc gactcacgaa ggatcaacaa
    27781 ggtggagagc tcaattttag attgttcgat cgtcctgcct tcgatcaaat ctagtaaaag
    27841 ctttgttcct tcttgacaca tttctttcaa aggcttacgt acggtggtaa gcggcggatt
    27901 gagataggag acgactggag aatcatcgaa cccgatcaga gaaatgttct ggggaacacg
    27961 gaggccagct aaatgtacag ctctcaatgc accaatcgct atgtcgtcgt tgccgcaaaa
    28021 aaccagagta ggcaggtcag aaagtgataa taggtattcc atgttttgga ggccgctttc
    28081 taagctgtaa tctcctgtta aaaaataggc tggattgaca gggatgctcg ctttttttag
    28141 acttttcaat accccttttt cccgttcttt tgtcgaacga aaatttttct ttccgccaat
    28201 ataagcgatc ttcgtatgtc cgcatccaat accgtaatct actgcctgca taacaccggc
    28261 cgcatcatcg gcagtgacgt tgataaccgc tggatcattg atgtgacgat tgacaacaac
    28321 gaaaggaaat tctttttctt tgagatagtc gatgaaaggc tgatcatcat cactttgact
    28381 cataacgatc agtccatcgt agcgttgcag gttgatttct gttaaatcaa caacatcgat
    28441 actttcaaca gataaactgt aatttgtgtc taatactgaa tggatgcctg caatggaatc
    28501 tgctaggaaa tttgatgaag ttcctttatg caaacttgag aaaaatagtc cgattagata
    28561 ttttttttga ttcactaaac cttttgcact atagtttggc gaataattca tttccttagc
    28621 tagttgtcgt attttttgtt ttgtttcatt ttttattaaa ctgctgttat tcaaagcacg
    28681 agaaactgtc gtatgagaaa cgccagctgc tttagcgata tctttgatcg ttaccattcc
    28741 atcattttcc ttactttttc actttttgtt aacgcattca tcatattacg ataactacga
    28801 taagtcaata gatctttgat atgaaaagat tgcacacgtt aaaaattact tggattttta
    28861 tttttaatta tgctattctt gaaagcgggg aatgctcatt taattagatg agtatcaatt
    28921 ttacagaaaa atgaaagcct tttattttaa aaggaggaga ggaagaaaat gaagaaaagt
    28981 acagtatttt ataaaatatt tttaccgatg tttttattag gagccgtact tgtcatagga
    29041 tttagtttat tcatttacca gaatacctat gaatcgatcg aatcgagtta tttaacagac
    29101 aaaaagaatt tgctcaggca agttaaaact aacgttgaat ggaaaattag aacgattgaa
    29161 tattcgtttt ctacgtatgg ttcgaccaaa aatttctcgg acatattcaa gcatccgctg
    29221 acatatacag attactccac ttattctgaa atcaggaaag aactaaattt tatagaaacg
    29281 attgttatgg atgataataa ttatgattta gtcagtttgg ctggtaaatg gggagtaatc
    29341 aatggatcgt tgagtcaact gaccgaagag gaagttgaaa agtatcaaca gaagtacata
    29401 aataacaaaa ataatttatt ttggcaaaag aaaaacaaag gaatcgaaat ggtgatttct
    29461 ttgccaatgt ttgaaaaaga aaaatatgca ttgggaattg ctacgataga gaaacataca
    29521 atagaacaag ttgttggtga aagtggagaa gacatacttt ctattgaaaa cgaagttggc
    29581 aaccttttta ctactcagac agtaaaaaat aaagagcaac caactgtatt aacggacgtg
    29641 gatctagatt acaatcagct gaaagtgata agagaaaaag ataaaacgta tatagtactg
    29701 aaatcagatt ataataattg gatttacaga ttagaagttg actctagtgc aattggcgca
    29761 atgatccgca atctgagaat cgggctagtt gcagtgtctt taacactggt aggactcatt
    29821 ggtcttcttt cctacttatt ttcggacaga tttgcccgtc cgatttctca aatacaggaa
    29881 cgtctgaatc ttaaatcaaa aggattcagt gggaaagaac tagatttggt cgctcaatca
    29941 gtcagtcgga tcataggaga aaaagaagcg ttaagtgcac acttagttac ccaaaagcca
    30001 cagctggaaa cgctatttgt acttagttta tttagaaatc gagtagaaag gcgagaacta
    30061 gatcagcggt tgcagcaatt tggttatagt tctcaaaatg actgctatta tacagctttg
    30121 gttcaaatcg atctgttaga agatagtcac ggtggggagc gtgatttgct acttttagcg
    30181 atcaacaaca tgatcacgga aattgtgcca aaggaaaatc gaatgctgcc gatcgttttg
    30241 aatgaagaaa tgcaagccac tatttatcga atggataaag atgatgcaga agcaagcaaa
    30301 aaagtcatgg agtattgccg actcattcaa aaacgtgtaa aagaatactt gaatttaact
    30361 atcagtatag gcatcagtaa ccggttcgaa acgctgaatg aaagcaagca atcggttgat
    30421 cgggcaaaag aagccctgta ttatcgtgta aatactggtc caagttcaat tattttttat
    30481 caggaaatcg ttcctgctgc tcatgaaaaa acacttatta gatatccgat agaagaacaa
    30541 aatcgattgt ttgaagccat tcgttctgga aatcaggaag tgaagaattt agtacatgaa
    30601 ttgattgatg cattgtttgc acaaaataaa aatcctttga gtcgggaagt agtttcggtc
    30661 cgcttgatca atgaactcgt tcaattagga cagttattgg gagtagattc aaaaaacttt
    30721 gataacatga aacaaattta tgtaaaagca ctgaacaatt atcatcccaa agaactagaa
    30781 aaacttgttt tagaacaatt agttctgcca atcacagaat gctctcaagc aatcactgat
    30841 catgaattta aaaatttgtc ggagaatatt atgcatatcg tctacacaga atatgatcgt
    30901 gatctgtctt tagatgtgat cgctgatagg ttgcattata accctaacta tttaagtaat
    30961 gtatttaaaa aggaaacagg agaaaaattt ggtgattttg ttcaaaatca tcgtttagag
    31021 gtagcaaaaa aatggttgaa agagactaac ctctccgtaa aagagattgc agaaagattg
    31081 caatatcgaa atccgcaaaa ttttattcgt ttctttaaaa agaaggaatc gattacgccg
    31141 ggagaatatc gaaaaaaata tttgtgaatc aagaggctgt ttttcttgtg gactgattca
    31201 agcaataaaa gaagttagaa taaccctagt atttgtattt atggatagtt aaatttcaag
    31261 gctaagcacg caaaaagaac tgtgacaaaa gtcttgtcac agttcttttt tacgaataaa
    31321 tggcgttcaa gagacaggct atcgacaata aggcataatt ttgtgaaata tgggaagcta
    31381 tttttcaaaa tcgcttctta ttgctcctgt gggttactcg tcgcaatgtt tgtgagagtt
    31441 tttatcatcg ctcaaggctg aacgtctctt tcacaacctc tgatacacgg tgttccagga
    31501 gtagattctt cgaaaaataa gccgaaattt cacaaaaatt taagaagcaa ttttcgtaaa
    31561 tgccttctta tttcttgaag ctgaccactt ctgacacaac taccattttg cgttcagtac
    31621 ataatcaccc tttgacagaa cctacgagtg ctcctttgac aaaatatttc tgaacaaaag
    31681 gataaacaat cagcataggc actgttgcaa taacgataac agccatttta actgtttgtg
    31741 ctggtggaat cacatcaacg gcactacttt ctgcttgcat cccacttgat acaatgacga
    31801 tttggcgtaa aagaatctgt atcggccatt tagatgaatc gttgatatac aaaatagcag
    31861 tcatataagt gttccagtaa gtaacagcat aaaaaagcga gatagtagca atagacggta
    31921 acgctaatgg cagcataatt ttgaaaaata caccaaaatc tgtgcaacca tccatttttg
    31981 ctgattcttc caaactatcc ggcaaggctt ggaaaaagtt tctcataatg atcatattca
    32041 ttgcattaac agccacaggc aggatcatgg cccagtaaga attgaccagc cctagagaac
    32101 gaacaactaa gaacgttgga atcatccccc cgctgaacaa catagaaaat acaacaagaa
    32161 agttgacgaa acctcgacca aacaaatact ttctggaaag accgtaggcc atcagtgaag
    32221 ttgcacacat actgacgatc gttccgacac ctgtaacacc aatcgaaaca gcaagtgctc
    32281 taaaaatggt cggcgtagaa agaatgtagc gatacgcgtc taaagaaaag gtagtaggaa
    32341 aaaggataaa ctttttcgcg acgacttctt gtgtagaagc aaaagatgta gcgatgatat
    32401 ttaaaaaagg aacgatacag atcaaagcta gcaaaattag aaacgtattg ttgaaaagtg
    32461 taaaacttct ttctcctata gagactttct ttttcatata gataggctcc tcctcaaaat
    32521 aaattaaatg tttctgtttc tatatgttca atttatcatc ggtgaaagat tacgtatata
    32581 atcatttcct atgctaagtg tcagatagaa aaaaatagaa ggtttgctat gcgtagttta
    32641 tttgtgaaaa aatacacttt atcgttttgt atagcacaaa aatcattgct atgtaagcgt
    32701 ttttacttac ggtaaaaatg attatatacg taattcagat agttatctat catgaagtca
    32761 taagcagcaa acattcacaa aactaaggag gggcttatgg aaagtgtaaa gaaacaagaa
    32821 ccatttcaac tgacgctatc gaaaaaagaa gaaaagctca gaagacgaaa aaaaataatt
    32881 gcaggaatca aaacgaataa atttttatat gtgatgattc tgccgggagt cctttatttc
    32941 ttgattttta ggtatcttcc tatgggggga ctcgttattg cttttcaaga ttatcaacca
    33001 tttttaggaa taatgggaag tccttgggtc ggtttcaaac atttcattcg tttgtttaca
    33061 gaaccgacat tctttacatt attaagaaat acgttgatct tgttcggatt aaatatcttc
    33121 gtgttttttc cgatgccaat cattttgtct ttactattaa atgaagtaac gaacaaacga
    33181 ttgaaaaacg ggattcaaac gatcatttac atccctcact tcatgtcttg ggtaatcatt
    33241 gtttcgatta cttatgtatt tttgaatgtt gacggagggg tattgaatga attccttgca
    33301 acaatgggat ttgaaaaaat cagttttcta acatcccctg aatggacacg cacagtatat
    33361 atcggacaag tgatctggaa agagttgggc tggtctacga tcatctacct ttcagcgata
    33421 actgcagtcg atacacagtt gtatgaagct gcagaaatgg atggagcagg acgcttacgc
    33481 aaaacatggc atgttacatt gccggcaatt cgtccagtaa tcattacctt gctgattcta
    33541 aaaatcggtc agacattaga cttagggttc gagcatatgt acttgttatt gaattcattg
    33601 aatcgaagcg ttgcagaaat tttcgacacc tatatttata cagctggatt gaagaatgga
    33661 cagttaagtt tcagtacagc aatgggattg tttaaagggg tcgtaggatt gtttatggta
    33721 attttagcta ataagattgc caaaaagcta ggagaagacg gcgtttacta agatagaaag
    33781 agagggaaag atatgctgca gtcattagat aaaataaacg gaaggctaaa tcgtatttta
    33841 cagttggttt atttgaattt tctgtggggc ggtttagtca tcgcaggttt gggagttttt
    33901 actttcggtc ctgcatcgta tgcgatggtt gcagtgatta gaaaatggct gcgctcaaaa
    33961 gaaacgtttc ctttgacgaa aacgtacttc acatatttta aagaaaacta tcgtgaaacc
    34021 ttgctgatga gctggatata tggcgccatc gggattgttt tacttgtgga tctttctttc
    34081 atacacaatt ggtatcttag gatcgctttg tatattgctg cgttcatata tgtgctctca
    34141 gcactctata ttttcccaat catggctcat tatgattgga aaggatattt tttcaaaatg
    34201 aaaatggcat ttttattcgg attctcacat cttcattact cattagtgtt gatattaagt
    34261 atggtaggga tttatctgat gatcatccaa acaatgccag gtgttttaac ctttatcggt
    34321 ggcagtctct tcttttccgc agtcacgtgg acagcaaatt ctatattttg ccatttggaa
    34381 tggaagaact catatacaga aaaaacagaa gaaaacaaaa atattattaa aacagtgaag
    34441 gagaaaattt atgaaaaaag caacgctcgt aaaattaggt cttagttttg caggaattct
    34501 agcacttgcc gcatgcggca gcagttcaag taacagttct tcagagacag aagatggaaa
    34561 agctaaaatc acttggttaa atattttgca tcatgcttca ccgcctacag acacgattgt
    34621 gaacttgatc gaagaaaaaa ccgatacgga actgacattc aactggattc ctgatgcttc
    34681 aaaagaagaa agattaacaa ctgcattggc ttctgatgaa ctaggagata tcgttacact
    34741 tcaaattgcg atgttgaaaa actcttctgt ccgcaatgcg ttaaaatcag gcgtattctg
    34801 ggatgtatca gaatatttaa aagactataa aaatttaagt caaatttctg aagatagaat
    34861 cgaagctgcg agtatcgacg gtgccttgta cggcgtaccg atacaaaaag actacgctcg
    34921 tgctggctta gtcattcgaa aagactggtt ggataatcta ggactagatg ttccaactac
    34981 catggatgag ctttatgaag tagctaaaca atttacagaa aacgatcctg atggaaatgg
    35041 tgtggacgac acagttggtt ttggcgatcg tgcgcctagt gatattcgtt ataccagctt
    35101 taaacttttc acttcttatt ttggagcacc aaatggctgg gctgtagacg ataatggcaa
    35161 attcactcca gaatttgata cagaagagta tatgaaagct ttgaattttt caagagatct
    35221 atttgagaat ggctatttgg cacaagactt tgcagttacc caaaagactg atcagcaaga
    35281 acaatttgca caaggaagaa ctggaatcta cactggaatg gtggatatca agaatctgaa
    35341 gacgatgggt caaggtattc aagatgatat ggaattagtg cctgtaaaca agatctcaga
    35401 tggtgaaggc ggcggttatc atgtttggtc agaaggaaat ggtattggcg gcttgctagc
    35461 atttcccaaa tctcaagtaa aagacgaagc acaattgaag aaattacttc aatttgttga
    35521 tgatctttta gaagaagatg tgtataaatt gatgactggc ggtatcgaag gaacgcatta
    35581 tgaattgaat gatgatggtt ctatcgaatt tatcgatatg gatctttggc aacaagaggt
    35641 gcaaccgtta tcaagctctc gtcctagtga agtgacgttc tctttcaaag atacagaccc
    35701 tgaaaaagaa ttctctaatc aattgatcaa agaaaatgaa gaatttgctg taatggatcc
    35761 ttctgtgcca ttggattcac cgacgaacaa tgaagtagga tcagaaattg aaaaaatcgt
    35821 cattgatgct acggtgcaat acatcatggg acagcttgac gaagatggat tcaaaaaagc
    35881 tgtagaagac tggaaagcac aaggaggcga tcgaatcaca caagagtacg aggaagcata
    35941 caaagcagcg gcacagtaat atagacgatt gggacaaata cttcttggta tttgtcccaa
    36001 atcctatttt aatttttaaa ggagagcaaa taatggaaaa ctggtcaaag aaattagcaa
    36061 aaagtatcat ggaaagaaca cctcgcttat atgaagaaaa gtggtataaa ggaaaatggt
    36121 cttatgacta tggcgtcgta cttaaagggt tccaattact ttgggaacga acccaagaaa
    36181 aaatttattt tgatttcata aaagacaata tcgactattt cgtgcaagaa gatggaacga
    36241 tccgtggcta cagcgttgaa gaatacaata tcgaccatgt aaatacagga aagcttttct
    36301 ttttattgta caaagaaaca ggcgaagaaa aatataaaaa agcagcagaa ctcctttccc
    36361 agcagttggc aaaccatcca cgcacatccg aaggagcttt ttggcataaa gaaatttatc
    36421 cttatcaaat ttggttggat ggcttatata tgggttctcc tttttatgct gaatttatcc
    36481 agaattttag tgaagaaaaa gattaccaag atgttttgag acagtttgaa atcagttata
    36541 aacatctatt cgatccaaaa acaaatttgc tgattcatgc atgggatgaa aagaaggtcc
    36601 aaccatgggc tgatccaaaa acaggtctat cccatcattt ttggagccga tcgattggct
    36661 ggtatttgat ggctgcagca gatacctatg aattacttca tgaagaaaca gattgttctt
    36721 tgctgaaaga aattctagaa aagacactgg ctgcattact tccatatcaa gaagaaaaca
    36781 gcgggacatg gtatcaagta acaacagaaa caaaacggaa aggaaattat ctggaagctt
    36841 ccggcagtag tatgtttgtt tacgcaatgg ctaaaggaat acgtttggga ctgctgaaca
    36901 aagaagaatg gcttccacaa gtgaagaaat cacatcaagg attacttgat gaatttgttc
    36961 tggaaacaaa agaaggctgg ctgaacttga ataaaaactg tcaagtagca gggttaggcg
    37021 gagcagatca gcgagatggt tcatatgctt actacatcag tgaaccaatt atttgtaatg
    37081 atcaaaaagg cgttggtgca ttcttgcaag cattgattga agtggaggca ttataaatat
    37141 gtatgatatt ttgacatttg gcgcatcaat tgatgaattg aatactgaag caatacaaca
    37201 agccattgat gctgcagcaa gtgacggagg cgggaccgta gttgttccag caggtgaatt
    37261 tctaacagga gctttatttt taaaaagcaa tgtcgaactt catttaagcg caggagcggt
    37321 tctgaaattc tctgacgatc caaaagatta tcctgttgtt cattcacgtt gggaaggagt
    37381 ccatcgaaaa gtttatgctt cctgtatcta tgctcaaaat gtagagaaca tctctgtcac
    37441 tggtttcggt acattagatg gaaatggaaa aaaatggtgg catactttta gaaatgaacc
    37501 agataatctc gcttatcctc gtcctaaatt gatgagtttc cataactgtc atcgaattac
    37561 tgtgaaagat attaaattga tccagtcacc aagttggacg atcaacccga ttctttgcag
    37621 taatgcaacg tttgataact tgacgatctt gaacccagcg gattcaccaa atacagacgg
    37681 aattgatcca gaatcatgca aaaatgtgcg tattagtaat tgccatattg acgtgggaga
    37741 tgactgtatt gcaatcaagg cagggactga agatacgtac gaaaggatcg cttgcgaaaa
    37801 tatcacgatc acaaattgca cgatggtcca tggacatggc ggtgtcgtat taggaagtga
    37861 aatgagtggt agtattcgta atattaccat ttcaaattgt atcttccaag aaactgatcg
    37921 aggaatacga ttgaaatcta gacgaggtcg aggcgggatc gttgaagata ttcgagtatc
    37981 caatatagtc atggataatg tgatgtgtcc atttatactg aatctttatt atttctgtgg
    38041 tccgcgaggg aaagaaccgt atgtatggga aaaaaaggct tatccgatag atgaacggac
    38101 accagcattc agaaggatcc atttttcgaa tatcacggca aggaatgtcc atgcttctgc
    38161 aggttttatt tatggattag cggaacaatt catacaagaa atcacatttg acaatatcga
    38221 tgtctcgatg gcccaagatg cagttcccgg tgttcctgct atgatgacag gaatagagga
    38281 aatgagcaga caaggatttt atattggctt tgcgggtaag gtttccttta accagatgtc
    38341 gattgaaaat cacgagggac cagcatttct attagaaaat actaaagaaa tcagtattga
    38401 gaaattccaa tcaaatcaat cagaaaatat ggatgaactg gttgttcgta aaaactttgc
    38461 ttatgcagtt gaatgatttt ttaggtgctc catctagcag acatacccct agtggaaaac
    38521 gattataccg agaaaaacaa aacggtttta tgcttgaata ctatcaattg tacttaaatg
    38581 aattggaaac agtaccagcc ctctatgctt atccagaaaa agaaggaccg tttccgccgg
    38641 tcctttacct gcattctcac ggcggagatt tttcagtcgg gaaaagtgaa ttgatccatg
    38701 gtgctcctta cttagcttct ccatcttttg cagaagtact gacaggaatg ggatatgctg
    38761 tttggtcgat tgatgcttgg ggatttgaag aacgcggagg tatttctgaa agcgagttgt
    38821 tcaaacaatt tttattaaca ggaaaaacgt tatggggaat gcgtatttat gatgtgatca
    38881 gcttgatcga ttatttggag acaagggaag acacagatac tcaacggctc gcaacgattg
    38941 gtctttctat gggaggctta ttaagctggt gggtgacagc attggattct cgtgtcaaag
    39001 tttgtattga tctagcggca caggtggaca tcgaaatact tttgctacat cgccgcctag
    39061 atcatcatgg gttttattac tatgtgccaa atctattgac agcttataca acgttagcaa
    39121 tccaagaaaa aatcgctccg cgccctcgtc ttagcttagt tggaaaaaac gatacgatgt
    39181 gcttggacga aggcgtactg aagctgagaa aagggctgga caaaaagtat caatcaatgg
    39241 gtagtccaga aaacttttct tctttttctt taacaggcgg tcatatggaa acacaagaaa
    39301 tgagaaatat ttggaaacaa ttcataaggg agcatttatg aggatgaaag acaaactgat
    39361 aattggaaag aatgctatag ataccccgac ggattttgaa actatccaag aaggaatcga
    39421 ttatttggca ggcctgccgg atagccatcc aaaggtcttg atagtaaaag aaggaagata
    39481 tgatgaatat atagaaagca gattatccaa ttttaaactt gtgggagcag gaacagtagt
    39541 tatctccggt aatcgttttg caaaacagga gtatcttgac ggtagccagt tagggacttt
    39601 ccgaagtgct acttttttct tagaaggatc gaatgtcgta ttagaaaatc ttcacatcga
    39661 aaatacagca ggtccaggag aaaaggtagg acaggctgtt gctttattca attcaggaga
    39721 tcgcgtatct ttaaaaaact gtcgactatc tggttatcag gataccttat gcacgggtcc
    39781 gttgcctgat ttgcaaaaag atggtactcc tttttgtaca ccggcttctg ccaatcctga
    39841 gtattgtcgt cagacctatg aatattgctg gattgaagga acggtggatt tcatatttgg
    39901 tggggcagat gccgtttttc atcagtgtca tatccatagc ttagccagta aaaatcctgg
    39961 atacatcaca gcagcatcta cgccgaaaaa tcaaaaaaca gggtatcgtt ttgaaagctg
    40021 taccttgact gcagaacctg atactagaaa cgtctattta ggaagaccgt ggcgtccgta
    40081 tgcaaatgtc ttatttgaag cgtgtaagat aggtgggcat gttcacccaa ctggttggga
    40141 taattgggat gacaagaaaa atgaacagac tgtcaaattc tctgaacgta ataacaatta
    40201 tgaaacatcg atcaaaagac ctgaatggat acatttagaa aagcaggggg ttcagaaatg
    40261 aaaagaaaat gggaactatt gctaggaatg gtcggtggca gtctatcttt gattttcttt
    40321 ggcgggttag cagttacttt aagtaatatg tctgcgtcag agtttaaaaa aagttatcag
    40381 tcgcttgctg tagatcattc aactttgtct ttagaaaata catttggatt attacaggac
    40441 atgactggtc tgtttgccgt tgtgttgttt atttcacttg cttttttagc agtagcattg
    40501 tttttgactg ctaaaggaaa atacttaaca actgccactg gtttgtattt tataacagga
    40561 ttcatattgt tgattggcac tcagtttatt gcgtttcctt ttgcattttt ttatttcgca
    40621 gctggggcat tcagtttata tcgagtaaga atgagaaaag gagcctgaat catgcagact
    40681 atcaaagtga aaaatcctat tttaagagga ttcaatccag atcctaatat attaagagtg
    40741 aaagagcggt attacattat tgtctcctct tttgaatggc ttccaggaat ccgagtatat
    40801 gagtcgaaaa atttagtcaa ctggtcacat acaacggata ttttgaccac tcaagtatct
    40861 ttgcaaggaa atcctacaaa tggaagtata tgggcgccac aaatcagtta tgctgatgga
    40921 ttgttttatc ttgtttacac agatgtcaaa agtgcaggcc gtccgtttaa agatgtccac
    40981 aattatttga tcactgcatc tgagatcaca ggtccttggt ccgaaccaat ttatttaaat
    41041 agtaacggct ttgatccttc tttgttccat gatcttgaaa cgggaaaaaa atggcttgtc
    41101 aatgaactat gggattatcg tctggacaca ccgaataaat ccagcggtat cgtattacaa
    41161 gaatatgatg cagagaagaa gaagcttgat ggtcccatct ataaaatatt tgcaggtaca
    41221 gaattagcga aaacggaggc accacatttg tattttcgta acgggtatta ttatttgatg
    41281 acggcagaag gcggtacagg aagaggacat tcagttacag tttgccgagc aaagaatatt
    41341 actggtcctt acgagcttga tcctggtttt cctatgctga cagctagtga caaacctgct
    41401 tctacattgc aatgtacagg acatgggagc ttagtacaaa ccccgtcagg aagatggtac
    41461 atgacatatc tttgtaccag accattaaac ggtgcggcta ttttgggaag agaaaccgct
    41521 attcaagaag tgtattggac gtccgacgat tggttaagac ttaaagatag ccagacaact
    41581 ccgatggaag aaataatgat tgagacaaat gaagaggtac aacaagaaaa agatcatcag
    41641 tttatggata catttgaagg agagctaaag aagaactgga atgccagacg cattttgcca
    41701 aatgctgatt ggtgtgatac aaaatgtaga ccaggttttt tacgtctgat ttccggtgaa
    41761 tctcttcaat ctaccttcga acatcatttg ttagctattc ggcaaacgga ttttactttt
    41821 gatgcggaga cagcatttga atacatgcct aataatttta atcagatggc tggactggta
    41881 ctgtacttaa atgaatccaa ttatatttat tgttatgtta cttggaatga ggacaaggga
    41941 aaatgcctac gtatgataga atcagaaaac ggagtagcac aaatagaaga ttggacgatt
    42001 ccactcacag aaaagaagac ttttttgaaa gtcactgtaa agaaagagac agcacagttt
    42061 catttgaaag acgtatcaac tgaagaatgg aagaaagtag gtccgcaaaa aaacatgtgt
    42121 atactctcag gagggtttac tggaaatttt atcgggatag cagtgcatga tctaaataaa
    42181 aaaagaggaa gttatgctga ttttgagttt ttcaagtatg atggaaaaga tcatctgtaa
    42241 tttaattctt taataaatga ggttcactaa cttattttca ctagttagtg aatttttttg
    42301 tttgtatata ttgttatgta aaaaaagtta tcaatcttat gcatcaatgt ttttagatta
    42361 ttttttgttt tttatctgtc aagataatgc aataaaaagc caacattgtg aatataaaaa
    42421 caagaaaaaa agagttataa ttttgaaaac gtttactaga agaggtagga ggaattcaaa
    42481 tgctttcaca aaatgctgtt acagcaaata caataaaaaa agaaaaaaag gaatttggga
    42541 tttccgataa gataggctac atgttcggtg atctaggtaa ctgttttatt ttaggtttag
    42601 tcaatagttt cttgatgatt tattatacga atgttcttgg cgtttcaggg gcaatcgtag
    42661 gtacgttatt cttgatttcg agaattttgg atgcgtttgc tgatgtgatg gttggaagat
    42721 tatgtgatgt ctcagagtta acagaaactg gccgatttat tccttggatc agaaaaatga
    42781 agtatccatt ttgcttagtg actgtcattt tgtttttgcc attcgtccaa catcttccaa
    42841 tgacggtgaa gattgcttat atttttgttt catacattgt ttatgggatt tttctctcaa
    42901 caatcaatat cccgtatgga gcaatggcag cagcaattag ctcaaatcct aatgaccgca
    42961 cctcgttgtc cacgttccga agcattggtt ctgcgattgg cggatcaact acaggattct
    43021 tcattccgat tttaatgtat acccataaca agagtggaca acaagtcgtt tcagggaatc
    43081 atttcttttg gatttcaatc ggatgtgctg caatagcatt tgtcgcatat atgttaactt
    43141 gtaaattgac tactgaaaga gtaagagttg tcaaaaacga aaaagtatct gcttctcagt
    43201 tggtaaaagg tttgttagct aatcgagcac tgattatttt agtcattgtt gacattttca
    43261 ttgtgatcaa tcagatttta gctgggacga atatgactta tttattcaat gattacttcc
    43321 ataataaaca agcaatgtct gttgctcttt tatttactta cggaacagta gtagttttag
    43381 caccatttgc tacaaagctg acaaaattat ttggtaaaaa ggaagcaagc gtatttgcgt
    43441 tacttgcttc ttcatgtatg tacttgctga tgtatttcct tcatataaca aatgcatggg
    43501 tatatctgat tcttttattt atcgctactt taggatctgg tttattcaat ttgatggtct
    43561 gggcattcat tactgacgtg attgattatc atcaatataa gacaggctta agagaagatg
    43621 gaacagtata tggagtaaat tcttttgcga gaaaagtagg acaagcatgt gcaggagcgg
    43681 taggcggatt tatgctggca ctcattggtt accaatcgtc tacaacaggt ggagccatcc
    43741 aatcagtttt agtacaagaa aggatttata cgatggcaaa cttgttacca gcattctgct
    43801 tatttgtatc cgcaatcatt ttgctagtag gttacccact gaatcgttca aaaactgtat
    43861 ttattggtga agaattgaaa aaaatcaatg aaagataggg agaaacgtat tgcggacaaa
    43921 aaaagggatt atcaatgctt tgaactttaa aacaaacgac actcaacaag atgatacaat
    43981 aatactgcaa aaagcaatcg atcaaggtgc gaaagaacac ttacctgtat ttattccaaa
    44041 aggcatttat ttagtcggag cccttttttt aaaagataag agtcatctta tctttgaaga
    44101 aggtgcagtc ctcaagggga gaacagatat cgaagcattt ccagaaatcg atacacgagt
    44161 agccggcgta gaaatgaaat ggccagcagc gattttgaat gttctctcgg cgaaagatat
    44221 tcttatagaa ggaaaaggaa taatcgacgg tcaaggagac cattggtggg agctgtattg
    44281 gggaaaagat caaaaaagcg ggacaagagc tgaatacgat caaaagggac ttcgttggat
    44341 tgcggattat gctattaaac gtccacgcgc ttgtttactg tatcatgcag aacatgttgt
    44401 tgttcgtgat ttgatttttc agaaatcagg tttttggaac ttgcaaatca cttattctaa
    44461 cgatgtatta gtagaaaaag taatcatccg caacaatgat ggaccaagta cagatgggat
    44521 tgatattgat tcttcaacaa atgtgcgtgt ctatgaatgt gatttggcct gtggtgatga
    44581 ttgtattgct attaagtctg gcagagatgg caatggtgca agagtcaatc gaaaatcatc
    44641 aagaatcgag gttgctagat gtaaaatccg ttcaggatac ggtgtaacta ttggtagcga
    44701 agttagtgct ggagttagtg acgtatatat tcacgatatc gatttttttc agtcagactg
    44761 tggtattcga atgaaatctt caaaagaaag agggggagtg atcgaaaata tcagggtaga
    44821 aaatctgaat atgattgatg tacaatttcc tttttcttgg ataatggact ggcataatga
    44881 atacaataga aaaaatagca atgaccttgc tcaaatgcca gaaagttggc aagcagtggc
    44941 tgaagaaata cctgaatcaa agcaaatgac gaaagtcaaa gacatacaca tcaaaaacgt
    45001 acaggcatgc ctttctccag gttactcact tccttcccga gcttttgatt tagtagcatt
    45061 tcctgaaaag cctatagaag atgtctgctt ttctcattgc caaataactg caaaagaatt
    45121 cgggcgaatc gaagcagtac aaaacttatg tttcaaaagc tgtgatttat ctatagaaac
    45181 tggcaacaca gctgcaaaca atacttttga caatcgttga aattataata aggtcaagct
    45241 taaataatat tttagcttga cctttatttt ttttaatgat atattaaaca acgttgtcgc
    45301 gcttcattga aacattttat gaaaattcca aaaaagagtt gacacatgtt tttagacgtg
    45361 ataagataac taagtcgtta aaaagaacca cttgcgaagc ttgattctat cgaaaaaaac
    45421 ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg ataaatgagt tgctgaaaga
    45481 tttcgaaaaa cgaaatcgaa aaaaactttt aaaaaagagt tgacaatcgt ttgtctctct
    45541 gatatgatat aaaagttgct acggcagcca aaaatgaatc gggaaacact ctcgatgaag
    45601 tagacctttg aaaactgaac aaagtaagac aaaccaaatg tgtagggcgt cttgattcaa
    45661 ttcaagacaa
//
LOCUS       DAYYAJ010000008        39650 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000008 DAYYAJ010000000
VERSION     DAYYAJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.008"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..574)
                     /locus_tag="ACHATZ_000391"
     CDS             complement(29..574)
                     /locus_tag="ACHATZ_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8402952.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACHATZ_000392"
     CDS             complement(586..1278)
                     /locus_tag="ACHATZ_000392"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8402953.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHATZ_000393"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHATZ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8402954.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACHATZ_000394"
     CDS             complement(1594..2157)
                     /locus_tag="ACHATZ_000394"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8402955.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACHATZ_000395"
     CDS             2385..3047
                     /locus_tag="ACHATZ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8402956.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACHATZ_000396"
     CDS             3171..4367
                     /locus_tag="ACHATZ_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8402957.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACHATZ_000397"
     CDS             complement(4468..5844)
                     /locus_tag="ACHATZ_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8402958.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6748)
                     /locus_tag="ACHATZ_000398"
                     /pseudo
     CDS             complement(5881..6748)
                     /locus_tag="ACHATZ_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6837..7862)
                     /locus_tag="ACHATZ_000399"
     CDS             complement(6837..7862)
                     /locus_tag="ACHATZ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8402959.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7880..8554)
                     /locus_tag="ACHATZ_000400"
     CDS             complement(7880..8554)
                     /locus_tag="ACHATZ_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8402960.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8738..9631)
                     /locus_tag="ACHATZ_000401"
     CDS             complement(8738..9631)
                     /locus_tag="ACHATZ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8402961.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9705..11402)
                     /locus_tag="ACHATZ_000402"
     CDS             complement(9705..11402)
                     /locus_tag="ACHATZ_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8402962.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11395..12708)
                     /locus_tag="ACHATZ_000403"
     CDS             complement(11395..12708)
                     /locus_tag="ACHATZ_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8402963.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12708..16649)
                     /locus_tag="ACHATZ_000404"
     CDS             complement(12708..16649)
                     /locus_tag="ACHATZ_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8402964.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16758..17216)
                     /locus_tag="ACHATZ_000405"
     CDS             complement(16758..17216)
                     /locus_tag="ACHATZ_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8402965.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17514..18032)
                     /locus_tag="ACHATZ_000406"
     CDS             complement(17514..18032)
                     /locus_tag="ACHATZ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8402966.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18034..18651)
                     /locus_tag="ACHATZ_000407"
     CDS             complement(18034..18651)
                     /locus_tag="ACHATZ_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8402967.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18648..19502)
                     /locus_tag="ACHATZ_000408"
     CDS             complement(18648..19502)
                     /locus_tag="ACHATZ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8402968.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19499..20410)
                     /locus_tag="ACHATZ_000409"
     CDS             complement(19499..20410)
                     /locus_tag="ACHATZ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8402969.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20451..21785)
                     /locus_tag="ACHATZ_000410"
     CDS             complement(20451..21785)
                     /locus_tag="ACHATZ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8402970.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21993..22823
                     /locus_tag="ACHATZ_000411"
     CDS             21993..22823
                     /locus_tag="ACHATZ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8402971.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23067..23316)
                     /locus_tag="ACHATZ_000412"
                     /pseudo
     CDS             complement(23067..23316)
                     /locus_tag="ACHATZ_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23507..23701)
                     /locus_tag="ACHATZ_000413"
     CDS             complement(23507..23701)
                     /locus_tag="ACHATZ_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402972.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24206..24391)
                     /locus_tag="ACHATZ_000414"
     CDS             complement(24206..24391)
                     /locus_tag="ACHATZ_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402973.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25241..25582)
                     /locus_tag="ACHATZ_000415"
     CDS             complement(25241..25582)
                     /locus_tag="ACHATZ_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8402974.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25582..25812)
                     /locus_tag="ACHATZ_000416"
     CDS             complement(25582..25812)
                     /locus_tag="ACHATZ_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8402975.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26099..26437
                     /locus_tag="ACHATZ_000417"
     CDS             26099..26437
                     /locus_tag="ACHATZ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402976.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26642..27204)
                     /locus_tag="ACHATZ_000418"
                     /pseudo
     CDS             complement(26642..27204)
                     /locus_tag="ACHATZ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27936..28517)
                     /locus_tag="ACHATZ_000419"
     CDS             complement(27936..28517)
                     /locus_tag="ACHATZ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8402977.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28700..29326)
                     /locus_tag="ACHATZ_000420"
     CDS             complement(28700..29326)
                     /locus_tag="ACHATZ_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8402978.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29348..30679)
                     /locus_tag="ACHATZ_000421"
     CDS             complement(29348..30679)
                     /locus_tag="ACHATZ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8402979.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30834..31235)
                     /locus_tag="ACHATZ_000422"
     CDS             complement(30834..31235)
                     /locus_tag="ACHATZ_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8402980.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31321..31527)
                     /locus_tag="ACHATZ_000423"
     CDS             complement(31321..31527)
                     /locus_tag="ACHATZ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402981.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31622..33124)
                     /locus_tag="ACHATZ_000424"
     CDS             complement(31622..33124)
                     /locus_tag="ACHATZ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8402982.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33142..33597)
                     /gene="argR"
                     /locus_tag="ACHATZ_000425"
     CDS             complement(33142..33597)
                     /gene="argR"
                     /locus_tag="ACHATZ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8402983.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33695..34312)
                     /locus_tag="ACHATZ_000426"
     CDS             complement(33695..34312)
                     /locus_tag="ACHATZ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8402984.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34533..35906
                     /locus_tag="ACHATZ_000427"
     CDS             34533..35906
                     /locus_tag="ACHATZ_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8402985.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35981..37093
                     /locus_tag="ACHATZ_000428"
     CDS             35981..37093
                     /locus_tag="ACHATZ_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8402986.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37165..38802)
                     /locus_tag="ACHATZ_000429"
     CDS             complement(37165..38802)
                     /locus_tag="ACHATZ_000429"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8402987.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38970..39578)
                     /locus_tag="ACHATZ_000430"
     CDS             complement(38970..39578)
                     /locus_tag="ACHATZ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8402988.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11382 a         8437 c         6887 g        12944 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaaaatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggga taccacctaa aatgactaaa aggcaaccaa
     5941 caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta
     6001 aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt
     6061 tgactgttag aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt
     6121 tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca
     6181 ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga
     6241 tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat
     6301 caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg
     6361 caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc
     6421 attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca
     6481 atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa
     6541 cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac
     6601 taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt
     6661 gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca
     6721 tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa
     6781 acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa
     6841 cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct
     6901 tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa
     6961 agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta
     7021 tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg
     7081 ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat
     7141 ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa
     7201 acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc
     7261 tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc
     7321 cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt
     7381 gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga
     7441 actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa
     7501 tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc
     7561 agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa
     7621 ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc
     7681 aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca
     7741 attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca
     7801 ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc
     7861 atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat
     7921 atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg
     7981 tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta
     8041 agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat
     8101 caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt
     8161 ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga
     8221 tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt
     8281 tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt
     8341 tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat
     8401 ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg
     8461 tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga
     8521 tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg
     8581 ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt
     8641 tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca
     8701 gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt
     8761 gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata
     8821 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
     8881 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
     8941 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
     9001 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
     9061 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
     9121 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
     9181 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
     9241 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
     9301 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
     9361 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
     9421 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
     9481 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
     9541 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
     9601 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
     9661 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
     9721 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
     9781 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
     9841 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
     9901 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
     9961 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
    10021 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
    10081 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
    10141 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
    10201 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
    10261 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
    10321 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
    10381 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
    10441 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
    10501 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
    10561 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
    10621 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
    10681 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
    10741 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
    10801 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
    10861 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
    10921 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
    10981 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
    11041 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
    11101 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
    11161 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
    11221 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
    11281 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
    11341 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
    11401 atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc
    11461 tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga
    11521 ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt
    11581 ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc
    11641 taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac
    11701 ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc
    11761 cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt
    11821 aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt
    11881 gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata
    11941 tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt
    12001 ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac
    12061 atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat
    12121 tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc
    12181 tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg
    12241 tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac
    12301 cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt
    12361 cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc
    12421 atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg
    12481 tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg
    12541 gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc
    12601 cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag
    12661 cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc
    12721 tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat
    12781 gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca
    12841 ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct
    12901 aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact
    12961 tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct
    13021 cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc
    13081 acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg
    13141 tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc
    13201 tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt
    13261 tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt
    13321 tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac
    13381 ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt
    13441 gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt
    13501 aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa
    13561 cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa
    13621 gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct
    13681 aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat
    13741 aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc
    13801 ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca
    13861 tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg
    13921 gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact
    13981 ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc
    14041 cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt
    14101 tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta
    14161 ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc
    14221 agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa
    14281 agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact
    14341 ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg
    14401 gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt
    14461 ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc
    14521 ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt
    14581 tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc
    14641 tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg
    14701 gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc
    14761 tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt
    14821 tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta
    14881 taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct
    14941 tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct
    15001 ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta
    15061 aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata
    15121 tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg
    15181 ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt
    15241 tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc
    15301 tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt
    15361 tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga
    15421 gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct
    15481 tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta
    15541 ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct
    15601 tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc
    15661 ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt
    15721 ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta
    15781 agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga
    15841 ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct
    15901 gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc
    15961 agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg
    16021 gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt
    16081 ggactgattt cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt
    16141 gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt
    16201 ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa
    16261 agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta
    16321 gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc
    16381 aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc
    16441 caatttccta acacttttgt atagtcattc gttgtaaact tcgggtagcg atgctgttcg
    16501 ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc tgttggccca
    16561 agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc ttgaccgctg
    16621 ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg aatttcagcg
    16681 gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct ttcatcaacc
    16741 accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt ttcaaaaatc
    16801 aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc ttgaccatcg
    16861 atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc tgttccacga
    16921 acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg tattctacct
    16981 tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc ttctttttca
    17041 atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc ctcctttgtt
    17101 tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg gaaatatggt
    17161 ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc tttcacatcc
    17221 ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc
    17281 taattataat ttaatttaat tttaatttta aatcaaatga tatccttttt agttaaaaaa
    17341 caccaattat acaacattta ttattaaaat taaattataa aattttataa cgttctcatt
    17401 taacaaaact taagttatta agacaaatga ataaacaact acgctttcct cgaaacaaaa
    17461 aaataaagca caaatgcgca tttacgcact catgctttat tcgtacaatt aattcagata
    17521 acggcagcgc ataggatgcc aataacactt cctaaaatag ctcctcctaa aatgtcagta
    17581 ggataatgta aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa taaaaaggct
    17641 aatattcctg caactggact taattcgaac aacgcagcta ctgataaaaa ggctagcgct
    17701 gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac aagccgacga
    17761 tttttttcat aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa accaacaaaa
    17821 aaatagatca aactgccgat aagaatggct agaccatatt ctctagtatc aggtatcaag
    17881 actaaaacag cagcaaggag caaccaaata aaaccaccat tagagatatt actgataaat
    17941 ttcaaaaaat tgatttttcg actatccgtt ttccgttgct tggcgaactt gttgataata
    18001 ctatcatcga ttttagcaat caaacgcaac atgttattcc tcctttttaa gaaccttttc
    18061 ttgcgtacga tggataaaaa gcaggacttg aaaataaaac aagaaactga aacctaataa
    18121 agcaaaatag attggtcctg caaaccagaa tgcgacaaga aggatcaacg tgcctgccaa
    18181 atagaaaagc gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa tccaagaagc
    18241 ttctttttgt tccagttgaa gcaagatttt gtaactaaag agaagcgcgc ttaatagaag
    18301 caaaaacaac gtagtataca tcaatgtata gccaattccc ccaccccaat agcggaaaat
    18361 ctgataatcg atcaatagaa gaagaaaata aacacttgct aaactatctt gctttccata
    18421 aagagagaag ttctcacgaa actttttcca gaatagtgaa ccgagctttt tctcttcatt
    18481 ttttttgtaa tgataaatca tatacagaac tgttcgactt gcagttccta atgtgaaaac
    18541 aggcaagacg cacaggaacc atagtagctg taaatggata agacgtggaa tccattcaat
    18601 gatttctaag aaataatcat tggctttttt accaatcgat cgattcatca tccttttgaa
    18661 cctcctgtca aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa cagcaaaatc
    18721 gtcgggatca agacaagtgt tgctcctgcc atcagataat tccattgtgt cgttgcttgg
    18781 ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt cttgatcttg gatatataac
    18841 agaggaccta agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat ggtaatcaag
    18901 gcaggtttcg tcaatggaag catcaaatga ctccaaatat acaaatgatt cgctccgtcg
    18961 atttcagcag cttcgatcaa ttcattattg atcgataagt aaaattgacg catcagaaat
    19021 atattgaacg cattgccgaa gaacgatgga acgatcaggg gcaaataagt acctacccag
    19081 ccgatcttgg agaataatac atattgaggg atcatcgtta caaaccccgg gatcatcatc
    19141 gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt cgcaaatcca
    19201 taagcaataa aagaatttga aaggacatta ccgatcacta ctagcacagt gatgaataat
    19261 gtatttcttg catagcgcca aaaaggaaag gcggcaatcg tatccagata gttttcccat
    19321 acgatttttt ctgggaaaaa ggtcggagga taacgaacga tttcttccat tggtttcaat
    19381 gaagtaaaca ccatccaaag caatggaatg atcaaaatga ttcctattac tgtcatgagg
    19441 acataactta gtattttttt tgtttgccga ttcacactgg tctttttgaa ataatccatc
    19501 atttgccgtc acctccagaa taatagaccc acttaggtgc aagcttcaaa ttgacaaacg
    19561 tcagaaccaa cacgatcaga aacaggatca ttgacatagc catcgcataa cccatatcaa
    19621 aagctacgaa cgccttgttc cacatatgca gattataaaa aagcaaagaa tttgcaggtc
    19681 cgcctgttcc atttttcgtc atgacataag cttcttgaaa aatttggaaa gaaccgatcg
    19741 tagaggtgat cacatcgtaa aaaataatag gagtgattaa tggcaaagta atacttttga
    19801 actttcccca aacgcctgct ccatcgattt ctgctgattc atataaagat ctaggaacat
    19861 tttgtaatgt agctaagtaa agtagcattg ctcctccaga actccatatt ttcatcaaaa
    19921 tcaacgcagg tttcgtccat tgtggatcaa acagccaagc tggtccaggg atatggaacc
    19981 agcccagaaa tgtgttgatc aatcctgcag atggagaaag cagctgcatc cataagatgt
    20041 agactgctac tcctgataag actgccggta aatagaaagc tgtacggaat acactgattc
    20101 ctttgatttt ttgattgagt actgtggcta aaaacacact gaaaatcgtc gtcagcggta
    20161 cattgaagat gacgaaataa acggtgttgt ataacgattt ccaaaataaa tcatcgtgaa
    20221 cgaacattct tttgaaattt gctagaccaa tgaaattcat cttggatgtc aaattataat
    20281 cggtgaaact accaataact gaaaaaagca taggccctag cataaatacc aagaacccga
    20341 taataaaagg cgagataaat aaataggccc atcttgcttc ttttttcttt cggatagata
    20401 aacgcttcaa attttcttgc ctcctctgta cctagacagt caaaaatgga ttattgattt
    20461 tgcttgacta agttttctac tgctttttgt gcatctgcca atccttcttt tggtgatttt
    20521 gttcctaaaa gaatctggtc aatctgttca ttcactaatg tcgtgtaatc cggagcatct
    20581 aaaggaactg gtgtgatcac cgtattttta aagttttcgt ccgccatttg atagatcatt
    20641 tttcctttat ccgttaggtt gtcattattc actaaattct cattggctgt cttgctcgcc
    20701 atgatatcaa aacttttttc gccgaatttt tcttgtactt ctggagaaga aagatatttg
    20761 atgaattcat aagaggcctc aggatctttt gctccgtatg gtacttccaa gacgaatccg
    20821 ccaccccacg accaatggcc tgaaccttct tctttttctg ggagctgcat cacgccaaaa
    20881 tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat aattgatatt ttgggcacgc
    20941 atggctacta atccggaaat aaatggatca gatacaccag acccaaattc ggcttctaat
    21001 ttattgatcg tatttttccc ataataatct tgccagtctt ggatccactc caaggcttcc
    21061 actttatttt tagtatcaat cttgactttt tcatctttgt caaaccaact agtcccgtta
    21121 tctgcgttta gcgcccatac atcagcccct aaattccata atggataaaa accgatgcgt
    21181 gtaaattctt ttccttcttt tttatctagt ttacgtgctg tttcttctaa ttcatcccaa
    21241 gttgtcggta gatcttgttc actgattcca gcttctttaa ataacttttt attataaaaa
    21301 atgacttgtg tatctgtatt gaatgggatc gcataaggat caccttcata ttctactgta
    21361 tcccaaagct gcggataaaa atccttagaa gtgtcttttt ctacatattt ggagagatta
    21421 gttgcttgct gtgcttctgc tctttgggca acggaattta tgtcctgtac aataacatcg
    21481 ggtgcatttc cagctgttat tgcagataaa gatttagtcc agatgtcacc ccatggctga
    21541 tacttataat ttacatggat ctcgttttgt gattgattga aatcctcaat gatttcttcg
    21601 atagcttctt ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt ttttcttcca
    21661 tcacttgttt tttcagaaga atttgctgca cttgaactgg catcgcttgc attacttgat
    21721 ccgcatcctc ctaataaaaa caatgctgaa cttgccagca accctgtaaa caataatttt
    21781 ttcatatttt acctccaaat tttaatgtaa ccgatgccgg ttttaatata acgatatcat
    21841 tttgttctga aaataacaat ttataaggta tgattctaaa aaaattatta atgataccaa
    21901 tactttatat aattcgcgag cgctttcttt gaatttttta atctttcgta gcagaatgag
    21961 aatgtattaa aaatagatta ggaggaattt tgatgtttag acaccagaaa gaattgcagt
    22021 ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa atatctgcag gagctgatcg
    22081 gcggccaata tggcgagatg agtgtagcta tgcagtacct gttccaaggc tggagctgcc
    22141 gaggagaaga aaaatataaa gatatgctga tggacattgc aacagaagaa atcggccacg
    22201 tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt gaagatcaag
    22261 aagcagcgat gcaaaaagat ccaacaatcg gtgccattat ctctggaatg aatccacagc
    22321 acgcgatcgt agatggctta ggacctcgtc ctgctgacag cgtgggcaat ccatggagcg
    22381 gtgcctatat cattgcgagt ggcaacttgt tagcagactt tagagctaat ttaaatgccg
    22441 aatcacaagg aagacttcaa gtagcacgta tttacgaaat gatcgatgat cgtggcgtaa
    22501 aagatatgct gtctgttctc cttgcaagag atagctatca tcaaaatcta tgggaatgtg
    22561 cagtgaaaga attagaagaa aaagaaggtt caatcttagt cccaagtact ttcccgcgtg
    22621 acaaagaacg aaaagacatt gcttatgact tctataactt ctcagaggga gaccaaagta
    22681 gtgaaggttc ttgggctagt ggcaaagcac tggatggcga aggcgaatat caatggttga
    22741 aagaacctaa gatcgaaggc gctgcaccaa aattagaacc agtaacacca aaaatgtatg
    22801 ggacacctcc attcaagaaa taaatatcta cccgtagaaa cacccgtttt ttgttccaca
    22861 cgctttgtga gcaaaactga ctaaagtcat taatcataac cacccttcaa aagggtggtt
    22921 ttctctagag ctataagctc aaatcaccgg ccagcgccta aagacgctgg ctttcatatt
    22981 ttatgttcaa gccatattgc ctttgccagt tttgcccacc tcttaaaagg tttatgtact
    23041 actccttagc ccttaaaagg atcgtcttac tctcgtacac tcagcttatc taacgcacta
    23101 tcatgttttt cttgttcttg aatgtacttt ttgatggtcg cttcattttg tcctactttt
    23161 gtcacatagt atccttccac ccaaaagtgt cgatacccaa atttatattt tagatttgca
    23221 caatctcttg atgatctcct gaatattggc tcgatattga ttataaatta tttttcttct
    23281 atactttggt gtgaatacaa tgtggtattt acacatctac tttgtacggg ctaaactatt
    23341 tccctttatt gctccacccg catagcaagt ggttttgtat ttcaatcgct cggcgcgctc
    23401 aattaggtct aaagacaata aatctaaaaa cccgctgctt cactgacaga aacaacgggt
    23461 tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa tctattttat ttttttgatt
    23521 ttttataaat ggaaaatgct tcaagaagtc caagaaataa agccgtataa aaacaagaac
    23581 ctatgaaatc tttatttatt agatattcaa cagaaatccc gataacacta gccaacaata
    23641 tatttatcag aagtgtaaaa taaggatgct ttttatagaa tagaactagt gttgttttca
    23701 tagactctcc tttcttcata tttccaatta atcacaaatt cacggcatta ctagaaaaat
    23761 taaaggggcg cagaagtgga aaaatactct tcgactttcc ctacaaacca ggccatcttg
    23821 tgtttaataa ctataccaaa tctaaggtac ctctaaatct aaattccatt tgtggcggtg
    23881 acctccaatt tttaactctt ctatctccca tccagtatac ataaccttga tacttcgacc
    23941 acttatatat cgaacacaca aatacctatc cattgcagga gtactaccta acttagttgc
    24001 tacttgcatc taaataacag ctgaaaagat aggacttact cttagtaaag tatctgaact
    24061 attaattttt aaactatcat ttttatcctc aactacatgc acatctgttg caacttcact
    24121 ttctgtctca tccagataaa tgattaagga atcgggaatt gcaatatctt cagctgacgc
    24181 tggaatagtt gagctaaatt agtcatcata tctttattat aaaataacca acagcaaaaa
    24241 atacaatgaa aaacaaactt ggaactaatg tatcattaat aaatgttgaa aaattcagca
    24301 actcattact gtatgatttt agcagcccaa ccaacatata ccctataaaa gctaataaga
    24361 tggttaggaa tcctttagaa tttttactca ttcatagaca cttccttaaa taacttttat
    24421 tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaacctt
    24481 acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc
    24541 gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact
    24601 aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact ttggttcact
    24661 atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa
    24721 taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga
    24781 tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca
    24841 gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc
    24901 atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa
    24961 aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg
    25021 ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa
    25081 tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat
    25141 gccatcgacc acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat
    25201 gtttagcata taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa
    25261 tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt
    25321 ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt
    25381 caggtaatga gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg
    25441 aaaacattgt tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt
    25501 gaatttcttt tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa
    25561 tataaattct ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca
    25621 ttcgtctttc tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg
    25681 aactaacgta attgttccat catcagaata cacaacgata tattcttggt tttctgaagg
    25741 tttctttccg ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt
    25801 tgtaacaagc attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc
    25861 aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat
    25921 ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga
    25981 aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt
    26041 caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat
    26101 gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa
    26161 gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca
    26221 aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga
    26281 gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga
    26341 taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac
    26401 tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa
    26461 taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac
    26521 tgtgacccta tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt
    26581 tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat
    26641 gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag
    26701 aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat
    26761 ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt
    26821 catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca
    26881 taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt gcaaaaatgg
    26941 ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca tcaattgtcc
    27001 ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt tttcttcctc
    27061 ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta attctgctgc
    27121 ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct ctgactctag
    27181 atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct atcatacact
    27241 gcaacaagaa gttcgcacca acaaaacgct ttcaattttt gtagaactaa aaaagttgtg
    27301 acaagtgaca aactctccag tgaatggtat tctcctgcca actcctcggt cacaagtcac
    27361 aatattcaac aaacatgaga agctgctttt tgaaattgtt ccttgtgact cggagtgaaa
    27421 cggcaggctt acgacctctg ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc
    27481 aaatttatgc aaaaatgtag aaagctattt tcacaaaatt tttcttaata ctcgaaacta
    27541 agcgactttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata
    27601 agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat
    27661 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagcta accttcggta
    27721 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
    27781 ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa
    27841 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    27901 ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac
    27961 aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc
    28021 aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct
    28081 tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat
    28141 tgattgacta attctagtag caacagacgg tattcttcat ccactgtact caactcaata
    28201 atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc
    28261 cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg
    28321 aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt
    28381 tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct
    28441 cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc
    28501 ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta tattatacag
    28561 taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac
    28621 agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag
    28681 gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt
    28741 gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac
    28801 caattgcatc tgtcgctacc acaacattga atccactctc taccaattca cgcatatggg
    28861 aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc
    28921 tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga
    28981 tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt
    29041 ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc
    29101 ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga
    29161 gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt
    29221 atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga
    29281 cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat
    29341 tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt
    29401 gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga
    29461 cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga
    29521 ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg
    29581 tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac
    29641 ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg
    29701 ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga
    29761 tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg
    29821 tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt
    29881 ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt
    29941 ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg
    30001 ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta
    30061 aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa
    30121 cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag
    30181 ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa
    30241 tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat
    30301 tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt
    30361 gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga
    30421 tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct
    30481 ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga atacagccga
    30541 tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac
    30601 cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat
    30661 tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa
    30721 tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca
    30781 gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca
    30841 atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc
    30901 gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat
    30961 actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact
    31021 gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa
    31081 gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga
    31141 ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca
    31201 tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat
    31261 ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc
    31321 ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact
    31381 cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt
    31441 tatatgattg attttaagat acatatatac cagaaagttg accccataaa ccaatactgt
    31501 taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca
    31561 ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg
    31621 tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta
    31681 ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct
    31741 catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc
    31801 gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct
    31861 tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc
    31921 ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac
    31981 cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat
    32041 ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat
    32101 ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat
    32161 aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg
    32221 aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga
    32281 tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat
    32341 ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt
    32401 ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca
    32461 aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc
    32521 caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta
    32581 gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt
    32641 ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa
    32701 aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat
    32761 aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt
    32821 gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt
    32881 gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat
    32941 gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc
    33001 agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc
    33061 cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt
    33121 tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg
    33181 tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac
    33241 aacttcaggg taagccactt catctagcca attcgttacc acatcggcac catttcgttc
    33301 agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc
    33361 tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac
    33421 tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt
    33481 tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc
    33541 aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt
    33601 agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat
    33661 tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca
    33721 aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt
    33781 gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg
    33841 ttgctggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca
    33901 tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg
    33961 cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa
    34021 aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt
    34081 ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca
    34141 tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat
    34201 tcgggtcgcc gatccctctg atgctaaaga atgatttttt aggaacctct agtatttgcg
    34261 ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc
    34321 ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat
    34381 cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat
    34441 aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc
    34501 tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag
    34561 tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca
    34621 tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat
    34681 gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga
    34741 atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta
    34801 taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag
    34861 gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc
    34921 tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa
    34981 ttggccccac ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt
    35041 ttcttgtgcc cgttttgttt ctttctttga ttatcggctg gtttgctatt cccgagatgg
    35101 cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta
    35161 gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca
    35221 tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga
    35281 tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt
    35341 atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag
    35401 gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc
    35461 tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg
    35521 ggctgattct ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag
    35581 tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca
    35641 atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa
    35701 tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta
    35761 tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa
    35821 aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt
    35881 atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt
    35941 ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat
    36001 cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt
    36061 tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc
    36121 ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt
    36181 tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac
    36241 acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa
    36301 agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc
    36361 acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg
    36421 aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca
    36481 ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac cgtttggaga
    36541 atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga
    36601 attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc
    36661 ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc
    36721 tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt
    36781 ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga
    36841 tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc
    36901 agatttttcc ccatttttcg gcgtactgcc agatgatcca agcttactgg ctgctagcgg
    36961 tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt
    37021 aaatactggt cattggccta cggaagatta tcaaaaggac atctccacct atattacgaa
    37081 aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga
    37141 ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc
    37201 ttttgcttca tctggcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat
    37261 atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc
    37321 tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg
    37381 ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa
    37441 atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc
    37501 ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag
    37561 ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt
    37621 tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc
    37681 atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg
    37741 tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag
    37801 gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga
    37861 aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc
    37921 agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga
    37981 gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa
    38041 tacagccatc gtataggtct ggtcattact agcatattgt ttcatcccac cgtggcaggc
    38101 aataaagcca ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt tttttaattc
    38161 tccaaagtaa cgtaatccta gcacagccgc tgtattatgg agagcatcaa acaaaatgag
    38221 tccatttgga aatgtttcat gtgtccattc gggatcacaa aagatataaa gatctccctc
    38281 atcaaaggaa acagactttt gataagtctc taagatcttc gaattcgctg gctgaaaatt
    38341 cagcaaataa gaatacaaat tgttctcaaa tccttctgga atcagcaaat gactttgcac
    38401 agaaaattct tcagataggc caacgattac ttttgtatgg taaaactggc gcttgcgact
    38461 ttgatacaca gcttctctta aaattttctc gtatttgctt tgttccttgt ctaagtcctc
    38521 aaagatccgc cgagcacttg ctgttcttcc cacaatgctt ccatcattga atactagtgt
    38581 cctaacatca gctggtaaat ctagttcttc agcatgtgag attgttaaat ctgtgatgat
    38641 cgttcctgga ctcccttttg ctagttgata agctgtcttc ctatcaaaaa cttcatgaac
    38701 attattaccg taaaatgtcg tttctacaat cgatcgaata ctggataaca gtggatttcc
    38761 acttgtgatt tcgtttaatt cgagactctc ttttgtactc atggctcctc ctcattccct
    38821 aaacaaaaaa acgttttctt tattatacat tattcgagtt tttatttgta ctgctgcgct
    38881 gtgggaagca aacgagttat ttttcacaca aaaaaaccga gaacttgaat aaacaagttt
    38941 ctcggtttca tcattttcct tttatcggct tagtaccagc cgtttgctaa ccagaatgtt
    39001 ttagcagcat cccatgaacc ataacgagat gttacatatt gttctgctac acgttcttga
    39061 ttggcagctg aatagtcgcc gtttaagtat gaagcatcta attgataacg gccgatgtaa
    39121 cgtccgttag tagctgtgta tgaaccgcct gattcttttt gtgcgatcca ttcttttgca
    39181 gagcttgaag ttggcgctgt tgcagcagct ggggcagcag gagctacttc aactgtttca
    39241 acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct
    39301 gttgttttgt tttctgttgt tgctgaagaa tcatttgaag ttgggattgt taattgttca
    39361 ccaacataaa tgcggttgat gtttgcgatc gaattttttt cagcaattgc atcgatcatt
    39421 gagttgtctc ctgcaaattt ttgtgagatc ttagataatg aatcacctga ttttactgta
    39481 taaacttcat cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc accagcagca
    39541 aaagttgtac ctaaaacaat tttcttaaat gaattcatat taaactcctt tttttctccg
    39601 ttttttattc tttcgacgct attaacttta tcatttagaa atatttcata
//
LOCUS       DAYYAJ010000009        38680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000009 DAYYAJ010000000
VERSION     DAYYAJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.009"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHATZ_000431"
     CDS             23..646
                     /locus_tag="ACHATZ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402989.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHATZ_000432"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHATZ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8402990.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHATZ_000433"
     CDS             2142..3023
                     /locus_tag="ACHATZ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8402991.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHATZ_000434"
     CDS             3026..3796
                     /locus_tag="ACHATZ_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8402992.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHATZ_000435"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHATZ_000435"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8402993.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHATZ_000436"
     CDS             5107..6270
                     /locus_tag="ACHATZ_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8402994.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHATZ_000437"
     CDS             6387..6797
                     /locus_tag="ACHATZ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8402995.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHATZ_000438"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHATZ_000438"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8402996.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHATZ_000439"
     CDS             9370..9654
                     /locus_tag="ACHATZ_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8402997.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACHATZ_000440"
     CDS             9814..10050
                     /locus_tag="ACHATZ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8402998.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACHATZ_000441"
     CDS             10047..10445
                     /locus_tag="ACHATZ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8402999.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="ACHATZ_000442"
     CDS             complement(11112..12056)
                     /locus_tag="ACHATZ_000442"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8403000.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="ACHATZ_000443"
     CDS             complement(12352..13296)
                     /locus_tag="ACHATZ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8403001.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="ACHATZ_000444"
     CDS             13517..14731
                     /locus_tag="ACHATZ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8403002.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="ACHATZ_000445"
     CDS             14753..16768
                     /locus_tag="ACHATZ_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8403003.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="ACHATZ_000446"
     CDS             complement(16851..18224)
                     /locus_tag="ACHATZ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8403004.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="ACHATZ_000447"
     CDS             complement(18250..18534)
                     /locus_tag="ACHATZ_000447"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8403005.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="ACHATZ_000448"
     CDS             complement(18536..20551)
                     /locus_tag="ACHATZ_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8403006.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="ACHATZ_000449"
     CDS             complement(20730..21035)
                     /locus_tag="ACHATZ_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8403007.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="ACHATZ_000450"
     CDS             complement(21124..22155)
                     /locus_tag="ACHATZ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8403008.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="ACHATZ_000451"
     CDS             22362..23237
                     /locus_tag="ACHATZ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8403009.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="ACHATZ_000452"
     CDS             complement(23389..24231)
                     /locus_tag="ACHATZ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8403010.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="ACHATZ_000453"
     CDS             complement(24323..26200)
                     /locus_tag="ACHATZ_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8403011.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="ACHATZ_000454"
     CDS             complement(26197..27618)
                     /locus_tag="ACHATZ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8403012.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="ACHATZ_000455"
     CDS             complement(27621..31010)
                     /locus_tag="ACHATZ_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8403013.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="ACHATZ_000456"
     CDS             31267..32658
                     /locus_tag="ACHATZ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403014.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="ACHATZ_000457"
     CDS             complement(32731..33378)
                     /locus_tag="ACHATZ_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8403015.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="ACHATZ_000458"
     CDS             complement(33386..34072)
                     /locus_tag="ACHATZ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403016.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="ACHATZ_000459"
     CDS             complement(34078..35151)
                     /locus_tag="ACHATZ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403017.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="ACHATZ_000460"
     CDS             35373..36047
                     /locus_tag="ACHATZ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8403018.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="ACHATZ_000461"
     CDS             36052..37392
                     /locus_tag="ACHATZ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8403019.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="ACHATZ_000462"
     CDS             complement(37541..38527)
                     /locus_tag="ACHATZ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8403020.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11910 a         7050 c         7321 g        12399 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttttaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga aggaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca
//
LOCUS       DAYYAJ010000010        36824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000010 DAYYAJ010000000
VERSION     DAYYAJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.010"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..890)
                     /locus_tag="ACHATZ_000463"
     CDS             complement(21..890)
                     /locus_tag="ACHATZ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8403021.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            1043..2503
                     /gene="rhaB"
                     /locus_tag="ACHATZ_000464"
     CDS             1043..2503
                     /gene="rhaB"
                     /locus_tag="ACHATZ_000464"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8403022.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            2500..3756
                     /gene="rhaA"
                     /locus_tag="ACHATZ_000465"
     CDS             2500..3756
                     /gene="rhaA"
                     /locus_tag="ACHATZ_000465"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8403023.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            3770..4615
                     /gene="rhaD"
                     /locus_tag="ACHATZ_000466"
     CDS             3770..4615
                     /gene="rhaD"
                     /locus_tag="ACHATZ_000466"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8403024.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            4617..4928
                     /gene="rhaM"
                     /locus_tag="ACHATZ_000467"
     CDS             4617..4928
                     /gene="rhaM"
                     /locus_tag="ACHATZ_000467"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8403025.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            5010..6161
                     /gene="fucO"
                     /locus_tag="ACHATZ_000468"
     CDS             5010..6161
                     /gene="fucO"
                     /locus_tag="ACHATZ_000468"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8403026.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            6288..7046
                     /locus_tag="ACHATZ_000469"
     CDS             6288..7046
                     /locus_tag="ACHATZ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8403027.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            7146..7790
                     /locus_tag="ACHATZ_000470"
     CDS             7146..7790
                     /locus_tag="ACHATZ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8403028.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            7797..8804
                     /locus_tag="ACHATZ_000471"
     CDS             7797..8804
                     /locus_tag="ACHATZ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8403029.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            8820..9662
                     /gene="kduI"
                     /locus_tag="ACHATZ_000472"
     CDS             8820..9662
                     /gene="kduI"
                     /locus_tag="ACHATZ_000472"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8403030.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            9682..10485
                     /locus_tag="ACHATZ_000473"
     CDS             9682..10485
                     /locus_tag="ACHATZ_000473"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8403031.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(10734..11798)
                     /gene="fni"
                     /locus_tag="ACHATZ_000474"
     CDS             complement(10734..11798)
                     /gene="fni"
                     /locus_tag="ACHATZ_000474"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8403032.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(11795..12880)
                     /locus_tag="ACHATZ_000475"
     CDS             complement(11795..12880)
                     /locus_tag="ACHATZ_000475"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8403033.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(12893..13870)
                     /gene="mvaD"
                     /locus_tag="ACHATZ_000476"
     CDS             complement(12893..13870)
                     /gene="mvaD"
                     /locus_tag="ACHATZ_000476"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8403034.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(13863..14807)
                     /gene="mvk"
                     /locus_tag="ACHATZ_000477"
     CDS             complement(13863..14807)
                     /gene="mvk"
                     /locus_tag="ACHATZ_000477"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8403035.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            15123..15782
                     /locus_tag="ACHATZ_000478"
     CDS             15123..15782
                     /locus_tag="ACHATZ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8403036.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            15867..16772
                     /locus_tag="ACHATZ_000479"
     CDS             15867..16772
                     /locus_tag="ACHATZ_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8403037.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(16774..17406)
                     /locus_tag="ACHATZ_000480"
     CDS             complement(16774..17406)
                     /locus_tag="ACHATZ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8403038.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    17557..17682
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17726..18250
                     /gene="infC"
                     /locus_tag="ACHATZ_000481"
     CDS             17726..18250
                     /gene="infC"
                     /locus_tag="ACHATZ_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8403039.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            18322..18522
                     /gene="rpmI"
                     /locus_tag="ACHATZ_000482"
     CDS             18322..18522
                     /gene="rpmI"
                     /locus_tag="ACHATZ_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8403040.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            18575..18934
                     /gene="rplT"
                     /locus_tag="ACHATZ_000483"
     CDS             18575..18934
                     /gene="rplT"
                     /locus_tag="ACHATZ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8403041.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            19186..20523
                     /locus_tag="ACHATZ_000484"
     CDS             19186..20523
                     /locus_tag="ACHATZ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8403042.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            20571..22700
                     /locus_tag="ACHATZ_000485"
     CDS             20571..22700
                     /locus_tag="ACHATZ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8403043.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            22675..23364
                     /locus_tag="ACHATZ_000486"
     CDS             22675..23364
                     /locus_tag="ACHATZ_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403044.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(23772..24449)
                     /locus_tag="ACHATZ_000487"
     CDS             complement(23772..24449)
                     /locus_tag="ACHATZ_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8403045.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(24585..24779)
                     /locus_tag="ACHATZ_000488"
     CDS             complement(24585..24779)
                     /locus_tag="ACHATZ_000488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403046.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(24829..25269)
                     /locus_tag="ACHATZ_000489"
     CDS             complement(24829..25269)
                     /locus_tag="ACHATZ_000489"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8403047.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(25273..26118)
                     /locus_tag="ACHATZ_000490"
     CDS             complement(25273..26118)
                     /locus_tag="ACHATZ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8403048.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            26267..27685
                     /gene="pepV"
                     /locus_tag="ACHATZ_000491"
     CDS             26267..27685
                     /gene="pepV"
                     /locus_tag="ACHATZ_000491"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8403049.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(27742..28689)
                     /locus_tag="ACHATZ_000492"
     CDS             complement(27742..28689)
                     /locus_tag="ACHATZ_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8403050.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(28912..29262)
                     /locus_tag="ACHATZ_000493"
     CDS             complement(28912..29262)
                     /locus_tag="ACHATZ_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8403051.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            29462..30541
                     /gene="pepA"
                     /locus_tag="ACHATZ_000494"
     CDS             29462..30541
                     /gene="pepA"
                     /locus_tag="ACHATZ_000494"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8403052.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            30685..31005
                     /locus_tag="ACHATZ_000495"
     CDS             30685..31005
                     /locus_tag="ACHATZ_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8403053.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            31027..31491
                     /locus_tag="ACHATZ_000496"
     CDS             31027..31491
                     /locus_tag="ACHATZ_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8403054.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            31696..32301
                     /gene="ytpR"
                     /locus_tag="ACHATZ_000497"
     CDS             31696..32301
                     /gene="ytpR"
                     /locus_tag="ACHATZ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8403055.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(32340..33629)
                     /locus_tag="ACHATZ_000498"
     CDS             complement(32340..33629)
                     /locus_tag="ACHATZ_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8403056.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            34057..34536
                     /gene="rlmH"
                     /locus_tag="ACHATZ_000499"
     CDS             34057..34536
                     /gene="rlmH"
                     /locus_tag="ACHATZ_000499"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8403057.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            34739..34864
                     /locus_tag="ACHATZ_000500"
     CDS             34739..34864
                     /locus_tag="ACHATZ_000500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403058.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            34861..35289
                     /locus_tag="ACHATZ_000501"
     CDS             34861..35289
                     /locus_tag="ACHATZ_000501"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8403059.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            35360..36529
                     /locus_tag="ACHATZ_000502"
     CDS             35360..36529
                     /locus_tag="ACHATZ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8403060.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT        11744 a         6695 c         7713 g        10672 t
ORIGIN      
        1 tgacgtacat attatatgaa tcagctgaat ttacgaaagt ctctcggcga tagtcctgtt
       61 tttgctttga acaggcggct aaagtaagca aaattctcaa tccctatttc ttcggaaatc
      121 attcgaaccg taaattctgt ataccgaaga aggtctttag cgtaagaaag ttttttgctg
      181 atcaaataat cgattggtgg agaaccgata aatttggaaa aatctttgct cagctgatat
      241 ttattcaata agaattgttg ttctaattga tcaagtgtaa tcgtttcttt gaaatgttcg
      301 tctaagtatt gcttcagttt gtaaacgtag gcgggaatat cctcttcttc gaaatctagc
      361 tgatactttt gcaaaatcag ttcattcagc aactcgtgga tagctacaga ggactggaaa
      421 tcggtacggg cattcgtgcc ttgttgcatg tggatcaatt ctgtgatcaa atggtggatc
      481 ttgttttctg atggatctcc tgttgaatgg aaggtgttat ggccgtcttt tgtgaactcc
      541 tgataaaatg attggacgtt ctgaccattc aaatggatcc aatccatttc ccatggttct
      601 tcgctgatcg tttgataacg ctgatattct tggcagtcaa taaaaaataa gtctccagct
      661 tttagctgtt gctgattatc attatatgta agcgaacctt tgccagacaa agtaaacttt
      721 atcaagtaag aaggtaaatg tgctcgttca gtatagtaag ggattggtgc tttgaagtaa
      781 ccgatttctt ggatataaaa aaataaattc ctgcttcttt gtgtaggcgt caagatcact
      841 ctttttgagt cttccgacca tgagtggttg gctttttccc agtaatccat gtaaacactc
      901 ccaacttgtc aagataatgc aattatatac caaaatagtg tgaagcaaaa tataagattc
      961 tgtattatga tagcattgtc aataaatgat aacgctttat taataaagtg ctaatcaaca
     1021 ataaagtaaa agaggtggag aaatgaatcc aacatttgtt gccatcgata tcggtgcatc
     1081 aagcggacga ctaatgaaaa gtacgaggga tgcaaaaggt tctttgatct tggaagaagt
     1141 acaccggttc aaaaacggct ttcggaacaa agacggtcac gatcgctgga agatcgatta
     1201 tcttatcaaa gagattttga ttggatttga aaaattaaag cagacaggga ttgatcgcct
     1261 ttttgcgggg atcgatacat gggcagtcga ttactgtctg attgatgaac aaggggatcg
     1321 gataaatgat ccgattgctt atcgggacaa gcgtaccaat ggagcagtag aacagttttc
     1381 tgaaatctat tctctagata agttgtatga gcagacaggg attcagatcc agcctttcaa
     1441 taccttgttc caattgtttg ctgaagaaaa agaaaatttg gcaaaagcac ataaattgtt
     1501 actgatacct gattatttag ggtatgtctt tactggtaac atggtgacgg agaaaactaa
     1561 tgcatctact atgcagctgc tgaatgcagg gacaaaagaa tgggaaatag aacttcttga
     1621 aaaaatcggt gtctcgaagg aattatttcc accattgaca gagccgggaa cgatactagg
     1681 agatctgcaa aaagaacaat tttcaaaata tgatttgcca gatgtgacct ttatcacagt
     1741 agcaagtcat gatacggcgt cagctgtatt aggaacgcca ggtcaaggag aacgttgggg
     1801 ttatatcagc agtgggacat ggtcgctttt agggattgaa acaaagatcg ccaatgtttc
     1861 tcgcaatgct ttttctgaaa attatacgaa tgaatggggt gcccataaca cggtgcgttt
     1921 tttgaaaaac atcatgggga tgtggttgat ccaggaagtt gcccgaatgc aagagtacga
     1981 gtacagttat gcggaattgg cagagcttgc tgcaaaagaa aaaccgctgc agcaagtgat
     2041 ggaaatcaat gaccctcgat ttttgaatcc cccaaatatg atagaagaga ttcagcaata
     2101 ctgttttgaa acagagcagc cagtccctgt ctcatcggga gctatcgctc gttgtgtgta
     2161 tgataatctt gctctttgtt acgcacaaga gctgatcaaa gtagaacact taactggaac
     2221 gaaattggaa aagctgcaca tcgtaggggg cggatcgaat aatcgtctac tgaatcaatt
     2281 gacagcagat tatgccggaa tcattgtaga agcaggacct agtgaagcca cagcaatcgg
     2341 gaatgttgcc atgcaaatgc tcagtacagg tcagttttcg aatatagaac aagcaagaaa
     2401 tgtaatcaaa cagtcgtttc catgtgaaac tttttaccca gatgaaaata agcaaaacac
     2461 gatggaatgg tacgtgaata aaatcaaagg agtaatcgta tgaacacgat tgaaagaaat
     2521 tacgaacaag caaaagaaaa atatgcaact atcggtgtag atacagatgc tgtactagaa
     2581 aagatacagg acatcaaaat ttctatgcat tgctggcaag gagatgatgt caaaggtttc
     2641 ttgacaccgg atggcgaatt gaccggtgga atcatggcga cagggaattt tccaggagcc
     2701 gcacgaacac ctgaagagct tagacaagat ctggagaagg cttattcgtt gataccgggc
     2761 aaacacaaat tgaaccttca tgctatctat ttggatacag aagaatctgt cgatctaaat
     2821 gaaattgagc cgaaacattt tgaaaaatgg gtagagtggg caaaaaaaga agaaatcggt
     2881 cttgacttca atccgacttt cttttcgcat ccaatgatga aagacggctt tacattggct
     2941 catccagata aagaagttcg tgaatactgg atcgaacacg ggaaaagaag ccgcaaaatc
     3001 gcggagtact ttgggaaaga aactgggcaa acaagtatca ataacttttg ggtacctgat
     3061 gggatgaaag ataatccgat tgatcgtttt accccacggg aacgtttaat ggaatcactc
     3121 gatgaaattt ttacagaaga gttagatgaa cggtatgtac aagatgcagt agaaagcaaa
     3181 ctatttggtt taggcgctga agcttataca gtcggttccc atgaatttta tatgggttac
     3241 gggttgactc gtgacaaact tgtttgtcta gatgctggac attttcatcc aacagaagtg
     3301 atttccaata aattatcttc attagcttta ttcagcaaag gaatcatgtt gcatgtttcc
     3361 cgtccagtgc gttgggattc agaccatgtg gttttgatgg atgatgaatt gcaggaaatc
     3421 gctaaagaat tagtccgtaa cgaattgttg gaaaaaacaa atatcggtct ggatttcttt
     3481 gatgcaacga tcaaccgtat cgctgcttgg gtcatcggta cgagaaatac acaaaaagca
     3541 ctgctgaaag ctatgctgga accagttgag cgattgaaag aaatggagct ggcttttgac
     3601 tttacttcaa gaatggctta tacggaagaa ttgaaagatt tcccgtatgc cgatgtatgg
     3661 aattatttct gttacaaaaa tcaagttcca gttggtttgg attggctgga agaagtccag
     3721 gaatatcaaa aagacgtgtt ggaattaaga aaataggagt gtggctatat tgaaaaaaat
     3781 agatatttta caagcgcctt atgtgaaaga aatgatcgaa acgacgtcga atctttatcg
     3841 cttgggatgg gatgaaagaa atggcgggaa tatttcttat ttactaaaag aagaagagat
     3901 cacaccgttc ttggatcctg aaaaagtgat ccgccgcatc tcgatgattt ttgatgccag
     3961 ccgtttagca ggatgctatt ttattgtaac tggttcagga agatatttta aaaatgtagc
     4021 gagcgaacct gccgagaatc ttggaatcgt tcgagttgct gaagatggca aaacactgga
     4081 acttttgtgg gggctggaaa acgaatgcct tcctacaagc gagttgccaa gtcatttcat
     4141 gagtcatatc gcccgtttag aagtagaccc agataatcgg atcgttatgc attgccatgc
     4201 tagtcatcta ttagcgatga gttttacgca tgaattagac gaacgttcct ttagtcgtac
     4261 gctatggcaa atgtgtacgg aatgcttagt tgtttttcca gaaggaattt ccatcattcc
     4321 gtggatggtt ccgggaacaa atgagattgg ggaagcaaca gctgaaaaaa tgaaagaaac
     4381 tcgattggtt ttatggccac aacatggaat ctacgggtca ggaaaagaca tggatgaagt
     4441 ctttggtttg atcgagacag cagaaaaggc agcagaagtc tatacgtacg tcaaagcgca
     4501 aggaccaatc ttgcaaacaa tcacggacga aaacctgtgg catttagcag atgcgtttgg
     4561 tgtcactcca aaagcaggtt atttggagga agttcatacg aaagcaggtg tctgacatgg
     4621 tgaaaaaagc tttttgcatg aaagtttacc cagaaaaaca agaagaatac aaaaaaagac
     4681 atgatgaact ttggccagaa atgcgtacag cacttcgtga acacggggtc atcgactatt
     4741 cgatctatct tgatccgagt acaagtttgt tatttggtta cttagtgatc gaagatgaaa
     4801 agttatggga aaaaatggca aatacatcta tcaatcaaaa atggtggaag tacatggaag
     4861 atatcatgga gacaaatcca gatgcctctc cagtcagttg ggaattgatg ccagtctttg
     4921 aactgtagca acaagcaact gggcaaccgg ttgcttgggg ttctttcgtt tttattgtga
     4981 aaaataatac aaattaggag gaatagaaaa tgagtaatcg aatgatttta aatgaaacat
     5041 cgtattttgg aaaaggagcg attgagtcga tcgtccctga atttcagcag cgaggattcg
     5101 gcaaagcagc tgtcattact gacaaaggat tgatcgagca cggtatagca acaaaagtaa
     5161 cagaacttct agataaagca gcaatcgctt atgctttata cgacgggatc gtacctaatc
     5221 caacgatcca aaatgtaaaa gatggagtgg cttttgttaa agaggcagag gcagactgtt
     5281 tgatcgcaat cggtggaggt tcgccaatcg atacggcaaa agccattggg attattttga
     5341 cgaatccaga gttttcagac gtcattagtt tagaaggtgt tgctgatacg aaaaatccat
     5401 gtttacctat attagctata ccaacgactt ctggaacagc agcagaagta acgatcaact
     5461 acgtcatcac agatgaagta aatcaccgaa aatttgtttg tgttgatcct catgatatcc
     5521 caatcgtcgc gttcatcgac agcgatatga tgatgggaat gccgaaaaaa ttggctgcat
     5581 caacaggtat ggatgctatg acgcatgcta ttgaagggtt tatcacaaaa ggcgcttggg
     5641 aaatgactga tatgctgcat ttaaaagcaa tcgaaatcat cggccattct ttggaagctt
     5701 ctgtagatgg cgatcaaaat gggcgagaaa aaatggcatt aggacagtat attgcgggaa
     5761 tgggcttctc aaatgtcggt ttaggactgg tacacgggat ggctcacccg ttaagtgcat
     5821 ggtacaacat tcctcatggc gtagcgtgtg cagcactttt gccaactgtc atgaaatata
     5881 acaaagaata tactggagaa aagtaccgtg agatcgctct tgttctaggg attaaaggag
     5941 cagctgaaat gtctttagaa gatgtaagag aagcggcttg tggtgagatc gatcggttga
     6001 gcaaagcggt tggaatccca gaaaccattt ctgaattagg tgtaaaagaa gcagatattc
     6061 cagcaattgc agaagatgct ttgcgagatg tatgcacacc aggaaatcca agagaaacga
     6121 cagtagaaga aatcattgct ttatatcaaa gtttgatgta aaaacgaagc ggatcttcaa
     6181 cattaaattg cctgtaaaga gaaatggtag tttatatctg gatatctctt ttaaattgat
     6241 ggtatttccg ctataatttt tcttataaag aaataggaga atattatatg gaaaataaaa
     6301 agccttatgg aactgtattg atcaaagcct ctaaaatact agactattta gcggaaaacc
     6361 aagatgtctc tttgcagacg atcgcaaagg gtgtgggaat gacctcttct acagtcttga
     6421 aaatactaga tactttgttg atgatcggct acgtaaataa aaattcagag aaaaactatc
     6481 gtttaggtgc aaagcttatt cgctacgcga ataaaaacat tgaacaaatc gatttggtag
     6541 agttgacttt accgttttta gaacagctgc agcaaaagat cgatgagact atccacttag
     6601 gggtattgga caacaatgag atcttgtacg tgaacaaact agatccaaaa catcaaacga
     6661 tccgcatgtc ttcaaaaatc ggaatcaccc gaccattata caattctgcg atgggcaaag
     6721 ccgtattagc agaattcagt gaagagcagt acactgatta tcttgaaaag catccgttga
     6781 tcccttacac agaatatacg atcaccaatg cattacggtt gaaaaaggaa atcaaaacgg
     6841 taaaagaaac ccacatcgca tttgacgatg aagaaatcga acgtgatatt ttttgtatag
     6901 gagcctcaat cgtgaaaaac gaacaaatca ttggtgcatt tagtgtaagt atgccgaaat
     6961 atcgtttaac agatgagaca aaagaacaaa taatcagtgc cctcaagcaa acaaaaagcg
     7021 aaatcgagca aagaattcaa aaataatgaa aaaagcgttt gcaatttttc ttttctgccg
     7081 tataatatgg gtatagattt ccaagttata aaataaaatt tctaaatagg aaataggtgg
     7141 agaagatgaa aaaaattgat attttatctc gattgaaaaa cgccggagtg attgcggtgg
     7201 tacgcggaaa aagcaaagaa gaagctttga atgcttgcca cgcaatcatc aaaggtggtc
     7261 taactggtat tgagttgact tttacagtcc ctcaagcaga tcaagtaatc aaagaacttt
     7321 tgtcattcta caaagaccaa ccagaaatag tgattggcgc aggcacagta ttagacgctg
     7381 tcacagctcg cttggctatc ttagctggcg cagagtatat tgttagccct tcctttgatc
     7441 aagaaacggc agagatgtgc aatctgtatc aaattcctta cttaccagga tgtatgacta
     7501 ttaccgagat caaaacagcg ctgaaaagcg gggtagatat cgtcaaacta tttcctggaa
     7561 gcgcttatgg accaagtatc atttctgcct tcaaggcgcc gatgcctcaa gtcaatatca
     7621 tgccaactgg cggtgtgagt ctggacaata tgaaagagtg gttcgatgct ggtgtcgtaa
     7681 cagtcggagt aggtgggaac cttttagcac ccgcagctac aggagatttt gacaaagtaa
     7741 cggaagtcgc gcaacagtat gcagcaaaaa tgaaagaaat caagaggtag ctgcttatgg
     7801 gaaaagtagt gacactcgga gaaatcatgt tgcgattgtc cacagagcag ggaacccgta
     7861 tcgattcaag tgaacgattc gctgcccatt atggcggagg ggaagcaaac gtagcaattt
     7921 ctttagctaa ttacggacat gaagcgattt ttgctacaaa agtacctgat aacgcattgg
     7981 gaaaagcagt caaaaagcac ttgcaatgtt atggagtaga tacgaaacag gtattatctg
     8041 gtggatcaag actaggtact tattatatgg aaactggtag tggcgaacgc gccgcctcag
     8101 tgatctatga tcgtgccgga tcaagttttg ctgaaatgga tcatttggaa tggtcgctag
     8161 aggagttatt ccaaggaata gatatatttc atgtttcagg aatcacccca gctttgtcta
     8221 aaaaatggca aaaaatgacg aaacaggtga tagaagctgc aaaaaaagct ggctgcctga
     8281 tcagcttcga tatcaactat cgtggaaaat tatggacaca gcaagaagcc agcgaagtaa
     8341 tccatcagct tttaccttta gtagatatct gctcagctgg aaaaatggat gctctttatt
     8401 tgttaggtat cgagaaagcg ccagaagcag aagaacaacc tctaatttat tattatcaaa
     8461 aaatgcagga aaagttcccg aatattcagg tgttttattc gaccaagcgt accgttcatt
     8521 cagcaagtga aaatgaattg aaggggacat tgtggatgaa ccatgaatat gtggaatccc
     8581 aagtccatag aatcacccca atcgtggatc gagtaggtgg cggagatgct tttgcaggtg
     8641 gcgtgctcca tgggatttta tccaatatgt ctccgcaaga aatcattgat tttgctacgg
     8701 cagcttctgc cttgaagcat acgatccatg gggactgcaa ccaatttagt caagaagaag
     8761 tcgcacaatt tttagcatgc ggctctggaa aaatcattcg atagaaggag tagaagaaaa
     8821 tgcaagaaat ggaaacacgt tatacacaca gtccagaaga tatccgtcat tattcaacgg
     8881 aacaattgag aaaagaattt ttagtagaaa aggtatttga accagggaaa atcagtttga
     8941 cttatacgca caacgatcga atgatctttg gtggagtgac acctactgaa aaagaattag
     9001 aaatcacatt agacaaagaa ctaggtgttg attacttctt agagcgtcgt gaactaggtg
     9061 tgatcaatat cggtggacca ggattcatcg agatcgatgg gcaaaaagaa gaaatgaaaa
     9121 aacaagacgg atactatatc ggaaaagaaa cacgacatgt tgtattttct tctcaagatg
     9181 ctgaaaaccc agctaaattc tatattagtt ctgttccagc tcatcataaa tatccaaatg
     9241 taaaaatcag tatcgaccaa gtaaaaccaa tggagactgg tgaaggcctg acactgaatg
     9301 aacgtaaaat ctaccaatac atccatccaa atgtatgcga aagctgtcaa ttacaaatgg
     9361 gttatacgat tttagaacca ggaagcgctt ggaatacgat gccttgtcat acacatgaac
     9421 gtcgtatgga agcttatgtc tactttgatt atgccaatga agatacacgt gtgttccaca
     9481 tgatgggcaa accagatgaa acaaaacatt tagttgtaga caatgaacaa gccattattt
     9541 cgccaagctg gtcgatccat tcaggtgtag gtacaagcaa ttactcattc atctgggcaa
     9601 tgtgcggaga aaatatcacc tatacagaca tggatatggt gcaaatggat caattaaaat
     9661 aagcataagg gagagaaaat gatggaattt agtatggata tgtttcgttt agatggaaaa
     9721 gtagcattag tcaccggtgc tgtctatggt attggatttg agattgcacg ctctttagct
     9781 acagcaggag caaaaatcgt atttaataac ttgaatcaag aatcagttga tgaaggtata
     9841 gcgcattata aagaagcagg catcgatgca aaaggttatg tctgcgatgt gaccgatgaa
     9901 gaagcggttc aagcgatggt tcaacagatc aaagaagatg taggttcagt tgatattcta
     9961 gtcaacaatg cagggatcat caaacggaca ccgatgatcg aaatggatgc ggcagacttc
    10021 cgccaagtaa tcgacgtgga tttaaatgcc ccattcatcg tttcaaaagc agttattccg
    10081 gatatgatcg aaaaaggcgg cggtaaaatc atcaatatct gctccatgat gagtgaatta
    10141 ggacgcgaaa cggtcagtgc ttatgctgcg gcaaaaggcg gattaaaaat gctgacgaaa
    10201 aacatcgctt ctgaatacgg tcaatacaat atccaatgta atggaattgg accaggttat
    10261 attgctacac cgcaaactgc tccattacgc gaaacacaag aaaacggtga acgtcatcca
    10321 ttcgatcaat ttatcgttgg acgtacgcca gcagcacgct ggggcgagcc aatcgatttg
    10381 gctggaccat ctgtcttctt ggcttctagc gcatctgatt tcgttaacgg ccatgtcttg
    10441 tatgtggacg gaggcatact tgcctatatc ggaaaacaac cttaaaagag ctttagtaca
    10501 ctgtttagtg gagaggttgt gaaatcagcg gattgaacga ataccgaagg acagctgatt
    10561 aaacatcatt tattcgaaaa aagaggctgg gacagaagca tttaactcca agaaataaga
    10621 aggaattcac gaaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggagt
    10681 tgcttttact tccgccgttt gtacgtgttt agagcgtgag acaaaagtgt tctttacttt
    10741 tgtcccacgc tcttttctgt gcgtcttcca tacttcacta agtcgatccc tcttgcttcg
    10801 caccaatcct tcactggtcc ggaaaagatc agagattgtt ggtgtagggc cgcggtattc
    10861 gttgcgccga ccatagtata aagcagacga agctcttctt gccattgctt catcagtata
    10921 atagtttctt ctacgccgtg attcatcaga tgcgtcagta cggttcctga agtacccacg
    10981 gcatttgcgc cgagacaaag ggctttaaat atgtcatagg cattgcggat acctccggag
    11041 gctaggattt ccatagaagt gtctgcttca tttgcttcaa gcaatgaaga gactgtcgat
    11101 tgcccccagt cagctaaata actcagttcc cgcttggagc gacgggcgtt ttcgatctgg
    11161 gtgaagcttg ttccgcttcg accgctgata tcaactgtat gaactcctaa agcagctaga
    11221 tcattgagcg tttctcttgt catgccaaaa ccgacttctt ttacgatgag ggggacgtca
    11281 atggctgttt gagtttcttg aataagtact ttccaattgg taaaatcacg gtctccctca
    11341 ggcataacca actcttgagg tgcattcaaa tgaatctgta aggcatctgc gtgaaacaat
    11401 cggatagctt ctttggctct ttcgacagaa gtccctgcac cgatgttggc aatgattttc
    11461 ccgtgaggat attcttgtcg cataatcgta taagtatcgg ctaaagaagg gactttcaag
    11521 gcagcgctga ctgaaccagt ggcaatcatc aaatctgctt ctcttgcgac gattgcaaga
    11581 tcttggttga ttttttttgt tttttcgctt ccgccagtca tcgcattgat ataaaatggg
    11641 ctgcttagtg taaacccaac tgcctgtgta cttagatcga catcagaaac tgctatttgc
    11701 ggcaaagggt tatggatgat ccgtacgaag tcaaattcgt ttttttgttt atcatgaaag
    11761 gctttagcta gtgaaacatg ttcgtctttt cgattcatcg gttttccttt ctttgatcgc
    11821 tatagacatg taacggcagt ggagtgattt ctgctttttc ccatgcactc attaaaggaa
    11881 gaatgccaga tttctggtca acaattacga ttccgcaatc gcccccacct gcaccagaag
    11941 attttgcggc tccttcatac tgttcagcta aattacacaa tttgttcaaa gcaggcgttt
    12001 cgatgacgac cccagtgatt gcagataaat catgcagtaa ttgtcggttt tttcgaatca
    12061 tctgttggat caacgttaca ttattttctt taaacccttt gatcatttcg ttgacacatt
    12121 ctgtactatt ttttaagaaa agctgataag ccaccatttt atcttctctc gaacggtgaa
    12181 cttgatcgac caaatcagaa gtagaggcag ggctacccgt ccaaccaatc aataaacgta
    12241 aatcttcagg agcaatcaat ggctcaatgg atagacctgg ccaatccagg gcaagtaact
    12301 cactgataga atgctgagtt tcttgttctt ggagccaagg atggtcgaag gttgagaaag
    12361 cgatccagcc accataacag ctagcagcga tgtcgccgca ggaaccatta tcttgaactg
    12421 ctaaattggc tagtgctgca atcttgaaaa tctccaactg agataaattc aacgcgtaaa
    12481 aaacattcaa ggctttgacc gttgcaacgg tcacggcacc gctagaaccc aatccatatt
    12541 tccggccatt tgaactgtct aactcacttg ttactttcag atcataaaag gataaaagaa
    12601 tgtttttttc ttgtgcatac ttttcagtca agcgaatagc agcaaggata taatgaaaag
    12661 gattttcccg aatatctaaa actaattctc cgttgcgtct tgtccaacgt acaggcatcc
    12721 cactatattg tgcagattgg atacttccga cttttcgtgc agattctaca gtgactgtca
    12781 cgaattgatc gactgcagcg ataactgctg gatggcctgt ttcaacaact gcatattctc
    12841 cggcaatata gagtttgcct ggtgcagata cttcaatcat aaaaatctcc ctttattcaa
    12901 taatcgcaat tcctggacca gcatgtgcag taattacttg ttctttgcta aataatttgg
    12961 taaatgtttc ttgtacctca gataaatggc tgttttccac taaaactttg acattagggc
    13021 cagcatccat cgtaaagtag catggaatgc cttgctttct caattgtcgc accgcatcca
    13081 tcgcttttaa gctgtcagga gaccagtaag tgaatggagg ctgggcagct aaagtcgttc
    13141 catgcatttt tagtccgttt ctttccatcg tttctccgag aagttggaaa tcttttgttt
    13201 tgattgcttg tttcaattga tatagatccc cttcaacgga atctaaccag ccttgataaa
    13261 aactggatgt ttcgactgtc cgacgcatcc catttctgct ggacacttct tttttctggt
    13321 cgttgatcaa aacgaaaacc attgctaaat cgtcttcgaa agaatcggaa gggactggct
    13381 tagcgtaaga acttaagtcg tcatggcctt tttcccattc gacgaaacca ccgaaaatac
    13441 ttcggcaagc tgaaccagac ccgcgtcgag caaatcttga aagagagaga tcatctaatc
    13501 caagcttaag agcagtattg caagcgcctg ctaaagcagc tagcccgctg gcagaggagg
    13561 ctaatccagc tgcggtagga acgaaattct gactgattac tgaagctttt agtgagaggc
    13621 cggcttcttt acgaaaaagg tcaagaaact gactgacttt ttttgttgct ttttcgtctt
    13681 gcaaggtgcc atctagataa aattcatcta ccatatagct atcagaaaag atgacttctg
    13741 tctctgtata aaatgcatcc aatgttaacg aaaggctgtt gttcattgga aggatgagtt
    13801 cttcattttt cttaccccaa tatttgatta gagcaatgtt ggtatatgcg cgtgctttgc
    13861 ctttaaacat aggtatgtac tcctaatcct tgtatccaag tttcagcagc acccgcatct
    13921 tcaagtgctt gactgatttc ttgggctttt gtttttgttt gtgctaatgc aatcatacag
    13981 ccaccgcgtc caccgccagt taatttggca cccaatgccc cggtatcttt tgctgtttgg
    14041 atcaatagat taagaaaatc attgctgatt gtcagctttt ccagaagtga ttgtgattca
    14101 tccattgtct gtgccaacgt ttctggtgaa ttttcaatga ttgcttgttt tgcttgcttc
    14161 gtcaagtatc ctaatttttg gatcatttgt ccagtttcat gaggctgggt ttcaaagagg
    14221 tgagcgacat ctttgacggc ttctcttgtt tgtcctttga ttcctgtatc agcgacaatc
    14281 aaaaaagcat cgatgttcaa agaaaagtag tcgaaaggat gcccttttgt aaaataaatc
    14341 ggctgcaagc tgctagtagc ggctgcatcg attccactag gattaccatg ggcgattttt
    14401 tccgaaagct ggacattttc taatagtatt tcacgagaca aaggaaatgc taagtaatca
    14461 taaaaagcac gagtgactgc tgtggcgact gcagcgcttg atcccattcc acgttcagca
    14521 ggaatcgtgc tttcaatggt cagttgcaag tttttttgga tgtcatgctg tttttttaag
    14581 tgaatgaata attttttgat attttttaat gcatgaggag cttcggctaa atttcctgaa
    14641 tagtaggaag aaaccagttg atcgtccatt gcatccagct cttcaaggaa tgcggtgact
    14701 tttgttgcat agaaaggaaa ggcaatcgct ggttctccat aaacaaccgc gtgctcgccc
    14761 atcaatatga tctttccgct tgattctcct tggccatagt ttgccataat ctaactcctt
    14821 ctcacgtaaa agtgattttt aagcaaaaaa taaagcgccc aaaacgagtt gcgctgcttc
    14881 acgaattttt ttgtccacta agggtctcaa aaaacattcc tcctttattt tactaaaacc
    14941 atctcagaag taaaactaag aatacgtttt ctaaagaaaa aacacttttt tcttaagaat
    15001 tttttgactt tttttcttct ttgactagaa aaaaatacaa ttgacactac tagatattgg
    15061 gggtatattt aaaaaggttg gagttaaaca caatatttag tgatgaagtg agctggaggt
    15121 aaatgagcgt gatcgtttta gcaggtacaa ttggtgccgg aaaatcaagt ttaacagaaa
    15181 tgatggctga acactttgat agtcaggcat tttatgaatc gattgatgat aatgaagtct
    15241 tgcccttatt ttatgcaaac cctgaacagt atgctttttt gctgcaaatt tattttttga
    15301 ataaacgttt tgcaagcatc aaacaagcaa tgaaagatga caataatgta ttggaccgtt
    15361 cgatttatga agattcactg cttttccatc taaatgcaga tctaggaaga gcaacagaaa
    15421 cagaagttcg tgtgtatgat gaactgctgg aaaacatgat ggaagaactt ccctatgctg
    15481 cccataaaaa acatccggat ttattagttc atattcgtgt atcttttgat acgatgttgg
    15541 agcgtatcga aaaacgtggt cgttcgtatg aacaattgtc ctttgatcca agtctatacg
    15601 attattacaa agaattgaac agacgttacg atcaatggta tgaagaatac aaagaaagtc
    15661 caaaaatcca aatcgatgga gaccgctata attttgtgga agatcctcaa gcaaaagaag
    15721 aagtcttgaa aatgatcgaa gaaaaattag cagagattcg ccaaaccaaa gtagccattt
    15781 gatttacagt tgctcaaata aagggtaaac ctttatttga gcgacttttt tctttatact
    15841 ggaaacaaat gtcaggagga tagagtatgc gggaaaaaca ctattttaat caagaccgta
    15901 atttttggat caatgtacag caaaacgatg tgctagctgt taaaaactta aaagaagaat
    15961 atggcattag tgacgagatg ctgacttatt ctctagataa aaatgaacgg gcgcgcgtag
    16021 agtacgattc ttttgatgaa gcgttactgc ttgtatcaaa tgttccgcac cagcaaaaag
    16081 tcgataatca ttatgaaact agtcctatcg cttttatttt aaaagaagac ggtttgttta
    16141 catttaccac gccgaatacc gagtacgtga ttcgactaat tcgatcgctt ttggaacgaa
    16201 tgccagacat gagtgtatac agtttattgt ttcgtacatt gtttttgatt tctgattcct
    16261 tttttccttt aatcgaggaa gtgaatagtg aacgtcagcg gcttaatctg aaattaagag
    16321 aaaaaacaac gaacaagaat ctgctgcaac tttcagattt agagatcggt ttggtttact
    16381 tagttacagg aacaaaacaa aatgtagtac ttttagaaca aatcaaggca ttggctattt
    16441 atcgcaagtt gtcagaaaag gaaaaagaag agttggacga tgcgctgatc gaagcacgac
    16501 aagcagttga aatgacgaac ttagcttcgc agattttaga ccagctctct ggcacttata
    16561 ataatttgct gaataacaac ttgaatgaca cgatgaaatt tttgacggtc tggtcgctga
    16621 tcttgaccgt tccaactatt gtgacaggat tcttcggaat gaatctccag ttacccttta
    16681 cccattccgt tttcggatgg ggaatcgcct tgatcatcag tttggtctta tctatttgga
    16741 tgcttattgc actgtggcgg cggattcgtt gatctagtct ttgatctgca aaccaagggc
    16801 ttgggcgatc acaagtgctt gctcttggtt gacgattagt cccgccaaag aatcttgaga
    16861 taaagcgatt gctgtgaaga aattagtcgt aaagtctaat cccttcagag aagttcgaaa
    16921 ccagttcgaa ccggtcaact gattgtcaga caaaaaaagg tttttccaag tgacctcata
    16981 aaattcgctg tcttctaatt gacaatgatc gaaggagaca gctttcaagt ttgtactact
    17041 aaaggaagcc atcgaagcaa tacaatcagt gaaagtacag tctcgaagat aactttcggc
    17101 aaaattcgta cctgttaatt tacattgatg aaacacgact tggtggaaac tgccaccaat
    17161 ccactccaga ttggaaaaat cacatttttc aaaaatgaca ttactgcatt caaaatgacg
    17221 taaaactgtt ttttgcatcg taagtttttg gatatgactc tctcttaata ccatattttt
    17281 ggcatcgaga tagctttggt cacccccgtt gatcagaaga ccagagaaga tctcctcatc
    17341 ttctaaataa aactctccct ttgtaagaag aggaagagaa ggtggaatag gttcgatttt
    17401 tttcatgtgg gttcacctcg tttttcttta gtatagcaaa gaagcgggaa tcactgctag
    17461 aaagtctgct aatctaatca ttttcagaaa aggaagacaa actgtatgaa aaacattgac
    17521 aaaaagatta gtttttgcta tactatgatg gttgagaaat aaaagcagaa gcacccgctt
    17581 ctcgccttag ttgataatgt cactgggcta gatagttttc actggttcta ttaggtgaag
    17641 tacgcgcggg ttctgtaaga gaactcgttt ttttattgcc gtttattcgg taagcttttc
    17701 tttctctcat aatatttgga ggtgaatgac catagcaaag gatatgatgg ttaacgacgg
    17761 cattcgtgca cgagagttac gattgatcgg ttcagatggt gagcaattag gagttaagac
    17821 aaaagcagaa gcattgaata ttgcagaaca agcaaacttg gatcttgtgt tagttgcccc
    17881 tggtgcgaaa ccaccagttg cgcgaatcat ggactacggt aaataccgtt tcgaacaaca
    17941 aaagaaagac cgcgaagcgc gtaaaaaaca aaaagtgatc aatgtaaaag aagttcgctt
    18001 aagtcctacg atcgacttga atgacttcaa tacaaaactt cgtaatgcac gtaagttctt
    18061 ggaaaagggc gataaagtga aagcttctat ccgtttcaaa ggccgtgcca ttacccataa
    18121 agaaattggt caaaaagtct tgaatcgctt agctgaagaa actgcggata ttgcaacagt
    18181 ggaacaaaaa gcgaaaatgg acggacgcag catgttcttg acgctggcac cgaaaaacga
    18241 cagtaagtaa tttgaatgac taactggctg ttggacagcc gatagtcgga taacgaaaga
    18301 ttttgaggag gaaatatagt catgccaaaa caaaaaacac accgcggatc agcaaaacgt
    18361 ttcaaacgta ctggtaaagg cggattgaaa cgcttccgcg cgtttacaag tcaccgtttc
    18421 cacggcaaaa caaaaaaaca acgccgtcaa ttgcgtaaag caagcatggt ttctagtggc
    18481 gacttcaaac gtattcgcca acaactagca aaaatgaaat aagaagtaca aataacagaa
    18541 tctgaagatt tatttttatt aggaggaatt atacatggca cgtgttaaag gtggaacagt
    18601 aactcgtaaa cgtcgtaaaa gagtgctaaa attagcaaaa ggttactacg gttcaaaaca
    18661 cactttattt aaaacagcaa aagaacaagt aatgaattca tacaactacg catatcgcga
    18721 tcgtcgtcaa aagaaacgcg acttccgtaa attgtggatt gctcgtatca acgcagcagc
    18781 tcgtatgaat ggcttgagct actcaaaatt aatgcacggt ttgaaagttg ctgaaatcga
    18841 catcaaccgt aaaatgttag ctgacttggc tgttaacgat gcagcagcat ttactgcatt
    18901 ggctgaccaa gcaaaagaag ctttggctaa ataagctaaa caattataaa gaggaaagaa
    18961 gataacgaaa agttatgttc tttcctcttt ttttatttac aatcctccat caaatgaaac
    19021 aggacaataa cgtataagtc taaaatagca taattaaggt tttttaattg aaaactgaac
    19081 atttatggtc accctttaat gaaagcgcta actacataag ttagaatgag tttgactgat
    19141 atattagttt gcttatcaga aaaacaaaat ggacggagga ataacatgag tgatattatt
    19201 attggtattt tgttagttat ctcattcttt ttcatggtct ggtactgtgt aaaaggctac
    19261 aacttgatgg tcggctttgc catcatggct actgtatgga tgggattagc gctagtcgga
    19321 aacacgtttt caccaaaccc ggcgatggaa gggcaaggag taattgatgt gttgacgcat
    19381 atctatacga cggggccagc tgagtacgcc aaatcgatct tggtaaatgt cttctttggt
    19441 gcctttttcg ggcgtgttct agtagatagc ggaatcgcag ctaccctgat tcgaaaggta
    19501 gttgaacttg gaggggacaa gccaagaatc acgatgagct tactatgtat tgtaaccgct
    19561 gttatattca tgtcgatgac aggaatcgga ccagtgattt ctattgcagt tattgtgttg
    19621 ccaatcttga tgtcattagg tatttcagta ccggttgcac tgttctcatt tatgggctcg
    19681 attatggctg gaatctttgc taatatcgtc aatttcaaac aatatcaaac gatttatgct
    19741 ggtttcaatc cagcggctga aagctatact tataatgact atttccaaat tggtatgatt
    19801 gggatgattg taagcttagt tgtggtacta acggtcgcta atatatcaat gaacaaaaag
    19861 aaacgctatg caatggcagc aaacgtacca gctgaaggtg gcgacgcacc aatgatctca
    19921 tggttagccg tattattacc agttttaggg gtcgttcttt tagatcttcc aatcatctta
    19981 ggttttattc tagcaggtat ctgggcattg ctgtttactg gaaaattgcg cggaggttat
    20041 aaagaaatct gtcgtcagtt tgcgaaatta tttacagatg gcgcagtaga tgttgcgcca
    20101 atggttggtt tcttgatgac cttagcgatg ttcaataatt cagctgcgta tgcttctcct
    20161 tatttttcag ctatttttgg agattggata ccgcaatcac cattagtctt ggcaatcgta
    20221 tttgctatat tgacaccact aggatttttc cgcggtccaa tgaatctagt cggttcaggc
    20281 tcagctattt tagctgtcgt attagctgtt aatccaacga tgtcgccagc ctttttgttc
    20341 ccgttatttg caatcacaac gattgctcct cagcatttag atattacaca atcttgggtt
    20401 gcttggggat taggttatac aaaagtaact tctcgagaat atatgaagaa atccatccca
    20461 actggttgga tcatcggagc gatcttatgc ctgatcacgt tcttattgta cgggaacgcc
    20521 tagtaaacaa aacgtcgata aaaacgatta gatgaatgga ggaaaagaaa atgaaacgtt
    20581 ttgtgcgaat gggaattgat gttggcggta cacatacgaa agcagtcgca atcgataatg
    20641 cgacgcacga aatcatcgga aaatcttcag tgaaaacaac ccacgatgat gtccgtggag
    20701 tagccgcagg tgtcgttcag tcttttcaaa attgtttgct ggaaaataat atcagtccag
    20761 aagatgttgt gtttgttgca cattctacca cacaagcgac taatgcactg attgaaggag
    20821 acgtagcaaa agtcggcgta atcgggatgg caaaaggtgg attagaaggt tttttagcaa
    20881 aacgacaaac tagattgaac gatatcgatt tagggaataa gaaaaaaata gagattgtca
    20941 atgcattctt acctgtcaaa cacttaaatg tagaccgtgt ttcagaaacg atttcttctt
    21001 tagaaagaga aagagcggag gtcttggttt cttctatggc atttggagta gataatgggg
    21061 aaccagaacg agttgtttat gaagcagcat cggtaaaatc gatccctaca acgatggctt
    21121 ctgacatcac aaaattatat ggattgacaa gaagaactcg gacagcagca atcaatgcat
    21181 ctattttacc aaaaatgcta gatacggcaa cctctacaga agattccgta cgagaagcag
    21241 gggtcaatgt ttctttgatg attatgcgcg gagacggcgg agtaatggaa atcaatgaaa
    21301 tgaaaaaacg acctgtttta accatgcttt ccggtccagc agcatcagtc atgggctctt
    21361 tgatgtactt gagagcgtct aatggtgttt attttgaagt aggcggaaca acaaccaata
    21421 tcggggtcat taaaaatgga cgtccagcaa tcgattattc aattgtaggt ggccacccaa
    21481 catatatctc ttcattagat gttcgggttc taggtgtggc aggcggttcg atggtacggg
    21541 ctaatcagtc aggaatcatc gatgtcggtc cacgttctgc acatattgcc ggtctagatt
    21601 atgcagtatt tacggagaca gaaaaaatca aaggtcctaa ggttgaattt ttctctccta
    21661 aagaaggaga ccctgctgat tacgtcaagg tagtgatgga agacggagaa gaagtgacta
    21721 ttacaaatac atgtgcagcc aatgtccttg gattagtgca agaagaacat ttttcttatg
    21781 gaaatgtccc atctgcacga aaagccatcc aagcattagc tgattattgt catacgacgg
    21841 ttgaagacat cgcagaacag atcatggaaa aatcctatgc taagattgaa ccggtgattt
    21901 tagaattggc agacaagtat cacttggaga aagatcagat ttctcttgta ggtgtagggg
    21961 gcggcgctgc ctcattgatt acttacttta gtaataaaat gggtgtgaaa tattcgattc
    22021 ctgaaaacgc cgaagtgatt tcttctattg gtgtagcact tgcaatggtc cgagatgttg
    22081 tagaacgtat catcccttcg ccaagtaaag aagatatacg atcgttgaaa aatgaagcga
    22141 tgaataaagc gattgaatca ggcgctacac cagaatcgat cgaagtccac gtagaaatcg
    22201 atccacagac ttcaaaagta acggctatcg ctactggttc tacggaagtg aaagcaaccg
    22261 atctaaccaa ggagatcaca acagaagaag cactagagct tgctgcagag gatatgcgat
    22321 taaataaaaa tgaagtttgc ttattggaaa atacaccttt cttttatgta tgcggcgaac
    22381 agaatcgttc aaaaaatgct ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg
    22441 tccaacgtgg acatgcttca tgtatgaaaa caactgcagc caattatatg acagctgtcg
    22501 aacaactttg ggaagatatg gcagtttatc aaactgaatt gattgcccga ccagaatttt
    22561 atctctgctt aggagcaaga gtctctgatt ttacagcgac ggatctggaa caactacagc
    22621 ttttgatgga tctggaagta tctactatgg aaccggagga agaagtgatc gtcgttgccg
    22681 gaaatatcaa acaaacataa gttagaagag ttactaaacg aattacaaca gatccccgaa
    22741 gaaatttggg gattctacca gtttcggcga gatttatttt ggaaaaagat tcctttgtcc
    22801 aagcagaaaa ttcttataca acaatcaatc gattgtggta tcgaaacggc atgtagcatc
    22861 aaaaaaaagt acccttttgc cgatgtagga gaaatttgtg aacaaatggc tgttccaatc
    22921 gtttcctgtg aatcagagca aataaacgaa cgaatcactt ttgcaactta tgcagaagat
    22981 gaagggattc gattgatgac ggagccgtta gaaaaattaa aatgttcagg actagcttcg
    23041 atctctaaag aaacagcgca agctttgatt attggacatg aactatttca ccacatcgaa
    23101 gcttctgtta agggaattta tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt
    23161 tatacacatc aatcaaacat tcgtgcattg agcgagatag cagcgatgag tttttcaaaa
    23221 gagatgaatc aaagcgattt ttcgccttat gtattagagg cagtcttact ttggccatat
    23281 aatgaaattc gtagccaagg gatcttagaa gaaataaaaa aaatagaaaa aagatgtacg
    23341 gagtatgatt tagattacaa ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt
    23401 ttccgaataa acgatgttct cctgtcaact cctcggtcac aagtcaaaat attcaacaaa
    23461 catgagaagc tgttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg
    23521 acctcttgga aaacggtgtt caaaagcttg catctgcggt aataagtcca gccaagttaa
    23581 aaacataaga agctattttt acttggctgt tcttattact tgatgcaggg cagctttttc
    23641 acaacctctc aataaacggt gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg
    23701 gcaaaatatg aggaactatt ttgccgtttc tttctctaat actcaggtca aaacgctgat
    23761 tccaaaacct cttatttgat gggtctgtcc tgaatatctt caaaataatc ggggtaatta
    23821 tgaatcacgt attcttgctc ctcgataatc atatgtaaat gcatcgtgca aagaaatccg
    23881 acttcatcaa agttatgatc aatcattgat tgaagcattg tctgatgttc atctaatact
    23941 cgaccccagt ctaattctga aatcgtcaaa cgtaaccatc tgaaacgcgt actataactt
    24001 tctagccaat cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca
    24061 gtatccaaat caaaaaaagc ttttttattt tgagcgatta ctgcctgatc ggtttgttct
    24121 agtatttttt ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg
    24181 atcggttttt ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt
    24241 tttgaaacat aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg
    24301 atcagtgctt ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg
    24361 gagattttac ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa
    24421 gcttgctgtt gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta
    24481 ttataacata aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc
    24541 ctgataggca tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc
    24601 tcttggatcg gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg
    24661 tctttgaaaa tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg
    24721 atgtaaccgt gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt
    24781 atacgaacta ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac
    24841 atcatccaaa gaagcgtacg cttggaataa ctgatctaac tccggattga ctttgctcag
    24901 cttcactaat cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt
    24961 taattttttg tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg tccccgtata
    25021 cttttggata gttggatgga tgctcacttt ttctccgtaa tggatcattt ctttatagct
    25081 gcaatttact tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa
    25141 ccctagatgt accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg
    25201 atgaataatc cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa
    25261 tttctccatg actcatgcct cgttttccat tgatttttta caaaaataat tcatccattt
    25321 cggaagtgct tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc
    25381 tccaatgaaa ctatgaagat aaatagctgc tgcaatcgtt tggtcatttt gaggaaactg
    25441 ggctaaaaat cctgtgatca tcccagctaa agtgtcaccc atgccgcccg ttgccatagc
    25501 agcagaacct aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact
    25561 tttcaaaacg agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg
    25621 ttcttgtatc ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt
    25681 ttgttcggga tagggtagca aaagatgatt agcagcaagt aaacttaaag cagagccatc
    25741 aatcaccagc cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact
    25801 cttagaagaa gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga
    25861 aagaaactgg tcagtatccg tccaaactac aatcattgct tctggcagat gactgtgaag
    25921 agcactccga tttttttcat cagttactac agtcgtgaga cccgctccgc tgtgtacggc
    25981 agctaaagca ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat
    26041 acgtccaaat gttcctttat gtgaaatttt tggacgaggt gtgatgactc ttttcactag
    26101 ttgttcgttt atcagcatct aaaaacctcc ttttactcat tatagcgtat atttttttta
    26161 gagaaaaaca gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt
    26221 gtggtatcat gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg
    26281 gcaaaaagaa gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg
    26341 tgtcaacagc gagcgcgatg actcaaaagt aacaccagat gcaccatttg gacctggacc
    26401 tcgcgacgca ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa
    26461 tgtcgataac tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt
    26521 tggacacatg gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt
    26581 aatcaaggat ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc
    26641 agcttactat gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg
    26701 cttcgtagtc ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca
    26761 tgaagaagaa ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga
    26821 aaaaggaaat gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt
    26881 gaaatctttt gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact
    26941 tcaagtacca agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga
    27001 agcaaatcca gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt
    27061 tggtaaaggc gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc
    27121 attgttctta gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc
    27181 tgcttatgtc catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa
    27241 aatgggcaaa ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga
    27301 agcgaacttc atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca
    27361 aagtgacatc gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt
    27421 aatggaacca cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta
    27481 tgaagaccac actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg
    27541 tttgttgaaa cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca
    27601 tcaagcaaac gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga
    27661 tgccatctat cgtttggcga aataattaat taaaaagagg atcaagaatt gcaaattatt
    27721 tttgcagtct tgatcctttt tttactttct actgtggtat tcatgataaa catgatatag
    27781 attcaattca tattctttct tagcgttcgt gacgactgta tccaaaatca accctgttac
    27841 taatgaaaga agtgctaaag tcatcaatcc agtacttaaa atcgcagaag ggatgcgtgt
    27901 aataaagctt gtcagcatat attcgcgaat cacaggaatc cccgtgacca atccaaataa
    27961 aaagaagaag atggtccaaa tcccaaagaa aagcaaaggc ttataatctt taaacatttt
    28021 aataatcatc atgatcactt tgaatccgtc agagaatgta ttcaattttg attcgctgcc
    28081 ttcaggacga tcacgataat cgattgggat ttcaacgaac ttgaattttt tatctaaagc
    28141 atggatcgtc aattccgttt cgatttgaaa accagaactc ataataggaa aacttttgac
    28201 aaagatacga ttgaatcctc gatagccggt catgacatca agaatttttg ttttatacat
    28261 tctagtaatt gtatttctaa caaggttatt tccaaaatcg tggaaaggtc tcttattctc
    28321 atcaaagtag gttccatttg ataggcgatc accaatgacc atatctgctt caccagaacg
    28381 taatttctcg agcaaaccat gtacagcttc tgcaggataa gtatcatcgc catctaccat
    28441 taaataatag tcggcatcga tatcaaagaa catttggcgg atcacatttc cttttccttg
    28501 acgaggttct ttttttacga ttgctccacc ttgaacggcc aattcgtatg ttcgatcggt
    28561 cgaattatta tcatagacat aaatatctgc ttctggtaat tctcgtttaa agtctgcaat
    28621 gactgttgaa attgttgctt cctcattgta acaaggaatc agaacagcaa ttttttcttg
    28681 ttgttccatt tttcactctc cttaactata gaaaacaaaa gctaaattaa aaatacctca
    28741 taatcaaagg aaatgctagt ttttcccgct aaatatcatt tatttaaaaa gaaaatactg
    28801 gaatatatgt ttggaaagct ctatctaaaa aaccacccac tacaaaagaa ggacatgata
    28861 cctctctttt gaatagtggg tgcgtattcg ttctctcact tttttgacgt attaatctgt
    28921 tgcacctttt tttcttgaaa tttctaatac ggttcctgta tgagcatctg ctaaaaattc
    28981 ataataaaca gtttcgccat cttctattcg agaaagacct ccccgataag cactcgaccg
    29041 aacggcgaat ttcctaatag gttccttttc aaaagagatc caagaacctt cgattgcacc
    29101 ttcttttaga aaagatgact tgatattttc aagtacttgg tctgctgaca atactttttt
    29161 gcgatgatac caaatcgttg aagctacccc gctcaataat cctagaccga ttccaactgc
    29221 taacccgcct ttaaaatagc ttagttcgct ttcttcatac atgtggacat acctccttct
    29281 tctcacttgt tactccttgt gcttatttta ccacactctc atattttctt tgaaataaat
    29341 aaccgctacc agaaaaaata agctataata aatacgatcg gtctgcagaa aaaatcatat
    29401 tttatccgca gatcaatata agtctgagag gagtttagtc tgaaatttag acgaatagca
    29461 tatggaagaa aaaacatttc aacgaatcaa agagttaaca gaattacaag gaacaagcgg
    29521 ttttgaacaa gatgttcgcg cttatatgga aagtcatatg gaaccgctgg tagatgagtt
    29581 acagttagat ggtttgggcg gcatttttgg tctgcgccat catgaagaag cggatgcacc
    29641 ccgtgtaatg gtcgcagcac atatggatga agtcggattc atgctgacac aaatccaaga
    29701 aaacgggctt tttaaagtcg tacctctagg cggctggaat ccttacgttg tttctgctca
    29761 gcgttttaca cttaaaacct ctactggtaa gaattatcca tgtatttctt cttccgttcc
    29821 cccacattta ttgcgaggaa caagcggaca aaaatcagta gaagtaaccg atattttgtt
    29881 cgatgctggg tttgaatcaa gagaagaggc aatgagttac ggtgtccttc caggagacac
    29941 aatcgttcct tacgctgaaa cgatcaaaac agccaacgga aagaatatta tcagtaaatc
    30001 atgggacaac cgctatggct gtacaatggt attagaagct ttagaagcgt tgcaaaatga
    30061 aacattaggt catacattga ttgcaggcgc gaatgtccag gaagaagtag gcttgcgtgg
    30121 ttctaaagct tctgtcaata aattcaagcc agatctgttc tttgcagtag actgctctgc
    30181 agcagatgat acagtgacga aaaatggcac attcggacat ttaggagaag gtacattgat
    30241 gcgtatccaa gatccaggtt tgatcatgct tcctcgtttg cgcgagtact tgctggatat
    30301 cgctgaaaca aacaatatcc cttatcaata ctttgtttca aaaggcggta cagatgcagg
    30361 tgctgctcat acacaaaacg aaggaatccc aagtacagtg attggcgtag tgggacgtta
    30421 tatccatacg catcaaacga tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt
    30481 gattcaaaca ctgaaaggtt tggataaatc aacagtaaat acgatcgtat acggaaaata
    30541 atgcgatagt gaaactataa aatctggcaa aagattgctt tttgccagat tttttttata
    30601 aaaagaggat caatgtttgt tagttcgaaa gaaaggtgta taatttaatt gaaaaataca
    30661 taaacagaaa gtagaggaca aagaatgatt atcccaaata gtttagaaga attagcaggc
    30721 tacgtagaaa ctggaaagag tgtatttttc tttactgccg gctggtgcgg agactgccgt
    30781 tttatcaaac cgcaaatgcc tgaaattgaa aatgactttt ctgattggca atttattgaa
    30841 gtagaccgtg acaagtacat agatgtagcc gctgaatgga acatttttgg tatcccaagt
    30901 tttgtcgtga tccaagacgg aaaagaatta ggtcgtctag tgaataaaga tcgtaaaaca
    30961 aaacaggaaa tcgaatcgtt cctagaaagt gtaacaaaag gctgacatct gttaggaggg
    31021 gtcaagatga atcaagaata taagaatatt ttagtaggta tcgacggtag tgaacaagca
    31081 cttgctgctt tcaaaaaagc ggtagaagtg gctcgccgta ataatgggaa agtatatgta
    31141 gcaaacgtaa tcgatcagca attttataac tttatgggct atgcacctat tgatcaaaat
    31201 ctagtcgatc agcaaactga aacagccaag gaaatgatcg aagaatgtaa agaatatggg
    31261 aaatctgttg attacacaaa tatagaagga attattgcat atggttctgt tcgtgaatcc
    31321 atggcagtca aattgccaaa aaaatatgag atcgatctaa tcatggttgg gcagtcaggt
    31381 ctaaatgcag tcgaacgctt tatgacagga agtgtagcca gctacgtgat acgtcattca
    31441 ccttgtgatg tcttgatcgt ttctgacgaa gaagaacagc caagtaatta agcaagtgga
    31501 acttgtacga cctttttaat caattaacta aaacagtaaa gggactgtga caaaagtctt
    31561 gtcacagtcc cttgttacga ataaatggtg ttccagaaga ggcttcttcg gaaataaagc
    31621 atgaaaaaaa tttcatgcag tgaatgcaat tttttgttaa gataaatctc agcaaaggat
    31681 aatggaggaa agaacatgat ttttgcttat aacaaagcac acgtcggaga caccttgatg
    31741 gttattatag ccgacgataa aggaacagaa aacactgttg aacgcaaatg gaatgttgct
    31801 cggatcaaag atgaacaagg tcaagttgtt gcttggaact ttttccatat ctccgatcat
    31861 ctaacaatcg aagggaacgg ccaagtaagc ttaacagatg aggaattagc gaaattgaat
    31921 cgattgatca aagaagcagg attcgaagaa cagcttgaag ctgatcatga accgaagatc
    31981 gttgtcgggt acgtaaaaac atgcgtccca catcctgatt ctgaccattt atcgattact
    32041 gagacagaag tagacaatgg tacagtattg caaatcgttt gcggtgcacc caatatcgaa
    32101 gctggacaaa aagttgtcgt tgcaaaacca ggagctatga tgccagatgg aatgatgatc
    32161 tggccaggaa ctttgcgagg ggtagatagc tatggaatga tctgttccgc tcgtgaattg
    32221 catttgccaa atgctccagc aaaaaaagga atcttagtat tgcctgagga tgccgtagtc
    32281 ggagaagcat ttccaaaata aagaggttgt gacaagtctt tgtcacaacc tcttttttat
    32341 taattttcac tattttgatt ttgttttaat gccgattgat cgatcgtcaa ttcaacagta
    32401 gttgtttttt ctttcgagcc ttcatagtaa gtgattttga cagaatctcc cacttttttc
    32461 ttatacaaag cagattgaag ttctacacca gaagaaactt ctttgtcatc gatcttagta
    32521 atgacgtcgt attgtttcaa tccagctttt tcagccggtg tagctgtttg gacatttgtc
    32581 acgatgacac cgttagttac agatgaagga atcttcaaga tttgttcttg ttgctgagta
    32641 gaaacggcag ataagtcgac cattgtgata cctagagctg gacgtgtcac ttttccgtct
    32701 ttttccagtt gattgatgat attcactaca tcgttacttg gaatagcaaa tcccattcct
    32761 tctacgctta cattagaagt agattcagaa gtacttgcga tcttacttga gttgataccg
    32821 ataacttgtc cttcgatatt aactaaagga ccaccagaat ttccagggtt gattgctgca
    32881 tctgtttgga tcgcattgat attgactgtt tcgttcgatt cattcgtact tgtcacttgt
    32941 cggttcaagg aagaaatgat tcctgaagtg acagaatttg catattcaga tcctagtggg
    33001 gaaccaatcg caatcgcagg ctcaccaacc ttcagagcac ttgagtcacc aaaagaggca
    33061 actgtttcga ctttgtctga attgatcttc aacaccgcta aatctgaata agcatctgtt
    33121 ccaactaatt cagcttttac cttcgtaccg tctttcatca agacttcaag accttgctgg
    33181 ccatctacta cgtgattatt cgtgactaca taagcagtat tcccgtcttt tttgtagatc
    33241 acgccgcttc cttcactata agcttccaaa ttactatcat ctgagctgct actttcttct
    33301 tgctgcccga atagtccgcc aaaaccgctt gattggtttt gactttgtaa gttgataaca
    33361 gaaacgacag cgtcttgaac tttatctacc gctttggtga tatcagaatc tacattgact
    33421 ttgacatttt ccacaactgt ttcaccggca gaattctgat tgccgcttgt agcagtagaa
    33481 ctattgttgc cagtgcccat cgctgcataa aaaatgccag ctgtcagtag tcctccaacg
    33541 atccctccaa caagccccag acctaactta cgccagaggc tattattttt actttttttc
    33601 atctttggtg tcacattttt tctatccatg tgttcctcct ccaattacat tcatttatct
    33661 actgttagta tagtcttatt tgttgacttt agtctacttt gagactgtga actttttatg
    33721 aaaaacttta ggaaaaatca ttgttttcag atgaaaatat ttgattttct ttttccacag
    33781 atactgtgta aaactacgcg gataggattg gaaaaataag ggataaaaca tatccacagc
    33841 ctgtgtataa agtggataaa ttcttataaa acgtatgttt gcctaatgaa tgtgatgaaa
    33901 taaggtaaaa taacactgtt ttctgtggat aaacctgtgg atgttgtgca taactgttgg
    33961 ggaaatcttt ttaacaaaaa tagccaaaat aaacatatag acgaaaaaat tgattataat
    34021 aaacaaagtg agcatgaaga ccaaaggaga gaaaacatga atatcaaaat tatctcagta
    34081 ggaaaattaa aagaaaaata cctgatccaa ggtatcaatg agtatgtcaa acggctgaat
    34141 gcctatgcaa aaatcgaatt gatcgaagtt cctgacgaaa aagccccgga gaatttaagc
    34201 gaagcgcaaa tgagacaagt aaaagaaaaa gaaggggaac ggatcttagc gaaaatcaaa
    34261 gaacaagaat acgtctatgc cttagcaatt gaagggaaaa atcctacaag tgaatctttt
    34321 gctaaacaaa tcgaccaatt aggtatccaa ggaaaaagcc accttgtttt tgttatcggc
    34381 ggctctttag gactcagcga agccgtcatg aaaagaagca atgctcagat ctcttttgga
    34441 aaaatgacgt atccacatca attgatgcgt ctgatccttg tcgagcaaat ctaccgtgcc
    34501 ttccggatca atgcgggggc tccataccac aaataatgat attttttaag agaaaaaatt
    34561 atccctattt taaataatag caataggtag tggtagggag aaatttataa ataacttttt
    34621 tatttgcttt ggtagccaag taagttgcaa aagaaaaata gatactgtta aaaagataat
    34681 gtagacatag aaacaaatac gtaaaatttt agttagaata atggcaagga gaaaaaacat
    34741 gatagatgaa aaaaagtgtt taaaaaatct attagaggat atggatgata ttgaagaaaa
    34801 ctatctaaat ggactgggaa aacaaatata tttcagatac ttaagctatc taaaaatgaa
    34861 ataagacata gtaatttttt agcttttttg tttgctccaa atgaaaccca caatttatct
    34921 gatgagtttt ttaaaatgtt tttgaaaagg tatatagatt ctaatgatga taccaaagct
    34981 gctatcaatt attttgatgc tttacttaat agctatgaag atttaattgt ttacagagaa
    35041 aataagaata ttgatatttt attggtatcg gaaaaaaata aaatagtggt gtgcatcgaa
    35101 aataaaattc tgtcttcgga gtctggaggg caacttaata aataccaaat atatatagaa
    35161 cgcaattatt ctaactataa aaaaattttt gtttttttaa ctccagatgg aaatgaaccc
    35221 acaaatccta gttggggcat aattacttac aagaatatcg ttgaagagag tgtaaaatat
    35281 tttgtgtaaa tagaaaaaag gaagtccctt ctgtagaata gagttaccac aacacattca
    35341 cagaaaagag gacttccata tgaacgattt tactacagaa attatggaaa tactaatcaa
    35401 taaaggtgat ttggatgatt tgtttcgtcg tcacctagaa ttggctatta acgctttatt
    35461 acaagctgaa ttaacggtat ttcttgacta tgaaaagtat gatagagctg gatttaattc
    35521 aggtaattcc cgtaatggga attattcgcg ctcttttaaa acagagtacg gtgaattgaa
    35581 tttagtaata cctagagata ggaatggaga cttttctcaa caaactttac ctgcttataa
    35641 aagaaccaat gactctttag aaaccactat tattcagcta gttcaaaaag gaattactat
    35701 gtccgaaatc tctgatctaa tcgaaaaaat gtatggtcat tattacaaac cacaaactat
    35761 ttcaaatatg agtaaaattg tgtctgaaga tgtttcggca tttaaagaaa gaactttaga
    35821 agctaaatac tcagtcattt ttatggatgc tactcatatt cccttaaaaa gacaaaccgt
    35881 atcaaaagaa gctgtctata tagtaattgg tatcaggttg gatggaacta aagaggttct
    35941 aggatttact atagctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagatct
    36001 aaaagaccgt ggtttagaag aggttttgtt agtcgtaaca gatggtttaa gtggtattga
    36061 cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc
    36121 tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa
    36181 attagtttac caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga
    36241 taaatggaaa aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac
    36301 cttttataat ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg
    36361 ttttaacaaa caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc
    36421 tctggagaga tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt
    36481 tcataaaggt tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca
    36541 gaatgggctt ttccatttac acaaaattat tgacgctccc gacaaaatta tgatcttgcg
    36601 taaagttata aaatattaat aaaaatattt tattaatgaa aagcttggta gcacggttat
    36661 tcatatgcta atttatttca ctttatagta aggggagttc ttagtatatt gttcaaactt
    36721 aaatttcata taatttaatt atgtaaatta gctattctaa gaacttctac ggataactgc
    36781 ccgtctaaaa tttaaataag agcttagaat cttcatataa gtaa
//
LOCUS       DAYYAJ010000011        35882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000011 DAYYAJ010000000
VERSION     DAYYAJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.011"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHATZ_000503"
     CDS             <1..1152
                     /locus_tag="ACHATZ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM8403061.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHATZ_000504"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHATZ_000504"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8403062.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHATZ_000505"
     CDS             2728..3003
                     /locus_tag="ACHATZ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8403063.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHATZ_000506"
     CDS             3009..3776
                     /locus_tag="ACHATZ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8403064.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHATZ_000507"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHATZ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8403065.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACHATZ_000508"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACHATZ_000508"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8403066.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACHATZ_000509"
     CDS             7920..8222
                     /locus_tag="ACHATZ_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8403067.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACHATZ_000510"
     CDS             8225..9388
                     /locus_tag="ACHATZ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8403068.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACHATZ_000511"
     CDS             9414..12842
                     /locus_tag="ACHATZ_000511"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8403069.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACHATZ_000512"
     CDS             12863..13987
                     /locus_tag="ACHATZ_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8403070.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACHATZ_000513"
     CDS             13968..14414
                     /locus_tag="ACHATZ_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8403071.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACHATZ_000514"
     CDS             14398..14736
                     /locus_tag="ACHATZ_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8403072.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHATZ_000515"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHATZ_000515"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8403073.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACHATZ_000516"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACHATZ_000516"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8403074.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACHATZ_000517"
     CDS             15893..16936
                     /locus_tag="ACHATZ_000517"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8403075.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACHATZ_000518"
     CDS             17095..17823
                     /locus_tag="ACHATZ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8403076.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACHATZ_000519"
     CDS             17939..18184
                     /locus_tag="ACHATZ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8403077.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACHATZ_000520"
     CDS             18181..18399
                     /locus_tag="ACHATZ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8403078.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACHATZ_000521"
     CDS             18535..19074
                     /locus_tag="ACHATZ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8403079.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACHATZ_000522"
     CDS             19067..21340
                     /locus_tag="ACHATZ_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8403080.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACHATZ_000523"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACHATZ_000523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8403081.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHATZ_000524"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHATZ_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8403082.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACHATZ_000525"
     CDS             complement(23167..23436)
                     /locus_tag="ACHATZ_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403083.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACHATZ_000526"
     CDS             complement(23698..23826)
                     /locus_tag="ACHATZ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403084.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACHATZ_000527"
     CDS             complement(23906..24361)
                     /locus_tag="ACHATZ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8403085.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACHATZ_000528"
     CDS             complement(24499..26427)
                     /locus_tag="ACHATZ_000528"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8403086.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACHATZ_000529"
     CDS             complement(26624..27457)
                     /locus_tag="ACHATZ_000529"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8403087.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACHATZ_000530"
     CDS             complement(27649..28353)
                     /locus_tag="ACHATZ_000530"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8403088.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACHATZ_000531"
     CDS             complement(28376..29002)
                     /locus_tag="ACHATZ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8403089.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACHATZ_000532"
     CDS             29328..31703
                     /locus_tag="ACHATZ_000532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8403090.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACHATZ_000533"
     CDS             complement(31727..32218)
                     /locus_tag="ACHATZ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8403091.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACHATZ_000534"
     CDS             32405..33325
                     /locus_tag="ACHATZ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8403092.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACHATZ_000535"
     CDS             33479..33823
                     /locus_tag="ACHATZ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8403093.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACHATZ_000536"
     CDS             complement(34013..34513)
                     /locus_tag="ACHATZ_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8403094.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACHATZ_000537"
     tRNA            35041..35124
                     /locus_tag="ACHATZ_000537"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="ACHATZ_000538"
     CDS             complement(35448..>35882)
                     /locus_tag="ACHATZ_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8403095.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAYYAJ010000012        35388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000012 DAYYAJ010000000
VERSION     DAYYAJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.012"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1529)
                     /gene="zwf"
                     /locus_tag="ACHATZ_000539"
     CDS             complement(3..1529)
                     /gene="zwf"
                     /locus_tag="ACHATZ_000539"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8403096.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(1544..2191)
                     /locus_tag="ACHATZ_000540"
     CDS             complement(1544..2191)
                     /locus_tag="ACHATZ_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8403097.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(2375..2704)
                     /locus_tag="ACHATZ_000541"
     CDS             complement(2375..2704)
                     /locus_tag="ACHATZ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8403098.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            2881..3609
                     /gene="sfsA"
                     /locus_tag="ACHATZ_000542"
     CDS             2881..3609
                     /gene="sfsA"
                     /locus_tag="ACHATZ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8403099.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            3625..4638
                     /locus_tag="ACHATZ_000543"
     CDS             3625..4638
                     /locus_tag="ACHATZ_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8403100.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            4638..5915
                     /locus_tag="ACHATZ_000544"
     CDS             4638..5915
                     /locus_tag="ACHATZ_000544"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8403101.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            5978..8680
                     /locus_tag="ACHATZ_000545"
     CDS             5978..8680
                     /locus_tag="ACHATZ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8403102.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            8832..9149
                     /locus_tag="ACHATZ_000546"
     CDS             8832..9149
                     /locus_tag="ACHATZ_000546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8403103.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            9179..9499
                     /locus_tag="ACHATZ_000547"
     CDS             9179..9499
                     /locus_tag="ACHATZ_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8403104.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            9607..11067
                     /locus_tag="ACHATZ_000548"
     CDS             9607..11067
                     /locus_tag="ACHATZ_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8403105.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            11135..11356
                     /locus_tag="ACHATZ_000549"
     CDS             11135..11356
                     /locus_tag="ACHATZ_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403106.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            11387..11569
                     /locus_tag="ACHATZ_000550"
     CDS             11387..11569
                     /locus_tag="ACHATZ_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8403107.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            11569..11982
                     /locus_tag="ACHATZ_000551"
     CDS             11569..11982
                     /locus_tag="ACHATZ_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8403108.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            12105..13286
                     /locus_tag="ACHATZ_000552"
     CDS             12105..13286
                     /locus_tag="ACHATZ_000552"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8403109.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(13357..13521)
                     /locus_tag="ACHATZ_000553"
     CDS             complement(13357..13521)
                     /locus_tag="ACHATZ_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403110.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            13675..13748
                     /locus_tag="ACHATZ_000554"
     tRNA            13675..13748
                     /locus_tag="ACHATZ_000554"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13709..13711,aa:Arg,seq:cct)
     gene            complement(13794..13907)
                     /locus_tag="ACHATZ_000555"
                     /pseudo
     CDS             complement(13794..13907)
                     /locus_tag="ACHATZ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(14290..15522)
                     /locus_tag="ACHATZ_000556"
     CDS             complement(14290..15522)
                     /locus_tag="ACHATZ_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8403111.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            15776..16345
                     /locus_tag="ACHATZ_000557"
     CDS             15776..16345
                     /locus_tag="ACHATZ_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8403112.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(16523..16963)
                     /locus_tag="ACHATZ_000558"
     CDS             complement(16523..16963)
                     /locus_tag="ACHATZ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8403113.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            17121..17885
                     /gene="map"
                     /locus_tag="ACHATZ_000559"
     CDS             17121..17885
                     /gene="map"
                     /locus_tag="ACHATZ_000559"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8403114.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            17917..18840
                     /locus_tag="ACHATZ_000560"
     CDS             17917..18840
                     /locus_tag="ACHATZ_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8403115.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            18916..20055
                     /locus_tag="ACHATZ_000561"
     CDS             18916..20055
                     /locus_tag="ACHATZ_000561"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8403116.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            20048..20848
                     /locus_tag="ACHATZ_000562"
     CDS             20048..20848
                     /locus_tag="ACHATZ_000562"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8403117.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            20848..21675
                     /locus_tag="ACHATZ_000563"
     CDS             20848..21675
                     /locus_tag="ACHATZ_000563"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8403118.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            21680..22387
                     /locus_tag="ACHATZ_000564"
     CDS             21680..22387
                     /locus_tag="ACHATZ_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8403119.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            22487..23353
                     /gene="rfbA"
                     /locus_tag="ACHATZ_000565"
     CDS             22487..23353
                     /gene="rfbA"
                     /locus_tag="ACHATZ_000565"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8403120.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            23367..23939
                     /gene="rfbC"
                     /locus_tag="ACHATZ_000566"
     CDS             23367..23939
                     /gene="rfbC"
                     /locus_tag="ACHATZ_000566"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8403121.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            23961..24989
                     /gene="rfbB"
                     /locus_tag="ACHATZ_000567"
     CDS             23961..24989
                     /gene="rfbB"
                     /locus_tag="ACHATZ_000567"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8403122.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            25087..25938
                     /gene="rfbD"
                     /locus_tag="ACHATZ_000568"
     CDS             25087..25938
                     /gene="rfbD"
                     /locus_tag="ACHATZ_000568"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8403123.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            25973..28006
                     /locus_tag="ACHATZ_000569"
     CDS             25973..28006
                     /locus_tag="ACHATZ_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403124.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            28050..29330
                     /locus_tag="ACHATZ_000570"
     CDS             28050..29330
                     /locus_tag="ACHATZ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8403125.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            29540..30346
                     /locus_tag="ACHATZ_000571"
     CDS             29540..30346
                     /locus_tag="ACHATZ_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403126.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            30358..31578
                     /locus_tag="ACHATZ_000572"
     CDS             30358..31578
                     /locus_tag="ACHATZ_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403127.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            31568..33154
                     /locus_tag="ACHATZ_000573"
     CDS             31568..33154
                     /locus_tag="ACHATZ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8403128.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            33193..35331
                     /locus_tag="ACHATZ_000574"
     CDS             33193..35331
                     /locus_tag="ACHATZ_000574"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8403129.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT        11212 a         6067 c         7356 g        10753 t
ORIGIN      
        1 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
       61 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
      121 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
      181 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
      241 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
      301 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
      361 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
      421 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
      481 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
      541 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
      601 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
      661 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
      721 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
      781 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
      841 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
      901 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
      961 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
     1021 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
     1081 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
     1141 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
     1201 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
     1261 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
     1321 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
     1381 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
     1441 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
     1501 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
     1561 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
     1621 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
     1681 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
     1741 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
     1801 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
     1861 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
     1921 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
     1981 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
     2041 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
     2101 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
     2161 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
     2221 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
     2281 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
     2341 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
     2401 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
     2461 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
     2521 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
     2581 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
     2641 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
     2701 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
     2761 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
     2821 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
     2881 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
     2941 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
     3001 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
     3061 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
     3121 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
     3181 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
     3241 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
     3301 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
     3361 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
     3421 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
     3481 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
     3541 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
     3601 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
     3661 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
     3721 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
     3781 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
     3841 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
     3901 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
     3961 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
     4021 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
     4081 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
     4141 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
     4201 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
     4261 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
     4321 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
     4381 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
     4441 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
     4501 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
     4561 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
     4621 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
     4681 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
     4741 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
     4801 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
     4861 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
     4921 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
     4981 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
     5041 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
     5101 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
     5161 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
     5221 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
     5281 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
     5341 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
     5401 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
     5461 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
     5521 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
     5581 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
     5641 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
     5701 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
     5761 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
     5821 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
     5881 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
     5941 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
     6001 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
     6061 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
     6121 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
     6181 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
     6241 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
     6301 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
     6361 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
     6421 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
     6481 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
     6541 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
     6601 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
     6661 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
     6721 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
     6781 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
     6841 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
     6901 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
     6961 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
     7021 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
     7081 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
     7141 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
     7201 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
     7261 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
     7321 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
     7381 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
     7441 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
     7501 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
     7561 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
     7621 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
     7681 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
     7741 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
     7801 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
     7861 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
     7921 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
     7981 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
     8041 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
     8101 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
     8161 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
     8221 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
     8281 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
     8341 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
     8401 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
     8461 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
     8521 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
     8581 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
     8641 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
     8701 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
     8761 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
     8821 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
     8881 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
     8941 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
     9001 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
     9061 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
     9121 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
     9181 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
     9241 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
     9301 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
     9361 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
     9421 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
     9481 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
     9541 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
     9601 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
     9661 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
     9721 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
     9781 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
     9841 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
     9901 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
     9961 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    10021 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    10081 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    10141 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    10201 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    10261 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    10321 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    10381 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    10441 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    10501 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    10561 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    10621 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    10681 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    10741 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    10801 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    10861 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    10921 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    10981 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    11041 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    11101 gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca
    11161 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    11221 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    11281 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    11341 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    11401 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    11461 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    11521 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    11581 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    11641 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    11701 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    11761 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    11821 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    11881 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    11941 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    12001 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    12061 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    12121 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    12181 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    12241 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    12301 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    12361 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    12421 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    12481 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    12541 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    12601 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    12661 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    12721 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    12781 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    12841 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    12901 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    12961 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    13021 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    13081 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    13141 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    13201 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    13261 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    13321 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    13381 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    13441 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    13501 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    13561 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    13621 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    13681 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    13741 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta
    13801 gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact
    13861 ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga
    13921 gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt
    13981 ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa
    14041 cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg
    14101 ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac
    14161 aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga
    14221 tctaagccaa tagcttagat cctttattct atgctttata ttattttttg catgtgtgcg
    14281 aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc catcttcacg
    14341 gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg tttgacttct
    14401 attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt tcacactgaa
    14461 ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata atgtattgaa
    14521 aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga cttctcctaa
    14581 acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc cttgttcggc
    14641 agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga tgatggtgcc
    14701 agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac tgtcaggtgc
    14761 tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag cattatagct
    14821 tccagcgcct tcccaaagat ggtttttagg caatccgata atgatatctg taccgggagc
    14881 agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt ttgctaattt
    14941 ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag tcgttttgaa
    15001 aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga agacttttgc
    15061 tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg cttgagttgc
    15121 cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta ctcgatggct
    15181 atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc ttgctccttt
    15241 ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa tacgatccgt
    15301 tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa cgataacctc
    15361 agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg cctgttccac
    15421 gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt ctgcaattaa
    15481 gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa ttcctcctca
    15541 ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa caatgaggct
    15601 ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat gttactactt
    15661 gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac
    15721 aatgaaatta atcaatggtg gaagcgttat aaaaaaatta aggagattaa aaaatatggc
    15781 aagaaaaaag acgattacta gagaacagat tttggcagct gcttatgaag tagctgccac
    15841 cgatggattt acacgattta ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca
    15901 accgatttat cttgagttta aaaatatgga tgacttgaga caagctcttt ttgagcagat
    15961 ctatgatcat ttacaatatg aagtgttcac tgttgagcat acagggaata cgattatcga
    16021 tcttgctttt aattatatcc attttgcaag aagagagaag aagttgtatc gttcactgta
    16081 cttagaagaa tatggtggag gaaaagagat gcagaatttc tcttatactt actttgtgaa
    16141 tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa gagcagatcg aatctttgta
    16201 taatgggaca tggatcatag ctactgggat cgcagctttg atgtcttcaa gcatcatcca
    16261 tccaactgat gaacagattg aaaagatgat ccaagacagt atagatgcta tcttgaaatt
    16321 caaagaacca atcgaaatcg attgaatata ctcaaaagtt ccacaaatca gtaggtaaca
    16381 agagatgtgt caaaataagg aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc
    16441 cgaagaactc ttccatgaat gcaatgaata aaagagccat acagattgaa taaatctgga
    16501 tggctctttt attgcaagta tattaaattt ttgcgactag ttctttagcg aaagcttcta
    16561 aattttcgat atcttcttct tcagcattca gatccacttt aacattttcg gctccttttg
    16621 atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac cgatttgcag aattcatcgt
    16681 aaaaagtatc accagatccg cagacaccgt aaatttttcc caatagatcc aattcttgca
    16741 agtcttcata aaagtctacg atctcatcag gcagatcgcc atcatcgtat gtataagtag
    16801 ctactacgca aatatctgcc tcctcgaatt catctgcatc tacttgggta cattcgttga
    16861 tttcgacttc gatatccaga ttttcaaatg cttctgcaac aatatcggcg atttcttcag
    16921 tgtttcctgt catgctagca tatacgattt ttgctaaagc catgatgtgc ctcctcgttt
    16981 ataattattt aacaattatg aattatacca aaacatcatt gaaaagaaaa taaaacctaa
    17041 agacacctgg ggaatgtcat ttggctaaat gtatgccgct atgttaaaat gagagggatt
    17101 tgtaaaaagg agacgaaaat atgattactt taaaatcagc acgtgaaatt gaaatgatgg
    17161 cagaatctgg agctttatta gcagatgtac ataaaaatct gcgtgatttt ataaaaccag
    17221 gaatcacaag ttgggatatt gaggtatttg tcagaaacta tatcgagagt catggcggaa
    17281 ttgctgcgca aattggcttt gaagggtatg aatatgcgac ttgtatcagt ataaatgatg
    17341 aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg gtaaaagtcg
    17401 atatgtgtat cgatttgaaa ggcggcattt ctgattcttg ttgggcctat gtagttggag
    17461 aatctacacc tgagatcgac cgattgatgg aagtaacgaa gaaagctttg tacatcggta
    17521 ttgagcaagc acaagtaggg aatcggattg gtgacatcgg tcatgctatc caaacttatg
    17581 tagaaggcga aaatctagcg atcgttcgtg attttattgg tcatggcgtt ggtcctacga
    17641 ttcatgaaga accagcagtt cctcattacg gagaagctgg aaaagggttg cggttgaaag
    17701 aaggcatggt tatcacgatc gaacctatgg ttaataccgg aacttggaaa atgaaaatgg
    17761 atcctaacgg ctggactgcg tatacaagag acggcggact ttcttgccaa tatgaacata
    17821 cattggctat tactaaagat ggtccaagaa ttttgacatc acaaggagaa gaaggcactt
    17881 attgatctat ccagtcaata ggtacgggag aggtacatga aagtttggaa caaaataaaa
    17941 caaaatgaaa atctgatgcg ctttattgag acgactcaaa agcgcatcac tgattcagaa
    18001 atgagtacga cctctgtcgt cgttgcttat tatttactct tgtctttatt tccattgatt
    18061 attgcttttg gtaatatttt accgtttctc cacatcgatc aagagactgt actgacctat
    18121 ttacgagaag tgattcctga gaccgtttac cagtttatcg gtcctgcaat caaagacctt
    18181 ttgacacaaa gttcaggagg cttgttatcc gtttctgcat tagcggcttt atggtctgca
    18241 agtcaaagta tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt agaaaataga
    18301 aaaaattttt tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt catgattgct
    18361 attagtgggg taactatcgt tcttgggttg ggacagctga ttttggatgc tattcagcca
    18421 atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg gccaatcaca
    18481 ttggtagctt tatttgtcat catgttcttg atttatttaa ttgtccctaa tgctcagttg
    18541 aagttgaaga cgatcattcc gggcacgatc ttcgctactg tcggctggat gctgttatca
    18601 caagtgttta gtatctatgc taggtatttt gcaacgagga tcagcggtta tcagatcata
    18661 ggaagtttta ttgtactcat gctttggctt aattttgctg ctacgatcat cattttgggc
    18721 ggaatcatca atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa aaaacgtcga
    18781 gatccaagcc gaaaatggtt caataaattg aaaactaaat tttctcataa aaaaaagtag
    18841 aaacaagagt tcttattgta aatttatatc agtaatttat aataattatg actcttaatt
    18901 aaagtaggtg cccatatgct tgtttctttt agtatggtta taagattttt tctgacaatt
    18961 ttattatcac tgatattaac acctgtattc aaaatcattt cagtacaaac aggtatggtc
    19021 gacaaaccaa atgaaagaag aataaataaa gttccaatgc cttcagcggg cggactgcct
    19081 atctttattt catttgttat atcgacttta ttcttattcc gtaaaattat ccctcaatcg
    19141 tatattgtac cgatcttgat tgcttcttta gtaatcatag tcacaggagt gctcgatgat
    19201 aaatatgagc tgacacctaa acaaaaaagt atagggatct tgattgcttc tcttattatt
    19261 tattttgtag cagatatacg tattgattcc ttcacgcttc cttttattgg atatatccaa
    19321 ctaggctggc ttagttttcc cgtaacgatt ttttggatct tcggtatcac gaatgcggtc
    19381 aatctgattg acggtttgga tgggctggcg gcagggattt cattgatcgg tttaattacg
    19441 ataggaatca ttggatattt tttcctccat gcttctacag tgtatattcc agttgttatt
    19501 ttctgtcttg tagcaagtat tatcggtttt tttccttaca atttttatcc ggcaaagatt
    19561 tatttaggcg atacaggagc tctattccta gggtttatga tggccgtcct ttcactacaa
    19621 gggctgaaga acgtgacttt tgtttcatcg atttctttgc tgatcgtcat gggagtgccg
    19681 gtaacagaca cgttttttgc tatcattagg agaaaagcaa accgtgtttc tttttctaca
    19741 gcagataaaa aacatcttca tcaccgacta atcagtttag gctttactca taaaggtgct
    19801 gtcctgacga tttatgcgat ggctttgatg ttttctttca cagctatggt catgaattat
    19861 actggaagaa tcggaacgat catattgatt attgccatgt tgtttgctgc aattttatta
    19921 tttgaattga ttggtctgat caatgagaaa catcagttta ttttaaaaac attgcgattt
    19981 ttagggaata aagagtaccg tacgcaagta aaacagaaat atttgaagaa atggaaccgt
    20041 catatagatg aataacaaat taaaccagcc gatccttatt agtattgtga cggcaaacag
    20101 tgataaaatt tttcggacac tagataattt tatttcaact gtaaaagatg aaacagcagt
    20161 aaaaatccga atttttgaca acaattcact tcccgagtac aaaaaaagac taacagagta
    20221 taggaaatat ccctacatcg atatctattt tcatgatgaa aattccggat ttggttatgg
    20281 acataatcac aatctgatag ctagcgattc tcgctatggc attatttgta acccagatat
    20341 tttagtggat gaagagacag ttacagcatt ggtcttgtta ttgaaagaac atccggaatg
    20401 tgcgatgtta gcacctaaga ttcttaatga agatggaaca actcagcacc tcatccgtga
    20461 gcagctggct gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg tcaaaaaaaa
    20521 gtttgacaaa cgactggctg tgtttgaatg ccgaggattg cctactgatg ctaatagtta
    20581 tgtacggatg atctcaggta gttttatggt agctgatatt gataaattta aagagataaa
    20641 ggggtttgat gagcgtttct tcatgtactt tgaagacaac gacttgtgtt tgagttttga
    20701 aaaagcaggt tacaagttat tgtacacacc cttttatgca gttatccatt tttacgggaa
    20761 gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg acttctatga tgaaattttt
    20821 caataagtgg gggtggcgtt ttttctgatg aaaccagaaa tattagtagt cgttgtgctt
    20881 tatcagcaac tattcagtca atctccagct tatggaccat taagcaaagc acttagcgaa
    20941 aagcaaattc agcttattat ttacgataat agtcctcaaa ggcagcaaaa tgagctgttt
    21001 gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc ctggtttagc ggtggcttat
    21061 aattttgcgt tgtctcaagc agatgaagga acacgttgtc tcgtcactct ggatcaggat
    21121 acgaggctag tcgacgatta ttttgaacta ttgcgtaaag taacctttac agatgaatgt
    21181 gttgctgcag ttccgatgat tttttctggg agccgacaag tttctcccct atattcagat
    21241 cgttatatca atcgtacagc tcaagcagta gaagttgaag gcccaacagc tgaacgaatc
    21301 atggcaatca attcaggtgt tgcctggtct gtcaaatttc taaaaaagat tggcgggttt
    21361 aatcctgctt tttctttaga ttttttggat cattggctgt tttggaaagt ggatcagttg
    21421 gaaaaaactg tggagatcct tcctgtacga atggaacatg atctttcggt tttagactat
    21481 gaaaaagttt caagtaatcg ataccgttct attttacaag cagaaaatcg attttatcat
    21541 gaatatgatc aaaggcattt atccagtcac cgacgacaat tattgttaag aacagctaaa
    21601 cagttcttga ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc atatgttaag
    21661 aattggaagg tgtgaattcg tgcgttcggt ttgtatagct acctttaatg gaggcagata
    21721 tttaagagag cagttagata gtattttatc tcagctttca gcagaagatg aagtagtcat
    21781 ttctgatgat gggtcagtag atgatacatg ggatatatta actgaatacg cagaaaaaga
    21841 taagcgaatc cagctttttt cggggcctcg tcaaggattg atcgctaatt ttggttatgc
    21901 gatccagcag acgaaaggag atctaatctt tttggcagac caagatgatg tttggttgga
    21961 gacaaaagtg aaaaagatca ctgactattt tgagcagaat ccggatcagc tagtagtagt
    22021 ttctgattta atcattgtgg acagcgagct gcaagagatc catgattcgt atttttcgtt
    22081 tcgaaattcc aaagaaggat ggcttaataa tgtgataaga agtcactata ttggtgctgg
    22141 tatggctttc cggtcttctt taaaacccga tattttgccg atcccgtcag aagtgccgat
    22201 gcatgatatg tggattggtt tgctgggagg aaagaagact gggttcctaa gagaaccgtt
    22261 aactctgtac cgaagacatg atttaaacgc gagtgagatt gaaacacatt ctaaattacg
    22321 gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa aatggtttaa
    22381 acgttgataa aagatgttta aatccttaat atttccatga caaatataac aattgatgag
    22441 ataataacaa agtgaaattt gaaatcaaaa aggagagcag atatccatga agggaatcat
    22501 tcttgctggg ggcagcggaa cacgtctgta ccctttaaca aaggcaacat cgaaacaatt
    22561 gatgccgatt tatgacaaac caatgattta ttatccaatg tctactctga tgttggcggg
    22621 aatcaaagaa attctgatta tttcaacgcc acaagataca ccacgtttca aggaattatt
    22681 tggaaacgga aatgatttag gactgcagat cgaatatgca gtacaagaaa gtccagatgg
    22741 attggcacaa gcgtttatca ttggagaaga atttattgga gatgatagtg tctgcttggt
    22801 attaggggac aatatttact atggtggcgg tctgtctaaa atgttgcaac gagcagcttc
    22861 aaaagataca ggcgcgacag tatttggcta tcatgtcaat gatccagaac gctttggtgt
    22921 agtagaattt gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg ctcagccaaa
    22981 atcaaattac gctgtaactg gcctttattt ttatgacaat gaggtaattt ctatcgctaa
    23041 aggtatcaaa ccttcagaac gtggagaact agagattacg gatgtcaata aagcatattt
    23101 ggataaagga aaactatccg tagaacttat gggacgcgga tttgcttggc ttgatacagg
    23161 aacacatgaa tcattgcttg aagcatctac ctttatcgaa acaattgaaa aacgtcagaa
    23221 cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg ataaggaaca
    23281 attgctggcc cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt tattgaattt
    23341 ggccgctgaa taaggagtcc ttacagatga aagtaacaga tacaaaatta acagatgtca
    23401 aaatcgtaga aatggacgtc tttggcgatc atcgcggttt cttcacagaa agttattcaa
    23461 aagcaaaatt tgcagaacat ggattgaaat atgactttgt tcaagacaac cattcactat
    23521 cagctgaagc tggtgtcctt cgggggcttc atttccaaaa aggagaagct gcacagacaa
    23581 aattgattcg ggtagtcact ggagcagttt tagatgtagt agtggatatc cgcaaaggta
    23641 gcccgactta tggtcaatgg gacggattta tcttatctga acataatcat cgacagttgc
    23701 ttgtacctaa aggatatgcc catggattcg ttactttgac accaaatgtg aacttccttt
    23761 ataagtgtga taattactat aatgcagaag cagatggggg catcgcattt gatgatccag
    23821 atttagcgat tgattggcca attgatccat caagagcgat tatgtcggaa aaagatcacc
    23881 gtcatccgac attaaaagaa tttgaagcag aaaatccatt tgtttacgga gaaatctaag
    23941 aattaaggag aaattttctt atgaaaaaca tcattgttac cggaggtgcc ggattcatcg
    24001 gctctaattt cgttcattac gtggtaaaca atcatccaga agtgcatgtc actgttttgg
    24061 ataaactgac ttatgcagga aacaaagaga acttggccgg acttcctagc gatcgcgtag
    24121 aattggtcgt aggagatgtt gcagatgcag aattagtgga ccgtctagta aaagaagcgg
    24181 acgcagttgt tcattacgcg gcagaatccc ataacgataa ttcattaaaa gatcctttcc
    24241 catttgtaca aacgaatatc attggtacgt atacactttt agaagcttgc cgcaagtatg
    24301 atgtacgcta tcatcacgta tctactgacg aggtttacgg cgatcttcca ttacgtgaag
    24361 acctgccagg acatggggaa ggcgaaggag aaaaattcac tgcggaaaca ccatataatc
    24421 cttcaagtcc atattcttct acaaaagctg gttcagatct gttagtaaaa gcatgggtgc
    24481 gttcttttgg cttacgtgca accatttcaa actgttcaaa caactatggt ccttatcagc
    24541 atattgaaaa atttattcct cgccaagtaa cgagtatctt aagcggtatt cgtccaaaat
    24601 tatatggcga aggaaagaat gttcgtgact ggatccatac gaatgatcat tcttcagcag
    24661 tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt gcagacggtg
    24721 aagaagataa taagaccgtg atggaattga tcttagaaat gatgggccaa ccaaaagacg
    24781 catacgacca tgtaaacgac cgcgcagggc acgatttgcg ttatgcaatc gattcgacaa
    24841 aattgcgaga agagctaggt tggcagcctg agtttacgaa cttccgtgat gggttggctg
    24901 acacgatcaa atggtacgaa gaacatgaag actggtggaa aaaagaaaaa gaggccgttg
    24961 aagcagcata tgccaaaaat ggacaataat acagaggaaa tttcactgta ttaatggaaa
    25021 aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca aggagcggat
    25081 aaccatatgt ttttattaac tggaggaaat ggacaattag gtacagaatt gcgccattta
    25141 ttagatgaaa aaaatctgcc atatgtttca acagatgcaa aagacttgga tatcacagat
    25201 gctgagaaaa caatggctta tattacagaa ttaaaacccg atgttatttt ccattgcgca
    25261 gcttacacag cagtagataa agcagaagaa gaagcaaaag aattagatga gaaaatcaat
    25321 gtcgatggca caagaaatgt agcacttgca gcaaaagctg tgaatgcaca attcgtctat
    25381 atcagtacag actatgtttt cgatggaaaa aacaaaaatg tagaatacga agtagacgac
    25441 caaacgaatc cactaaatga atatggtcgt acgaaattat taggcgaaaa agcagtagaa
    25501 gagattttag aagactatta tatcatacga acttcttggg ttttcgggat ctatggacac
    25561 aatttcgtct tcacgatgca acgcctagcg gaaacccgcg acaaactgac tgtagtaaat
    25621 gaccagtttg ggcgaccaac ttggacacgt actttggcag aatttatggt atatgtcatc
    25681 gaagaaaaag caccatttgg tatttatcat ttgtcgaacg aaaatagctg ttcttggtat
    25741 gagttcgcaa aagaaatatt gaaagataca gatgtagaag ttgcacctgt tacgtcagaa
    25801 gaatttccgc aaaaagcaac acgtccgcag tattcagtca tgagcctgaa aaagacagaa
    25861 gcgttaggtt ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct ggaagctgcc
    25921 aaaaaaatga ataagtaatt tcctttttat caaatagaat ttggagcatg atatggaaaa
    25981 acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aatcaggcag attcgaattt
    26041 ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca tcgtggtcat
    26101 cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg tgtcgcaaag
    26161 agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc ggtcagacga
    26221 atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttt cgttggtgaa
    26281 cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc ctgtaaagga
    26341 tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa gagatcgagg
    26401 actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat tcgaagtact
    26461 gatgatatta acgaagagga aaaaagctct atcgctaatc ggtgcatttg cgctgacatt
    26521 ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt tctttacttt
    26581 gggactgatg attgcttttt atcattattt ctacaatcat gataaaaaat ggctacgaac
    26641 gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga tttacccagc
    26701 ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc ttatttacta
    26761 tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg cagttttatt
    26821 tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt tagcttcttt
    26881 gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga ttggcgaatt
    26941 tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt cgaataactc
    27001 tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc catttgtgtt
    27061 atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga ttttttgtct
    27121 gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct
    27181 gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt gtttattatc
    27241 gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt ataaattcat
    27301 tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga aaatggataa
    27361 ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt
    27421 tgcattattt ggctggagac gcttgtttta cctcgctttt gtcgccttag ttatcgtatc
    27481 tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa aaacactggc
    27541 aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt ctgaagatga
    27601 attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac gcttttatcc
    27661 agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt attataatcg
    27721 gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt tagatcgtcc
    27781 agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga tcgactatgt
    27841 gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt tcacgcagct
    27901 atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg attatccaga
    27961 tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg ctagctgaga
    28021 ataatcctga aatattagta gaaaggagag tgaagataat tcaagcaatc gtacaaaaat
    28081 ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttgct gacgctagtc ttttttgttt
    28141 ggacagcagg ggtaggtttt ccagcaactt tgtttatata taacaatagt tcattactta
    28201 tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt
    28261 ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt cgggaattaa
    28321 gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt tgtttagtaa
    28381 ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc tttacggtct
    28441 tcctcggaat cacgttatat cgtgtattta cagagatcca agtaccagaa ggacaaagca
    28501 tctgggctcc aggcaattcg ctttctgata tttggatcaa tacgaataca atcggaagtt
    28561 ctttgatgac tcttgcttta ttgatcagtg gatttgccag ttcttttgaa cgatggtatg
    28621 ttcgaatcat cggtgggata gcagtcgttg cagccttttt aggtatttgg gtctgccaat
    28681 ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt ggatctttta cctaaatcgt
    28741 tgatgcgtgt cattcgtgcg ccatttattg cctacacggt tacgattctt ttagctttgc
    28801 cgatttctta tctggcggct gtctctgaaa aagtcaattt gtttaccggt cgagaagata
    28861 tttggcataa attctatcag acgctaggag aaaaatctga acaagtttta ctaggaatga
    28921 aaacatttat cttccagcga ggaaaccaat ttttaggaaa ccacaatagt tataattcta
    28981 ttttgaatat ctatggattg ataggatttg ggattgctgc tttgctgctt atccttttta
    29041 ttggtcgctt aacgttgaaa gcagatttat ccaacgggca gatgactttt atctgggcct
    29101 tttttgcggt gatgatgcag tcatttatgg aggatacatt gacttcatac cgatgggttc
    29161 cgattattta tatcctgttg gctatggccg ttcatcgata tgatgaacca gaagttacgg
    29221 aggaattcta tgttatggaa gaggcatatg acgatatgac ttatgatgat acatcttatg
    29281 aggaacaacc agatagcgct cattcttcca ggatccaacg ctatcattga taggcacaaa
    29341 atacgagaga ggctgtgaca accgtcgcag ccttttttgg tttattgcaa ttgtctagca
    29401 tcttctgtaa aatagaaacg ttagagaaaa tcttgtatca tagaaaatta agaaagaaat
    29461 aagaacgcag ctcggtacca aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa
    29521 atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaagg acatttatca
    29581 gaacagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat atgcaggatc
    29641 gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct
    29701 ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata
    29761 tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt
    29821 tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc
    29881 cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga ttggcatagc
    29941 tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt
    30001 attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt
    30061 tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc
    30121 gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc
    30181 gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga
    30241 gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc
    30301 tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg
    30361 acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct
    30421 tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat
    30481 gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa
    30541 aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc
    30601 ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc aggattcaat
    30661 cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga
    30721 gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgacatcgg cgaccatatt
    30781 tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt tgctgtagcg
    30841 atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg agatcttgaa
    30901 tttcaactaa aatgtatgga aaaatttaca gaaatcaaga actcgggaaa gacgatttta
    30961 ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta ttggttgaaa
    31021 aatggaaaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta cgaaaacttt
    31081 ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga acgcttcgca
    31141 gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaagataa aaatccagtg
    31201 gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt aaaagacgat
    31261 acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt ttatgtgtgc
    31321 ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga gaatatcttt
    31381 tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc
    31441 tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa cttgtttgtc
    31501 agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact
    31561 catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat
    31621 tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcctggaa acggccgatt
    31681 aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgtcgagc tagataagga
    31741 attgttcgat tttgtcagcg agtttgccca agatggattt tatggagata tcgaaagttt
    31801 tgaatgggca aactattatg ctggacaaac atttgattac gttttgtttg ccgatgtttt
    31861 agaacatttg gtagatcctg gaaaaacgtt gaagaaagtt agagaatttt tgaatgaaga
    31921 aggagaaatt ctaattacat tccctaacct tgcgcataat tctgtcatga ttgatttgtt
    31981 caataatcaa ttgccatggg cttcttatgg tttgctagat gaaacccata attcttttta
    32041 tactcatgat ggcttccaaa aagtttttga aaaagcagga ttatacatca atattgaaga
    32101 ttatttgtac ttagctgtag gtgatacaga attaaaatca acttatgaag aattgccgga
    32161 agctgtacgt tatgatttca aaatgcgacc atttggcgaa gtctaccaat acttcttctc
    32221 attgatgaaa catccagtag cacaaagttc cattgcgcag ccgcaaaatt ctaattatgt
    32281 gaaagtatta gaagtaatcc agcaaacgaa acaagatgaa ataattcaac aaataccatt
    32341 caataatttc acaggggaaa atgagacact aagtttccat gtatcagaat tagcagaacg
    32401 aatggtgttt cgcttcgcgc agcaacctag ctttatcgaa ttcagcgcag aagctgctgg
    32461 agaaaaactc acatttatcg attcaaatgc tgtagtcaaa acagtaaatg attgttattt
    32521 gtttgatggt aaagatttac cagagttctt tttaacggat attgcaggga aagaagtgac
    32581 tatccattgt cactatcgtt ttatcggaga attgacacca acaatgaaag aattgctgga
    32641 aacaatccgt ccaatggctg aagttacaaa gcagctttct gagaaaaatg atgaattaga
    32701 aagagaaaat cattatctta ttgagaataa tacaagatta gatcagacgt tgaaaacaac
    32761 cataaatcgc tattgcacgt tgttggaaag tgatgaatgg acaatcaaac atagattagg
    32821 gcgacgtaag aaagaaacat cgaaaaaaat ccaagaaaaa gagctttctc tatgtattga
    32881 cgaaaaaatc tgggatgcag aaacaaagat attgaaaatc atcgggtggg gaatcgctaa
    32941 ttcggatcgc caaccattgt cctataagtt atcagccgac caatcaccat tttttgaagc
    33001 aattcctgtt ttacgagaag aagtcaatca agcaaaacag ctagctgaag gagcaaaagc
    33061 aggatttgaa ctgcggatcc tttgcgagaa ggaacgttca ttcttagttg aagcagtggc
    33121 tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg
    33181 agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa
    33241 cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc
    33301 cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg
    33361 gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa ggtacgttta
    33421 tcagggaaaa tgagaggaaa agcaatctta gacttccaaa cagcaagaca tcaaaatggc
    33481 atagctgtga aattaaacgg taaatatcct tatgatgacg gcattgaatc ttcatgggaa
    33541 agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa
    33601 gcaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc agagtggatc
    33661 agtcgtcatg agaaacctga tctgaaagag caaaggaaag aagtacaagg atttgcctac
    33721 cgcccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa
    33781 tgcattgatt cggtacttga tcaaacgtat gatcactggg aactatgtat cagtgatgat
    33841 gcttctactg atccagctat ccgaaagtgt ctagaatcct atcaagcaaa agatgataga
    33901 attaaagtcg ttttccgaca agaaaatgga cacatcagtc tggcaactaa ttctgcattg
    33961 gaaatggcag aaggagagtt tatagcgctc ttagataatg atgacgaatt acctccattt
    34021 gccttatatg aagtggcaaa agtgctaaac gtacatccag aactggattt gatctacagt
    34081 gacgaagata aaatcgatgc agatgggaat cgctttgacc cgcacttcaa agcagactgg
    34141 tctcccgata cattgatggg aaataattat atctctcact taggcgtata tcggacaagc
    34201 attgttaagg aacttggcgg tttccgtaaa ggatatgaag gctctcaaga ttatgatttg
    34261 gttcttcgtg tgacagaaca aattcctgca gaacacattt accacattga ccgtgtcttg
    34321 tatcattggc ggacgatccc gggatcaact gcaagcaatg gagaagctaa aagctacatc
    34381 tatgattctg gagtgaaagc attgacagat gctctatcta gaagaaatat caagggaagc
    34441 gtacatcctg gcagaatttc tggtttttat gaaatcactt atgatgtttt gcaagaagac
    34501 ttggtcagtg tgatcattcc aactaagaat ggctatgaag atctaaaaac ttgtgtggat
    34561 tcgatcatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga taatggaagt
    34621 acagatccta aaatgcaaga attatttgca gaatacaagc atcagctgaa agaccgattt
    34681 atcgtcgaat taattgacat tccattcaat tattcacgta tcaacaatct ggcagcagaa
    34741 aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat tgaacctgac
    34801 tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa
    34861 ttattttatc cggatgatac aacgcagcat gcaggtgtat tattagggat cggtggcgtt
    34921 gcaggacatg cattgaataa ctatgatcga acacattgtg gttactttgg ccgattggtc
    34981 attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc
    35041 aatgctgtca atggatttga tgaaatatta gaagtagcat tcaacgatgt tgacttgtgc
    35101 ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtaccat
    35161 ttcgaatcta aatcaagagg ttatgaagat acaccagaaa aacaaagacg ttttgctggg
    35221 gaaatcaaaa aaatgcaaga taagtggcct tcttatattg ctcatgaccc attttataac
    35281 gataatctaa ctaaagaagg aatcggcgat ttttcgttga gacctgattg attaaaagaa
    35341 ctgtattttc atttttatta atgaaaatac agttctttta atttaatt
//
LOCUS       DAYYAJ010000013        34962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000013 DAYYAJ010000000
VERSION     DAYYAJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.013"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHATZ_000575"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHATZ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8403130.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDRAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHATZ_000576"
     CDS             complement(2020..2922)
                     /locus_tag="ACHATZ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8403131.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHATZ_000577"
     CDS             complement(2919..3611)
                     /locus_tag="ACHATZ_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8403132.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHATZ_000578"
     CDS             complement(3654..4295)
                     /locus_tag="ACHATZ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8403133.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHATZ_000579"
     CDS             complement(4333..4647)
                     /locus_tag="ACHATZ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403134.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHATZ_000580"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHATZ_000580"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8403135.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHATZ_000581"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHATZ_000581"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8403136.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHATZ_000582"
     CDS             complement(5984..6916)
                     /locus_tag="ACHATZ_000582"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8403137.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHATZ_000583"
     CDS             complement(6913..7689)
                     /locus_tag="ACHATZ_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8403138.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHATZ_000584"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHATZ_000584"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8403139.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHATZ_000585"
     CDS             complement(10933..12009)
                     /locus_tag="ACHATZ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8403140.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHATZ_000586"
     CDS             complement(12014..13294)
                     /locus_tag="ACHATZ_000586"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8403141.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHATZ_000587"
     CDS             complement(13313..14239)
                     /locus_tag="ACHATZ_000587"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8403142.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHATZ_000588"
     CDS             complement(14252..15535)
                     /locus_tag="ACHATZ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8403143.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHATZ_000589"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHATZ_000589"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8403144.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHATZ_000590"
     CDS             complement(16323..17234)
                     /locus_tag="ACHATZ_000590"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8403145.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHATZ_000591"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHATZ_000591"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8403146.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHATZ_000592"
     CDS             complement(17700..18398)
                     /locus_tag="ACHATZ_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8403147.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHATZ_000593"
     CDS             complement(18385..18771)
                     /locus_tag="ACHATZ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403148.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHATZ_000594"
     CDS             complement(18918..20588)
                     /locus_tag="ACHATZ_000594"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8403149.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHATZ_000595"
     CDS             20771..21004
                     /locus_tag="ACHATZ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8403150.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHATZ_000596"
     CDS             complement(21103..21534)
                     /locus_tag="ACHATZ_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8403151.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHATZ_000597"
     CDS             21613..22026
                     /locus_tag="ACHATZ_000597"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8403152.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHATZ_000598"
     CDS             complement(22064..23212)
                     /locus_tag="ACHATZ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8403153.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHATZ_000599"
     CDS             complement(23334..23930)
                     /locus_tag="ACHATZ_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8403154.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHATZ_000600"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHATZ_000600"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8403155.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHATZ_000601"
     CDS             27160..27591
                     /locus_tag="ACHATZ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8403156.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHATZ_000602"
     CDS             complement(27636..28985)
                     /locus_tag="ACHATZ_000602"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8403157.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHATZ_000603"
     CDS             complement(29055..30218)
                     /locus_tag="ACHATZ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8403158.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHATZ_000604"
     CDS             30347..30670
                     /locus_tag="ACHATZ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403159.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHATZ_000605"
     CDS             complement(30853..32202)
                     /locus_tag="ACHATZ_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8403160.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHATZ_000606"
     CDS             complement(32271..33224)
                     /locus_tag="ACHATZ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8403161.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHATZ_000607"
     CDS             complement(33326..34516)
                     /locus_tag="ACHATZ_000607"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8403162.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34962)
                     /locus_tag="ACHATZ_000608"
     CDS             complement(34716..>34962)
                     /locus_tag="ACHATZ_000608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403163.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9952 a         7642 c         6119 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatcgc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAYYAJ010000014        34566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000014 DAYYAJ010000000
VERSION     DAYYAJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.014"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHATZ_000609"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHATZ_000609"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8403164.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHATZ_000610"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHATZ_000610"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8403165.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHATZ_000611"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHATZ_000611"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8403166.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHATZ_000612"
     CDS             2179..2499
                     /locus_tag="ACHATZ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8403167.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHATZ_000613"
     CDS             complement(2611..3468)
                     /locus_tag="ACHATZ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8403168.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHATZ_000614"
     CDS             complement(3576..4880)
                     /locus_tag="ACHATZ_000614"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8403169.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHATZ_000615"
     CDS             complement(4877..5386)
                     /locus_tag="ACHATZ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403170.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHATZ_000616"
     CDS             complement(5561..7696)
                     /locus_tag="ACHATZ_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8403171.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHATZ_000617"
     CDS             complement(7779..9434)
                     /locus_tag="ACHATZ_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8403172.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHATZ_000618"
     CDS             complement(9436..9750)
                     /locus_tag="ACHATZ_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8403173.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHATZ_000619"
     CDS             9920..10180
                     /locus_tag="ACHATZ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8403174.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHATZ_000620"
     CDS             10194..10508
                     /locus_tag="ACHATZ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8403175.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHATZ_000621"
     CDS             complement(11238..11792)
                     /locus_tag="ACHATZ_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8403176.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHATZ_000622"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHATZ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHATZ_000623"
     CDS             complement(14153..14869)
                     /locus_tag="ACHATZ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8403177.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHATZ_000624"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHATZ_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8403178.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHATZ_000625"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHATZ_000625"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8403179.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHATZ_000626"
     CDS             complement(18339..19799)
                     /locus_tag="ACHATZ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403180.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHATZ_000627"
     CDS             complement(19789..21084)
                     /locus_tag="ACHATZ_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8403181.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHATZ_000628"
     CDS             complement(21416..22093)
                     /locus_tag="ACHATZ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8403182.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHATZ_000629"
     CDS             complement(22123..23214)
                     /locus_tag="ACHATZ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8403183.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHATZ_000630"
     CDS             complement(23315..23749)
                     /locus_tag="ACHATZ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8403184.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHATZ_000631"
     CDS             complement(24333..25790)
                     /locus_tag="ACHATZ_000631"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8403185.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHATZ_000632"
     CDS             complement(25787..27682)
                     /locus_tag="ACHATZ_000632"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8403186.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHATZ_000633"
     CDS             complement(27740..29515)
                     /locus_tag="ACHATZ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8403187.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHATZ_000634"
     CDS             complement(29565..31061)
                     /locus_tag="ACHATZ_000634"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8403188.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHATZ_000635"
     CDS             complement(31255..32877)
                     /locus_tag="ACHATZ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8403189.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHATZ_000636"
     CDS             complement(33205..34518)
                     /locus_tag="ACHATZ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8403190.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         9806 a         7477 c         5838 g        11445 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatt
//
LOCUS       DAYYAJ010000015        34391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000015 DAYYAJ010000000
VERSION     DAYYAJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.015"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..996)
                     /locus_tag="ACHATZ_000637"
     CDS             complement(259..996)
                     /locus_tag="ACHATZ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8403191.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(998..2500)
                     /locus_tag="ACHATZ_000638"
     CDS             complement(998..2500)
                     /locus_tag="ACHATZ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8403192.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHATZ_000639"
     CDS             2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHATZ_000639"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8403193.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHATZ_000640"
     CDS             4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHATZ_000640"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8403194.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7842..9107
                     /locus_tag="ACHATZ_000641"
     CDS             7842..9107
                     /locus_tag="ACHATZ_000641"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8403195.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9111..9842
                     /locus_tag="ACHATZ_000642"
     CDS             9111..9842
                     /locus_tag="ACHATZ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8403196.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHATZ_000643"
     CDS             9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHATZ_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8403197.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHATZ_000644"
     CDS             10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHATZ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8403198.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            11890..12825
                     /gene="whiA"
                     /locus_tag="ACHATZ_000645"
     CDS             11890..12825
                     /gene="whiA"
                     /locus_tag="ACHATZ_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8403199.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            13393..14049
                     /locus_tag="ACHATZ_000646"
     CDS             13393..14049
                     /locus_tag="ACHATZ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403200.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            14228..14793
                     /locus_tag="ACHATZ_000647"
                     /pseudo
     CDS             14228..14793
                     /locus_tag="ACHATZ_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15492..16013)
                     /locus_tag="ACHATZ_000648"
     CDS             complement(15492..16013)
                     /locus_tag="ACHATZ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8403201.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHATZ_000649"
     CDS             16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHATZ_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8403202.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHATZ_000650"
     CDS             20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHATZ_000650"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8403203.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            20964..22133
                     /locus_tag="ACHATZ_000651"
     CDS             20964..22133
                     /locus_tag="ACHATZ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8403204.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            22157..23512
                     /gene="glmM"
                     /locus_tag="ACHATZ_000652"
     CDS             22157..23512
                     /gene="glmM"
                     /locus_tag="ACHATZ_000652"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8403205.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHATZ_000653"
     CDS             complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHATZ_000653"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8403206.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            25799..26089
                     /locus_tag="ACHATZ_000654"
     CDS             25799..26089
                     /locus_tag="ACHATZ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403207.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26102..27127
                     /gene="sppA"
                     /locus_tag="ACHATZ_000655"
     CDS             26102..27127
                     /gene="sppA"
                     /locus_tag="ACHATZ_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8403208.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            27130..27789
                     /locus_tag="ACHATZ_000656"
     CDS             27130..27789
                     /locus_tag="ACHATZ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8403209.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            27946..28878
                     /locus_tag="ACHATZ_000657"
     CDS             27946..28878
                     /locus_tag="ACHATZ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403210.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            29032..30321
                     /gene="tig"
                     /locus_tag="ACHATZ_000658"
     CDS             29032..30321
                     /gene="tig"
                     /locus_tag="ACHATZ_000658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8403211.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            30568..31818
                     /gene="clpX"
                     /locus_tag="ACHATZ_000659"
     CDS             30568..31818
                     /gene="clpX"
                     /locus_tag="ACHATZ_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8403212.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            31901..32488
                     /gene="yihA"
                     /locus_tag="ACHATZ_000660"
     CDS             31901..32488
                     /gene="yihA"
                     /locus_tag="ACHATZ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8403213.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(32562..32717)
                     /locus_tag="ACHATZ_000661"
     CDS             complement(32562..32717)
                     /locus_tag="ACHATZ_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8403214.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            32853..34181
                     /locus_tag="ACHATZ_000662"
     CDS             32853..34181
                     /locus_tag="ACHATZ_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403215.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            34192..>34391
                     /locus_tag="ACHATZ_000663"
     CDS             34192..>34391
                     /locus_tag="ACHATZ_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403216.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSEN"
BASE COUNT        11228 a         5945 c         7435 g         9783 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
       61 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
      121 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
      181 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
      241 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
      301 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
      361 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
      421 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
      481 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
      541 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
      601 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
      661 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
      721 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
      781 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
      841 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
      901 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
      961 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     1021 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     1081 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     1141 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     1201 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     1261 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     1321 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     1381 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     1441 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     1501 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     1561 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     1621 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
     1681 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
     1741 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
     1801 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
     1861 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
     1921 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
     1981 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
     2041 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
     2101 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
     2161 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
     2221 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
     2281 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
     2341 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
     2401 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
     2461 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
     2521 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
     2581 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
     2641 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
     2701 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
     2761 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
     2821 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
     2881 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
     2941 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
     3001 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
     3061 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
     3121 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
     3181 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
     3241 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
     3301 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
     3361 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
     3421 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
     3481 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
     3541 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
     3601 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
     3661 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
     3721 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
     3781 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
     3841 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
     3901 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
     3961 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
     4021 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
     4081 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
     4141 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
     4201 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
     4261 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
     4321 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
     4381 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
     4441 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
     4501 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
     4561 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
     4621 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
     4681 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
     4741 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
     4801 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
     4861 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
     4921 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
     4981 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
     5041 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
     5101 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
     5161 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
     5221 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
     5281 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
     5341 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
     5401 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
     5461 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
     5521 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
     5581 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
     5641 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
     5701 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
     5761 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
     5821 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
     5881 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
     5941 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
     6001 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
     6061 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
     6121 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
     6181 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
     6241 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
     6301 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
     6361 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
     6421 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
     6481 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
     6541 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
     6601 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
     6661 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
     6721 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
     6781 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
     6841 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
     6901 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
     6961 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
     7021 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
     7081 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
     7141 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
     7201 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
     7261 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
     7321 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
     7381 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
     7441 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
     7501 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
     7561 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
     7621 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
     7681 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
     7741 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
     7801 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
     7861 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
     7921 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
     7981 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
     8041 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
     8101 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
     8161 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
     8221 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
     8281 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
     8341 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
     8401 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
     8461 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
     8521 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
     8581 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
     8641 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
     8701 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
     8761 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
     8821 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
     8881 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
     8941 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
     9001 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
     9061 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
     9121 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
     9181 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
     9241 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
     9301 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
     9361 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
     9421 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
     9481 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
     9541 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
     9601 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
     9661 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
     9721 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
     9781 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
     9841 aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa
     9901 agaaaaagaa aactggtaat gatacgcccg gaatgatata attttactat gcacaatgtg
     9961 agaggagttc gtaattatga cagataattt gcaattagtc atcataacag gtatgagcgg
    10021 tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa
    10081 tatgccgcct cgattgattc caaaattctg ggaattaatc aaagaatcag gcaaagtcac
    10141 aaaaatggct ttaggagtgg atttacgttc tcgttccttc tttgaagaaa tccaaaacat
    10201 gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc
    10261 agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga
    10321 cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca
    10381 agcttccgtg atcattgata cgacgacatt gacaccacga caattacgtg aaaaaatcaa
    10441 tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt
    10501 taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc
    10561 tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt
    10621 gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt
    10681 attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg
    10741 cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta
    10801 taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg
    10861 aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt
    10921 ctgccagtta tcctgaaaag tttgcgaaat caaggagcag atatcacagc agtagtgact
    10981 gttgcagatg atgggggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct
    11041 ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt
    11101 ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg
    11161 atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca attattagca
    11221 aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat
    11281 gctgtcttta aagacggaac gactgcagta ggagaatcta aaatcgcgat cgaccgtaaa
    11341 accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga
    11401 aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc
    11461 accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact
    11521 gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca
    11581 gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta
    11641 ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat
    11701 ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc
    11761 accgactttt tggaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa
    11821 gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag
    11881 gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat
    11941 cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt
    12001 gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc
    12061 tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg
    12121 aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg
    12181 atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg
    12241 ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc
    12301 aataatccgg aaacgagccg ttatcatttg gaaatatttt ctatttatga acagcataac
    12361 caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc
    12421 aatggctata tttcttattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt
    12481 ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat
    12541 tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct
    12601 tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct
    12661 gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa
    12721 ttaggggaaa tgattccctc aggtgcgata tcaaaatcgg gaattaatca ccgaattcgc
    12781 aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact
    12841 gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagttata cttgcttttc
    12901 ttccgtcaaa ctataattcc atctttactg gagtaacata atttggaaag gcttctattg
    12961 tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttgtac
    13021 ataaattttt gcttaatata gaagtcctga tatagataag atcaagtatt tatctataac
    13081 tggcctgatt aattaaaaaa ctggaaggaa ctgctcgatc atccttgaga actgctcttc
    13141 ttacttctgg tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat
    13201 tagctagtcg aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg
    13261 atttattcag tgataatcat aagttcataa atagtaattg agatgtatgg ttggaatacg
    13321 ttatacctga acttgaacta ccaagatttg gtttagaatt tgagaatcga tgtttataaa
    13381 gggagggcaa aaatgcgttt ttttacaact tttactatca ttatgattgc agtacttttt
    13441 atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt tttacttcga
    13501 gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt tattgtattt
    13561 atttggagaa ctccagcacc accattgcca gagattacat atggagaatt tccttttaga
    13621 cttgagtatg aattgaatga agaattacat gtgatagaag acacgttaat agttgaattt
    13681 gatggatttg gaatgaacga aggcataggg agatatcgta gatggacaag tcgtttagcc
    13741 agcggagaag atttagtgtt gctgttagaa gttagtgata ataagcaaat attttatttt
    13801 ccaggacctg ctaattatta catgggagat aggttgaatg gttataatca tacttttcct
    13861 agtgcttctt tcattgaaag ggaaagaggc attactagaa gggatatttt acatgacaaa
    13921 gaattattag aacaatttgg tccgcttgat caaaatacaa taaatgaaga agaattgtta
    13981 aatcaatata atataaggct agtgaattgg gaaatatctg aaccgattgt taacaatttt
    14041 ggtgattaga aaacaattac gataaataga aacaagccaa atcatcagca agtaaaagct
    14101 gatgatttgg cctttcttat acgagtagta ggaattcata gcgaatatta ttacgatcag
    14161 tagatatttt gatattaaag gagtattacg aactagtatg tgagaatgta gaggtgattt
    14221 gcgaataatg agaaatttat caaatagaaa caaaatatta atcattattg ttgttatagc
    14281 tgtgtttcat ctgggaacga atgcggtttt aagtagaata atattgggtc ctaagccacc
    14341 aagacctgcg attacccgag gggagtttga ttttagatta gaatatgaag tagatgggga
    14401 aaggattgtg atagaagata caattgttgc attatttgat ggattttctg cagatgcagg
    14461 tagcatggca tggtatcgca cgtggcgttt gcatttggcg agtgacaaga gggcacaaca
    14521 attattgtta catgagttag atgatggtcg aaaaatatat tatcattttg gaggagctgg
    14581 attttatatg gggaagttag tagcagtgta actgtaacaa ggaaagattt gccaggtctt
    14641 ggcattcgaa actccatatt ttatatcgag cgtgggagag atacatctat tgggaacagg
    14701 aaagggttaa ctcttgaaga gttatataat gactttggaa tccgtcttgt tagttgggaa
    14761 cacaatccac caatagaaaa tcgctttgaa taatcgaaat ctattaatcc atttcatcag
    14821 ccagtgaatg ttgtaatgga acaatttggt ttagtgttca aaaccattga aatactaggc
    14881 tgtgatcagc aaaaatgtat ctgaataaaa aagagagcaa ctattaaatg agaggagtta
    14941 ggagttgtta gctttatacg aatcaaagtg gcaaatacag atgcgtatgc atttgtgttt
    15001 gccatcttgc gtttggtaga cgagataggt ataagctaaa gaaacaactt gtttaaaata
    15061 aacgacctag ctcaacaaac gaccatctaa tcaactaaca gcatacataa aagcaactat
    15121 ttttgaagta gaattgaaat cgggataact taaaaagtga ttgggatact agtgtgaatc
    15181 accgtacata aatattactc gttaagggaa tttgagaaat tttgtatgaa aatttaagaa
    15241 ttagttagta tgcgcaagtc aaaaatatga ataaattatt tggtggagat aatttgctca
    15301 gcttgtcata agaaatgaga taatctggca gatgcaagct tttggacacc atttattcgg
    15361 aataaggagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    15421 tccgccgttt ataggtgttt agagcgtgag acaaaagtgt tctttacttt tgtcctacgc
    15481 tccttatttt tttattttgt ttggatcaat tcttttaatt cctctattga aatagattca
    15541 tcaaagattt ctttacccaa gatgacagtc ggaacaaatc gaatatgtgc ttgctcagct
    15601 tcgttgatga cagcttggct cagctgttcg tctttttgtt ctgtcagctt tagtttatct
    15661 actgcaaaat ccgctacttc ttggagagaa agatgtttcc actcgtcttg agtatcgaat
    15721 atttgtttga tctctttgat cgctttttga ccatcgctga ttgtgagata ccggtgcatc
    15781 acgtttcctc gctgcaagct ttctttttct ttatcgaaca atttgatcac tcgctgcaat
    15841 tgcccgcttt ggactgcttc ttctaatagc tcataagatt cttcaaacca ttgtcggcaa
    15901 tacggacaag ataggttgat gaattcgacc atttttttag gagcattcga tgaaccataa
    15961 aggattcctt tttgtgtatt tgttttcgtt gcatcaatca ctgatatatc catgttaaaa
    16021 cacctctttc gcttatagtt tatacatgtc tgacaaaaaa aggcaacgat ttagactgtt
    16081 tgtctaaaaa ttagatgacc aattcaaata gacatatgtg aaatattttt ataaataaaa
    16141 gaaaaaatgg aatgttttac gttataatgg aacagataat aagagagaat atgaacaagg
    16201 atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg catatatttc
    16261 ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga ccatggctga
    16321 attagtagat caatggtcag cagcaggaaa agaaaatgta ttcggtcaac cagtaaaagt
    16381 agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat tgaaaactgg
    16441 tgcactgaca actacatata ccgcttctca agggctgctt ttgatgatac cgaatatgta
    16501 caagattgct ggggaactac tgccaagtgt tttccatgtt gctagtcggg cagtgacgac
    16561 aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac aaacaggatt
    16621 tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg ttgcccatct
    16681 tgcttctatt gaaggagaag tacctttttt gaatttcttt gatggattcc gtacgagtca
    16741 cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga tcaatcagga
    16801 gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg ttagtggtac
    16861 gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct attatgataa
    16921 attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag gaacttctta
    16981 tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt ctatgggatc
    17041 agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac ggaaaacagg
    17101 ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc aaaagttgcc
    17161 tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg caggtggaga
    17221 acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc cggctgttat
    17281 tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct cagcagtttt
    17341 tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag ggatcgtgga
    17401 tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag aaccgcaaac
    17461 attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa ataaatcagc
    17521 tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt attatgattc
    17581 gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc cgatacgttc
    17641 agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt acctgcatag
    17701 ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata ctctttggag
    17761 cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg ctgaaaataa
    17821 cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt tgggacgccg
    17881 gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc cttttgatga
    17941 ggtgcttccg ctgctaaaag aagaagccct gaaaagctat ggacataaat caatggctat
    18001 cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc aagtcgcgat
    18061 cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga acactggtta
    18121 tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct ctgttggtac
    18181 acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt ctatggaaaa
    18241 gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga tgtgtaatga
    18301 atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg aagaggaaat
    18361 ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg gcgtaaaata
    18421 ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag aggcttgtcc
    18481 cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac aagctgtgaa
    18541 ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa agaatactgt
    18601 cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct caggctgtgg
    18661 cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga tgatcgcgaa
    18721 tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt atacgaccaa
    18781 tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg ctgaatttgg
    18841 ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt caatcaaaga
    18901 agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt tggtacacat
    18961 gtttgagagc gaaggaacgc agcaaagagc agcaaaactg caagctgcga tcgaaagcga
    19021 acgatcaatt gtccctgaac tggaggatat atacaagaga aaggatctat tcgtaaaaaa
    19081 cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg gcgggatcga
    19141 tcatgtactt gccagcggag cagacgtgaa tattcttgtt ttggataacg aagtctattc
    19201 caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta aattttcggc
    19261 tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat atggaaatgt
    19321 ttatgtggcg caaattgctt caggtgccaa tcaaatgcag acgataaagg cttttgaaga
    19381 agcagaaaag tatcctggac cttctttaat cattgcctat acgccttgta tcacacatgg
    19441 tttgcttggt ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt cttctggcta
    19501 ttggtcatta tatcgatata accctgcaat ggtcgaaaaa aacaagaacc caatgacttt
    19561 ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac aagttcgctt
    19621 ttcctcgcta gagaacgcaa atccaaatct tgcaaaagct ttatttgaaa agactatcca
    19681 agacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag aaaaaatcaa
    19741 gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa aagaaaaaac
    19801 gacggacctt gaagcagaag aaagaagagc tgccagagca gaacgacgta aaaaaatgga
    19861 acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat aattctctga
    19921 agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt tcctgaaaat
    19981 tatgcgtttt tctcaagaaa aatacgagca gatcagcctc taaaattaaa acatatgata
    20041 tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca aatcggggaa
    20101 ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc tctgaatttt
    20161 ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact gatccaactg
    20221 ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat tgttatccga
    20281 atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca agtcattacg
    20341 tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg actggaacat
    20401 cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga tgagaagatg
    20461 atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg tgcgctaatt
    20521 actatcgaac ggcatactgg attagatgag tatattgaga caggaattgc attagatgca
    20581 gatatcactg gagagctgct gatcaatatc tttattccta atacaccgct gcatgacggt
    20641 gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc tctatcagaa
    20701 agtatgttga ttccaaaaga attcgggact cgacaccggg cagctgtcgg tataagcgaa
    20761 gtcagcgacg cgattaccat cgttgtctct gaagaaactg gggatgtcag cattacatta
    20821 gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatttt gcgtagagaa
    20881 ctgattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt
    20941 atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc attctttaca
    21001 gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc
    21061 caaatacgct ttctggaaat gaagcgtata atcaaacagt tactggggtg ccaatcaaga
    21121 tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg actgtcaaac
    21181 tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca cggatgttcc
    21241 gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaggttccg ctaaaaattc
    21301 aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca gtaacggttg
    21361 agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat
    21421 ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc actacaggtg
    21481 acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg atggtcacag
    21541 atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa
    21601 gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag ccaagcaagt
    21661 cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa agctatgatt
    21721 tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt gcatcgatcg
    21781 gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa
    21841 ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt gtggagatta
    21901 cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg acacaagaaa
    21961 atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca tcgacaacaa
    22021 ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca gaagaaacat
    22081 cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat taagttaaag
    22141 gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa
    22201 caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag
    22261 ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata caagaatctc
    22321 aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta tcgaagtgtt
    22381 ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc aaaaagcaag
    22441 cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa tcaaattttt
    22501 cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag cattattaga
    22561 tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg tagaagaatt
    22621 tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg gtgacctttc
    22681 cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt
    22741 gtttgccgat cttgaaacag atttttatac catgggcaca aatccagacg ggttaaatat
    22801 caatgatggc gtaggctcta cccatccaga acgtttagct gagatggtcg ttgaaaaagg
    22861 agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg tggatgaact
    22921 tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctagatact tggcagaaaa
    22981 aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag gattccacaa
    23041 agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc gttacgttgt
    23101 agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc acatgatttt
    23161 cttagatttt aacacaactg gagatgggat gctttcagga attcagttac tgaatatcat
    23221 gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct atccgcaaaa
    23281 attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa
    23341 agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag ttcgtccttc
    23401 cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga aagttaatta
    23461 ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt aagagatatt
    23521 ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt ccttgttctt
    23581 ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt cgaatccccg
    23641 taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta gagtagttag
    23701 ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc cctctgagac
    23761 aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc gagtatgcgc
    23821 accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga tcaaagcaat
    23881 cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac tgatttcttt
    23941 caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa tgtaaaaggc
    24001 atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt tttgggcgat
    24061 gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga atacaagaga
    24121 agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta cagctatttg
    24181 tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca atagcagtgt
    24241 aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa gtgaagggta
    24301 gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc cactttgact
    24361 caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa attcactggc
    24421 taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac ctgctaaccc
    24481 cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag acaatgtatc
    24541 gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt gagcgatacg
    24601 acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat aggtaccttt
    24661 ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc
    24721 aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat cttcaatctc
    24781 aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca cattgaatcc
    24841 atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa catcaggtgc
    24901 tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag cttttaaaaa
    24961 cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga taatctcagt
    25021 atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt cttcaaagtt
    25081 ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat gtggatgggc
    25141 gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac ctatatttcc
    25201 tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg ctttgaccag
    25261 ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac ggtattctaa
    25321 tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca ttccaacaat
    25381 tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct tccagctgca
    25441 ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt agagtgcagt
    25501 ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg tttacacaga
    25561 cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc tggaaacatt
    25621 taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa aataaattgg
    25681 tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg gtttttatct
    25741 atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag gagtaaaaat
    25801 ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt ccaaattcga
    25861 agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct gggatagcat
    25921 ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta cggtagaaga
    25981 tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag agcggttcga
    26041 cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag
    26101 aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg tggtttcgtt
    26161 agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat tgaacagttg
    26221 gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca gtacgcaaaa
    26281 aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag gtttatttgc
    26341 ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg aagaagatcc
    26401 aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc
    26461 ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa tgtatgtcag
    26521 catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag ataaaatctt
    26581 tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat tgaattattc
    26641 aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga
    26701 tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc aagcatatat
    26761 cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata aatctgaaga
    26821 agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa aagaagtagg
    26881 tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa aggaacaaca
    26941 gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg ctagtacatg
    27001 gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta gtcagatctt
    27061 atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt attacggagg
    27121 tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga taagcaacag
    27181 cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc ttttgaaaga
    27241 gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa gtatacggac
    27301 acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc aggttttttt
    27361 atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca
    27421 cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct gagtatctac
    27481 ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa attcaatcat
    27541 ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga tgaagaagaa
    27601 cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct aaaatttcct
    27661 ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca tctaggcgat
    27721 tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa ccaggaagtc
    27781 aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa agtcagatct
    27841 aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt ttttgttttg
    27901 ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa gccgattgat
    27961 tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc aaaccagcaa
    28021 tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag ctttgaggct
    28081 aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc attgaagcaa
    28141 gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt ttattttgac
    28201 ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag tacagatttt
    28261 tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga attatccgga
    28321 caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact tccttcgttg
    28381 gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta tcatgagttt
    28441 atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc aagggcaaaa
    28501 ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt gacgattgaa
    28561 gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca ggcaagttac
    28621 cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct tagttccgtg
    28681 ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga tacatacatc
    28741 gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa aacgatgtat
    28801 gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa
    28861 atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg aaaaacaagg
    28921 attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt aaaggtatgc
    28981 atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta aatgactgca
    29041 acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc gcaagacgaa
    29101 atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga
    29161 ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga agaagcttta
    29221 tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt caaagaagct
    29281 ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac
    29341 tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca atataaagat
    29401 ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc acgtatcaaa
    29461 cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc tgaaaatggc
    29521 gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa
    29581 ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt tgaagatcaa
    29641 ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc agaagattat
    29701 caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa
    29761 gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga cgaagtatca
    29821 acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa agaaaaagca
    29881 gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt
    29941 gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga attcttgaac
    30001 aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg ttctacagaa
    30061 gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg
    30121 atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat caatgcagaa
    30181 atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca
    30241 gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca
    30301 gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa gctacttcta
    30361 gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt ccttattttt
    30421 tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa cactttttct
    30481 ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt ttcttgcata
    30541 ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa tgagaccgta
    30601 cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg
    30661 gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga agaattttat
    30721 gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct tgaagtattg
    30781 aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc cgtatacaat
    30841 cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga actgcaaaaa
    30901 agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc acaaacatta
    30961 gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac agaagctggt
    31021 tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc tgattacaat
    31081 gtggaacgtg cagaaaaagg gatcatctat attgatgaga tcgataaaat tgcgcgtaaa
    31141 agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg
    31201 aaaattctag aaggaactgt tgcaagcgtg ccacctcagg gaggacgtaa acatccacat
    31261 caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg tgcatttgat
    31321 ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa
    31381 aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga agatttacta
    31441 aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc tttagagaaa
    31501 ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct agtcaaacaa
    31561 tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga agcgttgaga
    31621 gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg ttccatcatt
    31681 gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg
    31741 attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta taaagattca
    31801 gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc ttttgcgaca
    31861 gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc atcaagctga
    31921 aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc cagaaatagc
    31981 tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga ttgatcgaaa
    32041 gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt tttatttgat
    32101 cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag tatccaaaac
    32161 tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag accagctacg
    32221 tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata tccagatgta
    32281 tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg cagataagat
    32341 ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg attttgatcg
    32401 caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc
    32461 agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca gtcaaactct
    32521 ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg tttcttgtct
    32581 tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc ttttggtgca
    32641 actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt atctgtacgt
    32701 tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc tatcatgtaa
    32761 tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcgtt ggttataatt
    32821 aaattataaa aagattttaa agaggtgaga aaatgattgg cgaagataaa ttattgatga
    32881 ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc gattgggcta
    32941 ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa caagcggatc
    33001 cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga gacgaaagtt
    33061 ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat aatcaattat
    33121 tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg attcgagact
    33181 tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct tatagcgagt
    33241 ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa aaggatgaaa
    33301 tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat tgcaaccaat
    33361 ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg gaaatgtatt
    33421 atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc caacaagtag
    33481 aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga gaccccttta
    33541 actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag cttgggtttg
    33601 atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt gaatacattt
    33661 acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa cctgttccta
    33721 aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat tcaacaagga gattataaag
    33781 aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt gacgagaatc
    33841 gctcgcgcat gatgtgctat aaagttatcg atccgactgt tgctttggat aatggaatcg
    33901 aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat gaaaaatatc
    33961 aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca gaattaccgt
    34021 taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag aaaaagcggc
    34081 gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa tttttagatt
    34141 accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa aatggataaa
    34201 caactctatt atcgaataat ggttccggca tcattattat ccgtctgtct cttttttgtt
    34261 cctaattttc ctattcgatc agtcatcggt tttttggtca tgtttggcgg gatgggactt
    34321 tattattatc ttcttcataa ggaaagaaaa cctgcttctg tttatatgaa aaaaacaaga
    34381 agcgaaaata a
//
LOCUS       DAYYAJ010000016        32717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000016 DAYYAJ010000000
VERSION     DAYYAJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.016"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACHATZ_000664"
     CDS             complement(1..120)
                     /locus_tag="ACHATZ_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8403217.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACHATZ_000665"
     CDS             299..1960
                     /locus_tag="ACHATZ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8403218.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2392)
                     /locus_tag="ACHATZ_000666"
     CDS             complement(2030..2392)
                     /locus_tag="ACHATZ_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8403219.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHATZ_000667"
     CDS             2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHATZ_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8403220.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="ACHATZ_000668"
     CDS             3402..4445
                     /locus_tag="ACHATZ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403221.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="ACHATZ_000669"
     CDS             4451..5521
                     /locus_tag="ACHATZ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403222.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="ACHATZ_000670"
     CDS             5511..6464
                     /locus_tag="ACHATZ_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403223.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="ACHATZ_000671"
     CDS             6476..7486
                     /locus_tag="ACHATZ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8403224.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="ACHATZ_000672"
     CDS             complement(7771..8592)
                     /locus_tag="ACHATZ_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8403225.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="ACHATZ_000673"
     CDS             complement(8689..8967)
                     /locus_tag="ACHATZ_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403226.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="ACHATZ_000674"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="ACHATZ_000674"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8403227.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="ACHATZ_000675"
     CDS             complement(11723..12310)
                     /locus_tag="ACHATZ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403228.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="ACHATZ_000676"
     CDS             12485..13807
                     /locus_tag="ACHATZ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8403229.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="ACHATZ_000677"
     CDS             13962..14738
                     /locus_tag="ACHATZ_000677"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8403230.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHATZ_000678"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHATZ_000678"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8403231.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHATZ_000679"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHATZ_000679"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8403232.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="ACHATZ_000680"
     CDS             16628..17257
                     /locus_tag="ACHATZ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403233.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /locus_tag="ACHATZ_000681"
     CDS             complement(17381..18490)
                     /locus_tag="ACHATZ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403234.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHATZ_000682"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHATZ_000682"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8403235.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19308..19406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19444..19980
                     /locus_tag="ACHATZ_000683"
     CDS             19444..19980
                     /locus_tag="ACHATZ_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8403236.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="ACHATZ_000684"
     CDS             complement(20020..21783)
                     /locus_tag="ACHATZ_000684"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403237.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="ACHATZ_000685"
     CDS             complement(21786..23501)
                     /locus_tag="ACHATZ_000685"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403238.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24280..24504)
                     /locus_tag="ACHATZ_000686"
     CDS             complement(24280..24504)
                     /locus_tag="ACHATZ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403239.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24695..27472
                     /locus_tag="ACHATZ_000687"
     CDS             24695..27472
                     /locus_tag="ACHATZ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8403240.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27553..27972
                     /locus_tag="ACHATZ_000688"
     CDS             27553..27972
                     /locus_tag="ACHATZ_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8403241.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27984..28454
                     /locus_tag="ACHATZ_000689"
     CDS             27984..28454
                     /locus_tag="ACHATZ_000689"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8403242.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28471..29250
                     /locus_tag="ACHATZ_000690"
     CDS             28471..29250
                     /locus_tag="ACHATZ_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8403243.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29237..30058
                     /locus_tag="ACHATZ_000691"
     CDS             29237..30058
                     /locus_tag="ACHATZ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8403244.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30075..31124
                     /locus_tag="ACHATZ_000692"
     CDS             30075..31124
                     /locus_tag="ACHATZ_000692"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8403245.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31137..32141
                     /locus_tag="ACHATZ_000693"
     CDS             31137..32141
                     /locus_tag="ACHATZ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8403246.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT        10668 a         5818 c         6655 g         9576 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt cattcatagg
    23701 aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata tatcaagtaa
    23761 atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa tcttttttgc
    23821 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
    23881 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
    23941 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
    24001 tcccgacatc aagtcaaaag ccacttgaga ttttgatctg aaaataaaaa aagaaaacaa
    24061 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
    24121 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
    24181 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
    24241 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
    24301 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
    24361 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
    24421 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
    24481 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
    24541 aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga
    24601 taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg
    24661 acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat
    24721 tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt
    24781 tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt
    24841 gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt
    24901 ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat
    24961 cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat
    25021 gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta
    25081 tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt
    25141 agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt
    25201 catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt
    25261 tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc
    25321 agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga
    25381 aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc
    25441 acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt
    25501 aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag
    25561 tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg
    25621 gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg
    25681 aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca
    25741 gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca
    25801 gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga
    25861 gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat
    25921 cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca
    25981 agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga
    26041 gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta
    26101 tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag
    26161 cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca
    26221 acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag
    26281 taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga cgatctatct
    26341 aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg
    26401 ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt
    26461 tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga
    26521 ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct
    26581 gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa
    26641 tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga
    26701 agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga
    26761 ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca
    26821 cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata
    26881 tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt
    26941 cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga
    27001 agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa
    27061 tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt
    27121 actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat
    27181 gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta
    27241 tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca
    27301 aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt
    27361 tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta
    27421 tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc
    27481 aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta agtgaaaaga
    27541 ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt tgcttcaggg
    27601 atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt attcgattgc
    27661 tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat gaggtacaaa
    27721 gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa cgaattctta
    27781 caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc actattgatt
    27841 gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca gcaggtgcta
    27901 actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa aatagaagaa
    27961 gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat cggttattac
    28021 acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt ttgattgcta
    28081 atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc aagccacaag
    28141 gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa agcggggtga
    28201 cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat aagctggcag
    28261 aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa cctggtactc
    28321 gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg aaacgattaa
    28381 ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa gtggatgtaa
    28441 gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt attaggaatt
    28501 gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc tcgtccgatt
    28561 gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat cattatgggc
    28621 gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat tccacctgat
    28681 gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc cggtacagaa
    28741 accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa aaatatttac
    28801 ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga agaaggaaat
    28861 tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt gatgcttggt
    28921 gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct attaaatatg
    28981 attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc agctcttggt
    29041 tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt tttcttaggt
    29101 tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt tggtgcgatt
    29161 ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca aacaactgca
    29221 ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa cgaaaaaaga
    29281 gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt atgaacgtca
    29341 gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat atcagtcgaa
    29401 agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga ccccacatat
    29461 cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg atgatcctga
    29521 atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc ttgcagggtt
    29581 aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg gtacctcttt
    29641 agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca atattccgca
    29701 cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg gttttttgaa
    29761 aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta ttattggact
    29821 gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt taaaaatcgg
    29881 ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc caaatatctt
    29941 atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga aaccattaac
    30001 gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga ttttttaaaa
    30061 aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag gaagtactaa
    30121 gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa acagtgaatt
    30181 tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag ccagcattgg
    30241 aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc aattatggaa
    30301 aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa agtgcttcta
    30361 ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct atcacaaatg
    30421 acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt gccggggtag
    30481 aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta ctactcttag
    30541 ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa gactacatgc
    30601 aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg gaagaatttt
    30661 ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt ggatatggtc
    30721 caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc cgcgttccgt
    30781 cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca gatgcttcgc
    30841 atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa aagttgcaaa
    30901 gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa gcaagaaacg
    30961 agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta gcgcttgtcg
    31021 ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac ttgaacaaaa
    31081 agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg gaaatcatgc
    31141 tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct ttacgtccgc
    31201 aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc tattatctag
    31261 gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat gggaaaagca
    31321 acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac aaaagattaa
    31381 caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa gtagtgaaag
    31441 cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat aaagcgaaca
    31501 gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga acagaacaaa
    31561 tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc gaagcttatt
    31621 cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa gcagagaaaa
    31681 aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt atgcattatt
    31741 ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt tactgggaag
    31801 aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac gggacgttag
    31861 agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa cagagagtgt
    31921 tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca ttgatagaca
    31981 caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt ttgtctttct
    32041 tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg aactgccatc
    32101 cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg gataaaaaga
    32161 atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa atattggcat
    32221 caattacgtt ataaaacaca ttttttgcat tatctacagt tcaacttata atctgtttaa
    32281 tgggacatgt gggtgccaac cattttttgt tatgcaatag tactatttag ttgttgcatg
    32341 gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt ttacatagcg ttttgtatcc
    32401 agaatgttga ctaaaacaaa agttcctatg tcaaccgtca acagagacaa tctgtccgtt
    32461 gcaggacctg acagatttag aaatgttcaa tctgtctctt atgtatttta aatcaatact
    32521 cactaggaga agtttcctaa cctcctaata gtaatgctgg tagtaacatt gtcaaaactg
    32581 cttcctctta ggaacaagca aagcataacg agtgtaaaaa taaaatgtag actattgtaa
    32641 catatcattg attatttcaa gtaaaaacga taaaagaagt aaacaaacac ttaaaaacaa
    32701 tggaaattag tcccaac
//
LOCUS       DAYYAJ010000017        32704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000017 DAYYAJ010000000
VERSION     DAYYAJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.017"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..1168)
                     /locus_tag="ACHATZ_000694"
     CDS             complement(242..1168)
                     /locus_tag="ACHATZ_000694"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8403247.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(1253..2014)
                     /gene="pflA"
                     /locus_tag="ACHATZ_000695"
     CDS             complement(1253..2014)
                     /gene="pflA"
                     /locus_tag="ACHATZ_000695"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8403248.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(2258..4489)
                     /gene="pflB"
                     /locus_tag="ACHATZ_000696"
     CDS             complement(2258..4489)
                     /gene="pflB"
                     /locus_tag="ACHATZ_000696"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8403249.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(4766..7216)
                     /gene="parC"
                     /locus_tag="ACHATZ_000697"
     CDS             complement(4766..7216)
                     /gene="parC"
                     /locus_tag="ACHATZ_000697"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8403250.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(7228..9273)
                     /gene="parE"
                     /locus_tag="ACHATZ_000698"
     CDS             complement(7228..9273)
                     /gene="parE"
                     /locus_tag="ACHATZ_000698"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8403251.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(9439..9873)
                     /locus_tag="ACHATZ_000699"
     CDS             complement(9439..9873)
                     /locus_tag="ACHATZ_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8403252.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            10000..10638
                     /gene="plsY"
                     /locus_tag="ACHATZ_000700"
     CDS             10000..10638
                     /gene="plsY"
                     /locus_tag="ACHATZ_000700"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8403253.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(10770..11645)
                     /locus_tag="ACHATZ_000701"
     CDS             complement(10770..11645)
                     /locus_tag="ACHATZ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8403254.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(11727..12518)
                     /gene="codY"
                     /locus_tag="ACHATZ_000702"
     CDS             complement(11727..12518)
                     /gene="codY"
                     /locus_tag="ACHATZ_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8403255.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(12536..13936)
                     /gene="hslU"
                     /locus_tag="ACHATZ_000703"
     CDS             complement(12536..13936)
                     /gene="hslU"
                     /locus_tag="ACHATZ_000703"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8403256.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(13950..14498)
                     /gene="hslV"
                     /locus_tag="ACHATZ_000704"
     CDS             complement(13950..14498)
                     /gene="hslV"
                     /locus_tag="ACHATZ_000704"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8403257.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(14535..15440)
                     /gene="xerC"
                     /locus_tag="ACHATZ_000705"
     CDS             complement(14535..15440)
                     /gene="xerC"
                     /locus_tag="ACHATZ_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8403258.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(15638..17014)
                     /gene="trmFO"
                     /locus_tag="ACHATZ_000706"
     CDS             complement(15638..17014)
                     /gene="trmFO"
                     /locus_tag="ACHATZ_000706"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8403259.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(16983..19061)
                     /gene="topA"
                     /locus_tag="ACHATZ_000707"
     CDS             complement(16983..19061)
                     /gene="topA"
                     /locus_tag="ACHATZ_000707"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8403260.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(19183..20040)
                     /gene="dprA"
                     /locus_tag="ACHATZ_000708"
     CDS             complement(19183..20040)
                     /gene="dprA"
                     /locus_tag="ACHATZ_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8403261.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(20096..20863)
                     /locus_tag="ACHATZ_000709"
     CDS             complement(20096..20863)
                     /locus_tag="ACHATZ_000709"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8403262.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(20856..21716)
                     /gene="ylqF"
                     /locus_tag="ACHATZ_000710"
     CDS             complement(20856..21716)
                     /gene="ylqF"
                     /locus_tag="ACHATZ_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8403263.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            complement(21810..22769)
                     /locus_tag="ACHATZ_000711"
     CDS             complement(21810..22769)
                     /locus_tag="ACHATZ_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8403264.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            23041..23439
                     /locus_tag="ACHATZ_000712"
     CDS             23041..23439
                     /locus_tag="ACHATZ_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8403265.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            23676..24218
                     /locus_tag="ACHATZ_000713"
     CDS             23676..24218
                     /locus_tag="ACHATZ_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8403266.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(24301..24852)
                     /gene="lepB"
                     /locus_tag="ACHATZ_000714"
     CDS             complement(24301..24852)
                     /gene="lepB"
                     /locus_tag="ACHATZ_000714"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8403267.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25004..25231)
                     /locus_tag="ACHATZ_000715"
     CDS             complement(25004..25231)
                     /locus_tag="ACHATZ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8403268.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25228..25746)
                     /gene="msrA"
                     /locus_tag="ACHATZ_000716"
     CDS             complement(25228..25746)
                     /gene="msrA"
                     /locus_tag="ACHATZ_000716"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8403269.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(25766..26479)
                     /locus_tag="ACHATZ_000717"
     CDS             complement(25766..26479)
                     /locus_tag="ACHATZ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8403270.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(26482..27378)
                     /locus_tag="ACHATZ_000718"
     CDS             complement(26482..27378)
                     /locus_tag="ACHATZ_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8403271.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(27536..28378)
                     /locus_tag="ACHATZ_000719"
     CDS             complement(27536..28378)
                     /locus_tag="ACHATZ_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8403272.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            28510..29163
                     /locus_tag="ACHATZ_000720"
     CDS             28510..29163
                     /locus_tag="ACHATZ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8403273.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29226..29753)
                     /locus_tag="ACHATZ_000721"
     CDS             complement(29226..29753)
                     /locus_tag="ACHATZ_000721"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8403274.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(29773..30720)
                     /locus_tag="ACHATZ_000722"
     CDS             complement(29773..30720)
                     /locus_tag="ACHATZ_000722"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8403275.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(30732..32615)
                     /locus_tag="ACHATZ_000723"
     CDS             complement(30732..32615)
                     /locus_tag="ACHATZ_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8403276.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         8611 a         6882 c         5686 g        11525 t
ORIGIN      
        1 aagcggatca tcggtcagtt tttgataaaa gttttcctta ttttttgaat aatagttcat
       61 atatgttctc ttttctatcc gttttatttt acttaaaagg cttttacgta aaaaggagga
      121 agaacaagag tcataattga ctgttattct cctccatttc ttcggccttt agtatgttct
      181 atgtttcttt ttatatcaat cgcttatcca ttcattttga atgcttcata ctaccatttg
      241 attagctaaa tacagccgtc aattgaggta cgacttgttt tttacgtgaa acgacacctt
      301 taagcaaagc acggttgttg gtcaaagttg tttggaaagc ttcttctact ttttcttttc
      361 ctttaccagc aacaagtaat tctgaatcac tgtctaagat gtttgtgaca actaatacga
      421 acagatcgta gccatttgct ttcatttcat cttccattgc tgcttctagc tctgcttgtc
      481 gatccatcac ttcattaagg tccactgtat tgacttgagc gatacgaacg ttggaactgc
      541 ccattgggaa actttttgca tccaaatcaa gaagaaccgc agctgattta tcacttagat
      601 ttgttcctgc tttcaacatt tcaagtccat aagtgtttag atcgacttca gcgattgctg
      661 caagttcttt tgcagctgct tcatcttcag gcgtacatgt tggtgatttg aacaacaatg
      721 tatctgaaat gatagctgag agcatgattc ctgcgatttc tttaggaatt gttacttggt
      781 tttctttata tagttttaaa ataatcgtac ttgtacatcc aactggttct gcacgatagt
      841 acaatggatt agctgtttca aagtttgcaa tacgatggtg gtctactaca gctaaaatat
      901 ttacttcgct gatatctgaa atactttgtt ggaattcatt atgatccaca agcatgacat
      961 ctgcattttc tgctttagct gactctacta ctcgtggtgc agatagctga aagtggtcta
     1021 atgcgtattg tgtttcttca ttaggtgtac ctagagcaac tgcttctgtt tctttaccta
     1081 attcattttg caagtgtgaa aacgcaatgg ctgccccgat tgcatctgta tctggatttt
     1141 ggtgaccaaa aactaaaatt tttgacatgt cattcgctcc tttgtgattt ctcctattta
     1201 ataatagcaa aaaaacggaa agaagcaata cttatcttct ttccgatgcg acctatcgcg
     1261 ttaaatattt tgtatactct tctgtatgaa gcaatcgttt agcattttcg acacgttctt
     1321 ttgtaggtgg ttcgatccct tctaatggat atttcaaacc aagtgtttcc cacttgtatt
     1381 ttcccatcgt atgataagga agaatctcta ctctgtctac atttgacaag gtttgaataa
     1441 agttgtctag tcggatcaaa tattcatcat aatcacttcg ctcaggaact agtacatggc
     1501 gaatccaaac cggtttgttg atttttgaca aatattgagc catttctaaa atattttcat
     1561 tgtcccaatg cgttagtttt ttatgctctt cattatcgat gtgtttgatg tcaaataaca
     1621 gtaaatctgt atacttcatc aattcttgaa aacgtgaaaa gaaaggttct tcatacgtaa
     1681 aaggttttcc gcatgtatcc aatgtagtat gcatcttccg ctcttttgct tttttaaata
     1741 gttcgatcaa aaaatcgatc tgcaatagag gttctccacc actaacagta ataccacctt
     1801 tatctcccca gtaaggtctg aagcgttctg ctttatctaa aagttcatct gctgtgtatt
     1861 ctttccctcc gccaatattc caagtatctg gattgtgaca aaattggcaa cgcatgcggc
     1921 atccttgcat aaacacgaca aagcgcagtc caggtccgtc tactgaaccg aaagtttcga
     1981 tagaatgaac atagccgatt gttttttctt ccatttaagt tcacatcttt tctttgattt
     2041 cattttaagt acaagttagc tgtcgaagcg aagctttctg aacaaaattt ttcgcaattg
     2101 ccgcttcgtt atgtgtaaaa tttacaggga cggaacatac aagtcctgcc cctgtaaatc
     2161 gtttctttca aataagcata ctgattattc gattttgatg cgatggctat tcaacgtcct
     2221 ttcgacgcta agtcgaaaaa cttttttcac gaacttttta cattctgtcg tgtgaagttc
     2281 tcatcaagac atctaattgt tgttctcttg tcaagtcacg gaatttaact gcataaccag
     2341 atacccggat cgtcaagttc ggatatttca ttggattttc cattgcatca cgcaataaat
     2401 cattactgaa tacattgaca ttcaaatgat aagctccttt tgagaagtat ccatctaaga
     2461 cattacgcaa gttttcgatt cttgtatctt gttcttttcc aagacctgaa ggattgattg
     2521 tttgggtatt tgaaattcca tccaatgcac agccgtaatt cattttagct gttgagttca
     2581 ggctagctaa taacccactt tgttccgcat tgtaacttgg atttgctcct ggcgcaagtg
     2641 gttttccagc ttcacggcca tcaggagtat ttcctgtatt tttaccgtaa acaacatttg
     2701 aagtaatcgt caataatgaa gttgttggtt ttgagttgcg gtaagtttta tgacgtttga
     2761 tttgtcccat gaagtattcc aagatccatg aaactaattc atctgcacgg tcatcatcat
     2821 ttccgtattt agggaagtcg ccatccactt caaaatcaac tgctattcct ttttcattac
     2881 ggattgcttt tacttttgca tatttgatcg ctgacaagct gtctgccgca tgagagatcc
     2941 cagcaatacc tgtagcaaat gtgcgttgca agtcagaatc catcaaagcc agctgagctg
     3001 cttcataaga atatttatca tgcatataat gaatgatgtt caacgtattg acatatagtt
     3061 ctgctaacca atccatcata ttcatgaagt tatgtttcac ttcttcaaag tctagtattt
     3121 ctgatgtgat tggacgatat tctggtccaa cttgggcgcc agtcacttca tcgactccgc
     3181 cattgatggc atataatagt gttttagcca agttagcacg agcgccaaag aattgcatgt
     3241 cttttccgac aactgttcct gatacacagc aagcaatcgc acaatcatca gaaccccatt
     3301 gttcacgaag ctgattatca ttttcgaatt gaatcgaaga actttcgata gcgattctgc
     3361 tagcatatgt tctgaatcct tctggaaggt gttcagaata caaaacagtc aagtttggtt
     3421 ctggtgaagg tcccatattt gttaatgtat gaagcagacg gaaatcattc tttgtaacta
     3481 atgaacgacc atctaatccc atacctgcaa tggataaagt agcccagata gggaatcctg
     3541 agaataattg gttatattca ggtgtacgcg cgaatttgac catacgtaat ttcattacta
     3601 agtggtcgat caattcttgt gcttcttttt ctgtgattac tccattatcc aaatcacgtt
     3661 ggatatagat atcaaggaat gctgagatac gtccgattga catcgcagca ccattttgtg
     3721 atttgattgc tgctaaatat ccgaagtata gccattggac agcttctttt gcatttgcag
     3781 ctggtttaga aatatcatag ccatatttcg cagccatttc tttcatttgc gctaatgcac
     3841 gatattgttc agtgatttct tcacgtaaac gaatcacttc ttcactcatt gtatgataac
     3901 cagtcatatc atgatctttt ttcttttgtt ccatcaaata gtcgattcca tacaatgcca
     3961 cacgacgata gtcaccgatg atacgtccgc gaccgtaagc gtctggaagc cctgtaatga
     4021 ttttgttttt acgagcagca cgcatttcag cagtataagc gtcaaataca ccttgattat
     4081 gtgttttgcg ccagtcagtg aaaatatgtg tcaattcatc gtttggtgta tagccattgc
     4141 tttttagcgc attgttagcc atattgattc cgccgaacgg catgaatgct tgtttcaaag
     4201 gtttgtctgt ctgtagaccg acgatttttt ccaaatcttt atccaaatag cctgcatcgt
     4261 gtgaagtaat agttgatacg atttctgtgt ccatatcata gacaccgtta cgctcgaatt
     4321 ggatatcatt taacttttgc aattcatccc ataatttttc tgttgcttct gttgggcctt
     4381 ccaaaaagct gtcatcaccg cgatattcag tgtagttatt ttgaataaag tctctagtgt
     4441 ctacgccttc tttccatttg gtacctttaa atccattcca ttgctccatt gtatggacct
     4501 cctaaatttc tgttttatgt aacagttttt acgtgattag tataacacag caccctatct
     4561 ttgcaagggt tttcttgttt tttatttaac aatttttatg ttttatttac atatatttaa
     4621 atcaacgttg acataccaac atttaagcat tgtgaacact ttcatctttt tttgcaaacg
     4681 tataaggatt tattaaaaat gaatgaaaaa gagacgccac ctgtattaat acagatggcg
     4741 tctcttcatt aaaatactac aacagttacg actcgatgaa aatctcagga gcatcgataa
     4801 cttcgatgac ttctccacct tttttctcat ccatcgcaaa tgatccattc gatgttcgat
     4861 cacctatagg gaaatcagtt gggtcaatcg ttaactgctg ccctttttga ttcatcaagg
     4921 ttatttcttt tgatcttcct tccgacatat agataatacg gtgcggattt tttttcagct
     4981 cacgcatgac cgtcactccc cgcttcgcac gtgaagtttg gctgatttct tgagcaagca
     5041 tgcgtttaac agctcctcgc tgagtaacga ttacgatcgg cgtatcacct tctgctaaca
     5101 caagcagccc gttgataatc ctatctccat ctttcaaatt catagacttc actccagctg
     5161 cctttgctcc aacaacggga acatctgtta aaggatagcg caaacccatt ccttgatgcg
     5221 ttactaagaa aacatctaag cctggctgat cttctttcag atacacagca atcagttcat
     5281 cttcttccgc ctttagcttc atacagctga gcggacggct cttataggtg cgccatggtt
     5341 cgaactctgt cattcgtgtt tgcttgatca atccattctt gctgatgaat acaaatgttt
     5401 tctctgcttg aattttttga tatgggaaaa cagctaaaat cgattcgtct acagacagat
     5461 tggagattgt ttgagaaatg tgctcaccag catctttcca tttcaaatcg ggtagttcat
     5521 ggacagggcg gtagatcaca tttgctttat ttgtgatcag aagaacatgg tccaatgtac
     5581 tcaattcacc tgagtatagc aagtagtcgc cttctcgcat cccgatttct tctggtttgg
     5641 aagcactata agaacgaagg cttgttcgct tcacatagcc ttctcgcgtc acactgacaa
     5701 ttacgtcttc ttgtgccact aagacttgcg tatcaatttt gatttcttcg atttcgtctt
     5761 cgattgtcgt tagacgagcg gaagaataat tttttttcac ttctcgcaat tcttttttca
     5821 taacagagaa aagctctttg tcatttgaaa ggattttatt caactctgtg atttgcgcaa
     5881 tcaagctttc ggattccttt tgaagctctg tgatatccgt gttcgtcaaa cggtataatt
     5941 gcaatgtgac aattgcttct gcttgttctt ctgtaaattg aaagacgtcg actaaattct
     6001 ttttggcgtc ttttttgtct ttactttcac gaatcgttgc gattacttca tccagaatcg
     6061 ataaagcttt catcaatcct tctacgatat gctgtctttt ttgagctttg gctaaatcga
     6121 attgactacg ttttgttatg acttgctttc gatgctcgat ataacttctt aaaatgtctt
     6181 tcagtccaac ttgatgcgga gtcatatggt cgatagcgac catattgaaa ttgtaattga
     6241 tctgtagctc agtgtttttg aacaaataat tcaaaattcc ttgagcattg gcgtcttttt
     6301 tcaattcgac gacaatttgc agacctgttc tatcactttc atctcgtact tcagctattc
     6361 catcgatttt cttattcaaa cggatctcat ccattttttt caccaaggtc gctttattta
     6421 cttcatatgg gatttctgtg atcacgattt gctgtttatt acccttcatt gcctctatct
     6481 ttgttttcga acgaagaata acttttcctc gtcctgtttc ataagctttt ttgatttcat
     6541 cttttccttg caagatccct cctgttggaa agtcaggtcc aggaatatat tccatcaatt
     6601 tttcaaggct cgcttgtgga tgatcgatca tatagattgt gccatcgatc acttcagcta
     6661 agttatgagt cgggatttct gttgcatatc cagcagatat acctgtcgat ccattgacga
     6721 gaaggtttgg gtatcgagct ggaaggactg ttggttcttt ttctgtatca tcaaaattcc
     6781 acacgagatc gactgtttct ttctcgatat ctgctagcat ctctccactt aacttggata
     6841 atctggcttc agtataacgc atcgcagcag gtggatcacc gtccatactc ccgttattcc
     6901 cgtgcatttc gatcagtact tcccgcaact tccagtcttg gctcatgcgg accatcgctt
     6961 cataaatact gatgtcacca tgaggatgat aattccccat gatatttccg acagattttg
     7021 ctgatttacg gaatcctttg tcatacgtat tgccatcttt gttcatggca aataatatcc
     7081 ggcgttgcac tggcttcaat ccatcacgga tatcaggcag tgcacgttct tggataatat
     7141 atttggaata acgtccgaag cggtcaccca tcacttcttc taaagttaat tcttgtactt
     7201 cttgtcgatt ttccatcagg tgacctccta ttctacttca aataactgat tgttttcatt
     7261 tttatctgct cgttcttcat ctaataaatc attagaaact gaaggagaga tttcagtatc
     7321 ctctttttta tctaagatac tgccatcttc ttcgagcgta aattgcacgt gattttcgat
     7381 ccattttctt cgaggttcta ctttgtcacc catcagtgtg gttactcgac gttcagcttg
     7441 cgcagcatca tcgatacgga cgcggatcaa cgtacgggaa gtcggatcca tcgttgtttc
     7501 ccacaattgt tccgcattca tctctccaag tcctttatat cgctgcagca tatatccttt
     7561 gccgactttt ttaatcattg ctgccaattc gtcatctgtc cacgcatatt caatgacttg
     7621 cttctttcct tgtcctttgg atactttata taaaggcggt aacgcgatat atactttccc
     7681 agcttcaatc agcggtttca tataacgata aaagaaagta agcagtaata cttgaatatg
     7741 ggcgccgtct gtatccgcat cggtcatgat gattactttg tcgtaattac agtcttcgat
     7801 agaaaactca ggaccgactc ctgcaccaat cgtatagatc atcgtattga tttcttcatt
     7861 tttcaagata tcttgcattt tagctttttc ggtattgatg acttttcctc gtaaaggtag
     7921 aattgcttgg aactttctgt ctcttccttg tttagcagat ccgccagcag aatccccttc
     7981 gactaaatac aattcatttt tcttagggtt tcgggattgt gcgggtgtca attttcctga
     8041 aagaagagat tcgccttttt tacgcttctt cccatttcgg ctttcttctc tggctttacg
     8101 tgctgcttct ctcgcttctc tggctttgat tgctttacgg accaacatct ggctcatttc
     8161 gctattttcc tgcaaataaa agcccatttg ttcactgatg acgttatcta ctacggtacg
     8221 agcaactggc gtaccaagtt tttcttttgt ttgcccttca aattgcagaa gattttcagg
     8281 tacacggata gaaagtacag cagctaaccc ttctctgaaa tcactacctt ccagattctt
     8341 gtcccgttct ttcagcaaac caacttttct cgcatattca ttataagatt tcgtcatcgc
     8401 cgctttcata ccagcttcat gggttccccc atcttttgtc cgaacgttat tgacaaaaga
     8461 taaaacattt tctgaataac cgtcattgta ttgataagcg acctctactt cgataccttc
     8521 tttttctcct gaaaaataaa caactggtgt caatgtatct ttttcttcat tcagataatc
     8581 aacaaattct ttgattcctt cttcgtaatg aaaaatttcc ttgactggtt cttctccacg
     8641 aagatcagaa agagagattt taacgccttt tagaagaaag gcagattctc ttaaccgttc
     8701 ggctaaaatt tcatatgaaa atttcgttgt actgaatatt gtatcatctg gtaaaaaatg
     8761 gacagtcgtc ccgtttgctt tttttgtttt tcctatcttt ttcaaagtac cgtcaggttt
     8821 accgccgttt ttaaatttct gctgatactc tacgccatct ctaacgatcg ttacaattag
     8881 ccatttcgat aaggcattta ccacactggc tcctacaccg tgcagtccac cagaagtttt
     8941 gtaccctcct tgaccaaact ttccgccagc atgcagcaca gtaaaaatca cttcaactgt
     9001 tggtattccg gaagcatgca ttcctacagg catcccacgt ccgctatctg caacagttat
     9061 actattgtct tcatggatgg tcacatcaat ctcactgcca taacctgata atgcttcgtc
     9121 aactgcatta tcgacgattt catagactaa atgatgcaag cctcggctgt cagtggatcc
     9181 aatgtacatc cctggtcttt ttctaactgc ctctaatcct tccaatactt ggatggacga
     9241 gtcattgtat tcgttgttta ctttttttgc caaggcaaac gctccttcaa taatactttg
     9301 cggcaaaacc gcaccacgat tatcatacta gaaaaggtcg tgaaaaaaaa gagggtagac
     9361 gagaaatcaa ttttaattat ctcctaaccg ctttttcgac attcccctct atcgatcatc
     9421 tcttctttgt ggtcattttt atttttggtt ttctttcaac ttaccaagtt caatcttaat
     9481 acaacgatcc attacgatat ctgtacgtcc tgcttttctt agcatctcag ctgcttcttt
     9541 gttttcaatc cctaattgtg cccagaacac tttagcatca gttttcaaaa aatcacctgc
     9601 tatttcaggt aaaaattcac tccgtctaaa tacatctacg atatcgattg gatcatcgat
     9661 ttctgtcaaa ttcgcataga ctttctcgcc tagaatttct tcaccagcca ataatggatt
     9721 tactggtatg attttatatc cattgtcttg caatacctgt gcaaccatgt aacttgttcg
     9781 ttctggttta ttgctcaagc ctacgacggc aatatttttt gcttcttcca aatagttgaa
     9841 aatcgtttct tgtgttggat tttttacaga catttctgcc accccttgct tttcttttta
     9901 ttccatctat tatacttgta tctttcattt tttcaattgt tcctgttacc tattcatgct
     9961 aaaataaagg atagttttca acttgaaaga gggtaaaaaa tgaaattgat cttattactt
    10021 attttagcat atcttttagg ttctattcct tccggtgtct ggattggcaa attgtttttc
    10081 aaaaaagata tccgacaaca cgggagcggt aatactggaa cgaccaatac atttcgtgta
    10141 ttaggcaaaa cagccgggat tgttgtcctg cttatggata ttttaaaagg tacattggct
    10201 acttgtttgc cgatgatctt ccacctttct gggattaacc ctctttggtt tggggtatgc
    10261 gccattcttg gccatacatt tccgattttt gctgggttca aaggaggaaa agctgttgcg
    10321 acaagtgcag gaatgctttt aggatttaat cctgtatttt ttgtttattc ttgtatcatc
    10381 tttatcgtta gtttattttg tacaagtatg gtgagtttga gcagtatgat cagtgcagta
    10441 ttgatcactt tatctacgat cgtccttcct tatgtagctc ctgtgatttt ggctaagccc
    10501 aattggttac tgacgatcat tgccttttta gtttcttcct ttatctttta tcggcataaa
    10561 gacaatatca agcgtatcag aaaccatact gaaagtcgaa tcccgtttgg tttgaatagc
    10621 agcaagaaaa aatcctgata ctttttcatt ccaaagagat aaaacgggag tgtgacaaat
    10681 cgatgtcaca ctcccttatt acttacagga tagctttttt acacaccttg gtaaacggtg
    10741 ttccctgcta acttctcggt cacaacctct tacttcactg tgatggaata ttttgtcttg
    10801 aagatttcat gggcacctag ttgattgatc cctacttttt ccatcaagtt tccactactg
    10861 ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa cgaaaggcgc ttcttttgga
    10921 taaggagacc agatccctac ataaggcata tctttatagc ttaaagttac gctatgggaa
    10981 gatttttcac ttcgaatggc gtacgcattt agaccccttg tttcaaaaac caaggcatct
    11041 tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg tctgacctaa cgtcttttga
    11101 tccatatcga tgaatgggcc tttcaatggt aggcgtaatc ttgatttcaa tggagaaaat
    11161 gccagataga aatcttcaaa tgacaatcct tcttcaagag gtacattgaa agcaggatgt
    11221 ccaccgatag aaaagaacat ttctttttca ccggtatttg ccacttgata atgaaccgtg
    11281 atgccgtctc ctcctaattc atagctgatc actaattcaa aatcaaatgg atacttttct
    11341 aatgtctccg gcgtactcct taagatgaaa ctagccattt cttgtccatg ttcaatgact
    11401 tcaaaatcca tatcccgagc aaatccgtgt tgccccattt gatatgtttt cccttgataa
    11461 acatactgat catcttttaa acgtccaaca aaagggaata aaaccggtgc atgtctgccc
    11521 caatacgctg gatcaccttg ccagatatac tctaaaccat gttcttttga tttcagactg
    11581 atcaattccg caccgtgttc agcgatttcg gcgatcagct ggtcattttg aataataaca
    11641 gtcattgcca ttcctccaac ttcgacttac tttttaaaat gggaaggctg gaacaaacat
    11701 ttctatctgc cccagtcccc ttacttctaa taactgtgtt ttgctaactc gtctttaaaa
    11761 cgggaattca atacttttaa ataagttccc ttcatgccta acgaacgaga ttcaatgatt
    11821 ccagctgatt ccaattttcg taaagcattg acgatcacag aacgagtgat gccgatacta
    11881 tcggcaatat tagaagcagt cagacgacct tcatctccat ccaatgcttc aaagatagct
    11941 tgtacagcct tcaattcgct ataagataat gtgttgattg ccatttggac agccgttgca
    12001 ctccgtacat ctgcttcgat gcttcgagac ttctgataaa ggatctgcat accgacaacc
    12061 gtagctccat attctgctaa gactagatcg tcatctgcaa acgattgttc catacgagat
    12121 aagataatcg tacccaaacg ttcgccagct ccaaagatcg gaacgacagt cgtcagacca
    12181 aatggatatt tctctcttga ctctaccgga aatgcagtaa tatcactagt aattgggata
    12241 ttagcttcag ttttcatcaa ctgtgccact gtatcagtat aagattcagg aaattgtttc
    12301 tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt gacgttcatt ataacctagt
    12361 aatactccgt cacttcctat gatataggtg ttgctgtcaa gaataccacc tagtgtgaca
    12421 gccattttat cgtaaggcaa atctgcttgc atgtcaaaag tattcttttg ctgcaaaagt
    12481 gtgtttatct tgcgggtttt cgttagcaag tctgtcattg tcgtcctccg attttttata
    12541 aaatatagcg acttaaatct tcatttgccg caattgagcc aagtttgtca tccacatatg
    12601 cttctgtgat cgttatttct cccatttgca tatctggtgc ttcaaataat agatcttcta
    12661 gtagtttttc caaaatggta tgcagtctgc gtgcgccgat attatctgtg tcacggttga
    12721 catcgaaagc gatttgagcg atccgttcaa ttgcttcttt tgtaaacgta actgacacat
    12781 tttctgtacc gatcaacgcg acgtattgtt ttactaaagc attatttggt tcggtcagaa
    12841 tcttcacaaa atcttcagct gtcaaatcat ctaattctac ccgtatcggg aagcgtcctt
    12901 gaagttctgg aattagatca cttggtttag acaaatggaa agctcctgaa gcaatgaata
    12961 ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac ttgtgaacct tcgacaattg
    13021 gcaatatgtc tctttggacg ccttctctgg atacttctcc agattgctgt gatttagaag
    13081 tgatcttatc gatttcatcg ataaaaataa ttccactttc ttccgctaat ttgattgcct
    13141 cactatggat atcagcatcc ttgactaatt ttgctgattc ttccttgatc aataactcta
    13201 aagcttcttt gactgttacc gtccgttcca cttttttctt cggtgtcaaa gcacctagag
    13261 tttcatttag gtcgatcccc atttgctcca atccattgtt catcattggt gcaggtttct
    13321 tttggtcttc aacttcaatg gttacttcac gactatccag taaacccttt tgtagttgtt
    13381 ccaagactgt acggcgattg attcgaatat cttctgtcac ttcttcttgc ggttcagcgg
    13441 tattttgctg actagcatta aaaagattca tcatttgttc aaattgatta gtggattgtt
    13501 tctgttcttt tttgatacca ggtaccaaga tttttaccaa acgtttttct gcattttttt
    13561 tagcacttgt gtatacacgg ctgtactgct ctttttctac gatttggatc gcattttcta
    13621 ctaaatcccg aaccattgat tctacatctc ttccgacata accgacttca gtaaattttg
    13681 ttgcttcgac tttgacaaat ggtgcattga cgatttttgc caaacgtcgt gcgatttctg
    13741 ttttaccgac ccctgtaggt ccgatcatca gcatattttt tggtgtaata tcttgttgca
    13801 ttttttcatc taactgcaaa cggcgatagc ggttacgcaa agcaacggca acagattttt
    13861 ttgctgcgtg ctgtcctacg atatattcat ctaattcact tacgatttgt cttggtgttt
    13921 gttttaattc atacatatct actcacctct tacagttctt ctacgataat attatgatta
    13981 gtaaagatac agatatctgc ggcaatattt aacgcatttt ctgcaatttc tttcgcactc
    14041 atgtcgtttt gagcatagtt tttcattgct cgggctgcag ccaacgcaaa attccctcct
    14101 gatccgatag ctaagatacc atcatctggt gtgataactt ctcctgtacc tgaaacaaga
    14161 agcatttctt tatcattcat tacgatcagc atcgcttcaa gtttttgcat agattgctgt
    14221 gtccgccatt cttgagctaa ttcgacagct gcgcgttgta gattcccgtt atattcgttt
    14281 agtttgcctt caaatttttc ttctaaagta aacgcatctg caacacttcc ggcgaaacct
    14341 acaacaactt ctccattgta gatacgtcgt actttctttg cagtgccttt catgacaaca
    14401 gattcaccca ttgttacttg gccatctcca gccatagcaa atttcccgtc tttttcgacg
    14461 gcacagatag tggttgaatg aaatggtgat tcgaccatgt ttgtttcctc ctaaatttgt
    14521 tcttttcctt atttttacgc tcttggatgg aaagaacgat agtttttttg taaactttct
    14581 tttgttacat gggcatagat ctgggtagtc gataaattgg catgtcctag caattcttgg
    14641 actgtccgca aatctgcccc attatttaat aaatgagtag caaatgtatg gcgcagcatg
    14701 tgaggatgga tatcactatc caaactgctt tttttgatca tttgattcaa gacatattca
    14761 attccagtag gcgtgattgg ttttccatga tgattcacaa aaacgatatc atgctcttcg
    14821 tgatattttg tcatcaatac ctggcggcct tccctgaaat attcttgcag agcatcttga
    14881 gcaaaagaac ctagaggagc atatctctct ttgttccctt taccatggat caacatgacc
    14941 tctgaatcaa aatcaatgtc tgataccgac aaactgctac actcacttaa acggatgcct
    15001 gacccgtaaa ggacttccag taaagcgcgg ttcctcaaat ccaaaggact atctcctgct
    15061 acgctgtcaa aaagtgcttg catttcattt tcataaaaaa aacgtggcag tctcaaattt
    15121 ttctttttga gatgaacgta agaaaatgga ttttctttta tcacttcttc tttgagaaga
    15181 tactgataaa acgaacgtag gctggctatc ttgcgactga tagaatttct gctatagcta
    15241 ggacgatgat cagctaatgc actcaaatag acacgaaccg taaaatattc cgccttcagc
    15301 agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat ggagaatgtc ttcttcatat
    15361 gcttctttcg ttttttctga atatcctctt tcgacaatca aataacgcaa aaatttttgt
    15421 tcccacgtgg cttctgtcat tttctcacct tccttttgtc ccagagaata atttagcaca
    15481 gcgtttataa aaagacaaat aaattgtcag aatttaacca atatttctaa gtttgttcac
    15541 aattggcgca attcctcaca ttttgtttga gaaattctta agaaaaacgc aagaaaaaga
    15601 gcgagactat ccgtctcacc ctcttcatgg agtttcctta ctcatttggg attgcagttt
    15661 tttctaattc tgtttttatt gcctcattag cttttaaagc tcgttgtgca attgcttcat
    15721 atcttgcttt tttgtctcga atgcgctctg gcagttcagg aaacagacca tagttggcat
    15781 tcatcggttg gaaatgtttt ccttccgcat gagtaatgta gtaagccata cttccaatag
    15841 ctgtttcttg tggaaactcg actggttcca tttcttttgc taagcgtgcg gcgttgattc
    15901 cagcaagtag tccgctcgct gcactttcaa catagccttc tacaccagtc atctgacctg
    15961 caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg tttcagcaat tctggcgaat
    16021 tcataaagct gtttcggtgc atcacaccat agcgaacgaa ttcagcattt tctaacccag
    16081 gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa gtgagtctga aagcccacga
    16141 tgttgtataa agaagctgca gcattgtctt gacgcagctg gataacagca taaggacgtt
    16201 tgccagtttt aggatcttcc aatccaactg gtttcatagg tccgaaaagc atcgttttga
    16261 atcctcgctg tgccattact tcaattggca tacatccttc aaaatatttt tctttctcaa
    16321 aagatttaag ttctgccact tcggcattga ttagtgcctc atggaaagct ttgaattcct
    16381 cttctgtcat cggacagttc aagtaagctg cttctccttt gtcatagcgg gacttcaaat
    16441 atactttatc catatcgatc gtagctttgt ctacgattgg tgcagctgca tcatagaaat
    16501 aaaaaccatc tgaaccattg aatgctttga tgctttctgc taatggatga gaagtcaaag
    16561 gtcctgttgc tactacggtg attccttccg gtatttcagt aatttcttcg tttatcactg
    16621 ttactaatgg atgctctttg acttttcggg taattgtttc agaaaaagaa tcgcggtcaa
    16681 cagctaaggc accaccagct ggcactgctg tttcatctgc cgaagtgatt actaccgaat
    16741 tcaatcggcg catttcttct ttcaataacc ctactgcatt agccaaactg tttcctctta
    16801 atgaattgga acatactagt tcagcaaaat tacttgtgtg atgcgcctca gtcgatttga
    16861 ctggacgcat ttcatataaa cggacaggga ctcctgcttg tgcgatctgc caagcagctt
    16921 cacttccggc aagccctgct ccaacaacat ttaccatttt caatgaaaga acctcttttc
    16981 tattattttt ggatgtcttc ttcgtaatct ccatttacac agactacttg tttaccgcct
    17041 ttgactttct tctcgaccag atatccgtca cattttggac aatttctgcc cactggttta
    17101 tcccaagaag tgaattcaca ttctgggaaa cgggaacaac catagaatag tctgtttttc
    17161 ttagattttc gttcaatgac ttgtccttca tgacatactg ggcaagtcac accgatttct
    17221 tttacgattg gtttcgtatt ccgacaatca ggaaaattac tgcaagcata aaacttccca
    17281 tatttcccta attttatcac catcggatgt ccacagacat cacaatcgaa tcctgctggt
    17341 tcatctttga tttgaatttt ttcgattttt tcttccgcat tcgtcagttc tttttcaaac
    17401 ggtttataga aacgatcaac cactttgacc cacgcttccg tgccttcttc aattttatcc
    17461 aaatctcctt ccatttctgc tgtgaaatgg atatcgacga tttgagggaa gaattcacag
    17521 attaatgaat tgacaatttc tcctagttca gtcggctcaa atcttttttg tgtcaatttt
    17581 acatagtagc gtctttggat cgtatcaagt gttggtgcat aagtggaagg tcgccctacc
    17641 ccgttttctt ctagtgcacg aatcaacgta gcctcactaa aacgtgctgg tggttgagta
    17701 aaatgttgtt ttggttcaat atcaacagat tggactacat cgccttcttc aagttctgga
    17761 agaatatttt ctttctcttc tttgccgtca tctctacctt ccacatacac ttgcatgaat
    17821 cctttgaatt tgatttttga accgttcgca atgaagatga caccattttg ttctaatgtc
    17881 actttcattg tatctaaaat agctggtgtc atctgactag ccacaaaacg tgaccagatc
    17941 aatgtataaa gtttcaattg atctttatct aaatattgtt tgagttcatc tggtgtacga
    18001 agcacactcg ttggacgcac agcttcatgg gcatcttgtg cgccttgagc atttttgatc
    18061 tttcggtgat catgtctaga aaattctttt ccatacgtat cttcaataaa tgctgcggcc
    18121 tctgcttttg ctgtatcggc aatccgggta gaatcagtac gcatgtatgt aatcaagccg
    18181 actgttcctt gtttacccaa agtgattcct tcatataatt gctgtgctac catcatggtt
    18241 tttcttgttc gaaagtttaa cttacgggca gcttcttgtt gcaggctact tgtagtgaaa
    18301 ggattcgcag ggtttctttt gcgctctttc ttttctactt ttttaacttc gaagtctgtt
    18361 ccatcaattc tagcagtgac ttcttttaca ctttgtgcat ctggtaattt ctttttctta
    18421 ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag cttttttaaa attaccgtcg
    18481 atgctccaat attcttctgg aacaaattct cggatctctt tttcacggtc aataataatt
    18541 tttaaagcga cagattggac acgtcctgca cttaaccctt tcttgacctt acgccagaga
    18601 ataggactga tcgaataacc aacgattctg tctaaggtac gacgagcttg ctgtgcatct
    18661 actaaatcta catcgatcgt tcttggttcc ttaaaagctg ctttgactgc gtcttttgtg
    18721 atttcattga agaccacccg atttttttct ttcagatcca acccaagtag gtacgctaaa
    18781 tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg ccaagtaaac tttttgagct
    18841 ttttttgctg cggctttcag gcttttgatg acatcgcctt ttccgcgtat agaaatataa
    18901 tggggttcat aattgttttc aaaatcgata cccattttac ttttcggtaa atctcttata
    18961 tggccaacac tggccattac tttatagttt cgtccaagat atttctcaat tgtcttggct
    19021 tttgcaggtg attctacaat cactaaatat ttatacgcca ttactgaatg gactcctttc
    19081 cattgtcgtg ttcctactat taaaagagga ttcatacaaa tcgtagctaa tcatatccat
    19141 tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg tgttattctt taaattctgc
    19201 caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct tggatcaaat gatgacatcc
    19261 ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa actgttcttc caagatccaa
    19321 tgcttgctgt gctgttatca gagagccgct ttttttcttg gcttcaaaga cacatgttcc
    19381 tttagacaaa gctgcgataa tccgatttct cattggaaaa caatgctttt tcgggccgga
    19441 ttgaggagga tattcactca aaagaagatg atcatttgca atcacctctt gcaagtcacg
    19501 attttcagca ggatagactt gctctatccc tgttccaatg actgcgattg tccgtccacc
    19561 atgagagatt gctgcttggt gaccgtatgt atctatccct ttcgcgcagc cactgacgat
    19621 cacatatcca gcttctatca ctggttttag taaataataa actgtttttt tggctaaagc
    19681 agaagtgtgt cttccaccaa caacgcttaa catgttacaa gaaagtaaag atagatcacc
    19741 attataaaac agtacaattg gcggataggt caagtgaagc agctgagggg gatatttggg
    19801 atcaaagcaa gtgataaacg actgtttcga ttggattttg ccccagttag cgtgaatttc
    19861 ctgccaactt ttgcgaaaca gctcccggtg tttggttatt ttacctatct taatgatgtc
    19921 ttcaaatccg atttctttat aaggatactg catcatccag ttagcaagtg cccattttcc
    19981 ttgactactg attccttttg tataagcaag tttcaataaa ttttcttcta tttgatacat
    20041 cataaaaccg cctttcgtct ctctttctaa taagatacgt aaaagcggct gttttttatt
    20101 gtaaatattt ttttacaggt gcaaaacttt tacgatggat tggcgtaatt ccttgtcgtt
    20161 caagtccttc taagtgttcc tttgttccat aacctgcatt atgcgagaaa ccatatccag
    20221 gataaagcac gtcatattct ttcatcaatt ggtctcgata tactttagca atgatacttg
    20281 cagcagcaat cgacacagat cgtgcatccc ctttgattat tttttcctgc tgaataggca
    20341 aatccaaggt catcgcatcg atcaacaaat agcttggtac aatggacaat ttttctactg
    20401 cttggatcat cgctaattta cttgcttcat aaatattcac atcatcgatt gtctgctgat
    20461 caacgatccc gatcccgatt gccagggctt tttcttgaac ttgttgataa agaagttctc
    20521 gctttttctc tgataattgt ttggagtcat tcagccctaa tatgatctca tttttcggca
    20581 agataactgc agctgccacg acaggtccag ctaatggtcc tcttcctact tcatcaatgc
    20641 cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc aaagcaaaac atttcttcat
    20701 atttttcctt aattaatgct tcctttttca atcgtcgttc aaaagaagcc agtgcttgct
    20761 gcacgccttt gcgtggatca tttcttaatg attctagtgt aggatcatct attctctgta
    20821 tgcttttcaa tcgttctttg atactttgga tggattcagt catcactttt ttgctccatt
    20881 tcttcgcaac gatccagtgt gtatcgtcca agttttcctt gacgaatatc atgcacgact
    20941 aattcgctgc cacgatcata gtcttcttta tacccacgtt ttgctgtaat atccatcagt
    21001 aattccggtg ccaatttttc ttcttgctcg attgataaac cataacgatc gctgattctt
    21061 cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg ctaaatcatc taaatggaca
    21121 agctggtctt tgatcgcgcc tgtcagcgct agttttttgc cgatttcttc atcttcaaat
    21181 ttgggccata aaatccctgg tgtgtctaac aattccaact cattacctaa acgaagccac
    21241 tgctgacctt ttgttacgcc aggcttattt cctgtctgtg caattttctt gcctactagt
    21301 cgattcatca acgtagattt cccaacattt ggaataccta agaccattgc ccgaatagcg
    21361 cgaggcttga tcccccgagc agcatctctt gcccgttttt ctttcaatgc ttctttagcc
    21421 gcaggtacta gacctttaat tcctttactt tcttgtgccg tgatagctaa tgcaagatgg
    21481 ccttgtttac gaaaatattc ttgccacagc tttgtctgtt gtggatctgc cagatccgct
    21541 ttattcaata aaacaaggcg aggtttttgc tgaatgatct gatctagcat tggattacga
    21601 gaagataaag gtaatcgtgc gtctaccaat tcaaatacga tgtccacata tttcattttt
    21661 tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa accattggat ggtcattgtt
    21721 ctctccattc ctttcatacc ggaatattcc ccggctttac ttgttttttt ctttttcaat
    21781 ataaattcac ttttttatta tcttaattgt caaattaagt tagacaaatc atcaatactt
    21841 acgtaactca aaaatacttc caaaggtgtt cgataattca gtgattttct aggaatatta
    21901 tttcgtttcg atgcgacaga ttgaataaaa gattcatcta ctgaattgaa atccatagat
    21961 ttcaataaac catctctacg taacaatccg ttagaattct cgtttaggcc tctttgagac
    22021 ggcgttcctg gatcagcgaa ataaatggca atatcattgg cattactgat ctgtttccaa
    22081 tttgaaaatt cctttccaca atcaaaagtg atggatttga atagattttt cggtacagat
    22141 tcaaaccaat gatttaattt gttttcaata tcaatcgctt gtctaccaca cggtttcaat
    22201 gtgatgataa cttttgataa tcgctcaact aaggtaatta cagcactttt atgtttcaaa
    22261 cctacgatag tgtcaccttc aaggtgacca aactcatttg agaattggct ataatctttt
    22321 tcacgttcat gaatagagcg acggaaagct tgttttccac gtctttcttg atgtccattt
    22381 ggtttacgct tgcctttcat cggtaagtca gaaggattaa atagcccctt tttgaacata
    22441 cgataaatgg tacgagcaga acaacgaata ggaaacgctg cacgaccaac aatcacatcg
    22501 ggtgtccatc cttgaacaac ctttctttga atatattctg attgttcctc aggtaaaaca
    22561 agcgggcgtc taccacagtt tgatttgttt ttcttatact gttgatagta ctctaaggct
    22621 gattttcctt gcttgaagaa caggtatact ttatggatcg tttgtcttga acgattcaag
    22681 aaatgcgcag ttttcgcaac agattgattt attttgaaat aagactctat cattacaagt
    22741 tcatccgttg taagatgggt ataggtcatt tgtactcact ccttataatt ctttggacgg
    22801 aactattttg agtgtagcac aaatgacttt tttagttgtc tagcttaatt ttacaatcgg
    22861 cgttataaag aaattccatc tattagttaa gtgaaagtga accagtcctc attctatcac
    22921 atcttcactt ttttgatgat gcaaattttt attttggttt ctatatatga atctaaattt
    22981 ttgacagcgt ttcctaaata agagatgata gacgaacaaa acagaaagga gacgaaacaa
    23041 atgaatagta tggtttttgt taattttcct gttgctgatg tcaatcgttc cgttgctttt
    23101 tatgaaaagc tgggttttaa aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg
    23161 gtatgggatg acactttttg gataatgctt ctctctcatg atttttataa aaaatttata
    23221 aaagataaag aaatagctga taataaaaaa acaagtggtg ctttgatttc gtttagtgtt
    23281 gaaaatgcgg atgaagtaaa aaaatttgct caaattgcaa aagaaaatgg tggtactttc
    23341 cacgaagtag atatgggaat gcctgaagaa gaaatgtttt cattagaagt ccaagatcta
    23401 gacggaaata cattagaacc cgtatggatg aagatgtaag cagataacta atctgacgtt
    23461 ttttataaca aaaaaacaaa ggcggatgat acgataagta ttctccgcct ctattttttt
    23521 tattcgtaca atctcttttt tacagtttat aaaagcagtc ttctcaccct ttttttaatt
    23581 gttagatgcc agtgtatgta aatcaccaat gtgtcatgac acattggtga ttttatggta
    23641 tccttttatt attaactgac aaaggagaga atattttgaa cactttgatt gcaaaagacg
    23701 tttttagatt gcccgaaatg aatgcaagat tgttgtcgac ctctgctctt ttattagccc
    23761 ttgatgtcat cttatacaag atagcgattg gtcctgctta ttttcaaatc acattcggat
    23821 ttttaagtat ggcgcttatg ggttatctat atggaccgat ctgggctggt ttgcttgaaa
    23881 tgttgtcgaa cactttaaat ttcacgatat ttggttcagg aacgtttcaa gctgcttttc
    23941 ttatcacggc tttccttggt ggatttatta atggcttatt tttatatcat aaaaagataa
    24001 acctgccttc cgtcatcgct gcccaattat tgattatgat cccagtcagc ctttttatca
    24061 attcttggct gatgtctgtt ttgtttggtg cagatttcaa agtaattttt ctaggtagat
    24121 tcatcaggaa tatgatccaa atccctattc aaataatggt tgtttattca tttatcaaaa
    24181 ctttagaggc tagaaaagtg tttaaacgag ctgtttgatt ttacgtacta aggtagtaaa
    24241 ctgattttgg attaaacaat aaagcgtgca gtctcaacag actgcacgct ttattgtttc
    24301 tcactggatc cattttattt catcaagtgg ataatagaca aatcttgctt tacctagaat
    24361 atcagtttta ttgatcgtgc caaacgaacg actatctttt gaaactcttc gattatctcc
    24421 aagtacaaaa taattatctt ccggaagctt ggattgtccc gttagctctt gtagtgtaaa
    24481 gtccgtcgta taggatgcat ttgcatgatc tgatttcagg tttttagtta gataaggttc
    24541 cttaactact ttcccattga tcttcaattg atcattttgg tagctgacat tttctcctgg
    24601 aagaccgatt actcgtttga tataaatcgt gccatctgct aattgaaaaa caacaatatc
    24661 aaatcgtctg atctccgaaa atttttccat cacaaccata tcaccttgct tcaacacgtt
    24721 ttccattgaa ttgccttcca ctggtaccgg gatgaataaa aatccccgca acatgaacgc
    24781 aaggaataca gaaacaagta agtatttgaa tacaagccag aagcgattaa tatagcgttg
    24841 tttttttgtc aatctctatc aatccatccc tctttttttc attctttctt tattataaga
    24901 aaaatatcaa aaaaatgcga ctagatagct gaaataaaaa taattctttc aattaggcta
    24961 ttggacaaaa aacttttttg atcataaagt acaaaactat ctcttatatg tcatgattat
    25021 tttctaaata tttttcccat gcttcgtcaa agatatccat ggacggcaaa tagtcgacat
    25081 tcagttccag ataattagaa atttcttcgt agctagagct gtgcttagga aattggatgt
    25141 ctttaccggc ttctgtagcg aaaacagata aagcatcatg tgtgccaccg cctcgcagtg
    25201 tcattaaata atgaaaaaag cttcgattca cccttcgttc ctccaatttt cttcgataaa
    25261 ggcagcccgg cgtgcatggt ttcttttaaa attttcagga tgctttttat aatactcttg
    25321 atggtcttct tctgctagat aaaatggttg ggctggttcg atttttgtaa cgattggctg
    25381 ttcgaaacgt ccactttcag ctaactgttt cttacttttt tcagcaatga tccgctgttc
    25441 ttctgtcaaa taaaagatca ccgggcgata attgtcaccg cgatcctcga attgtccgaa
    25501 agcgtcagtt ggatcagttt gctgccagta aagttctact aattgttcat atgaaatcaa
    25561 tgttggatca tagataatct ccactgcttc tgtatgtcca gtcgtttggc tttttacttg
    25621 ttcataagtc ggatctggta catgccctcc agtatatcct gacatcactg tatggatacc
    25681 aggaagcgtg tcgaaaggct ggatcataca ccagaagcag cctcctgcaa atattgctcg
    25741 ttccattatg attccccctc taatactact catccgatga ttttttattt tcttttgtct
    25801 tgctttcagt actgctggtg ctagttttgt tggtatttga gttgtttgaa gcatcagagg
    25861 tatcttttgg caaatataga ctaaaccgaa tgtcatcatt tactaagtcg attttatctg
    25921 ctttaatata catcccgttt tgcattcgga attgatccaa cctaagcatg atagtcagat
    25981 catctgtatt gacttcgatc caatctggta atttatagtt tctcttgatc atattcatca
    26041 catctttgat tggcaaactc aatgttccga tagaaagact tctagctttt agttggacat
    26101 ttccgttttc catcacatac ggatcaaaat ataaatagaa atcgatagga aaacctagta
    26161 cttcgaaagt accattgagt agtgcttcat tttccaaata aaatttatat tcaatatcac
    26221 tatctttttg atactcacta aggaaaaagc tgatgatttg attgactttt tctttatttg
    26281 aattgatcgt taagacagga tctccttgtc tttccgtaag agctggtatt tctgggagtt
    26341 ctggttcccg attagcaaaa atacggctgc ctaaaaatac ggtcgaacca agtactaccc
    26401 caagaaggat aaggaaggcg aatttccagt aatttgtttt ctttgttttg tttgaagata
    26461 cgtcagatct tgttttcatg tttattgatc atcctccttc tctaaccatt ggttttgtgt
    26521 ctcgatcagc tgatctctca ctgcatttgc catcaattga taacctaaat tatttggatg
    26581 gaaatgatct tcttcataga gcacattatt tttgatgcca tttagatttt ctgtcgtgct
    26641 agttgtttga tctgttcctt cttctgtgac acctacttcc tgattcaatc cttgataaag
    26701 caaatcattg attgggataa agtagcagtt cggcattttt tttgtcaccg tttctgttcc
    26761 tttgttccaa ttgttaacaa ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa
    26821 tggattgtag atgcctaaaa tgtaaatagg tgcggtttta ttgagggatc gaaattcttc
    26881 caatagttct gtgacatttt cctgatagtt tttcagtggc tttttaaagg aattgaccgt
    26941 taacccgaat aaattatttt ggataacttt catcaaatca ttaccgccaa cagtcagtgt
    27001 gataaaatca gctgttttga tattgtttcg aatttcttct tttgttttta ctcgctttaa
    27061 gatctggtca cttcgttcac catttacacc atagttttct gtttcaaccg cggtcaaatt
    27121 ataacggtct tttagatcat ttgcgacaag gggaacgaat cctccttgct tggtttgatc
    27181 tcctactcct tcagtcagcg aatctccgat agctatgtag tgaagttttt cttttttatt
    27241 tggatcttct ctcacagtag ttactggttt cattagtggg ccagctttcg gtatagaagc
    27301 agctaaacct gcatagatga tgatcgcaat taataatggg aacaaaacga ggatcaaatg
    27361 tcgtttcgtt aatttcattt tttcacctct tgtattcgat tggttctaat gttatcaaaa
    27421 attttatcaa aaaggtagtc tttatgaaaa aagaaaacaa ttattttcct aattttataa
    27481 agaaaaagag aataagctgc aagcctttca ggctgagggc ttatcctctt ttcgactaat
    27541 ctacataata catgattgca aacgcgtctt taccagtatg cgtagcgata accggattcg
    27601 tatgcagtac agggatatgc atgtccggga ataattcttg caaaccctct ttgaattctt
    27661 gcgccaattc ttttgcatcc gcatgtgaaa taccgatttg tcgcacattt ggcaattctt
    27721 tcagttcatt tttcaaatta ttaaaccatt tatcgaacgt tttctttcct cgccccttag
    27781 ttacagggat taattcagaa tgggcgaaat tcatcaccac gcgtatgttc aaaacactag
    27841 acagcaaacc agttgttcgg ctgattcttc caccttttac caagttatct agtgtggata
    27901 ttccgatgta taattttgta ttgtcttgta cttttttgac ttcttcaagg attttctcta
    27961 ctgttttacc ttcttttgct aattcagctg ctcgaattac ctgaaaagat aatccttggt
    28021 cagtggtatc actgtcgata accgttactt tcgtttttgt tagattactg gcttgtcttg
    28081 ctgcttctac tgttccactc aagccttttg tcatatggat cgaaatcact tcactgccgt
    28141 cttctcctag ctcatcatac atcgagatga attctccaat tggtggttgg cttgttttgg
    28201 gcagattttt tgctttcgcc atcatttcca taaatctttc gccgtctagt tggtcatcat
    28261 cgggatatac tgttccgtcg atcatgatgg atagcggcat gacacgaatg cctaaagcat
    28321 cgcgtacatt tttttccatc gtacatgaag aatctgttac gatttttaca tttgtcatta
    28381 ttttctctct ctctttcttt aacaagatgc cttattcatg ttaaactaca tgtaagacaa
    28441 tttctatttt tagtataaca gacaaataat gatttttcat taattacacc tagtaaagga
    28501 gccgttttca tggaaaaaac gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt
    28561 aatgcagtca cccatggaat tgggactgga ttgagtatcg cgggactagt ggttctttta
    28621 gtaaaaggtg cccacttgca ttcctccctc catattgtct cttatgctat ctatggatcg
    28681 atgatgattc ttttattttt aacgtctact ttgtttcatt cattgatttt tacaaaagca
    28741 aaaaaggtgt tccaagtatt cgatcatagt tcgatttttc ttttgattgc aggaagttat
    28801 actccttttt gtttgctgag tatccgagga tggctaggtt ggactttatt tgtattaatc
    28861 tggttgtttg cgattcttgg cattatatat aaagcgttga cgttgcataa acgggagaga
    28921 gtatcaaaag tatctacgat catctatatt tttatggggt ggctctgtgt cgtggctgct
    28981 gttcctcttt atcactctct tggaatgact ggtgttattt tgatggtagc aggcgggctt
    29041 tcttacacga taggcgcatt cttttatagc ttgaattcta tccgctatat gcatgttgtt
    29101 tggcatttgt ttgttatgtt aggtgcaggt tttatgtact tctctgtttt attctttact
    29161 taataaaaaa aataaagcta tggatgggac agctctaaat aacagctagt ctatccatag
    29221 cttttttata tcgtcaattc gcctgtcccg ctttttacag gattctcatt acgctgatac
    29281 gtttcaaatt gatgcggata acgatttttt tcatccattt ctccttgact agtattgatc
    29341 agtgaccaat cagaccagtc aacaggtgga aaataagcat ctccatcgaa tacatgatgg
    29401 atgactgtac ggtataatat cgtacaaaat ggcagaaatt cttgataaac agctgaccct
    29461 ccagcgatga aaaagatacc gtctgtttct ttttccagag caagcacgtc ttccacacta
    29521 tgcatgacat gcacgccttc tgctttatag gaagaatctc tcgtcaaaac aatcgtctga
    29581 cgattcggca acggacggct acccatccct tcgaatgttt ttcgtcccat cactagtgta
    29641 tttgcttctg tcatttgttt gaagaatttc agatcatttg gcaaacgcca aggaagacgg
    29701 tcttcttttc caatcaatcc gttttcatcc tgtgcccaaa ttgcagctaa cattttctca
    29761 tcctttctga ttctaaacag cgataggagc tttgatcgcc ggatgtgggt catatccttc
    29821 taattggata tcttccatct caaaatcaaa tactgactgc ttttcctgat tcaaaactaa
    29881 agtcggaaat gcgcgtattt ctcggctcaa ttgttctttc atttgttcga tatgattgat
    29941 ataaagatga gcatctccta atgtgtgaac aaattcgcca accgctaaac ctgtttcatg
    30001 tgcaattagg tgagtcagca acgcataact tgcaatgttg aacggaacgc ctagaaacac
    30061 atccccactg cgttgataca attggcagct tagccgtccc tcttgaacat aaaattgaaa
    30121 catcgtatga caaggaggca aagccatgct aggaacatct tccggattcc atgcagaaac
    30181 gatcaatctt ctagaatcag gagtcttttt gattgcttcg atcacttgtt tgatttgatc
    30241 gataaagcca ccgtcttttg tttcccagtg acgccattga tagccgtaga tgtgtccaag
    30301 atcgccgtat gtttcagcaa aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct
    30361 ctgcttttta tattcttcat tgaattcagg atctgtcaaa catctatggc caaaatcagt
    30421 catatctggt ccattatagt ctggactttt gatgtagcgt tcaaatgccc attcatccca
    30481 aatatggttt ttattttgta atagatagcg gatattcgta tctcctttta aaaaccaaag
    30541 caattcgctt ttgatcaatc caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt
    30601 ttgcagatca aaccgcattt ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc
    30661 ttctttacga tgcccttctt ccaacaattt tcttcctaag tcaagatacg cttgttccat
    30721 cctttttcct cctaatcttc tacccattca ctcaagtatt cccaccgtgc cattttttct
    30781 tcaagttgtg cttctgtttc agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta
    30841 tctccttgat gattcatctc ttcttggagc aatgagattt tttcttcaag ttctgcaatc
    30901 tcatcttcaa tcgtttccca ttctttttgt tccatgtaag ataatttctt cttttcagca
    30961 ggttccttgc tctgtatttt tggtttcgct ttttcagcag gctgctgggt ctctttttgc
    31021 ttcagtaaat aactggacat gtttccgtaa taaagttctt gttttccgtt tccttggaat
    31081 actaataact tatctgctac cttgtccaag aaataacggt catgggaaac agtgatcaca
    31141 gcacctggaa aacttctaaa ataatcttcc aaaatcgtca atgttgctat gtccaaatca
    31201 ttggtcggtt catccaataa taaaacatta ggttgctgaa tcagcagttt caacaaaaat
    31261 aaccggcgtt tttctccgcc tgaaagtttg cctatcagtg tcccatgcat aaatcttgga
    31321 aataaaaatc gttcaagtaa ttctgctact ccgatttgac tgccatctgc tgtttttgct
    31381 tcttcagcag cttcttgtaa gtaagcaatc atccgtttat ctggtgccat ttcttcattt
    31441 tcttgtgtat aatacgcaag atgaaccgtt tcaccgatag acattgtacc gctatccagg
    31501 ggaattctgc cagctagaat attaagcaat gtcgattttc ccgcaccatt tttaccagta
    31561 atacctagtc tttcccgaga ttgaatcaat aaatctaaat gttctagcag tgtttggtca
    31621 tttatacggt aactgccatc tttgatttcc agtacttttt tccctaaacg ttgtgtcgct
    31681 aggttcagct ctaaatcatc ttcttggttt acttggtaca agttctcctt tagatcttcg
    31741 aaacggttga tccgtgcttg ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa
    31801 gctaattcct gtttataaag ccgcttgcgc ttttcttctt gttctacttc tacacgatca
    31861 cgttctgctt tttctaagac gtaagcttca taatttcctt tatactcgta taattttcca
    31921 aatgaaagtt cgaagattcg gtttgctact ctatctagaa aataacgatc gtgggtaacc
    31981 atcaatagtg cgccacggta ttgtttcaaa tagttttcca accactcgat cgcttcataa
    32041 tctaaatggt ttgttggttc gtctagtaat agtaaatcgg gttcatcaat caacacttgc
    32101 gccagactaa ctcgtttttt ctgaccacct gataaagtac tgattttttt atctaattca
    32161 ctgattccta atttttgcaa aataatcttt gcgttggtat ctgtagtcca agcatcttct
    32221 ttattcattt gttcttctgc tttagcataa cgtttttgca catcagggtc ttctccgttt
    32281 tcccctagtt cgatcaatgc ttcctcatat gctcgaacag tttggatcaa ggggctgttt
    32341 ccttgaaaga ctgcttggag aacagttaat tcatcggaaa attcagctgc ttgagaaaga
    32401 taaccaatac ggtaatcagt tggataaaac actgtttgcc gatcaccgtc gctgctatcg
    32461 ataccggcaa taatatttaa gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg
    32521 atacggtctt tttcgtggac caaaaaagaa atctggtcaa acaaatcttt ttcaccataa
    32581 gtttttttga ggtcagttac ttttaattct ttcaacatgt tccctcctta tcttggatat
    32641 tgtatcataa aaatagtgaa cttcaggaaa atttttttgg tatttgttct ttttgtgttc
    32701 tcaa
//
LOCUS       DAYYAJ010000018        31036 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000018 DAYYAJ010000000
VERSION     DAYYAJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.018"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1854)
                     /locus_tag="ACHATZ_000724"
     CDS             complement(88..1854)
                     /locus_tag="ACHATZ_000724"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403277.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1814..3568)
                     /locus_tag="ACHATZ_000725"
     CDS             complement(1814..3568)
                     /locus_tag="ACHATZ_000725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403278.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3678..4247)
                     /locus_tag="ACHATZ_000726"
     CDS             complement(3678..4247)
                     /locus_tag="ACHATZ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8403279.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4265..5680)
                     /locus_tag="ACHATZ_000727"
     CDS             complement(4265..5680)
                     /locus_tag="ACHATZ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403280.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5691..6359)
                     /locus_tag="ACHATZ_000728"
     CDS             complement(5691..6359)
                     /locus_tag="ACHATZ_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8403281.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6489..7073)
                     /locus_tag="ACHATZ_000729"
     CDS             complement(6489..7073)
                     /locus_tag="ACHATZ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8403282.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7404..7637)
                     /locus_tag="ACHATZ_000730"
     CDS             complement(7404..7637)
                     /locus_tag="ACHATZ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8403283.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7652..8602)
                     /locus_tag="ACHATZ_000731"
     CDS             complement(7652..8602)
                     /locus_tag="ACHATZ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8403284.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8599..9441)
                     /locus_tag="ACHATZ_000732"
     CDS             complement(8599..9441)
                     /locus_tag="ACHATZ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8403285.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9443..10162)
                     /locus_tag="ACHATZ_000733"
     CDS             complement(9443..10162)
                     /locus_tag="ACHATZ_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8403286.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10710..11012
                     /locus_tag="ACHATZ_000734"
     CDS             10710..11012
                     /locus_tag="ACHATZ_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403287.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            11084..12310
                     /locus_tag="ACHATZ_000735"
     CDS             11084..12310
                     /locus_tag="ACHATZ_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8403288.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12398..12790)
                     /gene="rpsI"
                     /locus_tag="ACHATZ_000736"
     CDS             complement(12398..12790)
                     /gene="rpsI"
                     /locus_tag="ACHATZ_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8403289.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12806..13234)
                     /gene="rplM"
                     /locus_tag="ACHATZ_000737"
     CDS             complement(12806..13234)
                     /gene="rplM"
                     /locus_tag="ACHATZ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8403290.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13768..14235)
                     /locus_tag="ACHATZ_000738"
     CDS             complement(13768..14235)
                     /locus_tag="ACHATZ_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8403291.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14355..15893)
                     /locus_tag="ACHATZ_000739"
     CDS             complement(14355..15893)
                     /locus_tag="ACHATZ_000739"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8403292.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15973..16659
                     /locus_tag="ACHATZ_000740"
     CDS             15973..16659
                     /locus_tag="ACHATZ_000740"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8403293.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16860..17387)
                     /gene="msrA"
                     /locus_tag="ACHATZ_000741"
     CDS             complement(16860..17387)
                     /gene="msrA"
                     /locus_tag="ACHATZ_000741"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8403294.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17471..18148)
                     /gene="pgmB"
                     /locus_tag="ACHATZ_000742"
     CDS             complement(17471..18148)
                     /gene="pgmB"
                     /locus_tag="ACHATZ_000742"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8403295.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18145..19275)
                     /locus_tag="ACHATZ_000743"
     CDS             complement(18145..19275)
                     /locus_tag="ACHATZ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8403296.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19275..21458)
                     /locus_tag="ACHATZ_000744"
     CDS             complement(19275..21458)
                     /locus_tag="ACHATZ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8403297.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21455..22480)
                     /locus_tag="ACHATZ_000745"
     CDS             complement(21455..22480)
                     /locus_tag="ACHATZ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8403298.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22525..23352)
                     /locus_tag="ACHATZ_000746"
     CDS             complement(22525..23352)
                     /locus_tag="ACHATZ_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8403299.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23349..24416)
                     /locus_tag="ACHATZ_000747"
     CDS             complement(23349..24416)
                     /locus_tag="ACHATZ_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8403300.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24442..25278)
                     /locus_tag="ACHATZ_000748"
     CDS             complement(24442..25278)
                     /locus_tag="ACHATZ_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8403301.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25275..26144)
                     /locus_tag="ACHATZ_000749"
     CDS             complement(25275..26144)
                     /locus_tag="ACHATZ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8403302.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26214..27497)
                     /locus_tag="ACHATZ_000750"
     CDS             complement(26214..27497)
                     /locus_tag="ACHATZ_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8403303.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27511..29196)
                     /locus_tag="ACHATZ_000751"
     CDS             complement(27511..29196)
                     /locus_tag="ACHATZ_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8403304.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29471..30496
                     /locus_tag="ACHATZ_000752"
     CDS             29471..30496
                     /locus_tag="ACHATZ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8403305.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9472 a         6205 c         5057 g        10302 t
ORIGIN      
        1 ttagatgctg tttaataaaa ttattctttt tgaaaaagaa ttataactat atcccaaaaa
       61 ttgtaggtta atttaaagcc tatttcatca tcgtgattga gcttcttttt catagacgaa
      121 tttctcagat agtttagttt tctttatgag atttttgtat atttctgacc ttcgatacaa
      181 ctcattatga gtaccttgat caactagttt tccttgatcc atcactacaa ttttatttac
      241 cttttcaata gacttcaagc gatgcgatat aattagtact gtcttatctt gtattaaacg
      301 atttaaactt tcctgaatac gtttttcatt ttccgcatct aaactggcag taatttcatc
      361 taaaattaaa attggcgcat ttttcaaaaa agcccgtgct atggataggc gttgtcgctc
      421 tcctccagaa agtgaacttc cgttttctcc gaccaatgta tgataaccgt caggtagttt
      481 ttcaataaag tcttcgcagt tagctaaacg agctgctttt ttcacttcct catctgacgc
      541 ttgactattt ccaatacgta tattttccaa aatacttgta ttaaacaaag ttacctcttg
      601 aaaaacaata gcgattttgc taaacaaaga agctggagat acacgtttaa tatcataccc
      661 atcaattaaa atacagcctt catcataatc atacagtcta gacactaatt tcaagatact
      721 agttttcccg gaaccactag cacctactaa cgcagtcact tctccttgtt tagccgtaaa
      781 agagatatga tctaaaattg gggtattgtt atcatatgag aaagagacat ctcgcaactc
      841 aatgtcaaaa gatttcagag gactatctga tccctcttgg atactggttt ccttcatagc
      901 tctaattcgc tgtattttag gagctaaata aaatatttca agcactgctt ctttcataga
      961 atcgaaggaa tcctttattt ttattgcggc tagtaagtaa ccaacgacgt agagtatagt
     1021 cacttcaccc gttaacagta agtgaacacc tactagaatg actactgcta aactaacata
     1081 tgagaaaata gaggataagg ccatgactga aaaagtactt aattctacct ttaaatgaat
     1141 cctttcactc tcttccattt ttttgaaaag tctgtcttga acttttttag atagattaaa
     1201 gctattaatc tcttgctgca attctatagt ttcttgaaat tcttccgagt tctctctcaa
     1261 agtatcgtaa tatttagtat tggctttcgt ttgggatttc tttgataaca agattaagac
     1321 aaaactaaat aacgttggca aaataacagc taatcccatt ttgacattac caaccaaaag
     1381 catcactgaa ataaaaggga aaaacagagc cataccgccg gctttaggta ttgcatgact
     1441 catcgcatgc tcaatacctt caacgtcaga catgatagtt tgagataaat ctgataaatt
     1501 atgttttgaa aaataggata atggtaaatt tgacaaatcg tctgcaattt gcactcttaa
     1561 atgcgcactt tctttataag tactattata taagttttca tactccctat ctaacaacca
     1621 atacatgaaa agcaaagtaa caaacgatac ggcaaaataa agccaacggg gctttgtatt
     1681 ttctaaaact aattcgtcta tcagcatcat tataatgaat gctggtacca tattaatgca
     1741 gtatacaaaa aaactagaaa tagaagcttt agttaaagct tctgcgccct gatcacttaa
     1801 agcaaatttg tttttataaa aacttttcat tgtcaagcct ccaatcattt gcattctcat
     1861 aattagtgac caattgttta tacacttgat gtttagccat taattcatta tgatttccac
     1921 gttctactat tttcccttct gacaagacaa gaatttcatc tacagattta atgctggata
     1981 atcgatgagc tatcataata actgtttttt ctttcatcag atgttgaaaa gccttttgta
     2041 gttcatactc attatctgga tcaatagatg cactggcttc atccataata acaatctttg
     2101 catcttttaa aattgctctg gcaatagcta tacgttgttt ttctcctcct gacagataaa
     2161 ctcctttact accaattaag gtatgctctc tttcgggaaa tttttctaaa atcgattcgc
     2221 aaccggctaa tcttagcgcg tcaaatactt tacttgctgg cgcttgagga tttgctatag
     2281 aaacgttatc gtaaatactc tgattgaata gtttcgtatc ttgaaagaca aaagcaattg
     2341 tgttaataat tgtgtctttt gaatagtgat ttaaaggctt accaccaatt ttaatctgtc
     2401 ccttatccac attgtagaaa cctgaaatta gtttagctat cgtactcttt ccgcttcctg
     2461 attttcctac taatgcataa attctttttt gttctaaaac taagttgaaa ttttctaaaa
     2521 cacactcttc tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa tttttaatct
     2581 gtgtctcgtt cccatatttt agttggtctt gcttcatttc taaataaagg gagtctagtg
     2641 tttcgactgc atatcttgct tcaaacaagt gcatacttac atacattatt ctcatgaaag
     2701 aaacaaaaat aattccactt aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg
     2761 gagtggtaag agaagttata aaaaaggtta taggaactat caaaatgcaa actatgccaa
     2821 aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact caaagaatac ttatatgcaa
     2881 acttagcata atcgttaatt gctttattca aggctttaaa tgatgttact ttagcaccaa
     2941 atatctttat aacttgaatt ccgcggatat attctaccgt ttcagagctt aacacatcta
     3001 acgattcttg atatttttga ataaaatttg tattctctgt cattttcttt aacaagaata
     3061 caccaattac taacatggcg attagaacca atccaatttt aaagttaatg aaaaagccta
     3121 aggcaactga taaaacaggt gttactatgg cttgagcact atctggaatc aagtgagcaa
     3181 ttgccatatg tgttttagcc gcgttgtcat caattgtctt tcgaatatat cccgaagaat
     3241 ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct aattcccttt tttctcaaat
     3301 tagtctctaa gcgaaaccca aggatatgag aaaataagcc agaaaaaata taaaataaag
     3361 ctcctaaagt taaccaagca acagtttgaa ccgcataact cttagctaaa tattcatctc
     3421 ctacaataat tagagactga agaaatttgt aaatactgta atatccatag actgttaata
     3481 cgacagaaaa atcagaaaat aaagtggcta accatccgta gtatttcatt ttagggacat
     3541 aactaaataa ttttttgtat actttcattt tttcctcccc cgcattcaat ttcttctctt
     3601 attcgtttac aactgttaat gctttccgta atagcgtaaa ttagagatgt acaaacagat
     3661 aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga cttttcttcc aatcaataca
     3721 ccaatcatag ataaacttgc tacaactaac actgaaatga ttccccatat acctaccgca
     3781 tagtggatca ttgcatccat tgttgcagca gattgtcctt tatctatcca atcttgacga
     3841 aaacgatctg taaagaacca tataggaatc cactgtccgg cagcactgcc taattgcatt
     3901 agagcccaag acgcaacttg aggaattgct cgatcatatc caaatacaga tagaataata
     3961 tcagctaaga tcgccatact aatcgaactg ataaaccaag gtaagtacgc cccgcccatt
     4021 aacgtaaaaa ataacaataa aacaattgtg taaatagtaa aaattccttt tctagggtag
     4081 ttatagctaa tataaacaaa tatgattcca gataataatc caaaaatccc gggggatatg
     4141 ctgtgaccaa acgccccacc aatactacta accattattg aaaaaaagta aattactagg
     4201 gaaataactg tagctaaacc aataaaaata taatcttttg ttttcatata tattctcctc
     4261 aattctattt cgttatgtca ttaaaaatct gttctatcaa agttgaatct tctactctaa
     4321 gttttatatc tttttttatt ccaccatttt ttaaataaat aattctattg catgttttat
     4381 aaagaaattc cggatcatga gtagctataa ctactgcacc cttttctttt aagtctttaa
     4441 taaggtcagc aatcctttcc atattaatat aatccaaccc acttgtaggt tcatcaaata
     4501 gaaataaatt agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac
     4561 cggataaatc aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt
     4621 tttcttgctg atctttacta actatttcat tccccagggc taactcgctc ctcactgatt
     4681 ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt
     4741 tattttcgat tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg
     4801 ttgttttccc cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa
     4861 aatttatgtc ttttagaata ttttcataag taatttcttc aacttttaat acatctgcac
     4921 ttaccttttc gatataggga acttcaatcg aactattaaa aatgtcaaat ttccttaatg
     4981 cgttattgtt ttcagttttt aacaactgaa tagttgtagt ttttacagtt ctatttttaa
     5041 caaatagaac ttgatcaatt aattctttca aatagtaaaa acgatgttca gctataacaa
     5101 tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc aagtttctta gtcgctttga
     5161 agtccaaatt tgaagacggt tcatccaata taattaattt agagtttagc gataaactac
     5221 atccaataga aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata
     5281 tatttgaaag atctaataat tcagctaatt cattgattct tttatccata tgctcttttg
     5341 taaatccaaa gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt
     5401 gtcctctagg atcttgactt acgtagccaa tattttttga tatactttct tgtgttaagg
     5461 attgtagcga gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg
     5521 gaataagtcc attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa
     5581 tcaatgtaag actgcctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa
     5641 catccttata tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa
     5701 atacttaaga aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt
     5761 gttttatagt aagaacttct tttcacagga gattctatcc cctttgttat tgcattagcg
     5821 gtcatttctt cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa
     5881 tattgcagag gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc
     5941 attcttattt cagaacgata agtcggaaag aatctgatca ctactgtaat acttaacatt
     6001 atttttttcg gtaaatgtat tttttctaaa gaggataata attcattgct agtatagaca
     6061 ctaaccaaca accatgagat cattacgacc ggcaacattc gaaatacaat atataaggat
     6121 gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat caataatttt cattactaaa
     6181 aacagaactg aataaatcaa gatgaaataa atacatgtct ttattttacg aaacatcaat
     6241 actaaaactg tagcaataat gaaacacccc acttgaaata tatttccctt tcctaaaaaa
     6301 ataatgaccg gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact
     6361 ctttcacctc caaaaaataa gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta
     6421 ttcagataac agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct
     6481 ttttttaatt aactcagtaa ttcaatccac ccaggaacag aaaattgata caatcttctg
     6541 gttaatagca tagtcttttc ttttgaatag ctatgcttca atatatcata gaatgtagcg
     6601 tagaatgagt aaatgtagaa gtggatttct tcttcattaa agactaaatt ttgattgatc
     6661 attccgatag tctttaactt atctagacaa cagactgtct ttttcgtcac aaatgatgta
     6721 attttttcaa caatatcttg atatttggtt ccagctgatt ggaataaaat caaatcgaat
     6781 atacgtttat tctcaaaaat ataattaata aatggatcta attcattgaa gttctctctc
     6841 caacttttct ctatatctat attttctaag tttaagaggt atttggtaaa ctcttcatta
     6901 tacatattaa taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta
     6961 gaagtaaaat gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt
     7021 atcttagttt gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt
     7081 tggtgagtta taatataatc tctggtttct atgggtactt cctcctaata agatgaatta
     7141 actttattat cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat
     7201 tccttagagc taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa
     7261 gaattaatag actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa
     7321 aaaattatat ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt
     7381 atcctcccta aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt
     7441 ttccctttat cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc
     7501 agcatctcac ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt
     7561 aatttctttt ttctttttga taattcctga attgctagta aataaattag tatagtagtc
     7621 gttttaattt gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat
     7681 ccaggttcca tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg
     7741 aatcagtaaa aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg
     7801 gtctatcatc cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt
     7861 caactaagtg ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt
     7921 aagcagaagg cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa
     7981 tcattttttt atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat
     8041 ccaatttttc aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt
     8101 tttccgctaa ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc
     8161 aagcatgagg atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc
     8221 catcacttgc tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg
     8281 taaaccaagc atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt
     8341 ttgaagtttt ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag
     8401 gtacgatact gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa
     8461 tcgaattagt aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac
     8521 tatttattga acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta
     8581 ctgctttcca ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg
     8641 tgagattaga aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc
     8701 tgctatgtta aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt
     8761 tgcagaaata aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg
     8821 tgtaattaac attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac
     8881 taaagttaat agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt
     8941 tgccattaat ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac
     9001 caacactaaa accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat
     9061 atttccaaat aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac
     9121 tcctagtgct aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact
     9181 attgcttttt ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat
     9241 aaaaaaatta atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat
     9301 agcatcaccc ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac
     9361 aatccctata actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc
     9421 attaataaaa ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg
     9481 ctttttttaa attttcttca ttaaatacag attctacact tccgtgagca attagatttc
     9541 gattcaaaag aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa
     9601 ttagcactgt ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac
     9661 taactaagtc tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa
     9721 gacatcttgc taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat
     9781 tagaatattt tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat
     9841 ttttaattct ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta
     9901 cttttattgg aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca
     9961 gttttttatt gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta
    10021 acattgcttt aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag
    10081 caccttcttg tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat
    10141 taagcttttt cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca
    10201 tatggtatac aaaaattata ttttagtcaa ccctaaattt tatagtttag atttttttct
    10261 gttgctgaag attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca
    10321 aaatatataa aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc
    10381 gtatggaaaa attatacttg aagtggatag cgggttgaat aagttgataa gcatcaaaac
    10441 atttataaaa attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac
    10501 tccatttaac ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa
    10561 taggaaaaaa actatttttg atcattgaca attaaataca atttttgaat aacgatccct
    10621 gctattcgag ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat
    10681 ttctttttgt tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg
    10741 taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca
    10801 aaaatttcat aactactatt ttaggagatg attttataga atccagagtc tacctgaata
    10861 aaaaacaaac ctgtgcattt ctaggaatcg ataataatac tctagataag tggattcgta
    10921 tatataattt cccagtaata agagttggtg gtgtagttcg ctttgacgta gaagatatta
    10981 aagactttat gaatacattt aaaataactt gaaattttct tctcgccgca tatactaaaa
    11041 ttagtgaaat atactgcctt acttttttta aggagtgagg attttgccta caaagctttc
    11101 aaatgggaaa tataaaacta atctgagata tccaaaaagg tttaaagaaa taacaggaat
    11161 tgcatcagaa aagtatcaaa aaacatttcc aaataggcaa ttggcgatta aagccgaaaa
    11221 tgatatgaag aagaaaatag aaaaagtttt gagagaggag aatgcaaact cattagaatt
    11281 aaaaggaaaa ataacgttta aaaaatttta cgaatctaaa tggcttccta gatatgaatt
    11341 aggtcaaaca attagatcaa acaggcctcc tagtgatata accattagta atacaaaaga
    11401 tatatttcgg ttgcatattt tacctatgtt tggtgaatac gcaatgaatt atctaaatat
    11461 caatactgag ataatatcag atgaattaac caaaaaatcg aaagaatatg caaatataaa
    11521 aatcataaaa ggatatgttc gttccatgtt cgatattgca gaaatactaa attatatcga
    11581 atttaatcga actactaaaa ttattcaaag tatcactgca cctaaaaaaa atgctttaga
    11641 agaaaaaaga atacaagaag gtaagcaagc actatcatca aaagaactta ctaattggat
    11701 agaagcagta aatgatgatt ttaataatca tttacttact tttcatgatt acacactttt
    11761 tatgattacc ctgtatcttg gtgatagaaa aagtgagact tacgctttgc aatggaaata
    11821 tattgatttt gaaaaacaaa cggtccgtct taaacatact ttagataaat atcaaagaaa
    11881 aaagtttaca aaaggtcgaa aagataccgt cattcaagta ccagaagttg taatgacatt
    11941 gctaagtgaa tggaaatcgg tacaagctga tcaattgttg aaattaaaaa ttaagcaaac
    12001 gttagatcag tatctcttta cttatactaa accatctgga gaagtaaatt gtcctgttca
    12061 tgccgattac ctaaactatc gaatcaattc aattaaaaga aggcatcctg acttagttca
    12121 tttatctcca cacaaactcc gtcatactta tgcaactata gctagacaag gtggagcaga
    12181 tatgaatcaa atttcaaatg ctttaacaca ctcagatatt tctacaacaa agatatatgt
    12241 gaacacacca gatatagttg ataaggctgt ttttgaagct tttcagagag gattgaataa
    12301 gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca aaagaagcac tttccaaata
    12361 attgaaaagt gccagaaacg ttgatatata aaggatatta acgttttgag aattgtgaag
    12421 ctttacgtgc tttcttaaga cctggttttt tacgttcgac catacgtgcg tcacgagtaa
    12481 gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc aatgcacgag
    12541 cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt acgttaacaa
    12601 atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt acttcacgca
    12661 agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt ttgccagttc
    12721 ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg atatattgaa
    12781 cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa acttctggtt
    12841 gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta aattgtttgc
    12901 ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa cgacgagaat
    12961 ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag ttgctgtgac
    13021 ggtaataaat tttgtcagtc gcttttttac ctgttaattt cactttgtct gcattgatga
    13081 cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt ttaccacgta
    13141 gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg tctactacat
    13201 accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg ttattttcct
    13261 ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt tgtggggcaa
    13321 acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt tctgcttgat
    13381 tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg gtcgtttcct
    13441 gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc cttattttga
    13501 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat aaaacataag
    13561 aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct tacaacctct
    13621 tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
    13681 ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
    13741 tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt tgcccaaatg
    13801 agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa agatatcttg
    13861 tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa gatctgctag
    13921 ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat cccagcggcc
    13981 aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat aaggtgaacc
    14041 gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat catccatgaa
    14101 ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg
    14161 gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata cttctttggt
    14221 ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa taatagtaga
    14281 ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt tcgctttttt
    14341 atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg agcaaattgc
    14401 tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat tttctgatat
    14461 tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa ttcctgtgct
    14521 tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc tcctaatgcg
    14581 gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc catcatctga
    14641 cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag ctgattttcc
    14701 tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag aatcgccttc
    14761 atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg
    14821 tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc attggcaccc
    14881 gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc tttttcttta
    14941 gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat gccagcacca
    15001 ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca aaacgtctcc
    15061 atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct cgttcctacg
    15121 gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag cggcccgtct
    15181 gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc tgtttttgat
    15241 ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc tattcgaatg
    15301 ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa atatccagtt
    15361 cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca atctataaaa gatatagtct
    15421 ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt ttgcagccac
    15481 ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg ataaaattct
    15541 ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt atctgcccaa
    15601 gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc atgcatcgca
    15661 gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat tacttctttt
    15721 ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat cccttgtgtt
    15781 tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc atctgtgaaa
    15841 gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt cattttttca
    15901 cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg gttttgaaaa
    15961 gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc atttttatgt
    16021 ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc tcattgtgtc
    16081 aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat catcgtccaa
    16141 cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt ggcagaagcc
    16201 ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa tttttcgacg
    16261 ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta cttcatggtc
    16321 cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt gcaggccggc
    16381 gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat
    16441 ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag tggtggcttg
    16501 tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat atccgttttt
    16561 tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat tccttttttg
    16621 agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt gacaaacgac
    16681 ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt ggagctaggc
    16741 gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca gtattagctc
    16801 aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt ttcggcttct
    16861 tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc ttctcgataa
    16921 agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc tgcaggataa
    16981 aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc gctgtcttgc
    17041 aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata gtagatcact
    17101 ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc
    17161 tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata ttcgatctct
    17221 actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg atttggtaca
    17281 tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc aaatggtttg
    17341 accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata aaatccccct
    17401 tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt gacagaaaaa
    17461 acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa cgtacttgat
    17521 caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg ttttcaccta
    17581 ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct gaatcttcaa
    17641 tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga aaaacttctg
    17701 gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa taacttgtca
    17761 aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga
    17821 tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg gatgtttcat
    17881 ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag gctgatttct
    17941 ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct agtgactcgg
    18001 ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg gacagctggt
    18061 tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga acgccatcta
    18121 aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa cgacttgaag
    18181 taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct gtaaagcttc
    18241 ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc
    18301 tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc cctctggtac
    18361 tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta gacgtccttc
    18421 gcctacgatt acgaggtctg cttgctgtac ccgtttatcg aactccagta aatccaatac
    18481 gtagtcgatg ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc
    18541 accaccagca ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc
    18601 atgcatggct tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa
    18661 aacaaaagaa gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat
    18721 tttggcttta atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag
    18781 attttctccc agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc
    18841 ttctgccatg ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg
    18901 tcgaacacct tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct
    18961 aagtggctgt tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa
    19021 aatcgcagtt tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt
    19081 aacgactgct gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc
    19141 tcctccatcg cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc
    19201 tgattgaatg gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc
    19261 aatcagaata cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat
    19321 tggtctccat tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt
    19381 ttttttgtca aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt
    19441 tcccatgctg aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct
    19501 gaaaaaccaa atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca
    19561 tctgatgaat gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac
    19621 gcccattcca tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat
    19681 gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa
    19741 aaatactcag gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt
    19801 tcatttactt cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg
    19861 taagtggtca aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt
    19921 tctttgttag gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa
    19981 agcggatcgc cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc
    20041 gtatatgcat tattgtcgat atattctgtg tattcatctg gaccaatgac tctgtgtatc
    20101 tctaaatgat catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg
    20161 agcgtttttc cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga
    20221 acggcataag caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt
    20281 cttgttccgg ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt
    20341 gcgctttccc atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg
    20401 ccttctaatc gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat
    20461 aaaaagaaag gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg
    20521 ccagttagtc ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt
    20581 aaatggtaag cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt
    20641 atccgatgct gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta
    20701 taactaacgc ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga
    20761 gaggtagaga gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct
    20821 gaaaaggaag cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta
    20881 gctgcaatcc caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg
    20941 tcaccaaata ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt
    21001 ccatcgatac ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact
    21061 ttcagggctg cctgatggag attattttga tcagcaacac gacaagtaat gacttccact
    21121 ttttttcctt gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct
    21181 agtgcccgct cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt
    21241 tttatttccc atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa
    21301 ggtcggttat atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc
    21361 tggcttcctc tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg
    21421 atatactcta aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt
    21481 ctgccgaacg atataaattt tccaccagat tctggatgac atatccttgc cagccatctg
    21541 caatcatctg agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat
    21601 gtgggtcatt aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga
    21661 atagttcgcc tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt
    21721 tgtcttctct gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta
    21781 attccagtgt gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga
    21841 atttccctga attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta
    21901 ctttaggaaa tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca
    21961 aaggcccgcc tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc
    22021 gacgaagcgc attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga
    22081 tcattgttgc ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt
    22141 tatcggctgt ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc
    22201 acatcactga agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg
    22261 gtgtacagac actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat
    22321 acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa
    22381 ccacagcggc caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt
    22441 ctgctacttg tccagcgcct atgattgcta ctttcttcat ttatttctcc ctcctttgct
    22501 tatttactta ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt
    22561 ttccagacag tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc
    22621 tcgcaaacga cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa
    22681 atggcgagta aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg
    22741 gatatgaccc acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat
    22801 attcatatga taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta
    22861 atgacgcgca tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa
    22921 ataaataacc gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac
    22981 tgctttatag tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa
    23041 cattccccaa gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa
    23101 aatctcagca atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca
    23161 cccatcgtag ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt
    23221 gactgtgtcg atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca
    23281 gtctttgata aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt
    23341 accgaatttc attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg
    23401 tgctggtcta cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca
    23461 atgatttctt cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt
    23521 cgtttacggt tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg
    23581 atttttgcat tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta
    23641 gcaaaggcaa cataagaaat gatcccgcca tagccgatcc ctcttaaaga agcttgtaat
    23701 gcggcagcac tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc
    23761 tttagacgaa aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtatttt
    23821 tctgctaatt ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct
    23881 tttttagcta attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca
    23941 tcacctgctc gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa
    24001 catactgcat ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca
    24061 ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga
    24121 tactctgtca ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga
    24181 aattcgccaa ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat
    24241 tcttctgaaa attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt
    24301 cctggttttg gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg
    24361 tcttcgtaat ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt
    24421 tctttcctct ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat
    24481 aaatcgttca gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc
    24541 catcattcgt ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa
    24601 agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac
    24661 cataaagcaa taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg
    24721 aataatgatc tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg
    24781 aatcgtgtca aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat
    24841 cgtgataatc agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa
    24901 tggaacaacg atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt
    24961 cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac
    25021 tgcagttacg gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg
    25081 aaaaatctca gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat
    25141 agtttgtgtc gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa
    25201 aaatggaaac aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct
    25261 gctcttttta tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca
    25321 ataatgatga acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag
    25381 gctgtctgat acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct
    25441 gtaaacatat agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg
    25501 attgccggca gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct
    25561 gcttcataaa gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac
    25621 ggatagtatc tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg
    25681 aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt
    25741 ccataactat tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtaaca
    25801 taacttaata aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa
    25861 atagctacgg ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact
    25921 gttaaaccta aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat
    25981 ccgataaaaa cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata
    26041 ttggaaagca ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt
    26101 cccatggcta attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga
    26161 taatctgcca atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc
    26221 gtattttttt gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg
    26281 ttcactgctt ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt
    26341 tccccgacta gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc
    26401 tcgccaaaag acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta
    26461 ttcactggtt gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga
    26521 gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa
    26581 atcgtcaatc cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa
    26641 ccgatatttt ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt
    26701 gccattggtg ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct
    26761 ggcatcgtgt attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta
    26821 tcgatcgtta cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga
    26881 gagaacgctt gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta
    26941 ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct
    27001 gaaaaaccag cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta
    27061 attggtgcag caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca
    27121 taataattat cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt
    27181 tcatctttat ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta
    27241 ccggaacgag ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc
    27301 ttgattttga tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt
    27361 aatcgttcct gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta
    27421 gagctttgac tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta
    27481 acaataattt ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc
    27541 atacggctgg aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag
    27601 gtctaggtaa tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat
    27661 aactaagact ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc
    27721 cagtaagtaa atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat
    27781 ctgggtcaat tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat
    27841 atctgctaac aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc
    27901 tttgtaatca tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa
    27961 cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt
    28021 tggagaactt aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc
    28081 tgggtttttc ttgtagttta ctcgggtacc ctctttcttc agctgttcca ctaattcaag
    28141 gatcgttttt tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc
    28201 taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt
    28261 caacttactt gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg
    28321 atagaccatc tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg
    28381 cacatttgtc tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa
    28441 taattggatg attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca
    28501 taaaaatccc acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag
    28561 aatatcgatc attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc
    28621 gctaaaagtc gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa
    28681 cgggctggtc cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa
    28741 aaaatctttg tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga
    28801 catatgattg cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc
    28861 ttcccatgtg cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc
    28921 agatgtatat gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt
    28981 ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc
    29041 ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt
    29101 ctgctgataa acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga
    29161 gtcgcgataa atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga
    29221 gcgttcccaa cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa
    29281 ataattttga tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct
    29341 tcttaaaaaa atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata
    29401 gacaaaaaag aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt
    29461 gcatataaaa ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc
    29521 aactgtctcc agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt
    29581 acttcaagca atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg
    29641 aaatggtttt gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa
    29701 tccttttttt tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga
    29761 cttgattcta caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat
    29821 ccaagaaaaa atgatccgag gaattattat tttaagctct acgatggatg aaacacttct
    29881 ttttgaattg gatcaataca atattcctat tgtggtaatc ggaaaagtcg gaaacgacta
    29941 cacgaacatc tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac
    30001 actgatcaag aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt
    30061 atccaatgat cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg
    30121 taatgaatgg attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga
    30181 tttaatgaag ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt
    30241 gagtatttac aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc
    30301 aggattcagt gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat
    30361 tccgacaaat cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca
    30421 ataccctgaa gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca
    30481 acaacaagaa atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta
    30541 ctctgttctt ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt
    30601 ttcttatttt ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat
    30661 tttttccaca gggaagaaaa aactattttt aaccacgttg tgtataagtt tttatagttc
    30721 gtttttttaa acacatttac ccttatttca atccatgtta tttggggata cttatctcta
    30781 aaacataaat agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa
    30841 taatagctag caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt
    30901 caaaaaatga tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga
    30961 taccttaatt ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag
    31021 acaaattatt ttagac
//
LOCUS       DAYYAJ010000019        30901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000019 DAYYAJ010000000
VERSION     DAYYAJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.019"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHATZ_000753"
     CDS             complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHATZ_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8403306.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1699..1827)
                     /locus_tag="ACHATZ_000754"
     CDS             complement(1699..1827)
                     /locus_tag="ACHATZ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403307.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2172..2315)
                     /locus_tag="ACHATZ_000755"
     CDS             complement(2172..2315)
                     /locus_tag="ACHATZ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8403308.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2538..3017)
                     /locus_tag="ACHATZ_000756"
     CDS             complement(2538..3017)
                     /locus_tag="ACHATZ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8403309.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3090..3374)
                     /locus_tag="ACHATZ_000757"
     CDS             complement(3090..3374)
                     /locus_tag="ACHATZ_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403310.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3452..3646)
                     /gene="ssrS"
                     /locus_tag="ACHATZ_000758"
     ncRNA           complement(3452..3646)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHATZ_000758"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3718..5004)
                     /locus_tag="ACHATZ_000759"
     CDS             complement(3718..5004)
                     /locus_tag="ACHATZ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8403311.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5142..5813
                     /locus_tag="ACHATZ_000760"
     CDS             5142..5813
                     /locus_tag="ACHATZ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8403312.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5779..6267
                     /locus_tag="ACHATZ_000761"
     CDS             5779..6267
                     /locus_tag="ACHATZ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8403313.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6280..7227
                     /gene="prmA"
                     /locus_tag="ACHATZ_000762"
     CDS             6280..7227
                     /gene="prmA"
                     /locus_tag="ACHATZ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8403314.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7228..7992
                     /locus_tag="ACHATZ_000763"
     CDS             7228..7992
                     /locus_tag="ACHATZ_000763"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8403315.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8225..10438
                     /locus_tag="ACHATZ_000764"
     CDS             8225..10438
                     /locus_tag="ACHATZ_000764"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8403316.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10449..10895
                     /gene="dtd"
                     /locus_tag="ACHATZ_000765"
     CDS             10449..10895
                     /gene="dtd"
                     /locus_tag="ACHATZ_000765"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8403317.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11066..12325
                     /locus_tag="ACHATZ_000766"
     CDS             11066..12325
                     /locus_tag="ACHATZ_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8403318.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12414..13361)
                     /locus_tag="ACHATZ_000767"
     CDS             complement(12414..13361)
                     /locus_tag="ACHATZ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8403319.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13755..15047
                     /gene="hisS"
                     /locus_tag="ACHATZ_000768"
     CDS             13755..15047
                     /gene="hisS"
                     /locus_tag="ACHATZ_000768"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8403320.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15062..16831
                     /gene="aspS"
                     /locus_tag="ACHATZ_000769"
     CDS             15062..16831
                     /gene="aspS"
                     /locus_tag="ACHATZ_000769"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8403321.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16940..17815
                     /locus_tag="ACHATZ_000770"
     CDS             16940..17815
                     /locus_tag="ACHATZ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8403322.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18081..19964
                     /locus_tag="ACHATZ_000771"
     CDS             18081..19964
                     /locus_tag="ACHATZ_000771"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8403323.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20039..20323)
                     /locus_tag="ACHATZ_000772"
     CDS             complement(20039..20323)
                     /locus_tag="ACHATZ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8403324.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20313..21047)
                     /locus_tag="ACHATZ_000773"
     CDS             complement(20313..21047)
                     /locus_tag="ACHATZ_000773"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8403325.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21176..21799
                     /locus_tag="ACHATZ_000774"
     CDS             21176..21799
                     /locus_tag="ACHATZ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8403326.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            21991..23112
                     /locus_tag="ACHATZ_000775"
     CDS             21991..23112
                     /locus_tag="ACHATZ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8403327.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23119..26247
                     /locus_tag="ACHATZ_000776"
     CDS             23119..26247
                     /locus_tag="ACHATZ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8403328.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26266..29460
                     /locus_tag="ACHATZ_000777"
     CDS             26266..29460
                     /locus_tag="ACHATZ_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8403329.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29606..30313)
                     /locus_tag="ACHATZ_000778"
     CDS             complement(29606..30313)
                     /locus_tag="ACHATZ_000778"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8403330.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10119 a         5589 c         6528 g         8665 t
ORIGIN      
        1 aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa
       61 taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga
      121 gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt
      181 gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg
      241 ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat
      301 aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg
      361 cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga
      421 tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata
      481 atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac
      541 gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt
      601 tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat
      661 ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt
      721 tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa
      781 tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag
      841 ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg
      901 caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag
      961 aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt
     1021 taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa
     1081 ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct
     1141 gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac
     1201 cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt
     1261 ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta
     1321 tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt
     1381 gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt
     1441 ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaaga
     1501 caattttttc accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga
     1561 ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa
     1621 aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc
     1681 ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac
     1741 agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt
     1801 acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc
     1861 cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg
     1921 atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac
     1981 atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa
     2041 ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc
     2101 tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg
     2161 gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac
     2221 ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca
     2281 tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata agctttggca
     2341 tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt
     2401 attttacttg aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag
     2461 tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt
     2521 ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa
     2581 tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa
     2641 ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact
     2701 tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca
     2761 gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc
     2821 tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa
     2881 gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat agctacattg
     2941 accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt
     3001 ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg
     3061 tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca
     3121 aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata
     3181 aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct
     3241 ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata
     3301 tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc gataatcatt
     3361 aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc
     3421 aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc
     3481 tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc
     3541 aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat
     3601 gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag
     3661 catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta
     3721 gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg
     3781 ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa
     3841 atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg
     3901 gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc
     3961 tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt
     4021 tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc
     4081 caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa
     4141 acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc
     4201 ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt
     4261 ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt
     4321 tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga
     4381 aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt
     4441 tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc
     4501 gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg
     4561 tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt
     4621 ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc
     4681 ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat
     4741 ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat
     4801 gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc
     4861 aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat
     4921 ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat
     4981 gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat
     5041 tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc
     5101 ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga
     5161 tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt
     5221 atttggagca tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat
     5281 acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc
     5341 gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt
     5401 tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac
     5461 ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca
     5521 gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt
     5581 ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca
     5641 atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt
     5701 ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt
     5761 ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt
     5821 gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc
     5881 gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa
     5941 gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct
     6001 cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa
     6061 gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa
     6121 gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt
     6181 cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat
     6241 acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa
     6301 gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg
     6361 agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt
     6421 gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat
     6481 tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa
     6541 ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa
     6601 agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg
     6661 acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca
     6721 ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca
     6781 gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc
     6841 gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac
     6901 gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc
     6961 catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg
     7021 aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa
     7081 acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc
     7141 atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt
     7201 attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa
     7261 tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg
     7321 atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa
     7381 atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa
     7441 aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat
     7501 tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca
     7561 gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag
     7621 attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg
     7681 gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat
     7741 gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa
     7801 ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc
     7861 gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca
     7921 gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct
     7981 tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag
     8041 tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa
     8101 aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca
     8161 attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat
     8221 tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata
     8281 catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca
     8341 tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg
     8401 tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt
     8461 tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc
     8521 tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca
     8581 gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat
     8641 ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa
     8701 gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg
     8761 gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca
     8821 gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag
     8881 tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta
     8941 tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt
     9001 caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta
     9061 tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta
     9121 tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc
     9181 aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg
     9241 ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat
     9301 gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc
     9361 ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac
     9421 accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag
     9481 tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca
     9541 gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt
     9601 tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa
     9661 acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt
     9721 caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca
     9781 agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta
     9841 tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca
     9901 aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc
     9961 tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact
    10021 aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac
    10081 taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc
    10141 aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga
    10201 gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact
    10261 tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga
    10321 taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat
    10381 cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga
    10441 ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc
    10501 aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata
    10561 cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg
    10621 aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc
    10681 aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac
    10741 gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca
    10801 tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac
    10861 ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa
    10921 actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc
    10981 tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt
    11041 tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac
    11101 agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc
    11161 ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc
    11221 atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa
    11281 attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca
    11341 tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca
    11401 tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact
    11461 atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt
    11521 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
    11581 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
    11641 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
    11701 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
    11761 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
    11821 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
    11881 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
    11941 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
    12001 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
    12061 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
    12121 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
    12181 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
    12241 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
    12301 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
    12361 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
    12421 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
    12481 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
    12541 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
    12601 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
    12661 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
    12721 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
    12781 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
    12841 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
    12901 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
    12961 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
    13021 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
    13081 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
    13141 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
    13201 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
    13261 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
    13321 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
    13381 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
    13441 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
    13501 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
    13561 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
    13621 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
    13681 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
    13741 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
    13801 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
    13861 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
    13921 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
    13981 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
    14041 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
    14101 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
    14161 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
    14221 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
    14281 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
    14341 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
    14401 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
    14461 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
    14521 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
    14581 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
    14641 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
    14701 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
    14761 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
    14821 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
    14881 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
    14941 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
    15001 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
    15061 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
    15121 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
    15181 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
    15241 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
    15301 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
    15361 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
    15421 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
    15481 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
    15541 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
    15601 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
    15661 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
    15721 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
    15781 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
    15841 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
    15901 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
    15961 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
    16021 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
    16081 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
    16141 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
    16201 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
    16261 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
    16321 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
    16381 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
    16441 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
    16501 cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
    16561 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
    16621 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
    16681 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
    16741 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
    16801 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
    16861 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
    16921 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
    16981 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
    17041 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
    17101 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
    17161 tataccttta tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
    17221 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
    17281 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
    17341 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
    17401 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
    17461 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
    17521 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
    17581 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
    17641 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
    17701 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
    17761 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
    17821 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
    17881 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
    17941 gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa
    18001 ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt
    18061 atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca
    18121 ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc
    18181 caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt
    18241 tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat
    18301 tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt
    18361 cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca
    18421 gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc
    18481 gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact
    18541 atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga
    18601 tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata
    18661 caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat
    18721 ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga
    18781 aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt
    18841 gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga
    18901 gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat
    18961 ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc
    19021 agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt
    19081 tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg
    19141 tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt
    19201 tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag
    19261 caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac
    19321 aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag
    19381 aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac
    19441 aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg
    19501 ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac
    19561 aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca
    19621 ctgttctgat cggactagga acgaatggct cattctcgga agctcagttt gatagtgtga
    19681 tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct
    19741 ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc
    19801 tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc
    19861 atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag
    19921 gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt
    19981 caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc
    20041 atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc
    20101 gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt
    20161 ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac
    20221 cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct
    20281 gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc
    20341 tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg
    20401 tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt
    20461 gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt
    20521 tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta
    20581 cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg
    20641 tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg
    20701 actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg
    20761 acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga
    20821 cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca
    20881 actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag
    20941 tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg
    21001 atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa
    21061 aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg
    21121 tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag agactatgtt
    21181 ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg
    21241 ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg
    21301 tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat
    21361 ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc
    21421 ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt
    21481 gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact
    21541 taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata
    21601 tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg
    21661 cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga
    21721 acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata
    21781 tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt
    21841 cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt
    21901 ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat
    21961 aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat
    22021 atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct
    22081 atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat
    22141 gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg
    22201 aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga
    22261 ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca
    22321 gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa
    22381 ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta
    22441 gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca agtattgatt
    22501 agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc
    22561 gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc actaggacat
    22621 ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc cccattgaaa
    22681 ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc
    22741 ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt
    22801 ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa ttacatctat
    22861 atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg gcaaatatat
    22921 cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga
    22981 aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag tgaaatgacg
    23041 gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca agaaatcatg
    23101 gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt
    23161 atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt gatcagcggg
    23221 aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact ttttggtgaa
    23281 acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac accagacgaa
    23341 gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat cgaacgaaaa
    23401 ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc agccaaagta
    23461 acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa
    23521 gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc tcaaatcgtt
    23581 ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga taaagagact
    23641 gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg attaaaagaa
    23701 aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca tcttctgcaa
    23761 aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc cgttttagcc
    23821 caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa agaagctcta
    23881 acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt tgaaagcttg
    23941 aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt agctgctcaa
    24001 aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac gcaacgatta
    24061 gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact gtcacaaaaa
    24121 agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga gtggcaaaaa
    24181 aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa
    24241 aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa
    24301 caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta tcaaaatctc
    24361 gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca ggaaaaactg
    24421 caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca gcaagaaata
    24481 gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga actcaaaaaa
    24541 gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga acaagcagaa
    24601 cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca
    24661 ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt aaaacacgaa
    24721 cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc tgagtctgct
    24781 gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa tcttcaacaa
    24841 caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc tgaacaagaa
    24901 gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa
    24961 acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat ggccaatgca
    25021 agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca attagctgtc
    25081 tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg agaaatcaaa
    25141 agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga gatacgacga
    25201 cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa agcaggagtg
    25261 caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca tgaacaaata
    25321 gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc
    25381 cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca agcacaatat
    25441 aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac agctgaccgg
    25501 ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga agagatcagc
    25561 caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat atacggttta
    25621 caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca
    25681 agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac attcaacgga
    25741 gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct tcaagaagtg
    25801 ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca atttgagcta
    25861 gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat ctatgatgat
    25921 caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt tattgcagca
    25981 ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg tgtagcaatc
    26041 gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt ggaaatggca
    26101 atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta
    26161 agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg tagcggacaa
    26221 agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa atggagtatt
    26281 ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt gttatcggtt
    26341 gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga attgtatatg
    26401 gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta ttgggaggaa
    26461 cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa aaatctatct
    26521 cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag tgagggagaa
    26581 atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct
    26641 ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc agagctttct
    26701 attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac atatatcgta
    26761 aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac tgttaataaa
    26821 cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa aatactcacg
    26881 catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt acaagtcaaa
    26941 ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt cctgttagag
    27001 cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga tcaaggaata
    27061 caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg agaagattat
    27121 ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg gggagttatc
    27181 caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac ttggaaacaa
    27241 ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt accgtctttg
    27301 tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc ccctgcagta
    27361 gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa agcaaaagga
    27421 aaaatccaag ctcgagtcga tttcgtctat ggcgaagtca tctattcgac tgacccgaag
    27481 catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga agagaaacga
    27541 gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta tgaacgtcca
    27601 ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt cttccgtcag
    27661 tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc gcataaacat
    27721 caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt cgatgtgaca
    27781 gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt
    27841 tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca acaaacaagc
    27901 caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat ccatgtacct
    27961 aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa
    28021 tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc acagcttccc
    28081 aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg gttgaagatg
    28141 ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg gaaaacacta
    28201 cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc
    28261 gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt cgccccttct
    28321 ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact ggctaaagac
    28381 acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa ttatcagtcg
    28441 caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa
    28501 actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag tgggacacca
    28561 atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc aggatttttc
    28621 ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg
    28681 tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa aacagagatg
    28741 aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta cttgcgtcaa
    28801 atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg gatcgggata
    28861 cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat
    28921 taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc tgccaaagag
    28981 aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat cttgcaagaa
    29041 gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa accacaagat
    29101 cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct gatttcctta
    29161 aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt gtatgacctt
    29221 tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat cggacaaaaa
    29281 aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg catggattca
    29341 ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga agaacagttg
    29401 acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat cgaggagtaa
    29461 gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag cgattgttta
    29521 tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt ccttgctatc
    29581 atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc tctaacagct
    29641 gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg cgttctcgat
    29701 ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca ttgtcgtaat
    29761 tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca gctagctgaa
    29821 aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata ttgcgggaag
    29881 gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca gtcggcccat
    29941 ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt tttttgacct
    30001 cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat aagaggccat
    30061 cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc cgatgtgctc
    30121 gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca tcactatgat
    30181 ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga acgactcttt
    30241 tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca tcaaacacaa
    30301 ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca caaatccttg
    30361 actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt tcttactata
    30421 tttctatttc cttgacagaa taaggttttt atatttcagt ttttttcact aaaaatcgtg
    30481 aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg attgatttat
    30541 tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatacag tctcaatata
    30601 actattcaaa cataaaataa cgttatttga tttatttgtt tatagtaaat tttatttagt
    30661 aatcattgct ttaaaatact acgcttatat aacagagaaa ataagcgtta taataaagaa
    30721 cagtattatt taaaatgaca gaaaacatta ctattaaaga aataaaaaac ataaaaaatc
    30781 ttaagaaatc ggtttcattt agcttttgag ataaaataat agtacagttt tatacgctgt
    30841 ttcatatgtt tagaaaattt gaattagatt tgaaaactct aatttttaaa tgataaaaaa
    30901 a
//
LOCUS       DAYYAJ010000020        29560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000020 DAYYAJ010000000
VERSION     DAYYAJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.020"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..2155)
                     /locus_tag="ACHATZ_000779"
     CDS             complement(77..2155)
                     /locus_tag="ACHATZ_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8403331.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(2168..2443)
                     /locus_tag="ACHATZ_000780"
     CDS             complement(2168..2443)
                     /locus_tag="ACHATZ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403332.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2805..3260)
                     /locus_tag="ACHATZ_000781"
     CDS             complement(2805..3260)
                     /locus_tag="ACHATZ_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403333.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3414..4622)
                     /locus_tag="ACHATZ_000782"
     CDS             complement(3414..4622)
                     /locus_tag="ACHATZ_000782"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8403334.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4734..5066)
                     /locus_tag="ACHATZ_000783"
     CDS             complement(4734..5066)
                     /locus_tag="ACHATZ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8403335.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5214..6089
                     /locus_tag="ACHATZ_000784"
     CDS             5214..6089
                     /locus_tag="ACHATZ_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8403336.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(6171..6752)
                     /locus_tag="ACHATZ_000785"
     CDS             complement(6171..6752)
                     /locus_tag="ACHATZ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8403337.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6758..8017)
                     /locus_tag="ACHATZ_000786"
     CDS             complement(6758..8017)
                     /locus_tag="ACHATZ_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8403338.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(8019..9065)
                     /locus_tag="ACHATZ_000787"
     CDS             complement(8019..9065)
                     /locus_tag="ACHATZ_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403339.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9300..9575)
                     /locus_tag="ACHATZ_000788"
     CDS             complement(9300..9575)
                     /locus_tag="ACHATZ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8403340.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9818..11128)
                     /gene="der"
                     /locus_tag="ACHATZ_000789"
     CDS             complement(9818..11128)
                     /gene="der"
                     /locus_tag="ACHATZ_000789"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8403341.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(11310..12539)
                     /gene="rpsA"
                     /locus_tag="ACHATZ_000790"
     CDS             complement(11310..12539)
                     /gene="rpsA"
                     /locus_tag="ACHATZ_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8403342.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(12674..13354)
                     /gene="cmk"
                     /locus_tag="ACHATZ_000791"
     CDS             complement(12674..13354)
                     /gene="cmk"
                     /locus_tag="ACHATZ_000791"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8403343.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(13403..14038)
                     /locus_tag="ACHATZ_000792"
     CDS             complement(13403..14038)
                     /locus_tag="ACHATZ_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8403344.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(14104..15507)
                     /locus_tag="ACHATZ_000793"
     CDS             complement(14104..15507)
                     /locus_tag="ACHATZ_000793"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8403345.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(15494..16525)
                     /locus_tag="ACHATZ_000794"
     CDS             complement(15494..16525)
                     /locus_tag="ACHATZ_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403346.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            16556..16777
                     /locus_tag="ACHATZ_000795"
     CDS             16556..16777
                     /locus_tag="ACHATZ_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8403347.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(16942..17679)
                     /locus_tag="ACHATZ_000796"
     CDS             complement(16942..17679)
                     /locus_tag="ACHATZ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8403348.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(17832..19172)
                     /locus_tag="ACHATZ_000797"
     CDS             complement(17832..19172)
                     /locus_tag="ACHATZ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8403349.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(19409..20932)
                     /locus_tag="ACHATZ_000798"
     CDS             complement(19409..20932)
                     /locus_tag="ACHATZ_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8403350.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(21311..21922)
                     /locus_tag="ACHATZ_000799"
     CDS             complement(21311..21922)
                     /locus_tag="ACHATZ_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8403351.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(22045..22170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(22252..22968)
                     /locus_tag="ACHATZ_000800"
     CDS             complement(22252..22968)
                     /locus_tag="ACHATZ_000800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8403352.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(22974..23543)
                     /gene="scpB"
                     /locus_tag="ACHATZ_000801"
     CDS             complement(22974..23543)
                     /gene="scpB"
                     /locus_tag="ACHATZ_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8403353.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(23527..24297)
                     /locus_tag="ACHATZ_000802"
     CDS             complement(23527..24297)
                     /locus_tag="ACHATZ_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8403354.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(24294..24665)
                     /locus_tag="ACHATZ_000803"
     CDS             complement(24294..24665)
                     /locus_tag="ACHATZ_000803"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8403355.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(24684..25571)
                     /gene="xerD"
                     /locus_tag="ACHATZ_000804"
     CDS             complement(24684..25571)
                     /gene="xerD"
                     /locus_tag="ACHATZ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8403356.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(25667..26140)
                     /locus_tag="ACHATZ_000805"
     CDS             complement(25667..26140)
                     /locus_tag="ACHATZ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8403357.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(26245..27108)
                     /locus_tag="ACHATZ_000806"
     CDS             complement(26245..27108)
                     /locus_tag="ACHATZ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8403358.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(27304..29403)
                     /locus_tag="ACHATZ_000807"
     CDS             complement(27304..29403)
                     /locus_tag="ACHATZ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8403359.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         8222 a         6271 c         4965 g        10102 t
ORIGIN      
        1 caaaaaaaga acaggaccga ttcgatcctg ttctgatatg ttttgttttt tataaaactt
       61 gaaaattttt acctttttat tttattggac tcgttttttt tgactttgct cgaaacgcaa
      121 atcggaaaga aaagttatca gatggtttgt tcatcgtata agattctaac aaattgatat
      181 tcctgcgata gctgttcgca gaaaacgtcg tgattttttc agcaacttcg tattcatcaa
      241 tgatttttct ttcaagataa taacctctta gcattttttt cgtaaacaga ctatcttgct
      301 ggatcatcca cgaagaaatc aaattccccg agttcatttt tattccttca agtttgcgtt
      361 gctggataaa aaattgaacc atcgtttcat cataaatatc agtcaagtta tctaaacttt
      421 caataatcaa ctgagtattt ttttgatatg tctctctgat ttcttggata tcttcatcac
      481 taataaatgt ctgacgatca gcatatcttc tttgccaaaa cattttcgga tggagaacga
      541 ttcgcattaa cctacggaca acaatgaacc aaaagccgat aaaagacaaa atctggcttg
      601 tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa acgataacca ttagaagaaa
      661 gcttgcccga acgataactt tcatccaatc catcccgttc gatcgacaaa atcagtgctt
      721 gtatttcttg tacttcttgc ttttcatcat cggttaaatc ttccaagtac agatcacgta
      781 tccgttcttg ataattttct atcactgctt cagtggctaa aaattctttt gtattacgat
      841 caatttcctt tatttctttt cgcaaaactt caacgacctc ttctaaaatc atcaaagcat
      901 tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat aggcaagaca atgatcccta
      961 tgaccaaagt cactaagatg acacccgccg tcagaaacaa aagcaaagac cgctggtaaa
     1021 acatcatatt attgattgaa aatggcaaaa taaaaatcgt agccaaactg actgttccct
     1081 ttacaccgcc aaatgttaag atcaagatct cgttcaactg ttttttgaac tttttactgc
     1141 catctttgaa tacataaaaa agactgatag aaatgaatct gatcacaaaa agcatgacag
     1201 agattaatac gataatcgct aataaaagac cgtttggata taaaccgtct ccccatactg
     1261 gcgagaacac ttgcgtcaat tcgattccta aaaatataaa tactaatgca ttcagtgtaa
     1321 aagcaatagt tgtccatgta ctgtgggaca agctcgacaa ttctgcatca aacacagaga
     1381 ttttcctaaa accagaagct tggagaatac ctgctgccac tgcagcaatg attccagata
     1441 catcaaatgc ctctgccaat acataggcta gaaaaggaag caaaagttcg atcagcagat
     1501 accctgtgac atcttgtgct gatgcttttt cgatcaaatg aattattttt tgtttgaacc
     1561 agacaagcaa aaagccgatc aaagctcccc caatactaga aactactaga ctcatccccg
     1621 cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag agcaaactgg aaggcagtaa
     1681 caccagatgc atcattcaaa agaccttctc cttccaaaat atgcatcgcc tttttaggga
     1741 tattcactcg tccagaaaga gaactgactg ctaccgcatc tgtcggacct aacgctgctc
     1801 caaaagcaaa gcaggcagca agtgggacac ttggcaacag gaatgataac gtcgcaccta
     1861 ccccgacaag ggtcaaaatg actcctccaa atgccaagaa caaaattgtc ccgaaatttt
     1921 tgagaatcga agaaatatct gctttttctc cttctctaaa taacaatggt gcaataatca
     1981 taactaagaa caattcaggt tcaaaatcga ttgattgtcc ccactctgtc agacctaata
     2041 ccactccaag gaaaatttgg atcagtggag ctggaatcat tggcaaaatg cggctagcta
     2101 tgttcgataa ggtgatcgtt actgcaaata caataactaa ctcagcaaat tccatcgtcc
     2161 atccccttta ttttttattt tgtgtaagca tttttaaaaa ttgttctttt ttctttaaaa
     2221 ttttggtttc gactacttca acctgtgttt ggttcccctc taattcacac tgtacacgtt
     2281 ttgactccaa acgaagcaga tcatgtttca gacgttccaa ctgacgatac atcaatatat
     2341 ttcttttacg caacttacaa agtatttgac ggcggtcatc catcgaaagg gcgccatagc
     2401 gatcatgcaa cagttttttt tgttgttcaa aactctctct cattttccag atctcctata
     2461 cacaaacttc catcattata gcaaagttct ttttggatga aaaatatttc attgtttcat
     2521 cgtttattgc tagttagaaa tttccataaa tttttcaacg cctcattcta ttccaaatag
     2581 tatcagatat caagtaaaac ttaagaaaga taaaagtttc tgacaaaagg atgtttactt
     2641 tcccattcct gcctttcttg ttcttttatc acaacaaaaa aatggggctg cgacaaaaat
     2701 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
     2761 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
     2821 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
     2881 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
     2941 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
     3001 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
     3061 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
     3121 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
     3181 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
     3241 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
     3301 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
     3361 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
     3421 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
     3481 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
     3541 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
     3601 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
     3661 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     3721 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     3781 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     3841 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     3901 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     3961 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     4021 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     4081 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     4141 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     4201 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     4261 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     4321 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     4381 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     4441 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     4501 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     4561 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     4621 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     4681 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     4741 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     4801 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     4861 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     4921 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     4981 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     5041 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     5101 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctat atttaggaag
     5161 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     5221 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     5281 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     5341 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     5401 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     5461 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     5521 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     5581 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     5641 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     5701 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     5761 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     5821 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     5881 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     5941 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     6001 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     6061 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     6121 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     6181 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     6241 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     6301 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     6361 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     6421 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     6481 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     6541 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     6601 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     6661 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     6721 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     6781 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     6841 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     6901 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     6961 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     7021 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     7081 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     7141 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     7201 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     7261 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     7321 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     7381 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     7441 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     7501 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     7561 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     7621 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     7681 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     7741 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     7801 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     7861 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     7921 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     7981 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     8041 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     8101 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     8161 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     8221 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     8281 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     8341 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     8401 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     8461 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     8521 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     8581 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     8641 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     8701 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     8761 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     8821 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     8881 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     8941 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     9001 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     9061 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     9121 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     9181 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     9241 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     9301 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     9361 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     9421 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     9481 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     9541 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     9601 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     9661 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     9721 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     9781 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     9841 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     9901 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     9961 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
    10021 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
    10081 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
    10141 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
    10201 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
    10261 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
    10321 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
    10381 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
    10441 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
    10501 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
    10561 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
    10621 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
    10681 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
    10741 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
    10801 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
    10861 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
    10921 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
    10981 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
    11041 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
    11101 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
    11161 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
    11221 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
    11281 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
    11341 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
    11401 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
    11461 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
    11521 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
    11581 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
    11641 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
    11701 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
    11761 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
    11821 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
    11881 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
    11941 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
    12001 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
    12061 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
    12121 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
    12181 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
    12241 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
    12301 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
    12361 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
    12421 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
    12481 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
    12541 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
    12601 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
    12661 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    12721 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    12781 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    12841 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    12901 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    12961 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    13021 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    13081 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    13141 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    13201 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    13261 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    13321 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    13381 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    13441 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    13501 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    13561 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    13621 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    13681 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    13741 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    13801 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    13861 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    13921 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    13981 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    14041 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    14101 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    14161 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    14221 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    14281 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    14341 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    14401 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    14461 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    14521 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    14581 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    14641 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    14701 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    14761 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    14821 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    14881 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    14941 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    15001 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    15061 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    15121 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    15181 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    15241 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    15301 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    15361 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    15421 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    15481 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    15541 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    15601 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    15661 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    15721 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    15781 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    15841 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    15901 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    15961 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    16021 aaaacagctg caccaaactc tcatacgcag tatcaattaa aatttcacgt ggcaattggc
    16081 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    16141 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    16201 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    16261 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    16321 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    16381 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    16441 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    16501 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    16561 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    16621 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    16681 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    16741 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    16801 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    16861 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    16921 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    16981 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    17041 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    17101 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    17161 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    17221 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    17281 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    17341 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    17401 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    17461 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    17521 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    17581 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    17641 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    17701 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    17761 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    17821 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    17881 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    17941 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    18001 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    18061 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    18121 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    18181 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    18241 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    18301 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    18361 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    18421 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    18481 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    18541 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    18601 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    18661 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    18721 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    18781 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    18841 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    18901 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    18961 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    19021 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    19081 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    19141 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    19201 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    19261 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    19321 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    19381 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    19441 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    19501 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    19561 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    19621 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    19681 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    19741 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    19801 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    19861 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    19921 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    19981 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    20041 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    20101 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    20161 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    20221 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    20281 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    20341 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    20401 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    20461 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    20521 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    20581 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    20641 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    20701 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    20761 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    20821 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    20881 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    20941 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    21001 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    21061 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    21121 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    21181 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    21241 tacattattg catgtcttgt atttttttcc aaaaaaaaaa gaccaagaaa tttcttgatc
    21301 tttttgatat ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    21361 accatggaag aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga
    21421 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct
    21481 gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    21541 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    21601 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    21661 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    21721 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    21781 tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    21841 gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt
    21901 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    21961 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    22021 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    22081 ggaatttcac cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc
    22141 gggaatttca ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta
    22201 tacatatcta ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga
    22261 gtggttcagc aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag
    22321 gccttgtaaa gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac
    22381 tgcttgaagc atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat
    22441 actgtggttt gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg
    22501 tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt
    22561 tacctttgca acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa
    22621 atctccatca tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg
    22681 atagatacgt tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc
    22741 tgcaacagca gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg
    22801 ataaattggg acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc
    22861 ccttacaact tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg
    22921 acgagaagtg atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct
    22981 cctttattca ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc
    23041 atttcttgtg tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag
    23101 ccagttgttc catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc
    23161 agtttatggg cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg
    23221 atttcagctc ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct
    23281 tgagataatg tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt
    23341 ttagtcgtta atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt
    23401 tgagtgtaag attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt
    23461 aacagtccgc ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct
    23521 tcaagttcac ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga
    23581 ttcatagttt tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc
    23641 cataaaagta gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca
    23701 gccatgcgca tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt
    23761 gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt
    23821 ctgcatggca cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc
    23881 atccgctaac ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc
    23941 tttttctgtc agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac
    24001 caattcttct cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac
    24061 aggaagcaat gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc
    24121 tgcaacagct agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat
    24181 cgtagcgatc ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag
    24241 atcgagagga ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct
    24301 ttcttctttt tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg
    24361 atggagacat ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca
    24421 ccgcgataag acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag
    24481 taaataccaa taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca
    24541 taatcgtctt cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt
    24601 ttcttatgga aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt
    24661 aacatcttct caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc
    24721 attcttttct tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac
    24781 aattcctgta ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc
    24841 cgcagcgtat gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga
    24901 tttttccaga ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt
    24961 tcagatgcgt cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg
    25021 atcgcataat cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc
    25081 agtccaagag aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc
    25141 ccagtagcat acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta
    25201 tcaggtgctt caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt
    25261 gctttcttcg gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt
    25321 aaaaactgat ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt
    25381 ttttcttcat gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat
    25441 gaagcaattt tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt
    25501 tgtcgtgtat tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt
    25561 tgctctttca taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg
    25621 atagtctttt tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt
    25681 ttcttttttc tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt
    25741 ttactaaaaa atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt
    25801 ctacttcctc gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc
    25861 cttcttttct caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt
    25921 tcaattctgt caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct
    25981 tttttttcac taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa
    26041 gcacaagcag agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct
    26101 gttttgtttt tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt
    26161 ataataatta caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag
    26221 ttaagaaaaa catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt
    26281 ttcttgtgta atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt
    26341 gctgatacca aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg
    26401 ctgatctgca gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc
    26461 atatgctctt ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg
    26521 accttttatc tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc
    26581 aagatagaaa tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc
    26641 tatcacatct ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt
    26701 ttcatctgct aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa
    26761 gcgatcccct tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac
    26821 aaagtcttta tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt
    26881 aacgattcca aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt
    26941 ttcatgtttt tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc
    27001 ttctgatttg cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt
    27061 ttcatcaata ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct
    27121 ctatagtgaa aggttttctt aatacatttc tttgctatca taacataatg ttatctactt
    27181 gaatagctga gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata
    27241 ctcccctttt tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta
    27301 aaattaattg ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta
    27361 aaatggcaaa gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata
    27421 tagattataa tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc
    27481 aacgatatat ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca
    27541 cgtccatgct ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg
    27601 attcgctgag tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc
    27661 gaccgcagct tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg
    27721 ttcttcagct gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc
    27781 tttatcagtg agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc
    27841 tgtatttttc actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga
    27901 atccactact tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc
    27961 agcatattga tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt
    28021 atccgtttcg attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg
    28081 atcatcgacg atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt
    28141 agattggata tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact
    28201 ggataaatca ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg
    28261 atggtgatca tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg
    28321 tctttttgct tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca
    28381 gcttcctcca ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt
    28441 tttcttttgc gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc
    28501 agtatctggc acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc
    28561 ttctttttct atttttgctt caagatcagt accctctttg cttacagatt gattttcagc
    28621 atcggattca ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat
    28681 ttcttttttc tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt
    28741 aaatacttca gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt
    28801 acctgtggcg ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac
    28861 tttgttttcc tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc
    28921 ttttgtatct tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt
    28981 atcgacgatt tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc
    29041 ttgattcttt cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt
    29101 ctctatatta gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc
    29161 gaataattcc attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc
    29221 gtttaattct gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc
    29281 tgtatttgtt tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc
    29341 gcagtctgga cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt
    29401 catcacttag cgatcggctc ctttactagg tttacgctac catttaatct atcatattct
    29461 atacgtcacg ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt
    29521 cctatagaaa aaaaagcttg agactgcgta gtctcaagct
//
LOCUS       DAYYAJ010000021        29531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000021 DAYYAJ010000000
VERSION     DAYYAJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.021"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..642)
                     /locus_tag="ACHATZ_000808"
     CDS             complement(<1..642)
                     /locus_tag="ACHATZ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403360.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYS"
     gene            844..1143
                     /locus_tag="ACHATZ_000809"
     CDS             844..1143
                     /locus_tag="ACHATZ_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403361.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1408..2283
                     /locus_tag="ACHATZ_000810"
     CDS             1408..2283
                     /locus_tag="ACHATZ_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403362.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            2280..2498
                     /locus_tag="ACHATZ_000811"
     CDS             2280..2498
                     /locus_tag="ACHATZ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403363.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2485..5112
                     /locus_tag="ACHATZ_000812"
     CDS             2485..5112
                     /locus_tag="ACHATZ_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8403364.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            5127..5345
                     /locus_tag="ACHATZ_000813"
     CDS             5127..5345
                     /locus_tag="ACHATZ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403365.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6864..7178
                     /locus_tag="ACHATZ_000814"
     CDS             6864..7178
                     /locus_tag="ACHATZ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403366.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(7231..8004)
                     /locus_tag="ACHATZ_000815"
     CDS             complement(7231..8004)
                     /locus_tag="ACHATZ_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403367.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            8109..8354
                     /locus_tag="ACHATZ_000816"
     CDS             8109..8354
                     /locus_tag="ACHATZ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8403368.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            8510..9361
                     /locus_tag="ACHATZ_000817"
     CDS             8510..9361
                     /locus_tag="ACHATZ_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8403369.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            9394..10071
                     /locus_tag="ACHATZ_000818"
     CDS             9394..10071
                     /locus_tag="ACHATZ_000818"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8403370.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            10559..11524
                     /locus_tag="ACHATZ_000819"
     CDS             10559..11524
                     /locus_tag="ACHATZ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8403371.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            11546..11758
                     /locus_tag="ACHATZ_000820"
     CDS             11546..11758
                     /locus_tag="ACHATZ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8403372.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11833..12309
                     /locus_tag="ACHATZ_000821"
     CDS             11833..12309
                     /locus_tag="ACHATZ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8403373.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            12306..14114
                     /locus_tag="ACHATZ_000822"
     CDS             12306..14114
                     /locus_tag="ACHATZ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8403374.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            14191..14406
                     /locus_tag="ACHATZ_000823"
     CDS             14191..14406
                     /locus_tag="ACHATZ_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8403375.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            14498..15361
                     /locus_tag="ACHATZ_000824"
     CDS             14498..15361
                     /locus_tag="ACHATZ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8403376.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            15398..15934
                     /locus_tag="ACHATZ_000825"
     CDS             15398..15934
                     /locus_tag="ACHATZ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8403377.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            15948..16370
                     /locus_tag="ACHATZ_000826"
     CDS             15948..16370
                     /locus_tag="ACHATZ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8403378.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            16300..18699
                     /locus_tag="ACHATZ_000827"
     CDS             16300..18699
                     /locus_tag="ACHATZ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8403379.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            18731..20722
                     /locus_tag="ACHATZ_000828"
     CDS             18731..20722
                     /locus_tag="ACHATZ_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8403380.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            20745..20996
                     /locus_tag="ACHATZ_000829"
     CDS             20745..20996
                     /locus_tag="ACHATZ_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8403381.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20986..22215
                     /locus_tag="ACHATZ_000830"
     CDS             20986..22215
                     /locus_tag="ACHATZ_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8403382.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            22212..24293
                     /locus_tag="ACHATZ_000831"
     CDS             22212..24293
                     /locus_tag="ACHATZ_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8403383.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            24442..28362
                     /locus_tag="ACHATZ_000832"
     CDS             24442..28362
                     /locus_tag="ACHATZ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8403384.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            28363..29307
                     /locus_tag="ACHATZ_000833"
     CDS             28363..29307
                     /locus_tag="ACHATZ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403385.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            29311..29514
                     /locus_tag="ACHATZ_000834"
     CDS             29311..29514
                     /locus_tag="ACHATZ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8403386.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
BASE COUNT         8615 a         6681 c         7331 g         6904 t
ORIGIN      
        1 tgaatacttt gaaatatctt tataggctaa gtccatttcg tctaatagct gttttaaaga
       61 atcaatcatt ctttcttttt caccgtattt gaaatcacca tttcttatct tgtcttgaag
      121 tttactgttg gctcttttga aaaatagctc tttagagata gagtgtcttt ggttagcgta
      181 ctcttcaatc agaggattga tattattagc aagtatctca tttagataaa tttcaaaatc
      241 ttttgagtaa tattttgtaa atatattttt tattatagga ttcaataact catagtcttt
      301 atctagcgaa aacgtatcac tatatctttc ttgaatgtct ttaaagactt tgtgaggtat
      361 ttctgggaag tccttaatat atagctcata ccctatatcg ataaggtatg aagttatata
      421 ttcttctaaa gttccccatt taatccagtc aattgttgaa ttagtgatag ttgataataa
      481 tttcagcttt tgtggatttg gtaaattggt cccacgttcc cagttggata tggtcgcacg
      541 tggtgaatta tctattttcc ttccaagttc ttccatagta aaaccacatg aaaggcgtaa
      601 ttgtttgatt cgttttcctg cttctgaagg atttggcttc atcttttttc tcctttttat
      661 ttttaaagat agtatatcat atttataaaa taaaagtatt taaaagtatt tactttttat
      721 ctggagtatg atactttatt cttataaata caaataaata cattttttta cgcaatttgt
      781 atttatttgt acttataaag gtagaggagt tatagaataa ttaattaaaa aggagaggtt
      841 tttgtggaag caataaaatt aatgatttca gaagaacagg aggaaatgtt tctaaagcag
      901 ttttatcaaa ttgctacaaa agctattgaa aaggctcaag aaaatgcggg gatgacaagg
      961 gaatatctca atcaaaaaga aatggcggaa tacttaaatg tgagtgaaaa ctttttaaaa
     1021 aatgctgtga gaaatgaggg attgcccgta gttgaattag gggcaagaaa attttattcg
     1081 aaaaaggcag ttaatgagtt tatgtttgct cgaatgaaaa gaggggaaaa tattggaaga
     1141 tgatgaagta gaaattataa aaaaatgctt taaaaaaaga aaaaaagtgt tcccaaaagt
     1201 ttgcagactg ggggaacact caataataaa gtgctgagaa aactttattt atagctatta
     1261 tatcaaaaaa gaaggtgaaa aggtgttttc ttctggaaat gttgtagttg acaaacttag
     1321 agaaataaat tttgacggaa atgtaatcca cacacatggt acgatcaatt aagaaaaaaa
     1381 acgaaaaaag gtgttgagaa gccctatttg aatgcaatag ttatcttagc agaaattacc
     1441 tattggtata gacctaaaaa aatttttaac gatgatggtc agctacttgg ttatcaaaaa
     1501 aagtttcttg aagatatttt gcaaaaatca tataagcaat taagcaaaaa aacaggatta
     1561 tcagaacgag ctataaaaga tgcaattgtg tacttagaga aaaaggggat tatcaaaagg
     1621 gtttttagaa atatttatta tgagaaaaga gcagttacat tatcaaatgt tatgtacatt
     1681 gttttaaatg tacataaatt gatagaaatt acttttccta ctttggattg cgaaaaaaag
     1741 gagtatatgc cgaaaattcg tcagaggggt atcgaaaaaa agagatatgt ctctttaaaa
     1801 aaaggtagag gtatgtacaa atttgtatat acgtatacaa agactacttc acagaacata
     1861 tcaaagacta ctctcgttat ggaagaagag gatattaata taaagaggat ttacagtaag
     1921 attagtgttc tttatgacgc aaataacgaa gatggtcgaa aattatttca agtgtatcac
     1981 tatttgagaa acgctaatgt agatacagaa gatatcagaa aaatcattga atatttatct
     2041 gaaaatactc agtatcttat tgctgagtgt attataaaac aacatgaagc atgctttttt
     2101 caaagaaaaa ctgaagaagg gttatacgat tatgtaacat attttttgaa tggacttcag
     2161 aaaagatata aaagtcaatt tttagaaaat gtagagccta tagatcagtt caaaaaagct
     2221 ttcgaaatca aagagttacc tgaaattact atgcataatt ggttgaaagg agaagaatca
     2281 tgattaaaaa tcgcttaata gatcaaatgt ggtgtcttga tcaaaataca gatactctcg
     2341 aattaatcga gagtatctgc ttcaacacag tagttctcga tttaagagct gaaaatgaca
     2401 attgtgtaac tcatatcata attaatcaaa aaatggctca gcagctctct gagagcctaa
     2461 gaaaatgggc tgaaggagaa aactatgaaa gcaattaaag aaaaaatcgt tagtagaaaa
     2521 ataaactggt tagcactcag tgtacttgtt ttaatgatta gcgttgtttt tacaagtaat
     2581 attgttgaag ctagcatttt caattggttt gatgacacaa aaagtcaaac agagtttttg
     2641 aataattctc tttatagacc ttacctaaaa aaatacaatg gagatttcct tagtcagttc
     2701 gggatttggc ttggatgggc tgtggtaaaa ggcatgttta ctgtgactga ttcgattcaa
     2761 aatatgattc cagatgtgtt ggatttgttc aatttcattg aaagtacagg gctaaataat
     2821 gtttatcaat cagtcataaa tacaattgtc gttggattaa tgatcctttc attgatgttt
     2881 gttggctaca agatgattac tggaaaagga acaatagatt taagatctgt tggaatgaat
     2941 atagtgatgt ctgtagcgtt aattctttta atgccaacaa tgatttctag cggtattcaa
     3001 tttgccaaaa tattttacaa tgatgctact acgattacaa attcagatga cggagtcgct
     3061 tggtcattga taaaacaagg agtaacagat ctggcatata ttaataaaac agatcaatac
     3121 tcaagcattg ataaaacgga ggatcgaaat aagttaacac gaaaaaattt tcaacagaca
     3181 gatttaacac aagtgttgac ggataaagtg attgataaat tggaaaaaga aaatccagct
     3241 gcggataatc ttcgctatga attagttgaa aattctaata atgaatttgt tgccacaaag
     3301 ttttctgata acttcttatc aacattttct gatagcttga aatcaggtta ttatcgatat
     3361 caagcaaatc tttgggggat ttcgattggg ttgacagcgt tggcaatcgc ctatgtgttc
     3421 agcgcgtttg tgatcattac agcaatatta gaacttgcat ttaaacgtgt gttaggtgtt
     3481 cttgtttttg cgacagatat tgaaacagga caacgttcaa aagttgtttt atccgatatt
     3541 ttacaatgtt acttaacagt tggatttcaa gggtttggac tatctatgtt tgctatgttt
     3601 atcaattttt taaatgctgg acaaggtatc tccacaaata tttttataaa aacaattgct
     3661 tatatctgtg ctgtgtttgt gctgataaaa ggcagtggaa cagtcatgag atactttggt
     3721 gttgatattg gactcaaaga aggctatggg caacttgctt ctgcttttgg tatgggcgca
     3781 atgctgttta gaaaaggatc aactggattt aatcgtgcta aaggaagcgg aaatggaaat
     3841 ggatcagaca gtggagaagg agaagatcga aaaccagaaa agaatttcgg cgaaactcta
     3901 tcaaaaaaag ctggcaaaac tggaagagct ttgggatatg cccatgaacg tggattatca
     3961 ggtttagctt cagatggagc tacaatggct agtgaaagag caataaaacc atttaagagt
     4021 atgcgtgata tggctaatga taccaagaat aagttcaaag aaggtttaga tgatggtaca
     4081 gtgtccgcta ttaataaaaa tagcaaacct atgttagcta aaaataaaga agatgaaact
     4141 ggaaaatatg ctgattccat gccaacacgt ctttcggata gaaaagatgg tgctaaggtt
     4201 gaaaatgccg atagaattat gtctagttct gaacgtatgc gtgaagcaat gaaaaataat
     4261 gctgaatcga ataataatcc tgtaagtgca attcagcaaa aagttcaaca agatattgaa
     4321 gaacgtaaaa atgctatgca cggtcaagct aagtctgctg aagaattaat taatcagaag
     4381 cgacaagaag ctaaatacac gcctgaagca atgaatcgtg aggaaatgtt aaggaaacgt
     4441 gtagaagggc gtacaggagc taatacggac gaaaaagaag cgttgaccaa agaaagaatc
     4501 caagaagcta aaaactcaaa taccggatta gaaaaattgg tcaaagaaaa tctacaaaat
     4561 tctacaagta gtcaaggtgg ccgatcagta gatgtccgag aaaatattca aggttcattg
     4621 aatggtcatg tcggacggaa tgttgatgta agagagaatt tacaaagctc ttcaaatggt
     4681 caaggtacta agacaattga tgttcgagaa aatgtacaaa attctacaag tagtcaaggg
     4741 tttaaatcaa tggatgtaag agagaatgta caaaattcta caagtagtca agggtttaaa
     4801 tcaatggatg taagagagaa tgtacaaagt tctacaagta gtcaaggatt caaaacagta
     4861 gatgttcggg agaacgttca aaaagatagt ggagaaatga aagaaaaaac acaaaaaatc
     4921 aatatcgttg aagacagaaa gtcagcgaaa aaatttgatt caaatcatga aacagtgatt
     4981 attgatagtg aaatccgtga aaatgataag ggatcaaaac caagacgaag atttacctat
     5041 gaagacaacg agttattcag agatacactt aatgatccta atccgctttt cgataatcta
     5101 ttaaaaaaat aaaaggggga attgtcatga aattaattct aaagattagt acgcatgatt
     5161 tcttagaact tgacgaatta tgtgaatcag taaagaacgc aacaaatgaa aaaatcacgg
     5221 tcactaatta tattgaactg atgattcaag aagaattaaa gatgaaacat gagataaaaa
     5281 acaacttgaa atattgttat ctagaaaaaa gtgaagagat aatccatgaa ttaaatggct
     5341 tttaaaaatt gtatttgaca tgaatttacg acaaggaatt agtggggatt aagcgattat
     5401 tctcttccac tttagagaaa taatgggtta aaaagccaga atacgtagag caatattgac
     5461 aacctataaa ggagctattg ttgaaaattg aagtaacaaa aagataaagg gaggtttaca
     5521 tctatcagat taaaagtgat agtacaccca tgaaacctgc aaaagagttg aacctaatgt
     5581 atatcggttt aactcttttt atttgcaaat taaaaaaata tcaccgaagt gacgaatgaa
     5641 actatgacca aattaagcaa gaaaatatat cttgcaaata aagtagtata aaaagatatt
     5701 gataaagcca atcaaaactt tgaatttcaa acgttagata atcaaacaga tacaaaaaat
     5761 gaaatcagtt ctacgcgtac gattattaaa aataataaat tttcactaga agaaaatgga
     5821 aataaaatca aataattagg aagaaaaaac aaagcaatta gaaaagcctg caagcaaatt
     5881 tgagtaatcg agatccaatt tttttaagga agtttaacaa aaatatgtaa ttctattatc
     5941 actattaaag agaaagttgg agcggaatat aaagaaaaga gaaaagtaat taaacgcaaa
     6001 aaaatgtaga acttaaaaaa tataaagagg aacaaccgtt aaaattagga atgatgttgt
     6061 gtaaacatta taattgctat gatataaatg aagtgttaaa gaaagcaaat ttaaaccaat
     6121 tttgtgaaca gaaaagcctt agtcactatg accaaaacta cggacgaaac tatgaccaaa
     6181 agtaggtact aaattatgac cgaaaatagg tccaaaatgg atttttttat gatatcaaaa
     6241 attatgaaat gattttgaaa gaaaaccagt tggcacagcc aacttagcca ccttcctgta
     6301 ggaaggtgct atcagttttc ccttatgctc ttttctttct tttttgtttc atatgtcaaa
     6361 tgccattttg aaatgtacca tcttaagaaa tttatcctta aaaacaattg cgttattgga
     6421 taaaaagatg ctacattatc ttaaaaaagg aatcaaatat gatgaattta gtggctagca
     6481 tatcgtgtga ttaagatact ttatagtgta agttgaagga gaaaaaatat ttaaaatata
     6541 cttcccattt cagtgggaag taaaattaat cttgttgttt ctactagtaa tgtatctttt
     6601 tggaaactat gtaacgatgt aaacaagatt tttttcagaa gaaaaaacga taaaagttgt
     6661 gataaacgat ttaaaaaata ggagaaaata tatcaagcgc atggtatgaa acgcacattt
     6721 aaaaagattc gactaactaa aaaaataagt ttacaaagca ttgttgtgta ttacaagtga
     6781 tctatacact gaaaaatgat agagaaaatc tgatacgtcc gcaacaatgg acaattgaaa
     6841 atgaaagtga ggaaattgga agaatggctg atgaaaataa agttgttccg cttgaaacac
     6901 tagtgataaa taaggaagag tttgagcaac tttttcttga tttttttgtt caatatgaca
     6961 ggatttatga ttttagttcg tcactagatt tatttgaatt agaggcagaa ataatagaaa
     7021 acagtcgaga ggagaatggt aaaatcatca tttcagtaga aaaattcaat gaattattct
     7081 cagaaacatt tgaaatagat acagattatt atcaaccaca agaagttttc caatcattta
     7141 gaaatcagat tattcgctca ggtaagaccg taaactgaaa aacaataaaa gaaacgttga
     7201 aagaataatt attccttcaa cgtttctttt ttagaatgtt ttaatgacca aagcagtttc
     7261 attgtcaggc ttaggtaatt catgaaatcc cgtatttata gtgctcaatt gatcaactaa
     7321 ccatgtttta ctttttgaat tgtagataaa tctaaaatta atatcgttat tatcgtgttg
     7381 aaggggcgtc tccaaataat ctgctgtata ttcatagtca acgtaacctg ctaatacaaa
     7441 agagagtatc cctttattta atgttagatt taatggataa tcatacgact ttattttttt
     7501 caacttcata ttgtacatat cttcagccat aaaagttgag ttcgtgattt catcttgggt
     7561 ctcattaaaa aagttatccg aataatcgct tatttttgaa aagtctttat tggcaccagc
     7621 ctcaaaaaat tgttcaactt tttcagtata aagttgtgct aacgctcctt tttcaatatt
     7681 aactttaata ttttcatgtc tatcaatttg tgtgctgttc agtaagggaa gatcagcttt
     7741 tagttttata tcgtcttgat ttctttcaat ttcaattggc aaaatctttt cagtagattc
     7801 agtttctgat aaaggaacat ctagtttttt atcatgataa taagcttcaa atccatctgg
     7861 aaccggaaaa aaatgtattg ggtaattctt ttctgaggtt gttgtactag tttccgtact
     7921 tgtagaactt gtgtcagaag tggtacaccc gcttatagtt ccgatgagta aagcgcaagt
     7981 tagaatgatt gcaaatttct tcaaaaaaat tcctccttaa aattgaatat ggactaatac
     8041 tagtcccatt gtagcatacc gaatctttaa gattaataag aaaaaggact aattttagtc
     8101 caaaaggtgt gctattcttg aaagaggaaa aaattattga taaagaaaat gtaatcggta
     8161 gcaatattag acgagtccga ttagaaaaag gaattggtca aaccgagctg attagagacc
     8221 tacagcttcg taaaattgct ataactaggg aaacgttggt taagattgaa ggtggccgcc
     8281 aacacattaa gttggatcaa ttgaaagcca tcaaagatat tctacaggtg tcttatgaag
     8341 atttgcttca atgactactt gtagtcattt cgtttgaaat tattatgtta cacccttatg
     8401 tgtcacgaag aaatactggt ataataatga atgggatatg taacgatagg gtatactgtt
     8461 atgttgccta ataaattgtg gatcaaaata caagaatgga gggatatcct tgtataaggc
     8521 atatgaattg gattttacaa atgctgactt aaatgcattc tcctcaagct cgcaatcata
     8581 tacacatgta gtaaatcaaa tagatcacaa ccaaaaatct attaacaaga gaatagaaaa
     8641 tctatttgaa agtgaaaatg atttagttga ttattattct aaggacagca aaattctaga
     8701 tgcagacgaa attattaaag attggtttcc aactataaaa gcagatattt ttatatctca
     8761 ctctcatgct gatgaaaaat tagctatacg ttttgcatca tggctatttg aaaattttgg
     8821 tcttactgca tttattgatt cttcggtttg gggatacagt tctgatttat taaaaaaaat
     8881 agaccaaaaa tattgctata aggaacaaac aaaaacatat gattatgata aaagaaatgt
     8941 aacaactagt catgttcata tgatgctttc tacagcttta aataatatga ttgatagtac
     9001 agaatgtctg ttttttctta atacacctaa ttcaattagt ttgagcaatg aaatcactaa
     9061 tgaacagaaa tttacgtatt cgccatggct atatagcgaa ctgacgacag cttcaatagt
     9121 ggaaaaaaag aatccacgat tggaaagtaa tcctcaaatg agcaccgagg atgttagaag
     9181 cattataaag cactattctt cgcaagaaaa tctgcaaata aaatattctt taaatttaag
     9241 tgaattaact tatttacgaa aagctgattt gaaaaaatgg gctttagatg ctgtggaatg
     9301 cactaaagga agtaatacat tagatatttt atataaaaac accattttca ctaatctcta
     9361 atcaatttac tgaatcgaaa ggaaccgggt agaatggata agaaagtttt aataaagagg
     9421 ttctctgaag ctattgaaaa aggaaatgca gcaatatttg caggtgcagg attatcgatg
     9481 tcacaaggat atgtaagttg gccggaacta cttaatgatc ctgcaacaga aatcgggttg
     9541 gattctaaaa aagaaacaga cttagttact ctggctcaat attataaaaa tgaaaatggt
     9601 ggatcaagag gaattttgaa tcagatactt atggataatt ttggtgaaga attagaaata
     9661 tctgaaaatc atcgaatact agcaagttta cctattgaaa cttattggac aactaattat
     9721 gatcatctaa tagaaaaatc aatccgagaa gcttataaaa accctcaggt taagaaaaat
     9781 tatactcagt tagcaacaac taatccaaat gtggatacaa ttgtttataa aatgcatggt
     9841 gatatagatg atgtcagttc gacagtaata acaagagatg attatgaaaa atatgatgat
     9901 tcgtacgcgt tatttaaaga aactttaaaa ggagatttgt taacaaaaac ttttctcttt
     9961 ttaggattta gttttacaga ccctaattta gaaagaattt tatcagatat aagatgggtt
    10021 ttgagagaaa accaaagacc tcattattgt ataatgagaa aaatattata gaaaaaattt
    10081 taggttatcg ctgaaagccc cggaaatacg ggcatttcgc aggatatgag aactcaattt
    10141 actaccaatt tactactttc ggattgagcc gcatatatgc tgaaccatac tgctaatacc
    10201 cgccacccca aatacggcac atcataaatt gaatacgaca ccgtaaacgc ggcgttcctc
    10261 tttcccgtca tgggagaaca ggacgccgct tttttatgcc caaatagaga aaggaggcga
    10321 cgccatgcta tttacttccg gccctgaccg ggcgtttgaa cggctgatgc aggaaaagcc
    10381 cggtttcgat cactacgacg gcggcggggc ggacgcgccg gaggattgcg gcacctgccg
    10441 attttaccgc ccggattgga aatatcaatt ctgccagttt gcagagtgtc cctatcagcc
    10501 gggcaagctg accgactatg cagcggtcaa attcacagtg aaaggagcga acaacgctat
    10561 ggcagttttc cgtgtggaaa agaacaaagg ctatacggtg atgtcaaatc accacctgcg
    10621 gaataagaac ctgaccttaa aggccaaggg cctgctgtcg cagatgttgt ccttgccgga
    10681 ggattgggac tataccctaa aaggactgtc ctatatcaac cgggaaagca tcgacgcgat
    10741 caggacagcg gtatgggagc ttgaaaaagc tggatatatc cggcgtgagc agggccgtga
    10801 cgcaaaaggc aaaatggccg atatggtcta taccatctac gaacagccgg tattggaaaa
    10861 cccggtattg gaaaatccaa catcggataa cccggtgttg gaaaatccga catcggataa
    10921 tccggcgtcg gaaaatccaa cgcaattaaa taaagaagta caaagaactg acttatcaaa
    10981 aaaagaaaaa ataattacag atggacaaag tacccattcc attcctatcc catccccgac
    11041 ccctgcccct atggagggcg aggcagcggc tacgccgccg gaacggaaaa ggacaggaaa
    11101 ggatgaggct gtacggattt atcgggaaat cattttggag aacatcgagt atgcctatct
    11161 cattcaggac aggagcattg accgtgaaca gcttgacgaa atcgtagacc tgatgctgga
    11221 aaccgtctgc accgcccgaa agacaatccg tgtcgccggg gacgactacc ctgccgagct
    11281 ggtgaaatcc aagtacatga agctgaccgg cgaacatatc cgctttgtcc ttgactgtct
    11341 gcgggagaac acaacgaaag tccgcaatat caaacaatac ctgcgggcta tgctgttcaa
    11401 cgcccccagc accatttcca actactacac ggcccttgtc gcgcacgaca tggcacagcc
    11461 cgattgggga aagcctaaat ccggcctgcc ggactattcc tgcggcgagg gcgaaagcct
    11521 gtaacgagaa aaggaggacg accctatgaa gcagggcgca ttgatttttg acgagcgaac
    11581 agaccgttac gacatccgct ttgatctggc cgactattac ggcggcctgc attgcgggga
    11641 gtgtttcgac gtgatgatcg gcggcaaatg gaggcccacc cgcattgaaa tggccgaaaa
    11701 ctggtatctt gtcggcatcc gggccgacga cctttccggc ctgcgggtgc ggatttgacc
    11761 tatatctacc tgcggcgtct ggtggttccg ccttagacgc cgcttttgac tttctgacaa
    11821 ggagggatgc gattgcagga agaagttact caaaagacca ttgccctgtc catgaaaacg
    11881 ggcaagctca cggctcaagt gctgcaagcg gcgctgaaaa agtacctgca acaccgggcc
    11941 aagggcaaaa acacgctgca ccacggccag cagagcctaa agcagcttaa aaagcatggg
    12001 gctgatctct ccaacattga gatcaccgag gccaacattg gagcgttcaa gccatgcgcc
    12061 aagaaatacg gcgtggattt taccttacga aaagataaga ccacccagcc gccccactac
    12121 gttgtgattt tcaaggccaa ggatgcagat aaccttgatc tggcgtttcg ggagtttacc
    12181 gccaagacgc tttccaagga gcagcggccc tctatccgca aggttttgtc cgctgtgaag
    12241 caaaaggcgg cggcccagac caagcagcgg gccaaggaga aaatcaagga aagggggctg
    12301 gaactgtgaa gcctgacgta aagaaattgc tgatcctgaa cctcccgtat ctgatttttg
    12361 tgtacctgtt tgcgaaatgc ggggaggcgt accggctggc cgctggtgct gacctgtcgg
    12421 ggaagctcct gcattttgcg gatggttttg cggcggcttt tgccaaccct ctgccaagcc
    12481 tgcacctgtt tgacctgtgt atcggtatcg ccggtgccgt gatcgtgcgg ctggtggtgt
    12541 actgcaagag caagaacgtg aagaaatacc gcaaaggcat ggagtatggc agcgcccgct
    12601 gggggacggc caaagacata gcgccttaca tcgaccccaa gtttgaaaat aatatcctgc
    12661 tcacccaaac ggaacggctt accatgaccg gcaggcccaa agaccccaag acagcgcgga
    12721 ataaaaacgt gctggtgatc ggtggcagcg gttcagggaa aacgagattt ttcgtgaaac
    12781 ccaacctgat gcaatgcttt ccgaccacgg actatccgac ttcgtttgtg gtgacagatc
    12841 cgaaaggcac tttggttctg gaaaccggca ggatgttcca gcaggcgggc taccgcatta
    12901 aaatcctgaa taccattaac ttttccaagt ccatgaagta caaccccttt gtctacatcc
    12961 attcggaaaa ggacgtgcta aagctggtaa ataccctgat ttccaacacc aagggcgagg
    13021 gggaaaagtc agcggaggat ttttgggtga agtcggaacg gttgttttat tcggccctga
    13081 tcggctatat ctggtacgag gccccagcgg aggaaatgaa cttctcaacg ctgctggaaa
    13141 tgataaacgc cagtgaagcc cgcgaggacg acccggagtt tcaaagcccc gtcgatctca
    13201 tgtttgagcg tttggagcag aaagaccccg accattttgc ggtgcgccag tacaagaaat
    13261 tcctgctgtc cgcaggcaag acccgttctt ccatcctgat ttcatgcggt gccagacttg
    13321 cgccctttga catccgggag gtacgcgagc tgatggagga cgacgaaatg gagctggaca
    13381 ccatcgggga cgaaaagacg gtgctgtatc tcattatgag cgatacggac acgaccttta
    13441 atttcattct ggctatgctc caaagccagc taatcaacct gctttgtgac cgggctgatg
    13501 atgtgtacgg cgggcggctg cctgtccatg tacgcctgat tttggatgaa tttgccaaca
    13561 ttggccaaat acccaatttc gataaactca ttgccaccat tcgaagccgt gaaatatcgg
    13621 cctctattat tttgcagagc cagtcgcagc taaaagcgat ttacaaggac gccgccgaga
    13681 tcatttcgga taactgcgat agtgtgcttt tccttagtgg gcgcggcaaa aatgccaagg
    13741 agatttccga tgcgctgggg aaagaaacga ttgacagttt caacaccagc gagaaccggg
    13801 gaagccaaac gtcccacgga ttgaactatc aaaaattagg aaaggagttg atgtcccaag
    13861 acgaaattgc cgtgatggac ggcggcaagt gtatcttgca gctacggggt gtgcgcccgt
    13921 ttttatcgga gaaattcgat attaccaagc atccccggta caaatacctt gccgacgccg
    13981 acaagaagaa tacctttgac gtggacagat ttttgactat cacgcgccgg aaacggcagc
    14041 aagtggtaac gcaggatgaa gtattcgacc tgtatgaaat cgacctgtcg gacgaggacg
    14101 ccgccgctga ataaacagcg gcttttttca ttccaacaac aaaatcaaaa aatcattatg
    14161 agccggaaac cggcagaaag aggtaaattt atggcatttt ttaattccgc agttactact
    14221 ttgcagacta tcgtgatcgg actgggcggc gctctgtgcg tatggggcgg catcaatctg
    14281 ttggagggat acgggcagga caatccgggg gcaaaatcgc agggtgtcaa gcagcttgtc
    14341 gctggcggcg gcgtggccct gatcggtgtc actctggttc ccctgctgtc cgggctgctg
    14401 ggctgatcct ggcctttgtc cctgtccaaa cccttaacta atcgcctccg cgtcctcccg
    14461 gccagaccgg gagggcgcgg gaaaggaggt gctacccgtg attagtgaaa tcattgaaga
    14521 atggataaaa ggcatcctca tggacggtat cacgggcaac ctgtccgggc tgttcgatac
    14581 ggtaaatgcc aaagtcggag aaatcgccgc cgacgtggga cgaaccccgc aggcatggaa
    14641 cagcggtatt ttcaatatgc tgcacagcct gtcggaaacc gtgatcgtcc ccatagccgg
    14701 ggccatactc gcccttgtca tgtgctatga gcttatccag atgataacgg aaaagaacaa
    14761 tatgcacgac tttgacagct ccatgttctt ccgctggata ttcaaatccg cgtttgcaat
    14821 cctgatcgta acgaacacat ggaacatcgt catgggcgta ttcgacgcca cccaaagcgt
    14881 cgtcaatcaa agcgccggtg tgattatcgg gcagacatcc attgatttta acaccctgat
    14941 accagacctt gaaagccaat tggaggctat gagcgtgggc gctcttttgg ggctgtggtt
    15001 ccaaaccctt gttgtgggcc tcaccatgaa tatcctttcc atctgcattt tccttgtgac
    15061 ctatggccgc atggtggaaa tctatgttgt cacgtcgctg gggcctatcc cccttgccac
    15121 aatgggcaac gcggaatggc gcggcatggg ccaagggtat ctgaaatccc tgctggcgct
    15181 gggcttccaa gcctttttaa tcatggttgt ggtgggtatc tacgcggtgc tgatacagaa
    15241 tatcggttcc gcttccgaca tatccggcgc aatctggggc tgtatgggct atacggtgct
    15301 gctgtgcttc tgcctgttca agaccggcag tatatcaaag gccgtattcg gggcgcattg
    15361 atcccaaaat ccaagcatac acaggaggaa cttgtttatt tcaacgtaca acatcattta
    15421 cgctgacccg ccgtggcggt atgactgcaa aaacgtacag ggcgcagcgg aaaatcacta
    15481 ttccaccatg agcattgacg agctgtgcgc cctgccggtg gaacggctgg cgtccaaaga
    15541 ctgcctgctg tttttgtggg ccacattccc catgctgccg gaagctctgc ggttaatcaa
    15601 agcgtggggc ttttcattca agaccgtggc ttttgtatgg ctgaaacaga accgtaaaag
    15661 ccttacatgg ttttacgggc tgggccgctg gacaagggga aacgcggaaa tctgcctgct
    15721 ggcaacacac ggcaagccga agcgtcgttc tgcctctgtc catcagttta ttatcagccc
    15781 gattgaacaa cacagcaaaa agccggatgt gacccgtgaa aaaatcgtag agctggcggg
    15841 cgatttgccc cgcgtggagc tgtttgccag acagaaaacc cccggctggg acgtgtgggg
    15901 gaacgaggtg gaatgtgacc ttatcctgac gtgaaaggag gtgccaaatg gcttatgtaa
    15961 ccgtccccaa ggacttaacc catgtgaaaa gcaaggttgt gtttgggctt accaaacggc
    16021 aactgatctg tttttccggg gcgctggtga tcggcgtccc cttatatttt ctcacccgtg
    16081 actatcttac caacagcgcg gcggccctgc tcatggtatt tgccatgctg ccgggctttt
    16141 tgcttgccat gtacgagcgt cacggccagc cccttgaagt ggtgattatg cagatcatcc
    16201 agtgctgctt catccagcca aaggagcgtc cctatcagac cgacaacacc tacaccgccc
    16261 ttgtgcggca ataccagatg gaacaggagg taaaggccat tgtccaaaaa ggaaatccca
    16321 agagaaaccg aaaaaacaaa gctgacccgc gcccagcgaa aagaaattga cgcggtaatc
    16381 cgtaaatata agggcgacgg caggccccac acggcgcagc agagcatccc ctacgaggtc
    16441 atgtacccgg acggcgtgtg ccgggtatcg cccggcgtct tttccaagtg cattgagttt
    16501 gcggacatca gctatcagct tgcccagccg gacacgcaga cggctatctt tgaaaaactg
    16561 tgcgacctgt ataactatgt ggacgcctcc attcatatcc agttttcttt cctcaatcgg
    16621 aaagttgacc cggtgcagta cgccaagagc tttgagatcg cgccgcaggg ggacgacttc
    16681 gacgacatcc gggccgagta caccggcatt ctgcaaaagc agcttgcaaa cggcaacaac
    16741 ggcatggtaa agaccaaata cctgacattc accatcgagg ccgaaagcgt gaaagcggcg
    16801 cgagccagat tgaagcgcat cggctttgac ctgttaggct atttcaagag catgggggcc
    16861 gtagcccatg tgatggacgg ctgggaacgg ctgaacctgc tgcatggcgt gtaccacccg
    16921 gacggcgaga tattcaattt tgactggaag tggctggccc cgtccggcct ctccaccaag
    16981 gattttattg ccccgtcctc cctgtgcttc ggcaatgcaa agacattcgg catgggcggc
    17041 aaatacggcg cggtaagttt cctgcaaatc ctgtccccgg agctgtcgga cgatatgctg
    17101 gccgacttcc tcaatacgga aagcggtgtt ttagtcaatc tccatgtgca ggccattgaa
    17161 cagaccaagg cgatcaagac catcaagcgc aaaattaccg accttgacgc tatgaagatt
    17221 gcggagcaga aaaaggccgt ccgcagcggt tacgatatgg acattcttcc cagcgacctt
    17281 gccacctacg gcgaggacgc caagaagctg ctgacaaagt tgcagacccg caatgaacgg
    17341 ctgttccagt tgacgttcct tgtcctcaac gtggccgaca ccaagcaaaa gctgaataac
    17401 gacgtgttcc aagccgcagg cgtggcccaa aagcataact gtccccttgt ccggctggac
    17461 taccagcagg aacagggcct tgcaagcagc ctgccgctgg gcgtcaatca gatcaagata
    17521 cagcgcagcc ttacgacttc cagcgtggcc gtgttcgtcc cctttgtgac gcaggagctt
    17581 tttcagggcg gcgcggccat gtattacggc atcaacgcca agtcccgcaa catgattatg
    17641 ctggacagga agcaggcccg ttgccccaat gccttgaagc tcggcacccc cggcagcggc
    17701 aaatccatga gctgtaaatc cgagatcgtc agtgtgttct taacgacgcc tgacgatatt
    17761 tttatttcag acccggaggc cgaatattac ccccttgtca aacggctcca cggacaggta
    17821 atccggctgt cgcctaccag caaggacttt gtgaacccgc tggacatcaa cctgaattat
    17881 tcggaggacg ataacccgct ggccctgaaa tccgactttg tgctgtcgtt ctgtgagctt
    17941 gtcatgggcg gcaagaacgg gctggaagcc attgaaaaga ccgtgataga ccgggctgtg
    18001 cgcgtgatct accggcccta tctggcagac ccccgcccgg agaatatgcc gatcctgtcc
    18061 gacctccaca aagcgttgtt agatcagcat gtcccggagg ctgaccgggt ggcgcaggcc
    18121 cttgacctgt atgtgtccgg ctccctgaac gtatttaacc accgtaccaa cgtggacatc
    18181 ggcaaccggc ttgtgtcctt tgacattaag gagctgggca agcagctcaa aaagctgggg
    18241 atgctcattg tccaagacca gatatggggg cgcgtcacgg ccaaccgaag ccaaggaaag
    18301 gccacatggt attttgccga cgagttccat ttgcttttga aagaggaaca gacggcggcc
    18361 tattctgcgg agatttggaa gcggtttagg aaatggggcg gcattccaac aggtgccacc
    18421 cagaacgtca aagacctgct ttcctccccg gaaattgaaa atatccttga aaacagcgac
    18481 ttcattacgc tgctcaatca ggcgtccggg gatagaaaaa tccttgcgga acgcttgaat
    18541 ttgtccaccg aacagcagaa gtacatcgac aattccgaac ccggcgaggg ccttttgatc
    18601 tttgaaaacg tggtgctgcc ctttaccaac cccatcccgc acaacaccca gctttacaag
    18661 attatgacga cccgcttaaa cgaggtggcg ggagtatgac ccggcgacgc cacaaaggag
    18721 gtgaaccacc atgaaagagc tgaaagccaa ggcgaaagtg acgcaggcca tgacccgcga
    18781 cgggctgacg ctggaaaatc aggccaccgg cgaggtggag aacatttcca gccgggaggc
    18841 cgaacaggat ttgtccgcgc cggggccggg cggcaccgcc gataagctgc tggaacgggc
    18901 cgacgccatc catgacggcc ataaggccaa gaaagccgcg aagcaggccg gggctgtggc
    18961 cgccgagggg gacgctgccc tgcaccggcc atccgcccgg ctccaactgt cggaggaaga
    19021 acgggccgac ccggatttac agccctatat ccgaaaatcg gacaaacggg ccgataagct
    19081 ggataaggcc cgcgccgcca tccccaagaa gcgcgtcccg gtcaaggaaa ccgtctttga
    19141 cgaggcaagc ggcaaagcaa agaccgtcct gcactttgac aaacaggaca aggggcctcc
    19201 ctccctgaaa cccaatccgg caggccgtcc tgtttccgag gtttttctat tcggccacgg
    19261 gaaaatccat gaagtcgagc atgaaaacgt gggtgtggag ggcggccata agggggagga
    19321 acttgccgag cggcaggccg gaaaagccat ccgcagcacc atccggcacc acaggctgaa
    19381 accctactgg gacgcggaaa aggccgagcg caggctattg tctgccaacg cggagtattt
    19441 ctaccagaaa tctttgcggg ataacccgga gatcgcacag gcggccagca accccatttc
    19501 ccggttctgg cagaaacagc agatcaagcg ccagtacgcc aaggccgccc ggacagcggg
    19561 ccagaccgca ggcgcggcgg cacagggcgc ggccaagaca gccggggcct ccacgaaagc
    19621 ggccaaggcc gcagccaaag aaagtgaacg ggcggcctcc tttgtggccc gccactggaa
    19681 aggggcgctg gccgtccttg tgttggggct tttgatgctg cttatcatcg gcggcttaca
    19741 gtcctgcacc gccatgttcg gcagcgccgg aagcggcatc acggcgtcct cttacttttc
    19801 cgaggatagc gatatgttgg ccgccgagga cgcctacgcg gagctggaag ccgagctgca
    19861 agactatctg gacaactacg aggccaccca tgactatgac gaataccatt ttgaccttga
    19921 cgagatcagc catgacccct atgtgctgat ttccattctg tcggcgctcc atgacgggat
    19981 atttaccatc ggggaggtac agggtgattt gtccatgctc tttgacaagc aatatatcct
    20041 gacagaaaac gtcgtggtag aaacccgtta ccgcaccgaa accgatacat ggacggacgc
    20101 ggagggcaac acccatacag acacctacca agtgccgtat gactattaca tctgctacgt
    20161 cacgctggaa aacttcgacc tgtcccatgt gccagtctat atcatggacg aggaaacgct
    20221 aagtttctat tccctgtata tgtccacgct aggaaaccgg cctgatctgt tttccggcag
    20281 ccagtacccc aacgcctcca ccctgacgcc gccgacctac tacgacgtac ccccggaggc
    20341 tttggaggat gccacctttg ccgccatgct caaagaggcg gaaaaatata tcggttatcc
    20401 ctatgtgtgg ggcggcagct ctcccagcac cagctttgat tgcagcggct tcatttcgtg
    20461 ggtaatcaat catagtggat ggaacgtggg acggctgggg gcgcagggcc tatataacat
    20521 ctgcacccct gtttccagtt cgcaggccaa gcccggcgac cttgtatttt tcaagggaac
    20581 ctacgacacc cccggcgtgt cccacgtcgg cctgtatgtg ggcaacaatg tgatgctgca
    20641 ttgcgggaac ccgatttcct acacgaacct tacttcaaac tactggcaat cccattttta
    20701 cagttacggg cgtttacctt aacgccagaa aggagctttc aattatggca accaaacttg
    20761 accgcatcga aaaggacatc cagaaaacca agtctaagat cgcggagttt cagaaacagc
    20821 ttcgggagct ggaaacgcag aaaaccgaac aggagaattt gcagatcatc cagcttgtgc
    20881 gcggcatgaa catgaagccg gaggaatttg ccgcgttctt acgcagcggc gcaatgcagg
    20941 ccgcccctgc cgcaacccct taccatgaac aggaggacaa tgcccatgaa gaataagacc
    21001 agacggattt cccgtattct ccccgcactt ctggccgtga tgctctgcgt cacggccttt
    21061 cccgttaccg cccttgcagg cggcaatgac ccggcaccgg ccccgctgcc ggaggctaca
    21121 accgaacccg ctacgggcgg tttggagcct gaaacagacg aaaccggcac cgccacggac
    21181 gggacagaaa ccgaggccga aaaagatacc ggccctctga ccggcaagga catcaccgac
    21241 ctgttttccg cgctgttcgg ttccaaggtg agcattgccg ccacggacga cggcatccag
    21301 atcaccaccg gcaccgacaa aaaagaacct gaacagaccg gcaccgtcac caccaacggc
    21361 ggcaggctga atgtccgcac cggcgcgggg cttgacaaga ccgcgttcac ccagcttccc
    21421 aatgggacga cggttgaggt agtcggcaca gacggcgact ggattaaaat tctcttgccg
    21481 gagcgcatcg gctatgtcca ttccgactat atgaccgtca gcgaaaagga agtagcggct
    21541 accggcgagg gcggtttttc cctctccatt gacccggagg aaattgcttc tctgctggaa
    21601 ctgttcaatg gcggcggggc cagcgccgcc ctgtccccgg acgggaacct gtcactgatt
    21661 gacgacatcg gcagcgccgc gaaaaatggc aagcagttta tcaccgtgga aaccaaaaac
    21721 ggcaatgtgt tttatctgat tattgaccgg gacgacgagg gaaaagaaac tgtccatttc
    21781 ttaaatcagg tggatgaagc cgatctgctg acgctgatgg aggacgggga aaccgtcacc
    21841 cccgctgctg tctgctcctg taccacgaaa tgcaaggccg gggctgtgaa cacgaattgc
    21901 ccggtatgta aaaccaacct gaccgagtgc agcggcccgg agccgcagga accccagccg
    21961 gaggaacccg aagccccgga ggaagaaccc aagggcggcg cgggcggcct gatcgtgttc
    22021 ctgctggtgg cgctggcggg cggcggtgcg gccctttact atttcaagtt caaaaagccc
    22081 aaggctgaaa ccaccggcag cgacgatttg ggcgagtatg attttgggga ggacgaggat
    22141 ttggaggacg aggaacccga aaccgagttt gacccggaag ctgattttgt gagtgaacag
    22201 gaggacgagg aatgacatat aaacttgtaa tcgcggaaaa acccagcgtg gcccgcagca
    22261 ttgcgggcgt gatcggggcc acggagaaac acgacggcta tttggagggg aacggctatc
    22321 tggtgagctg gtgcatcgga catctggtgt cctttgccga cgccggacgc tatgacgagc
    22381 gtttcaaaaa atggcgctat gaggatttgc ccattctccc ggaaacatgg cagtacatcg
    22441 tccccgacga caagaaacag cagtttgacg ttctctgttc gctgatgcag cgccccgacg
    22501 tgaccggcct tgtgtgcgcc accgacgccg ggcgcgaggg ggaattgatt ttccgtttcg
    22561 tctaccagat ggcgggatgc caaaagccct ttgagcgcct ttggatttcc tctatggagg
    22621 acagtgctat taaggacggc tttgcccacc tgaaaccggg cggcgactat gatccgctgt
    22681 atcaatcggc cctgtgccgg gccaaggccg actggctgat cggcattaac gccacccggc
    22741 ttttttccgt cctgtaccac aagaacctga ctgtgggccg cgtccagacg ccgaccttaa
    22801 aaatgctggt ggacagggac gccaagatta ccgatttcaa gaaagagaaa tatcacatcg
    22861 tccatatcac ggcgggcggc gcggacgcgg taagcagccg cttttccgac gctgccgagg
    22921 caaacaccgt caaggcggct tgtgcaaaag cgcaggccgt ttgcgtttcc gtgacgcggg
    22981 agaaaaagac ggaacagccg cccaagctct atgaccttac caccttgcag cgggaggcta
    23041 accggctttt cggctatacc gccaagcaga cccttgacta tgcccaggca ctgtatctaa
    23101 agaagctgct gacctatccg agaacggata gtcaatatct taccgacgat atgctgcccg
    23161 ccgtggaaag ccttgtgtcc ggcctgtggg agctggtgcc gtttgccaag ggcctgaacc
    23221 tttccccgca gtttgaccgc atcctgaaca gcaaaaaggt gtccgaccac cacgccatta
    23281 tccccaccgc tgaatttgtg aagcagggct ttggcactct ggccgagagt gaaaaaaagc
    23341 tgctgtccct gatctgctgc aagctcttat gtgccgtggc cgggccgcat atctatgagg
    23401 ccgtcaccgc cacatttacc tgtgccgggc aggagtttac cgccaagggc aagaccgtcc
    23461 tttcccccgg ctggaaagag attgaacgcc ggttccgttc ttccttaaag accgacgccg
    23521 acgaggacgc cgaggcggtg cgggagctgc cggagctttg cgagggccag acctttgcag
    23581 acgtggccgc ctctgtcacc gagcatttca ccacgccgcc caagccctac accgaagata
    23641 cgcttttgtc cgctatggaa cgggccgggg ccgaggatat gccggaggat gccgagcgca
    23701 agggattagg cacaccggcc acccgcgccg ccattctgga aaagctggtg cagatgggct
    23761 ttgtccagag aaaaggcaag cagctcattc ccaccaagga cggcatcaat ctggctgtgg
    23821 tgctgccgga ggcgcttact tctccccagc ttactgccga atgggaaaac cggctgaccg
    23881 agatcgccaa ggggcaggcc gatcctgacg aatttatgga cggcatcgag acgcaggcgc
    23941 gggagctggt gcagacctat tcctgtatca gtgaggaaaa gcagaaattg tttcaggccg
    24001 agcgtgttcc tatcgggacg tgtccccgct gtggtgaggc tgtctatgag ggcaagaaaa
    24061 actactactg cggaaaccgg gcctgccagt tcgtcatgtg gaagaatgac cgattttttg
    24121 aggaacgcaa gaaagcgttc accccgaaga tcgccgctgc gctgctcaaa accggcaaag
    24181 tcaaggtaaa gggcctctat tccgtaaaga ccggcaaaac ctacgacggg acggtgcttt
    24241 tggctgatac cgggggcaag tacgtcaact accgcattga acacaggagc taaaggacag
    24301 taacacagca agcataggga gtgtggctac actcccggta aaatcccccg gccgcgtccc
    24361 tgccgggccg gggatttttg cttgttgggg aagtccccaa ccccctattt tttgaaacag
    24421 aacagagaaa ggagtgaccg cgtgaacgaa gtattttcta tccaagtcca taaccggcag
    24481 tttttggaac agggcaaaca aggcgtctgg ctggatttgc ccaccacaac cgaaacgctg
    24541 caagccgccc tgcgggatgt tggcattact gccgacaatc cgcaggattt ttttatcaac
    24601 gggtattcct gcccggagga cagacacctt gccattccct atgacatggt gctggccgcc
    24661 gacgtggacg agctgaattt ccttgccgcc cggctgggcc agcttgacgc cgccgagatc
    24721 gcagagctga acgccgccct gcaaaatccc cgtggcggct ttgagaatat cggccagatc
    24781 atcgactaca ccgaaaatgt ggattactat gtgcatcttc ccgacgttag gggggatgga
    24841 cagttgggcg actactacct gaaccgttcc ggcatggtgg atatgccgga ggaatggaaa
    24901 gccgggattg atacggcgca gtttggacgc cacattgccc agcaggaaca aggcgttttt
    24961 acccagtacg gctatctggt gagaagcggc gacgaatggc agcgcgtcca tgagggccag
    25021 ccggtgccgg aggaataccg ggtgatgcac tacccccagc cggaagctga ccgggaggaa
    25081 ccgcaggccc agcagccgga ggcatccgaa agccccgaca aagcgccgcc cccggttatc
    25141 ccgatcatgc tggacggcat gaacagcaaa gaacgcatga aagtgattac tgaccgtctg
    25201 gaaaccggcg tacaggagct ttttgaaagt agccgctata tggcttactt atccgctatg
    25261 tccaagatgc acgattacag tctgaacaat acgctgctga tcgccatgca gggcggttcg
    25321 attgtggcgg gctataacag gtggcaggat gaatttgacc gccatgtgaa gcggggcgag
    25381 aaaggcatta agatactggc cccggccccg ttcaaggtta agcagcagat ggagaaaacc
    25441 gacccggcca cccagcagcc cgtgatcggc gcggacggca agcccgtgac cgaagaacag
    25501 gaagtgacgg ttccggcctt taaggtggtg acagtctttg acatcagcca gaccgagggc
    25561 aaggattttc ccatgcccta tatcagtgaa cttaccggcg acgtgcagca gtatcaggat
    25621 ttctttgccg cgctggaacg gacatcaccc tatcccataa gcattgagcc gatttcagac
    25681 ggggccaagg gccgctgctt ctacgcaaaa cagcgcattg ccatcaacga gggcatgagc
    25741 gaattgcaga ccctaaagac cgccatccat gagattgccc atgccaagct ccacgccatt
    25801 gaccgggagg cccccgccga ggaacaggcg gcccggccag accgcagcac ccgcgaggta
    25861 caggccgaaa gcgtggccta taccgtctgc cagtattacg ggctggacac gtcggactat
    25921 tcttttggct atgtggccga ttggagcagc ggcagggagt tatccgagct gaaagcctcc
    25981 cttgaaacca tccgggccac ggccagcgaa ttgataaccg agatcgacgg ccattttgcc
    26041 cagcttcagc aggagcgcga ggcccggcag gaacaggagg ccgtcacggt tgacgttacc
    26101 ccggaggcag cggccccggc gcaggccgag gaagaacagg ccccggccct tgatccggca
    26161 gccgagccgc ttgtgactgt gctatggagc gaaagccccc agctaaagga cggccagcaa
    26221 atgccgttgc atgaggccga cgcgctgttt gcccgtctgg atgcagcata tccggcagag
    26281 gcaggctatg acaaaaccgc gttccgcatt gactgtatct ttcagggcga gccggacagc
    26341 tacgaggggc ggcaggattt tggagatagg gacggttccc tgatcgaaca tatccagagc
    26401 taccaccaat tttacgcgca ggacgaaaac tggaaaaagc atgtaatcgc tacaaccggc
    26461 atagagggct gggagcagga caaggcccat cgggatatgc tgttaggcga gtttgtcccc
    26521 tatatgaagc tgcattgcag ccttgcccaa atggaacaga cggcgcaggg ccgcttacag
    26581 tccggggaaa ccctgacgcc ggaggaaacg gcctatttgg gggcgcttct ctcttatgtg
    26641 caggagtgcc gccccatgct caatcagggc cagtatcagt tgccggagcc gccgaaactg
    26701 gctgacttcg accagagctt gcaggactac aaagcacagg tacaggccga gatcgcgcag
    26761 gaggccgccg cagccggtat gaccgtggag gaatacgcgg cggctggcta tgaagcaccc
    26821 caacaggaca ccttttccat ctaccagctt cgaggcgggg aggaaacccg cgactatcgg
    26881 tttgagccat acgagcgttt acaggccgca gggctggccg tggacagtgc caactatgac
    26941 cttgtttata cggcaccgct ggataaaagc acgtctttgg aggatattta ccgcacattc
    27001 aacatcgacc acccggcaga ttttagggga cacagccttt ccgtttccga cgtggtggtg
    27061 ctgcaccaga atggacagga caccgcccac tatgtagaca gcttcggcta tatggaagtg
    27121 ccggagtttt tgcgggaaaa tcccctgcgt accgccgaac tgtctacgga gcagaacgaa
    27181 aacatgattg acggcattat caacaatacg ccgcccgaac cgcagccgga ggaacccgaa
    27241 cagctatctt ttgaggatgt ggcagttgct tccggccaga cacaggagga acccccggag
    27301 gctgcgcccc cgcaaacggc tgtccgctat tatcccatca atgagggagc cgcccgccgc
    27361 gccaaggaag ccatgagctt ttctgactac cggcccggca gcgccacggc ggaataccgg
    27421 cactatgtcg atcaggccgt ggaaattgcg gagcgccaga aaaagcgtgt ggagccagag
    27481 tatcacgaaa agatagacag cctccttgac acctacgccc gcaagctggc cgagaacatg
    27541 aataagggct atgagattac cgcccgcgtc ccctctgtcc tgattgccgg gccgtccaat
    27601 ttcccggtac gggcgaaaga aaagcagaac gccgccagtg accgcaacat ggaggaattt
    27661 cagtacattc agggcttgct tgacaaaatc cgcagcaccg gcatgggtgg tatcagcgcc
    27721 gacgatccgc aggccgtttc caagctggaa aagaaactgg aaaagctgga agcgtcgcag
    27781 gagttgatga aggccgtcaa tgcctactac cgcaagcatg ggacgctgga cggatgcccc
    27841 catctgatcg aaagaggcat tgaaaacctg aaagcggata tggcgtcggg ctggcactat
    27901 gagaaaaaac cgttccagtc ctggcagctc tccaacaata acgccgagat acgccggtta
    27961 aagggccgca ttgaggaatt gacccgccaa aaggaggcgg cctatgtggg atgggagttt
    28021 gacggcggca ctgtggagat caaccgggtg gccaaccgcc tgcaaatctt ctttgagggg
    28081 aagccggatg ctgctgtacg cgacgagctg aaaagcaacg gtttccgctg gtcgcccaag
    28141 gccggggcat ggcagcggca gttaaacgac accacaatcc gcgtcgctga ccgtatcaag
    28201 tgcatccagc ccctttccgg cgaaaagccc agcgccttac agcttgcggc ccgcagggag
    28261 aaagaaaagc cctctatccg ggcgcagctc aacgcggcca agaccgaaca ggccaagcag
    28321 ccaccggcgc gggaaaaatc aaagggattg gaggtagagt aaatgcagaa gtttgaaaat
    28381 gtggacgttc tggccgcgct ggaacagatc atgcggcaga acaccgcgtt ttatcaaagt
    28441 gactttggca ttgacaaaga cattatcagg cgggatgccg ccagcgatca ggccgcagac
    28501 aagaccctgc tctggatgtc ccggccctcc gggacgtact gtttccggga acgtgatgtt
    28561 ttcctgaaag acacccgaca gtacaacaca tggaaatttt acggggagca gacccgtgac
    28621 aagatactgg cctacgcggt ggagctgacc ggcacacagg gcggcactat ccggggcaat
    28681 ctctatgagc tggactatcc gcagcatttc cggcacgtca ttgaaaccgc ccagccggtg
    28741 agtgtgaacc ggctctttta tgagcatggc acccgtgaca tcccggagag ccagcgtttt
    28801 gacggaagcc ctgaccgggt attgggaaac tttttgtact atgaggccca gccccatgac
    28861 ccggctgtgc tgcaggaggc gctacggacg gagcagcaca gccgggggcg ggccgtcccc
    28921 ggcgatttca aagtccacgt caccgctttg catgacagcc ggattgaaac cgaggcccgg
    28981 cgtattgtgg acagcctgaa agatctgtcc gcgcctaaca gccccaataa aacgcatttc
    29041 atggtggaga tctccccgca ctttgcggcg attgcttcca ccaaggataa tgaccggctc
    29101 tttgccatgc tgccctataa atccctgtgt tttaccggga tgaaaggcag gcatggcgtg
    29161 tatgccgtga ttaaccggga cgagcgccgg gatgtgagta ttcgcaggcc ccggccctct
    29221 atccgcaagc agctatccga cagcaaacag gccgccagcc caaaaaaggc ggcggccaga
    29281 accaaaaaga atgaattgga ggtataagga atgatgcact ttaccgtgga ggaagaaaac
    29341 ctgatctgta tgtaccacaa tgcagaccgg cgcagaacga taaacaggct caccgccgtc
    29401 atgccggtta tggatgggga tatgcgggcg caggcccagc agaccctttc caagctggaa
    29461 cgcatgacgg atgccgacta tgacggccag aagttccact tcacagacga ataacgaaca
    29521 gcagacgccg g
//
LOCUS       DAYYAJ010000022        29099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000022 DAYYAJ010000000
VERSION     DAYYAJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.022"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHATZ_000835"
     CDS             complement(<1..1031)
                     /locus_tag="ACHATZ_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8403387.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHATZ_000836"
     CDS             complement(1113..1802)
                     /locus_tag="ACHATZ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8403388.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHATZ_000837"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHATZ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8403389.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHATZ_000838"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHATZ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8403390.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHATZ_000839"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHATZ_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8403391.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHATZ_000840"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHATZ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8403392.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHATZ_000841"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHATZ_000841"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8403393.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHATZ_000842"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHATZ_000842"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8403394.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHATZ_000843"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHATZ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHATZ_000844"
     CDS             complement(7636..8523)
                     /locus_tag="ACHATZ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8403395.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHATZ_000845"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHATZ_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8403396.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHATZ_000846"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHATZ_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8403397.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHATZ_000847"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHATZ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8403398.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHATZ_000848"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHATZ_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8403399.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHATZ_000849"
     CDS             complement(10454..10957)
                     /locus_tag="ACHATZ_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8403400.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHATZ_000850"
     CDS             11554..12519
                     /locus_tag="ACHATZ_000850"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8403401.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHATZ_000851"
     CDS             complement(12618..13790)
                     /locus_tag="ACHATZ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403402.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHATZ_000852"
     CDS             complement(13777..14922)
                     /locus_tag="ACHATZ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403403.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHATZ_000853"
     CDS             complement(14936..15868)
                     /locus_tag="ACHATZ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403404.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHATZ_000854"
     CDS             15985..17064
                     /locus_tag="ACHATZ_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8403405.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHATZ_000855"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHATZ_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHATZ_000856"
     CDS             complement(17789..18016)
                     /locus_tag="ACHATZ_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8403406.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHATZ_000857"
     CDS             complement(18119..20707)
                     /locus_tag="ACHATZ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8403407.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHATZ_000858"
     CDS             complement(20727..21386)
                     /locus_tag="ACHATZ_000858"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8403408.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHATZ_000859"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHATZ_000859"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8403409.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHATZ_000860"
     CDS             complement(22159..23025)
                     /locus_tag="ACHATZ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8403410.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHATZ_000861"
     CDS             23134..23775
                     /locus_tag="ACHATZ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8403411.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHATZ_000862"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHATZ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8403412.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHATZ_000863"
     CDS             complement(25216..26115)
                     /locus_tag="ACHATZ_000863"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8403413.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHATZ_000864"
     CDS             complement(26117..26914)
                     /locus_tag="ACHATZ_000864"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8403414.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHATZ_000865"
     CDS             complement(26892..27596)
                     /locus_tag="ACHATZ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8403415.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHATZ_000866"
     CDS             27727..28302
                     /locus_tag="ACHATZ_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8403416.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHATZ_000867"
     CDS             28420..29097
                     /locus_tag="ACHATZ_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8403417.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaa
//
LOCUS       DAYYAJ010000023        28574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000023 DAYYAJ010000000
VERSION     DAYYAJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.023"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1372
                     /locus_tag="ACHATZ_000868"
     CDS             101..1372
                     /locus_tag="ACHATZ_000868"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8403418.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            1369..2664
                     /gene="rho"
                     /locus_tag="ACHATZ_000869"
     CDS             1369..2664
                     /gene="rho"
                     /locus_tag="ACHATZ_000869"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8403419.1"
                     /translation="MSDYLRMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            2763..3026
                     /locus_tag="ACHATZ_000870"
     CDS             2763..3026
                     /locus_tag="ACHATZ_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8403420.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            3471..3920
                     /locus_tag="ACHATZ_000871"
     CDS             3471..3920
                     /locus_tag="ACHATZ_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8403421.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            3977..5395
                     /locus_tag="ACHATZ_000872"
     CDS             3977..5395
                     /locus_tag="ACHATZ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8403422.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            5395..6255
                     /locus_tag="ACHATZ_000873"
     CDS             5395..6255
                     /locus_tag="ACHATZ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8403423.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            6429..7202
                     /locus_tag="ACHATZ_000874"
     CDS             6429..7202
                     /locus_tag="ACHATZ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8403424.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            7349..7714
                     /locus_tag="ACHATZ_000875"
     CDS             7349..7714
                     /locus_tag="ACHATZ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8403425.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(7862..9070)
                     /locus_tag="ACHATZ_000876"
     CDS             complement(7862..9070)
                     /locus_tag="ACHATZ_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8403426.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(9089..9367)
                     /locus_tag="ACHATZ_000877"
     CDS             complement(9089..9367)
                     /locus_tag="ACHATZ_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403427.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            9560..10216
                     /locus_tag="ACHATZ_000878"
     CDS             9560..10216
                     /locus_tag="ACHATZ_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8403428.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(10658..11455)
                     /locus_tag="ACHATZ_000879"
     CDS             complement(10658..11455)
                     /locus_tag="ACHATZ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8403429.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(11452..12636)
                     /locus_tag="ACHATZ_000880"
     CDS             complement(11452..12636)
                     /locus_tag="ACHATZ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8403430.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(12661..13908)
                     /locus_tag="ACHATZ_000881"
     CDS             complement(12661..13908)
                     /locus_tag="ACHATZ_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8403431.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            14123..15868
                     /gene="dnaX"
                     /locus_tag="ACHATZ_000882"
     CDS             14123..15868
                     /gene="dnaX"
                     /locus_tag="ACHATZ_000882"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8403432.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            15888..16202
                     /locus_tag="ACHATZ_000883"
     CDS             15888..16202
                     /locus_tag="ACHATZ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8403433.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            16227..16823
                     /gene="recR"
                     /locus_tag="ACHATZ_000884"
     CDS             16227..16823
                     /gene="recR"
                     /locus_tag="ACHATZ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8403434.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            16887..17531
                     /gene="tmk"
                     /locus_tag="ACHATZ_000885"
     CDS             16887..17531
                     /gene="tmk"
                     /locus_tag="ACHATZ_000885"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8403435.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            17546..17875
                     /locus_tag="ACHATZ_000886"
     CDS             17546..17875
                     /locus_tag="ACHATZ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8403436.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            17889..18827
                     /gene="holB"
                     /locus_tag="ACHATZ_000887"
     CDS             17889..18827
                     /gene="holB"
                     /locus_tag="ACHATZ_000887"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8403437.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            18863..19687
                     /locus_tag="ACHATZ_000888"
     CDS             18863..19687
                     /locus_tag="ACHATZ_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8403438.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            19680..20027
                     /locus_tag="ACHATZ_000889"
     CDS             19680..20027
                     /locus_tag="ACHATZ_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8403439.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            20096..20968
                     /gene="rsmI"
                     /locus_tag="ACHATZ_000890"
     CDS             20096..20968
                     /gene="rsmI"
                     /locus_tag="ACHATZ_000890"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8403440.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            21077..22198
                     /gene="dinB"
                     /locus_tag="ACHATZ_000891"
     CDS             21077..22198
                     /gene="dinB"
                     /locus_tag="ACHATZ_000891"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8403441.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(22252..22854)
                     /gene="nrdG"
                     /locus_tag="ACHATZ_000892"
     CDS             complement(22252..22854)
                     /gene="nrdG"
                     /locus_tag="ACHATZ_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8403442.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(23169..25358)
                     /gene="nrdD"
                     /locus_tag="ACHATZ_000893"
     CDS             complement(23169..25358)
                     /gene="nrdD"
                     /locus_tag="ACHATZ_000893"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8403443.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            25684..26451
                     /locus_tag="ACHATZ_000894"
     CDS             25684..26451
                     /locus_tag="ACHATZ_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403444.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            26441..28483
                     /locus_tag="ACHATZ_000895"
     CDS             26441..28483
                     /locus_tag="ACHATZ_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403445.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9145 a         5140 c         5980 g         8309 t
ORIGIN      
        1 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
       61 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
      121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
      181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
      241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
      301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
      361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
      421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
      481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
      541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
      601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
      661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
      721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
      781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
      841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
      901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
      961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     1021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     1081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     1141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     1201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     1261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     1321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     1381 ttgagaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     1441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     1501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     1561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     1621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     1681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     1741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     1801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     1861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     1921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     1981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     2041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     2101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     2161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     2221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     2281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     2341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     2401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     2461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     2521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     2581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     2641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     2701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     2761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     2821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     2881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     2941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     3001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     3061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     3121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     3181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     3241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     3301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     3361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     3421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     3481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     3541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     3601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     3661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     3721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     3781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     3841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     3901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     3961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
     4021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
     4081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
     4141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
     4201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
     4261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
     4321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
     4381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
     4441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
     4501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
     4561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
     4621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
     4681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
     4741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
     4801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
     4861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
     4921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
     4981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
     5041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
     5101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
     5161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
     5221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
     5281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
     5341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
     5401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
     5461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
     5521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
     5581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
     5641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
     5701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
     5761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
     5821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
     5881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
     5941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
     6001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
     6061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
     6121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
     6181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
     6241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
     6301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
     6361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
     6421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
     6481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
     6541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
     6601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
     6661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
     6721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
     6781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
     6841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
     6901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
     6961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
     7021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
     7081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
     7141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
     7201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
     7261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
     7321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
     7381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
     7441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
     7501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
     7561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
     7621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
     7681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
     7741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
     7801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
     7861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
     7921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
     7981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
     8041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
     8101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
     8161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
     8221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
     8281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
     8341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
     8401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
     8461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
     8521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
     8581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
     8641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
     8701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
     8761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
     8821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
     8881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
     8941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
     9001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
     9061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
     9121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
     9181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
     9241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
     9301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
     9361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
     9421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
     9481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
     9541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
     9601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
     9661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
     9721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
     9781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
     9841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
     9901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
     9961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    10021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    10081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    10141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    10201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    10261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    10321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    10381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    10441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    10501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    10561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    10621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    10681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    10741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    10801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    10861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    10921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    10981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    11041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    11101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    11161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    11221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    11281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    11341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    11401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    11461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    11521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    11581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    11641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    11701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    11761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    11821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    11881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    11941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    12001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    12061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    12121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    12181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    12241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    12301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    12361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    12421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    12481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    12541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    12601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    12661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    12721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    12781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    12841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    12901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    12961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    13021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    13081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    13141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    13201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    13261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    13321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    13381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    13441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    13501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    13561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    13621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    13681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    13741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    13801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    13861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    13921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    13981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    14041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    14101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    14161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    14221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    14281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    14341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    14401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    14461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    14521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    14581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    14641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    14701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    14761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    14821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    14881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    14941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    15001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    15061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    15121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    15181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    15241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    15301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    15361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    15421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    15481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    15541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    15601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    15661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    15721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    15781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    15841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    15901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    15961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    16021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    16081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    16141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    16201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    16261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    16321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    16381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    16441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    16501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    16561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    16621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    16681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    16741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    16801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    16861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    16921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    16981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    17041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    17101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    17161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    17221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    17281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    17341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    17401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    17461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    17521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    17581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    17641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    17701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    17761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    17821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    17881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    17941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    18001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    18061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    18121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    18181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    18241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    18301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    18361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    18421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    18481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    18541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    18601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    18661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    18721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    18781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    18841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    18901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    18961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    19021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    19081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    19141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    19201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    19261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    19321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    19381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    19441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    19501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    19561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    19621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    19681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    19741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    19801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    19861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    19921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    19981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    20041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    20101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    20161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    20221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    20281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    20341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    20401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    20461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    20521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    20581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    20641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    20701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    20761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    20821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    20881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    20941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    21001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    21061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    21121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    21181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    21241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    21301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    21361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    21421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    21481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    21541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    21601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    21661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    21721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    21781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    21841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    21901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    21961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    22021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    22081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    22141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    22201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    22261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    22321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    22381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    22441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    22501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    22561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    22621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    22681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    22741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    22801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    22861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    22921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    22981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    23041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    23101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    23161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    23221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    23281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    23341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    23401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    23461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    23521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    23581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    23641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    23701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    23761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    23821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    23881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    23941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    24001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    24061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    24121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    24181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    24241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    24301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    24361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    24421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    24481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    24541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    24601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    24661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    24721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    24781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    24841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    24901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    24961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    25021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    25081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    25141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    25201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    25261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    25321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    25381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    25441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    25501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    25561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    25621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    25681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    25741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    25801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    25861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    25921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    25981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    26041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    26101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    26161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    26221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    26281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    26341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    26401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    26461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    26521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    26581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    26641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    26701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    26761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    26821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    26881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    26941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    27001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    27061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    27121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    27181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    27241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    27301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    27361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    27421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    27481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    27541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    27601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    27661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    27721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    27781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    27841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    27901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    27961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    28021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    28081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    28141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    28201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    28261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    28321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    28381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    28441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    28501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    28561 tttttgtcac aacc
//
LOCUS       DAYYAJ010000024        27790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000024 DAYYAJ010000000
VERSION     DAYYAJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.024"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..447
                     /locus_tag="ACHATZ_000896"
     CDS             10..447
                     /locus_tag="ACHATZ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403446.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            542..1426
                     /locus_tag="ACHATZ_000897"
     CDS             542..1426
                     /locus_tag="ACHATZ_000897"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8403447.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1650..4790
                     /locus_tag="ACHATZ_000898"
     CDS             1650..4790
                     /locus_tag="ACHATZ_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8403448.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIADNYSAEIT"
     gene            4885..5382
                     /locus_tag="ACHATZ_000899"
     CDS             4885..5382
                     /locus_tag="ACHATZ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8403449.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5401..5865
                     /locus_tag="ACHATZ_000900"
                     /pseudo
     CDS             5401..5865
                     /locus_tag="ACHATZ_000900"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5862..6620
                     /locus_tag="ACHATZ_000901"
     CDS             5862..6620
                     /locus_tag="ACHATZ_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8403450.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6706..7446)
                     /locus_tag="ACHATZ_000902"
     CDS             complement(6706..7446)
                     /locus_tag="ACHATZ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8403451.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7507..8760
                     /locus_tag="ACHATZ_000903"
     CDS             7507..8760
                     /locus_tag="ACHATZ_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8403452.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9082..9309
                     /locus_tag="ACHATZ_000904"
     CDS             9082..9309
                     /locus_tag="ACHATZ_000904"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8403453.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9432..9758
                     /locus_tag="ACHATZ_000905"
     CDS             9432..9758
                     /locus_tag="ACHATZ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8403454.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9826..10539
                     /locus_tag="ACHATZ_000906"
     CDS             9826..10539
                     /locus_tag="ACHATZ_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8403455.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10849..11700
                     /gene="licT"
                     /locus_tag="ACHATZ_000907"
     CDS             10849..11700
                     /gene="licT"
                     /locus_tag="ACHATZ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8403456.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11821..13686
                     /locus_tag="ACHATZ_000908"
     CDS             11821..13686
                     /locus_tag="ACHATZ_000908"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8403457.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13700..15142
                     /locus_tag="ACHATZ_000909"
     CDS             13700..15142
                     /locus_tag="ACHATZ_000909"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8403458.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            15250..15951
                     /locus_tag="ACHATZ_000910"
     CDS             15250..15951
                     /locus_tag="ACHATZ_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8403459.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16090..16602
                     /locus_tag="ACHATZ_000911"
                     /pseudo
     CDS             16090..16602
                     /locus_tag="ACHATZ_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            16613..17119
                     /locus_tag="ACHATZ_000912"
     CDS             16613..17119
                     /locus_tag="ACHATZ_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFM8403460.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            17530..18105
                     /locus_tag="ACHATZ_000913"
     CDS             17530..18105
                     /locus_tag="ACHATZ_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8403461.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18105..18545
                     /locus_tag="ACHATZ_000914"
     CDS             18105..18545
                     /locus_tag="ACHATZ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8403462.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            18561..19262
                     /locus_tag="ACHATZ_000915"
     CDS             18561..19262
                     /locus_tag="ACHATZ_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8403463.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19760..20185)
                     /locus_tag="ACHATZ_000916"
     CDS             complement(19760..20185)
                     /locus_tag="ACHATZ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403464.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            20416..21741
                     /locus_tag="ACHATZ_000917"
     CDS             20416..21741
                     /locus_tag="ACHATZ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8403465.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            21743..24043
                     /locus_tag="ACHATZ_000918"
     CDS             21743..24043
                     /locus_tag="ACHATZ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8403466.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            24050..25021
                     /locus_tag="ACHATZ_000919"
     CDS             24050..25021
                     /locus_tag="ACHATZ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8403467.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            25043..26440
                     /locus_tag="ACHATZ_000920"
     CDS             25043..26440
                     /locus_tag="ACHATZ_000920"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8403468.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            26596..27219
                     /locus_tag="ACHATZ_000921"
     CDS             26596..27219
                     /locus_tag="ACHATZ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403469.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
BASE COUNT         9264 a         4632 c         5965 g         7929 t
ORIGIN      
        1 agtagataaa tggagtggtt ttgcaaattt cttttatttc tagaagttat tcacgtatac
       61 ggatttgttt ttctcacaaa taaaaaggca ggaagtacaa tggatcattt gtatataaaa
      121 tatattcatc aattttccgt tttggtgtta ggcacactaa ttatagcggg gattgtcgca
      181 tttattattc aaggccgtga catattaaca gtacatcaag agtttttcag ttatgcattt
      241 ggtattcctc tgatcgctat aaggtctgat aacaaaaagt ggaagaaacg ctattacatt
      301 ctcttgctat ttttgtgttt ttatctgcca cctaagattt ttactttatt aggtatacaa
      361 tgcctatcat tttcttttat ttcctatatc attgttttaa ttttcctcag tttcatagta
      421 tggatgtttt atcttgtcaa aaaataaatg aatgtttcat gaacagtact tcatcacagc
      481 ctaatttttt tctttatgct atcgttatat taaagaaatc aatcagaagg gaaagaaagt
      541 gatgaaaaaa gttttattag tggtaaaccc cagttcaggc ggtgaacaag caaaggaatt
      601 tgagcaatta gcgatagcaa aattggaatc ggtgtttgat gaagtggtcg tcttgcatac
      661 aaaaaaagca ggggatgcaa aaaactttac tcgcgaagcg gctacggaag ggtatcatag
      721 tgtgtttgtc atgggcggag atggaacagt caacgaagga atcagtggaa tcgctgaaca
      781 agaacatcgg ccaaatttcg gatttttccc attgggtacg gtgaatgact tggctcgagc
      841 attagggata ccgctagaac ctgaggaagc gatcaatcat ttttcgattg aatcagtcaa
      901 gccattggat attggaaaaa taaatgacga ctattttatg aatgtcgtcg caattggctc
      961 tatccctgaa tcaatcaatg atgtagatgc ggagaagaag acgaaatttg gaaaactagc
     1021 ttatttcatg tcaggaatca aacagttagc ttccacacaa tcctactcgt ttcacgtaga
     1081 agttgatgga aagaaggaag aaattgagag cagtaccttg ctgataggac taactaactc
     1141 agtcggcggt tttgaaacat tacttccaaa tgcaaaagtg gatgatggta agctccattt
     1201 ggtttattta aaagattctt ctttgatcga tacgcttaaa acattgccag acctattaaa
     1261 aggagtagat gaatctacga aaaatctagc ctaccttact tgtgagcaat tagttgtgtc
     1321 attagctgat gaggaagaat tgacgacgaa tgttgatgga gatgaagggg ataaattacc
     1381 tgtgacaatc aaagtattgc cctcacattt acaggtttat tgttaaaatg gaggttgtga
     1441 ccgaggagtt ggcgggagaa caccgcttgt tcgggataag ggactgtgac aagacttttg
     1501 tcacagcctt ttttttgtac aaaaaagaaa tttacaccta atagaaaaat ttttccataa
     1561 aaatacatat tttaaatatg aaagtacaat tggaaataga ttaaaacgat aaaataaaat
     1621 tgtcaaaacg tcagagagga tggtggagaa tgaatcaatg tgtaaaaaag ctgattgcgt
     1681 cgattttgtt tgtcttccct ctcttgatgg tgacatccgt ttttgccgat gtagagaaaa
     1741 caaacgaatc aacgcaatca gagaatcaaa gttttgacaa gactactttt tcagaagaat
     1801 cgattacttc tgaaactaca gaatctcaaa gtgaggcttc tactaataaa aggcaagaag
     1861 aaaataagac aagtgagacc tcagaagtag ctgaacaaac gtctgatagc agtgaagcgg
     1921 cactggcaag agctgtttca gcggatgggt acagcgaagc tgccacaaca gaagaactgg
     1981 cgcagcttct cgcagacgaa tcagtaacta agatccgact gattcagcct ttgactttag
     2041 acagggaatt agagatcaaa cgagatatcg ttattgattt tggtggattc gctcacaatt
     2101 tcggcacaca ccatatttat atcaatgaga tacctcagtc gatcgagttc cagaatttta
     2161 aaggaacagc tgcacatcca ggtggattga caccaaatat cgatggaaat gccattatca
     2221 ttgcttatat gaccgatttt tggggaagca gatatcattt tacgggagaa ataaaattta
     2281 ccgggacact tgatttgtat gatgggtcca aattaggact gatttacgct cctcgtgcaa
     2341 ctgtcacatt agatggtgtt tccggtgtgt tggatgtcca accagtgaag gaaggactga
     2401 atgccgctcc gggtaaagcc tatttttgta gaagctatca attgaatgtg atcaatggta
     2461 gtcaacttta tggaccgtat ctagggaaat tctacggttt tctagattca ggagatgaga
     2521 ctggtagtgg aaaaacagcg ccaggaatcc atatcttaag cggttcaaag gtctctcttg
     2581 attatgatcg ggcagatggc gttagcgatg aaggagaggc agtcgatacg ctacctggta
     2641 atgtcatgtt tgaagtcagt ggttcgggaa gcgaattttc ggtaaataca aagatcaata
     2701 ttaccaatga caacaatcgc ggaatcatcc aaatgagagg ttcaggatcg aaagtcactg
     2761 tatcgaatgg tgggaaaatc accatcaata cagcaactac gggcggattt cgcttgcaag
     2821 gagattctag tcaaatccat gtatcaagtg gtggacaaat ctcggtcaaa atggctggag
     2881 atgacgatca acccgggaac aatgggatgc gttttgtagg aaaaggcctt tctttgatcg
     2941 tagaaggagc cggatcagtt atcgacatcg ataagcagag tgggcgttcc agcgcagttc
     3001 gttttgagaa tggtacgcaa aagctgacag taaccaatgg aggctcattg agggtaagaa
     3061 atacaggtga tggcaaatct tacgtgaaca gactcaatca aggaaatcaa gcgatccaat
     3121 tttatgacgc ttctggtttt ctagaaagtc caggttcagc tgattttaca gtcactggag
     3181 aaaaatcaaa tatcgatata cgcgcagatt ccggagcaac tgtagataca actggagata
     3241 ttgatttaaa ttttacggca ggtgaaaaaa cttatttagt catggtcggg aaaacagggg
     3301 aagataagac cggcatcatc agtggggatg tgttgaatgt ggacttagaa tctcctacct
     3361 attttgattt ccaaaacaaa cggattggaa gcgagaccaa tggcataggc ggctgggtat
     3421 tccaaggaaa tggagattct tctttaaaga tcgcccattc agaaatcgct ttatggcgaa
     3481 aaacaggcgc aacattcgat aactttgaaa aagacccgga ttatttttct gatatgacag
     3541 acttatcgat caatgggacc gacttaggca atttcgtatc aagccctagt gcaggtttaa
     3601 caaatgaatt tgctcagaca ggtgccggaa tgaaaaatta taatcgaatc agtgccaata
     3661 atcagctccc gatgattgaa tccttaagag tgccaacgaa tgcagataaa aaaatctatg
     3721 ggcatgtcgt tgttcccgaa ggtgtcgagg caaagccaag agatgcttgg acaggcgaag
     3781 tagaagtgac tttacgtatt acttacgcag atacgacgaa atcatctgta gaaattactg
     3841 ctctcacgca aggacaaaca gatgaaaatg aaggggcagg atataaccca tggggagaag
     3901 gcgaacaagg cggcttgttc gaagttcaag tacccaatga tgagagatta aaaacggagg
     3961 atcaagtgca agttgttgct gctaaaaaag taagaggtgg ccaagaagtg acagaactgg
     4021 actctgccaa aacgacagtc gatgtgattc ctcctgaacc tgctaattta agtaccacat
     4081 tgatcaatgc agcttctcgc acgatcacag gtacaggtga agcaggagcc ttggtttcat
     4141 tgaaaaaaaa tcaagctgtt ttgtcagaaa caactattga tgatacaggg gaatttgttc
     4201 tttctatccc agaaaatcaa ctttttgcag gagatgtatt agaaattgta ttaaacgacc
     4261 aagcaggaga agctactgct aagggtgtgc agaacaagcc aagtaccaac gatgaaattg
     4321 ggaatcacaa tccaagtgat acgccagttg tttatcatga tgccccgcct tttgcacctg
     4381 ctacgaagat cgtagtagaa ggtggtctct catttcttgc cccttccaaa ctcgattttg
     4441 gtcagatcaa agcaactggt ttgacacaag aagcatttgg ccaaatggat atacaggaaa
     4501 aattatggat ttatgatcag cgagaaacga aaaatccgtg gatcttaacg ataaaattat
     4561 ctcagccttt cacaaatgat gcaggatttt ctatgcagga tgtcctatat ttcaagaaag
     4621 gagaggagta tcaattagtg aatgaagaag aactggaaat tgtcagagga caaaattcaa
     4681 cgaatgtgca agcagattat tcagaatatt taaataacgg tcgggcgttt aaacttgtct
     4741 taaccaaaga aaatcagata gctgataatt actcagcaga gataacatag acattagcag
     4801 atgcgccgac agaataaaga tcaaggagga tgattgatga atcgaatatg gaataaaagt
     4861 catcgaaaca actgctcttt acttatgatc cttctccttg gattcatcct ttggcagaca
     4921 cctgtccatg cctccgaatt caattttgcg gtagattttg aattaccggc taatcagatt
     4981 gataagaaca aaacatatat aaatgtaagg atggagcctg ggaaaacaca ggaaatcccc
     5041 tacacggtct ttaaccgaac agataaatca gtaacagttg agacattgat ccactccgct
     5101 acgacgaatt caaacggtgt gatcgaatat ggagaatcaa aaagtaaatt tgatgaaaca
     5161 ttaagttatg atatgaaaaa cttagcaaaa acagtgggag aaataactgt tcctcctcaa
     5221 agtgcggtca ctgagacact ttcactgaaa gcgccagaaa aagagctggc tggtgtggtc
     5281 gcaggaggga tcacgtttca agaaaaaaaa gagaacgttt ctaacaaaag tacagttggt
     5341 gtcgtcaatc agttttctca agtgaaagca atcttgattt gaaatagcga aaaagagtct
     5401 tccccagatt tgaaattaaa aaaagcttat gcggatcaaa gcgatggaag aaatgtgatt
     5461 aaggcgaatc ttcaaaatcc aaatgcttct tatatgttcg gcttgactac atcggttgaa
     5521 atctacaaaa aagaaacaaa agagccttac ctttcaataa aaaaagaaaa aatagatgta
     5581 gcacccaata caaattttga tttacttgtc ccattagaag gaaaaaaatt agctccggga
     5641 cattatcaag cggttgtgaa gggtgcttgg aaagaaaaac gctggaatct ccataccgat
     5701 tttgaaataa ctgatgatgc agcggaccaa ttgaatgaaa aggatattga tttaaaaaat
     5761 cagccagaaa catttgactg gcgatggctg atacttgttg gagtcattct tattggctta
     5821 tttgctggat tatttatttt aaaagaaaga aggaaaaaga aatgaaaaaa acaattgcat
     5881 taagtaccgt tctagctgga agtctattat tatgtacaca aagtgttttt gctgagaata
     5941 agagctcagc cactacagaa ggaacgattt ctgtgaccaa accagatgct gcaaatcctg
     6001 aagacgttgt tccagttcct cctgttgatc caaatgatcc tgatactcct tttacacctg
     6061 acgaaccaaa tcagggaaca tccggattac tgacgattga ccaagctcca gatcttgatt
     6121 ttggaacaat caagttaggt acagcaaaaa cagtatatgc tgcattacaa agcggaacgg
     6181 acagtactgg agcacaaaca caagtacaga atttcgtaca agtaacggat aaaagcggaa
     6241 attatgccgg ttggaaatta tcagtcaaac gaacagaatt tattggtaca acatctgata
     6301 cgaataaact aactggttca aaggtgaaat tcaaaaacgc cgtgattcga aatagcgcaa
     6361 ctaatagcag tggaatgcca tctaccgttt ctggaactgg cgctgaagga atcgaaatcc
     6421 cagcagatca ggcagtagat ctagtaactg caggagcaaa tgaagggatt ggcacatgga
     6481 tttattcatt aggagagaat gtggaacaag ggagacaatc agtggaatta tttgttcctg
     6541 tatccaatta tgctgtagac aactacgttt caacattcac atggtcgtta acagatgctc
     6601 ctacagaaga ggaagcgtaa aatcatatca acgacttcag ggagtggagc cgattcaagg
     6661 cccactccct gaaatctgct atgcttacgt ctcagctctt attcatcaac tcgaagcagt
     6721 tttttggaag tcatggaaac gttcctttgt cttttcaaag atctttcgac caacgagtaa
     6781 ggagtcaccg ttgaaaaatt ctaacacata atgagcagaa tcaattttct gcaattgcga
     6841 taagttgata cagtaagatt ttaaaagtaa aaagtaagca tacttttcta actctgtctt
     6901 caattgtttt aaagaagtat tcactaattt atcttctgta gttgttttga ccataacttg
     6961 gtttttcaaa cctttgatac tttcaatata taaaatttcc cgaggataca ggacgagact
     7021 tccacctttt tgatcaataa agattttatc gacatttacg atctgctgtt ctttccaatt
     7081 gatctgttct agcacttgac gaatttgttc agataaattc ctgtcttttc tgatatatcc
     7141 aatcggaagg attttccgat tgatgatctc tactgcaaaa ttcgtataag cggtgaaaaa
     7201 aacaatatta ctatgtgcat tttttttacg tatttgttcg gcgacatcaa tacctgtgag
     7261 tgattgcttc aagtcgatat cgataaagaa aacatcgtga ttatcaatag tgcccaactg
     7321 gatcaactga ttgatttgag agatagaagt gagctgcacg atattctcga tatgatgatt
     7381 gaccaattct ctgatgatcc attcgctgtg aaactggtta tcttctatga taaaatactt
     7441 tttcatatgc aggctcctat taaaaaaata tgtgtacatt ttgaaacaaa acaatggagg
     7501 aataagatgg tcgcaacagt gacactttat ttgaccttta ctttgatttt agatttaggt
     7561 atctatttat tcttttcgaa acaactgcta gtaaaaaaca gctttggttg tatagctttg
     7621 atggtggtga cacttttact tcctatccct aaagtgagct ttccttacct aattggctta
     7681 ttattgatgt ttctttttta ttttactacg aaaaacctgt ttatcagctg tttgatcagt
     7741 aatctttttt ttgctatcac tggactcagc ttttttctgg tttttgatct gatggattat
     7801 ttaggaaaga gacatcttgg aacagcaagt ttttttgcac tacttatcgc atttattttc
     7861 tatctattag taaaaagtat agacaaacag acgagatttc tcaacttgct ttttacttat
     7921 ccaactgaac actattttgg aggtatttgt attttattaa gctggggcgt gatttacttg
     7981 gtattgggaa gtgttcactt ccgctcatct aattatctgc ttctatctct tttgtcgata
     8041 gtcatcagtt ttttttcttt gtttttttcc attatgttga tcagtttcca agtaaaagaa
     8101 agacaacatc aggaatcgct tcaactgtat caaataaatg aagaatatta ccatcatcta
     8161 gaggaattca aacatgatta taaaagtcta ctgttttcat taaaaattac tcttcaggaa
     8221 aataaaaaag aaacgttgga atttttagaa atattagaag atcattctgc agctattttt
     8281 gaaaaaccgc aaatacaaca gctaacaaat attgtatccc cagctgtcag gggtgtcttt
     8341 ttaaaatttc tagaaaaagc agaatcggag aaaatcccga tccaagtagt tattcctgtc
     8401 gaagtgaaaa caatcccgat tgatctggtt gtttttattc gctgtttatc tatttttatt
     8461 tctaacgcat tcgatcatcg tgtagccgat cagagtccga ttacgatcaa tctgctggaa
     8521 gaaaaagagg gatcacgttt tagtatagcg aacaaggcaa atccaacgaa tttgacgcta
     8581 tcggagatgg tcaaaagagg aaaaacctct aaaaaaaatg gcgggttagg tctatacagt
     8641 gcgaaaaaaa tgttggatgt ttacgaaaat gcagaactaa aaatcgaatt ttcaaaacag
     8701 aatcactatt tttttgtaga aatgtatctg gcatctagaa agagcaaatc agcctactga
     8761 cttactgtca aaggataagt ttgaggaaag tctacacgga gatagagaca acaacacagc
     8821 atattcaaaa gaaacagaag cgtgatgacc gttgtcaccc ttctgtttct tttgctatgt
     8881 agttaaacaa agtagttgac acttccgcta cttagtatta gtacgaaagt atacttcatg
     8941 atactaagta gataagaagt gaaaagcgga acccacgatc gtcaaaatgc taactgcttt
     9001 gcttacacca gataaaggga agagcaatat cgcttatttt aggcgtggag aagcaagcaa
     9061 atccctacga tcacaaaaaa aatgctgacc cagcagttaa gagagttaga acaagatgaa
     9121 agtatccatc gaaaagccta taaccaagtc ccgcctaaag tagagtactc gctcaccgaa
     9181 gaaggaaaat cattaagaga gattctagta gcgttgtctg tttgggaaga agaacgtatc
     9241 gaaaaacaaa aagcctcaag ggaagaagac acgctactgc atacacatgg tggctaccta
     9301 aattattgat tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag
     9361 catcatctat tttttatgta aaaaaatggt atgattaatc aagtaagaag tgattggaag
     9421 aaggcaagca aatgaatagt atggaaaaag tcaaccagtt tagagatgaa cgtaattggc
     9481 gacaatttca taacgaaaaa gatttggcta tctctatttc tttggaagca tctgaattgt
     9541 tagaactttt tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca
     9601 aagaagaact ggcagatgta ttgatctatt catacatgat ggcagacaac cttcacctgg
     9661 atctagatga gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa
     9721 aaagcgcagg tgtacgaaaa aaatacacag agttgtagaa atgtgtcatt cggctgttgg
     9781 aaagagcaaa agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga
     9841 aaagcaaaaa gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct
     9901 ttgattttca atgaacagat caaggattat tttgtcagag agacaggaga taaatatgct
     9961 atagcgaatg taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac
    10021 tttgatgcgg tagaaccaat gacgacagaa ggggtcatga ggtcgcaaat gagaggaaca
    10081 gatctaccag tgattgctag tatcgcagtc ccttcagttt caatcaattt accggttttc
    10141 aaaggcttgg ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa
    10201 atgggaaaag gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgctgttt
    10261 acaccgctag aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg
    10321 tatacataca aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat
    10381 gaagtcgagg gaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg
    10441 cgactagttg tacaaggaga actggaaagt gttacatcga taaaagatgc gacagatgat
    10501 atgagatctg ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt
    10561 acagattaaa aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa
    10621 agagaaacgc aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca
    10681 gtttttttat tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt
    10741 ttgaataaga aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca
    10801 gtaggcgaat tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat
    10861 aaaattttga ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt
    10921 atgggaaaag gactagcttt tcaaaaaaag attggggacg agatttcgcc agaagcggtc
    10981 caaaaagaat ttgttttaag agattctttt gctaaaaacc aattccagca gctgatggct
    11041 gatattccgg cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa
    11101 ctaaaagttg aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg
    11161 atcacacgag caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa
    11221 ttttatccga aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg
    11281 ggtgtggaac tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa
    11341 caaggaaacg aagacatgca agtgacgatg accgctacca caatggtaag agatatactg
    11401 agtatcatca gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt
    11461 atcattactc atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag
    11521 caagatgaat ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc
    11581 gaacggatca atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag
    11641 atttatttaa cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa
    11701 ggattgttac tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt
    11761 gactgttttt ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca
    11821 atggataatc aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc
    11881 aaaagtttag tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca
    11941 gatacgcaaa agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag
    12001 tatcaagtag tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa
    12061 ggtcttgcag tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc
    12121 gatattatct ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg
    12181 aaaggattct tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat
    12241 caaatattgt ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt
    12301 acagcagcta aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt
    12361 gtgtatccag ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc
    12421 ccagtgattc tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta
    12481 tgggttcaaa gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg
    12541 attttagttc cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga
    12601 ccaatcggga cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt
    12661 agtccaatcg tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc
    12721 atgcattggg gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta
    12781 ttgatgccaa tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca
    12841 ttgcgaacaa aagatacgaa acttcgagca ttagggattt cttctacagt gacttcgtta
    12901 tttggaatca ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt
    12961 gccgcttgta tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct
    13021 ttctccagca gtttggtcag cttattgacg attccgactt tcattaatac ggtggatggt
    13081 gtagagtcaa atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc
    13141 gtaggtacat tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa
    13201 catgcgaatg caggagaacc gattacttca gcacggcata ccttaaaaag tcctttaacg
    13261 ggtaaagtac tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga
    13321 aaaggtatcg ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc
    13381 acgactattt ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggagatt
    13441 ttgattcata tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc
    13501 aaacaagggg atctcgtcaa agcaggagat ttattgattc gttttgacat cgaagcaatt
    13561 agagcagctg gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc
    13621 gatatacttg aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta
    13681 aaataaggag gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc
    13741 aactgctgcc aatcaattag aaggggctta tgatcgagac ggaaaagggc tatccgttgc
    13801 agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag aatttgactg
    13861 gacgattgat caagaaaaat atatttaccc gaatcatcga ggaattgatc attacgatcg
    13921 tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc gtttttcgat
    13981 tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag cgggattaga
    14041 attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg tgatcacgat
    14101 ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga aaaatcgaaa
    14161 actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca gcagtaaagt
    14221 caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag ctttaagtca
    14281 aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag catggcataa
    14341 tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt cggatatcca
    14401 aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc cagtcaatca
    14461 ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg tccaagtacg
    14521 tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg atccggaaaa
    14581 attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg actacatcgg
    14641 atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag caacttcaga
    14701 agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat ggggctggca
    14761 gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt accagaaacc
    14821 attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg atggcacgat
    14881 tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg ctaatgcagt
    14941 agcagacggt gtagatttga tgggctatac gccttgggga tgtatggatc ttgtcagtgc
    15001 atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag acgatgaagg
    15061 aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag aagtaattgc
    15121 ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca cttctcacat
    15181 gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag taaagcaaag
    15241 ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa aattaaagaa
    15301 gatattttct caggaaaata tccagtcggt tcgatgttac cgacagaaag tgaattagaa
    15361 gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct ggcatcagat
    15421 gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg tccttacaat
    15481 cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata tgaagtccgg
    15541 aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt gttttccttt
    15601 ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca gccttatatt
    15661 tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga atttggcaaa
    15721 gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt ttctgatgat
    15781 ttctcggcag tatttttaac agaagaagaa caagaaattc tgaaaacaga cgcacagatc
    15841 ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga atattcggag
    15901 gcggtctacc atacggagct gcatccttac catattgatt atgaaacata aataaaaaaa
    15961 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc
    16021 tcatattagc ttctcatatt tttcgtttgg ttaggcttat taccgcagat acccggctgt
    16081 ttctggatta ccatttattc tggataaggg ggtgtggcac gattttcaga aggaatctat
    16141 gaaccaatca ctgtattgat tttagcttcg atggtaaccc cattcggctt gacgatcgct
    16201 tatttcttag ggaaaatcat tcgtaaaaat atattcaatc gtcaagaaat cgatacattg
    16261 aagaccgctt ttcctatggg gatctgtcag atcacagagg gatgtatccc tatcgtattg
    16321 aacgacttgg cacgcaatgt catagcaacg ggagtcggtg gggcagtcgg cggtggattg
    16381 agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc agtcgctacg
    16441 atgaccagac catgggcatt cattggtgca ttgttagctg gttcttttgc tacgggaata
    16501 actattttat tattgaaaaa accagttgac ccaaatgcag aagtcatcca agaagaaaaa
    16561 gaggaagaag agatatcttg ggatgacttg acaatcagtt aggaggaaca gaatggatta
    16621 caagaaaaga gtacaacaaa tgttcctaga ttcaggagta gtattgaaag aaacagaatt
    16681 agaaagaatc gaatatacag attttggatt aggagcaatc gagactgaag gattgaattt
    16741 atttgtttat gaaaataatg agcgatactg tgggaaagaa ctggtattat tgccttatca
    16801 aacttgtcca gagcatcggc acccacctcg agatgagaat gaaggaaaaa aggaaacctt
    16861 tcgttgtcga aaaggcttag tatatcttta tgtcgaagga aaagaaacaa aaaaactttc
    16921 ctgtattatt ccaaaggggc aagaacgtta ctatacggca aaaaaagaaa tcatacttca
    16981 cgctggagat cagtacacga tcgaacaaaa tattaaacac tggttccagg caggtgcaac
    17041 aggcgcggtt atcaccgaat tttcttccag cagtgatgat ccaagtgata gttttacgaa
    17101 tccagctatt tatcgttaat acaaaggttt gggaacaagc tgatgaacca aaatgatcaa
    17161 atttcgatta tttggttcgt cggctttttt atttagagaa acatcaggcg ttggtgaaac
    17221 ataacacgag aataaaatgg atttttcatt aataggagaa acaagtgtgt gactatttaa
    17281 ataatgcgtg ttattgacat gcaggacgaa atcccaattt cttctataag taagtcgttt
    17341 caattttata tttactagtt atttattttt ttggttactg taaaatagct gaaaaattat
    17401 ttttttgcaa atactatata tagtggtgtt tgatctatat accactaaaa aactacaaaa
    17461 tctagtcttt tattttaaaa aaacacgtgt agaatgaaga tagacttttg agttagggag
    17521 tgttttttta tgaaacttac tgttttttgc ggttcccgtt ttggcaataa agaaagttac
    17581 aaatttattg cgcaaacgtt aggaaaatat atggcacaag aagaaatcga actggtttac
    17641 ggtggttcat actccgggat tatgggagtg attagtaaaa cagtactgga aaataacgga
    17701 aaagtcacag gtatctatcc aaatggcctt tttgaaactg agctgcctgg gaaagatgtt
    17761 actacattta ttcctacaga gacaattgat gaaagaaagg tattattatt tgaaaaagga
    17821 gatgctgtcc tcgttttccc tggaggtcta ggtacattgg aggaattttc gcaacttctt
    17881 tcttggatag cgatcggcct gacacctgat aagccaatcg gaatattgaa tattggcggt
    17941 tattacagcg gcttgcaaca attattagaa acttttgcaa aagaggattt tatggataaa
    18001 aaatggttgg atcaagtgat cttttccaac aatccattgg aactggtcaa tgaattacga
    18061 gcagtatcta ttaaaaatca gttattattg aaggaggcaa aataatgagt caaatatatt
    18121 tagcaggacc atttttttca gaggagcaaa tcgatcgagt aagtcgtatc gaaaaagcgt
    18181 tggaggaaaa caaaacaatc acaagtttct actctccacg tcatcaccaa gaaagcaatt
    18241 atgaactatt cagcgctgga tgggcgcagg aagtatacga aaaagatatg gaagagttga
    18301 caaatgcaga atttgttttg gctatccttg actttgagca tcaaacaatc gatccaggaa
    18361 cggcttacga attaggtgtg gcaactatgt tgaaaaaacc gatgatcatc gtacaagaag
    18421 agacggtacc tacgaacttg atgattacac aaagtcttca cacgtattta aaatcagacc
    18481 aagctgtacg tgaatatgat tttgaaaaat tgcctgttga aacttacgta ggcgagtact
    18541 tatagaagag gaagggttgt atgtcagtga ttttgatttc aggaacgatt ggtgcaggta
    18601 agagcagttt gacggatatg cttgcaaaag agattgattc taaaccattt tatgaaaatg
    18661 tcgaagataa cgaagtattg ccgctttttt attcaaatcc tgagcagtac gcatttttat
    18721 tgcaaatctt ctttttgaat aagcgatttt tagcaatgaa aaatgcttta gtgaacgatg
    18781 acaatgtgct agaccgctcc atctacgaag atagtctgtt gttccatcta aatgctgatt
    18841 taggtcgggt aacggatatc gaagtacagc agtacgatag cttattagat acgatgctaa
    18901 acgagttaga tgatgttgct ccaaaaaaac gtcctgattt gatggtccat atcaaagttt
    18961 ctttggacac gatgctagaa cgtatcaaaa aacgcgggcg tgactatgaa cagctagaaa
    19021 gcgatgaaac gttatacacc tattacgaaa cactgaacac ccgctataat caatggtatg
    19081 aggattttga tgtatgtcct aaaatacaag tggatgggga tacattcgat tttgtagaaa
    19141 atccagaaga tgcaaaaaag gtgattcgtc agatccaaga aaaattagct gaattggaag
    19201 gaaagcctgc tggaaaatat tttacagata atcagcatgc gaaaggtcaa gaattaggct
    19261 aaaagtaaaa aaccaaacga cgggaaagaa aaattactac gtgatgtaga ataagtcttt
    19321 ctatcgtttg gttattttat attcaaagat gtgaaataaa taaaacgtta ctctttgatt
    19381 tgtgaactga aatgatccac tgtttgtttt actgattcca gtttttgaag cttgatcaaa
    19441 ggaagaagct gagtttgaag ttcataaaaa tcagccatag tgagttttgc ttgattttgc
    19501 ttatcttctt ttgcttttta cataattttt gacaggataa gattcaaacg atttaaaaga
    19561 atctttgact agtttgtggc aattgtcgca tatactcaaa cttccttgat cattaaattc
    19621 tgtatggata tttttgttgt tattacatgt acaataatca ggactatcga tcatccattt
    19681 cgtctccttt acagtcatct tgcttcttta ctatagcaga aaagaagggg agaaacaaaa
    19741 gaagatccaa cagatggcac tatagactgt agatgttgcc atccttatcc ttcaaacggt
    19801 aattaccgat tttggtaaat gtgactgttg cttctttttt attttctttt cctgaaataa
    19861 tatattcgtc atctttggta gtaatcttga tatctgtcag cactgttttt tcttcagatg
    19921 attcagaaga atcagctcct tgtgtctgat acgtgcgtgt ccattccatt tttttatctg
    19981 taaatgtcaa ctgtcccgcg gataaatcca tatctttacg tacttttagc gtatatacac
    20041 tgttggtgat atctgctttt tccgctttta agacagaaga atcggttgtt tcgtccgagg
    20101 attggtcaga gccgccaaac aaatcacagc cagtcaatgt caaaagtccc gcagacaaaa
    20161 taaatagtac gattaatttc ttcaaaatac ttcccccatt tgataggata aagaaattat
    20221 accattggaa agtaaaactt caaacggatg gaagagaaaa aattcacaaa ataccattaa
    20281 atagaataat ttttcaaaag tcataaaaaa tccaccacct ttagaaagcg ttttaaaaaa
    20341 atgtgttgca atctttaaaa aaagtgctat attaaaaatg aaccggttca attttagaat
    20401 ctaggagatg aaataatgaa agtcgtagac agactatctg aaaaaatgct accaatcgta
    20461 tctaagatga gtagccagcg acacttgctg gccattcgag acagttttat cgttactatg
    20521 ccgttagtaa tggctgcttc tgtcatggtt ttgctgaatg ctctgatttt ttcaaatgca
    20581 acagtccaaa gaatacttga tctaagtcca ttggctgaac ttgcttcaat cgtgaataat
    20641 ggtacgatga gtattttagc tattatcgtg tgttacaata tcggctggaa tttggcaacc
    20701 cactatatcc aaacaggcgt gattgatgat ccatcattta gtcctgttca tgcaggaatt
    20761 ctaagtacag ccgtaatgtt tattttgatg tctttatcaa gtacagtcac tttgatggac
    20821 ggaaatacag cagaagctac aggggttttc ttacaaagtc tgacttcttc gtctggctta
    20881 gcaacggcga tgattgctgc ccttgtcagt acagaattat ttatcaaatt agcgaaaatg
    20941 aagagtttta aaatcaggat gccagaagga gttccaccgg cagtagctgc gtcatttaat
    21001 tctttagtac cagaaatagt tgtcatttta tttttttcag ttgtcgtttt tactttaaac
    21061 catacgactg gtttgaatgt tccgcaatta attgaattag cgattcagac accattgaaa
    21121 gcttttgttt tatccgtccc aggcatctta tttttacagt ttttctctga tttcttatgg
    21181 gtgtttggta tgcatggttc gagtattttg gctcctatac ggcaagcacc tttgttacag
    21241 tctattcagg aaaacatgga tgctttttca gtaggaaaag aaattcctaa tattattacg
    21301 gagcccttta ccaatgcatt tggattgatc ggaggtggcg ggtgtatttt accgttagtt
    21361 attgctatct tgtgggcttc taaacgtcaa gaacagcgat caatcgctaa atttggtctg
    21421 actacttgtt tatttaatat tacggaacca attatgtttg gattaccagt agtcatgaat
    21481 ccaatatata tgattccatg cgctattata ccatctatta acttggttat tgcctacgca
    21541 gccacaagtc ttggaatcat cagtaaaaca gttgctgctg caccttggat cactccaccg
    21601 gttatacaat cttttattgc tacaggaggc gatatacgag cagcagtctt aacagtaata
    21661 cttatcattc tagatgtatt tttattcctg ccatttgtat tagccgctaa taaggcaaaa
    21721 ttggcagaag gaggatatta aggtgtacga ttacccgtat caaaacccaa agttaaccat
    21781 ccgccaaaga gtagaagatt tactttcgag gatgaatatt gctgaaaaag taggacaggt
    21841 aaaccagcat ttgtatggtt gggaatgtta tgaaaaaaac ggagacaaga tcgaattaac
    21901 agaaaagtta aaagaacatg ttaaatgggg aggcggtctg ggtgccttat acggtctgtt
    21961 tcgctctgat ccgtggtcta aaacagatta taagaatggg attcctgcaa aagaaagctg
    22021 gaaggtggct aacctagtcc aagaatatgt actacagcat tcgcgatgga aaattccagt
    22081 tttgcttgta gaagaatgtc cacatggtca tcaaggcttg gatgggattt cttatccaac
    22141 gaatatcggc cgtggaaata ctttcaatgt agcattatta gaacaatcga gtcgattgat
    22201 ggcaaaagaa ctggctgcta aaggtgttca tttagcgctg gtgtcgactt tggatctggc
    22261 aaaagatcct cgttggggaa ggacagaaga gtgctttggg gaagatccga ttctctcagc
    22321 tcgtttttct gaagcggtcg ttcgagggtt ccaaggagag attatttcag aaaaagtttc
    22381 ttttcttgat cagacagtag aggaaatcaa taagaagaag gatcagatcg gtgttgtgtt
    22441 aaagcactgt atcgcacaag gagaagcttt aggcggtcat aattcaggca cagtaacgat
    22501 tggacgcaga gagttctccg atatttacgg accattattg aagagtacaa gaaatgcagt
    22561 aggtgtcatg gctgcttata atgacatcga cgggatacct tgtcatatca atcgagcgct
    22621 ttttgagcaa acattaagaa aagagatcgg ttatcaagga atcgttatgg cagacggtgt
    22681 ggctttggat cgtttatccg atgtttttac agataaaaag actgctgcag catatgcatt
    22741 agcggcagga atcgatctca gtttgtggga tgagacctat accaagattg cagaagccat
    22801 tgataatcaa gtcgtagatg aaaagctact agatcaagca gttcgccgag ttttatctgt
    22861 caaattttta ttaggtttat ttgaacagcc atttgcaaac gatccaaagg agtattggga
    22921 tcatctcatg gaggaatcag agcacttaaa tttagagaca gcaaaagaaa gtatcacttt
    22981 actaaaaaat gacggtatat tgccgttgga tcaacaagta aaaaacattg ctgttatagg
    23041 tccaaatgct cacgatgttt accacttgtt gggagactac tctgcaccac aaacagaaga
    23101 ccgtttgaaa aaaaagatag tacaagagat aaaaacagag tttccccaat caaaggtttc
    23161 ttatgcacaa ggatgtgaag taagaaatca ggaggatcag gaagaaaaat tattagaagc
    23221 ggtgaccctt gctcaaaact cagatgtgat cattgcagtt ctaggtggat caagtgcgcg
    23281 gaactttgat atggaatttc ttcgtaacgg ggcagtttca tcgaaaggga tcaacatgga
    23341 ttctggagaa aatgtagatg ttgcttctct ttctttaggt ggataccaag aacagctgat
    23401 tgaacaattg tatcaactag gcaagccaat catcacgata cttgtgcaag gaagacccta
    23461 cgatttgatg aaaatcgagt catttacgaa tgccattgca acagcatggt ttcctggaca
    23521 ggagggtgga aaagccattg cgcagatgat ttctggaaga aataacccaa gcggacgatt
    23581 gagtttgtct tatcctagaa atagtcggca actacctgtt tattactatc agcgtgctgc
    23641 ttctaaacaa gagaattatt atgatttaag cggaagtcca ttttatcctt ttggacatgg
    23701 tctaagctat acaacatttg aatacagtca gatggaagtc aaaacggata ttgacaaagt
    23761 aataatatca attaaagtta aaaatacagg aagtgtttca ggtaaaacaa gtactcttgt
    23821 ctatgtccga ttaattgacg gcgttgtcat ccaaagaacg aaattattga aagcttttcg
    23881 taaagatgaa ttgcggtctc aagaggaaag actgcttgtt tttgaactca cttctgaaga
    23941 ctttagttat atggatattg atgacaagcg gcattatgca aagaaagcgg tcatcatggt
    24001 cgaacaacaa gaaaaagaaa tcaaatgtaa ctggcaggag tgaaaatgta tggcatcgat
    24061 ccgtgacgtg gctaagatgg ctggtgtttc agtgggaaca gtttcccgct atttaaacgg
    24121 tcagcagctg aaggaaaaga atatggaaaa aatatcagaa gcgatttcag cgttggacta
    24181 taaagaaaac atcattgcta aaggattgaa gaataatcga agtttctcta ttggactgtt
    24241 gataaacggg atatctaacc gatttggttc ggaggttgtt tcagggatcg aaagagtagc
    24301 tgaagaaaat gggtatagct tattattaag cgggtttgcc gatcagccag aaaaaatcga
    24361 acaaaaaatc gaatacctga tgaagcatgc gatcgatgga ttgatcgtct tcttatcaga
    24421 agaagagtgg tctggatttg aaatgctgac aaaaatgagt atcccagttg tcgcattgaa
    24481 ttgtccgggt gggccagcag gtattgatac aatagtagtg aatgatcgag aaagtgtagc
    24541 aaaagtgatt gctcatcatg gcggacaagg acataaaaca ataggattta ttgctgccgc
    24601 tcaaacagat tacgtagcaa gagaacgttt agctggagcc aaagaagcgg tacaaaagga
    24661 tccgtctatc catttagaga tctttacagg agactattct agaaaaagcg gctattacgg
    24721 ggcaaaagca ttattagaaa aaggggcaac tgctattttt gtcagcaatt acaacatgtc
    24781 gattggtgcc atcgaatgtt tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt
    24841 cagtcactat gattatttag ataaaaacga tctgagtatc cttccaaaaa tcacgatcac
    24901 gccaccaaca gataaaatcg gggaacgagc agcagaacag ctgttgaaac gaatcaaaaa
    24961 agaagaggaa gaaaatgaaa aagtgattgt gatggaaaat atcatacatg gaatcaacta
    25021 aaaaagaaaa agaggtgctt tgatgaaaag atatcaattt ccaaaaaatt tttggtgggg
    25081 ctctgcagca agtggtccac aaacagaagg aagaatttca ggcgatggaa aaggagagaa
    25141 tatttttgat tattggtaca aaaacgaacc gcaaaagttc tttgatcaag taggacctga
    25201 aaaaacctcg caagtttata cgaagtatca agaggatgta cagttgatga aacagactgg
    25261 gcataattca tttcgaacct ctatccaatg gagccgtttg atacctgatg aatctggaac
    25321 aatcaacgaa gaagccgttc gtttctatca tcactatttt gatgagttga ttgaaaacgg
    25381 tatcgaaccc tttgttaatc tgtatcattt cgatatgccg atgtatctgc aagaaaaagg
    25441 cggatggttg aaccgagaaa cggttgaagc ctatgaaaaa tacgcaaaaa gttgttttca
    25501 attgtttggc tcgaaagtaa aaaaatggtt tacccataat gaaccaatcg ttccagtcga
    25561 ggcaggctat ttgtatcgat ggcattatcc ggaagaatca gatatgaaaa aggcaatcca
    25621 agttggttat catgaagcgt tggcttcagc attagccatc aaagcttacc atgaaatgga
    25681 tcaaagcggt gtgatcggaa tcatcttaaa tttgacaccg agttatccaa gagacgaatc
    25741 aaatcccgaa gatgttaaag cagcacgaat agcagatgcg ttcttcaatc gttcattcct
    25801 tgatccagct gtaaaaggcg agtttcccag tgaattgatt gagagaataa aagaactcga
    25861 tctaatgccg gagattcatc cagatgattt gacgatcatt aaagaaaaca cagtagatct
    25921 tttgggaatt aattattacc aaccacgccg aataaaagca aaagaaacac caatcgatac
    25981 taaaaacggt cctatgcctg aggattactt cgacaattat gaaatgcctg gtcgtaaaat
    26041 gaatccttat cgcggatggg agatctatga aaaaggaatc tatgatattt tgatcaatgt
    26101 acgagatcat tatgggaata tcgactgttt tatttctgaa aacggcatgg gtgtagaaaa
    26161 tgaagcacga tttatcaagg aagatggtat gatccacgat gattatcgga tcgaatttgt
    26221 tcaatctcat ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt gtttaggtta
    26281 tcatatgtgg acatgcatgg ataactggtc gtggaccaat gcctataaga atcgttacgg
    26341 ttttatttca gtagatttag cacaagatgg aaaaagaacg atcaagaaat cgggtcattg
    26401 gttcaaagat gtatcagaca gaaatggatt tgaagcttaa agaatagcca agagagaatg
    26461 tatgaaaatt cccgttctta ccgcttcagg aaaaagaagt tcaattattt acgatctttt
    26521 tcaacttttt tgtctttcta tttcattttt tgtatataat agaatagtca aaattaaata
    26581 ggagtggagg aaggcatgag gaaaaaaaga ccgtttcttc tcttttggtt attcggtttt
    26641 ttcttactgc ttattttatc cgcagcattt tcaggaaaaa tgacagcaga tcttgctcag
    26701 aaagaaacag aaaaaatcgt agcaagcaaa gaatcagtag ataatttcaa aaaagaattc
    26761 tcaacttatt ggtgggagga atataaatta actgccaatt acgaaaaagg tgaattggac
    26821 atctatttgc caaaaggaaa agaagaaaat caatttatca atatcgatga cgtacttagt
    26881 gcgatttctc tcagacaata tcaattcaaa gacaaacatt tggtcgtttt gcttttgact
    26941 catgatggcg acaaggtagc acgtttgaaa aatggaaaag tggttacagc ttattctgaa
    27001 aaagtggaca aaaaagtggt ggccgatcga ctagcggaat ggaaaaagac caacgaacaa
    27061 gtcaagaaaa caactgcttc ttctcaaaaa aacgagtcga cagaactaag cccttcagat
    27121 tctggcagtg gacaaacgat aacgggaaat gaagaaaatc aagcctcaat cattacgaat
    27181 gagtcatcag aaggacaata tcaaacctat ggacaatgaa aaacagaagt tgtgacagaa
    27241 gtggtcagct tcaagaaata agaagaaatt tacgaaaagt gcttctcaag tttttgtaaa
    27301 atttcggctt atttccgaag aagctacttc tggaacaccg tgaatcagag gttgtgaaat
    27361 cagcggtttg acctgagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg
    27421 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttagtggag aggttgtgaa
    27481 aaagctgtcc tgcatcaaga aatagggagc tgcgacaaga attttgtcac agcctctttc
    27541 ctacgattag tcaagtaaaa ggtgcaagaa aaatagctcg taaacgaaag cgacaaaatc
    27601 aaggcacact aaataaagcc ccgttcgtaa aaaattttcg atctgggcaa agaagtggta
    27661 tactgtaagt ggtttagtta atggtccact gagaccgcgt acgcatagtc gtactctgta
    27721 tgtgccctaa ccatagcgtg catacatttc agaagcttgt tcatacaagc aactgtggca
    27781 accttatctt
//
LOCUS       DAYYAJ010000025        26962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000025 DAYYAJ010000000
VERSION     DAYYAJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.025"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1131
                     /locus_tag="ACHATZ_000922"
     CDS             127..1131
                     /locus_tag="ACHATZ_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403470.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1132..2076
                     /locus_tag="ACHATZ_000923"
     CDS             1132..2076
                     /locus_tag="ACHATZ_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403471.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2076..3038
                     /gene="opp4B"
                     /locus_tag="ACHATZ_000924"
     CDS             2076..3038
                     /gene="opp4B"
                     /locus_tag="ACHATZ_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8403472.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3065..3979
                     /locus_tag="ACHATZ_000925"
     CDS             3065..3979
                     /locus_tag="ACHATZ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403473.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4005..5786
                     /locus_tag="ACHATZ_000926"
     CDS             4005..5786
                     /locus_tag="ACHATZ_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8403474.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6134..6820
                     /gene="rnc"
                     /locus_tag="ACHATZ_000927"
     CDS             6134..6820
                     /gene="rnc"
                     /locus_tag="ACHATZ_000927"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8403475.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6840..10421
                     /gene="smc"
                     /locus_tag="ACHATZ_000928"
     CDS             6840..10421
                     /gene="smc"
                     /locus_tag="ACHATZ_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8403476.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10430..11239
                     /locus_tag="ACHATZ_000929"
     CDS             10430..11239
                     /locus_tag="ACHATZ_000929"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8403477.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11250..12248
                     /gene="ftsY"
                     /locus_tag="ACHATZ_000930"
     CDS             11250..12248
                     /gene="ftsY"
                     /locus_tag="ACHATZ_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8403478.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12435..14702
                     /gene="gshAB"
                     /locus_tag="ACHATZ_000931"
     CDS             12435..14702
                     /gene="gshAB"
                     /locus_tag="ACHATZ_000931"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8403479.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14791..15243)
                     /locus_tag="ACHATZ_000932"
     CDS             complement(14791..15243)
                     /locus_tag="ACHATZ_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8403480.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15393..16343
                     /locus_tag="ACHATZ_000933"
     CDS             15393..16343
                     /locus_tag="ACHATZ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8403481.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16336..17295
                     /locus_tag="ACHATZ_000934"
     CDS             16336..17295
                     /locus_tag="ACHATZ_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8403482.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17292..18047
                     /locus_tag="ACHATZ_000935"
     CDS             17292..18047
                     /locus_tag="ACHATZ_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403483.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18070..19023
                     /locus_tag="ACHATZ_000936"
     CDS             18070..19023
                     /locus_tag="ACHATZ_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8403484.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19667..19963)
                     /locus_tag="ACHATZ_000937"
     CDS             complement(19667..19963)
                     /locus_tag="ACHATZ_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8403485.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20318..20674
                     /locus_tag="ACHATZ_000938"
     CDS             20318..20674
                     /locus_tag="ACHATZ_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8403486.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20684..21583
                     /locus_tag="ACHATZ_000939"
     CDS             20684..21583
                     /locus_tag="ACHATZ_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8403487.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22103..22831
                     /locus_tag="ACHATZ_000940"
     CDS             22103..22831
                     /locus_tag="ACHATZ_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8403488.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22976..25003
                     /locus_tag="ACHATZ_000941"
     CDS             22976..25003
                     /locus_tag="ACHATZ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8403489.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25111..26208
                     /locus_tag="ACHATZ_000942"
     CDS             25111..26208
                     /locus_tag="ACHATZ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8403490.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26515..>26962
                     /locus_tag="ACHATZ_000943"
     CDS             26515..>26962
                     /locus_tag="ACHATZ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403491.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         9044 a         4666 c         5688 g         7564 t
ORIGIN      
        1 tatataatta ttaataattc attacacaaa gataagaata acctttgtta taaaacgctt
       61 tattctgtgt aatgtccttt tttattaaga aaaacttcca tttaggaagc agagaggggt
      121 attcgattgt ccgataacca attattagaa gttcagaacc tacatacagg attccgactc
      181 aaagatgact attacgatgc agtagatgac gtttcattta ctttagacaa aaacgagatt
      241 ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct tggctactac gattatggga
      301 ttacttgatc ctaataacac aaaaattaca ggtgagattt tatataacga tctgaattta
      361 atcgacctga acgaaacgct ctataataaa atccgcggaa atgatatcgg aatgattttc
      421 caagatccgt tgtctgcatt aaatcctctc atgagaatcg aagaccagat caaagaagga
      481 ctttcctatc acacaaagat gaatgcagaa cagcgtcaag ctcgtgcact agaattgcta
      541 gaacaagtag ggattcctaa tcctgcgaga gttggacgcc aatatcccca tgaactatca
      601 ggcggtatga gacaacgtgt catcatcgca atcgcaatcg cctgcaaacc tccaatcgtc
      661 attgcagatg aaccgacgac agccctagat gtaaccatcc aagcacagat tcttgattta
      721 ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct tgatcactca tgacttagga
      781 gtcgtagcag aaatggctga tcgagtggca gtgatgtatg gcggacagtt cgttgaagta
      841 gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt atacacgctc tttactgaac
      901 tctataccac aagaaggaaa tcatgatgca gaactccatg tgatcgaagg tgtcgttcct
      961 tctctgaaaa acatgcctcg taaaggttgt cggtttgcac cacgtattcc atggatacca
     1021 gcaagcgatc atgaagaaga gcctatcctt catgaagtcg caccgaacca cttggtacgt
     1081 tgctcctgct ataagcattt ccactttaga gacgaaaaag gagaggtgta gatgacagaa
     1141 ttaatcacaa tcaaagacct gaaagttcat taccctattc gcagtggatt cttcaatcga
     1201 gtaacggatc atgtatatgc tgtagatggt gtggatttta ttatcgaaaa aggaaagaca
     1261 tatggtttag tgggagagtc tggctctgga aaatctacta cgggaaaagc cgttgtggga
     1321 ttagaaaagg taacagccgg acaaatcatg tatgaaggaa aagatgtaac gaagcgaagc
     1381 aaccgcaaaa aaatgggtta taacaaggat gtgcagatga tcttccaaga ttcaatgtcc
     1441 agtttgaatc ctaaaaagcg agtgttggat atcattgctg aacccatccg aaattttgaa
     1501 cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac tgctagatat cgttgggatg
     1561 ccggaagatg cgctatataa atatcctcat gaattttcag gcggacaacg tcaacgtcta
     1621 ggagtagccc gtgctgttgc taccaatcca aaactgatcg tagccgatga acctgtatct
     1681 gctctggatc tatctgttca agcgcaagta ttgaatttca tgaaacgaat ccagcaagaa
     1741 tacggcctca gctatttatt catttcacat gatttaggtg tagtaaaaca catgtgcgac
     1801 aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg gtacaagaga agacatttat
     1861 aatgacccgc gacatatcta tacgaaacgt ctgctttcag ctattccgca aatcgatgta
     1921 gaaaataggg aaagccacaa agaaaatcga cgaagagtag aacaagagta tatccaaaac
     1981 caaaaagaat actatgatgc gactggtcga gtctacgatt tgcgtacgat cacaactaca
     2041 cacaaagtgg cgttgaaaga tggaggtgca agctaatgtg gaaaacgatt ctgcgacgtg
     2101 ttttattgat gatcccgcaa gttattattt taagtgttct gatcttctta ttagcccaag
     2161 cgatgccggg ggatccattt accggtttga tcaatccaaa tcaggatccg gcagtgatcg
     2221 aacagatgcg acaagcagct ggcttgaatg acccatggta tgtccagtac ttccgttgga
     2281 tcggaaatgc tttgcaaggc gattttggtc aaagtttctt atacaaacgc tctgttgctg
     2341 acttgatcgg cgaacggatc atcaacactt tatatctatc gatcttgaca gttgtcctta
     2401 cttatttgat tgctgtgcct ctggggatgc ttgctggccg gtatcaaaat tcaatcttgg
     2461 ataagattgt cgtaatctac aacttcttta gttttgctgt tcctttattt atcttcggct
     2521 tgatcatgct atttgttttt ggttatcgtt taggctggtt cccaacaagt ggatcacaaa
     2581 cactaggttt gcaaggtgga tttatcactc aatggataga ccgtttgaaa tatattctgt
     2641 taccatcaat tacccaagca tttttggcga ctgccgtcac aatccaatat ctgcgaagtg
     2701 aagtaatcga ttcgaaatcg ctcgattttg tcagaacagc ccgttccaaa ggggtaccga
     2761 cgaataaagt attcagtcga catattttcc gaaatgcagc gttgccgatg gcttctcaaa
     2821 tggggtatga aatcacttcg ttgattgctg gttctgtagt tatcgaaaag atttttggtt
     2881 atccgggagt cggaaaacta tttattgatt ccatcggcca acgggactat accgtgatca
     2941 catccttagt attgatttta ggaatcgcga cgctagtggg gacattactt tcagatatca
     3001 tcatgagtat cgttgacccg cgtattcgga ttcaataaaa ccaatcagaa aggagaatgg
     3061 atcgatgagt gaaaaaaaca atacagcagt tcaagaaagt attcctccta tggggatacg
     3121 aatgattgct cgagaattta aaaaagataa aattgctatt ttttcattag gtttgctagt
     3181 ggttttatta ttagtgatct ttatcggtgc gatgctgacc gatcaagaca aagtgatgta
     3241 tgttagtatc ttcgacaaat acgctgcacc aggaacagtt tcgacacaag gacaaaagtt
     3301 cattctggga gcagatgaag gcggacgtga tgtattagga caattgatca taggtgctag
     3361 aaactctgtt acgatcggat ttgcgatcac gatccttact tcgatcatcg gggttggatt
     3421 agggatcatc tctggctact acggagggat cattgacaac atcttgatgc ggatcgttga
     3481 ttttatcatg attctaccga tcatgctgat cattatcgtc ttcgtatcga ttatttccag
     3541 atacaatgtc tggtccttta tattcatcat gagtgcattt tattgggtag ctaaagcccg
     3601 attattccga agcaagacat tatctgaagc aagaagagat tatgtcagtg cctcaaaaac
     3661 aatgggaacc aatgatttta aaatcatgtt ccgcgaaatc atgccgaatc taagttcatt
     3721 aatcattact aatttgacga tgaactttgc ggcaaacatc ggtatcgaga caactttgac
     3781 tttcttaggt ttcggcttgc cggcaaatgt acctagttta ggtacattga tcggttatgc
     3841 aagtaacgga gaagtcttag tgaatcggca atggatctgg ttacccgcat caatccttat
     3901 tttagttttg atgttgagta tcaattatgt tggacaagca ttcaagcgtt ctgcagatgc
     3961 gcgccaacgg ttaggctagt atagaagcga agggggaaat aggaatgaag aaaacgattt
     4021 ttggatttgc agcgctgatg tctgctgtcg ttcttgcagc atgcgggggc ggaggtacag
     4081 atacctctgg aaacaactct ggaggcaacg gcaataacaa agaggaatta acttttccaa
     4141 ttgcaacaaa aaataatgat gatgcaatcg aagacggcga attagaagct gcggtcgcaa
     4201 cggacagtca gtttaaagga ttgttccagt gggagtttta ccaagattca tatgatgcag
     4261 cttttatggc tccttctcat gaaccgctat ttgcaaacga tgacgatttc agaatcacag
     4321 acgacggagc tgcaacgctt gatctagatg aagatacgaa aaaagcgaca atcaaagtaa
     4381 aagacaacgt caaatggtca gatggtgaag atgtaacggc ggacgatgtg attttccctt
     4441 acgaaatcat tgggaataaa gattacactg gtatccgtta tgacgacact ttccgtaaca
     4501 tcgtaggtat ggaagaatat catgatggaa gtgccgacac aatctccgga atcaaaaaaa
     4561 tagacgacaa aacagttgaa atcgaatata aagaagtaaa cccaagcatg cttcaatcgg
     4621 gtggtggtat ctggtcatat gcagcaccaa aacatacgtt agaaggtgta gcaatcaaag
     4681 atatggaatc aagcgatcaa gtacgtaaaa atccagtgac ttttggccct tattacatga
     4741 gtaatatcgt tgcaggagaa tcagtagaat acttgccaaa cgagcactac tggaatggta
     4801 cgcctaaatt gaaaaagatc acgatgaaat ctgtaccgat ggcaagtgca actgaagcat
     4861 tgaactctaa attatatgat atgattttcc aaatgccaac agatacttac gatacctata
     4921 aagaaattgc tggctataca aacttaggcc gtgaacaaac ttcttatact tatctaggat
     4981 tcaagttagg taaatggaat gcagatacaa agagtgtgga atacaatcca gattcaaaaa
     5041 tggctgataa atcattacgt caagcaatgg gctatgcatt agataataat gcagtaggtg
     5101 aacgtttcta tgcaggtttg agaagcaatg cgacttcatt gattcctcca gtattccagt
     5161 cataccatga tgatgagatg aaaggattca cacaagatct agacaaagcg aaaaaactat
     5221 tagatgatgc tggctacaaa gatgtggatg acgatggttt aagagaagac aaagatggaa
     5281 ataaactaac aatcaacttt gcttcgatgt ccggtgggga aacagcgcaa ccattggcag
     5341 attactatat ccaacaatgg aaagaaattg gtttggatgt ccaatacaca acaggtcgtc
     5401 tgatcgaatt caattctttc tatgataaat tagaaaacga tgatcctgaa attgatgttt
     5461 accaagctgc ttggaataca ggaactgatc caaatccaag tggactatgg ggaccaaatg
     5521 ctgcgttcaa ctacactcgt tatgaatcag aagaaaatac aaaattgatc aacgatatca
     5581 gttcaagcaa agcatttgat gaagagtacc aaaaagaagc atatcaaaaa tggcaacaat
     5641 atgcatttga agaagcattt gcgattccaa cgttgttccg taatgaagta ttgccagtca
     5701 acgaccgtgt caaagactgg gattggtctt atgatgtacc aaacccatgg gcaaatgttt
     5761 ctgttacagc agattcacgt tcataatcac taaaaatgac cgaaaaaggg cgatgtcctt
     5821 tttcggtttt ttgatatttt tctgtttatt tctctaatat tcaggtcaag gcgctgattt
     5881 catgacttct ccaataaacg gtgttcaaag tccagtttct cggtattaag tcaaatttat
     5941 gcaaaaatat ggaaagccat tttcacaaaa ttttttcttc atactcgaaa ctaaacgaat
     6001 ttttcacaac ttctcgtcaa tcactaggat tattaaagct ttacttacct tttgaccgaa
     6061 ccatttcaaa tttgacaaaa aaccagtata ataaggaagt atcgaaaaaa tatccataaa
     6121 aggaatgtgg aaaatggaca accagctgac aaaagaacta aaagaaaact ataacatcgt
     6181 ttttcacgat ctaaatttgc ttgaacaagc ttttacccat tcatcctatg tgaatgagca
     6241 tcgcaatctg caattatcgg ataatgagcg acttgaattt ttaggagatg ccgttttaga
     6301 actgatggtt tctcagtatt tattccgttt atatcctcat ttgccagaag gaaaattgac
     6361 aaaaacacga gctgcgatcg ttcgcgaaga cagcttgtcg aaatttgcca aagaatgtca
     6421 ctttgatcaa tatgttttgt tgggcaaggg agaagagaat tcaggaggac gtacgcgacc
     6481 agcattgctt tgcgacctat tcgaagcttt tttaggcgcg ttatatcttg accagggatt
     6541 cgacacgacc catgcattca tcgaaaaagt gatttttcct aaagtcaaag caggtgcttt
     6601 ttcacatgag atggatcata aaacaaaact acaagaagtc ttgcaaaaat caggtgatgt
     6661 atcaatcgaa taccgattga tcaatgaaga aggtccagct catgaacgag tattttggat
     6721 cgaagtttat gtagatgatc aattgattgg cactggacaa gggaaatcga aaaaattagc
     6781 agaacaagct gcggcggaaa atgcattggc agcactgtaa aagtaagtga gggaaagctt
     6841 tgtatttgaa acgaattgaa atagccggtt ttaaatcatt tgcagacaag acagtcattg
     6901 attttgaaaa tagcgtcaca gcagtggttg gaccaaatgg cagcggaaaa agcaatatta
     6961 cagaggctat ccgctgggta ttaggagaac aatctgctaa aagtctgcga ggcggaaaaa
     7021 tgccggatat catctttgcc ggttccgata cccgtaagca gctgaatatt gcagaagtca
     7081 cagtgatttt agacaatagt gatcattatc tgccattgga gtacaatgag atcagtgtaa
     7141 cccgccgtta tcgacggact ggggaaagcg agtttttcat caataaacaa agctgcagat
     7201 taaaagatat ccaagaattg ttcttagatt ctggattagg aaaagaatcg ttctcaatca
     7261 tttctcaagg aaaagtagaa gcgattttca gtagtaaacc ggaagaccgt cgtgggatct
     7321 ttgaagaagc agctggtgtc ttgaaataca agcagcggaa aaagaaagca gaacagaagc
     7381 tttttgaaac agaagacaac ctcagccgcg tccaagacat catccacgaa ttggaagaac
     7441 agttaactcc tttggccgcc cagagtgagg cagcaaagga gtttttacgg ctaaaagaaa
     7501 cactgactca aaccgatgtc tctttgatgg ttgctgaaat caagacagca aaaaaagatt
     7561 gggacaacaa acaagcacag cttgcgaaat tcaatctaga attaggcaaa ctatctgagt
     7621 cgatccaaga acaagaatcc attttggcga agcagcgtaa agaaaatgcg caagccgatc
     7681 gtctgatcga aaaaaatcag caagtattat tagatctttc ggaaaaactg aagcagacag
     7741 aaggacaaaa agatgttttg caggagcgga caaagcatac acaaaaaagc tcgcaagaat
     7801 atcaaacttc attagcagaa gcacaaaaaa aggttaaaca ttttgaaaag cttcaagaaa
     7861 gcttgatgaa agcagcagca gaaaaagaaa cagagatcca aaaagctgaa gcaaatctca
     7921 taaaaacaca gcaagaatta gaaaaatacc aaaaatcaac gaaagaatta ttggcagaat
     7981 taagagatca atatgttgat ttgatgcaag aacaagcagc tgtaggaaac gaactgaagt
     8041 atctagaacg tcaatatata caagaaacag caaaaagcaa gcagacgtta gccaagcaat
     8101 cggaagtaga agcatcagta gaccgattga tcttacaaaa agaagaattg acacaaaagc
     8161 aggcgcagct aaaatcatct ttaactgaaa caaaagagaa gttagaaatg atccaacaaa
     8221 acgggaaaaa gttccaagaa gcattagcaa aagaacagcc taaaatgtat caattaatga
     8281 atcaagtcca gcagctacgt gctcgtaaga aaagccttca agaaatccaa gaaaactatt
     8341 ttggttttta ccaaggggtt cgattggtct tgcagcataa acagcagctt tcgggtattg
     8401 tcggagcagt ggctgaactg atcgatgttc ctgcagattt cacgttagct atcgaagcag
     8461 ctttaggtgg ggcagcccag catgtcatcg ttgaaaatga aaaagatgct cggcaagcca
     8521 tcacgtattt gaaacagcaa agaggaggaa gagcaacatt ccttccactg acgacaatca
     8581 aaccaagaca attaccggct catatattga cacaagcagc agctgtagaa gggtttatag
     8641 ggattgccag tgaacaagtc agttatcccg atcaaatcca aactgtggtt caaaacctgc
     8701 taggaacgat ccttttagcc aaagacttaa ctagtgccaa tgcaatcgcg caaacgatcc
     8761 gctatcaata tcgagtggtt tccttagaag gtgatgtgat gaatgccgga ggatccatga
     8821 caggtggagc gaataaacga ggaaaccaag gcagtttgtt tgtacaaaat caagaattga
     8881 aacagttgac tagtgaattt gaagaagcag ataaacaact ccaagcacaa gaaaaaaaag
     8941 ttcaggagct gcaacaagaa acagctcgtt tagccgaaga acaagaagtt ttgcgtacac
     9001 gaggcgaaca attacgtttt gaagaacaag aagcgacaaa tcagctacaa aacatcataa
     9061 atgaacttga acgttttgaa aaagaaaagc aaatctcaac ttttgagaca cgtgaactgc
     9121 agcagtttat cgaagattac gaaaagcaaa cgaacgaatt gaaagacaaa caaacagatc
     9181 ttgaatcgca acgacaaaaa atcgacgaag aaatcaaatc gttaagtcaa gaaagcgacc
     9241 agatggaagc cagaagagca caagtacaaa gtcaaaaagc acaagaacaa gcagatcttg
     9301 ctgtcttgaa agagcagttt aatcatctgc agatccagct ccgcggtgcc cgagtccaaa
     9361 aagcagaggc aaccgaacgt caagaagcga tagaaaagca gttggcgaca ttgacagcag
     9421 acttttctga tcatgaagtc acagaagaaa gtttggaaaa acaaattaac gaattatctg
     9481 ctcagcgaga aacattaaaa gccgagttgg caaaagccaa agagcagcga gatagaaggc
     9541 aaaaggagat cgataagctt gaagcagtct tggcagagcg taatcgtgag caaaaagcac
     9601 gactgtcgga acaatccaaa ttagaagtcc aaaaagaccg agctgaaatg attttagata
     9661 atcatttgac ctatctgcag tcagaatatc agctgacatt tgagaaagct tcccaagact
     9721 atcaagaaac aaccgatatc gaagacagta gaacaaaggt ttcatcctta aaagaacaga
     9781 ttgaaaaact gggaccagta aacttaaatt caatcgaaca atacgaacaa gtcagtgaaa
     9841 gacatacatt cttagcaaca cagcgagatg atctgttagc agcgaaaaac caattgtttg
     9901 aaacgatgga tgagatggat gatgaagtgc ggacaagatt caaagaagta tttgaagcta
     9961 tccgtcaaga attcaaaatt gtctttccaa atatgtttgg cggcggtaaa gctgaattag
    10021 tactgacgga accaactgat ttgctgaaaa caggtattga gattgaagta caacctcctg
    10081 gcaaaaaact gcaaagtcta agtttgcttt ctggaggaga acgagcactt acagccatcg
    10141 cgttgctctt ttccattatc cgtgtctgtc ctgttccgtt ttgtatctta gatgaggtag
    10201 aagcagcgtt agatgaagcc aacgtgaaac gttttggtcg ttacttgagc gatttccaag
    10261 atgacacaca atttatcgta gtcacacacc gcaaaggaac gatggtcgca gcagatgtac
    10321 tgtacggagt gacgatgcag gaatccggtg tatcaaaaat cgtttctgta cggatggaag
    10381 atatcaatga agaggcgaaa atcaacgaaa cgaaagtgta ggaacgaaaa tgatcaaatt
    10441 agtagcaatc gatttggacg gtacattatt aaatgaacaa aagctgattt cagatgagaa
    10501 taaacaagca ttagcacaag caaaacagca aggggtcaag atcgttctat gtacaggacg
    10561 ccccttagct gcgatggcgc attatctgca agaattagga ctggtagatg aaggtgacta
    10621 cagcatcact ttcaacggcg gtttggtaca aaaaaatgat actggtgaaa tcatggaaaa
    10681 gaaagtgatg gaagtatcag atatccaacg cttatacaaa ctggctcagc aattggatct
    10741 gccactagat gttttgtctg acaatgtggt tttacagctg cctagtgccc caaggaagca
    10801 atccttgtac aatgtgttga ataagctgct gcaattccaa ccagcggata taagcgatat
    10861 tacagatgaa tttgtgctta ataaggcagt aatcgcttat gaccaagaag aattagatcc
    10921 taaaatcaaa gaaataccag cagaattcca tgaaatgtat gagatcatca aaacaagaag
    10981 tatgcttttg gaatttatgc caaaaggtgt tacaaaagct tatggtattt ctttattggc
    11041 aaaagatctt ggtttgaaac aagaagaaat catggcgatc ggtgatgagg aaaatgattt
    11101 accgatgatc caatatgctg gactaggcgt agcgatggag aatgctgtgc ctttcgtcaa
    11161 agaagcagca gatttcgtga cagccagcaa tgaagaaaat ggcgtagcca aagtgatcca
    11221 aaaatttgta ttagagtagg gggagtcaaa tgggattttt tgataaaatc aagcgtgctt
    11281 ttactggcga agagaaaaca gaagaagttg aagtacagga gaagtacgac aagggtttag
    11341 aaaagacgcg taaaacattt ggtgaacgga tgaatgagtt gtttgccaat tttcgaacag
    11401 tcgatgaaga cttttttgaa gaattagaag aaacacttat cggtgcagat gtaggttttg
    11461 aaacagcgat acgaatcacc gaagcattac gtcaagaagt gaaactcaga aatgcgaaaa
    11521 aatcggcaga agtccaaaat gcgatcatcg agaaacttgt ggatctctat gaagcggaag
    11581 gaatcaatga agtaaatgaa ctaaatatcc aagaagacgg actaacagtt attttgtttg
    11641 tcggtgttaa tggtgtaggg aaaacaacaa gtattggtaa acttgcccat cagttcaaaa
    11701 acgaaggtaa aaaagtattg atggcagcag cagatacctt ccgcgctgga gcaatcgacc
    11761 agttagttgt ctggggcgaa cgcgctggtg ttgaagttgt tcgtggcaat gcaggaggcg
    11821 atccagcagc tgtcgtattc gatgctttgg aaagagcaaa gacagagcag gcagatgttt
    11881 tactagttga tactgcaggc cgactgcaaa ataaagtgaa cttgatgaac gaattagaaa
    11941 aaataaaacg agtaatccag cgggaattac cagaagcacc ccaagaaaca cttcttgtat
    12001 tagatgcaac cactggtcaa aacgcgatgg ttcaagcaaa acaattcaaa gaaacaacag
    12061 atgtgaccgg tcttgttttg acaaaattag atggtacagc aaagggcggt atcgtacttg
    12121 ctatccgtaa tgaattgcat ttaccagtga aattggtcgg tcttggtgaa ggaatcgatg
    12181 acttggagcc gtttgatcca aatgattttg tcgttggatt gttcaaagga ttactaaaag
    12241 aagaataacc catgtctaaa aagcaggagc tgtgacaaaa gttgcagctc cttgtttgaa
    12301 tgattgtttt taacatagct gtgtttttga tgccttcatt tttatactta tttgtaagcg
    12361 gttctcaaac aatttcgttc tttttcttag actttttgat tataattaga ttcatatgaa
    12421 ttggaggtca cttaatgatg aattttaagc aattattatt gcatgtcaat gcgcgccctt
    12481 ttatcgacca agctcgattt ggtatagaac gggaaggaca gcgggttgat cttgcaggaa
    12541 atctagcaaa aaccgatcat ccagcaatct ttggcgatcg atcctatcac ccctatatcc
    12601 aaacagattt tagcgaaaca caaacagaga tgatcacccc tgttaccgat tctattcccg
    12661 aattatttca gtatctcgct gctgtttatg atgtgactgc tcgttctata ccgaaagaag
    12721 agatgatctg gccattaagt atgccacctg ccttaccgga aaaagacgaa gagatcatta
    12781 ttgcaaaatt aaaaaatttc gaagatgtct tgtatcgacg ttatttagca aaaaaatacg
    12841 ggaagcgaaa acagatggta agcggtattc atttcaattt tgaatttggc gatgagttgc
    12901 taagaacatt gttcagccat caggaagaat ttcaagattt ttccgaattc aaaacagaac
    12961 tttatttgaa aacagcgaga aactttatgc gctatcgctg gatgatcact tatttattcg
    13021 gtgcttcacc aatgagtgag aagaactact ttttagatga atcacatccg caagaacctg
    13081 ttcgcagtat tcgcaacagt gcgctgggct atacgaatca tccaaatgtg aaagtttcct
    13141 atgcttctat gaaacagtat ttagcagata tcgagcgaat gatcgaagaa gggaaacttt
    13201 cggaggagaa agaattttat acaccgcttc gtttccgagg cggaaagaaa gtcgcagatt
    13261 tagcaacaac aggtgttcgt tatattgaat tgcggaatat cgatttgaat ccttatgcaa
    13321 gattgggtat caatccagag caagttcggt tcttacaatt attcctgatg tacatgttat
    13381 ggacagaaga aaaagaagac tgtgaccaat gggtagcaga aggtacgact cgaaataaca
    13441 aagtagcact agaacaacca tcagatcaaa cagaatttca tcaagaaggt agagaaattc
    13501 ttgaaggaat gaagcaaatg ctggttgaat tggattggtt ggattctctt tatttagtag
    13561 aggaagcatt gactcaaatg gatcatcctg aacaaacgtt agcagcgaaa ctctatcaag
    13621 aagcacagct atcgagtcag caagaagttg ctgtggcatt gggacatcaa tattataaag
    13681 aaagccatga acgtccctat cagttggccg gttttcgcga aatggaactt tctactcaga
    13741 tatttatgtt cgatgccatc caaaaaggtg tccaagtcaa agtattagat gagtcggatc
    13801 aatttttgcg tctgcaattc caagaccatg tagaatatgt gaaaaatgca aacatgacaa
    13861 gtaaggacag ctatatcgtc ccacttatca tggaaaacaa aacagtaacg aaaaaagtct
    13921 taaaagaagc tgggttccgg gtaccaggag gcgcagaatt ttcatccatg gaagaagcgg
    13981 taaaagctta tccgagattt gccgagcagg catttgtgat caaacctaaa tcaaccaatt
    14041 acgggttagg tatcacgatt ttcaaggaag gagctagtct ggaagattat caagcaggtt
    14101 tagccattgc ttttcgtgaa gacagttcgg ttttagtaga agaatttatg ccgggaacgg
    14161 aatatcgttt ctttgtgatc gacggagaag tccaagccat catgttgcga gtccctgcta
    14221 atgttattgg tgacagtatc cgaacagtga aagaacttgt tgaagaaaaa aatagtgacc
    14281 ctttgcgtgg aacgaatcat cgtgcacctt tagaattgat ccaattaggt gaattagaac
    14341 aattgatgtt gaaagaacaa gggttgacga tcgaatcagt tccccaagcc aatcaaattg
    14401 tttatctaag ggagaactca aatatcagta caggtggtga ttcgatcgat atgactgatg
    14461 aattttctga agcttacaaa aaaatcgccg tttctgcggt agaagcacta ggagctaaga
    14521 ttagtggtat cgatttgatt ataccagata aagaaatcga tcctacaact gataaaaaag
    14581 catatgggat catcgaagca aatttcaatc cagccatgca tatgcatgtt tatccttttg
    14641 caggaaaagg cagaagattg acgatgaatg tattgaaatt gttatatcct gaagtatttt
    14701 aaaagaaaaa gcttttgaca gattgtccta tttgatgtca atctgatcaa aagctttttt
    14761 tgcatgtaaa aatttatgtg ttttttcgtt ttaaaataaa cgatcccaga agcttttttt
    14821 cttttttggt ttttcttttt cagctggcga agagttttct tctgggtatt ttttttcgat
    14881 atcaatcaca ggttcattta ctgcttcttt tgctgttaag agcaatccga attcctcagg
    14941 atttgcagtg acgtaatcgt tgactacagt gaaacggcta ttgtttttcg ttgctagctg
    15001 gatatattgg ttttggacag aaaggggtag agccccattc aagagtatgg aagcatctgg
    15061 atgttttttg agttctttta aaaagttttc tttgtttttt ggttggatca tttcagcaac
    15121 agtcatacta agatagcagc gttccctgaa tgtccctaaa tatttatgct gttcttcagg
    15181 gttcactaaa ggtgtaccat aaagtccctt atccaaatgg ctttttacat catcttgtgc
    15241 cataaaaaat accctccttc ttctctttgt ctttcttacg attatagcat attcccaata
    15301 atacctaaaa catatgaaaa agcggttgct tttgttcaac gattcactta aaataagaat
    15361 gaaaatcatt ctcaattaga aaggtggatt ggatgcgcta cggacgacta gtagcattat
    15421 tagttatttt aagtagtatt tcattgttca tcggagtatc gaatgtacaa ttgtcagaac
    15481 tgctccagtt agatgggcaa agcttacaaa tcttgctagt gactcgtttg ccacgaacga
    15541 tttgtttgat ccttgtggga gcgaccagta gtatttgtgg gttgatcatg caacatttga
    15601 cccaaaataa atttgtttca ccaacgactg cgggcacgat ggatagtgcg cgtttaggta
    15661 ttttagtggc tatgattttc ctacctggtg catccttgat tgtccgatcc ttgacagcat
    15721 tttgctttgc ttttgcagga acattgcttt ttttatccct tacgcgattg ttcctccaga
    15781 aaaatcaatt gatgcttccg ttgattggag tcatgttagg gaatatcatt ggttcagttg
    15841 ctactttttt tgcttatcaa ttccagttgg tacaaaatat gacttcatgg cttcaaggga
    15901 attttgcgac tgtgatacga ggaaattatg agttattgta tgctgttgtt ccattgttgc
    15961 tcttggtttg gctatttgct taccgattta cgatcattgg tctgggcgaa tcgtttgcag
    16021 taaatctggg aatagattac cagaagatgc agtttttagg cattggctta gtatcattgg
    16081 ctagtgcagt catgttgatc atggtaggaa gcattccttt tatcggagtg atcattccga
    16141 atcttgtctc tagaatatat ggagatcaag tgcacaaaac aattggcgta actgctttag
    16201 caggtagttt gttcttagta ttttgcgatc ttgtcgctcg cgtgttgatt tttccttatg
    16261 aaataccagt cagtgtgatc gttggaatca tcggtggggc tatcttcctt tatcttctga
    16321 taaggggacg acgatatgct taaaaaaaag ccatctattt ttattggtat gcttgttctt
    16381 gttttatttg ttacaggaat gtacttgacg atccatacat atggaaactg gcattttgca
    16441 tgtgcattga ggagtaagaa aatttttgca tttatccttg tctcgctagc tgtcagcagt
    16501 gccacaatca gctttcagac aattacgcat aaccagtttt tgactccttc agtattggga
    16561 cttgagtcgt tatatgtcct tgtccaaacg gtccttttct tctttatcgg aggagtgacg
    16621 atgcttcagc agacaggtac agtttttttc ttattgaacg ttgggctgat ggtcgggctg
    16681 agtctattgt tatttttctt tcttttgaaa aaaatgagag cgaatctttt tttattactg
    16741 atgtcaggga tgattttagg tacgcttttt ggtagtgtca gtaccttttt acaagtcatc
    16801 atggatccga atgaatatga tttgcttcaa ggcagattat ttgcaagctt tggcaatatc
    16861 cagagtcagt atcttttgcc tgcttctttg atcattatcg gctgctggta cctgttattt
    16921 cgatatcgac atgtgctaga cgttttccat ctcggaaatg aacaagcagt aaacctcggg
    16981 atagacacag atcgttttga atttttgatt ttatgttgtg tcagtctttt gacagggaca
    17041 tcaacggctc ttgtgggtcc agttactttt ttagggttta ttgtcgcaaa tatcagttat
    17101 cgtgtctttt ctacttatcg tcatggtcag ctatttatcg gaagctttct ggtagctatg
    17161 ctgtttctga caggaggaca gctcgcagta gagcagattt tcggctggaa tacaacagtg
    17221 agtgtcgtcg ttgaatttgt cggaggaatt tattttgtca caaaattgat tcgcgaaagg
    17281 aagagtatcc tatgattgat ttacaaaagg tcaataaact gtaccatgga aaatcagtgg
    17341 ttaaagatgt acagttaacg attcctgaaa agaagttgac tgcttttatc ggtccaaatg
    17401 gtgctggaaa gtcaacggtg ttgtcgatga tcagtcgttt gataccaaaa gatacaggtg
    17461 aaatctattt agaccataac gaagtaaagg cttggaaatc caatgaatta gccaaaaaat
    17521 tatcgatcct acgacaatcg aatgaaataa atgtccaaat caccgtgaaa gaattagtag
    17581 aatttggacg atttccttat acgaaaggaa aattgacgac aaaagacgag gaaatcgttc
    17641 aggaatcact ggaacacctt gggttagaag aaatagcgga tcaatcgatt gacactcttt
    17701 caggcggtca actccaacgt gcgtatattg caatggtgct agcgcaagac acggattata
    17761 ttttactgga tgaacctttg aacaatctag atatgaattt tgcggttcaa atcatgcaga
    17821 ttttaaaaaa tttagtacaa gaaatcggga aaacaattgt tattgttctt catgatatca
    17881 actttgctgc aagttatgca gacgaaattg ttgcaatgaa agatggacag ttatttacgc
    17941 aaggaacgac ccaagaaatg ataaaaaaag atgtattgga tgaattatac gaaatggaca
    18001 tacggatttg tgagattgaa ggccgcagat tctgtcttta tttctaaaac taacattagg
    18061 agagaagaga tgaaaaaatt agtagtaggt atcttgattc tagcatcatt tggtttagca
    18121 gcatgtggaa atagcaacaa caccagtcag gcagacacaa aaaaaagcag cacacaaact
    18181 gaaactacat tgacgataac agatagtaat ggagaccaaa tcgaggtgcc aaataatcca
    18241 gaaaaagtcg tcgtattcga caatggttct ttagatacga tggatgctct tggtgttgga
    18301 gataaggtag ttggagcagc tacaagcagc cttcctgagt atctttcatc ctataaaaaa
    18361 gtagaatcag caggtggaat aaaagaacca gacttagaaa aaattaatca gcttcagcca
    18421 gatctaatca tcatttctgg ccgacaaaga gattttcaaa aagatctaag tcaaattgct
    18481 ccgaccattt tcctttcttt agatgcgaaa aatccatggg aatctttcca gcaaaatgtc
    18541 actgctttag gggagatctt tggtaagcaa gaagaagcta aaactcaatt agaggaatta
    18601 tctagtgcaa tcgatcagac gaaaaagaaa gcagaagcta ctgacaaaaa agcactagtc
    18661 acacttgtga atgaagggca attatcagcc tacggatctg gttctagatt cggtttcatc
    18721 catgatttat ttggctttga acaagcagat gatcaaatcg aagcatccac tcacggtcaa
    18781 agtgtttctt atgaatatgt gctggaaaaa aatccggata tcttatttgt agtagacaga
    18841 acaaaagcta ttggtggaga cgacagcaaa gatgatattt ctgccaatga actggtagca
    18901 caaacgaatg ctggtaaaaa tcagcagatc atctcccttg aaccagatgt ctggtattta
    18961 agcgggggcg gtttagaatc aatgaaactg atgatcgaag atgtgaatca agcatttaaa
    19021 tagcataaat aacagaggat taaactctca tactatgtaa caataatgtt tcttacattc
    19081 ttaaatagca agagcctatc ctcaaagaag ttatcaaaaa ccatccgtaa aatcgtattg
    19141 atgattttac ggatggtttt tatttatttg agaaatcttt tttcaacgtg aattcattat
    19201 ctacatcata cgggttgatt ctagaatggg gagtggcaaa aaatagtgtg atccattttt
    19261 tcagcatcaa gatcgcctcc tttgatttga atcaaaggta caaagcgaaa cttagataaa
    19321 acttaaattt agatattttt attttgtacc gatctcttct ttaaacgatg ttccatctgc
    19381 tgcttctcgg caataagtcc actagactag aaaatatgag aagctatttt ctgtccagcg
    19441 ttcttattac tcaaaacaaa tcgcagttgt cacaatctct tctaaacgat gttccatctg
    19501 ctgcttctcg gcaataagcc cactagacta gaaaatatga gaagctattt tctgtccagc
    19561 gttcttatta ctcaaagcaa atcgcagttg tcacaatctc ttctaaacaa tgttcaatca
    19621 gctgaccttc ggtattagct caaagcactg atttcacaac ctctatttag gcatgtgtct
    19681 cagcaagcgg ttgatagttg atgatctgca tttttcgata aaaatcggcg ggaagcttac
    19741 tccaccgttt tagtaattga tagaaataac tctcttctgc tttgatttta tactcgctta
    19801 atatcaatcc aaaactattc gttttttttg aagctaattt tttataaata tccaatgctt
    19861 tggttttatc ttcattgaca aagagtgttt ttacttcaat ataagaaccc tcgatatttt
    19921 gtgtttcttc gataaaatag atgtttttta tatattcctt catagtgatt cctccaaaga
    19981 agtcttttat ttgataagtt tactctaccg aagagttgtt aataatccgt gaacaaaaag
    20041 taattctttt tccaatagtt gttaaaggtt ttttattttt tatagtattt tttgttattt
    20101 aagaaaggaa taaggttaaa caaataacag gttgcttttt gggtcttttt ccgttcaaga
    20161 aactgaacga agaaaaggga aagttgtctc ttttgtgata agagaaatca taataaacga
    20221 atgacttact ttccaataaa ttgagaacag tcagaaccag acaaattagt tgcatctgat
    20281 gaataaagtg attattctat gacatgaggt gatacagatg aaattagaag tgacaaaaga
    20341 agcacaagaa gtattgaaga ataaattaga agcagacgat caacttttat tagatatcga
    20401 aaacggtgac ggtccatttg cagaaagcca agtcagttgc caattggata cggcatttcg
    20461 attgatcatt gtcaaaaaag atacacaaac agatctaagt ctttattctg tagtggctga
    20521 tacaccagtt ggaccaatca aaatcaaaga cagtgcaata ctttacatgg atgatccatc
    20581 cacacttgct ttagaaccaa cttataatag tttacaatta aaaggaccaa gcggcttgag
    20641 aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat aaaatgggaa aattggcgat
    20701 catcagcgac ctacatgtgg acatcaataa attcggcgaa tcagagctga tgcagctttg
    20761 gcaagttttg caaaagcaca agatcactcg gcttcatcta gccggagata cggctaataa
    20821 agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat aaaggattat ctacgacatt
    20881 tcatttcggc aatcacgagt tggcagatgt gcccaatgaa cagatgatgg accattttcc
    20941 agacccacat tttttaaatg aacggtatat tgctttaaat gagcagaccg ttcttttagg
    21001 gatgaatggc tggtacgact atcagttttc cgaattgaaa gattacgagg caatcgtacg
    21061 aatgaagcgt ttgtattggt atgatcggtt catccagcgt gggaaaacag atctcgaggt
    21121 caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac cagctggaag caaaaaaact
    21181 tgaggtgatt ttatccactc atttcgttcc taaacgggca tttatcatgt atcaatctgc
    21241 tccttatgag cgttggaaca aattaaatgc atttttagga tcagaatcat tcggagaatt
    21301 acttgaccgt tattcaaata tcaaacaagt agtttttggc catacgcatc gtcgctttga
    21361 agataaatcc atcggtcaca cgatttacag ctgcagacct tttggttatt attatgaatg
    21421 gcagctgacc cgagattttg tcttgaaaga acagttgcta gacagctatg atcctatgaa
    21481 attacgtgtc ctcttgcgtg cccatcaccc agcttttcag gcctatcaaa cacaacattt
    21541 gcaagaggaa tttgaacaag cgatgacaat cgttccgtat tgagacagca ttagtaatgt
    21601 gtaaaagaaa ataccttgta aaacataagg ataagtaaga gcagtagatc catttatcgt
    21661 ggatatgctg ctttttttgc ggaaaataat cagcgaatgt ttatttgatc ggatgatttg
    21721 taactcgcag agtactagat gcaagcgtgt attctttttt tgaacgataa tcacaaaaaa
    21781 aaatataata tacatttttt atttaattat aattaatatt gattgaagtc taggcagtca
    21841 agtcacattt tgatagatag gagggggact tacgaattat tttaaaggct atgacagctg
    21901 tatttctaga ttgtgtttgc atcaagttgc aataaaaact agaaatcaaa tcgggctgat
    21961 aatcatttat aactaatcga tacataggac caaatatagg tctatgttct tttatttcat
    22021 aagtatcgta aaacagaatt ttgctatgga ttaatccaat cgactgatat gtttagtaaa
    22081 taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta ttaacagcag caatgatgtt
    22141 attcagcatg gtagcaagta cgacttttgt ttctgcaact gaagtatacc caaaggaata
    22201 taacacagaa ggaacgatta catttgaagc aggagatgag ggtgtaactc ctcctgttga
    22261 tccagagaat cctgatccca ataaacctgt cgatccatca gatcctccaa gccctggaac
    22321 aggaggggca ttatcgattg attatggatc aaaattcaaa tttgggactc aaaaaatttc
    22381 tactgcggat aaaacgtatt atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc
    22441 gacatatgtc caagtgacag ataggagaag taccttatct gggtggaaat taagtgtctc
    22501 tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta gttggagcac agctgaaatt
    22561 tacgaaaggg caggctgttt ctttggttga tccaacatat acgccgcaaa cggtgaattc
    22621 tgaattaacg ttgacaccgg gaggaaacaa tacgttagca attaatgcta aatcaggaac
    22681 aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt
    22741 acagctttcg gtacccggta aatctgtcaa gttagcgcaa caatattcca ctaaattagt
    22801 ttggacatta gaagatacac ctaacaacta gtttactgaa aagaactgtt aaaaataaat
    22861 acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt
    22921 tgtttggata cgaatagttc atctaggaat ttaacgatca ggaggaaaag atatattgaa
    22981 aaagaggagg atcgtgctca ttttaagcag tctcttagtg actaatatct ttccgatcat
    23041 agggtactct ttggaattga atcagttgga gcatggaaat agaagagacg aagaaagcaa
    23101 actcacttcg acattcgaca gtttaataga atccaaccag acacaagaaa gcagtgagga
    23161 tttaagtatt tcgagtagtg gagagaccac tgacaacgta gaagctgagg gttcggacaa
    23221 acaaggacta gaagatgcac caatttctgc ggttgagctg cctgacgatc ccaatattat
    23281 ttctatcgat aaaatttttc aaacacctat cggtactagc acgtccatat tggaaaatgg
    23341 gaagttactg cagttgaacc cagcagaaag gtctcaaaga ggagctattt ggtcaaataa
    23401 gaagattagt ttactctcgg attttacatt taaatcttat ttatacttag gaaatcaacg
    23461 agatagagcg ggagatggca tgacctttac attgactggc gatcctagaa tggctagtaa
    23521 tcccagtcaa gttattggat cgccaggtat gggaattggc tcatattcta ctcgttctgg
    23581 ccaaccttac attgcgaatg cgctctcgat tgagtttgat acgtataaaa atagtggttc
    23641 ttctgatcga atggatcgag agataactgc tgataataga gggcatggac atgtcgcttt
    23701 tgtcacgcct aaaccaaata ataataatta ttctggagag catactggcg tgacggtagc
    23761 tcctacattt ctttctaatg gcacatggcg cttgttaata attaaatggg acgcggagca
    23821 aagaagtctt acttatgatt tggaaggtgt cggtcgctct tctcggatta tacctgatct
    23881 cattaggcag tttggttcta cagaagtaaa ttgggggttt acttcttcaa caggtacttt
    23941 ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg aatgtcacat caacagctga
    24001 tgtaaaagtc aacgatagtg attatggggc catcgctgaa gctggaggaa acgataagat
    24061 aactatcaga aatacattga aggtcgaggg ggattttctt gaatataaga aaatgcagac
    24121 tagtatcgac ctcccacaag aattaactat tcccgagcat atcttgatca atggaaatca
    24181 aattgatgcc aaagatatct tgataaccgg aaatcgagtc acgctaaatc tgacagatta
    24241 ttttattgct ggagatacgt taataatcga attggagaca actttgcagg aagtaattcc
    24301 tgaaaaagta ctcactatgc attttgaata ccttgaaaac gggaatttga ttgagcaatc
    24361 gaatgaaata aacctaaaga tcgcaaagct gaaggagata gatatccatg tttattataa
    24421 agataaagac agtcagattg atattgctga aatgaaaaca atcactggga aaataggaga
    24481 gaagtataaa gaaactccta ttgatattga agggtatgtt tttgactcgg attcagcgaa
    24541 tacagaaggc atcttctccg aaaatacaga ggatatctat ttttattaca gaaaaggcaa
    24601 actttatctc tcaactgctc ctcaatatgt caattttgga aaacataaaa tatccagtag
    24661 accattaagt gaattcggac agttttcagg tgggttgaaa attatagatg aacgggcttt
    24721 tggaagttgg aaattacaat tgaaacaaga gaaagttctt acaaatggag attttgagct
    24781 tccagaagct ctttcctttg ttacctctga aaatacaacg gttataggga catccgctgt
    24841 tacgattttc gaaagtgatc aaaagggtga aagcgatttg tcctctcttt tagattctac
    24901 agctcatcgt ggaatcagaa taaatattcc tgtagaaaac caaagaatcg gaacctttga
    24961 ggggaaacta tcttggatac ttgcagatgt cccaggaaat taacaggaag agaagaatat
    25021 aacaaaatta ttgagtttca agaagggcac aggtaccata ggctcttgtg tcccacagag
    25081 acaagatttt gttatagtct tctcttacat atgattaaag gagcgatttt cttgagacca
    25141 aaacaacaat tatttataac actcctagtt attttagggg cactttttaa caatatatca
    25201 gtgtcagcta gtgcatttaa tttttcagtg acgccgataa cttcagagaa tcaaatcgac
    25261 aagagaaaaa cttacttcga tcttcaactt gatccggatc aggaagtaga agtaaaagcg
    25321 gaattgagaa atgatacaga aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc
    25381 accaattcaa atgtgatggt tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg
    25441 atctttgatc tggtagatta tgtgagctat ccacagatag tgacgctaaa acctaaaagt
    25501 gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc gatttgatgg tgtattggct
    25561 ggtgggatca cattccaaga aatccagacg gaaaaggacc aaacagaaac taaggatcaa
    25621 agcttatcga ttgagaatga atacacctat attattgctt tgttgatgca acagacactg
    25681 aacgaagttg ctccgaacct tactcttcat gaagttaaac cagatcaaat caatgcaaga
    25741 aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct atatcaatca agtagttatt
    25801 gaaacaaaaa ttacaaaaaa aggacattct gaagttttat atcaagagga aaaagaagga
    25861 ttgcagattg cgccgaatac aaatttttct tttccaactg ctttaaatgg tcaaccgcta
    25921 accccaggtg aatatcacct tacaatgact gttttaggaa atgaaaatga aaatggaaaa
    25981 ttttcgagaa aaaaaggaaa tactacgatc aactatacca atcaatgggt atttgaaaaa
    26041 gattttacaa tagacggaaa agtagccaag gaattgaaca cgacggatgt tacgattaaa
    26101 aaagacaata attggattta tatcttagta gcttcacttt tcttattgtt tattttatta
    26161 tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag aaaagtaagg ttaagataac
    26221 aaatagtaaa tttttattat tgtaggatgg ttgggctctt tttataacaa tataaaatat
    26281 accattttat taataaaaat gcatactagt aaatgatata attctaataa caggtttgat
    26341 cgcgagaaag gccaaataaa acgctctcca aaatgttaaa aaaaagacag gacgatgtat
    26401 attatacaaa caagaatcgc tgacaacagt gactcggatc caggaaatca gttttatccc
    26461 cagctagttg gtgaatgcat ggaaagtaat atacgtagaa agagctgatg actaatgaat
    26521 tatgagtttt ttttaagcaa agaagatcac agaagttatt cattacttaa atatttagaa
    26581 gcaagctcaa atttatcaga gtcaatatcg aatgttcaag aagagttgtc gctatcgaca
    26641 tttctcttga aaaaaacgat cgataagctg aaacatgatt tacaaaagtt cgatctagag
    26701 aaaaatttgc gagtaaccgt cagtgaattg gatatatgtc tggaaattaa tggaaaatgt
    26761 tctagcaaag ctctgttatc tcgttatata acggattctt tatctatgaa aatggtattg
    26821 gctttctttc aggaaaaata tatctcagta gaaaattttg ctaaacagca tcacgtgagt
    26881 tattctgtgg cttataaagt attacaaaga ctaaaaagaa atctaaaaaa atatcaaatt
    26941 ttttttgaaa agagaaagtt ga
//
LOCUS       DAYYAJ010000026        26618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000026 DAYYAJ010000000
VERSION     DAYYAJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.026"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            547..1275
                     /locus_tag="ACHATZ_000944"
     CDS             547..1275
                     /locus_tag="ACHATZ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8403492.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            1293..2486
                     /locus_tag="ACHATZ_000945"
     CDS             1293..2486
                     /locus_tag="ACHATZ_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8403493.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            2479..3474
                     /gene="lacD"
                     /locus_tag="ACHATZ_000946"
     CDS             2479..3474
                     /gene="lacD"
                     /locus_tag="ACHATZ_000946"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8403494.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            3471..4412
                     /locus_tag="ACHATZ_000947"
     CDS             3471..4412
                     /locus_tag="ACHATZ_000947"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8403495.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            4458..6224
                     /locus_tag="ACHATZ_000948"
     CDS             4458..6224
                     /locus_tag="ACHATZ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM8403496.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            6241..6720
                     /locus_tag="ACHATZ_000949"
     CDS             6241..6720
                     /locus_tag="ACHATZ_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8403497.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            6733..7623
                     /locus_tag="ACHATZ_000950"
     CDS             6733..7623
                     /locus_tag="ACHATZ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8403498.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            7610..8419
                     /locus_tag="ACHATZ_000951"
     CDS             7610..8419
                     /locus_tag="ACHATZ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8403499.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            8435..8839
                     /locus_tag="ACHATZ_000952"
     CDS             8435..8839
                     /locus_tag="ACHATZ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8403500.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(9020..9958)
                     /gene="yidC"
                     /locus_tag="ACHATZ_000953"
     CDS             complement(9020..9958)
                     /gene="yidC"
                     /locus_tag="ACHATZ_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8403501.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(10292..11374)
                     /locus_tag="ACHATZ_000954"
     CDS             complement(10292..11374)
                     /locus_tag="ACHATZ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8403502.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11516..12967
                     /locus_tag="ACHATZ_000955"
     CDS             11516..12967
                     /locus_tag="ACHATZ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8403503.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            12960..13682
                     /locus_tag="ACHATZ_000956"
     CDS             12960..13682
                     /locus_tag="ACHATZ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8403504.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            13702..14853
                     /locus_tag="ACHATZ_000957"
     CDS             13702..14853
                     /locus_tag="ACHATZ_000957"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8403505.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(15201..15476)
                     /locus_tag="ACHATZ_000958"
     CDS             complement(15201..15476)
                     /locus_tag="ACHATZ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8403506.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            15584..16348
                     /locus_tag="ACHATZ_000959"
     CDS             15584..16348
                     /locus_tag="ACHATZ_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8403507.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            16472..16975
                     /locus_tag="ACHATZ_000960"
     CDS             16472..16975
                     /locus_tag="ACHATZ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8403508.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            17240..17887
                     /locus_tag="ACHATZ_000961"
     CDS             17240..17887
                     /locus_tag="ACHATZ_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8403509.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      17930..17997
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            18048..19316
                     /locus_tag="ACHATZ_000962"
     CDS             18048..19316
                     /locus_tag="ACHATZ_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8403510.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(19493..20695)
                     /locus_tag="ACHATZ_000963"
     CDS             complement(19493..20695)
                     /locus_tag="ACHATZ_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8403511.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            21222..21947
                     /locus_tag="ACHATZ_000964"
     CDS             21222..21947
                     /locus_tag="ACHATZ_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM8403512.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            21937..22611
                     /locus_tag="ACHATZ_000965"
     CDS             21937..22611
                     /locus_tag="ACHATZ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM8403513.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(22899..23183)
                     /locus_tag="ACHATZ_000966"
     CDS             complement(22899..23183)
                     /locus_tag="ACHATZ_000966"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM8403514.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(23477..24784)
                     /locus_tag="ACHATZ_000967"
     CDS             complement(23477..24784)
                     /locus_tag="ACHATZ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8403515.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            25273..26379
                     /locus_tag="ACHATZ_000968"
     CDS             25273..26379
                     /locus_tag="ACHATZ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403516.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         8570 a         4629 c         5570 g         7849 t
ORIGIN      
        1 taaagttcac aaacatcgaa aagtatagag cccgaatttt acgaatattt tttgtaatat
       61 aaaagtatag atactaaatt cttattcgtt aaacttacgc cataaggaaa ctagaaaaag
      121 acagaattat cataaaaaat ttttgttatt tttaaaaatg tacaacaaaa tttcgactat
      181 cagaccggca tgagagaaaa gttatctaaa aaataaaaaa tcctacttta tttagagaaa
      241 gccattttta tttacatagt agtctattag gcccatttgc ttgtcgatag tagaaatagc
      301 cacagatgta taaagaatat tttttcttta cttctctaga ccatttagct gaatctttgc
      361 caagattcag cttttttttg ttttttattt gtcaactggt atttttgagg tatcttttga
      421 taccagttct aaattacctt gaaaccttgt tataatagct tttttacgcc aaaaaccctt
      481 tgacaaatga ggaatatgtg ggtattcttg tgttgtggtt ataaccagta aagaagaggt
      541 atttttatgg agagctcaag taaaggaaaa tcattatatt atcaactagt tgacgtattg
      601 aaagaacgta tcgaaacaca gatgacacct catgacaaat tgttatctga aagagaactt
      661 tgtcagcatt atggagtgag tcggacaacg gttcgccttg ctttgcaaga attagagacg
      721 caagggtata tttatcgccg acacggaaaa ggaacatatg tgtctgatct atcagataag
      781 tcgtctgatc tggcgggagc gtatagtttt acagaagaga tgagggaatt gggacgggtg
      841 cctaatacag tcatcttgga attcaagcaa atggaagcaa acaaaaaggt tgccaagaat
      901 ctaaacatca atttaggcga aacagttttc aaattgaaaa gacttcgttt agcggatgac
      961 gaaccattga tggtggaacg ttcttattta cctgcaaaac tatttattgg tcttgatctg
     1021 ccattacttg aaagtaagcc attgtatgat gtgttttctc aggattatga acagcaagtc
     1081 caattagcag atgaagaatt ttatgcaagt atcgttcaaa agaaagatgc tgatccgctt
     1141 cgtatcgctg atggtgcccc ggtattatat ctaagccgaa cgacctataa tgtgaaaaat
     1201 gaaattattg aatatacgga tagtgtagcg agagcagacc agtttcatta taagatccgc
     1261 cacgtccgaa aatgattgag agggaaaaca acatgtttga actatcgaag gataaattaa
     1321 aagagttggg tgcggaaata actacgcgag aaatcaaaca gcaaccgcaa ttatggaaag
     1381 aaacagctga gatattagca cagaatcaag aaaaattagc attgtttttt gaccagataa
     1441 aaaaagcagc aaatggcaaa agagttcgag tgatttttac tggtgcaggc acatcacaat
     1501 atgtcgggga tacagcagta ccttatttaa atgaatacgg tgataacgat cactttattt
     1561 ttcatagctt tggtacgaca gatatcgtgt cttcaccaaa agaatttcta tttccggatg
     1621 aaccgacatt actggtgtca tttgcacgta gtggaaatag tccagaaagt gtggcaacag
     1681 tcaagattgc caatgaacaa atcaagacat tgtttcattt gacgattact tgtgctaaag
     1741 aaggtcagtt agcaaagaat gcagcgacag atgaacgaaa ttatctttta ctgatgccag
     1801 aaaaatcaaa tgatgcagga tttgccatga caggaagttt tacgtgcatg ttgttgagtg
     1861 cgttatgggt attcgaccaa actgccgttg agaaaaaaga aagttatctt caacaattga
     1921 tcagaactgg agaagatcta atttcacgag atcaagaaat ccaaaaatta ttgcctaagt
     1981 catttgaacg tatcgcctat cttggttcag gaagtttggc tggtgcaacg agagaagcac
     2041 aattgaaaat attggaattg acagccggta aagtggcaac gatctttgat tcttctatgg
     2101 gcttccgcca tggaccaaaa tcgtttttaa atagcgaaac acttgttatt ggttttgtta
     2161 ataaccaacc gtatacaaga aattatgact tagatattct agaagaaata aaagatgacc
     2221 aaattaccac gagtgtcatc gcgatcgccc aaccaggaaa catcgatttc acaggtacaa
     2281 gtcttttgtt agaagcttcg atcagcctac ctgatgcgta tcttgcctta cctatgatca
     2341 tgacagccca aatcattgcg ctcgatacgt cgataaaagt agcgaataaa ccagatacac
     2401 catcgcctac aggtacagtc aatcgagtgg tcaaaggtgt cattatccat gaatatgtga
     2461 acaaagggga aacgaaggat gaatgaagaa aaaattgcag ctatgaagag tttatcaaca
     2521 aaagaaggaa tcatcagtgc attggcaatt gatcaacgcg gtgctttgaa aaagatgatc
     2581 aaagcgttag gaaaagaacc aacggcgcaa cagatcactg aattcaaaga attagtatca
     2641 gcagaattaa cgccttatgc ttctgctatt ttactagatc cagagtatgg attgccagct
     2701 gctaaagcca gacatcagga agcaggacta cttttagcct atgaaaaaac gggttatgat
     2761 gcgacaactc caggacgtct gcctgattta ttagcggatt ggtctacgct tagattaaag
     2821 gaacaaacag ccgatgcgat caagtttttg ctttattatg atgtagatga agacccagag
     2881 atcaatcatt tgaaacatgt atttatcgaa cgtttaggca gtgaatgtgc aggtgaaaag
     2941 cgtccgtttt atctagaatt ggtttcgtat gatgccaaaa ccaatgacag cacttcatta
     3001 gaatatgcga agatcaaacc gcataaagtg atcgagatga tgaaagaatt ttctgatcct
     3061 aaatataaag tggatgtatt gaaagtagaa gtgccagtca atatgaacta tgtagaaggt
     3121 tattctgatg gtgaaattgc gtatagtaaa aaagaagcag cggcattctt caaggaacaa
     3181 agtgatgcaa caccgttacc atttatcttc ttgagtgcag gggtttcgac tgcattgttc
     3241 caacaaacat tgatctttgc tcatgaatct ggttcaacat tcaacggtgt gttatgcgga
     3301 cgtgcgactt ggaaagatgg agtcaaagca tatgttgaaa atggtagaga aggtgcgatt
     3361 gagtggttga gaacacaagg gcgacaaaat atcgaatcgt tgaataaagt gataaatgaa
     3421 aacgccaccc cttggtttga caaagtaaaa gaagattcgg tgacagcaaa atgatcacga
     3481 cagttaccat gaatccttca attgacatcg cttacacgtt ggatgaatta cggatcgacc
     3541 aagtcaatcg ggtcaaaacg gtcaacaaaa cggctggagg aaaaggcttg aatgttgctc
     3601 gtgtccttca tttattagca gatgatgttt tagctactgg attagtcggc gggcagcatg
     3661 gagcatatat caaagaacaa ctcgatcaat cagggataac gcatcaattt ttacctatca
     3721 aaggggaaac gagagattcg attgctattt tacatggagt gaatcaaaca gaactattgg
     3781 aaagcggtcc tgttgtgacg gaagaagagc aacataaatt taccgagcat tttgcacgac
     3841 taatggaaac aactgatatc tttacgattt cgggtagtct gcctcaagga ttttctaaaa
     3901 gttattatgc agagttactt gccctaacga ataaaaaagg aacacgagta ttgttagata
     3961 cttctggtca aaccttgctt gaagtcttgg aaggcaagga aaaaccattt ttgatcaaac
     4021 ctaatttgga agaattagga gagttggtcc accaaacaat ttcttcggct acttcgttag
     4081 atgaaatcca atcattattg gatcatccgc tttttgaagg aatcaattgg atcgttcttt
     4141 cgctaggcgc aaaaggagca atcgctaaag tagggagaca cttctatcag gttacgattc
     4201 ctaaaatcgc ggtggtgaat cctgtggggt caggagattc gactattgca ggcttagcac
     4261 atgctctatc aaagagctat acgattgaag aaacacttgc gtttgcgatg gcctgtggga
     4321 tggcgaatgc acaacaagaa aaaacaggat ggatcgatcc tgtgaaagtc gattatttta
     4381 ggggggaaat caacgtcaga aagattcgtt gattataaaa ttagaaagcg gtttctaaaa
     4441 acgaggagga aatttgcttg actagatttg aaataaaaga agagtttttg ttagacggtc
     4501 aacctttcaa aattttatct ggtgccatcc attatttccg agtgcatccg acgaattggg
     4561 agcggtcatt gcacaatttg aaagcgcttg gatttaatac tgtggaaacc tatgtgcctt
     4621 ggaatctcca tgaaaaagaa gaaggaacct atgatttttc gaaaatattg gatctttccc
     4681 attttctaga gttagcgcaa tcccttggat tatacgcaat cgtacgtcca gccccttata
     4741 tttgtgcaga atgggaattt ggtggcttgc ctgcttggct attgacaaag gagtgccgta
     4801 ttcgttcagc ggatcctcgc ttcatgaagt atgtcaaacg gtattaccaa acgctactac
     4861 ctaaaatcgt tcctcatcta gtcactcatg gtggcaacgt cttaatggtg caggtagaaa
     4921 atgagtacgg ttcgtatggt gaagagaaag catacctgcg ccaagtaaaa aaatacttgg
     4981 aggaaggtgg aatcgatgta ccgctattca cttctgatgg accttggcaa gccacattaa
     5041 gagcaggcat tttgattgac gatgatgttt ttacgacagg gaattttggc tcaaaagcaa
     5101 aagaaaattt tgcagatatg gcccagtttt ttgaagcaca tggtaaaaaa tggccgttga
     5161 tgtgtatgga attttgggat ggctggttta atcgttggaa agagccgatc attaaaagag
     5221 atccggaaga attagcggat gcagtcaaag aagcattaca gatcggcagt atcaatctct
     5281 atatgttcca tggcggaacc aattttgggt ttatgaatgg ctgttcagcc agaggcgcga
     5341 ttgatttgcc gcaagtcact tcctatgatt acgatgcacc attggatgag cagggaaatc
     5401 caacggaaaa atattttgct ctgaaacgaa tgatggcaga aaattttcca gaaatgcagc
     5461 aaatggaacc atggataaaa gaaacaatcg tcaaaaaagg aatcaacttg aaagaaaaag
     5521 tcagcttttt cgcaacactg gataccatca gcaagccgat ccactcgacc tatcctcaaa
     5581 caatggaaga attgggtcag aatactgggt acctattgta tcgaacaacg atcaaaaaag
     5641 atgcggtcaa tgaaaaatta cggatcatcg acggacgtga tcgggtccat gtttttctcg
     5701 atgaagaaaa gcaagcgatt caataccaaa cagagatcgg tgagaccatt ccattgacac
     5761 ttgagaaaga agaccatcaa attgatctat tatttgaaaa tatgggacgg gtcaactatg
     5821 gacacaagct gctagctgat acgcaaagaa aaggaatacg tacaggagtg atgagcgatc
     5881 ttcattttat cacagaatgg acacagtatt gtttaccttt agagactaca gaacacatcg
     5941 atttttcaaa aaaatgggaa gcaggtcaac cagcatttta ccgctttgaa gcggaactgt
     6001 cagaaatcgg tgatacttat ttggacctta gtgagtttgg gaaagggatc gtttgggtga
     6061 atggcacgaa tattggtcgt ttttgggaag taggaccaat tctttcactg tttgttcccg
     6121 agggtttatt tagaaaagga caaaatgaaa tcgttatctt tgaaacagaa ggtagattca
     6181 gtgaagttat agattttgtc aaagaaccaa tagaaaaaag ttagaaaagg agaatggatg
     6241 atggcaatta ttggagtaag aattgatgga cgtttgatac atggacaagt ggcaaatctt
     6301 tggacgacaa agttgaatat tggtcggatc atggtcgtag acgatgaggt agcaacaaat
     6361 gcgatcgaaa aaagtgggtt aaagttggca acgccagctg gagtcaaatt aagtatttta
     6421 cctattgaaa aagcagccgc aaatatttta gcaggtaaat atgattctca acgagtgctg
     6481 atcgtagcga aaaaaccaga tcgtatctta gcgctagtag aaaaaggtgt accgatcaaa
     6541 gaattaaatg tcggcaatat gtcacaaaca gatgaaacac gttccatcac aaaatcaatc
     6601 aatgtcgtag atgcggatgt agaggtcttc aagaaattag atcagcttgg cgttcatttg
     6661 atcgctcaaa tggtgccaaa tgacaaagca gaagacttta tgagtctttt aatcaaatag
     6721 gaggaatgat tcatggatat tcaatggtgg cagatattat tattaacatt atatgcaggg
     6781 tatcaaattt tagatgaatt acagatttat tcttcaatga gcgccccagt ttttgcagga
     6841 ttatatgctg ggttagtaat gggtgattta aaagcaggtt taatcattgg tggaagtatg
     6901 cagttgactg ttttaggggt agggacattt ggtggtgcat caaaaatcga tgccaactct
     6961 ggaacgatct tagcaacagc tttctccatt tctctaggga tgaacccaga acaagcgatt
     7021 gcggccatcg cggttcctgt agcaagtttg atgatccaat tagatatttt ggcacgtttt
     7081 gcgaatacgt attttgcaca tcggatcgat caacatgtgg aaacaatgaa ttacaaagca
     7141 attgaacgga acttcttgat gggggcatta ccttgggcac tttccagaat ggttcctgta
     7201 ttcttggccc tagcattcgg tggtggctta gtccaaaatg ttgtggctgt tttaaacggc
     7261 gacctaaaat ggctaggaga tggcttatct gttgcagggg cagtcttacc agcagttggg
     7321 tttgcaattt tactacgcta cctaccagta aagaaacaca tttcttatct gattttagga
     7381 tttacgatca cagcgttatt agcaacagtg ttctcgaatg tccaattgtt aggaacaagt
     7441 gtggctagcg tggtcaaaga tttcccagga acattcaatt cattaccgat gcttgctgtg
     7501 gcattgattg gtctagcact ggcaacgatc agttataaga atgatcaaaa gaatgaccaa
     7561 catccagtag taagtcagca atcacaatct gatgaggggg aaatcgaaga tgacgaaatc
     7621 taattataaa ttgaccaaag aagattttaa gcagatcaat cgtagaagtt tgtttacttt
     7681 ccaattaggt tggaactatg aaagaatgca aggttcaggt tatctgtata ctatcttgcc
     7741 acaacttaga aaaatctatg gtgatgatac accagaatta aaagaaatga tgaaaacaca
     7801 tacgcaattt tttaatacgt ctaatttctt taacacgatc attactggga tcgatcttgc
     7861 tatcgaagaa aaagaaggag tctcttcaaa ggaaactgtt tctgggatga aagcaggatt
     7921 gatgggttcc ttcgctgcaa ttggtgattc tgtatttgcg gcattgatcc cgaccatatt
     7981 tggagcttta gccgcaaata tggcaacaga tggtaatcca gtaggtatcc ttatctggat
     8041 ccttgctcag gtcgctgtca tggtctttcg ttggaaacaa ctagaatttg cctacaaaga
     8101 agggatttct ctagttacca cgatgcaatc acgcttgact gcattgacag atgcggctac
     8161 tttactagga gttttcatgg ttggagcgtt agtagcaacc atgatcaatg ttaaattctc
     8221 atgggcacca acgattgggg atgtaacgtt gaatatgcaa aactatatcg atatgatctt
     8281 acctaaactt ttaccagctg gaatcgttgg tggcgtttat tggatgttag gtaagaaaaa
     8341 tatgacacct actcgagcaa tattcatcgt attgatcgta tgtgtagcat tatctgcatt
     8401 aggtgtgatt tcgaaataag ggagaattgg aaagatgagt agacaactag tcttgattag
     8461 tcatggtaaa ttttgtgaag aactaaaaaa cagtacagaa atgatcatgg gaccacaaga
     8521 aaatatctat acgatccctt tattgcctga agatggcgcg gacacgtata aagaaagatt
     8581 cctctccgtt acagcaccat tgaaagatta cgtagtgctt tgcgatctgt tgggtgggac
     8641 acctgcaaat accgtttcca agctaatcat ggaaggtgaa acgatcgaac tatatgctgg
     8701 catgaatttg ccgatggtca tcgaattcat caattcgcaa atgattggag cagaaccaga
     8761 ttttattggt tcgggtcgtg agagtatcgt ttcagtgaac caagtgattg ctggcatggg
     8821 agacgatgaa gaagaataat caagcacaac ttcttgactg gtgatacaac tggatataaa
     8881 tacggatgta taaagaggcc gtgtataaaa aagccaacgt accgcgaaat atagttttct
     8941 atatttcaca gtacgttggc ttttatcgtc ataaaatcag aaatgatctg ggtcgatcga
     9001 agaagctagt tacaaataac tatttacgct tttgctgttt accagcattc cgaccattct
     9061 tcgcattgtt ttttggtgct tgtacttgtt tttttgtttg cttcgcaggt tcagcaggtg
     9121 tgacatcttt acgaggcgtc accacttgtt ttggcggatt ttgttttaat tcttccgcaa
     9181 cttgcgcttt taatcttggt ttcatgatga cattcgtaat gaatgtttgt agacaactaa
     9241 agatcccacc gactacccag taaagagtga caccagctgg ggcactgatc gacatgaaga
     9301 cgatcatagc gggtgatacg atcaacatcg tacgcatcgt tttcttttgt tcttcaggga
     9361 taccgatagt tgagatatag ccttgaagta aataagcaat accggcaatc gctactaaaa
     9421 ctaagctagg gttacctagg tcgatcccgt aaaaggttga ttcacggatg ccttctgtat
     9481 agcgagcagt gaaatatagc gcagaaaaga tcggcatttg gatcagaagc ggtaaacaac
     9541 cgataccacc agtcatactt aagccatttt cacgataaac ttgttgcatt tcagcttgtg
     9601 cagccatttg ttcttctcga gttgtcgcag ctttcaattt ttgttgcgta gcttcaactt
     9661 gtggtttcaa tgcttgcatc ttttcagatt ggatcattgt ttttttcgat tgactaatac
     9721 ctaatggcaa aatgatgata cgaacgatga ctgtcatcaa gataacagcc cagccgtagg
     9781 accaattaaa gttttccact aaatatgtaa tcgcttgtcc cattgggtga accaatatac
     9841 ggtaaatgat tcctgaagtg tcagggttac catttgaatc catgcggaca caaccggaca
     9901 agaaaagcat gatcgtgaca agcccagagc ccaataacca atttttccat ttattcataa
     9961 aatacttcct cttttataat ttttacacaa gcttaactat actaaaaaag cgctcataat
    10021 tcaatcaact tcatagaaat ttcatagaaa taaccgattg gaggatcaaa aaagacgctt
    10081 aatgttaaaa agaagaatcc agctaaaaat acttcagtat aaagcatact tagcacctcc
    10141 gttctctaaa ataagatgag caacggatat ttccttgaaa tcataatttt agcgcaaaaa
    10201 aaagaagtgg aatgtcaatt ttttccactt cttttttttt accattgagc cgatccataa
    10261 aaaagctgac tgccatggta taaggggcta actatccctg tgtcacttgt tctaactctt
    10321 tcattttctg gacaaccagt gctggtgtga tcgtttcttt catgaagtgg atcatttcat
    10381 caggtaaaca tgcacgttca gctacggctt gatagtcact ttctgttaaa tgtaaaccca
    10441 tatcagctaa actcgttggt aagttcaagt gatgataaaa cggcagtagg tgttcgattt
    10501 ctgctaattt attttcgatc atcagctgga caaggatacc gtatgcaact ttgtttccat
    10561 gaagttgctg atggctatca gggagtacgg tcatcgcatc gtgaatggaa tgagctccag
    10621 ctgttcggcc gtagtcatcg ccaaacccac caaccatacc agccaataaa atgtttgttt
    10681 ccacgacatc gataaaagct tgattgattt tttgctggtc catcgcttcc attgcctctg
    10741 cacttttctc taaaaggatt tcgcggcatc gtaacgctgc aaattccgct acttggactt
    10801 cgataggctt gatagttaac tgagagatga ttgcgtgaga ttcataccat tttgctaagg
    10861 tgtcaccaat accggcaatc atcatctcgc gaggggagtg caaaatgatc tttggatcga
    10921 tcaacactaa atcatttgct ttagggaaca tatcatagcg aagcatcact ccttcttcat
    10981 tatagaccac actaagtgga gtataagctg cgcacgtcgc agctaaggta ggtaagatga
    11041 tgattggtaa gtttgtctta taggcaatca attttcctag atctgctact ttcccgccgc
    11101 cgactgcaat aataccattg aaatccttct ttttgcataa agaaacaaaa ctgtcagcaa
    11161 gtggatcggt acattctcca ccataatatt caaaaatttt tgttattgat aaatctggaa
    11221 aaacatcttt tactgctttc caagatgttt ttccatggag aatcaaaacg ttgtcaatat
    11281 gccttttgta aaggtgttca gggagtgaat cccatgcgtt ttcttttatt tcatactctt
    11341 gaggggcacc gcgaacaatt aaattttttg tcatataaaa aacttcttcc attatttttt
    11401 taaattaatc tttttttatt ataatagtta tgctagtata ttagcaagtt atttatgaat
    11461 atttattctt ttatagcttt tctgattaca ttattattca aaggtggcca aatggatgaa
    11521 aaaaggaata aacatattat tagtggcgct gatgtttcta ctggtttttt taccagtaaa
    11581 agtacaaggt gcagaaaaag atgaaattta cgagcggata aaacaaagtg gagaattagt
    11641 cgttggccta tctgcagact atgcgcccta tgaattccat gcgaatgtgg atggtaagga
    11701 taaaatcgtt ggttttgatg tatccattgc cgaaaagatc gcaaaggata tgggtgtgaa
    11761 actaaaagta gaagaacttg gttttgatgc attattaggt gcgttgaaaa ctggcaagat
    11821 cgatttagtt atatctggta tggcggtgac tgaagaacgt ttacaagaag ttgacttttc
    11881 tgacccatat ttcgtggtac agcaaaaagt actcgttcgt aaagaggaca aaaataaatt
    11941 taagacgaca gccgactttt ctggcttagc cgttggggcg caaaagcaaa ccacacaaga
    12001 ggagttagtg aaaaatgaac tgactggtgc agagttgacc tccctgcaaa aagttcctga
    12061 tctgatcaat aatttgctga atgaaaaagt tgaaggtgtt gtgctggaac aaccagttgc
    12121 agaagcatat gcccaacaga ataagaaagt agctttgact ccagtaaaat ttgcgcaagg
    12181 cgaaaaaacc acagctattg cgatgtcaaa aaatacacca gaactgaaaa atcatgtcaa
    12241 tgcgtcaatc aaaacgatca atgacgacgg cttgctgaaa gattatcaaa aacaagcgaa
    12301 tcaattgatg ttccaagatg atcagtcctt tcttcaaaaa tacggtccgt attatttgat
    12361 tggaacaaaa tacactattt tattagcttt tgtgggtgta ttgtttggaa tggtctttgg
    12421 tagtttgctt gcactgatga aattgggtaa gaataaattt ttaaaggcat tagccattat
    12481 ttatatcgaa tacgtacggg gaacacctct cttagttcaa atctttatcg tttacttcgg
    12541 aacaggagta ctgggattgg atctatcgaa actggtagca ggttgtattg ctgtctcgtt
    12601 gaatagtgct gcttatgtag ccgaaattgt ccgtgccggg atcaatgcgg tcaacaaagg
    12661 acagatggaa gctgcacgtt ctttaggaat ggaccagaaa gaagccatgc gtttcatcat
    12721 cttcccacaa gcaatcaaaa atattcttcc agctctagga aatgagtttg tcacgatcat
    12781 caaagagtcc tcagttgttt cggtcattgg ggtatcggaa ttgatctttc aaacgggcgt
    12841 ggtacaaggt gctagcttca aaccattcct accatatgtg ttggtctctt tgatttattt
    12901 cgtgttgacc ttcagtattt cccgtatatt agctttagtt gaaaggagaa tgggtctgaa
    12961 tgcttaatgt aaaaaaactg tcaaaaagtt ttggcgagaa tgaagtctta aaatcaattg
    13021 atctttctgt gaaagaagga gaagtagtgg tgatcattgg cccttcagga agtgggaaaa
    13081 gtacaattct acgttgtatg aatctgctgg aagaaccaac ttctggtgaa atctttttta
    13141 agggacaaaa cattacagcc cctgacagcg atatcaatca aattcgtcaa aaaatgggaa
    13201 tggtatttca aagttttaat ttgtttcccc atatgaatgt actcgaaaat ttgaccatca
    13261 cacctgtaaa aatcaaaaaa gaagacccta aaaaagcaaa agaacatgct ctatcattat
    13321 tgaaccaagt tggtctaaag gaaaaagtgg acagttatcc taccagttta tctgggggac
    13381 agcaacaacg ggtagcaatt gctcgagcat tagcaatgaa tcctgatgtg atgcttttcg
    13441 atgaacctac atctgcactt gatcctgaaa tggtcggtga agtgttggca gtcatgcagg
    13501 atttagctaa aaaagggatg acgatggtcg tggtcactca tgaaatgggt tttgctaaag
    13561 aagttgccga tcgtgtgatt tttatggctg acggcatcat tcaagaagag ggaacaccga
    13621 aagagatttt tgaaaatccg caaaatagtc gaacacagga tttcttgaac aaagtgttgt
    13681 aacaaagatg gaggaagtaa gatggacaaa agtaagattg cacgccggtt tcagcaacca
    13741 gcagaaaatc tgttgatgaa tattgctatt ttggcaaagg aaaaagagga tattatcgat
    13801 ttatcgattg gtgatcctga cttgatcact gatccgacga ttattgaagc agcttataca
    13861 gatgtaaaaa atggtgctac taattatact gcgcctgatg gcagcaaaga attcttgtca
    13921 acggtcgtag atttttatca aaagaaatat ggattgagcg ttgaatgcaa ccaagttcga
    13981 gctacagtcg gagcaatgca cgggatgtat ctagttttac aggcattact agatccagag
    14041 gatgaggtca tcatccatga accttatttt tcaccatatc gggaacaagt cattttatct
    14101 ggaggcaagc ctgtttttgt tcctacctat gaagaaaagg gcttccagtt ggatgtggaa
    14161 gatttaaaag cagcgattac ggatcgtaca aaggtgatcg tcatcaattc gccgaataat
    14221 cctacgggag ctgttttttc tgaaagcacg ttgaaaaaaa tcgctgaagt cgcaatcgaa
    14281 aatgaactct atatcatttc cgatgagatt tatgaagact ttgcctttaa agagaagttc
    14341 gtaccgatgg cgcgatttgc gcccgataat acgatcactg tcagtggtgt atcgaagggg
    14401 tttgcgatga ctggctggcg cttagggtac atgatcgcac cagcatacat caatcaaatt
    14461 gctgggttga tcaatgaaaa catcacttat tccgcaccga ctgcttctca aagaggtgca
    14521 atctacgcct taaagcacta tgaacagctt gtaccgccag ttgttgaagt attcaaagaa
    14581 cgattagctt atattgagaa aagagtggca gaaatccctt acttatcttt atcttctgtt
    14641 tctggaagca tgtatgcttt tattaatatc agtaagagtg ggctcgattc agtaacgttt
    14701 gtggaagagg ttttagaaaa aagcggcgta ctttttgtcc ctggaaaagc gtttggggaa
    14761 acgatcgggg acaactatat tcgactagca gcgactcagc cattggaaat cttaggtgaa
    14821 gcctttgatc gtttagcaaa actaaatttt tagataaaac tgtatatttg aaaacagcta
    14881 tgggtatcca ttagaacaaa gagggaaaat gatcctttcg agagttggct tgttgactgg
    14941 gcagcgtcag gatttgacaa tgaaaaagtg gtagtggaaa atcaaagtcg attttccact
    15001 accataaaat attatagact ccattttatt gaaccaaaat ttttgaagtc ctcccgcttt
    15061 tacgtatgcc tatcactggt tactaaactc gctaataaga atgttttttt gtcgatctag
    15121 gatggagaga ggggcaaact ttttcgatct acatacagac agtccgattt ttctgacatt
    15181 tcctattcat ttctagaagg tcagcctatt acaacaaact tttttctgtc ctccatcatc
    15241 ggattttcct gaaggtctac atattggaca cgtccgctgg ggcttggtga tcgtttgact
    15301 tcttccacga attttttgat tgcctcatcg tcgcccgcag cttcgatcgt gacagagccg
    15361 tcatcttcat ttttcactgt tccggtgatc cctaattgat ccgctacgat ttttgtcata
    15421 tagcgaaaac caacgctttg tacacgtcct tgcacgttca tcttgatttt gttcatttta
    15481 ttcaccgttc tttcttttat ttttaaatta atgataccga cttttttgct actgaacaag
    15541 tgcaatgatc taatccatgg tataatcagt tgaggtgaaa aatgtgaaag agatccaatc
    15601 agcaaaaaat ccaagaatca aagaattaaa aaaattacat aagaaaaaat atcgtgaaga
    15661 aaaaggggaa tatctgcttg aagggtttca cctgatcgag gaggctgttg cctcaaatgc
    15721 gaaggtcaac gaacttttta ttacgcaacg cggtctgact gagtgggcta gttggcttga
    15781 agaaaatcag gcgctttctg ttacactagt atcagaagaa gtgatggcaa ctgtttcaga
    15841 tctaccaact ccgcaaggga tcatggcagt ggtggaagct gaaaaaagag agataccgaa
    15901 ttttgaaggt ggttggttgt tgctcgacaa cgtacaagat cccggcaatg tagggacaat
    15961 gatccgcaca gccgatgctt ttggtctatc ttgtgttgta ctaggtaagg gaacggcgga
    16021 tgtctacagt accaaagtat taagaagcat gcaggggagt aactatcatt taccgatcat
    16081 ccaagaggat ctaacaaaac tattgcctat ccttaaagag caaggattta taacctatgg
    16141 aacagaactg aatgaagaag cagtgtccct gaatgatatc cagacaacag aaaattatgc
    16201 gatcgtgatg gggaatgaag gtcaaggcgt acgtccagaa gtgctggcgc taacggataa
    16261 aaatgtgtat ataccgattc aaggaaatgc ggagtcttta aatgtaggag ttgcagcggg
    16321 catcatgctt tatcacttct ccgtatagtt aaggaactat taagctttct gcattttgcg
    16381 aaaacctcac tagaatatta cgaacactag tggtatacta agttcaattt caaagataaa
    16441 tggaattcaa aaaggtggca gatcgataat tatgacagaa aaatggcgtg aagacgaaga
    16501 atatatgtct tatataaatg atctattaga aactgatgca gtaaagaaat tagcaaatta
    16561 cacgcaacat gtccactcga cacgattaga gcattcaatc agtgtgtctt actatagcta
    16621 caaattagcg aaaaaatggg gcggtgatgc aagagcgact gctcgagcag gccttttaca
    16681 tgatttattt tactacgatt ggcgtacgac gaagttcgat gaaggtacac atgcgtatgt
    16741 tcacccaagg atcgctgtga aaaatgctga aaaaatcacg gagctttcag atctagagcg
    16801 tgatattatt ttgaaacata tgtggggagc aacgattgcc cctcctaaat ataaagaagg
    16861 ttatatcgtt acttttgtgg ataaatattg tgcagtcaaa gaagcagctc aaccaatgac
    16921 agcttcaatg aaacaaaaat ggcaacgata tttcggtaaa gaacaatcga tttaaggtag
    16981 ttaactaagt atttgacccg agtattttga aaaaaatcct cgggtttttt gtttagacat
    17041 tgactaattg gtctttttag tgtttacttt gttgagatga aaaagtacat aaccaacata
    17101 aaaataaagg gggtgatgat aagatgcgat gtgtatctta tttaaaagag tttgagggct
    17161 ttatctaaag gcactttgat agaaagatca cttttttctt caaagtagcc gattttgaaa
    17221 gaagaaagga ctcaaagtca tggaaaaaac aatcaaacca tatcaatatt attttatcaa
    17281 gcgagaagtc gagcagctgt taagcgccta tagttcagtg aatgatccaa agacagtcca
    17341 aacagtccaa gcattagctg cggaaaagat ccacgaggta cttggtcatc ctaatacagc
    17401 tgtaaatgaa tttatggaaa aggtactaga cgtcactcta aaaaaagaaa aagcagaacg
    17461 actattagag gaattgaaag tgatcgttct tccattcata caaccgacaa aaccgcagat
    17521 cgataaatta ttccgaaaag tgaaaaagtt aaaacaacca gattggaatc aactggattt
    17581 aagggaacat acttatattg gttggaatga tccaggtagc caaaaaaaat ttatgatcta
    17641 ttatgaagaa gaaaaactaa aagggatcag tggaacatta tctcctacaa tcaacaaagg
    17701 aatttgttcg atttgtcaaa gaacttcaaa tgtttcactc tttctttcta ccacaaaaac
    17761 tggttcagat ggaacataca cgaaaaaagg aaattatatt tgtcatgata gtgaccaatg
    17821 taatcagcag ttggtacaat tagctccttt ttacgaattc acagaacgag tgaaacgtga
    17881 gcggtgattt cattgtattt ttctgaaaaa tatgctatct tatttggtga agtgaatagg
    17941 tgatggtgtt cgcctttaaa cgttgatcgt aagataacta atgacgccta ctaaagaaac
    18001 ggggctaccc ctttttttag taggcgtttt tttgaggagg aaatgagatg gaaaatcgat
    18061 tacctgatcc attgaaggtg atcatctata tgatcaaatg gttattgatc acagcaattt
    18121 taggtatttt tatgggaagt ttatccgcat tctttctaac tagtttgact gatgtgacag
    18181 ctattcggct ggagcatcct tggttacttt ttcttttgcc gataagtggg ggactttttg
    18241 cttatctata tacgacgtat gggggcaatt cttcccgggg aaacaattta gtgatcgatc
    18301 aagggaatgg aggaaatgaa aagattccat tacggctgat cccactgacc ttatttggca
    18361 ccattaccac gcatcttttt ggtggttctg ttggtcggga gggtacagcg gtccaaatgg
    18421 gcggggcact tgctgagaat tttgctcgtt ggctaaaatt gaacaaaatc gagcgtgaga
    18481 ttcttgtcat cagtgggatc agcgcaggtt ttagctcagt atttgggaca cccttggctg
    18541 ggacattgtt tggattagaa gtattggcaa tcggaaaagt gagaaccgaa gcactgtttc
    18601 caagcttttt tgcagcactt tttgcgaact ttgtaacgga aagtttcaat gtcaagcata
    18661 cccattatca aatgggggcg attcctgcat gggacccttc acttttcttc aaattgttgc
    18721 ttgcgggcat tgcatttggt atcattggct ggctctttag ccgttcgatc gtctggttaa
    18781 aagcttggta tgcaaagcgg atcccccatc cagttttacg caactttatt ggaggagcag
    18841 tcgtggtact cgtagtagtg attttacaga cgcaaagata tctcggatta agcttacctt
    18901 tattacaaga tgcatttaat ggaagtgctc atccttatga ttttcttggg aaacttggct
    18961 ttaccgttct atcacttggc gcgggatacc aaggaggcga agtgacccct ttgtttgaga
    19021 ttggggcaac tttaggtgcg acattagcac ccattcttca tgtatcagta ccttttttag
    19081 caggattagg gtttattggg gtgttctctg gagcaacgaa tacaccgatt gcttgctttg
    19141 ttatggggat cgaacttttt ggaggtcaag cagccagcta cttctttatg atctgtttga
    19201 ttagctttat gtgttcagga aatagtggaa tctattctgc gcaaaaagtt gaagtgaaaa
    19261 aaggaatctt gttcctaccg atccttggaa gaatctcacg acaaaaagag aaataaaaag
    19321 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    19381 taaatttttg tgaatttcgc ttatttccga aggagttgct tctgctccca ccgtttattc
    19441 ggagttagag tctgtggcaa aactgatttt tagttttgtc ccacgctctt ttttattgag
    19501 caaatttgag aataagagca gaaaatactg caaagccaat cattaagcca cagatgataa
    19561 gatggcgttt tttgtgtaag acaaggatgg caataaattc tcttaagata gcattcggta
    19621 agtagtatag tgcagttttt gcaccgattc cgtttgcatt gagtttcgct tgtcgcgcat
    19681 atagtccagc tctaaagata tggtaattat ttgaagtaaa aatcgcgcga taaggcccat
    19741 tcatttgctg atccattatt tcttttgaaa acagcatatt ttctagcgtc gttgtggaat
    19801 tcgtttctac taaaatatca cgctcgggaa tccctttttc taaagcatat tgtttcattg
    19861 caatggcttc agctactttt tcatcggttc cttttcctcc tgacatgagc aagactggcg
    19921 gattcaaagt tgctttactt tgtgcccaat aaaatgcgat cgccttattg atccttttag
    19981 caagtagggg agatacgcgt tcaccatcaa tcaagccagc gccgagaaca acgatataat
    20041 cttggttata ccttggacgg ttgaactgat acaaaattga aacagtcata aaattaaaga
    20101 aaacgatgag tagataagca aaaataaacg gaatgacagc aaatagagca tttgcccatt
    20161 gtgggagcca atggactaat gtatagtcca atatcaaaaa gatagtcaaa gcgactccta
    20221 acaagagagt caaaaggttg gccaaagaac gggattcttt tttcatgact acaacaccat
    20281 tccaaaataa aaaaatgatc aaaatgtaga taccgattgc caaaagaatt aatattggta
    20341 tcccgatcaa agccaataac ccaccgataa agatattttg cgtctctatt agtagccacc
    20401 ctagataacc accaaagaca atcaaaaacg tattaaaaag tacgccattg attaacctac
    20461 gtttttcctt aaaatagaaa aatgcaaaaa tactgaaaaa tagcaatgga atcaaaaaat
    20521 aaaatgtatg ataaaaaact tctaaaacga cgaagtaagt gaccatcatc ataaaagtcc
    20581 acgtcccaca ataaaagaat agagcgggat cgctttggcg cttgcgccaa tagaggatgc
    20641 ctgacattcc taaaatatag ataactaaaa atatcaggtc tctagtaaga ttcattgaat
    20701 gaactccttt cgatttgtaa attttttctt aaaaaagata gcatcattta gtagtaaata
    20761 tatcgaatat agaaaagtta gttactaaat agtcaatcag ttttttcacg acgtttctat
    20821 tataacgaat tatgttgaaa aaagctttta tcgaaaacat aaatcttcaa attaacagat
    20881 tttttagacc tcgatcatga tgaagacttt tgtagataca aataactgta acattgaaaa
    20941 tgaatgcgaa aacgatattt ttttttattg gaaaatatcg gtcaagcaat aaaccagttg
    21001 cataataaaa tgttaacggc aacaaaatct gtggaaggaa tgaaccggat gatttgatgg
    21061 aaaaaacaag atagacaaga agtatacata gtaaaacgtg gaacaagtag gccagaatga
    21121 aaaatcagtt tttatccact cgcttttcta tacattcact gtcacaaata taatcgaaag
    21181 taattctatt ttgagataaa gtattttata ggagatagta tatgaacaaa aaaagattta
    21241 aacgtattga aagaaaattt gcattaactt atgaccaagc taagtatttg aaaacactat
    21301 ttccaaaaca ggtagttcgg gataaatata gtgatcattt tatttcttcc ttgtattttg
    21361 atacaaacga tcatcacttt ttaaaagaaa tcactacaga aaagtcctat cgagaaaaat
    21421 ttagaatcag aaaatatgga gatcctcagg acatatcgcc tatctactta gagataaaaa
    21481 gaaagtttca aggaatgaca gataaacgta gaataaaatt agcgcatcat gagctcgatg
    21541 atatgttgga aaatatcgaa ttggcagaaa ccacattcag taatttagaa gaagagtata
    21601 tttttaatga aatacagtgg ttgttcagaa ggttggcatt ggagcccaaa gtatatatct
    21661 attatttaag agaagcattt gtaaatagag attcagaaga tttgcgaatc acttttgatc
    21721 aaaatatcca ttaccgtcta atgaacttaa cgacctggga acactgtaca gaagacaatc
    21781 tggtagcgcc agaaatcgat gtgctgatgg aagtaaaaac gacaggagaa atacctgaat
    21841 ggttacaggt aggattggat aggatgggta aaaaagagat aaaattttct aaatataaac
    21901 aagtctataa aagatatatc caagaggacc cgctctatgt tcaatagtgt cttaaaaaat
    21961 gagccttctc aactcacact agatgatttc tttttatgta tggtggtgtc gattttgcta
    22021 ggcattctga ttgcttacac ccatatgtat caaaacaact gtacaaaaaa cttcatgttt
    22081 gccttgattg ggttacctac tttagtacat accgtcattt tgttgatcaa cggtaactta
    22141 ggcacgggga tcgctgttct aggcgcattt aacttagtgc gatttcggtc tgctcaaggg
    22201 aattcaagag atattttcag tattttttgg gcaacgtcga ttggtttagc cacagggacg
    22261 ggatatattg tctatgcact attcgtgtct agtatagttg ctgctatcca agtcatcatc
    22321 ttcacgcttc caatcaaaaa taaacataca gtttttaata ctgaaaaaac gatcaagcta
    22381 aaagtttcta aatccttgtt tctttcgagt attattgaag aaaaaattgc agaatataca
    22441 gaaaactgca atttaaaaaa agtaaaaatc aataaagaaa cttgttcctt acactatata
    22501 gtaaaagtaa aaacgagtat gcttgaaaaa gaattggttc gaacgatcat ggatcatcca
    22561 gaagtcattt cgatcactgt aggtatggat aaatcgaata aattattgtg ataaccaaga
    22621 aagtacttaa caatgaagta agaccccagt tccaaagttt agcggggaca cttcaataaa
    22681 gtactttctt ttttgtcttc agcaactact ggtaaaactg agagcaagct ttcgtgagag
    22741 tacagcgata aagatcaacc tcccttttta tctcgagcaa tttccttgca aggctgtttc
    22801 gttgatctta aacgagttag caacagtcaa agatgcgatg gccataaaaa aattgcatta
    22861 agttggctgg tatctgatag ctgggaatct aaggaagact atccattctg tttcttaaat
    22921 tccgctagtt gatagatatc agccaattgt tccagatgat caacatcaaa gataaccgaa
    22981 agatgtttcc ttttattccc atttgcttga agagttttta tcttctcttt ggttccttca
    23041 ctcaatgaga gtgtgactaa tttctccctc gttttttctg ggaaatgaaa ggatactttg
    23101 aaaaatccgt cccaaataga aagccagaag atcggtttta ctttctgaga gtgggacaaa
    23161 agtaagaaac acttttgtct cattctttaa aaacgaataa acggtgggag cagaagcaac
    23221 tccttcggaa ataagccaaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
    23281 ttatttctcg gagttaaaca cttctgtccc aacctcttgt attcaccttt tgatagccaa
    23341 gcttttccat cattataaaa ccgcaaattt caaaaaaaaa cgaacaatac aaaaagcgta
    23401 ttgctcgttt ttctcttcag tttctcacac ctaaacgact tttgtctcga cctctttttg
    23461 gtgactattc atgatgttaa aataaattac tgaaaaaatc tgtgacatgg cgccaaccaa
    23521 tgacaaaaat attagctttt tcaacagtag aagtggtaat gatgtctgtc gaaggctctt
    23581 ggtttgcttc caaatagcct aatttgtcat cagctaattg gatcgttgct gagccaactg
    23641 tagtgccttt gttgatagga gcggtcactt ggtcatccgt tactttcttt ttatcaattg
    23701 ttggagtaat cgttaatttt ccagtatcca ttccattttc gacccagact tttacagggc
    23761 tttttagcgc aacattgacg gaggtttctt ttccatcttt cactttgatg tcttttaggc
    23821 ttgggatgct tgcatttgct tttccaagtt ctttttctga ccagttgtca taacaataat
    23881 ccatcaatcg tgatgtttca ttgaatcggg cacttggatt ttcttcatgg ttatctgcat
    23941 ttaaaacgac tgtgatgatt cgtcggccat ctttgttgat cgttccgaca aagcatgcac
    24001 ccgcaaaatc agttgtgcca gtttttaatc catcgacacc ttcgtgatag tttacaaaac
    24061 caggtagcat ccagttccaa ttgaccattt caaccggtga ctgtgtgttt tcgccgaatg
    24121 tttgatttgt ggtactcgtg atttttaaga cttcaggata atctaagatg aggtgacgag
    24181 caacaatagc gacatctttc gcagacagtt gattttcatc tgtttcacca gtattctcag
    24241 gtcgattctc acctaggtaa gaattgttta aacctgatgc attgacgatc gttgcatctt
    24301 tgatacccca atcttttagt tgtttcttca ttagctcaag aaatttactt tcactgcctg
    24361 caactttttc agctaaagca accatggaag cattggctga ttggatgaaa gctgagtcaa
    24421 caagttcttt cactgtataa tcattttctt tatgcaaagg aacattcgat agatccggca
    24481 cgacacttaa tttttctgca taatccgaaa tggctacctt atcttcccaa gataatttac
    24541 cctctttgat ttgttcaagt acgatatata gaccaatgat ctttgtgatg gaagcaattc
    24601 ccatcgtttt ttcgccatcc tgatcataaa ggatttttcc actttctgca tctacggcaa
    24661 aggcagcttt ggcatttatt ttaaatgcgt cgtctgcttg aacagttgcg ctaggcaaga
    24721 aagaagtgat acataagata agacttgcta atagtacaat aaaacgggta ttttttttga
    24781 acatctttgt tctcctcatt tttaaattga ttcttacaac ttagaatatt atcagagaga
    24841 acaaaaggtt tcaatggttc tgaaaaaaac cttataaaga acaagtctta ttaatgaaga
    24901 atcatagaaa gttatctttt gggttcattg acacgaactt tgactggctg aacttcgggt
    24961 tcggttaggc gtgatgctgc aaaaagggcg cctgctgaga gtgcgaccaa actaaaaatc
    25021 atgaataatt gcataagatc acctcgttct tttttgtttt tttatagtat agcaagagag
    25081 tttgaagcta ctgttaagga aatgagcata taattgatga aagtatgaaa aaaatatgaa
    25141 gtaggacaac acaactaaaa atcgatcagg tatttctctg gtagggattc gttttattgt
    25201 attgataaaa tgggatgtgg tagactttat ttacagatac aaagaaatga tttttttagg
    25261 aggggtacgt ttatggcaaa aaaagaaaaa gaattattat cacaattgac ttcagaaggt
    25321 accaaaggac cgtttgtcac gattatgtta aacacccatg ttgcgcatca agacgtagaa
    25381 aaagatcaaa tcaaattcaa gaattttgca aaggaagcga aaaagcgctt tgaaaagaaa
    25441 tactctgagc atgattggtc tccattccaa gaaaaaatta ctagtttgtt ggacgatccg
    25501 tctttctggc gtagtgccac aacgagtgta gctgtcatag tatcaccaga acaagtgttt
    25561 atccaccgtt tgggtattcc tgtagatgac cagtattatg tgagtgatct accttattta
    25621 ttagcgatca tcaaaaatgg acatttcaac tattcatact atttattagg attgaatcgt
    25681 gattcgatga aattgtacga agtaacgaat aaacaagtca aagaagtggc tttgcctgaa
    25741 gatgcaccaa gagatgtcgt cactgcttta ggatctgaac taacaggagg cgggttgaac
    25801 tacgcatcaa atggtagtga aggtacgacg ttccatggag tcaatcctaa agatgaagaa
    25861 gtcgaaattg attgggtcaa ttattatcaa gcagtcgatg actatttgaa agatacattt
    25921 gaaaatccag agaaatggcc tatcttctta tttgcattac cagaaaatca gacgatgttc
    25981 aaaaaagtgg cgaagaaccc ttattatcgt tccgatgttg cgatctcagc ttcacctgca
    26041 caactttcat taaaagaaat tgaagcaggc gcaaaaaaac tatcgcaaga attagctgtc
    26101 aaagaagcaa gtgactacaa taagttgctt gataaaaagt tcattgatca actcgttgat
    26161 attataccgg cagcaaaaga agggaaaata tcccatttat tcattgctac atctaattta
    26221 gcagatggtt ttggcgaaga tccagaaaca gaatatgacc gccgtcaggt gctgaatgaa
    26281 ctagcagata atgtgatcac gaacggtggt caggtcttta tccttgaaca aaaagatgca
    26341 ccagacgaaa agagtttagt tgcgatctta cgttattgag tgaaaactac caaagtagat
    26401 gaatggtcag gcgttgatag aagtgtatag ctccgagaga aggagtttcc tctgtttcag
    26461 ccgttggatt catcttgagc gagagacaaa agctagcttt tgtctctcgc tcattatttt
    26521 tgtgctttag tcccattggc tttgcgaggg gaagatactt ttctcaaaaa aacgaacctt
    26581 gcagttgaga gggggataga tgcgcatact aattttta
//
LOCUS       DAYYAJ010000027        26420 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000027 DAYYAJ010000000
VERSION     DAYYAJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.027"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..1509
                     /locus_tag="ACHATZ_000969"
     CDS             58..1509
                     /locus_tag="ACHATZ_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8403517.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1858..2490
                     /locus_tag="ACHATZ_000970"
     CDS             1858..2490
                     /locus_tag="ACHATZ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8403518.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3078..4517
                     /locus_tag="ACHATZ_000971"
     CDS             3078..4517
                     /locus_tag="ACHATZ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8403519.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4812..5030
                     /locus_tag="ACHATZ_000972"
                     /pseudo
     CDS             4812..5030
                     /locus_tag="ACHATZ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5279..5494
                     /locus_tag="ACHATZ_000973"
     CDS             5279..5494
                     /locus_tag="ACHATZ_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403520.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5711..5967)
                     /locus_tag="ACHATZ_000974"
                     /pseudo
     CDS             complement(5711..5967)
                     /locus_tag="ACHATZ_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6154..7065)
                     /locus_tag="ACHATZ_000975"
     CDS             complement(6154..7065)
                     /locus_tag="ACHATZ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8403521.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7084..8088)
                     /locus_tag="ACHATZ_000976"
     CDS             complement(7084..8088)
                     /locus_tag="ACHATZ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8403522.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8085..10208)
                     /locus_tag="ACHATZ_000977"
     CDS             complement(8085..10208)
                     /locus_tag="ACHATZ_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8403523.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10213..12660)
                     /locus_tag="ACHATZ_000978"
     CDS             complement(10213..12660)
                     /locus_tag="ACHATZ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8403524.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12647..13036)
                     /locus_tag="ACHATZ_000979"
     CDS             complement(12647..13036)
                     /locus_tag="ACHATZ_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8403525.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13096..13599)
                     /locus_tag="ACHATZ_000980"
     CDS             complement(13096..13599)
                     /locus_tag="ACHATZ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8403526.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13612..13836)
                     /locus_tag="ACHATZ_000981"
     CDS             complement(13612..13836)
                     /locus_tag="ACHATZ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403527.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13940..15850)
                     /gene="ltrA"
                     /locus_tag="ACHATZ_000982"
     CDS             complement(13940..15850)
                     /gene="ltrA"
                     /locus_tag="ACHATZ_000982"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8403528.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16596..16733)
                     /locus_tag="ACHATZ_000983"
     CDS             complement(16596..16733)
                     /locus_tag="ACHATZ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8403529.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16730..17914)
                     /gene="mobT"
                     /locus_tag="ACHATZ_000984"
     CDS             complement(16730..17914)
                     /gene="mobT"
                     /locus_tag="ACHATZ_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8403530.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18173..18463)
                     /locus_tag="ACHATZ_000985"
     CDS             complement(18173..18463)
                     /locus_tag="ACHATZ_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403531.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18453..19595)
                     /gene="dcm"
                     /locus_tag="ACHATZ_000986"
                     /pseudo
     CDS             complement(18453..19595)
                     /gene="dcm"
                     /locus_tag="ACHATZ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19671..19835)
                     /locus_tag="ACHATZ_000987"
     CDS             complement(19671..19835)
                     /locus_tag="ACHATZ_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403532.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19828..20502)
                     /locus_tag="ACHATZ_000988"
     CDS             complement(19828..20502)
                     /locus_tag="ACHATZ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8403533.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20580..21920)
                     /locus_tag="ACHATZ_000989"
     CDS             complement(20580..21920)
                     /locus_tag="ACHATZ_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8403534.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22003..22338)
                     /locus_tag="ACHATZ_000990"
     CDS             complement(22003..22338)
                     /locus_tag="ACHATZ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8403535.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22348..22722)
                     /locus_tag="ACHATZ_000991"
     CDS             complement(22348..22722)
                     /locus_tag="ACHATZ_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8403536.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22735..23049)
                     /locus_tag="ACHATZ_000992"
     CDS             complement(22735..23049)
                     /locus_tag="ACHATZ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8403537.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23162..26389)
                     /gene="fss3"
                     /locus_tag="ACHATZ_000993"
     CDS             complement(23162..26389)
                     /gene="fss3"
                     /locus_tag="ACHATZ_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8403538.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7643 a         5519 c         4614 g         8644 t
ORIGIN      
        1 tttatcataa tttttaatag aaaacactat taaagagaaa aaagagaggt aaatcaaatg
       61 aaaaaatctt taaaacttgc atcagcagtt atattaatgt cccaactcct tatagcaaca
      121 cctttccatg tgttggcaag cgaaaattca acatacaaaa ttgcaaaaga aagaaccgag
      181 acgactgaaa tgagtaattc cgatgtaaca gcagagagta gtgaactttt gacaacttca
      241 tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt
      301 agtacgactt caaatgccga gtcttcaaca acttcatcaa gtgaaagtag tacgacttca
      361 aatgccgagt cttcaacaac ttcgtcaagt gaaagtagta cgatttcaag taccgaatct
      421 tcaacaactt caagtagtac aagtgaaata acttcttcga aagtgacaaa accaaaccat
      481 atagaatctt caaaaaagga agcgccaaaa acggaaggac aaacaaaaac tttttcgaca
      541 ccacagcaaa tagaatcaac agaatctttc caatcacaac caatactatc tggtaatgaa
      601 gaaattcatt ttgacaaaaa tgaatctgta gaaaatttta ttatgaaaat tggtgagtct
      661 gcgagaaaaa ttggtcagaa atatgattta tatgcttctg ttatgattgc acaagcgatc
      721 ttagaatctg ccagtggtca aagccagtta gctcaggcac cgaattacaa tttatttggg
      781 ataaaaggaa cttataatgg aaactttgtc attatggtaa caaacgaaga tttgggtaat
      841 ggaacgcttt atacgaccca atcaaaattt agagtgtatg aaaactatga ggaaagcttt
      901 gaagattatg ccaaattatt aactaaggga attagtggaa ataaagattt ttatgcagga
      961 gcattaaaag ctaatagtaa aacatatcga gaagcaacta aattccttac aggacggtat
     1021 gcaacagata cacaatacta tttgaagcta aatgaattaa ttaaaaccta cgatttaacc
     1081 aactatgata aagaagttaa aggtccacaa atgagtaaga ctggatacca agtgcctatt
     1141 cataattatt caataaccag tttgtttgga aatcgtggaa atgaatttca ccgaggactt
     1201 gatatggcag ctacacaggg tgagccgatt catgcagtta aagctggtaa ggttgttaaa
     1261 gcagagtttc actactcttg gggaaattac gtggtgatac aacacgaaga tggatcaacc
     1321 gcgttatatg ctcaccagca acaataccta gtaaaaactg gagataatgt tttgcaagga
     1381 caaataattg gctatgtagg aagtactggg aatagtactg gcccacacct tcatttggaa
     1441 atttgcaaag atagtagtct ttctcagagc atgttagttg atccgaaaac cttattattc
     1501 ggagactaaa aaaggtaatc aatctgacaa agtaacaaga aaatggttgg attcttagac
     1561 ctttgatcag gatattgaga ctggaattga tatctcgatt acgatgggtt tgacaaataa
     1621 aacaagtcca ttatcgaata tcgagtgtat tattgccgtc tttatgctca tattttgagc
     1681 aggtttcact agatgaaaac aatttatcta tcttgaggat ttcgtgtacg tatcgttcag
     1741 tcttgtattg taaattagtc ataaaatcag actaagaaaa attggaaata tttttttctg
     1801 atttattaaa agttaattga aattatatgg tacttctttc ttgggagggt aggtgcagtg
     1861 aaggaaagat atggtcacct tactgttgtt gaggagatta ctcataatgg atataaaaaa
     1921 tatcgttgcc tatgtgactg tgggaatgaa acgattgtat atcgatcaaa cttactatca
     1981 ggtagaacca aaagttgtgg atgtggaaat tataaaaata ggcataaata taaaaatttg
     2041 acaaataaaa cttttggtaa gttagttgca atcaagccaa ctaaactgcg aagtgaagga
     2101 actgttgttt gggaatgtca atgtctgtgt gggagaaaaa agttagtatc ggcaaagcga
     2161 cttctaaaag gtgatgtaaa aagctgtgga tgtttgaaat caaaaaagca tcacattgaa
     2221 aataaaaaat tcggattact tactgcaaag tatcctttgg acaataactg ttcatggaaa
     2281 acaaaatggg tttgtcattg tgagtgtggc agtatgtgtg agataagtta tagtaactta
     2341 ttttttggac atactcaaag ttgtggatgt ttaaaaaggc aagaaacaag gacatgtgtt
     2401 gaagggacag taatagagtg tctaacgtca aaattaccga aaaataatac aagtggtgtt
     2461 aagggagttt atcgttctaa agagaaatag attgcgtata taactttaaa aaaaaagagg
     2521 tactatttag gatcgtatga ttctattaag agtgcagctc aagctagaaa agatgctgaa
     2581 aataaattgt ttcaaccttt gattgacaaa tatcgaggta atcattaatt attgtggata
     2641 ataattaaca gtgatatata aacatctata atatattatt catactattg atatttagta
     2701 taatcctatg gaattgaata tcatagcaat gtatttgaaa agcaaattgg aaaacaaata
     2761 ggtgcaacac cataaatatg aaaaaattgg gaaaaagtag aatggaaatt tgcttacgtc
     2821 tcacgagata gctcacacta ttaatctgaa gaacaatgaa gtcgactttt agaaattgct
     2881 agaagaaagt aaagcgaatt atcactagtt actatagagt aagttcaaat aaagcatctc
     2941 caacttacat ggttacatga ggatatcaaa tcgaaattac tcaagcttcc tatggctaga
     3001 gtcgcgagaa gttgttgggc agtagacata ggttatattg ttcttagcta cacgatcatt
     3061 agaaaggaaa tcctattatg tatagttatc aagaagatgc aaaagtccaa aaaaatcgct
     3121 ggtggatttt gattgctata tctatgttta ccttcatgtc cacattggat ggcagtattg
     3181 tcaatattgc actacctacc atctcagatg atatgggcat accaatgaat caagcggaat
     3241 gggtagtgtc cgtttatctg atgattgtct gttcttgttt gttgctgttt ggtaaaattg
     3301 gcgatagccg tgggaaaatc aaagtatttc ggattggtac gctgatcttt gtcttgggat
     3361 cgctgttgtg tgggttcaat cattcgctag gtattttatt gttcgcacgg gtggtacaag
     3421 ccattggtgc tagtatgact atggctacaa atgccggaat catcacggaa gtctttccca
     3481 tacatgaacg tggtcgggca ttgggagcaa taggtgcgtt tgtttcatta ggctcgattg
     3541 ctggaccagg tcttggcggc ttgattttgt cccaattttc ttgggcgtac attttttgga
     3601 tcaatgtacc ggtgggaatc gtaacgatgt tgattgctga aaaattctta ccaaaagata
     3661 tcaccatgag cggccataca atcgatggag ggggctttgc tgccattgcg gctacgatta
     3721 tcagcttttt tggagcaatc tttttggggc aagaaagtgg ctttttccat ccagtccccc
     3781 ttgtactgtt cgtaatttca gtgattgcct ttgtcagctt tttaaaagtt gaaaagaaag
     3841 cagcctctcc attgatttct ttcggtattt ttaagaacaa gatttttacc atgagcttga
     3901 ttacagcctt gttgattttt tcttccaact tctttgtcaa tgtagtgatt cctttttatc
     3961 tgcaaaatgc tcgcgggttg agtcctagct atgctgggct gttgatgatg gtctttcctt
     4021 tgatgatggt cgtcggggcg cctttaagcg gctacctgac cgaccgggtt ggcccggaga
     4081 ttttggtatt agtaggcttg ggcttattgt cggtaaccca ggtcatgtat atgtttttgt
     4141 cagtggactc tcctttgtgg ctatacattg tcgctactgc catcatgggt gccggcaatg
     4201 ccttgttcca gtcccccaat aatacgatgg ttatgagctc ggtcactatc gaaaacttgg
     4261 gactggcagg ctcgatgaat tcctttgccc gaaatttggg gatggtcagc gggatttcgc
     4321 tttctacgac ggtcttatat caagcgatgt ctgctaagat gggccagcaa gtaactaact
     4381 atatcgcaaa tcggccggat gtctttttat atggaatgaa gattaccttt ttaggcagct
     4441 ttgttttgtg ttttgttgcc tttttcctga ctatctggcg tatcaaaaaa caaggaggta
     4501 ttcagaaaaa tgtctgaaaa aaccactgtt gaagaatttt tggaggcctg cgtatgggtg
     4561 agtgggattt ccatggatga tggaagatga agtgaacttc taaaagccga aaaactcaag
     4621 tattatcttc aaaatactag tgctcaatga gatgcttgat ttgatcacaa gaagactgcg
     4681 aaagccgctg gcaaccgcaa gctaggtatc ccaccgatgg actacgtttt agaggctact
     4741 ggttatgaat acggagccaa cgctctcatc tgcataagaa tacatcaccc agattatgta
     4801 aagattttcg atacttctgt caaaaaatca ttaagataga taaatgattt ccgtctagtc
     4861 aaattggttc agaatgtaga cacacagatg gtaagaagtc actcacaatt cgagaatgga
     4921 cttgtcctat ttgccgtata catcataatc gaaacgtcaa cgatagtatc aatattttga
     4981 ccaagggcta tgaatacaag cattgcctta gaaaacatca agaaccttag gaacaacggg
     5041 gatagcttgg ttaataagag ataccgctgt ttaggcttgc aagtatactc ttttatcaag
     5101 aattccgtgg ctttagtcat gagaggctta atactatgaa aattttcttt agcatttttg
     5161 atttaggttc cttttctaat tataataaga tgaccactac agtaacctac tgaaatatat
     5221 aaacagccac tgtagtacca ttttcaaagt agtctatgag aggagaatct tcattcttat
     5281 gaacgctgga attgcggata tgaatttaat aaaaaaaacc cttaatgatt ttacttccaa
     5341 ttcaatatca aaaggtactg gtatcaattt aagcacaata aaaaagctga aaagtggtga
     5401 aagatcagtg gaaaagctga atcttttaga tgctataaaa ataacggaat ttgctatgaa
     5461 aaatggaaaa gctgagattg aaatatggag ataattatcg tctagtgaat tacttgccac
     5521 ttagctgaat ttaacgatcc ttggtgcttg aagggggaga ttcttgggaa tagagcatac
     5581 ttgcaagcct atgtctttac taacaacgat atctcttatt aaccaagcta tccccgtagt
     5641 tctaaggctt ttacttttat ctaagcaata atcgtattca aaggaaattt ttgaccctta
     5701 gaaagtctca ttttatcaga cgtgagctta ggcattgctg aattttgtta attcggtttt
     5761 aggttgtgct ttgagcataa tatgaaagta ttctttatca cgatttcatt catctaaggt
     5821 aatgtgatgt gattctgaaa ttctttcaaa ggcagttttt gcataagctg acattacatc
     5881 atcaatgaca tgtttacaat gtttcactac taaaataagg gggtaaccaa gaagaaaaac
     5941 tgaatgatta tttgtatcta attccatggt atatcaacca tttcctatat agactgatta
     6001 tgacatggat taaaataaaa aggcactatg tcttaattgt cggttttcga gttaccgaat
     6061 ggcattcatc acccacctat agaggatggg cgacttctgc ctgaaataag ttacactact
     6121 tataacttct aataaaatta gattgagtca ttattattta acatcaacga ttttccagtt
     6181 tccatttttt tgtaatacta atttgtactg aaagtagcta gtggtttttg atacattatc
     6241 aaggtatttt atctttgtac tcactagaaa attttttccg ttttgtagat aaactggatt
     6301 ctctaactcc gaaaacttta agtcaatatt gattggtcgc atcgcaccat tttcaacata
     6361 atactccaat tctttttcag aagctgatgg ataaagttta aaaaatgttt ctaaaaaatc
     6421 ggtgatttct tccgttgttt tggcatcaat ttctgaatcg ttttctactt gttttgcttc
     6481 atattttgcc ttgtctggct ttccaatcat tgttggcaaa ctagttacta aatgattcct
     6541 gttttcatct tcaaagattg tcactcgata tgcagaagaa attgattgcg tcttttttcc
     6601 ctctgtaata tgttgctcca ctaaaaatga aacgacaaat tcttccgagt gtttttcaac
     6661 atctagaatt tttacagatt gaacttcgga actcgtggga atatctgctc gaaccatatc
     6721 ttggattaaa gcgagaccgt tttctgtaag gtactgttcg agattactca ttctcttttc
     6781 aattgcttct ttgttatttt tccatgaaaa atattctttc gcaaaatcct ctgtaaaact
     6841 ttcaacgcca gaagtattca ctaccttctc ttttataacc actttttcat gagttgtatg
     6901 ttgatcaatg gcggtgaaat ttttataaac gccaaaagca agactacaag acaataaaag
     6961 ccaaagggca agcaccattt ttcgatgctt acccacggat acaactcgtt cttttttctt
     7021 ttttgggctc ttttcctttt gattccgttc aatttttatc ttcattcttt ttcctctttt
     7081 ctcttatttg tatcggccag ctccgataag atgctgccgc caatagtttt ccgttagatt
     7141 cgtccacccc actggatctc ctgcatgaaa catgcgattc tcccctgcgt aaatgcccac
     7201 atgggtcaca taagtaccgg catcataggt tgaatgaaag aaaactaaat cacctggttt
     7261 ggcatctttt agatcaatat gagtcatggc atcatattga tcctgagcca cccgaggaag
     7321 tttcaggtca actttggcat aacaccattg ggtcaagcca ctacaatcaa accccgtttc
     7381 tggacttgag ccaccaaaca cataggccgt tccttcatac ttaaatgcct catcaaaaat
     7441 ttcttggact attttgtcat caaactgagt tgtagttagg tattgcttca ctaataaaca
     7501 gtaaaacata ttgccataat tataccgcca cccaccattt tccttaatcg cgattgggtt
     7561 ggggtaatcg actttttgac cacctgattt ttcctttgaa aaagattcag ctaaagcaaa
     7621 actatgcttc ttcccgtttt tcccgacatg atctagatac ccgccaccat agttataggc
     7681 ctgaataata ctatcttgat cacaccctag ttctttggaa cgcttaacca aagacgcaaa
     7741 atagagacag ccttgtttga tggatgcctc ggtacttaat gaattaggtg gtaatccggc
     7801 tgactcagaa ctttgcataa cgtcttccat tgtgccacca gattccactt gaataatcgc
     7861 caaaagaatt ggaatatact ctgttatatc cgattctttg gcatactttt ccaccattgc
     7921 acggtgtttc aatacttctt ctgagacact agttccgcca ggagaagtgc cagaactacc
     7981 atttccctca ctgtcttcac caaacaacaa acccacacac atcaaaatac ccataaaaga
     8041 gatggtcaga aaagttaatg aaataacacc cactttcttc aagttcattt tttctcacct
     8101 gaccttgatt ttggacgatg gttcattctt ttttgtactt tttgattggt ctgctttggc
     8161 agagtggacc tctgtttcac ttgttggaaa gaatttttct tacttgctga ttggcttttc
     8221 ccttttgaaa tgatttcttg actagttagt tttcgtttga tttcgggatt cagatttgct
     8281 ctcacaatcc gctttggtgc ttcttttttc agcatcatat ctttctgagg ttgagctgct
     8341 ttatctattg gaatattcgc ccttcttttt ccctgacgat ttgttgcttc actcagtatc
     8401 ttgcgttttt cgtccatttt cgcatttctt cgttgtcgtt gtttttctcg caaatccact
     8461 cgatccgctt tttcttggaa cagtccgcgc ttgaattctt ctggtgcttt tttcgacttt
     8521 tcgattcctt tatggagatt gtatttcaaa ttcgttggcg tattccttac ctgatccttt
     8581 acttgtttag tcttatcttt caccaaagct tgggtatcca aaactttgcc gatttttctc
     8641 ccagccaaat tcattcggga ttgtttctta ttatttgaaa ctggattctc cttagttaag
     8701 tcagatagta tttcgtgatc gttcggtaat cgtgaattct ttcgtagaga agaaccggat
     8761 ggcttaaatt tggattttga ttgttccttt gccaacttat ttccgactgc tgcaccacca
     8821 ccaaaagcca aagctcgtcc cacatttcga ttgagttgcc gcaattttcg attcattaac
     8881 atttgcggtt ttcgcatgac gcgtcttccc attgactggc tatcattact ctggaggttg
     8941 aacatactca taatgtcgcc caatttgaaa tagataccgg caaaggtcac aatttgcaga
     9001 aaagcaacca tgaaaaatgg ataggttgcc gaaatattga aaaacatcgt ggagatgcta
     9061 aatgcagtcg taatcaccaa agtaacaccc gctcgcatca taatcgtatt gaataaacga
     9121 ataatcgcct tctttcctaa actttcataa gtcggcaaca tggacaataa gaaactaatt
     9181 ggcagaaaca tcgcaaagat aatgaagaga atttgcgaga acaacataat ccctgttaga
     9241 agaaaaacaa agaaggaaat gccaatattg aacaatacca aaaagaccac ggtccctaaa
     9301 cggttcatgg tcttggtaat actgagattc gcattgtcat tgtcttcaat ctctgccttg
     9361 accgcttctt cccgatcttt gcctttgttt tcatctggac tcacagatag aatcttgttg
     9421 acccggtctt caccaatttc ttctatgttg gaatcatcaa attgcagtag taaccaaggt
     9481 tgttggactt gaatcgaaaa caaactatcc cgaatcaaat ccacactatc ttttccttgg
     9541 ctttctgaat tgggtacaac gatctcggtt ccaagggtaa gggccgcttc actaacatct
     9601 gagctaaagt cattgatttt tgtgatatag gtaggcgcat aagcaatgaa cgaacccgat
     9661 agtagaaaaa tcatcacaaa attaatcacg gctcgaactg ctttcgtggt ttctcgtttt
     9721 aataatccgg tatacgccac atacaccccc agagctaaaa tcatcaataa tagaaaccca
     9781 aaataaaatc cggaggtctg gagtccattt tcagtaatac cagccaatgt ttggatattc
     9841 tttccaatac tttcagccgt atccgaaatg aaatctaatt tgtaagcctc ttgaaccaca
     9901 taacccgtgg cgttcgacaa atacaaactc accgtccaaa tgaaattagt aatggcataa
     9961 agaccataca tcacgctttt ccctaagcca tcaccccaat tccaaggcaa ccaatcccaa
    10021 gagctatcca caaagaaatc tagttgatag ttgttcagtg aatatttgga atatagattt
    10081 cctgcttcaa ctgtatcatc aactagccct gtcgcctcgg ctaccgtacc aactaaactc
    10141 aatagaacta tcagagtgag tatgctaata aaaacgattc ccacaatccg aaaaagtttc
    10201 tttttcatcc cctcactcca ttctctcttg aacaggtgga cgggtatcaa aggcatggaa
    10261 gagttcttca aagactggat gtacctgaac cacgcctacc cgaccataca aatcctgcat
    10321 caagcattgg ccattttcca aatctcgtag gcgtttttga ttgctttcat cttctcgatc
    10381 caagccaaag aattccaagg ttttcttgat ttcatgaata tcggtactgc gaaacgcaaa
    10441 cttcaacccg atattattct tcaagcgttc atccagtaaa tcatcactat tttgcgtgac
    10501 aaagtaaacc ccggcattca tagcccgccc agcacgaacg agcttattcg ataatgtttt
    10561 cccttgcgct acttgaagaa aactccacgc ctcatctaag tctacaattt tgaagatttc
    10621 tcgattacta tggataaagt ccaaggcaaa agtggaaatg acgattagca tggaaacact
    10681 aagcaattcc atggtggtat attcttcaaa agtggtttct gcatctggta aaaccaaatc
    10741 cgctacttga atgatattta actgcctatc taaactaatc gactgggtaa tcgaaccatc
    10801 tgagaaaagc aaatgggcaa aatcataatc ggtaaaggac tcgatgtgat ccgcgatatt
    10861 ttccgcaact ggtgagcctt cttttcgaag ctcattaatt acttgtaata atccccgttg
    10921 attgctttgg gtaaccgtcc gaatggcctt acgcaacact ggaaacttct ccccatctcg
    10981 actagaaatt cctgttaaaa aggtcagaat atcaattgct agactttcag aatccttctt
    11041 tttctccata atcacataag gatcaagtaa tccttgattt tttggcttac tagttaagtt
    11101 gagaatattg atttcatccg caatttctgg caaggtttct ttccagccac cccgttcccc
    11161 ttttggatcg acaatcacag cttgtccacc aaaaaggacg gcgtaataaa ctaagagatt
    11221 gttactaaag gactttccgc caccgagtga accgaggaat gccgctgcta gcgcattggt
    11281 aactgaatcc ttgacccctt gtgaggccaa tgccggtttc aggtaaacat tgcgccccgt
    11341 atctacgtta taaccaaagt aaatgccttc caactcccct aacatctgag tggcgccaaa
    11401 accaagtccg gcgagaaaat cagacgtaac atactggata taatcattca tgtatcgctt
    11461 actagaaggt aagaattctt catgtagccc catcatatcg ccaaaaggcc ggactagttt
    11521 tacattggta tcatcataaa aatctagcac ttcatcacac cggcgcttta gttcatctac
    11581 tgattcggca cttactcgaa ccacatagct cagtttgtac atggattctt tggactgatc
    11641 caaggtagtt tccaattcat ctacggaatc caatgcatct aagacattgg aatttgtttc
    11701 attgtcagat tgataggcat ggttgtccaa gtcttttaat tcctttttct tattgcgaac
    11761 ggtagcaagt gcctttttgt tcgtcacaat ctccacattc atactagtgt caattgggaa
    11821 tgtaaattgt tgttgctggt aatagaaaag ttctgaggag ggaaactcca tttcccccac
    11881 aattgtatta attgttaagt acgctacata tgattcatga ttctcatgtt ccatgcataa
    11941 gtaccggggc ttttcttcaa tcaaacaacg actgggacgt aataagtcgt aacgtttcac
    12001 caaagtttcc gacttcagct ttttctttgg tagctgaaat tcatattctt caaatggtgt
    12061 acccttttcg ccatagatat gttcaatcaa atacatcaga tcactcggtg tgactcgtct
    12121 gactttaaat cttcgagcca atttactttc cattagtttt tcaagcttgg tatatcggcg
    12181 aatttcttca ttggataagg aaacaaaatc gcccatcaaa tgatgattca ctccatagac
    12241 aaattcttga aagccggaga agaaagattt cttgagattc tttagattga cctcttcatc
    12301 tgtcgcaatc agtttgaagc caataaaaaa gcgataatca atctgactat cgccaatcat
    12361 ggatactaat gcctctgtct gtaaatcaat tcgttgtttc gctacttctg ttaatcgccc
    12421 ggtaatttcc ttttttgatc gttcttgcgt ggcacgaata ctactttctg tggcaatttg
    12481 taaggcatga atcttgcctt cacgattttg cgcaatcaat tgacgaaagt tatcgtggac
    12541 ttgatatttc tgttcaggtg acaaaaagga gtaattgtaa ggtattaact catagtaggc
    12601 aaaacattct ccctcttgat taaagacaag attattttca atatacttaa tcgggtacat
    12661 agttcacact ccttaccaga gtaatgggtt cgtcccatcg tttcttctgt tctttgactt
    12721 tctttcctgc gaaagtgacc ttaaatcgta agtgatagct aataaaagat cgtaaaaatc
    12781 ccaatggttt cttaccgtca aacgtttttt gcgacatgaa ccaagtaact gccaccggta
    12841 tacctaagta ttttaaaaag gccccctcta tcatagatag tggaggcaca tgggcaaata
    12901 cgatgacaaa cagcaaggat aaaacaaacc aagccatctg gttaaaagtc acaggaaaag
    12961 gcagttgaaa gtcattaatt gcataaatga ccttttcaac tgcccaaata ctggtgtagc
    13021 tttttatttt cttcatcaaa tcattccttt aaaaagaagg atagtttaga aatcaactac
    13081 ccttcaataa ttttgttaat atggatactc aaaaacacct cgactcgtta ccaaataatt
    13141 gcctccaatc tccaagtctc taccacacgc atcaaaatca atatacgtct tcaagtgttc
    13201 tggtacttct ccaaaatcac cagcttctac aatcaagaaa tatgctacat cactcatatc
    13261 atcgcaatct ggataataga taatatcatc cacatgctct accatctcct caaaagaatt
    13321 gaagaaagca tgttgaatct cagaagcaac ttcaccaact ggcgttcctt caagttcctc
    13381 cgctaagcca cacaaccgat taatttcctc aattggcgta tactcatcaa tctcaaaggg
    13441 aagttcataa tcatgaattg catattcctc gtattcgtca tttaacccaa ttcgttcttt
    13501 cacttcatca aaatcgacag gtggggtaaa ccacgccccc accaattcac cttcgttata
    13561 tttcccgagg ttcgcaatat aaacacgcat ctgttccatc gtgcttcccc cttacgcacc
    13621 gacaattcgg ttaaacaaac tgagtaagac atccttcacc ccagaagcat taaagaccaa
    13681 tccgaccgca atcaaagcaa tcaccaaaaa gccaatcagt ttggaaaact cccgcttaaa
    13741 tcctaaaaac aaaccaatga ccacaattgc cagtaagact aaggattggg cattggttaa
    13801 aaaccattga tatatggata ggtaagttcc tgatattata aatatctcaa acttttcccc
    13861 ccattcacac ggagcgtgcg actttcaccg cactccgcgt tccatcgtgt ttatattgaa
    13921 aagtatttat gtagtagacc tatatcaagt ttaattcttg attgccagct aatactctat
    13981 atttatttag tcttatgagc tggactttat ttagcttcaa tagagctata tatttttgta
    14041 ttgtttcttc tgttttagcg tgtattagac ggtgtaccgc ctcttttacc aaaataagat
    14101 ttttataatc gtcatttcca ccagctccag ttggtacaat atgatgacaa tgtatttcat
    14161 tagcttgttg aaattcttcc agagttatag cacattttcc atgttgagca gaaaatagcg
    14221 aaattcgatt gtctgcgtat tctattgtgc gattttccaa agataatttc attaactggt
    14281 acatcagtaa tttatcaaca cttagattgg tgtggataaa ttttcttccc tctagagtgt
    14341 agcaattcgc taaatacacc tttgccattg gttttctgtg ttggatatat ccaacaggga
    14401 aaataggttc gccctcgcca gcaacatacc gaagcatttt agatttacca tatcttgttg
    14461 cctcctttgt cgtaagttgt ctgcctgttt ttctcaatct gccccttttg tttgtaccta
    14521 atctgttagt gaagatagcc attacagctc tattcagttt catacaatcc agatttacat
    14581 tcgttgcaat ctgataataa ttttgcatac ctaataccat tgaattataa agtcttgttt
    14641 cttctaattc cgttttccct tttgagggag aagcaatttt ctttgcctgt gataccagct
    14701 tgtctttttg gtttttgaat tgtttatcac ttatatgtga tttaacaact aacttctttc
    14761 ttctcggtat cattttaatc ttgaaaccaa gaaagtcgga gtatcttttt ctaacattta
    14821 caattcttgt tttttcttgt gatacttcca attttaatcg ttgttcaatc cattgagtga
    14881 ttgctatttt ggctttttca gcactttcct tgtagcggca gaaaactcta aaatcatctg
    14941 cataacgaac catatacatt tcctttaact ttgttttctt catttctttg taagcgttgc
    15001 ttttacatgg gctaccgctt ttattgatat gaaccacata gttttttgta acaggatttt
    15061 cctgccattg gctttctacc caatggtcta actcattaag aacaatgttt gctagtagtg
    15121 gggatattat tcccccttgc ggagttcctt tctctggata tgttattgta ccgttagtca
    15181 ttttgattgg agcttttaat atccttttta aaatgaatat caagtgcttg tcccgtattc
    15241 ccatagccca tatctgtttt atcagcttgg aatggttcac gttgtcaaag aaacccttaa
    15301 tatcaaattc aatcacataa tgtagctggc tgatttgtag gcggttatag gttgctttta
    15361 ttgcattttc aactgaccta ttaggtctaa aaccatagct gttttcacta aatttagctt
    15421 cgcagattgg ttctaatact tgttttatac attgttgtat aagcctgtcc cacatacaag
    15481 gaatacctag aggtctggtt ttaccatttg gttttggtat ttcttttcgc ctcactggct
    15541 taggtcgata cccatgtgga ctgcctgcaa caatgtatct tacttttttg acaactgttt
    15601 ctgctggcaa tttgccaata tctttaatta ttgtaccgtc tgttcctgct gttttactgc
    15661 ctgcatttgc tttgatattt cgatatgcca gtaatatatt ttctctcgat aggataatat
    15721 ccataagatg agtgaaaata tctccattct tacttttggc atataaatca tcaaaggtgt
    15781 tttgcatttc gtaatattca gcgtgtctta aatcttctac acacaatgtt tcatttttat
    15841 ttttaggcat aaggcttcac tcctttactt gaaattgagt gagctttttt atcttactcg
    15901 taatccttat tgaaacaaca taatactttt ctaatatata cacgacttga gggcattcct
    15961 ccattctcat tacaagaatt tcttaggttc gccctctgct tttcagcatt agttcaactg
    16021 cttattgttc ttcgtcagtt tattctcttg atgagaaccc aatacctttc ctcgttccga
    16081 taatcttatc tatacatata tatccttagg tcactagcta taagcctgct ggcttgatac
    16141 cgcctgtaac gttatatggc atttcataac ctatattctt actttgccac accagctacg
    16201 atttctcgta cctttgcatt tctacaaagt tcaaatttag acccgtacat tcgctgtttc
    16261 gtcagttcct tatagaacat tctaaccata gatattttat agacccccga cctgttggat
    16321 acgctaccca cctctggata gttttcgata gaagatatat gttcatcaac tattacggta
    16381 tctttcagcc gtctttaccg agcttcaaac attctgagtt tcctcataca atgcttgtcg
    16441 gaggctcagg gcaggcgttt cagagcgtta cttcatcatt ccacctgtca gattatcagt
    16501 tctcctaata accaaacttt ttcgttcttt tattagtgcc ttagcttttc actaaggaac
    16561 gagtcgcaca taaattttgt ccgaaattca tcttgtcact ttcctttctt ttccattgct
    16621 cgatccgctg ctgcacttgc attcatcaag cacatcacag taacaccaag catactgcca
    16681 ccaaatacca aaaaaatatc ttttacaatt cccatgacga ttcctctttt catataatca
    16741 aattttcaat tgccatactt tgttgctcca aaatcttttt atgtcggtct gataagcgtg
    16801 cattctgtac catatcctgg atataggttg tccgattcaa tcgatccaac tccttagcca
    16861 ttttccacgt aggggcgact tgtcggccta gccaattcaa ggttcgttca atggtatacg
    16921 gttttggctc ggaagttaat tgaatctctt tccgattccg tccaataaaa cgcccccatt
    16981 gttcattcaa gggccagttg gttcgccttt taccttctac tttatccgca aatcgcaagt
    17041 aacgattgat aatggaaaac gtggtgctct caatgctacg accttttaac aaatcttgaa
    17101 tcgcatgaaa agcacggtca tttttcagtc gaatctcaaa acgatttttc gtctcagttt
    17161 ggtctaatgg aatccctaac ttgatatatt gttcatagtc tttttcatac aaacagaaat
    17221 aaacctcact cttgagactg ccgatatata aggtattgcc catgccgtcc ttttcatcag
    17281 cctttaaaag ttccccggaa cgataacttt taaaggtacg aaacaaggag atacattctt
    17341 ccttctgaca ttttttcgtt agctcaggta tatccaatag ccccactcga tcattgattg
    17401 ccaagtccaa gcgtttcatc acgccaccgg cctttagaca atcctcgaaa aagtcgtacc
    17461 agctacgctt ttgagcgagt aaaaaagttt caaattgtcg gcaacctcgc ccctttaatt
    17521 ctaaaagcac acctttatct tcttcatgag acaacatcac cacaatatca cccatgacat
    17581 actgttcttg ataggagtaa aaggcataat cttcatggag catatactca aatttcaaat
    17641 ggagaatttc ttgaataatt tttcgtgcat ccgttgtggg aaagcgaatc cttacatagt
    17701 caaacagtaa cgtcaaaggc aaatcgggat taaagctttc cagttggtca aaaattttcc
    17761 tcatggtttt tatagatgcg gttacgccac cattttctat ctgatttaag tacggccgac
    17821 taagtccaca agccgtagcc aatttatttt gtgaaacgcc gtattcgttg cgtcggtctt
    17881 ttaatagccg ataatctaaa ttccgttgtg ccaagttctc acctccaaat tcaaggtgtt
    17941 aactttttca aatgagttaa cacctataca aattacttac tatctttgat acaacaagca
    18001 ttcttaatgg ttgcttactt attttttgtg tttttgtacc cccctgttag atacgggggt
    18061 gagacatcat ggtcggctaa cgccgtccat gaacaggcac agcaccacgg aggatttctg
    18121 cttcgcgaat cctccgcggt cagcgcctaa cagtcagttg tgttggttta ggttactcat
    18181 ctgtaatacc atttacttcc aactctgctt tcatttctaa caagtattga cgaatatctg
    18241 gttcttttgc ttggtccgca atccgctcaa attctgcaat tacttgattt atttcaattc
    18301 gagcatccgt ctcctcattc attaaatatg gatcgccagc gactttgaca ccattcttgc
    18361 gaatatcaaa ttttaaccgt cgcaattgtt cttctaattc tgggtctgac tgaatctttc
    18421 caatcaccca ataaatttct ccaatcaact catcataaac catgtacatc caactctttt
    18481 aatcttctgg caatatcaaa aatgaccgga actgttacag agtttccggc ttgcttatac
    18541 aactgactat ctgaattgac ttgagaatct cgatcaaaag cccaatcggg aaagccctga
    18601 agtcgccaac actctctagg tgttagcttc cgaatttgat actgatccgt gacgatcgct
    18661 tgttggtctc ctgttagtaa ggtatgaaca ctctgttttc ccagacgacc tcggcgactt
    18721 gtggaattag gaaaagccag attcacactg tcttctggct tcacctcttg atattctgtt
    18781 tcattttttc gaatcatgat ttttgtacca ccttgtttcg ttgacaaggt gggggcggtt
    18841 ccagtaactt cataaactcg attaccttgt tcaaactttc ccggtaatag tcctgctaca
    18901 gagattccat cccttgctgg cagtgcgatt ttgattcctt catcacctgt taaggttggc
    18961 gctacgccat ctgaaccata gacttgtcca cccattcctc gattggaagg attgatattt
    19021 accaaatttc tgatgttttg atcagatgtt gaagacggtc caatgatagg aaatacttgt
    19081 tcggtacatg cgtctcgaga atgtccgata aggaatatcc gctcccggct ttggggaatg
    19141 taggcctttg agttaagcac ctaccactcc acgtcatacc ccaatccatc cagcgttcgg
    19201 aggatcgtct cgaacgtagc ccctccttcg tgattgagca atcctcggac gttctcaagg
    19261 aataaaagct taggtcggag aagagtggtg aaccgagcga tttcaaagaa taaagtacct
    19321 cgagtatcgg aaaatctttg ccgctttccc gcaattgaaa aagcttggca cggaaatccg
    19381 ccgcaaagga tgtccactgg tccgagggat tgaataaact catctgatac acttgtgatg
    19441 tcatgcattt ccacctcctt acttgtatca tgaattgctt tataactacg tctagcaaat
    19501 ttatcaattt cacaaaaacc gatacaatga tggctcgcct gttccatacc taaacgaaag
    19561 ccaccaatac cggcgaataa atctaaaaat ctcatacatt acctcattca ttgcaaagaa
    19621 aaaagccaag aacattttgc tcttggctca tacattatta atttgtccta ctatttttta
    19681 tttaatccaa ctaatttccg ttgtttttca ttggcctgat cggctacata tttaatcacc
    19741 gttttgaact cttttgcagt agcattttcc gataaatccg caatgctctt attgtatttc
    19801 tttacaattt tcgttacttg ttctttctca gtcatgtaat tgaacctcca aatctttcct
    19861 actataccct agtggtaaaa ataattctag attatccgta gcataatgaa cttcggaaat
    19921 attattcgta taatctttta ttatgatatg aagcaataca tctggatgtt tactctttac
    19981 ttttgcaatg ttctctcttg atttgaaaaa agcgttaata aatgcctttt tcggcacatg
    20041 acgtccctct gcaatttcac gttcttgtgt aaatttccaa gcgattttag ggtcttgata
    20101 aacatagaaa atcgtcactc gaaaatgttt tttcaacgcc cgcgagatat ttttttcagc
    20161 acttaatatt gcaaaagttg catccagaat aaaagaatag ccatgatcta agacatatct
    20221 aaatgcctgc tctaccaacc aagaagatgc tttttgaaag agactagagt tttttccatt
    20281 ataatcagga aactgggtgc gaaagtaatc cgcatcaata atcacatggt tatcataatt
    20341 ctcagctaat ccaatggcaa cctctgattt tccagcccca ggactccctg ccataaaaac
    20401 tgcgtctcgt ccaacttcgg gggatttccc tttgataata taagaaataa acttatcctt
    20461 atttgctttt gcatactcta aataagaatc attactttcc atattaatta tcccctttct
    20521 tcctactata taaaagtatt atacgttact tgaagtataa agtcagtatt gaagatcact
    20581 caatcattca tcttttctaa tttactaatt gaatctaaga aatcataccc ttttggtaca
    20641 agtggcgtat aaaactcact aatcacactg ccacctgtat ccacataacc tcgacctttg
    20701 atgtgcttca taaagaagtt tttttcgacc tccccaaaca tcatggaata tccgagttca
    20761 ctcatacgac ccaaagccac gcgaaagttg aattgatcgc ggattccgtc tcctaaatat
    20821 ttcgcatccg gcctttggca agccaaaatc agaaagaaac cagattgacg acccaacatc
    20881 acgatttgtt tgagcttatt caaaatcacc gcactttctt tcgtcgttaa catctccata
    20941 taagccacgt attcatcaaa gattaaaaag ttgggtggaa gtccaagata ggcataattc
    21001 tctcctgttt tgtagttaga catttctttc attgccttgc tacgagccat catacgttca
    21061 tagaaatctt ccacacaagc agaaatttct tccttttgtg aataaacatg aggcatcacc
    21121 gtacctaaat cagctaaatc tgcatttttg ggatcgagga taaacaattc tgcatctgac
    21181 ttcaatagtg cttcgataat ggtgaggagg aaataggtct tccctccacc cgtgccacca
    21241 gcgattaaca tatggggtaa agaatcataa gcccatactt gatttttcat caatcgcaga
    21301 gtcccatttt ctgccactac ttcgtctatc ccaattcgat tggcgatcat atcatacagt
    21361 aaagtatact ccacatagga gtctttgagt tccttttcta ctaactcaca atacagtccg
    21421 ctttctaact tcttttccaa ttttaaaagc tgatcttggt atttcccaag tgaaatttga
    21481 acgcgaatag acagtaatcc atctttcatt cgataacaga ttttggggaa gtaactgata
    21541 gtctctctgg ttcggctact attcaaatct ttgaaaaagc cttcactttg agtttgtttc
    21601 acttcatacc aatggttctc tagaatcatc cgagctagct tttgtcgatg ccacaagctt
    21661 cgccaatgat caagaaaaaa gtgaatataa agcaatatca tagctcctga aaaagcgaga
    21721 cttccaagca gacatactaa atcttttata gaccaaacga tttcaatagt agaaagtgat
    21781 agcttagaaa tatcaaattg tctaaactct tgaaaataaa agacgctcat ccaaccgatg
    21841 aacagagcga gtagccagcc aagaacaaag tgataaacca agtgctgatc tcctgcgcgt
    21901 atgcgatgtc ctttgtacat tttgatggtt tccacctcca atcgaaaaag cccaatagga
    21961 tttcattagg caatttcaaa aagttatgct agtcatttac tgctacgagg aggggagttg
    22021 tttcgtttca accaattgat ttaaaaagcc ttcaatagca tccggcatct ccacaccaat
    22081 ttcttccaaa atggattctg cctgccgaca taagttttca tttacttcga caactataaa
    22141 gaaagactct tcttctccta accagtctag tacatcagag tcttcttcac tgtcgctatt
    22201 ttctccaagt aaaccttgat gtataatcag aaacattttt tcatcagagg ttaatgcttt
    22261 aacgagttgt tcctcagtca gctcgccttt tccatattga tcgagacaat ctaaaatatg
    22321 ttcaacatta atttccataa tcttcaccta tttctttctt tcattttggt tcgtaggatt
    22381 tccttgattc ggctgccgct tcaagactaa atcatccgcc ttaatgaacc aattaacggt
    22441 agttccttct cgaaaaacat aattggcaac ggtatccacg attggattga ccaactctac
    22501 ctctgcatca taagcaaact cgcgtaaggg tacattagct gggatactaa cttgaatcat
    22561 tcgcccttgt gcagaggatt ttaaattata ggttcgttct tttacctcat taccaattgt
    22621 tccatcctca ttttgcttcc gtacttctcg gcgtaatgcg gaaaaacgca acttcccaaa
    22681 tgttaaacct gaatctacga caataccttc tgttaatctc atcttctttt cctcctatgc
    22741 tttcactaaa tcatcaacat acaacacgta atcagtaaac ccacgattct caatctcata
    22801 gcctttggca accaaatggg gattcacggc ttttagcggt tggtcttgat caaaatcctt
    22861 ttcaccggct tccgcagcta ctaccacctc aatatcatcg gcccgttgct tactggaata
    22921 caaatgataa ctgcgaccaa tcaccgtagt attccgtcca tatccttctg ttataatttc
    22981 tccttcaccg gcataactta actttccaaa ggtttcagcc atatctggca cgacaaattt
    23041 caattccatc ttcttttcct cttttcattg ttagttttgg atacaaaaat agcgcgacta
    23101 cttttttagg aggcggtcgc gcacgttggt tcttaggatt tacttgtatc atctcatcac
    23161 cttagttccg gcgattccag aaataatagc ccgccgtcgc aaaaatcaaa gtaaaaccaa
    23221 tgaacagtaa aacattggaa tttttctccc cggtttgagg gaaggtcttc actgtgggac
    23281 tagattcttg agagccactt ggaacagttg gtgtttccgg ttgtttcggc gtacttagta
    23341 tggttggaac ttcttttggt gttaaggttt gagatttttc tttcaaatct tcattgtgtt
    23401 ttccattgat attcccatct ttatcgtaat tgatttccgc aaaagtaaac ttggttcctt
    23461 ctggatattt tccggtatct acttttttcg caagcacagt tttggtgaat tctttatcat
    23521 ttacttcata atcaatcttg ccagatttcc aaatttcttt gtttgtacca tccggtaata
    23581 aagcatacag aattgtttcg aaagcttctt ttgagccatc cagtacatca tgagtaatcg
    23641 acacatcatc aaacatatcc accacatcac catgagtaaa ggtctgagaa ccgttttcta
    23701 ggtgggcctt tgtttggata gaaacatggt gttccactgt acaactgaca gtttgggctt
    23761 ggttattcaa gctaacatcc ttcgctactg gcttatcgcc tgcttcaaaa gcttccttat
    23821 tttcatacac atagttgaat aagacaatag ttttatttaa aacttgttcg gcggttaatt
    23881 tatgctgaat tttccaagtt cctgttttct ctttgttcgc caacgttgcg cttgtttctg
    23941 caatcaccac tggtttggct ttttcatctt tttcttgggc agcttttgtc gcatccacat
    24001 caatggcttg cgctaccaca taccagtctt ctttgatttc atgtagattg taactcaatt
    24061 tatcaactag ctcggtagaa tccagagaag ttaattcttt gtttccgtct ttccaagtcg
    24121 caagagaagt taaactatct tcttcctcag gcaaatcata gagcattaaa cggttcaaac
    24181 taacagaaaa tgctttgttc gtcagcttct cgtaaggaac ggtcaccgtt ttaattggtt
    24241 gtttttggtc ttgttcggta atggtaaaga catactcact cttcacaaag tcttctttgt
    24301 tttccttaaa agtagaacgg atttctaatg gcgtaatctt ttgaaaacct tctggggctt
    24361 ctacttcttc aagtaaataa tccccataag gaagattttc aaacttgcca taaccatcaa
    24421 accctaattg ctcgttataa gctgtggtcg cttcatcttc ggcacctgtg atttcattgg
    24481 tcccttccaa tggcgacact ttaaaggtca gatcattgaa gccagtttcg gtagtgcccg
    24541 cagctgatcc agcaaattta aagaaatcaa agccaaagcg aatcacttgt tcttttgccg
    24601 taacatttcg ggtaatcacg gcattttttt cgctatcatc cacctttttg atggatacag
    24661 gatactttgt ttcatcaagg ctatatcctt cgggcgcttt tgtttcttgc caataatact
    24721 cgttgatagc taaatgttta acggcaactt ggttcttttc gtctaaagtc aaagtgaccg
    24781 tttcatcaga acctttcgtt cccttcgcta actctggttt aaaagcctca ctccatttaa
    24841 cagcttttcc atccttagca gtaaacaagg tgtattcagc accctcaaag actgccttac
    24901 cttgtgcttt atccccagta tctttgtctt ctttcgtcaa agtggtttcg ccagtgactt
    24961 cttgattttg cccttttacg ttactggtaa caggggctac ggtttgattg gcatacttca
    25021 attcgacttt tacaggtttg aacgtattca cgaacccatg actagcttta gtttcggtca
    25081 cataataggt tcccaattcc aacgcattag caatctcttt tggtgtttcc gcatgaccgt
    25141 tttcatccgt ggtcatttct tgaacgattt caccggttgg actgtctttg cgaatggcaa
    25201 acgtattacc agctaaggaa taattgtcat tccaaagatc actccctgtt tcaactccgc
    25261 ttttatccag aatgatttgc cctttttccc gtgcgttttt tgaagtcacg taaatggttt
    25321 cgcctgcttt aatggtggta gtcatggggg tggtatcaat cgtataaggc gccggtactg
    25381 atttttcggt aatggttact tttgtaccat gaggaatctc atccaatgta gcaatgcctt
    25441 ctttgtcagt agtcacgtct tttgcaggta aagtttttcc aaagtctaaa tggaaaaccg
    25501 ttcctggtac aacatcaccc gattctttat cgactttttt gatctttaaa gaaccctccg
    25561 tttccacatc aattttgatt gtgtaggtat taggtttatc aatcgccccg gccattaaag
    25621 tctgttggcc agccttttta taggcgacag gtgttccggt accagcagat ttcttaagcg
    25681 tcagcacacc actttctttt gaattggcat ttggggtgat tatcaactga ttaccattca
    25741 cacgataatc aatgtttgcg gcattttcca caacctcatc gaactctgac aaattcagat
    25801 tattcgtatc cgtcacagta gtcgattgcc ctaatactgt tttagccgta ctattatgga
    25861 aggttggttt cttctgataa tcgtcaatgg cttgatttat ttttgcctca atggctgcga
    25921 tatttacggc actaccatct gatcgtttta ttgagtgaag ggtataccca ttaacttctt
    25981 gccagatcat cttctgtgcc acaatatgag tatctgcatc tgtacctact tttttccata
    26041 aaacggaaac taatttggct ttatcggaca tatctggtaa cgggtttttc tcatagccag
    26101 gagtgaactc attgtaaatc gggactcctg gttcaataca gaaaatatct tgtttaaagc
    26161 cgttgtacac tgcataatgc ggaacagtga cttctgacat tggacgacca tcactaaaag
    26221 ttccttcaac agcatattga tgtgctaaat ctagttctac ggttaccgtt tgtggatggg
    26281 taatttcatc agcaaaagta atcgtaggag caattgctcc aacaattgct tgaacagcca
    26341 aactcaaaat agttaataca cataatttct ttttttgttt tttatacatg aaatgttctc
    26401 ctttcaaatt aagcacaaaa
//
LOCUS       DAYYAJ010000028        26166 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000028 DAYYAJ010000000
VERSION     DAYYAJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.028"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..2849)
                     /gene="ileS"
                     /locus_tag="ACHATZ_000994"
     CDS             complement(51..2849)
                     /gene="ileS"
                     /locus_tag="ACHATZ_000994"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8403539.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(3111..3818)
                     /locus_tag="ACHATZ_000995"
     CDS             complement(3111..3818)
                     /locus_tag="ACHATZ_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8403540.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(3839..4621)
                     /locus_tag="ACHATZ_000996"
     CDS             complement(3839..4621)
                     /locus_tag="ACHATZ_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8403541.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(4670..4891)
                     /locus_tag="ACHATZ_000997"
     CDS             complement(4670..4891)
                     /locus_tag="ACHATZ_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8403542.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(4972..5571)
                     /locus_tag="ACHATZ_000998"
     CDS             complement(4972..5571)
                     /locus_tag="ACHATZ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8403543.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(5585..6262)
                     /locus_tag="ACHATZ_000999"
     CDS             complement(5585..6262)
                     /locus_tag="ACHATZ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8403544.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(6279..7520)
                     /gene="ftsZ"
                     /locus_tag="ACHATZ_001000"
     CDS             complement(6279..7520)
                     /gene="ftsZ"
                     /locus_tag="ACHATZ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8403545.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(7543..8868)
                     /gene="ftsA"
                     /locus_tag="ACHATZ_001001"
     CDS             complement(7543..8868)
                     /gene="ftsA"
                     /locus_tag="ACHATZ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8403546.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(9024..10244)
                     /locus_tag="ACHATZ_001002"
     CDS             complement(9024..10244)
                     /locus_tag="ACHATZ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8403547.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(10259..11347)
                     /gene="murG"
                     /locus_tag="ACHATZ_001003"
     CDS             complement(10259..11347)
                     /gene="murG"
                     /locus_tag="ACHATZ_001003"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8403548.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(11373..12734)
                     /gene="murD"
                     /locus_tag="ACHATZ_001004"
     CDS             complement(11373..12734)
                     /gene="murD"
                     /locus_tag="ACHATZ_001004"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8403549.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(12748..13710)
                     /gene="mraY"
                     /locus_tag="ACHATZ_001005"
     CDS             complement(12748..13710)
                     /gene="mraY"
                     /locus_tag="ACHATZ_001005"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8403550.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(13736..15928)
                     /locus_tag="ACHATZ_001006"
     CDS             complement(13736..15928)
                     /locus_tag="ACHATZ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8403551.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(15929..16330)
                     /gene="ftsL"
                     /locus_tag="ACHATZ_001007"
     CDS             complement(15929..16330)
                     /gene="ftsL"
                     /locus_tag="ACHATZ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8403552.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(16335..17294)
                     /gene="rsmH"
                     /locus_tag="ACHATZ_001008"
     CDS             complement(16335..17294)
                     /gene="rsmH"
                     /locus_tag="ACHATZ_001008"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8403553.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(17315..17746)
                     /gene="mraZ"
                     /locus_tag="ACHATZ_001009"
     CDS             complement(17315..17746)
                     /gene="mraZ"
                     /locus_tag="ACHATZ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8403554.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(17916..18284)
                     /locus_tag="ACHATZ_001010"
     CDS             complement(17916..18284)
                     /locus_tag="ACHATZ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8403555.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18455..19411
                     /locus_tag="ACHATZ_001011"
     CDS             18455..19411
                     /locus_tag="ACHATZ_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8403556.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            19484..19600
                     /locus_tag="ACHATZ_001012"
     CDS             19484..19600
                     /locus_tag="ACHATZ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8403557.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(19655..21337)
                     /gene="recN"
                     /locus_tag="ACHATZ_001013"
     CDS             complement(19655..21337)
                     /gene="recN"
                     /locus_tag="ACHATZ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8403558.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(21355..21804)
                     /locus_tag="ACHATZ_001014"
     CDS             complement(21355..21804)
                     /locus_tag="ACHATZ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8403559.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(21911..22729)
                     /locus_tag="ACHATZ_001015"
     CDS             complement(21911..22729)
                     /locus_tag="ACHATZ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8403560.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(22738..23628)
                     /locus_tag="ACHATZ_001016"
     CDS             complement(22738..23628)
                     /locus_tag="ACHATZ_001016"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8403561.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLTRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(23630..23854)
                     /locus_tag="ACHATZ_001017"
     CDS             complement(23630..23854)
                     /locus_tag="ACHATZ_001017"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8403562.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(23859..25196)
                     /gene="xseA"
                     /locus_tag="ACHATZ_001018"
     CDS             complement(23859..25196)
                     /gene="xseA"
                     /locus_tag="ACHATZ_001018"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8403563.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(25197..26051)
                     /locus_tag="ACHATZ_001019"
     CDS             complement(25197..26051)
                     /locus_tag="ACHATZ_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8403564.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
BASE COUNT         7089 a         5729 c         4403 g         8945 t
ORIGIN      
        1 gataacaaaa gggggtgtga caaagaacat gtcacacccc cttttccctt ttacttttct
       61 tcgaatcctt cagctacagc ttcaggatag aagtcttcaa cgatttttgc acaacgaccg
      121 catagatgag gaagtttttc atctactcct acatctttac gaacttggcg acagcgatcg
      181 caagtttccc cgtctgcttt ttccactgta atagcaacat catcaaatac catagcgtta
      241 tctggcactt gttcatctgc tgccataact tcaaagtagt ctggtgaaac gatcaataat
      301 tgtgcaatat ctgcatccaa agcttttaac atttcacgaa cttgttgttt tggataaaca
      361 gttagttttg cttccaaaga tttaccaatc aatttgctgt ttcttgcttc ttccaatgct
      421 tttaagacat tatcacggaa gtccatgaaa gcagtccagg tatccatcaa ttcttcttgg
      481 tttgcaaact cttcataacc aggaaactcg cttaactgaa cgtactcctc ttcttctttc
      541 aggaatgacc agatttcttc tgctgtatgc ggaatgatag gagtcaataa ttttgtcaaa
      601 gctacagctg tttgatagaa aacagtttgc atgcaacgac gttgataatc atcttctgct
      661 tcaatataaa ctacatcttt agcaaaatcc aggtagaatg acgatagatc aaccgtcaag
      721 aagttcataa ctgtacggta aatatgcatg aagttatatt tgtcgtaccc ttcttctcgg
      781 atttctttga ttacttggtt caatcgaacg gtcatatatt tgtctacaga acgtaaatca
      841 ttgtacgaaa cagtatcttt tttaggatcg aaatcttctg tattcgctaa taagaaacgc
      901 atcgtatttc tgattttacg atatacttct gccacttgac ttaagatatc catagaaaca
      961 cgaacatctg cttcataatc tacactagat acccataagc gaaggatatc tgcaccaaat
     1021 tgtttgatca ctttttctgg tgcaatgatg tttcctaaag atttactcat cttacggcct
     1081 tcaccatcta aagcaaatcc ttgagaaata actgatttgt atggtgctac accattgata
     1141 gccacactag tcgtgatact tgagttgaac cagccacgat attggtctga tccttctaaa
     1201 tacatatctg ctgggaatga taattctggt cgctgacgta aaacagcttc atgagaagaa
     1261 ccagaatcaa accagacatc catgatatct ttttcttttg taaattcgcc attaggagaa
     1321 cctgggtggg taaatccttc aggaagaagt tctttcgctt ctttttcaaa ccagataatt
     1381 gatccatgtt cagcaaataa ttttgccaca tgttcgattg tttctggtgt gatgattgct
     1441 tctccatttt cagcatagaa aatcggaagt ggtactcccc atgcacgctg acgggaaatt
     1501 acccaatcgc cgcggtcacg gatcatatta tacaaacgtg ttttacccca tgggataacc
     1561 cactctactt tttctacttc atccaaaata ttctgacgga atttatcgat agatgcaaac
     1621 cattgtggag ttgcccgata gatcacaggc tttttcgtcc gccaatcgtg aggataacta
     1681 tgagtaaaga aatctagctt cagcaaagct cctttttctt ttaatagatc agtgatcata
     1741 ggatttgctt tgtcataaaa gataccttca aatccaggtg cttcttctgt aaacaatcct
     1801 tttgagtcta caggtgacac tacagatagt ttatatttgc taccagcgat atagtcatct
     1861 tccccatgtc ctggagcagt atggactaat ccagtaccag cgtctaaagt aacatgatcg
     1921 cctaaaatca caagtgattc acgttcatag aatggatgac ttgcagtcat atattccatt
     1981 tcttgtccag caaattcttt tagaactgtt acattttccc aaccgatttc tgattggact
     2041 gtttctagca aatctttcgc tacgacaaac ttacggccat cggcttccac caatacatac
     2101 gtaaattttg gatttacagc aataccttgg tttgctggca atgtccatgg tgttgtcgtc
     2161 caaatgacaa aagctgtatc ttcatcaaga atatctttgc cgtctttgac attgaaagct
     2221 acaaaaatag atggagattt tacatcttta tattcaattt cagcttccgc taaagaagac
     2281 tcacttgacg gagaccagta aattggtttc aatcctttgt aaatatagct tttttcagcc
     2341 attttaccaa atacgcgtac ttgtgcagct tcataagaag gatctaatgt aacataagga
     2401 tgttcccaat ctcctgcaac tcctaggcgt ttgaaatctg ctcgttgctt atctacttgg
     2461 gaaagtgcat attcacgaca tttttccaag tattctgcca tggacatttc tttacgtttc
     2521 acgcctttat tagccaatac ttgttcaatc ggcaatccat gtgtatccca tcctggaaca
     2581 tatggtgcac gatagccaga cattgatttt gaacgaatga taatgtcttt actgatttta
     2641 ttcaatgaat gtcctaaatg gatattacca ttggcatatg gaggtccatc atgaagaaca
     2701 aagctaggtt ttccttcgtt caactcttgt cttttttggt agattcgatt ttcttcccat
     2761 tctttttgcc actcgccttc tcggtttggc aaattaccac gcatagggaa ttttgttttt
     2821 cctaattgca aggtttcttt cattttcatt caaccataca ctcctttatt tgttttccgg
     2881 acacaaaaaa gcatgttcag ccgcaaggga cgaacatgcc gtggtaccac ccaaatttag
     2941 ttgcatctat ctagtgcaaa ctctctgtga ccaataacga ggtcagccgc tgcttcttac
     3001 tctaatttcg gaaaagcagg tctcaagtag aggatctttc accatgcagt gtcttaccga
     3061 acttccactc tcatcggctc gctttaagaa taacaaagtg cttttctgtt ctactcgttt
     3121 tcattttcac caggcacgat cgagatatca atagcctgtg tatcaaatgc cgtcataggt
     3181 gcttctatga ctcctgtgtc tgagtttaca tcagtatcat tttcattgtc aagctgtttt
     3241 gctaaaactt ctttgatcac ttcatggctg tcagtcacgt agctggagaa tggttgtaaa
     3301 atttcatccc attccggact tttcacttgt tctaattgag actcaagcat caagctgata
     3361 cgttgatgga atactcgtgt ttttttctta agatcttcag tttctgtcgc aagctgtctt
     3421 gctttttctg ttgcatctgt caagattttt cttgcttttt cttctgcatc agttgttaat
     3481 tgatgtgcac gtttttcagc gttcgcaact aactcatctg ctttgttttg tgcagaagta
     3541 acgatcactt cggattcctt gcttgcactt gtttttactt tatcagccgt atcttgagct
     3601 acgatgattg attggttcaa agcatctttt aattcattga agtactcaag tttttcttca
     3661 gaatgtttga ctgctttttc taattctcga tttctttgag tcaattcctc atagtcacga
     3721 acaactaaat ctaagaaatc atctacttcg tcttgattat atccccgcat ttttgtcggg
     3781 aatgtcttat tttggatatc taatggagtt aaagccatga gtaaaccacc tttttatttt
     3841 atttacgtaa tacaccgaac agtaccctgt acttttcttt tttcgtcttc ccctcaagtt
     3901 cttggatctg gactctgcca taacctctga ctgaaataat atctaatagt tcaagtacaa
     3961 aatcaggtct tgtattttct gtccagttca cttttacttt accagcttcg atcagctgct
     4021 tggaccgctg tctggaaata ttataaatct cagagatcac actgtctagt cgcatggaac
     4081 taactgttcc tctttcttgt gtccagctat ctttcggcat aatcatttct gtgtatttga
     4141 ttggttccaa gcgtacagca acctttccaa tctttgttac ttgtgcagca acaaaatgat
     4201 ccacctcatt tgcaataaat acttgccagc gatcaccgtc cgagatgata tcgccaaagt
     4261 aagagcgttt gatccccgtt cccactaaag ttccaaggat tttcccatga cttaggctgg
     4321 caaatttgct aggataatgg atttcataca atgctgtttc aaaatcttcc tctacaggtt
     4381 cgtaataatc ggggaaaatc aaacatcttt ttctttctgc ctgttcatat ccgccataaa
     4441 acatgaaacg caaatcactg ctttgtcgaa tcagtgtttc taaaatatag gattgtcttg
     4501 ggtcaagaaa ttcggtcaga tagggtgcat attgtccttg aacctgttca atccaatcgc
     4561 ctaccgaatc gataaatgta cgttcatctg cccgaaagtg ctggtataca tttgcatcca
     4621 aaattttccc ctcctatcta ttctgagaat aaagattttg ttgcttcaat caataaacta
     4681 aatggacagt tatccatata acgatctgtg ctaaaccttg tacggctaag ttcaagatca
     4741 ggattgcccc taaaattgtg aaatccaagg gtccaatcct caaaggcaga cgatcgaaca
     4801 aactgatata cggttcgcag atcttagcta ataatcgtcc tagccctgat tgatacccgc
     4861 ctggaaacca agagagcaat gcatatacca tcaagataat gctatagaaa taggctgccc
     4921 gttgaagtaa atgaagtaat agtgcaatag tataaatcgt agttcctcct cttacagatc
     4981 aaacaaattc gcatctgcaa aagattgagc ggttgaactg tcaatctcca tcccagtcgg
     5041 tgtacaaaga aaaatctcat ctccaacacg tttgatatct ccatctagtg cataaactgt
     5101 tcctgttaga aaatcaacga ttcttctcgc ttgtttttct tctactaaat gaaaattcac
     5161 taaggttgct tctccagcaa tgattcgttt agcaatcgcc atagcttcag aataagctct
     5221 tggttccatt acagtgattt ttccagcaag atttgcttta ggttgtgtag aggttcttga
     5281 agctgttctt tctttcgcta cagtttttgt aggtgattga tgtagttcaa ttactttttt
     5341 cttttcagcc ggctgaatac tttcatgcgt tacaggacgt gaagtcgccg ttttttgttt
     5401 ttcatgagta ttcgcagcag atacagaatg agttgattga ttctgtgttg tatttttttt
     5461 agttggctgt acagcctttt ttgttgtttc aggataatct tggtattcgt actcttcctc
     5521 atcagctaat ccgaaaaaat tagcaattgc tcctctgttt aaaagcggca tttttgttcc
     5581 tcctttattc gtctttgaat agcgctgttc ctacacggat aaatgttgca ccttcttcaa
     5641 tcgcaatttg aaagtcttga ctcatcccca tacttaattc actacatggt gcatgggcat
     5701 aaccttttgc ttgaacaaga tcacgcaatt ctttcagcgt accaaacact ttatggattt
     5761 gctcatcaga agcttcttta ggcgccatag tcatcaaccc aacaaccttg atttttgtgc
     5821 aggcagctaa agattccaca aacggtaaaa gctcatctgg tgaaattcca tgttttgatt
     5881 cctcaccaga aacatttact tcaataaaac aggataattc tttctctaac cgcttctgga
     5941 tttcttctgc taatcttaaa ctgtccaacg catgaaagta atcaatttca tttacaatca
     6001 attttacctt tctgcgctgt aaattaccaa ttaaatgcca ctttatttca ggaaaatcaa
     6061 ataaatcttg tttcttttct aataatttat ctactcggtt ttctgcacaa ttcataacgc
     6121 cagctctaac caattcttca gcttgagcgg attcaacaga ttttgttaca gctactaatg
     6181 taacttcttt cggatcacga tgaacaagag cacacgaatc ctgaatttct tgcttgattt
     6241 tctgcaagtt gtcagcaatc atgtgctctt cctctccttt attttttacg acggaaaaac
     6301 ggtggcgtat ttaattcatc atcgccatga gattttgttt cttcgcggtt gaatgtatca
     6361 aattcttttt tttcgatatt gtcaaaattg gaatcatcaa ctctaggacg aacattttgt
     6421 tcacgtctga tatcccaatc gccaaaactg ttttcttctt ctgaagaagt tggtttagct
     6481 tgatccatat ctaatacagt cttttgagca ggtgattgca tttgtgcttg tctagctggt
     6541 cggctagact tgcgttcctt ttttgattca tcgatacctg ttgcgatgac cgtcacacgg
     6601 atttcatcgc ccatttcttc attgatagat gtacctagaa tgatatttac atcgccagtt
     6661 gctgcatttg ctacgatatc tgaagcatct tgtgcttcga acaatgtcat atccaaaccg
     6721 cctgtgatgt taagaagtac ttgttcagct ccatcaatag atgtttcaag caatggagag
     6781 gagattgctt ttttcgttgc ttctactacg cgatcttctc cactagccac accgatcccc
     6841 atcaatgctg taccttggtt ttccatcact gttttcacgt cagcaaagtc aaggttaacg
     6901 taacctggcg ctgtgatcaa atcagagata ccttgtacac cttgtcgtaa aacattatct
     6961 gcttcacgga aagcttcaag catcggtgtc tttttatcta caacttctaa taaacggtta
     7021 tttgagataa ttaataatgt atccacattt tctttcaaac gagcgatacc ttctgcagca
     7081 aaacggccac gtttcggtcc ttcaaaagtg aatggacgtg tcacgacacc aacagtcaat
     7141 gcgcccaatt ctttcgcgat acctgcaacg attggtgcag cacctgtacc ggttcctcca
     7201 cccattcctg cagtgatgaa aatcatatct gcaccgtcta aagcttcgcg taaggattgt
     7261 tcactttctt ctgccgcttt ttgtccaact tctggctgag aaccagcacc taaaccacga
     7321 gtatatttgg ggcctaattg gataactgtt tcagcttttg aattttttaa tgcttgcacg
     7381 tctgtgttgg ctgtaatgaa ttcaacacct ttaacgtttt cttcaatcat tcggtttaca
     7441 gcatttccac cgccaccacc gactccgatg actttgatta ctgcgccgtc attgatatta
     7501 ttatcaattg agaattccat atcttattcc tcccaggatg aattaatcaa aaatatttgt
     7561 aaagaaaccc ttgattttat tttttacatt ttctccggac tcttttggtt ccggctcatc
     7621 gtagattgtt tcttcaggtg cttcatagct ctcatatgtg tttgttgttt cttgttcaac
     7681 taccatttga cgagctgctg aggtttcacc cgtaacagca atttttgcca attgatacac
     7741 ttcactcaag tttgctgaat aatccacgat acttattaca tttgtaaata ccgggtttct
     7801 cagccccatg tgatttggaa catacaattt tacatttacg ccgaagattt cttgtgccaa
     7861 gtccacaaca ccaggaagac tagcagctcc gcctgtcaat acgacgccac ctggcaactc
     7921 taatgcttcg atttggtcca atgcatcttt tgctttgttg aaaatctgtt ccattcgagc
     7981 tgaaataatt tctgacaagt agcgttcatc gactttcact ggttctgact gaccaatcac
     8041 atctactgga aattcttcat ctggtgatgt ccgttctggg tatgcatccc cgtagtttat
     8101 tttcaacgct tctgcattgt taaatgacgt atttaataca atagagatat ctttagtgac
     8161 aaattcgcca ccttcttggt caagattggt aaacttcaat tgcttatcat gcataacagc
     8221 cgttgttgtt tggccgccac ccatatcaat gacgatcgta ccaaaatctt tttctccgtc
     8281 agaaagaatt gattcagtca gcgctaaagg agtaatcacc atttcgttga cgattaatcc
     8341 ggcattttcc acgcatttac gtatgttatg gatgatcgtt ttaggtcctg taaacaacag
     8401 accgtacatt tctaatctta cgccgatcat tccacgagga tctttgattc cctcgaaccc
     8461 atcgacagta aaatcttgtg gcaatattga cacgatttga cgttcaggag gtattgaacg
     8521 aacaagggca gctgaagcca catttctgac atcttcatcg gtgatttcct ttgaatctcc
     8581 gttaactgca atcattccct ggcaattttc tacttcaagc atgtttgctg gcaaaccgac
     8641 acttacgcct ttgatctgga tacctgcttt ttcttctgct tgtcgtactg ctcgttgtat
     8701 cgcttggacc gttttatcga tatcaacgat aatgcctcgg ttaatccctt ctgattttgc
     8761 gtttcctact ccaataatat tcatttggct gtcgatatat tcagccacga caactttaac
     8821 agatgtcgtt ccaatatcaa ggcctacata cattcctgtt tttgccatga atgggattcc
     8881 ctcctactta ctattccata tacaagcgaa caactagcgt ccgttttaaa atatacttat
     8941 taatagatat attcactgct tccaattcta ccataaatta ctggttttta gaaagtacat
     9001 tcttactttt taaagttgtt ttatcaattt tcatttaaac ttgacggagt tgtacttgga
     9061 ttatcttcta tttgacttgt ttcacttgaa tcatttgaat cagatgatcc gtttgtgcta
     9121 gacgatccag gtgtttgtgc tttttgcgtt tctgcctctt cacttgattc gtttgtttga
     9181 tccgcatttt cttcactttc agttgtttct tccgattggc ccgattcttc cgtttcttga
     9241 gtgctaccgt aaggatgcga gaagatacca acttccatgt cgatcacacc tttttcatcc
     9301 atatctttgg ctacttgcga ataatatttc atttggttcg caagattatt gatatttaca
     9361 ataacttgat tgccgtcatt catattcaat tggatcagat ttttattgct tgctcttggc
     9421 gtgtacttga tttcagaaat tgctttttgt aattctgatg ataactgatt gtattgcttt
     9481 gttaattctt tgatttttgc agagtctttg aactcttcca agatagggag attttttgta
     9541 ggatttgcta ctttttcatc taatactgtt ccattttcaa tcactgggta gtattgattg
     9601 ttttttgcga tcaaagcaat ctctttatat tctgttacat ccaaatcgaa ttcattcatc
     9661 cctttgaaat gtatatttgc tgtttcgatt cttggctgtg cttttttaag gcgatcgata
     9721 taagtagatc gatgaaaata ctgtccccaa acattttctc ctaatgacaa ttttgtatca
     9781 gagatgattt cttggctgtt gactgtctta ttgcctgata ctgcaactgc ttgtaatttg
     9841 ctataaggag aaacataata gatcaaaaaa atcagcggta tacccaatac agtgatgatc
     9901 aaagctaacc gacgaaatag tactttgtgc cgttgacttt ttaaattggg cagccggttc
     9961 aaaaaagaac cgttatatgg tccaccgttt ttcttttgct tgatctcttc aaaaaattcg
    10021 atttttttct cggactggct ttcttcttgc tgttcctctt gaaccgtttt ttcttcttca
    10081 gaatctgaaa ggggtgtatc taatgttatg tcttcttttt ttggctcttg gtttttcatc
    10141 tcttgttctg ccagatattt tcggttagcc ttttgccaag gagtcaaatt gttttcatca
    10201 gaagacggat gagacaattt ttttggttct tttttcttac tcactaagac caccgccttt
    10261 attttttgat tatttgtttt actaagtcat aaagacgttt tgaagcatca gggattcctt
    10321 gttctttaga agctcggcac atttgctttt gcaattcttc atcacccatg atttcgttga
    10381 ctgtctgact tagtgattca cctgttagtt cattatctgc aatcattttt gctgctcctg
    10441 cgtgaacaag actcattgca tttttagtct gatgatcatt cgtaacatat gggctgggaa
    10501 ccaacactgc cggtaacccc agagctgtga attctgcgat cgaagttgca ccagcacgcc
    10561 ccaccagcag atcactggaa gccatgacct ctgccatatt tttgatataa ggctggacac
    10621 taatattttg tatttcagga tttattttat ttaggatctc ttcataatat ctatctcctg
    10681 aagcatacaa aacttggtaa gaagaaggaa tatattcact taaaaaaact gatactgctt
    10741 gattgatttt aagtgctcct tgactaccgc caaaaatcaa gactgttttc ttttctggat
    10801 ccaaatcaaa tgttttcaaa atatctgatt ttttagtatc tgctacttct tgtgctcgag
    10861 gattacctac aagaactgct ttgttttcag gaaaaaagga agccgcatct ttaaatgaaa
    10921 gagcgatccg atccacatat cggcttaaaa atttattggt aacacctggt acactgtttt
    10981 gttcatggat aactgttgga atacccattt tggctgcagc gtacactacc gctccggaaa
    11041 catatcctcc agtaccgata acgatatctg gttgaaaatc actgaggatt ttttttgctt
    11101 gacggatact cttaagaaac aatcggaccg tcttgatgtt atgcatggaa atttttcgtt
    11161 taaatccttg aatctccaag gtatggaaag gcattcctgt atcagggacg attttgtttt
    11221 ctagacctcg ttttgctccc acatacatga attcagcatt cggttcttga gtctttacat
    11281 aattgacaaa ggcaagagct ggataaatat gtccccctgt tcctccgcct gtaaccaaaa
    11341 ttttcatttt ttatcgctcg cttctacttt ttctatttta atcggttcac agcttccatg
    11401 aatctgtttc ctctagtttc aaaatttggg tattgatccc agctggcatt cgctggtgat
    11461 aataaaacta catcaccatc tgtactgttt tctaatgcta aaggaactgc ttgttctaca
    11521 gaatcggctg ttactattgt ctcgatgcct gctttttttc cagcatccat caacttttct
    11581 tttgtttggc caaatacgac cattgctttg attcctttta atgaaggaat taattcatca
    11641 aatgtatttc ctctgtctaa tccgccagcc aataacacga ccttagacgt ttcaaatcca
    11701 cttagagcca ttttagtcgc taaaatattg gttgcttttg aatcattata aaattttctg
    11761 ccttggattt ctcctacata ttgcgtacga tgtggtacgc catggaaaaa atgcaacgtt
    11821 tctcgtatag cttcatttga tataccatac aattttgcta cagaaatagc agctagtgca
    11881 ttttctacat tatgcttacc tggtacgcca agttcagata tttccattat tttttctttt
    11941 ttatagtata ttgagtaatc gtcagcatac acaccgtctt ccaacacttc ttttgtcgag
    12001 aaaggcagta cgcgtgcttt tgttctttgc gctaattctt gtagttctga ctgattccag
    12061 ttaaggatca gataatcttt ttctgtcatg tttttttgaa ggttccattt agctttgaca
    12121 tattcttttc ttgtaccatg ataatcaata tgtgcttcat agatattggt aatcaccgct
    12181 atatgagggc ggaaatctgt gattcccatg agttggaagc tagacaattc catcacaatt
    12241 ttatcttctg attttgcttc ttgcgctaca ccacttgctg gatacccaat attacccgct
    12301 aaacgtgcaa ttccttgatc tgctcctgca ttcagtagta gtcctgtcat cgttgttgtc
    12361 gttgtttttc cgttcgttcc tgtaatccca ataattgggc attccgctac ttcgtaagct
    12421 agctcaactt ccgtaattac cggaataccc atttcttggg cttttgctac taaaggatga
    12481 ctgtagggaa tccctggatt tttgaccatc aatgaaaatt cttcatccaa caattcgatt
    12541 ggatgactac ctgttattac ttttatccct aaagaaagca gctcttgtgc ttccggattt
    12601 tcgtcaaaag gttttccatc attaacggtg acaagtgcgc ccaattcatg taataatttg
    12661 gccgcactta ctccgctttt tgcgagtcct aagacaagca ctttcttatt ttcatatgtt
    12721 gatatttttt tcatttcccg tcatctccta ccaaacgatc cataatgtga tcaacgaagt
    12781 cacaatgctg atcaaccaaa aaactgtatc aatcttccac tccgaccatc cgcacatttc
    12841 aaagtgatga tgaattggag acattttaaa aattcgtttt cctgtaagct taaaggaagt
    12901 aacttgaagc atcacacttg ctgtttccat aacatagatc aaaccaatca aaagcaatgt
    12961 ccattcttgg tttagcatta tagaaattgc cgctaaaagt ccgcccaacg ctaatgaacc
    13021 gacatctccc ataaaaatct tggcaggttt tctgttgtag acgaagaaac ccagtaaacc
    13081 tcctaaaaca cttaagcaaa tgattaatac atcatactga tgctgatgcc aagcaattag
    13141 accgtaagct gcaaaagaga tacttcctaa gccagcaact aatccatcga tgccatctgt
    13201 cagatttact gcatttgaaa atccaacaag ccagaagaga gcgaaaatac cataaatgat
    13261 tcctaaaggt agatctagtc cgaagaaatt caatacacct ggataacctt cagaacggta
    13321 aacaagataa aagatgattc cccctagaat ctgtccaatc aatttctgct tcgagttcag
    13381 tcccatgttt cttttcttga atatttttat aaaatcatca agaaagccga ttactccgta
    13441 taacgctaat acaaaaagca agatgaaaag agtaggagtt agctgttttt gccaagctcc
    13501 aacccaaatt cctgtcaaaa tagagccgat cagaaacact aagccgccca ttgtaggagt
    13561 tccggctttg ctattgtgcc actttggtcc ctcctcacga atcgcctgac cttgcttttt
    13621 catttgaaaa taaccaataa atagcggcat tgcggcaatt gtcattgcac aactgctgac
    13681 aatgggaatc aatgcttgtg tccattccat gctcttttct ctcctatttc tcttcttatt
    13741 cttctaaagt aaaactaagt ttgtcttcag tgattttttc atatggagca agttcttgtt
    13801 tgacacaata accatctcca tcaaaactaa cttcaatacc gaggattttc cccagcttca
    13861 tgatatctgc ttttgaccat cctgttacgt caggcatcag cttatcacca ccagtatata
    13921 ggatcagttt ttctccagag atcaactgat caccgttggc agtagattgt ttttgtactt
    13981 ttttaccatt accgatcact acaggttgca gtccactttt ttgagcatca gctgctgcaa
    14041 cgtccgcttc aagattacga taatcggcta cggatacttt ttctgttttt gtttctgtat
    14101 ctgaatcttc ttcagtttct ttaaaatcca ttgcccgttt catcaatgga tttgcgattt
    14161 tagccaatgc cattcgatca tatgtcttag ggtgtttcat tgttagatac aagacatagt
    14221 ctggatcttc agaaggaacc atctccacaa tcgaatacag gtaggctgta tctcctgttt
    14281 gatatccccc tgtatcagaa gcgatttggg ctgttccggt ttttgccgat acattgtatc
    14341 caggtacact atatacacca taagcactac cgtagttttc gctttcgaca acatctctca
    14401 tatattcacg gactttttct gttgtttctt ttgaaaaagg ttgccctaga acttctgttt
    14461 gagttgttcg ctcttctcca gtttggggat ccaccacttt gctgatatat cgaggttgaa
    14521 tcattgtacc gttattcgca atagaagtga aagctttcat catttggaaa ttcgtcacac
    14581 ctaccgcttg tccataagca ctcattgctc ggtcgacaat atttaatgta ggtaaagcac
    14641 cgtttacttc atcatctaat ccagaatgag tactttgtcc aaatcccagt ttttgtaaat
    14701 agttatacca tcgtccgcct aatcgctgtt ccagtatgac catcccgacg ttacttgacc
    14761 atgaaagagc ttgacgcatg gtcagcacac ctttttcacc aaagtcatgg tcattgatcg
    14821 tagcatcttc tacttggatt tttccagatt ggaaggtttc attctcgttg aattctcctt
    14881 cttcgatagc agctgcagta gtgaaaactt tcatggttga tcctggttca taactatctt
    14941 gtaccaagaa attacgccag atagcatctt ttccagtcaa tccttccatt gtttcaggat
    15001 tgaaagtagg tcgttgtccc attgccaaga tttctccggt ttttgctttc atcaggactg
    15061 ctgtcaattc ttctggctga tattcttcat ttacttgatc catcagtgtt tctaaataac
    15121 tttgtagtct gctgtctaaa gtcgtgtaga tatcttgtcc gtcgacagct ttttcttctt
    15181 ctgccactgt acctggcaga ggattttgaa aattatcttt ttgataaata atcttaccat
    15241 ctttaccgct caagatatca ttgtaagcag actccaaacc tagtttacca actaagccat
    15301 tctcatcttt gtctggaact gcgtatccaa taaaatgaga agaaaagacg ccatttggat
    15361 acatccgagc ctgatggtcg acaaaataaa gaccagcgac accttctttt ttcatgtccg
    15421 cttcgatgtt ttgcttggtt tcttgggaga tatttttccc atagctacca aattcaactt
    15481 gatacagctc tttttttgcg ccgtcctcta aaatattcag tgcatctttt ttgtctatag
    15541 aaagattctt atgcaggatt tctgctattt tatcaaaatt cttttcttca acatacagtt
    15601 ttttatcccc agaggtatat gtttttgaga gtatcgcttt aattgaataa gaggaggcat
    15661 cttctgcgag cgccacaccg ttacgatcat aaatcgttcc tctttttgct tttactactt
    15721 cacttccttg gtacaactgt tttgtttttt cagctaatga ggtaccagca acatgacctc
    15781 caataacgat atacgagaaa cggacggcaa ataaaaagaa cattccaata ctcgtagcaa
    15841 ataaaatgat acctactttt ttgcgattgt tcatcggatt tagatttttc ttttccataa
    15901 accgccgaat tttattttta agactcattt acttcacttt ccttaaattg tcgtcattaa
    15961 tagacaatcc tttttcttct gcgatttgtt taatccgttc agtacgagaa agttcgtttt
    16021 tttcttgttc taaactcgtt ttttcttgct ttttatcatt gatttctgtt tgtattgacg
    16081 tgatttcttt ttcagctccg ctgatatttg tgcgcagcat aactgttaaa acacataaag
    16141 caacaatagc aatcgccacg cttgccacag caattttttc caaagcagaa atctgcttta
    16201 atcgttttgc cggtgattgt ggaagaatga tttcttgatt cgaagaagaa tcaggcattt
    16261 gttgttccgg ctgatccacc aattcaggta aatcatattc gtaatcttgc attttcttta
    16321 attctgccat cattctactc ctctttcatt cttatttttt ctgcaatacg taactttgca
    16381 ctgcgtgatc gattgttttc tgttagctct tcctctgaag gaaggattgg tttacgagta
    16441 atgactttca gttctggttg gaattcgtca ggtatgacag gaagtcccgg tggtagatct
    16501 tgtaccgtac tatattcttt aaacatactt ttgacaattc tgtcttccaa tgaatggaat
    16561 gtgattacgc tgattcttcc attgactttc aataaccgga ttgcctgttc taaagaggcc
    16621 tcttcggcac cgagttcgtc atttactgca atccgcacag cttgaaaaat tctttttgca
    16681 ggatgcccgc cttttcttct tgcgggagct gggatgactt gtttaatgat ttcgacaagc
    16741 tcaccagttg tttggattgg ttttgttttt cgtactcgtt cgatttcacg agcaatttgt
    16801 ttggaaaatt tctcctcgcc atagcggaag aaaatcttta ctaattcatg gtaggaatat
    16861 tcattaatta cgtgataagc ggataaagga gctgactgat ccatgcgcat atccaacggt
    16921 gcatcttgat gataactaaa tccacgttct gcttcatcta gctgaggaga ggaaacccct
    16981 agatcataca aaatgccgtc taccttatct acatgctcac tcaagcgttc ttctaattct
    17041 ctgaagttcg ctttgataaa tgtgaccata ccttgttcga cataagaagc taaacgcagc
    17101 ttggcgtgat cgattgcttt ttgatcttgg tcaaacgcat acagatgccc tttagaactt
    17161 agttggctta ataaatattc actatgtccg gcaccgccga gtgtacagtc tacataaacg
    17221 ccatccggtt tgatgttcaa tccatctacc gtttctttca acatgactgt atagtgttga
    17281 aatgtttcag tcattagtca gcctcttcct attcttatag tccgaaatca atcatacttt
    17341 cagcaatttc gtcaaagttc tcttctgctg ctgcagaaaa atcttgccat cgtgtctcat
    17401 cccaaatttc aattcggtta gatacaccga tgactacgca accttttgtc aaagctgcat
    17461 aatttcttaa cgtgctgggt atattgattc gtccttgttt atcaagttcg cattcagtgg
    17521 cagccgaata gaagaatcgc acaaaagtac gtgcgtcttt tttagccaac ggcatttcgt
    17581 tcagtttagc ttccagattt tcccattcgc tcatcggata gccgaaaaga cagccatcca
    17641 atccgcgtgt cagaacaaac ttttcgccaa gttcttcacg aagttttgag ggcacgatta
    17701 aacgtccttt tgcatcaata ttatgttgga attcacccat caacattgaa agccccccat
    17761 tcctccacta actaaaatta gtctaccaca atcccccact ttctaccacc ttttctggga
    17821 aatccgggaa caactttttt tattcataaa cattttttta aaaatcaagc tgcaaaaagc
    17881 cttttaaaag actttttaca gcttttctat ttttttcata ggtaagaaag tatgttcaca
    17941 atgatcaaga cagcgtacaa gacgattgtc aacagaaaaa ccaaacgcca agtcatcttg
    18001 aaataacgcg gataaataat ttcttcataa taatatgctt ggaaaacagc aatacaaatt
    18061 cccattagca aaacagaaat aaagaaaaac ggtaagatag aaattgtccc tgtatttcta
    18121 gacaatgcat gcataccgcc ccacaaaaaa ggaatcgcca aatcaggggc ttttatttta
    18181 aaacgacgat ttaacgaaaa taattttaca agaaattggc aaccaaacaa aacgattacc
    18241 gggtaaatat accaaaacaa gataagtggt gtgaatgtgc ccatgaacgt aaactccttt
    18301 tttatcgttg cttcaagtat actgtatata ctttacacaa ctgtttcatt ttatttaaat
    18361 ttatgcagtt atattctata tcttacaaac acattccatg tattccaatt ccctatccgt
    18421 tataataaaa gaaacaggca acaggaggaa aatcatgatc aaatatttta cattagataa
    18481 tgagaaactg cgtaataccg gaaaggaaac tgaccaaacg atctggtatt gtgtagaacg
    18541 tcctaatcaa gaagagattc atcagctgac acatcaattt gaaattccgt cagattacat
    18601 cacttccata ttggacgatg cagaaaattc ccgtacagaa gaattcaatc aatcaaagtt
    18661 gacaaagcca gcgttattat tgttacaata tccttttgct aaaacaagtc ctagcggtta
    18721 ttttcaagtg gatacatacc cgctctctat tattttgacg cccaaaaaga agcttatcac
    18781 aattacgaat catgaaccac tgtttttaaa gaaagtcttt agccagacct ttccaaaaag
    18841 tgatatttcg cctaatctca atatcttctt tcaattcatt tggcaggtca cactctctta
    18901 caacagttat ttagctacgc ttcaatctca atgcgatact ttggaagcca aacttcaagt
    18961 atccactgaa aacaaacaac tttatcaaat catggatatc caaaaaagcc tcgtttattt
    19021 cagagaagcg acttctgcta atttagatgc attgaaagaa ctagcgcaaa ataaactttt
    19081 ccaagaaaat catgctttga aaaatcattt acgtgatgta ttggtggaaa cagagcaggc
    19141 aatgactact tctcgcattc aattaaaact agtagaacaa atgaatcaga ctttttcagc
    19201 aatcgtatcg aataatctga ataatattat gaaaatttta acttctttaa cgattatttt
    19261 aactataccg acaatcgtcg gcagtatcta tggaatgaac attaagttac ctattgctga
    19321 aagggatgat gcctttatat ggctagttct attaacatta tttatttgta tcgtaactac
    19381 ctattattta aaaaaaggga agtttttata aaaaagccct cttgtggaaa tggaaaaaag
    19441 attgtacact tgaatcaact aattaatgac gaggttgaaa acaatgcgtg aaaaaaaaca
    19501 aacaagtact tttgctaaag taacaaaatt tgtcatttgg acaatgctga ttttaacgat
    19561 tggatctgtt gtattgactg ctgtaatgag tgtcatgtaa aaataaaata tagctatcag
    19621 ctgctgaaaa ggacctgaac cactgcgagg tggctcaggt cctttttgcg agtttcaata
    19681 gttcttttgc atgttcgagt gtgagttttg taatctcact tccagccagc atacgtgcaa
    19741 tttcttcttc tctttctttt gttttcaact ctgaaacaga agtttgcgta cgtccatcaa
    19801 tcactgcttt cacaatataa tattgatagt cggcgactgc agcaacttga ggcagatgag
    19861 tgatgcacaa aacttgagag tatttcgata tttgagaaat tttatctgcg atggcctgtg
    19921 caactcttcc gctcacacct gtatccactt catcaaaaat aatacttgtc acaccttgct
    19981 cagaagaaaa aatagttttc aaagcaagca acatccgaga cagttcgcca ccagaagcga
    20041 ctttcaccaa aggcttcagt ggctctcctg gattggtcgt aatgtaaaat tcgatttggt
    20101 caaatccatc atgattcaat tgtctatttt cttcttttaa gaaacgtacc tcaaactcag
    20161 tgttttccat atacagactt ttcaattcat gcagaatgga tttttccaat tcttttgcga
    20221 tttttttacg cgcttcatga agatctgcgg cttgttgctg aagtagaagc tctttttgct
    20281 ctagttcctc tttcattttg tccaattgac cttctgaaaa atcagaagaa gctagctctt
    20341 cggtgatttc gtcatagtaa gccaatattg actcaatgga ttcgccgtat ttacgtttca
    20401 actgtcggat taattctaaa cgttgcgtaa cttcttctaa acgactctca tctaactcca
    20461 ataaatcaat ctgacgactc atatcgccga ttgcatcttg cagcaaatag taagcacttt
    20521 ggatattttc ataaattgct tcatactcca catctagatg ggcgatccct tgcagttctg
    20581 aaacggccaa gccaacacca tctacactgt tttgttcatt attcgtcagt gctccgtaac
    20641 ctgcagctaa tccatcagca attttttgat aattgctaag tttttctctt tcttctttta
    20701 atttctcttc ttctccgact tgcaaatcag cagcagctat ttcttcttgc tggaaatgga
    20761 gcatatctat tctctgaaca taggattttt cattttgctg cacttttcgt accctttttt
    20821 ccagctcacg ataagagata tattcttgct gataactctt tttcttctgt tggaaagcta
    20881 catctccaaa acgatccaat aaagcaagat gtgattcagg ctggagcagt tcctgatgtt
    20941 cgttttgtcc ttggatatct actaaataac tgccgattct tctcagatta gctaatgtga
    21001 taatatgacc attcactcga caaacatttt ttccagttaa ggacatatcc cgtcgaacaa
    21061 tcagattgtc ctcatctgtt tcgatcccta attcaaccat cagttcagag aatccttctt
    21121 gttttggtag ttcaaacaac ccttcaagca cacatttttc tgctccttga cgtatataat
    21181 cgcttgaacc tcttcctcct gctaacagac ctaaagcatc gataataatg gatttacccg
    21241 caccagtctc tccggtcagc gcggtcattc cttcgtgaaa agaaagccgc aattccggaa
    21301 taatcgcaaa gttagtaata ctgatttctt gtagcatcac attcgctcct caacttaaag
    21361 gtaatgcagg aagtctttct tcaataaaac tgcctggtct tcagttcgtg taatcatcaa
    21421 aacattatcg tcatcattga taatcgaaaa taattctttc ttgtaacgtt tatcaatcaa
    21481 atttgctgct gccgaagcat ttcccggcaa tgttttcaaa ataacaaatt tttccatttg
    21541 gtcaactgcc acgaaagcgt cttttaatag tttttccaat ttagtattta catcttcgtt
    21601 tgtttcagct ggtatgctat aacggtagcc gccatctgaa gccggtacct tgatcagttt
    21661 cagttccttt atatctctgg aaatcgtagc ttgagtaaca gatattcctt tgcttttcag
    21721 gatttcaaca aattcttcct gctttcggat atcttgctca ctcaacaatc gagtaatcaa
    21781 acgatgccga tctttttttc tcattacgtc ttcgctcctt agcatattct tcttaatcaa
    21841 tgatagctta tttccttggt ataataaaga agaatccgca tacttctcac ttgattttca
    21901 cagttacaat ttatttatgg tcaaactgtt cgtgtgcatt tgaaaccact tctgccatat
    21961 taatagctga attgattgtt ccgctttcgg gtactttcct caagtgagcc aagaactcga
    22021 tatttccttc tccccccgtg atcggagaat aacttaattc cagcacatca tatccacctt
    22081 gtgccgcaaa tgacaaaatg tcttctaaaa ctttttgatg ggtctgaggg tcacgaacaa
    22141 ttccgttttt ccctacagct tctctgcctg cttcgaattg gggtttgatt aaagcaatca
    22201 cttctccctc ttcttttaaa atagcatgta atggaggcag catcagtttt agtgaaataa
    22261 aagaaacatc gataacagca acttctggaa ttcctaaggt aaagtcttcg ggttttgcat
    22321 aacgaaaatt cactctttcc attacgacga ctctaggatc ttgtcttatt ttccaagcta
    22381 gctgattgta gccaacgtct aaagcataac tcatttttgc gccattttgc aaagctgcat
    22441 cagtaaatcc gccagtggat gcaccaatat ctaacaatgt ttttccttca acagatagat
    22501 caaatgcttt gagtgctttt tctaatttta atccccctcg agaaacataa ggaagttttt
    22561 tacctttgac tcttaattca ctactcactg gaattttttc tcccggttta tcaaaccgct
    22621 cgtttttttc gttgtaaatc aaaccagcca tcactgaacg tttggcttgt tctcttgttt
    22681 caaataatcc ttgtttgacc gctaatacat ctacgcgttc tttttccatt ctttccctca
    22741 tttctccaca tcaaattgct tgatcatctg gcgcatcagc ccgccatcaa aagaaaaaac
    22801 gtcctcttct aatttgtcta tgatcttgtt ggcggataac agctcaatct ccaacgcctc
    22861 acgtgtcttt tctaggccta ataaacgcgg gtaagtattt ttttccatac gttcatcttt
    22921 accagctgtt ttccctaatg ttttcgttgt accaatcaca tcgagtagat catctcttat
    22981 ctgaaaagct aaacctaaat gttcggctaa tcgttgaagt agcaataaga tttcttcagg
    23041 ttgcttcgct aatatgccac cagccagcaa ggcataacga gtcaaatgcc ctgtctttct
    23101 ttcatgaata aatgctaatt cttctaaaga aagctttttc gattcgccct cgatatctgc
    23161 agcttgccca gcaaccatac cttgtgatcc cgcacaaaca gctaattgct gaagcagcag
    23221 aagcttagga gaattaccta agtgtgccat actgattagc tggaatgctc ctgtaagcaa
    23281 accatctccg gccaatatag ctaatgcttc gccgaatact ttatgattgg taggttttcc
    23341 tctacgaaga tcatcattgt ccatcgcagg caaatcatcg tggatcaagg aatacgtatg
    23401 caccatttct aaagcagctg ctacttgata gacgggaaca tcaataggct catcaaagga
    23461 agcaacggcc gttaaaagta ataagggacg tattcttttc ccgcctgctt tcactgaata
    23521 actcatcgca tcatagagtc ctctatccgt tgtatgctca tctaaaaaag acaagatttc
    23581 tttttctaca gcaggtagat gaatagctga aaagtcagtt aatctcatct cagctttcct
    23641 cgtttccgtc aaaatcaact tcttcatttg attctgtcat cattttcgtc aaggtctctt
    23701 ctgctttagc taatgtatct tggcaatgtt tgcttaattc cattcctcgt ttaaacgaat
    23761 ctagagctga ttctaatgga acgtctcctt gttctaactg catgacaatt ttttctaatt
    23821 cttgcagtga ctcttcaaaa gttggatttt ccatattttt attctccttt gtctatctct
    23881 tgtacttgtc ctttaacgat tccatcttga taatggatcg tcagcaaatc cccttgatga
    23941 atctcggtga cactttttac tacttgatca tttaacgtcg tatagctgta gcctcttccc
    24001 atgattttca atggactcaa caaatcaaga gattgaacag cttgagcaaa acgctgctgc
    24061 tgttgtttca tatattgttg gattttctct tctaagcgtt tttctagata agcagtttct
    24121 tggcgagctg ctttaactcg ataaagcgga gccacttgtt ccaattggtg gcttaatgcg
    24181 atcgtttgtt tttctttcgc atgataaatt tgctgtgtag cttgatataa tcgctgtttc
    24241 aactggtcta atttgatggt ctgaccttca tacaatcttt caggctgccg aaatacatag
    24301 gaacgtcgag cacgctcgaa ccgttcttgt ttgcgctgga tctggcgtac aaacgcctgc
    24361 tgcaatctag cttgccgttc ttgaatgcgc atcaactctt ctgacaaaac aggaacagct
    24421 aattctgcag ctgctgttgg agtggcagcc cgaacatctg ctactaaatc agcgatcgtg
    24481 gtatctgttt catgtcctac agatgaaatc accggcgtat tagcttgata aatcgcccga
    24541 gcaacccgct cttcattaaa cggccataaa tcctcaatcg aaccaccccc acgaccgatg
    24601 atcattgtat cgaaattgcc catctcttct actctctgga tattcctgac aacattgtcc
    24661 gctgcttggt ttccttgtac aactgttggg aacaaaacca attggacaat tggatatctt
    24721 cttttcaccg tcgtgatgat atcgcggata accgctccgc ttggactggt aaggaccgcg
    24781 attcgctttg ggaaacgtgg cagcattttt ttaggccctt gaaaaaggcc ttccttcatc
    24841 agcttttctt ttcgttcttc caattcttga tataaagcac ctattccatc aggctccatg
    24901 tgctctacat agatttgata agaacctccc gcttcatata atgaaatacg cccaacgaca
    24961 aggactttca ttccttcttt tggctggaat cgtaattttt ggaatgctcc tttgaacata
    25021 atagcagata tttttgcatg gtcatctttt aagctgaaat attgatgggc attggcacgt
    25081 aaacggaaat tcgatatttc gcctgttaaa tatacgcgtt ctaaataagg atcggcatca
    25141 aattttcttt tcaaatactt cgttaatgta gtgacagtta aatattcttg tgccatctat
    25201 tcacctgctc caaaagcttc ggcagatttt acggtttgat acatcagcat cgtgatagtc
    25261 attggcccta caccctttgg cacaggagtg atccagcctg caataggctc cacttcatca
    25321 aatttcacat caccgatcag cttaccgtta gcatcacgat tcatccctac atctatcact
    25381 acagcacctg gttttacgaa ttcttttgtg acaaaatgtc cacgtcctat tgctacgact
    25441 aaaatatctg cttcttgtgc cagttcagcc agattttcgg ttttagaatg agcaacagta
    25501 acagttgcat ctgccatcaa gagtagctga gccatcggtt taccgacgat attgcttcgt
    25561 ccgataacta ctgctcgttt acccgccgga tcgatagaat aagcttcaaa cattttcatg
    25621 atgccgtatg gagtacaagg aatcttgtcc ggtttacccg caaggagtct gcctaaattc
    25681 atcggatgaa aaccgtctac gtctttttca gggcggatag ctaacaggat cttttcttca
    25741 tctatatgct ttggcaatgg taattggacc aatataccat gaaaactagg atcttgattg
    25801 tatttttcaa tttctgcaag tagttcgtct tcagaaatcg tctcaggata acgctctacc
    25861 aatgaatgga ttccgatttc ttttgctaca cgttctttat ttcttacata gatttggctg
    25921 gctggatttt cccctaccaa taaaacgact aatccgggat ggataccatt atctttcaat
    25981 ttttggattt tttcagcaat ctctgcctgc attttttcag ctaattcttt tccgttaatc
    26041 aattctgcca tctaaccact cccagatatt ttttgtgttt tcattctacc atagaatttt
    26101 cccgatttat aacaggttta tgatgattca tagaaaaaag ctaggactta cgccccagct
    26161 tctaat
//
LOCUS       DAYYAJ010000029        25358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000029 DAYYAJ010000000
VERSION     DAYYAJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.029"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHATZ_001020"
     CDS             complement(132..509)
                     /locus_tag="ACHATZ_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403565.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHATZ_001021"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHATZ_001021"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8403566.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHATZ_001022"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHATZ_001022"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8403567.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHATZ_001023"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHATZ_001023"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8403568.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHATZ_001024"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHATZ_001024"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8403569.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHATZ_001025"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHATZ_001025"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8403570.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHATZ_001026"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHATZ_001026"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8403571.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHATZ_001027"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHATZ_001027"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8403572.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHATZ_001028"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHATZ_001028"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8403573.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHATZ_001029"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHATZ_001029"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8403574.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHATZ_001030"
     CDS             10661..11575
                     /locus_tag="ACHATZ_001030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8403575.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHATZ_001031"
     CDS             complement(11708..13273)
                     /locus_tag="ACHATZ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403576.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHATZ_001032"
     CDS             complement(13266..14717)
                     /locus_tag="ACHATZ_001032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8403577.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHATZ_001033"
     CDS             complement(14731..15747)
                     /locus_tag="ACHATZ_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8403578.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHATZ_001034"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHATZ_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8403579.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHATZ_001035"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHATZ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8403580.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHATZ_001036"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHATZ_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8403581.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHATZ_001037"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHATZ_001037"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8403582.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHATZ_001038"
     CDS             21340..22764
                     /locus_tag="ACHATZ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8403583.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHATZ_001039"
     CDS             complement(22806..23063)
                     /locus_tag="ACHATZ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403584.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHATZ_001040"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHATZ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8403585.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHATZ_001041"
     CDS             24337..25314
                     /locus_tag="ACHATZ_001041"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8403586.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAYYAJ010000030        24640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000030 DAYYAJ010000000
VERSION     DAYYAJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.030"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..1103)
                     /locus_tag="ACHATZ_001042"
     CDS             complement(255..1103)
                     /locus_tag="ACHATZ_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8403587.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1327..3267)
                     /gene="thrS"
                     /locus_tag="ACHATZ_001043"
     CDS             complement(1327..3267)
                     /gene="thrS"
                     /locus_tag="ACHATZ_001043"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8403588.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3604..3783)
                     /locus_tag="ACHATZ_001044"
     CDS             complement(3604..3783)
                     /locus_tag="ACHATZ_001044"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8403589.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3830..5653)
                     /locus_tag="ACHATZ_001045"
     CDS             complement(3830..5653)
                     /locus_tag="ACHATZ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8403590.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5931..8210
                     /gene="helD"
                     /locus_tag="ACHATZ_001046"
     CDS             5931..8210
                     /gene="helD"
                     /locus_tag="ACHATZ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8403591.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8303..8950)
                     /locus_tag="ACHATZ_001047"
     CDS             complement(8303..8950)
                     /locus_tag="ACHATZ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403592.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9129..9848)
                     /locus_tag="ACHATZ_001048"
     CDS             complement(9129..9848)
                     /locus_tag="ACHATZ_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8403593.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9979..11130)
                     /locus_tag="ACHATZ_001049"
     CDS             complement(9979..11130)
                     /locus_tag="ACHATZ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8403594.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11347..12087)
                     /locus_tag="ACHATZ_001050"
     CDS             complement(11347..12087)
                     /locus_tag="ACHATZ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8403595.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12091..12414)
                     /gene="rsfS"
                     /locus_tag="ACHATZ_001051"
     CDS             complement(12091..12414)
                     /gene="rsfS"
                     /locus_tag="ACHATZ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8403596.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12471..13064)
                     /gene="yqeK"
                     /locus_tag="ACHATZ_001052"
     CDS             complement(12471..13064)
                     /gene="yqeK"
                     /locus_tag="ACHATZ_001052"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8403597.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13048..13698)
                     /locus_tag="ACHATZ_001053"
     CDS             complement(13048..13698)
                     /locus_tag="ACHATZ_001053"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8403598.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13708..14019)
                     /gene="yhbY"
                     /locus_tag="ACHATZ_001054"
     CDS             complement(13708..14019)
                     /gene="yhbY"
                     /locus_tag="ACHATZ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8403599.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14035..15144)
                     /gene="yqeH"
                     /locus_tag="ACHATZ_001055"
     CDS             complement(14035..15144)
                     /gene="yqeH"
                     /locus_tag="ACHATZ_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8403600.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15141..15668)
                     /locus_tag="ACHATZ_001056"
     CDS             complement(15141..15668)
                     /locus_tag="ACHATZ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8403601.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15848..16633)
                     /locus_tag="ACHATZ_001057"
     CDS             complement(15848..16633)
                     /locus_tag="ACHATZ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8403602.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16626..17495)
                     /gene="accD"
                     /locus_tag="ACHATZ_001058"
     CDS             complement(16626..17495)
                     /gene="accD"
                     /locus_tag="ACHATZ_001058"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8403603.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17496..18875)
                     /locus_tag="ACHATZ_001059"
     CDS             complement(17496..18875)
                     /locus_tag="ACHATZ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8403604.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18883..19302)
                     /gene="fabZ"
                     /locus_tag="ACHATZ_001060"
     CDS             complement(18883..19302)
                     /gene="fabZ"
                     /locus_tag="ACHATZ_001060"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8403605.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19327..19803)
                     /gene="accB"
                     /locus_tag="ACHATZ_001061"
     CDS             complement(19327..19803)
                     /gene="accB"
                     /locus_tag="ACHATZ_001061"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8403606.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19806..21041)
                     /gene="fabF"
                     /locus_tag="ACHATZ_001062"
     CDS             complement(19806..21041)
                     /gene="fabF"
                     /locus_tag="ACHATZ_001062"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8403607.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21060..21797)
                     /gene="fabG"
                     /locus_tag="ACHATZ_001063"
     CDS             complement(21060..21797)
                     /gene="fabG"
                     /locus_tag="ACHATZ_001063"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8403608.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21809..22726)
                     /gene="fabD"
                     /locus_tag="ACHATZ_001064"
     CDS             complement(21809..22726)
                     /gene="fabD"
                     /locus_tag="ACHATZ_001064"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8403609.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22833..23057)
                     /locus_tag="ACHATZ_001065"
     CDS             complement(22833..23057)
                     /locus_tag="ACHATZ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8403610.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23110..24072)
                     /locus_tag="ACHATZ_001066"
     CDS             complement(23110..24072)
                     /locus_tag="ACHATZ_001066"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8403611.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24082..24522)
                     /locus_tag="ACHATZ_001067"
     CDS             complement(24082..24522)
                     /locus_tag="ACHATZ_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8403612.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6769 a         5465 c         4274 g         8132 t
ORIGIN      
        1 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
       61 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
      121 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
      181 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
      241 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
      301 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
      361 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
      421 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
      481 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
      541 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
      601 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
      661 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
      721 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
      781 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
      841 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
      901 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
      961 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     1021 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     1081 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     1141 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     1201 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     1261 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     1321 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     1381 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     1441 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     1501 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     1561 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     1621 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     1681 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     1741 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     1801 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     1861 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     1921 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     1981 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     2041 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     2101 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     2161 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     2221 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     2281 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     2341 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     2401 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     2461 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     2521 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     2581 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     2641 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     2701 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     2761 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     2821 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     2881 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     2941 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     3001 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     3061 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     3121 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     3181 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     3241 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     3301 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     3361 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     3421 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     3481 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     3541 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     3601 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     3661 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     3721 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     3781 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     3841 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     3901 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     3961 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     4021 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     4081 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     4141 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     4201 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     4261 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     4321 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     4381 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     4441 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     4501 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     4561 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     4621 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     4681 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     4741 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     4801 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     4861 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     4921 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     4981 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     5041 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     5101 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     5161 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     5221 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     5281 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     5341 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     5401 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     5461 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     5521 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     5581 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     5641 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     5701 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     5761 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     5821 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     5881 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     5941 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     6001 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     6061 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     6121 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     6181 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     6241 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     6301 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     6361 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     6421 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     6481 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     6541 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     6601 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     6661 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     6721 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     6781 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     6841 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     6901 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     6961 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     7021 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     7081 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     7141 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     7201 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     7261 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     7321 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     7381 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     7441 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     7501 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     7561 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     7621 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
     7681 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
     7741 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
     7801 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
     7861 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
     7921 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
     7981 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
     8041 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
     8101 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
     8161 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
     8221 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
     8281 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
     8341 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
     8401 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
     8461 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
     8521 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
     8581 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
     8641 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
     8701 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
     8761 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
     8821 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
     8881 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
     8941 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
     9001 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
     9061 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
     9121 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
     9181 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
     9241 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
     9301 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
     9361 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
     9421 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
     9481 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
     9541 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
     9601 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
     9661 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
     9721 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
     9781 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
     9841 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
     9901 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
     9961 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    10021 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    10081 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    10141 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    10201 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    10261 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    10321 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    10381 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    10441 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    10501 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    10561 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    10621 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    10681 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    10741 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    10801 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    10861 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    10921 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    10981 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    11041 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    11101 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    11161 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    11221 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    11281 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    11341 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    11401 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    11461 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    11521 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    11581 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    11641 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    11701 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    11761 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    11821 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    11881 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    11941 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    12001 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    12061 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    12121 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    12181 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    12241 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    12301 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    12361 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    12421 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    12481 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    12541 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    12601 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    12661 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    12721 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    12781 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    12841 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    12901 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    12961 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    13021 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    13081 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    13141 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    13201 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    13261 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    13321 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    13381 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    13441 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    13501 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    13561 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    13621 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    13681 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    13741 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    13801 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    13861 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    13921 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    13981 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    14041 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    14101 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    14161 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    14221 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    14281 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    14341 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    14401 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    14461 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    14521 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    14581 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    14641 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    14701 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    14761 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    14821 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    14881 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    14941 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    15001 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    15061 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    15121 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    15181 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    15241 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    15301 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    15361 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    15421 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    15481 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    15541 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    15601 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    15661 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    15721 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    15781 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    15841 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    15901 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    15961 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    16021 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    16081 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    16141 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    16201 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    16261 cctctccttc gccgcgctct tctgctccta caccgcaata agcacctgct gtattgatca
    16321 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    16381 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    16441 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    16501 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    16561 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    16621 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    16681 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    16741 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    16801 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    16861 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    16921 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    16981 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    17041 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    17101 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    17161 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    17221 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    17281 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    17341 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    17401 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    17461 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    17521 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    17581 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    17641 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    17701 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    17761 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    17821 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    17881 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    17941 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    18001 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    18061 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    18121 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    18181 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    18241 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    18301 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    18361 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    18421 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    18481 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    18541 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    18601 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    18661 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    18721 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    18781 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    18841 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    18901 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    18961 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    19021 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    19081 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    19141 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    19201 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    19261 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    19321 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    19381 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    19441 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    19501 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    19561 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    19621 ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    19681 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    19741 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    19801 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    19861 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    19921 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    19981 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    20041 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    20101 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    20161 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    20221 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    20281 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    20341 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    20401 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    20461 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    20521 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    20581 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    20641 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    20701 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    20761 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    20821 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    20881 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    20941 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    21001 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    21061 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    21121 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    21181 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    21241 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    21301 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    21361 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    21421 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    21481 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    21541 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    21601 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    21661 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    21721 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    21781 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    21841 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    21901 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    21961 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    22021 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    22081 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    22141 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    22201 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    22261 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    22321 tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat
    22381 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    22441 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    22501 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    22561 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    22621 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    22681 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    22741 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    22801 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    22861 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    22921 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    22981 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    23041 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    23101 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    23161 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    23221 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    23281 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    23341 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    23401 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    23461 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    23521 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    23581 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    23641 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    23701 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    23761 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    23821 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    23881 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    23941 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    24001 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    24061 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    24121 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    24181 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    24241 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    24301 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    24361 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    24421 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    24481 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    24541 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    24601 ccttaaaaga tcattttttt actatatgag ctttttttaa
//
LOCUS       DAYYAJ010000031        24260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000031 DAYYAJ010000000
VERSION     DAYYAJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.031"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHATZ_001068"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHATZ_001068"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8403613.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHATZ_001069"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHATZ_001069"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8403614.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHATZ_001070"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHATZ_001070"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8403615.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHATZ_001071"
     CDS             complement(3373..4680)
                     /locus_tag="ACHATZ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8403616.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHATZ_001072"
     CDS             complement(4683..5264)
                     /locus_tag="ACHATZ_001072"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8403617.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHATZ_001073"
     CDS             complement(5836..7128)
                     /locus_tag="ACHATZ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8403618.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHATZ_001074"
     CDS             complement(7125..7748)
                     /locus_tag="ACHATZ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403619.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHATZ_001075"
     CDS             complement(7751..8587)
                     /locus_tag="ACHATZ_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8403620.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHATZ_001076"
     CDS             complement(8604..9491)
                     /locus_tag="ACHATZ_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8403621.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHATZ_001077"
     CDS             complement(9507..10790)
                     /locus_tag="ACHATZ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8403622.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHATZ_001078"
     CDS             complement(11473..12156)
                     /locus_tag="ACHATZ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8403623.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHATZ_001079"
     CDS             complement(12174..12791)
                     /locus_tag="ACHATZ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8403624.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHATZ_001080"
     CDS             complement(12801..13625)
                     /locus_tag="ACHATZ_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8403625.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHATZ_001081"
     CDS             complement(13618..14178)
                     /locus_tag="ACHATZ_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403626.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHATZ_001082"
     CDS             14352..15308
                     /locus_tag="ACHATZ_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8403627.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHATZ_001083"
     CDS             complement(15613..16569)
                     /locus_tag="ACHATZ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8403628.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHATZ_001084"
     CDS             16673..17275
                     /locus_tag="ACHATZ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8403629.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHATZ_001085"
     CDS             complement(17363..18862)
                     /locus_tag="ACHATZ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8403630.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHATZ_001086"
     CDS             complement(18878..19402)
                     /locus_tag="ACHATZ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8403631.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHATZ_001087"
     CDS             19700..20449
                     /locus_tag="ACHATZ_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8403632.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHATZ_001088"
     CDS             20440..21225
                     /locus_tag="ACHATZ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8403633.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHATZ_001089"
     CDS             21194..21910
                     /locus_tag="ACHATZ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8403634.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHATZ_001090"
     CDS             complement(22012..23031)
                     /locus_tag="ACHATZ_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8403635.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHATZ_001091"
     CDS             complement(23050..24051)
                     /locus_tag="ACHATZ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8403636.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DAYYAJ010000032        24170 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000032 DAYYAJ010000000
VERSION     DAYYAJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.032"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..856
                     /gene="purR"
                     /locus_tag="ACHATZ_001092"
     CDS             38..856
                     /gene="purR"
                     /locus_tag="ACHATZ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8403637.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1359..2732
                     /gene="glmU"
                     /locus_tag="ACHATZ_001093"
     CDS             1359..2732
                     /gene="glmU"
                     /locus_tag="ACHATZ_001093"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8403638.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2826..3797
                     /locus_tag="ACHATZ_001094"
     CDS             2826..3797
                     /locus_tag="ACHATZ_001094"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8403639.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3803..3905
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3971..4540
                     /gene="thiT"
                     /locus_tag="ACHATZ_001095"
     CDS             3971..4540
                     /gene="thiT"
                     /locus_tag="ACHATZ_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8403640.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4621..5394)
                     /locus_tag="ACHATZ_001096"
     CDS             complement(4621..5394)
                     /locus_tag="ACHATZ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403641.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5661..7361
                     /locus_tag="ACHATZ_001097"
     CDS             5661..7361
                     /locus_tag="ACHATZ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8403642.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7331..7549
                     /locus_tag="ACHATZ_001098"
     CDS             7331..7549
                     /locus_tag="ACHATZ_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8403643.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7569..8459
                     /locus_tag="ACHATZ_001099"
     CDS             7569..8459
                     /locus_tag="ACHATZ_001099"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8403644.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8477..8761
                     /locus_tag="ACHATZ_001100"
     CDS             8477..8761
                     /locus_tag="ACHATZ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403645.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9248..9862
                     /gene="gmk"
                     /locus_tag="ACHATZ_001101"
     CDS             9248..9862
                     /gene="gmk"
                     /locus_tag="ACHATZ_001101"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8403646.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9867..10172
                     /gene="rpoZ"
                     /locus_tag="ACHATZ_001102"
     CDS             9867..10172
                     /gene="rpoZ"
                     /locus_tag="ACHATZ_001102"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8403647.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10425..12833
                     /gene="priA"
                     /locus_tag="ACHATZ_001103"
     CDS             10425..12833
                     /gene="priA"
                     /locus_tag="ACHATZ_001103"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8403648.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12848..13339
                     /gene="def"
                     /locus_tag="ACHATZ_001104"
     CDS             12848..13339
                     /gene="def"
                     /locus_tag="ACHATZ_001104"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8403649.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13332..14270
                     /gene="fmt"
                     /locus_tag="ACHATZ_001105"
     CDS             13332..14270
                     /gene="fmt"
                     /locus_tag="ACHATZ_001105"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8403650.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14270..15628
                     /gene="rsmB"
                     /locus_tag="ACHATZ_001106"
     CDS             14270..15628
                     /gene="rsmB"
                     /locus_tag="ACHATZ_001106"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8403651.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15641..16381
                     /locus_tag="ACHATZ_001107"
     CDS             15641..16381
                     /locus_tag="ACHATZ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8403652.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16378..18447
                     /gene="pknB"
                     /locus_tag="ACHATZ_001108"
     CDS             16378..18447
                     /gene="pknB"
                     /locus_tag="ACHATZ_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8403653.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18612..19511
                     /gene="rsgA"
                     /locus_tag="ACHATZ_001109"
     CDS             18612..19511
                     /gene="rsgA"
                     /locus_tag="ACHATZ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8403654.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19524..20174
                     /gene="rpe"
                     /locus_tag="ACHATZ_001110"
     CDS             19524..20174
                     /gene="rpe"
                     /locus_tag="ACHATZ_001110"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8403655.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20175..20813
                     /locus_tag="ACHATZ_001111"
     CDS             20175..20813
                     /locus_tag="ACHATZ_001111"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8403656.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20968..21822
                     /gene="mreC"
                     /locus_tag="ACHATZ_001112"
     CDS             20968..21822
                     /gene="mreC"
                     /locus_tag="ACHATZ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8403657.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21826..22335
                     /gene="mreD"
                     /locus_tag="ACHATZ_001113"
     CDS             21826..22335
                     /gene="mreD"
                     /locus_tag="ACHATZ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8403658.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22590..24164
                     /locus_tag="ACHATZ_001114"
     CDS             22590..24164
                     /locus_tag="ACHATZ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8403659.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         8122 a         4392 c         5199 g         6457 t
ORIGIN      
        1 gtaaaacgat aaaatttctt aaaaggagtt ggaataggtg aaagtacgtc gcagtgaacg
       61 tctagttgac atgacacagt atttattgaa tcacccgcat gaattgattt ccttaacaag
      121 ctttgccgaa cgttatgaat ctgctaaatc ttctatcagt gaagatttgg gaatcatcaa
      181 gaaaacattt aaagaacgag ggtatggaac cttggaaaca atcccgggtg ctgcaggtgg
      241 tgtattgttc atccctgaaa tttcatatga agaggcgaaa aaatatattg aatctctagc
      301 agaacgtcta tcagaacaag accgtctgct tccaggagga tatgtttatc tctctgattt
      361 gttaggagaa cctgaactgc tgcgtcaagt tggaagaatc attgcttcta aataccttgg
      421 tgaaaaaatc gatgctgtaa tgactgtagc cacaaaaggc gtacctattg cccaagctgt
      481 gtcctattat ctgaatgcgc cattcgttat tgtacgccgg gactcaaaaa taacagaagg
      541 atcaacagta agtgtcaact atgtgtctgg atcttctgaa agaattgaaa agatggaact
      601 gtctaagaga agtttgaaac gtggttcacg tgtccttgta gtggatgatt tcatgaaagg
      661 cggcggaaca gtcaacggaa tgaagagctt gattgaagaa ttcgaatcag aacttgttgg
      721 catcactgta tttgccgaat caaaattcaa cggtcatcgc gccatcgatg attatacgtc
      781 actgctttat gtcaaagatg tagatactca aacgaaaaat attacggttg tgccaggaaa
      841 ttatttttca gaataagagg ttgtgaaaaa gccgcttagt ttcgagtagt aagaaaaatt
      901 ttgtgaaaat ggctttccac attttttcat aaatttggct taataccgag aaactggctt
      961 ttgaacaccg tttatctaga ggttgtgaaa tcagcgtcct gtcctgagta ttagctcaat
     1021 aaacggaaaa attgctcctc atattttgcc gtttattgag ctaataccga aggtcagctg
     1081 attgaaaaac gtttaatgaa gaggttgtaa gcctaccgtt taactctaag tcacaagaaa
     1141 cgatttcaaa aaacagcttt tcatgtttta tgagtagtgt gacttgtgac cgaggagttg
     1201 gcaggagagc accggttacc aaatagatag gcccgttatg gtctatctat ttttttatcc
     1261 agaaaacgct gggataaaag aaaatcactt gttttgttga aaaaatgggt atactgggat
     1321 aaactgaaat cagcaaacta tatttaggag tgacgcagtt ggacgcacgt tatgcaatta
     1381 ttttggcggc agggaaaggt acccgcatga agtcaaaatt atataaagtt ttacatcccg
     1441 tttctggtaa acctatggta gaacatatca ttaatcgggt cagtgaaacg aaaccagatg
     1501 aagtgattac gatcgttgga cacggagcag aacaagttaa agcacaacta ggagaacgca
     1561 gtaaatatgc gttacaagca gaacaattgg gaacaggaca tgctgttttg caagctgctt
     1621 ctttcttgga aggaaaaaaa ggaacaacgt tagttatcag tggcgataca ccattattga
     1681 ctactgaaac gctgaacaat ctttttgaat atcaccaagg aaaaaatgca agtgcaacga
     1741 tcttgacggc acaagcagaa aacccaacag gatatggacg aatcatccgt gaccatattg
     1801 ggatcgtaga aaaaatcgtg gaacaaaaag atgcaacacc agaagaagct cttgtacaag
     1861 aaatcaatac aggaacttac tgttttgaca acgaagcttt gttcgatgcg ttaagcaaag
     1921 ttggaacaaa caatgctcaa ggtgaatact atctaacaga tatcatcgaa atcttaaaag
     1981 aagaaggcca tacagttgct gcttatcaaa cggatgattt tgaagaatca atgggagtca
     2041 atgatcggat cgcgttagct aaagcaaatg agatcatgag aaagcgaatt aaccagatgc
     2101 atatggtcaa tggcgtaagc tttgtcgatt cagcaacgac atacatcgat gcaggcgtag
     2161 aaatcggtcc agacacattg atcgaagcag gtgtacaaat ccaaggaaat acagtcattg
     2221 gttctgattg tgtcatcggg tcacattcaa aaatcgtaga cagtaggatt gaggatcacg
     2281 tcgtcattga aaactcagtc attgaaagca gccatgtcaa gaaacatgca gatgtgggac
     2341 cttatgcaca tttacgacca aaagctgaaa ttggcgaaaa cgtccatatc ggaaactttg
     2401 tggaagtgaa aaatgctcaa atcggcaaag gaactaaagt cggtcacttg acttatgttg
     2461 gtgatgctac gctgggagaa gaaatcaatg tcggatgtgg tgttgtcttc gtcaactatg
     2521 atggaaagaa caaacatcat acgactgtcg gcgaccacag ctttattggt tctagcacga
     2581 atatcatcgg acctgtcgag gtagctaaaa acagttcgat tgctgccggg tcaacaatta
     2641 cagacaatat tccagaatat gcgctggcaa ttgcgagagc aagacaagtc aataaagaag
     2701 gatatgcaaa aaaactccct tatctaaact aagacttttg caaaatcact tgatttattt
     2761 aaaaaatatg actactattt attaggtaga attctagagt tgtgaaaaag aaagtggagg
     2821 tccccatgtc aaaacattac tttgacccaa gattaaaaat ttttgcgtta aattccaatc
     2881 gcccattagc agagaaaatt gcagcagcag tcggcgtcga gttaggaaaa tcatctgtta
     2941 ctcaatttag tgatggagaa attcaagtaa acattgaaga aagtattcgt ggatcgcatg
     3001 tctatgttat tcaatcaacg agcagcccag tcaatgataa cttgatggag ttgctgatca
     3061 tgattgacgc actgaaacga gcaagtgcaa aaacaatcaa cgtagtcatg ccttattatg
     3121 gctatgcgcg tcaagaccgt aaagcacgtg cacgggaacc aatcacagcg aaattagtag
     3181 caaacatgat tcaaaaagct ggtgcaacaa gaatgctgac tttggacctt catgcagtcc
     3241 aaatccaagg tttctttgat attccagtcg atcatttgat gggtgcgcca cttattgcca
     3301 attatttctt agaaaaagga atcaaaggcg atgatgttgt cgttgtttca ccagaccatg
     3361 gtggtgtcac acgggcacgt aaattagcgg aatttttgaa atctccgatt gcgatcatag
     3421 acaaacgccg tccaaaagca aatgttgcag aagtaatgaa tatcattgga caagtcaaag
     3481 gcaaaacttg tgtactgatc gatgatatga tcgatacagc aggtacgatc acattggctg
     3541 ccaatgcttt gaaagaagct ggtgcaacta gtgtgtatgc ttcatgtaca catccagtat
     3601 tatccggccc tgctttacaa agaatagaag attcagctat cgagcgtcta gttgttacag
     3661 actcgatcta tcttccagaa gatcgtaaaa tcgacaaaat cgatgaagta agtgttggcg
     3721 gactaatggg agatgcaatc aaacgtatcc atgaaaacaa accagttagt ccattgtttg
     3781 aaacaaaaaa taaataataa ataaactcac aaaggggagt ccaattgggc tgagattgaa
     3841 tctatttcta aacccttcgt acctgtatcg gttatgcgag cgtaggaatt gtgaataaac
     3901 agctgtttga ctattttcga aagctgcttc ctcctttgtg acttttttat tcaaaggagg
     3961 aagttttttt atgggaaaaa atagagtttg ggtagaagga acgattgttg cagcgttagc
     4021 gatggtgttg tcgttgattc cgatccagat cggcagcagt ttttctattt cattagggca
     4081 gattccattg actctttttg cgttaagaag agggacaaag gcagggatgc tggcaggttt
     4141 catttggggc atcctacatt ttccgttggg acaagtttat tatttaagtg tcgttcaagt
     4201 gttgattgag tacccgattg cctatacttt tgcaggcgca gcagggctca tggcttcaaa
     4261 tgtccaaaaa ggctatgtag acgataatac gaaacaaatt agaaaaagca tcgtgtttgg
     4321 tgcattgatt ggcgcacttt ccagatattt ttggcatttt attgcaggag tggttttctg
     4381 gggagcgtat gcactatggg ggatgaatcc ttggctcttt tctttcgtaa tgaatggtgc
     4441 cagcgggttg agtacagcta ttgtaacgat catcgtttta ttagggatcc aacaaacgac
     4501 tccttcgatt tttgtgccag ataaacgaca ggagctgtaa ataaaaaaga agcaagaaac
     4561 ttgagacaga atcatgtcct agtttcttgt ttccttttta ttttttgata agaaatagat
     4621 ctacttcaat ggcagataag aaagatcggc atcttctacc aattgccagt gtcgatcggt
     4681 gacagagtag attaaagtag cgatgatttc ctcttctcga taccgggtgc cttttaattt
     4741 ccaaatataa tcttcaatca catagattgg gatacgatat ttaccatcga tcaaaccaac
     4801 ttgatcaaaa gaaaaaatga tcccttcctg catcaaatta cgcatttctt tcaataattc
     4861 cacactgatt ttttcaacag gtgtttcttt tttgaaaaaa gattttttta cattgatctt
     4921 atctgacaag atcgaactta atttactagc aaaaatccta taaaattcat ctaaatagcg
     4981 ataataaatc cgattcagct gatcattaag atcgaagtat acaaaattat tctgtagttt
     5041 ataaaaaaac ggcgaatgaa gatgtgtttt catatgacca aaatataata attctgaaat
     5101 ttctaaaggt gtcaattctt tcagcatcgt taaatcagta aagtcgatcc acttcaattc
     5161 atcgaatcca cgcctgttac gagaagagga aaagaaacgc tgaatatttt ctgtaccacg
     5221 gattactttc agccctgtat gggattcata ttctccagca gaagcagatg gatcaagcaa
     5281 gagcaagttt tgaggaaaat gcacgatact gtttgcaaag tcagtagcag tcagcccttt
     5341 tgttaaaaca gcattacttg tggtatcaat atgtacataa actgactcag tcatcatgtt
     5401 cctcctctta tttcctctta atctctttta ttttacaaca tttgcattaa aaaagcttgt
     5461 ttcttttatt ttaaaattcc attacaaaat cacgttttca ttgaaaacac tctcattttc
     5521 aattaatttt tgtgattttt gtattttttt atttaaagga ggttttatga attgttttct
     5581 tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta ctggtatact
     5641 tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag aaaatgtcgc
     5701 tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt catcaagaaa
     5761 gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa
     5821 aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt aataatctgt
     5881 tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa gcttgcgctc
     5941 gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca gaagaatggt
     6001 tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa aagctggtct
     6061 atcatacagc acttgtttta ggtagaggag gagcgcacgg tgcttatcag attggtgtgt
     6121 ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc tcggtaggtg
     6181 cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa
     6241 agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg
     6301 aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa ggcggggttt
     6361 cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta ctcaaacata
     6421 atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg gaagtagtga
     6481 ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc tctgcttcat
     6541 tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc
     6601 gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca tttgtcgtag
     6661 atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt atccattgga
     6721 aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa aggaaccaat
     6781 taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac tatgggaact
     6841 ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt ttgtcttact
     6901 taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt tggcaaaaac
     6961 tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg atgctagaac
     7021 ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac atgatgattc
     7081 aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca caaattggtc
     7141 aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc
     7201 gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat cgtttgcagc
     7261 atgaattgct taagcaacca gttgacacat tactgatcct atatttatat tatctcaagg
     7321 aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg ctatattgtc
     7381 agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg gtcgcccgcc
     7441 aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag gcctgacatt
     7501 gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat gaggaaggac
     7561 cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca aaagcgtatc
     7621 agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa atacgaagtc
     7681 ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag cagttaagcc
     7741 gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa aaacagatcg
     7801 tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga attcaacaac
     7861 gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc gtgctggacg
     7921 aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg gcacagttag
     7981 ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc
     8041 aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac
     8101 tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg
     8161 aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta gccatcttgt
     8221 tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta caaaaactag
     8281 atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg atccaagaaa
     8341 tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag
     8401 tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat attgaatgac
     8461 gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa gtggaaaaac
     8521 tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga aagatgcctg
     8581 tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt acgtgacgat
     8641 gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga cagaaaaagc
     8701 acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata tgggctttta
     8761 gcaaggatgc tgaacaaaga aatacaataa ataaacagaa ttggatttct tcgcttagct
     8821 tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta
     8881 ttttgaataa gtgatgttca aaaaccattt tctcggtatt aagccaaatt tatgcaaaaa
     8941 tgtgagaaac tattttcaca aaatttttct tcatactcga aactaatcgg cttcttcacg
     9001 acctcttata cacggcgttc aaaagcttgc atctgcgata ataaccccaa ccaaacgaaa
     9061 aacatgagaa gctgtttatg tttggctgtt cttattactt gatgcaggac agctttttta
     9121 cgacctcttg gtacagggta gcttttttac caatttctag ctataattaa agataacctt
     9181 gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta gttgaaagga
     9241 cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct ggtccatccg gtgtaggaaa
     9301 aggcacagta cgaaaagcaa tttttgagag tgaggataat gattttcagt attcgatctc
     9361 aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt tcttccgaag
     9421 caaagaagag tttgaatcaa tgatcgaagc tggagagatg ctggaatacg cagaatatgt
     9481 cgggaattat tatggaactc ctttgtcata tgtcaacaag acattggatg aaggaaaaga
     9541 tgttttcttg gagattgaag tccaaggagc aaaacaagtc aaagaaaaag tgcccgatgg
     9601 tgtattcatc tttttgactc cgccagattt agcggaatta aaatcacgga tcgtaggccg
     9661 aggaacagat gcagatgaag taatcgaaaa acgaatgaaa gttgcaaaag aagaaatcga
     9721 aatgatggct ctttacgact atgctgttgt aaatgatgaa gtgccgctag cagttgaacg
     9781 aatcaaaaac attattgcca gtgaacattt tcgagtagat cgcgtaatcg gtaaatatat
     9841 taaaatgtta aaggagatgt agtcttatga tgttaaaacc ttcaattgac tcactattag
     9901 atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag taaacgcgcg catgaattgg
     9961 atgcaagtgc acagcctact ctagattctt ttgattcagt aaaaagcgtc ggtcaagctt
    10021 tagaagaaat cgatgcagga aatgtagtca atgatccaca tcctgaatta aaacgtgaac
    10081 gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa cacgaattgg
    10141 aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa aatagagcga acatctaaaa
    10201 agaaaagcaa acccagctgt ttccttttca aacattcgcc acttattgtc tggtagaaaa
    10261 ggctgtgtct gaactttttg acacagttct tttttttctt atctgctgtt aattttttgt
    10321 taggatttgg cagaagataa gacaaatcca tcgatctcta gtaaaatagg caaggaactt
    10381 tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca ttaacagctg
    10441 aagtcattgt ggacgtacct actatgcaga ccgatcagcc attcacttat ttggttccct
    10501 ctgaaatgga aacagctttg caagtcggga tgcgagtaga agtccctttt ggtaatggca
    10561 atcgtcatgt ccaaggattt gttatggcta tccaaaaatc agaagaatca gctaatccat
    10621 ctttgaaggc agttattcgt ttgctagacc ttgctccggt ggtcaatgaa gaattacttt
    10681 cattagctga ttatatgaaa aaaattactt acgcattcaa aatcacttgt ctacaaacga
    10741 tgcttccgag tgtgatgaaa gcagaatatg ataaattgat ctatcctttg gcagacacac
    10801 cagaagttca ggtattgttt gatcagcgag aagtgatttc ctggaaggaa gcagaagaag
    10861 aaggcagcct ttctcaattg attcgctggc gccaagagca attagtagat atcaaatatg
    10921 aagtccatac acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg ttaactgaaa
    10981 agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa aaagaacttc
    11041 ttttgtgttt atctgaactg agccaagaag aaccaattgc ttatttcaaa aataaaggaa
    11101 tcagtactgc tgtcttgaat caaggaaaag aaaagggctg gctggagttt atagaatcag
    11161 aacgttatcg cgacccttat aaagaccgag tatttgacca aacgacggca ttggaactga
    11221 atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag caaaaagatc
    11281 aggtcttttt gctggaggga atcactggca gcgggaaaac agaagtatat cttcaggcaa
    11341 tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa atcgcattga
    11401 ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg gtcttacata
    11461 gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg gaagaagcgc
    11521 aagtagtagt aggcgcacga tctgctattt ttgccccgct gaaaaatatc gggctgatca
    11581 ttatcgatga agaacatgaa tcaagttaca aacaagatga gacaccgcgt tatcatgcga
    11641 gagatctagc aatttggcgc agtaaatatc atcattgccc aatagttcta ggaagcgcga
    11701 cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta ttgcagttaa
    11761 accaccgagc caaagcagct gcacagcttc cagctattga gatcgtagat atgcgtgaag
    11821 agtttcagaa tcatcggacc tctacttttt ctgctaattt gcaggaaaaa atacaaaatc
    11881 gattagacaa aaaagaacaa actgtcctac tgctcaatcg tcgaggatat tcgtcttttg
    11941 tgatgtgtag ggactgtgga tttgttcttc catgtcctaa ttgcgatatc tctcttacac
    12001 tgcatatgga tacacgttca atgcgctgcc attattgcgg acatgaagaa cctattccca
    12061 atcgctgtcc taattgtggc ggcaataaaa tccgctacta tggaacaggt actcaaaaag
    12121 tagaagagga actccgagaa ctttatccgc aagctaggat tttgcgtatg gatgtagata
    12181 ccactcgaag aaaaggggca cacgaacaga ttttacaaaa attcggtgca aaagaagcag
    12241 atatattatt agggacacaa atgattgcta aaggtttgga ctttccagaa gtgacattag
    12301 ttggggtgtt gaatgctgat acctccctga atttaccgga ttttcgttct agtgagcata
    12361 cattccaatt gcttacccaa gtttcaggac gagcagggcg agcggaaaaa gcaggcgaag
    12421 tcgtgatcca aacattcaat cctcaacatt acgccataga attggctaaa aaacaaaatt
    12481 atgagcagtt ttatcagcag gaaatgcatg tacggcatcg tggaggctat cctccttatt
    12541 actttactgt gaaaataacc gccagccatc ctgaagaaca agtagcggca aagaaaatct
    12601 ttcagattgc caatcagcta aaagaagtgt taagccctca aagcttgcta ttagggccaa
    12661 cgcccagcat gattttgaga gtgaaaaacc gatattatta tcaactaatc atcaaatata
    12721 aacatgaacc gaatcttcct catgttttgg atgaaatttt gaatggaagc cagaaagaac
    12781 agcgacaagg tctttttgtg gcaattgaca acgaaccatt aaactttatt taagaaaggg
    12841 ccaaattatg cgctatccta tattgatcca ccctaatgat aaattaaaaa gaactgctca
    12901 gccaatcgat gtaatcacgg acgagacaat cgctttatta gacaatttat atgagacaat
    12961 gattgcgaat gacggaatag ggatcgctgc tcctcaagta ggacaaaaca agagaattgc
    13021 tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt aacccagaaa tcatcgaagc
    13081 aaaaggtgaa agccttgacg tagaaggttg tctcagtatt ccgcatgtct atggaacggt
    13141 caagcgagca gatgaagtga ccgtgcgtta ttatgatcga gacggagaag agatagaagt
    13201 gacggctttt ggctatttag cccgtgcctt ccagcatgaa atcgatcact tagacggtat
    13261 cttgttcatt gagaaaatga tacagcagat cccggaagaa gaacttgaag aatatatgga
    13321 ggaacatctt gatgactaag ctagtattta tgggcacacc tgctttttct gtgccgattc
    13381 ttgaagggct gatagaatca ggttacgaga tccaggcagt ggtaacacag cccgaccgtc
    13441 cagttgggcg gaaacgagta attacgccga caccggtaaa aaaagcggct ctgaagcacg
    13501 gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc agaaatggaa gaaatcattg
    13561 aattggtgcc tgatgtgatc gtcactgcgg catttggcca attcttgcct gaaaagttat
    13621 tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc tttacttcct aaatatcggg
    13681 gaggtgcgcc tgttcattat gccatcatga atggggagaa agaaactggt gtaacgatca
    13741 tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc ataccaatca
    13801 ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag ccttttagga agaaagctat
    13861 tgctagaaac attgcccaat attttggatg gacaaaaacc agtacctcaa gacgagtcag
    13921 aagcaacttt ttcgccaaac atcacacggg aacaagaagc aatcgattgg cataaaactg
    13981 cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca ttcacgacct
    14041 atgagggcat acgctggaaa ttattatcgg ttgaagtatt ggcagaaact acagacaaag
    14101 cacctggtac gatcatccgg aaagacaaaa aaaatctatg gattgcttgc ggtaaacaga
    14161 ccgtcttagc catcaatgaa ttacagccag ctggtaaagg aaaacaggcc gtacatgatt
    14221 tccttaacgg aagcggtcag caagttcaga ttggtcagca ggtgaaataa tgaaaaaaat
    14281 ccctaaaaaa atgaaaaaat cagtacgata tatggcatta ctgacattag agcgagtgaa
    14341 caagggtggc gcctactcga atcttttgtt gaatgagatg atcaaacaaa gcgagctaaa
    14401 tcaaaaagat gtgggtttat ttacagaatt gatctatggt acgattagcc atcagcgact
    14461 attggaattt tacgttgcac cattgatcgc taaagcaaaa aaagtggatg actgggtcaa
    14521 gatgcttctt tatctttctg tataccaatt agaattttta gataaagtcc ctgcacatgc
    14581 tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga cagggaaatt
    14641 tgtaaatggt gtcttgagga attatcagcg tcaaggagca ccagatctgc aaggcattgc
    14701 tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct ctatggatga cgaaacgact
    14761 ggtggatcaa atcggatacg aagaaactga aaagctcggt ttatcgctat atcagcctag
    14821 ccatgctagt gcccgagtag atacacgccg actttccaga gatcaggcca tcactgtttt
    14881 gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt ccatacggta tagtagcgaa
    14941 aaaagggcat ttagcttcta gctcattgtt ccacgatggc gtcatgacga tccaagatga
    15001 aagttcgatg cttgtagcgc cgtctatgca aatcgagcct gaacatcatg tcttagatgc
    15061 ctgtgcagca cccggaggta agacgacgca tattgcttct tttttagatc cgtcatgcgg
    15121 tggagaagtg gtcgctttag atgtacacga acacaaagtc aaactcattg aagataatgc
    15181 agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta gagaagtcag
    15241 taaacagttc gaagcagagc agtttgatcg gattttagta gacgcgcctt gttcaggtct
    15301 tgggctgctt cggcgaaaac cagatattcg ttataaaaaa cagccggatg aattagcgaa
    15361 tttaccaaaa atccaattgg aaattttaga aagctgcgca ccaactttaa agtcttccgg
    15421 tattctgact tatagcactt gtacgatctt aaaagaagaa aaccaagacg ttgtttctgc
    15481 tttcttggaa agacaccctg attttgagag gattgatgtt ttgaccaatc ctatgctaca
    15541 atcatcaatc gaagagaaaa tgttgacttt gtatccgcat cagttttaca cagatggatt
    15601 tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg aatatcaatc
    15661 agatgtagga agacgacgta acacaaatca agactatgcc agtgtattta ccagccgttc
    15721 aggtctcaag cttgctgtat tagcagacgg gatgggtggt catcgtgccg gagacattgc
    15781 cagtcagatg gcggtaacga atctaggcga agcttgggaa aaacaagaag ctgttgacga
    15841 tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa gaaaatatgc tgatttatca
    15901 gcgtggacaa gaacagccgg aatataacgg tatgggaaca acaatcgtag cggccgctct
    15961 gtcagatgac tggttcacga ttgccaatgt tggggatagc agagcttatt tgattcgtga
    16021 agataaaatc atgcaattga cggaggatca ttctttagtc aatgaactag tcaaatcagg
    16081 agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa aatattttaa cacgttctgt
    16141 cggcatgcct ggaacagtcg aagtagatgt ttctactcat ctttggcaac taaaagatcg
    16201 actgctgtta tgttcagatg gcttgacgaa catgctgtct gaagaagaaa taggaacgat
    16261 catcaatcaa gaaggcccat tgtctcataa agtaactgag ctgattgatc gtgccaatga
    16321 agcaggcgga gcggataata tcaccgttct tcttatcgaa tataaggagg acagtgtatg
    16381 atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat cggtagcggt
    16441 ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt agcagttaaa
    16501 gtcttacgat ttgactttca aaatgaccag actgcgattc gacgatttca gagagaagct
    16561 ttagcagcaa cagaactcgt tcatccaaac attgttagtg tttacgatgt tggtgaagaa
    16621 gacaacatgc agtatctggt gatggaatac gtcaaaggaa tggacttgaa acgatatatc
    16681 caaacacatt atcctgttcc ttatgaaaca gcagtaaata ttatgcagca gatcctttct
    16741 gctatttctt tagctcataa ccatcaaatc atccaccgag atttgaaacc ccaaaacgta
    16801 ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg ggattgctat cgctttatct
    16861 gaaacatcga tcacgcagac gaatacgatg ctaggatctg ttcattatct gtcccctgag
    16921 caggccagag gaagtatggc aacgaagcag tcagatattt atgctttagg gatcatcctt
    16981 tatgagatgt tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac aattgcttta
    17041 aaacattttc aagatgatct tccatctata aaagcacttg atccaaatgt gccacaagca
    17101 ctggaaaacg tgattttacg ggcaacggct aaagaacctg cagaccgtta taagtcagcc
    17161 gaggaaatgt ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa cgaagaaaaa
    17221 tggcagcctc atgtgatgga taatgaaaca aaagttatca caccccttac ggaagatgca
    17281 ccaatgcctg attcattcaa atctatgcct ctgccaaaag acaagccaga aaacgaaacc
    17341 ttaccagagg aggaagaagt tctgcctgat cctaaaaaga aaggacagaa aaaatggtgg
    17401 ctgatcctat tagctattct tgctgtttta ggaatcggaa cagctgtatt ctttgcttct
    17461 ggcgggcgag gagaagttgt tgttcctgat gtttctgatc tttctgaagc ttctgctaga
    17521 gaaacactcg caaaagcgaa attagaagta gcaacaaaaa cagaagaaat tgcagatgac
    17581 aaaatcgaag aaggtaatgt agtgaagaca gacccggcag cgggaacgac agtcaagcaa
    17641 aaacgcgaag tcactctgta tattagtagc ggcacgaaaa aaatcaaatt agatgattac
    17701 agtggtaaaa gttatgaaga tgccgaagca agtttgaaag aattagggtt ttctgctgac
    17761 ttgattcaaa aagaagagga attcagttca gaggtagaaa gcggaatgat catttcgcaa
    17821 tctccatcag aagatacaga agtcgatcct aaaacagaca cgattacttt cacagtcagc
    17881 aaaggaccag aaacagtgac tttgagtagt catgtaggag aaaactacga aactgttcgt
    17941 aattggcttg ttacacaagg attcaatatc aataatatta gtgctactta tgactatagc
    18001 gacactgtac catcaggttc gatcatcagt caggatccag cctcgggtga agttgtagca
    18061 gaagaaactt acatcaattt tgtcgtaagc caaggaacag agcctaaatt gaatgatatc
    18121 tccggctata caaaaagcga agcacaaagt tatttagcta gtgtcggtgc agaatatatc
    18181 gggcatgaga cttccgaata ttcaaacacg gtggataaag ataaagtgat tcgtacagta
    18241 ccaggggcag gaacgacaat taccaaagga atggttgtga acgtaattta ctctaaagga
    18301 ccagatccag cgtcttctag ttctgaaaaa gactcttcag acagttctag cagctcaagt
    18361 agctccagta gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac aagttcatca
    18421 gctagcacct cagaaagttc aaaataagtg ctgaataaaa tatatcagaa gggaatgcga
    18481 cattgatttg ttgcattctc ttttttgtgt ttctatctct tttgatcaat tttcaagcca
    18541 tatactttga gaagtttgca tggaataaca cctactttcc tcttcgtatg gtaaaataaa
    18601 caaaaacgga ggtggcactt atcaaaggac aaatcagaaa agcaatcagc ggtttttact
    18661 atgtatacgc aaacggagag acctatcaaa caagaggaag aggaaatttc cggaatcgga
    18721 aaatcacgcc gcttgtcgga gatcaggtga tctttgagag tgaaaatcaa acagacggct
    18781 atcttctgga aatcttgccg agaaaaaatc aattagttcg cccaccagtc acaaatgttg
    18841 atcttggaat cattgtcaca agtatcgtag agcctgcatt ttcttataat ctacttgacc
    18901 gctttctagt taccttggaa tacgagtcga tcgaaccgat tatttttttg acaaagaccg
    18961 acttgtcaga cgacccagaa aaaatcgaag agatcaagca aacctatact aaaataggct
    19021 atccggtgat tgtacccaat catacgggtg atacgacaga actgatccgt tacttcccag
    19081 aacggctatc ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta ctgaatcaaa
    19141 tatcaccgga actgaattta gcaacaggag agatttctga tgcacttgga agaggacggc
    19201 atacaacacg tcatgtggaa ctgattccta tctatggcgg gttagtagca gatacaccag
    19261 gatttagctc gattgacttt ttgacaattg aagcaggaga actgccgaga caatttcctg
    19321 aatttgtaga agcttctgtc cattgcaaat ttagagagtg catgcatgct aaagaacctg
    19381 gctgcgaagt aaaacataga gtagaaacag gtggtatcgc acagacacgt tacgacaatt
    19441 atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa aaagaaggta
    19501 aacgaaaata ggaggaaaca agaatgaaga tagcaccatc cattttgagt gcagattttg
    19561 ccaatttaca aagagacatt gagttagtgg aaaaaggagg cgcagattac atccacgtag
    19621 atgtaatgga tggccgattt gtcccaaata tcacattagg gccaaatatt gttcaggcaa
    19681 tccgtcctgt tactaaatta ccgttagacg tccatctgat gatcgttgat ccagaaaact
    19741 acattccagc atttgcaaaa gctggagcag atatcattac tgtccacgtc gaagcaactc
    19801 ctcatattca tcgggcactt caaatgacaa aaagcttagg agttaaagct ggagtagtta
    19861 tcaatccagg aacaccagtc tccatgatca aacatgtttt gcctttggct gatcaagtac
    19921 tagtcatgac tgtcaatcct gggttcggcg gacaaagctt tattgaagaa acagtagaaa
    19981 aaattacgga actcgctgat ctacgtagag agaacaattg gacttatgca atcgaagtag
    20041 atggcggaat cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg gatgtcttcg
    20101 ttgcaggatc atatatctat ggagccgaaa atccagtcgg acaaatcgaa cgattaaaag
    20161 aggcattaga ctaaatgaaa attttattag ctgctggagg taatccgcaa aactggccgc
    20221 agtctctgct gtccaaatac gatgcctatg tcgggataga ccgtggtgcg ctgtacctgc
    20281 ttcaagcagg ctacccagta gatctggcag tgggtgattt tgattcattg tcgaacgaag
    20341 agcagaagga agtatttgca aaagccaaag agtatacccg ttcccctgca gaaaaagacg
    20401 atacagatac acagttaggt atcttaaaaa catttgaacg ttacccgcaa gcggaagtca
    20461 ctttgattgg agctacgggc ggaagattgg atcatctttt agccaatctc tggttaggta
    20521 tggaacctcg cttcaaaccc tttgcccatc agcttattat cagagattgt caaaattatt
    20581 tgtcttatta tttgcctgga gagcatatag taagacagat accagatatg aagtatttag
    20641 cttattgctg cttgactccc gtctcgaatc ttacattaaa aggcagcaag tatgtattgg
    20701 aaaataaaga agtagaatat ccgacatctt atgcaagtaa cgaatttctt tcagataccg
    20761 cagaattttc tttttcagat ggtatgattg cagttattca aagcaaagat tgatccataa
    20821 acaaataaaa aaatttgtct ttcaggctaa aaatgacaga atctattatt tgctaaaaaa
    20881 aagcttaaga ggagcaactt accttttgtt tcacggcatt tagtgataaa attgaacaca
    20941 agagtctaat taattcgagg tgtctttgtg aagaagttca atcctaataa aaatattatt
    21001 attaccttgg ttattgttat catcgtggtg acgatactaa gtgtgaccgc tgcgcagcga
    21061 gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta atgacagtgt tgcaatggtt
    21121 gataaaacaa tctctgcccc agtacggtgg gtacgaaatg gagtagattc tgtccataat
    21181 cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag ctatgatgac
    21241 gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa agaacttgag
    21301 ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa atgtcattac tcgttcgcct
    21361 gacagttggc aggacatgct gatcgtagac aagggttcta aagacggtat cgaagcaaat
    21421 atggcagtca tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa tacggcttcg
    21481 tctaaaatcg aattactgtc atcctctaat gaaagctcca atcattttcc agtacgggta
    21541 tcttcggcta atggcgaagc gtttggtttg cttaaaaact atgatgaaaa gctccatgcc
    21601 ttagtggtga cccaattaac tggtgatacg gatatcaaag aaggggatgt tgtccagaca
    21661 tccggtcttg gagggaattc tccagctaac ttgccgatcg gtacggttat taaaacgaaa
    21721 ccagatagtt atgggctgga tcgggaagtt tatgtgaaac cttatgcaga aatgtatgac
    21781 gtgtcagttg tgacgattgt ccaacggcta gcggaggatt agtccatgtt gaagaaagaa
    21841 acaatgaagt actatctgcc aatcgttttg ttctttttga tgttgataga tggtcattta
    21901 acaagaatgc taggggagtg gtcgaaaggc acctatatgt caaatgccca ctttctgata
    21961 ttggcattat tatgttgcag tatggcgttt gaaaaacgtt atttactgat taccacgatt
    22021 gttctcgggg ctatctatga cgcttactat attggcgtta tcggtatcta tgcagtagct
    22081 ctccctttaa ttgtatggtt gatgtatgta atgaaagacg ttatccatgt caacatcttt
    22141 actgaatttt tcagtatgat catctttgtc acgggttatg aattgtttac gatggtggtc
    22201 cagttgattt ttaaattagc agtagtaaat aacacgtatt ttattacaag gtttttagga
    22261 cctacactgc tgttgaacat gattatattt gtattattca tttttccctt taagaaatta
    22321 ttcagtgatg aataatttct ttttttattt ttaggtcgaa agcgaaaaag aatcgttgtt
    22381 atcttaagaa atgctagact taacgcggct ttttcatgat cattttctga aacgaacagt
    22441 tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac ataactatta tatcgttgac
    22501 acactgctat ggtacaattt ttatatcgtt aagaaagact taaatatatt tagatattta
    22561 ttaaattaag ccagtcggag gaatgaagag tgaaaaagag tttaatatca gcagtaatgg
    22621 tatgttcaat gaccttgact gccgtagcat caccgatcgc tgccgcggcg gacgattttg
    22681 attctcagat acaacagcaa gatcaaaaga ttgcagactt gaaaaatcaa caagcagatg
    22741 cacaatcaca aatcgatgcc ttagaaagcc aagtttctga aatcaacaca caagcacaag
    22801 acctattagc aaaacaagat acattgcgtc aagaatctgc acaattagta aaagacattg
    22861 cagatttaca agaacgtatt gaaaaacgtg aagatacaat ccaaaaacaa gcacgcgaag
    22921 cacaagtaag caacacaagc tcaaactaca tcgatgctgt attgaatgct gattcattag
    22981 cagatgctat tggacgcgtt caagcaatga caacgatggt aaaagcaaac aacgacttga
    23041 tggaacaaca aaaacaagat aaaaaagctg tagaagacaa aaaagctgaa aacgatgcta
    23101 aactaaaaga attagctgaa aaccaagctg ctttggaatc tcaaaaaggc gatcttttat
    23161 ctaaacaagc tgatttgaac gtactgaaaa cttcattagc tgctgaacaa gcaactgctg
    23221 aagacaagaa agctgacttg aaccgtcaaa aagcagaagc tgaagcagag caagctcgta
    23281 ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca agctgcacaa gaaaaagctg
    23341 aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc tactcaagct tcatctactg
    23401 cacaaagctc agcaacagaa gaatcatcag ctacacaatc ttcaatgact gaagaatcat
    23461 catcagctac acaatcttca gcaacagaag aatcaacaac acctgaatct tcaacagaag
    23521 aatcaacagc gcctgaatct tcagcaacag aagaatcaac aacagcgcct gaatcttcag
    23581 caacagaaga atcaacaaca gtgcctgaat cttcagcaac agaagaatca acaacagtgc
    23641 ctgaatcttc aacaacagaa gaatcaacaa cacctgcgcc tacaacacca tcaacagatc
    23701 aaagtgttga tacaggaaac ggcacaggaa gtagtactcc ggctccaacg ccaacaccaa
    23761 cacctgaaca accaaaacct gtaacacctg ctccagctcc ttctggaagt gtgaatggtg
    23821 cagcaatcgt agcagaagct tacaaatata ttggtactcc atatgtttgg ggcggtaaag
    23881 atccaagtgg ctttgactgc tcagggttca cacgctatgt ttacatgcaa gtaactggtc
    23941 gtgacatcgg tggttggaca gttcctcaag aatcagctgg aacaaaaatt tcagtttctc
    24001 aagcaaaagc tggagacttg ttattctggg ggtcacaagg cggaacttac cacgtagcga
    24061 ttgctttagg cggaggacaa tatatccatg ctcctcaacc aggcgaaagc gtaaaagttg
    24121 gttcagttca atggtttgcc cctgactttg ctgtcagcat gtaagaatga
//
LOCUS       DAYYAJ010000033        24098 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000033 DAYYAJ010000000
VERSION     DAYYAJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.033"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(268..1455)
                     /locus_tag="ACHATZ_001115"
     CDS             complement(268..1455)
                     /locus_tag="ACHATZ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8403660.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(1562..1774)
                     /locus_tag="ACHATZ_001116"
     CDS             complement(1562..1774)
                     /locus_tag="ACHATZ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HFM8403661.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(1865..2317)
                     /locus_tag="ACHATZ_001117"
     CDS             complement(1865..2317)
                     /locus_tag="ACHATZ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8403662.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2330..2740)
                     /locus_tag="ACHATZ_001118"
     CDS             complement(2330..2740)
                     /locus_tag="ACHATZ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8403663.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            3015..3227
                     /locus_tag="ACHATZ_001119"
     CDS             3015..3227
                     /locus_tag="ACHATZ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8403664.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3230..3442
                     /locus_tag="ACHATZ_001120"
     CDS             3230..3442
                     /locus_tag="ACHATZ_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403665.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3582..3737
                     /locus_tag="ACHATZ_001121"
     CDS             3582..3737
                     /locus_tag="ACHATZ_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403666.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3734..3958)
                     /locus_tag="ACHATZ_001122"
     CDS             complement(3734..3958)
                     /locus_tag="ACHATZ_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8403667.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            4036..4224
                     /locus_tag="ACHATZ_001123"
     CDS             4036..4224
                     /locus_tag="ACHATZ_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403668.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4363..4491
                     /locus_tag="ACHATZ_001124"
     CDS             4363..4491
                     /locus_tag="ACHATZ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403669.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            4550..4885
                     /locus_tag="ACHATZ_001125"
     CDS             4550..4885
                     /locus_tag="ACHATZ_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403670.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5118..6059
                     /locus_tag="ACHATZ_001126"
     CDS             5118..6059
                     /locus_tag="ACHATZ_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8403671.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            6061..6951
                     /locus_tag="ACHATZ_001127"
     CDS             6061..6951
                     /locus_tag="ACHATZ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8403672.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6994..7794
                     /locus_tag="ACHATZ_001128"
     CDS             6994..7794
                     /locus_tag="ACHATZ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8403673.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7809..8660
                     /locus_tag="ACHATZ_001129"
     CDS             7809..8660
                     /locus_tag="ACHATZ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8403674.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8861..9322
                     /locus_tag="ACHATZ_001130"
     CDS             8861..9322
                     /locus_tag="ACHATZ_001130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8403675.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9348..9800
                     /locus_tag="ACHATZ_001131"
     CDS             9348..9800
                     /locus_tag="ACHATZ_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403676.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9906..10100
                     /locus_tag="ACHATZ_001132"
     CDS             9906..10100
                     /locus_tag="ACHATZ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403677.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            10131..10517
                     /locus_tag="ACHATZ_001133"
     CDS             10131..10517
                     /locus_tag="ACHATZ_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403678.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            10514..10717
                     /locus_tag="ACHATZ_001134"
     CDS             10514..10717
                     /locus_tag="ACHATZ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8403679.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            10714..11010
                     /locus_tag="ACHATZ_001135"
     CDS             10714..11010
                     /locus_tag="ACHATZ_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403680.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            11086..11502
                     /locus_tag="ACHATZ_001136"
     CDS             11086..11502
                     /locus_tag="ACHATZ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8403681.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            11763..11900
                     /locus_tag="ACHATZ_001137"
     CDS             11763..11900
                     /locus_tag="ACHATZ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403682.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            12310..12540
                     /locus_tag="ACHATZ_001138"
     CDS             12310..12540
                     /locus_tag="ACHATZ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403683.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            12699..13532
                     /gene="terS"
                     /locus_tag="ACHATZ_001139"
     CDS             12699..13532
                     /gene="terS"
                     /locus_tag="ACHATZ_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8403684.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13525..14940
                     /locus_tag="ACHATZ_001140"
     CDS             13525..14940
                     /locus_tag="ACHATZ_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8403685.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14952..16481
                     /locus_tag="ACHATZ_001141"
     CDS             14952..16481
                     /locus_tag="ACHATZ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8403686.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16399..17451
                     /locus_tag="ACHATZ_001142"
     CDS             16399..17451
                     /locus_tag="ACHATZ_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8403687.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17453..17770
                     /locus_tag="ACHATZ_001143"
     CDS             17453..17770
                     /locus_tag="ACHATZ_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403688.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17897..18520
                     /locus_tag="ACHATZ_001144"
     CDS             17897..18520
                     /locus_tag="ACHATZ_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8403689.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18535..19425
                     /locus_tag="ACHATZ_001145"
     CDS             18535..19425
                     /locus_tag="ACHATZ_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8403690.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19448..19663
                     /locus_tag="ACHATZ_001146"
     CDS             19448..19663
                     /locus_tag="ACHATZ_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403691.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19675..20016
                     /locus_tag="ACHATZ_001147"
     CDS             19675..20016
                     /locus_tag="ACHATZ_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403692.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            20016..20387
                     /locus_tag="ACHATZ_001148"
     CDS             20016..20387
                     /locus_tag="ACHATZ_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403693.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20380..20775
                     /locus_tag="ACHATZ_001149"
     CDS             20380..20775
                     /locus_tag="ACHATZ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8403694.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20777..21154
                     /locus_tag="ACHATZ_001150"
     CDS             20777..21154
                     /locus_tag="ACHATZ_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8403695.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            21155..21763
                     /locus_tag="ACHATZ_001151"
     CDS             21155..21763
                     /locus_tag="ACHATZ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8403696.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21763..22104
                     /locus_tag="ACHATZ_001152"
     CDS             21763..22104
                     /locus_tag="ACHATZ_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403697.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22346..24098
                     /locus_tag="ACHATZ_001153"
                     /pseudo
     CDS             22346..24098
                     /locus_tag="ACHATZ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8598 a         3620 c         4905 g         6975 t
ORIGIN      
        1 aacgataagt gaagaataga cattttttaa tgtttcatca tacttttgat ttatttcatc
       61 atttaaatct tctttctctt tttttaataa attaataagt caagtccaca atcttgtgta
      121 aaagttcaac ctcatcaaaa gcacaatgtt ttgcgttgtg aacctaactg ccatgccttt
      181 ctttctgagg agagaggtca ggagcagtag cgcagcgact cttgatgtct ctcctcagaa
      241 agaagagact cgcacaggtt catggtgcta aatgttattc gaattaaatt ctaaatattt
      301 tcgtggagaa gccagccact cagtgtcttt ttccatcaaa agtgttccaa ttatccgagt
      361 gatggaattg atatttggaa aaatccgaat cactcgttct ctccggcgaa tctcttcgtt
      421 caaccgttcc aacacattgg ttgtacgaat ccttcggcgg atattttcag gaaaagacaa
      481 aatttgaata gcatcttcaa agccgttttc taaaatgtcg caagcagcac ttaattttga
      541 atcacaacca tatttttcca agaagtgctc ttttctttca cgtgtcaatt caagttcaga
      601 ggctttaaag atactcttca actcgtcttt tacatcagaa gaaactttct ttggcagctt
      661 atcaaaaata tttctcaaaa agtgggcttg gcatctttgc cagctcgcac ctaaaaagtt
      721 cttgcgaaca gccttaacta accctttgtg tgcatcagaa atgaccagtt ttacaccgaa
      781 caaacctcgt tgaacgaacg attgaaaaac ggtatcccaa ttatcttccg attcgccgtc
      841 ttgaacatca aaaccaagaa tcgtacgtcg tccatcagaa tcaatgccga taccaatata
      901 tacacctttt gagacaatac gatgattttc ccttactttc atatacaggg catcacacat
      961 gaaaaatgga tattccgagc cttctaggct ccggtttctc cactcaaaaa caaatggatc
     1021 caactgtttc atcagatttg aaacgaatga tttagaaaca gtcgcaccat ccgttagcag
     1081 ttcaactgtt ttagttactt tgcgagtgga gacaccagaa atgaccattt caatcatggc
     1141 agcgatcaaa gctttttcgc ttcgctgata tctttcaaag atagtaggac taaacttacc
     1201 gtctctggtt cgtggaacat gaagttctaa cgtaccaatt cgagttgtaa acgaacgatc
     1261 gtagtaacca tttctttgtg attgccgttc ttctgaacgt tcataggcat ttgcctgaat
     1321 atattgattt cgttgttctt ccatcagctg attaaacatg atagtcataa gtgtttttgc
     1381 ggtatcattc gcaccgcttt cgtttataat tgcttgaatt tcttcgcttt caaaagtaaa
     1441 atgtacttga gtcattgata tcagtcctat tcgagtatgt tttctgcaaa aaacattgta
     1501 ctcctaactg agatcaatgg cttttttgat gcttcacttt tacacaatta tatggacgta
     1561 atcaattaat atcatttttt aacatagtaa tttgtttatt taaatctgat agtgtttctt
     1621 ccttacttaa tataaatctt tctctatcat gtaatttatt tagtttttca tcattttttt
     1681 gttctaattc ataagcttct ttttttattg tgttagcttt cgacagttta gtgattccta
     1741 taattaataa aataagtcca atcaataata acattcaaaa ctcctcattt ctttgatata
     1801 attattgtag agtttactca gaaatgagga aagagtccat gtgaaagcat gggctttttt
     1861 atttttaatg ttgtaatact aaatgttcaa gattatgagg aagccctaaa tattcaataa
     1921 tttggaactg actcatatca gataaatctt gttcatctat tgttctagtt aacatttcaa
     1981 ttgcaaacat attagcttca gcttctatac cataaatcat acaactaagg ttgtttgctc
     2041 taaaagctgc tgtgcaaata cctttatgga gtctaacgtg ggctatttca tgccataata
     2101 caaatctttt catagaaggt gataggtttt gattaattaa tatcacataa catcttttat
     2161 tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt ttcaattaaa tctatatcag
     2221 aatatttgat tattttatat ggattaatcg catcagatcc aaactctaca aataattttt
     2281 ctatttcttc taatatccag tatttgttta aatgcatacc aaagcccacc tattgacgat
     2341 attttttagg agtgaatttt ttctttgctt taactttggt taaggcaagt gcaagttgaa
     2401 tggcatcgta taatttttcc ttatcttcat cgtctaaagg ttcaccataa taatttgttt
     2461 cagcatctga attcaagcct tcaagtaatt tttcagcttg taaagcaata tcttgtttat
     2521 ctttttctgt taaatcataa taatggcgtt tatcagtacg tcctaaaaga taatcagttg
     2581 atacattaaa gtaatctgca actttttgta ttttatctcc agatggagta cttttattcc
     2641 atctagacat gcttccattt ccgaaattta actttctttc aagttctgcc aacgtcattg
     2701 aacgttcttt taataagatt tttatacggt ctaatagcat aggatatgca ttcctttcaa
     2761 aataccgaca aaaaaagtat ttttctgcaa attatctatt tacaaataga gaatttgcag
     2821 ttatactagt tttgtaagca agatatttac ggcatacaaa gactaaaagc aataaactaa
     2881 tagtctcccc agacatcagt aagctcgctt tttgtgcgct gtattttgta tgcttacata
     2941 atagcatatt tgcagttttt gtcaaactta ttagacaaaa atattagaaa atattcgata
     3001 agggggtatg aagcatgact ttaaaacagc aagtattaat tgctttagta aagaaaggtt
     3061 ggagtcaaag agagttagct cgacgaatgg gtatatcaat tacttattta cgagatatct
     3121 tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat cgaagaactt ttggatatca
     3181 agttagaagt agattctaat gatcagccat cagaacagga ggcgtaaaaa tggtacaacg
     3241 tttgagcaaa gcggagttta aagagaaata taagtattct gaaagtactt atcaaagaag
     3301 aatgaaggaa tttaaaaaat cacgttttag tgaggggtat aacgcagtta cttccaaaga
     3361 aataacaatt gatgtcgatt tatatgaaca gtttagaaca tggcgttcta aaaatcgatt
     3421 tagagttaga gctgtttgtt aatagctaaa aaatctaaat tcaccacttc tgaaaagagg
     3481 agttgctgct ctcctttcaa gtttgccaaa gtccacgttt tttcattttg gcaactcctc
     3541 ttcttggagg tggtgacggc gagagacgaa ggagggatga catgaaggaa gaatcaaaaa
     3601 tagggatttt gaatgagaac aattacacaa gattaattat tgaaacagaa gaagggaaaa
     3661 aagtagcaga agttacattg accgatgcaa ctcctgctga cggttatcgt attcgattaa
     3721 ctcctaaata tgattaacct tttggtgggt gtggatcgtt tccgtgacta tcacggcttt
     3781 gaatttttcc atctctgcca tgaataaata gctcagatcc ttgattttta gaaatctctc
     3841 tagcgatttt tactgcttct gattttgtat tagtatgtgc agtcgcttta gaatttccag
     3901 cacctttgac attccaacca ccattggaat taggtacaac atgttgattt tttcccatag
     3961 ttttcacctc catttctata gaaatatttt atcaaataga aatggagaaa ttcaataaac
     4021 gaaaggagaa gtgatatggc atataccttg caacaagaac atcaaattct cggtttgatt
     4081 aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc ttagaaaagc cgatgagcta
     4141 tcagataggc aagctgaact aattgcttct gaacttgagg atttgagaat gctagaaata
     4201 aaaaataggg agattagatt atgaagaaga cagacacact ttttatagga ttcattttgg
     4261 ggttattagt gattgtagcg caccaaagta ttatcggggg aagcttgttc gcagcattga
     4321 tggttttaat caatctgctt gattcaaaag aaaggagcaa ctatggcacg agaagaagcg
     4381 ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa atagccgccc tattatttta
     4441 agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg actctgccag
     4501 ccagcaaaga gtcacataca aatttataaa actaggagaa ttatatcaca tgaataagaa
     4561 aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg tttctattat
     4621 ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga caacagaaat
     4681 tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat tgtcagcaca
     4741 tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag aagaaaatca
     4801 accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg tgatgactcg
     4861 tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact cattatttta
     4921 tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt attcgcaatt
     4981 accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga acgagttagc
     5041 aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg aagcaccaat
     5101 tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa gaatggcttg
     5161 aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac aattttagga ttgaataaat
     5221 ggaaatctcc ttatcaacta tggctagaaa aaacaggaca agtagtactt gaagaatcag
     5281 gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt ggttgctaaa gagtttcagg
     5341 aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca ttacatccat
     5401 ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg gaatgcaaaa
     5461 cagctaatca atttctcggt aaagaatggg aaggcgaaga ggtaccgctt agctatctct
     5521 gtcaagttca acattacatg aacgttctaa acaaagacta ttgttacatc gctgtcttaa
     5581 tcggtggtca aaaatttatc tggaagcgga ttgaacgaga tcaagagctg atcgatacaa
     5641 tcactgaaca attagtagag ttttgggaaa caaacgttct tggaggtatc gagccaatta
     5701 ttgatggtag tcaagcgaca gctgatttct tgaaagagaa gtatgcagat gttgaagacg
     5761 ttcaaacgac gttaccaatt catttcgatg aactagttga acagaaaaac gaactcaaac
     5821 ggactaagaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt atcagcgagc
     5881 taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt tggaaatttg
     5941 tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat gtcgcaaatg
     6001 atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaagggag atcaaataat
     6061 atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa agccacagaa acaagttgca
     6121 ccaggtcaat taggacttaa agctttgatg aatacaccaa caatgagaaa gaagttcgaa
     6181 gaggtgcttc atgacaatgc taatgctttt atgtcgaatg ttatgactct tgtatctaat
     6241 gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt aactgctgca
     6301 acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc attcaatagt
     6361 aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg ctataaagga
     6421 tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat cgaagtttac
     6481 gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga tccaaatggt
     6541 agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa tggattcaaa
     6601 aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa caataaagat
     6661 cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat ggcacgaaaa
     6721 actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat gcaagaagcc
     6781 accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat tgctgaaaca
     6841 gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga agaaaatgaa
     6901 gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata atgagggagc
     6961 tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt caaattacat
     7021 cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt atggaacttg
     7081 tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa agagatacaa
     7141 gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat gaacgaaggc
     7201 gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa acaatttgaa
     7261 aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc aataaaaaac
     7321 tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac aagtgaacaa
     7381 caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa taaaacatca
     7441 tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta cattctcgcg
     7501 gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa tctgcaaagt
     7561 tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa ccaggtacgg
     7621 ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt atctgctaaa
     7681 aaattgcgag aaaaatacga cacaatggca gcgcaagcca atcgtgatta taaaacaaaa
     7741 caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat ttgagaggag
     7801 gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa gtgcttgtcc
     7861 aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg aagaaccctg
     7921 atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca ttgcgagttc
     7981 aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga gcaatcaatt
     8041 ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca ttgcagaatt
     8101 atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc tttgttaatt
     8161 cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga acaggtaaaa
     8221 gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac gataaaaaaa
     8281 tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac aacaatcctg
     8341 aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg gatttagtca
     8401 tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga gcgtttggca
     8461 taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt atcacaacga
     8521 acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca agaatgtttg
     8581 tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc ataaaaccag
     8641 tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc gtcagataat
     8701 gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga gtcgaatggt
     8761 tcggaagata agtaagctaa ttatttatcg ggatacagaa agatttctca ctgttttagg
     8821 taacgtctac gagaatccag agttattgga ggtagagtaa ttgccaaaaa tattagatgc
     8881 atgttgcggt agtagattgt tctggtttga taagaacaat tctgacgtaa catttatgga
     8941 ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg atcaatgttg atccagatgt
     9001 ggtcgcagac tttcgcgata tgccttttga agatgatgtg ttcgatatgg ttgtatttga
     9061 cccgccacac ttaagacatg tcggcgaaaa ctcatggctt gccaaaaaat atggaaaatt
     9121 agacgaactt tggccagaag acatcaggca aggttttgca gagtgtatgc gagttttacg
     9181 accttcagga acattaattt ttaagtggaa cgaggaacag ataccacttt ctgatgtgtt
     9241 agaggctatt ggagaacagc cattgtttgg taataaacga agcaaaacac attggcttgt
     9301 gtttatgaaa aaattcagct aacggaggaa acaaaggagg aaaagtaatg aagattgtat
     9361 taaataaatg ttttggtggc tttgggttga gccatgaagc aaaaatggaa atttttaaaa
     9421 gaaaaaatat tgaagtattc ccatacatca ataactttag ctatgactca gatgatgaat
     9481 acacgagata cacaggccaa aagctaggtt cgatggattt tatctactat ttcaaaaaag
     9541 acccgaaaat cgacaaagtt acaggaattt atagcgaaat tgaccgactg tacggaattg
     9601 ctgatgattc cagtttttca agtgattcaa acagaggtga taaggacctc gttgctgttg
     9661 tcgaaaaact aggaagtgag gccagtggac catatgcaag tcttaaagtc gttgatatac
     9721 ccgatggggc tgagtgggaa ataagtgatt atgacggcgt tgaaactgca cattatggtt
     9781 ttcaaacagg tagtatttag tccactaatg acaactaaag aggaggcaga aggatgagtg
     9841 aagatgaata ctttgaacag tagtctatta acgacagaga tttttaactg gaaaggggtg
     9901 tagtcatgga cgcatcggaa gtagtaaata aaattaatag tctcaaagag gttttcggag
     9961 acgtcgaagt agtgataaac gcacaaggct atgacaacgg attttttaag cagattaccg
    10021 acattagcat tcagcaagga atggaagacg aagatggaaa ctttatcgat gaagtagcca
    10081 ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg aggcagaagg atgaaaaaat
    10141 tgcaccaact catatctgag aaagaaagcg aactccaaaa tttagaagat agtttgggat
    10201 tggggtttcc aattgttgag caagctaaaa tgactcaaat cagtcatcta cgacttgaat
    10261 tggaagattt aagacaaata gaaaaatcaa ttcaactcaa cgacaaccag aaaatcgtgt
    10321 tggagtggtt gaaaagcgag acaattttaa ctagagaagc accaatatta tctgttaatg
    10381 ctttttctga taaaaattta ctaggaaaat tacctgataa agtacgcaaa gcttataaac
    10441 tattggcttg taaacaagaa tatgaagtcc tatcagcatt cgctcaatgg ggattagaac
    10501 aggaggaagc ggaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
    10561 gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag aaacatataa
    10621 agtggcaagt cagtttgttg ctgatgcgag taatgctatt cgtaagtttg ggatttcgat
    10681 attaaatgat gatcgtaaat gggaggaaat gagatgaaaa taaaagacgg attttacgct
    10741 agtagtcatg gtatcggcgg tttaatgcta gacatgccga taaagaaccc taaaacacgt
    10801 cagaaatcaa aattcaaaat cggtgacatg gtccgatgta aagcagaaga gttcatctat
    10861 ccgtttcgtg gatatgtaga gcatgtctat aatcattcag caatcattca cattgaaaac
    10921 acgatggaat gcgataagtg gttagcgaaa agcaaagata atttagcagt ggctcgtttg
    10981 gtggatatgg aactaatcaa tgacaaataa aaaagccgga tcgctccgac tgattcaata
    11041 aatccaacac atttattata tcacataaag gagcggtttg acttgatgca attgttacga
    11101 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
    11161 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg
    11221 ccgaaggcac cgaagcacgg caataaggca gaagacgcga tcattcagat gatggatata
    11281 gaagcggaga gagacgcaat actagcggct ttgatggctc ttagtctgat tagccgtcag
    11341 atactctact acagcttctg tgacgtaaac aagcactcta attatgaaat agggcaattg
    11401 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt aatcgaattt
    11461 gcagaagcat acaaaaaagg cgtgttagtt cagtatcgtt gattttgtag ggtttttgta
    11521 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
    11581 tgataggtct gagacaaaat aaaatgtaag ggaggaaatc tccctcatcg ttttaactta
    11641 agcttcgata gacagcaacg gaaatattaa gaataaggat gtgaatttca actccttcta
    11701 aattgttctt attatctatc atccgttgtt gtctattaat ttatgcatcg gaggaaaaac
    11761 gaatggataa agaaatcaaa gtaactgtca aattagattt gactgaacta aaagaactgc
    11821 tcaacaaggc tagtgaccaa gtcgaacagt tacaagaaac tttagatgaa attgctaatt
    11881 ttaaaatcca agtttcttag cagtatattt tttagcagct gcactcataa aatcatacca
    11941 agtatcagaa aaatataact taactaaata attaaaaaag gtgggtaaat agaaatgacg
    12001 gcgttaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg atttaaaaat
    12061 ggacaaaata aaaaatagta gcagccgaga ataattttat agtgtcactg tggcggaaat
    12121 agaagacgca aaggtggagg taggaagcgt atggcgcact atcgggacct tgtacaaaac
    12181 tagaaaggac tagtacgtgt gtggtgcgag ccctagggag gaacaggata accgcctgtg
    12241 tgtaggttgc tattacatat tagatcactc tttgagtggt ctttttattt acataaagga
    12301 ggctacataa tgagaaacta ctggtatata tcgctaacta atgaatatcc tcgaaccatt
    12361 gatgattgtt cagtgcgtgt tgtgcgttct gtccaaatca aaaagaagta ctccattgtt
    12421 gaaatgacca gagaagctac gccaaaagag attgataaat gcaaacttct ttattgcggt
    12481 catggtttct atgatgaacc aaacattcaa aataatatta acaagaattt gagggattag
    12541 aatgcaagaa atggcttaaa aatctccaat caggacaaat caagttagac aaagtttccg
    12601 atttaaagat attgattgaa gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa
    12661 aactcaacct aagaagattg cgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa
    12721 gacgtgatga agccaaaaga atttggttag aatccaacgg agaaaagcag ttaaaggaaa
    12781 ttgcatctga attaaatgtt tcagattctc aggttagaaa atggaaatcg caagacaaat
    12841 ggagcgctga attgaaaagt aacgttacca atggcaaaag taacgttact aatcaaggtg
    12901 gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg aaacagcaga gcatcaccgc
    12961 caaagagaaa taagaatgct cttaaaactg gtgagtatga aacaatattt tttgacacgt
    13021 taaccgataa tgagaaggac atctattcta gtttgaatga tgatccttct tttgctttgt
    13081 ccgaagaaat acgacttctt aaaataagac aacttcgaat gatgaaaaga atccaacaag
    13141 ctgaagctgg actaaatgat gaagaagtcg aacgattgca gcagctaaga aagattaaaa
    13201 ctccgattga aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg caagatgtgc
    13261 agattagcag aaaaaaacat cgcaaaattg atgatattct ttcaattgaa gattcattga
    13321 ctcggattag caaccagtta gctaaagcca tcaagcaaat gaatgaactt tatatgaatg
    13381 aatacagaac tgatttaatt aaagctcaga ctgataagat ccaagctgag acaaatgaaa
    13441 ttggcggaaa taattcaggt gaagaaatag aagaatggaa acaggcagtt ttaaatgccg
    13501 caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc tgatattggt
    13561 tctgccattg attattacta tgataaacca gtagcttttt gccaagatat tttgcatttg
    13621 aatcctgatg aatggcaaga aaatgtttta aatgatttag ctgaattttc aaaggtttct
    13681 gttcgttctg gtcaaggagt tggaaaaaca gcattagaag caggagcaat actttggttc
    13741 ttaacgtgtc gaccctacgc taaagtaata gcaacagctc cgacaatgaa gcaactttac
    13801 gatgtacttt gggcagaggt agctaaatgg ttaaatgata gcttgatcaa aaacttactg
    13861 aagtggacaa agaccaaaat ttatatggtt ggtgattcag agcgttggtt tgctacggct
    13921 agaacagcga ctaaaccaga aaatatgcaa ggttttcacg aggaccatat gttgattgtg
    13981 gtagatgaag cttctggtgt gtctgatcca attatggaag ctattcttgg tacgctatca
    14041 ggttttgata ataagctgtt gatgtgtgga aaccctaata atattgaagg tgtcttttac
    14101 gattcccaca attcagaccg tgataaatac agagttcata aagtatcaag ctatgatagt
    14161 aaacgtacaa acaaagacaa tatagaaatg attcttaaaa aatatggaaa agaaagtgat
    14221 gttgctcgtg tccgtatttt tggagaattt cccaaaggtg cgttggattc atttatcagt
    14281 cttgaaacgg ttgaattggc tacagaaaaa caaattagtg attctttagt caataaaaca
    14341 acggttgctc atattggtgt tgacgtagct cgatatggtg atgattctac gattctcttt
    14401 cctagaattg ctaccagggc attggagtat gagaagtatt caaaacgtag cactatggaa
    14461 acaacaggat atgtcatcaa catggccaag aatctaatga gtcaatatcc gagtattgat
    14521 aaagtgatga ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa
    14581 gaacttatag aagacaagca ttatcctttt gaggtgtttg gagtgaataa cggttcaaca
    14641 tcagaagacg atttttacga taatttaggt actcaactat ggggaaacat caaggaaatg
    14701 ttagaagaaa atatgacagc aaatcttaac ggagaacagc ctgttattga attgccttct
    14761 gatagttcgt taatcaaaga attaagtact cgcaaattca aaatgacaag tagaagtcgt
    14821 atacgtttag aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc cgatattgct
    14881 gacgcactgg ctttagcgtt ttatgagcca ccaagtcact atcaatttat tcaattttag
    14941 gaggtgagtt tttgattagt acagtattat ctttagagag atataagaag ctacgtgtta
    15001 aatatgcaac acaaattgaa gatggaatgt ttgatccgaa tggttttata gaatatatga
    15061 aaccattttt tgctgatcgc gaaagaaaat atctagctta tactagcgaa aagaatgaaa
    15121 tagacaatag accaaagcct aacactgata ttgtaaaagt taataataaa cttcatgctg
    15181 gtatgtattc aattgtcgta gaccaagcag tcaatcattt tactggtata cctatcaaat
    15241 gggattacga tgtatcggaa caaaaaagaa cactcattca aagattaaaa gataaattct
    15301 taaaaaatga tatagaactt ccaacagttc cagaagcttt taacaaatta acaagcaatc
    15361 ttgattccat gagattcgca atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg
    15421 gagtagcttt ccgattgtta gagcctgtgg aagaagatga tggttggaag ttaagagcaa
    15481 gtaatattga accttggaga gcagaaagat atggaaatgc cggaatctat atcaaagaaa
    15541 agtatgactc ttaccagaaa aaattttttc aagaaatgaa agttatgaca agaaataaaa
    15601 ttctgacata tgcttgctat ggtgatttga attttgttac aagcggaacg ttcaaaaaaa
    15661 ttgatgagac cgataaccct ttggggacga ttaccttggc agaatttaaa aataatacga
    15721 atcgttattg tgattttgaa gtagctgagg aaattggaga tgcaattgat cgtgctttgt
    15781 ctgatcaaca aaacgaaatc gaacaattta agcttgcgta catgcttgtt actggcacaa
    15841 caatgagcaa aggcactgct aaagaaatga tgaatcagct agggattatc aacttaaaag
    15901 accctactgc aaaagctgaa tatgtcacaa aaaaattagc aaaagatttc aatgaatatc
    15961 atatggatct attgaaaaaa cagttttaca ccatttgtaa agcaatcgat tttaacgatg
    16021 aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt agcctagaag
    16081 cgaaaacaaa tactaaagag caatatttta gagaaggatt gaaagaatgc gcagaaacaa
    16141 ttgctgcttt tcttaagttt catgacaaag tagaaattga gcctgaaaaa attattttta
    16201 ctttctctag atctttgcca actgatttaa gttatttagc tgaagcttta cctaaacttg
    16261 caccatacgt atccaaacga actattcaga gccaaattcc atttgtaaca gatgttgatt
    16321 atgaaaatga aatgatggaa ttggaaagtg gaagtgctta tccagatagt gaatacaatt
    16381 ttggcggagg tggcaatagt gacgatagaa acgaagtatt ggaccaaacg tcgagaactg
    16441 gaggatcaag caaggctcaa acaagaaaat cagacactta aaaaattaac tagtgtattt
    16501 cctgaagcac tgaaagagat acaagcaaaa ctattatcac aagctgactt acacaatatc
    16561 acttatccag aaatgatgga gttttacagt acaagtaatc agaaaaaata tcgtgaatat
    16621 gtggaaaaaa attataagtc attaaaaatg tatgatgcaa aatacaaaga gtttatcgat
    16681 gaattttttc caccatttga ctatgcaaaa gtcaatcgct tattacaaat acgatcagat
    16741 gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg tgaatcaata tttttctgat
    16801 cgcttagagg aaattcttca aagaacatat tcttctaatg ctaatgtttt tgttcaactt
    16861 ttaaacgttg atataccaaa ttatttacca gaaaatgaaa ttaaacatta tttgaactat
    16921 ccatggtcag gaaagacatt ttcaagaaga ctttggggaa atatttcatc gcttgaacag
    16981 aaactctcta atgctattgt aaaaagtgtt gctagtggag aaggcgttat acacgcatta
    17041 aacaccatga gattagactc agaaatttgt gacatgttta agttagaaga atcaaagtat
    17101 aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca agatggtatt
    17161 gaaaaatcat atttagaaac aggtattgag gaatacaacg tattgactgc aaaagatgag
    17221 agagtttgcc ggatttgtgg aggaaaggca agtaagaatc cctataaact gaaagatgct
    17281 gtcataggtg aaaatcgagc acctttccat agtcgttgta gatgtacgga tgttcctaat
    17341 ttaccaaaat taggaaagga tattgatgaa gaatatgacc gtttatttgg cgatttatta
    17401 gatgagtttg cacatgattc ttttggaatt aatttgaaac ggaggaagta gaatgaaaga
    17461 tttttttgaa gcagtactaa caattaatgt aaatgctgat attgcagaag cctacaaaac
    17521 agctattgag tctgagaacc atcctaatgg cttgagagac cattggaatg gcaattatgc
    17581 ctacgtggtt attggcgatc aaactgttaa ttatcaagat aatactccag ttgataagaa
    17641 taccgttaat ttaacgattc aattattatc tcattcatta ccaaatttaa aagaaacagt
    17701 tgattggtat gaaaagatgg gatgtattgt tgttagaact gactacaaag aaggaaagtc
    17761 tagtaattag gcttttttat tttgtccgaa atgacgttaa actagcgcaa tactgggctt
    17821 ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc
    17881 gtggggcgaa aggagaatga gcatgaaaca caaatcatta atgccaatga atttgcaata
    17941 ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc
    18001 caatgaagaa gctcaaaaat ttattcagtc acaatctcaa agtgttgccg acaaacaatt
    18061 ggaagcttgg aaacaaaata acttagataa aattaaacag gataccatca aggaatatga
    18121 ggaatctaag aaaaacaagt cacctgagca aattcaactg gaaaaactac aagctgaatt
    18181 tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa gcatttgttg cagaacaaat
    18241 tgctggatta gaactagatg gagagttaaa agagtctatt tctcaattta tgctaaataa
    18301 tcttgttagt tcggatacag atttcactaa gaatgctgtt gaaggtttta caagtgtttt
    18361 gaatgcaatt aatgagaaac atgcagatgc attaaaagaa ctacaaatga agtctgcatt
    18421 tggtggaact caacaatcga ataaccaggt tcagcagaac aatgaaacat taacaaatcc
    18481 agaagaacaa ttaggacaaa ttcttcaaca atttaactag gagagtgaaa aattatgaaa
    18541 aaaacatctt taaataattt agagtatttg gatatttcac cagcgattaa tgctatgcaa
    18601 gtaccaaata cacctttttt aagctattta cttggtgctg gaaaaacaga gccagcaaac
    18661 tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg atgattcttc tgaaaaactt
    18721 gagggcggag aatatccaga tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa
    18781 atttttagaa aatcaacctc tgtatctggt acattagatg ctattaatgt gaatggagtc
    18841 ggaaatgaat taactaatca agtagcacta cgtggtatgg aaatgaaaat tgatttgaac
    18901 cgaaaattga ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat
    18961 gggattttga acttgatcaa ttcagcaaat aaggcagaaa cagccactgc gggtgcagta
    19021 acaagaaaag atatcgatac tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa
    19081 aaactatgct tgatttctcc agatatgcag gagttaatga ctgatgagtt agatggaaaa
    19141 tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg gattgcaaat tggaaatatc
    19201 gtgtctaatt acggtacagg tattgctcta ctagaaccat cattgccaaa aggaacaatt
    19261 gccgcaatcg atactaatta tgtgaaacta cgtccattac gtgaatggag agcagaagaa
    19321 cttgcaaaaa caacggattc tagacgtatc ggtcttgtag gtgaatactc tcttgaatac
    19381 aatgcttcaa actctggggc aattttaaat ttaaagtctg aataaaaggg agttagtact
    19441 cccttttttg ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt
    19501 cgtgaaatat agagtaggta aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg
    19561 tagatttatc acagcagatt caaataacga atttatcatt tctatagatg ataaagaagc
    19621 aattgcaatt ttggaagatg caattgatgt taatgaaatt taggaagtga tctgatggat
    19681 gaatcgctaa aaacggaaat cattgagtct acaaaagaag attttccaga tttgagtgaa
    19741 gaacgcataa ctaatttatt agaaataatt ttgctagaaa ttgaatcata caacacttgt
    19801 aaaaatgata tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata
    19861 ataaaaaatg aatcagaaaa aacagtatct tcagttagac gtggcgatac aacgattagt
    19921 tatgcttcaa caacaaatga tgttagtgag ttacttctag gctatggtga tttgatacga
    19981 agagttattg gttgtggagg attggagttt ttttaatgaa tgaagcagat attttagaaa
    20041 ctacttatga agatagttgt attattgaaa gactgacgga cattgaagat tctaatacaa
    20101 atattactat tcaagattac aaaaaagtat atgataatcc tatttcttgc gctctttcac
    20161 aaggtcaaat cgatggacta gcagtcatag aagatggaga gatggtaaat gtttcaactg
    20221 acacatataa attatttgtt catcctaaga ttaaactaaa aaaaggagat cgaataacaa
    20281 taactcaaaa ggccagtggc ttaatttttt ctctatttgc tactaagcct ttttactatc
    20341 ctagtcattg tgaagtgaat ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa
    20401 tcaaatgcag aaaagatcat tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa
    20461 ggaatatcct tcgttaacga ttcaatgaat aaagtcgtta gtttagctaa accattaact
    20521 cctgtaaaat caggtaattt aaggcgagga tatcgtgtag taaaagctag aaaactatca
    20581 agcggtcgaa ttgttggagc agttataaac aatgaacatt attttaaata tgtagaagaa
    20641 ggtcatagga ctaaaaatgg tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg
    20701 aatcttgcaa atatgtctta tattcctcga agatttaaac aaatggcaat aaaaatcgtt
    20761 aagaaaggaa agtaacatga aagatgaaat cattgctgca atcagtagca aattaaaaga
    20821 aatctatcct gatgggacaa tatatcttga ttcagttatg cagtcaacta aagattttta
    20881 tttcgtttta tcagtaatgg aatctggaac tgaaaatgta ggaattgata ttcaaaatgt
    20941 ttctttctta attgatattg cattgattga taataaacct aatagaaatt taattaatga
    21001 attagtctca cgttgtggga ctttttttaa tacgattaca atcgatgagc aaatattatt
    21061 tccaaaagcc tatttacctg atgaagcaga tggtgttcaa catattcgtt ttacattaga
    21121 atttccacaa tatattgaat ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg
    21181 aattatttct gtcgaaaaac ctacttggtt tcctttggaa gatgagacag ggaattttcc
    21241 ggtatatggt actgcgttgc caatgggaac agcagtaagt atcaaaccaa cagctaatta
    21301 tgaaacaaca caagattatg gggattcagt ggttcaagat caatttacag cttttggtgg
    21361 tgcagaagtt gaactggaag caaatggata tactcataaa gttttatcta caattactgg
    21421 agggaaaatt gttaaaggtg gtgccttgcg ttctggagaa gatattgcgc aagatggtgc
    21481 gtttgcatat agacgtaaaa aatcgaatgg gaaataccgt tatacggtct tttataaagg
    21541 acagtttgct ctagattcag atgaaacatc aacgattgaa ggaagtaaag taagttttac
    21601 tcatccaact tggaagggtt catttgtaga tgtaccagga cttggatata tgtattcagt
    21661 tgatgaagat gatgaaggcg tggataaagc aatgatcgga aattggttta caaaagtagc
    21721 aattccaatt gaagaagcag aactttcagg aggtacagaa taatgtcaaa atatcaaaca
    21781 acgattaaat taacgaaaaa agatgaagaa ggtaaatatg agcaagtaca atttaaatct
    21841 gctgaatttc taccaggccc agtcgtagaa gaagcagcag aagtaatgga gggaatgcaa
    21901 aatgctgtag atggaaaatc agtatcagaa gcattaagtc gagcttattc atttattgca
    21961 gatattttgt ttgaaggaca gtttactggt gaagattatc gtcaagggat cgatgctcga
    22021 gaaattgctt cgttgacagg taagttatta aaatctgtta ctgcaggctt tgatgaaact
    22081 tatacagaaa cgaaaaaaaa gtaagcgaag ctctcaaata tccttttttt aaacattcga
    22141 ttacttatcg agaattagat ttaaagacgc aattactcga agcaggttgg acgttaccag
    22201 aaattgaacg tactgacttt gacgagttaa tgcgtctttt tgcttttcga gatgcagtaa
    22261 aagagcatga ggatgttgag tactacgata acttcaccca attttaggag gtgatacttt
    22321 gaataacgat gacctaattc tgaagatgat actagatgaa tctggtttta ctgccggtat
    22381 gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa
    22441 tagtggcagt tcattaggta gtatttggaa aatatttgct ggaagttttc tagctagcgg
    22501 agtaactaga atcgtaggag ctggctttga tctaatcaag ggttccataa gtggagcggt
    22561 tgatcgagta gatacgatga ataacgctct acgaaatttc caaaatatgg gatttagtaa
    22621 ttcagaaatt atgaagaata tcggaaagaa tgggctttta tctcaaggta ttcaaggact
    22681 tcctactgct ttgaatgatg cgataagtca tgttcaactt cttgcttctt ctacaggtga
    22741 tttaactcgt tcaactcaaa tatttaaagc tttaaatgat ggaattcttg gtttcggtgg
    22801 ttcaactgat caagttaatg aagctgttat tcaattatct caaagtttct caaacggaaa
    22861 agtagacgca caaacttgga actcaatgat caacgctcaa cttggaccta ctctttctgc
    22921 tattgctaaa aagatgggaa ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa
    22981 gatttctgtt gaagaattcc aaaatcaatt aatagaaatg gataccaaag gtggcggagg
    23041 acttaaatca ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa cctctataca
    23101 aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta atagaaggat taaataaagc
    23161 attagtagat tcagatttag gtggatttaa gggaattatt gataaagtgg gcagttcaat
    23221 ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa
    23281 tttatttaac gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc cctttcttgt
    23341 tcctgcagct accgcttttg gagcgttcat gtttcaactt aaaggtatac cagcaataat
    23401 aaaaagcttc aataatttta agaatgccat aattggcgtt ggaaattcat taaagattat
    23461 gggggcaata gctgctgcta attcatttgt tttaattatt ggtgcagtcg ttggtgcaat
    23521 agctgttttc ggttacttta tggcaaccaa tgaagagctt agaaacaagg ttatatctat
    23581 ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg actggggaat
    23641 tgatacttgg aattctgcta aataaatggc atcaaatgca gttgagggtg tcaaagatgc
    23701 ttggtcagga acaaaggaat ggttttctaa cacctggaaa ggtattaagg atggggccac
    23761 aggtttattt gataaaacag tagagacttc aagaaatgca gtcgatagtg taaaaaatgc
    23821 atggtctaat atgaagaaat ggttttctga tacttggcaa agtataaaag attcagccag
    23881 agaaaaatgg aatggaatca aaggttctat tatgtcagtt gctggtccat tgattacagg
    23941 tataaagaat gcatttttac atgttacttt ttatctagag accttatgga ataatttagt
    24001 tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt
    24061 tattacctcc atgatttctg gtggatggga agaaacaa
//
LOCUS       DAYYAJ010000034        23827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000034 DAYYAJ010000000
VERSION     DAYYAJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.034"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHATZ_001154"
     CDS             complement(53..787)
                     /locus_tag="ACHATZ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8403698.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHATZ_001155"
     CDS             1114..1524
                     /locus_tag="ACHATZ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8403699.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHATZ_001156"
     CDS             1517..2218
                     /locus_tag="ACHATZ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8403700.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHATZ_001157"
     CDS             2218..3804
                     /locus_tag="ACHATZ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403701.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHATZ_001158"
     CDS             3823..4044
                     /locus_tag="ACHATZ_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8403702.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHATZ_001159"
     CDS             4041..4802
                     /locus_tag="ACHATZ_001159"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8403703.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHATZ_001160"
     CDS             5182..5691
                     /locus_tag="ACHATZ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8403704.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHATZ_001161"
     CDS             complement(5766..6305)
                     /locus_tag="ACHATZ_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8403705.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHATZ_001162"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHATZ_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8403706.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHATZ_001163"
     CDS             7318..8169
                     /locus_tag="ACHATZ_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8403707.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHATZ_001164"
     CDS             8197..8832
                     /locus_tag="ACHATZ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8403708.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHATZ_001165"
     CDS             8852..9625
                     /locus_tag="ACHATZ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8403709.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHATZ_001166"
     CDS             10033..10830
                     /locus_tag="ACHATZ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8403710.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHATZ_001167"
     CDS             10808..11221
                     /locus_tag="ACHATZ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8403711.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHATZ_001168"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHATZ_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8403712.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHATZ_001169"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHATZ_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8403713.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHATZ_001170"
     CDS             15998..16558
                     /locus_tag="ACHATZ_001170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8403714.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHATZ_001171"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHATZ_001171"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8403715.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHATZ_001172"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHATZ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8403716.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHATZ_001173"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHATZ_001173"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8403717.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHATZ_001174"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHATZ_001174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8403718.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHATZ_001175"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHATZ_001175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8403719.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHATZ_001176"
     CDS             21905..22483
                     /locus_tag="ACHATZ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8403720.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHATZ_001177"
     CDS             22535..23017
                     /locus_tag="ACHATZ_001177"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8403721.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHATZ_001178"
     CDS             23134..23508
                     /locus_tag="ACHATZ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403722.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23621..>23827)
                     /locus_tag="ACHATZ_001179"
     CDS             complement(23621..>23827)
                     /locus_tag="ACHATZ_001179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403723.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7780 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DAYYAJ010000035        23250 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000035 DAYYAJ010000000
VERSION     DAYYAJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.035"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..1047
                     /gene="ccpA"
                     /locus_tag="ACHATZ_001180"
     CDS             46..1047
                     /gene="ccpA"
                     /locus_tag="ACHATZ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8403724.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1384..3729)
                     /locus_tag="ACHATZ_001181"
     CDS             complement(1384..3729)
                     /locus_tag="ACHATZ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8403725.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            4008..4910
                     /locus_tag="ACHATZ_001182"
     CDS             4008..4910
                     /locus_tag="ACHATZ_001182"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8403726.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            5123..6898
                     /locus_tag="ACHATZ_001183"
     CDS             5123..6898
                     /locus_tag="ACHATZ_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8403727.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6964..8550)
                     /locus_tag="ACHATZ_001184"
     CDS             complement(6964..8550)
                     /locus_tag="ACHATZ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8403728.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            8801..9025
                     /locus_tag="ACHATZ_001185"
     CDS             8801..9025
                     /locus_tag="ACHATZ_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8403729.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9166..10917
                     /locus_tag="ACHATZ_001186"
     CDS             9166..10917
                     /locus_tag="ACHATZ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8403730.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            10917..12701
                     /locus_tag="ACHATZ_001187"
     CDS             10917..12701
                     /locus_tag="ACHATZ_001187"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8403731.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13027..13878
                     /gene="licT"
                     /locus_tag="ACHATZ_001188"
     CDS             13027..13878
                     /gene="licT"
                     /locus_tag="ACHATZ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8403732.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            13974..15854
                     /locus_tag="ACHATZ_001189"
     CDS             13974..15854
                     /locus_tag="ACHATZ_001189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8403733.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(15894..16796)
                     /locus_tag="ACHATZ_001190"
     CDS             complement(15894..16796)
                     /locus_tag="ACHATZ_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8403734.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            17027..18430
                     /locus_tag="ACHATZ_001191"
     CDS             17027..18430
                     /locus_tag="ACHATZ_001191"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8403735.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            18430..19863
                     /locus_tag="ACHATZ_001192"
     CDS             18430..19863
                     /locus_tag="ACHATZ_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8403736.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            20008..20934
                     /gene="trxB"
                     /locus_tag="ACHATZ_001193"
     CDS             20008..20934
                     /gene="trxB"
                     /locus_tag="ACHATZ_001193"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8403737.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            21148..22875
                     /locus_tag="ACHATZ_001194"
     CDS             21148..22875
                     /locus_tag="ACHATZ_001194"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8403738.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            22890..23240
                     /locus_tag="ACHATZ_001195"
     CDS             22890..23240
                     /locus_tag="ACHATZ_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8403739.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT         7357 a         4145 c         5001 g         6747 t
ORIGIN      
        1 gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga aaagcaaaca
       61 atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc
      121 gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga agttatcgat
      181 cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca
      241 gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc
      301 gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac
      361 caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc
      421 atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt
      481 gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta
      541 tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt
      601 gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg ctataaagaa
      661 gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac
      721 ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac agctgtctat
      781 gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg tggcgtgaaa
      841 gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt
      901 cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta
      961 ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga
     1021 atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt tgtgtgcctg
     1081 ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg tttgatgaat
     1141 attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa aaggggttat
     1201 gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat
     1261 tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca
     1321 aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt gtattctatt
     1381 aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg cataagctga
     1441 agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc
     1501 gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg gtgtttggat
     1561 ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt
     1621 cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc
     1681 ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct
     1741 gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga
     1801 gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc
     1861 ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc
     1921 attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg
     1981 gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat
     2041 gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt tggtctgttg
     2101 aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc
     2161 cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt
     2221 caacacatct tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg
     2281 gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt
     2341 ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc
     2401 catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc
     2461 tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta
     2521 gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc
     2581 atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat agacatcttt
     2641 atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg ccttttgata
     2701 ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg
     2761 tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg
     2821 ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta
     2881 ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc
     2941 aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac tatacgtgat
     3001 cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc
     3061 tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc ccttattatt
     3121 acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt
     3181 ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt
     3241 aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc
     3301 agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc
     3361 gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa tcttatcaga
     3421 tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata
     3481 aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg
     3541 caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc
     3601 tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat
     3661 aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt
     3721 gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag
     3781 aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag agttaaaacg
     3841 tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga aaatttcagt
     3901 tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat
     3961 atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct
     4021 cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagcagaa gcagccagtt
     4081 atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca
     4141 tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaacggt ctaacaaata
     4201 tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc
     4261 cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc
     4321 aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa
     4381 ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga
     4441 caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg
     4501 atggagtaaa attaaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg
     4561 ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg
     4621 ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata
     4681 aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg
     4741 tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat gtcggcaaag
     4801 ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcacaaatat
     4861 tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat
     4921 ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa
     4981 ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga
     5041 caaacaaggt atactgttag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa
     5101 acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa
     5161 aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta
     5221 tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct
     5281 gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac
     5341 actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc
     5401 ttatgctgtt ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt
     5461 taaaccgatc gtgaagaaca agtatcttct tttaagtatc tatttttatt ttttgttttc
     5521 tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc tcgtgattcc
     5581 ggctgttgag atcattatcg cttgtttact agctatttat taccgcaaat acggttctag
     5641 cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat
     5701 tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt
     5761 ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact
     5821 ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg
     5881 cttttccatc gtctatcagt ggatcgaata taaaaatgag acaacatacg gtattgcaac
     5941 catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga
     6001 tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa
     6061 taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata
     6121 tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt
     6181 ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac
     6241 caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaaccat
     6301 caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct
     6361 gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt
     6421 ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt
     6481 attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt
     6541 ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc
     6601 tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt
     6661 tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact
     6721 gacacaattg gtttttcttt tcggtgtctc gtggttttgt tcttatgcgc cagaaaaaaa
     6781 ggattatttt ttaagtgtct acactggcgc aggcgtgcca ttaacgcaat tcatcgtagt
     6841 aacaagcatg ctagttttca gtaaattaaa tctgaaaaag gctttatctt ctaaataaaa
     6901 agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag
     6961 tttctattcg atttcttcta ccgtttcctg caaacgctgt tcatattttt tgtttccttt
     7021 atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga ttacataagc
     7081 gatgtgatca gccaatgcta gttgagcaga agtggcttgt tcaccaaaga aactttcgat
     7141 ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga accagccaag
     7201 aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat
     7261 catcagtaaa gggatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt
     7321 catcaagtta ttcaaagcgg tatgttcttc gatcgttcct tttgtacttg taaacaaaac
     7381 acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg
     7441 gatacgcata tggatgaagc cttccatgat gacttgaccg gtcaacgtac cagtaatagt
     7501 agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggataaga taccagattt
     7561 tgctacagca atcaagatac cattgctcat agctgatgga tcagataagg cttcatacag
     7621 accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac ccatgatcaa
     7681 tagcagtgca ttgactagaa aagccatcga caattggata ttagagtccc aagtagaaaa
     7741 acgtacagct tgtgctacat cttcttcgtc ttggtgatca atcttccgag tttgagaaac
     7801 tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc ctaaagctcc
     7861 agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac tgttagggat
     7921 aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac
     7981 gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa acccgacttt
     8041 tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg
     8101 aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcga tgtctgttgc
     8161 cataatggct aattcagtca agatccataa aatgacaccc aatgtcttac ttgtgcgtgc
     8221 tcggattgct tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatactg
     8281 taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt
     8341 ttgtccacca gtgatcgaag tggaccagtt acctggatcc atataaccaa ctgctactaa
     8401 tgcacctggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc
     8461 aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt
     8521 atgaaattgt ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga
     8581 ttacattcta cacctcaaaa agaaaaaatt aaagaaaaat attcggtatg cctaatttat
     8641 tttcgtggcg cgtcttgtta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact
     8701 attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa
     8761 gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt
     8821 cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg
     8881 taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc
     8941 aatgatgatg tcaatgggac aaaaaccatc tgagaagaaa attcgccaga tgatgcagca
     9001 aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt
     9061 tcagtccgtt ttttattttg taatttgtta gactattcag attattcagc aaaaagacaa
     9121 gcacagacaa tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga
     9181 aagttaggct ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta
     9241 ttcgcagtgg ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag
     9301 attgccgata aaaatttatc agtaaagatc attttcgggt ggatcttcct attgattatc
     9361 gctgctttct tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca
     9421 gctcggttgg agaaagaact tcggcagcag ttattcaaac attttacaaa aatggacaat
     9481 ctcttttacc aaaaatatcg gactggggat ttgatggctc atgcaacgaa tgatttgaat
     9541 gcgatccaga atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc
     9601 ggaacaacga tcattgtgat gattttgttt attgattggc gcttaacact gattgcgctg
     9661 attcctctgc ctttgctggc cgtcacttca cgagtgctgg ggtcgaaact ccatgatgct
     9721 tttcgtgatt cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact
     9781 gggatcaaag tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa
     9841 aagattgaag atgcaattat taaaaacaaa cgagtcaatt ttctagactc cttatttgat
     9901 ccattcatta ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag
     9961 tatgtcttgg aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt
    10021 atgcttgttt ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat
    10081 gcttcttatg atcgcgtcaa tgaacttttg catgaaaaaa cgcatatcgt ggaacaactg
    10141 ggtgcagtgc agaccccggt aaaaggcgag ctgaatgtag ctatttcctc ttttaattat
    10201 ccgaaagaag aatcaccagc attagaacaa atcaagtttg caatccaaga aggagaaaca
    10261 ttaggtattg ttgggaaaac tggtgcggga aaaacaacga tcatcaaatt actgatgcgt
    10321 gaatacgatg attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca
    10381 ttgaatgctt tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg
    10441 accattcgag ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat
    10501 gcggcttctt tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc
    10561 ttagttggag aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct
    10621 cgagcattga tcaaaaatcc tgaattatta atattagatg atgcactttc tgcagtagat
    10681 gccaaaacag aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact
    10741 atcattgcag ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat
    10801 caagggaaaa tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat
    10861 gaacaaatgt gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg
    10921 atgaaaatta tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca
    10981 tcaaacgact gatgaagttc gccaaacctt ttagaacaac gttcatcgtt gctatcttat
    11041 ttgcgtttgc tctgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg
    11101 atgatcatct ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt
    11161 acttatttgg agtcctagta aaaagtatca tttggttttt ccaatggtat ttgtattcca
    11221 tggcttcatt gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac
    11281 taggtatgcg ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg
    11341 acacagaaac gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg
    11401 cagtcgtttc ttcattcatt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt
    11461 tggtgttctt gccgatttta ggaattgtca tctggtatta ccagaaattt agttctcgta
    11521 tttaccgaaa tatgcgggaa aaattaagtc agctgaatac aaaattgaat gaatcgattt
    11581 ccggcatgcg tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac
    11641 aagtgaacaa tgagtattta gacacgcgct atgcgatgat caaaacgaat tcgattctgt
    11701 taagtccgat catcaatttt ctatatgcat tggcgattgc gttggcgttg actttatttg
    11761 ggatcgatgc gttgcattct cccgtagaag ttggactgat ctatgcattt acgacttatg
    11821 tccagggatt ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg
    11881 ggatggttgc tggtagtcgt atcttgaaga ttatggatac ggaagaatat gcaccacaac
    11941 aaaaccctgg agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct
    12001 tttcttatga tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag
    12061 aaaccgttgc attagtggga cacaccggaa gtgggaaaag ctcgatcatc aatgtgttga
    12121 tgcggtttta cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat
    12181 atccaatgga agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt
    12241 atggggatat cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac
    12301 gccatgcggc tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc
    12361 actcaaaagt gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt
    12421 ttgcgcgaac aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata
    12481 tcgatactga aacagaagga ttgatccaag aaggattggc gaaaatgcga caaggacgga
    12541 caacgattgc gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt
    12601 tagacaaagg tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt
    12661 tgtatgcaga tatgtacaaa ctgcaaagta gtggtatata aaagatgttg tgacagtagc
    12721 attttgacct aagcactaga gtgaaaataa aacatagctt cccatatttt tcatctgtga
    12781 gagttagtgt cgtagttacc agatttaaaa aagacgacgt cagctagtca gaagtgccta
    12841 atagccccta agaaagcgtt gacaaaatcg tttgctttcg ataatataaa gaaaacgata
    12901 caacttggat agtgattgcc tgatgcaagc taaacctgat gagtctccca tggtttcatg
    12961 gacggctttt tgggtttttc tttttgtttt ggatacagcc ctatatatca tggaaaggag
    13021 caaaacatgc tgattgagaa agtactgaat aacaacgtcg taataacaag ggatgaaaga
    13081 gatcgggaaa tgattgtgat gggaaaagga ttggcattca aaaagaaggt gggtgacccg
    13141 attcttgact catgcatcga caaaaaattt cagttgacaa atgacggact ttcacaacag
    13201 ttccaagaat tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt
    13261 gaatatacaa aagaggctct gcaagtccat ttaaatgatt ctgtgtatat ttcattgacg
    13321 gatcacttgt atacagctat cgaacgtcaa aaaatgggga tctttgtgcc aaatgtattg
    13381 ctgtatgata tcaaacaact gtttcctaaa gagtatgcca ttgggaaaaa aacagtgaag
    13441 aaaatctcgg ataaatacca catcaagtta tcagaagacg aagccggatt tatcgcattg
    13501 catttagtca atgcacaagg cattcaaagc cagcaagaca atacctacct gtttaccaca
    13561 acaatacagg atattttaaa tatcgtccgc tattactata agattacatt tgatgaagat
    13621 tcagtccact tttttcgatt cgtcacacac ctgcgttttt ttctgacacg tgtcctagat
    13681 ggagcgaaat cagaagatgg agaagtttct gaggaattgt tccaagttat tcaaaaaaaa
    13741 tataaggata cgtttggttg cgttgagaaa atagcagact ttttaaccgg ggctttcgac
    13801 taccagatgt ccacggatga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat
    13861 gagaatcgtt gtaaataaat aaaaaaggag gacaaattaa caagaacgta tgaaataagg
    13921 gtagtgacat tcagttggct aaaccttcca ctaattaatt ggagggaata gaaatgggaa
    13981 aataccagga attagcagaa aacatcgtaa agaatatcgg aggaaaagaa aatatccgta
    14041 gcttgacaca ctgtgtgacg cgacttcgtt tcaaactaaa ggaggaaaat aaagcaaagg
    14101 atgatgtttt gaaaaagatg gaagggatag tcaccatcat gaaaagcggc gggcaatatc
    14161 aagttgtaat cgggaatcat gtgccagatg tctatgcaga agtactgaca gtagccggga
    14221 tttccgatga agcagaagaa gaggtttctt caagtagtaa tctgttcaat cgattaatcg
    14281 acatattaag tggctgtttc cagccttttt taggtgcttt ggctgcagcg ggaatggtca
    14341 aaggattaaa tgctttgttc ttgtttctca aattttatac tgaaggatcg ggtacgtaca
    14401 tcatgctgaa tgggataggg gatgcaattt tttatttcat gccagtgatc ttagggtaca
    14461 cagcagcgaa aaaatttcat ctgaatccga tggtggggct cgtgattggt gcagcacttt
    14521 gttatccgac tgtacaagga gcagcactac aaagtggttt ccctgatggc agtcctgcgc
    14581 cttatagctt gctgggtctt ccggcttatg aaacattttt gggtatccca tgggtgggca
    14641 gcagttatac tagtagcgtc gtacctgtta tcttcattat cgcatttgcc ggacaagtcc
    14701 aaaaaattgc caagaaatgg attcctgcag tcgtccagac ctttttggtt tctttttctg
    14761 tgttattaat cgcacttcct gctggctttt tgattatcgg tccgattgtc agtatactga
    14821 cagatctatt aagcacggga ttccaagcat tgatggactt ttctccagct gtgtatggtg
    14881 cagttctagg gtttctttgg caagtactgg tgattttcgg acttcattgg agtattatcc
    14941 ctcttgcgat tatgcagcta gcgcaaaatg ggtggagtca gatgttagta ggcgtttatg
    15001 gcgcgagttt tgcacagaca gcagtggtat tggcgatgta tttcaagttc aaggatcaaa
    15061 aaatgaaggc gctttgcatg ccagctgtga tttcaggaat cttcggcgta acagaaccgg
    15121 ctatttatgg aatcacctta ccgaagaaga agccatttat ttactcgatg attggtggtg
    15181 ctgtttcagg aatctatttg atggcatctg gaacaatcag ctacataatg ggcggattag
    15241 ggatttttgg ggtggtaaac tacatcacgg aaacagacgc cagtggtatg tttcaagcgt
    15301 ttatcggcat tgctattgct tcggtaatcg ggttttcgct gacttttttc ttttggaagg
    15361 atgaagcagg tgaaacagat gctatacaga aaaaatcagg taaagaaatt gtaaaaacac
    15421 cgatacaagg acaaatcgct ccactaaatg cagcgaagga tgcagctttt gcacaaggaa
    15481 cactaggtcg aggaatctta atctaccctg aaaaaggaga agtacgtgcc ccttttgacg
    15541 gaacagttat gaccctgttc ccgacgaaac atgcaatcgg aatggtttct gaaacagggc
    15601 tggaattgct gattcatgtc ggattggata ctgtgcagtt ggaagggaaa tattttgaaa
    15661 gcttagtgca gcaaggagaa agagtgagac aaggcgatct gttgctacgc ttcgacaaaa
    15721 agaaaatcga aaaggaagga tacagtttag aaacacctgt gatcgtcacc aattacacgg
    15781 attatttgga tattttggaa aaccaagggg agacggtcgg gccagacgat gcacttatca
    15841 ccgcactgac ctgaacggtt aggtgaagaa tggactaggc ttaactattc tttttattcc
    15901 acttctttat tttcaaaatt gaaaggctct ttcatgatgg ctgagctgat agggcgttta
    15961 tcgaattttt gaccgacaga aattagatgg actaaattct gttgataatt ttctgcaaaa
    16021 acacaactat ataacacgtc tagaagataa caaattgatg tattgatcgt aaaattacca
    16081 atttttgaat acagtcgctc gcgtgtcgta agtcgcaaag cggctgtact cattgcagat
    16141 agcgttgaat caccgatact ggtcaaagct aaaattggaa catcattttg tttcaatatt
    16201 tctgcggtac gaaggatcac attgttttca cccgtataag aaatcagaat cccgcacata
    16261 tcgctcgtac aattgtacgc ttcatagaat gattcaccaa aagtggtgac aattgtgaca
    16321 tccattttga tgcgcttcat tttcagcatg aaatcttgag aaatcaaagt attcgagtta
    16381 ctggcgaaga tcacgatttt ttttgcctgg agcagtaaat atttagcttt ttgcagttgg
    16441 tcatgatgaa tgagagaaag tgtatcatga atcgtcatct gttctaaaga agccatttta
    16501 tttgcgattg tcataatacc gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga
    16561 aaattccctt caagataggc tgtttcagct aaaaaagcat tacgaaaact gacccagcca
    16621 tcaaaaccca ttttttttgc tacacggatg agtgtagacg ggtggacata acatcgctca
    16681 gctatcgctt ggatggtcat ttcgccaagt gctggagaat cgcttaagag aaactggatc
    16741 acttcatttt ctgaaggaga aaaggagatt tctttgattt tttcacgtaa taacatgtag
    16801 ataccttctt cttatttttt tacgtaaatt ataacttttt tgaaaatgtt ttgtaaacgg
    16861 atttatacta tataacttaa aataggcttt ttttgacaag atatttacaa aaatccacta
    16921 gaaaacagta acttttgtaa atgttactgg aaagaaacgg atttctattg tttgttttgt
    16981 ttacaaaaaa gataatgaaa gtgtaaaaaa gtaaaggagt gagaacatgg caaaaggaat
    17041 caaaatcgct acgattggtg gaggcagcag ttacacccct gagttgatgg aaggatttat
    17101 caaaagatat gaggaattac cgatacgaga aatctggctc gtagatgtag aagaaggaaa
    17161 agaaaagctt gagatcgtta gtcagatgtc tcagcgtatg tgggatgctt ctccatatga
    17221 agtcaagatc tatgcaactc tggatcggga agaagcattg aaagatgcgg attttgttac
    17281 gactcagttt cgtgtcggtc aattagatgc acgaatcaaa gacgagcgga tccctagtta
    17341 ttatgggatg attgggcaag aaacaaatgg tgctggcggg atattcaaag cttttcgtac
    17401 cgttccagtc gttttggcaa tcgttgagga catgaagcgt ttatgtccaa atgcttggct
    17461 gatcaatttc gcgaacccaa gtgggatggt gacagaagca gttatcagat atggcaaatg
    17521 ggataaagtg attggattat gcaatgtgcc agtgatggcg atgatgacag aaccagcaat
    17581 gcttggcaag aaaaaagaag agctgatcta caagtttgcc ggtctaaatc atttccattg
    17641 gcacaaagta gcagataata aaggaaatga ccgcacggca gaattgattg atcgtttata
    17701 cgatgaagat aatggattac cgaaaaatat ctttgatatc ccgtttttca aagaacaatt
    17761 aaaacaaatg aacatgatcc cttgtggcta ccaccgctat tactatcgtg aagaagaaat
    17821 gctggaacat gctttagaag aatatcaaac gattggtaca agagcgcagc aagtgaaaga
    17881 aacagaagca gagctatttg aattatacaa agatccagaa ttagaccata aacctgaaca
    17941 attgcaaaaa cgaggtggcg cttattattc agacgctgcc tgtgaaacga ttgcttcaat
    18001 ctatggaaat aaagaaacac agatcgttgt atctacaaag aatgatggag ctgttcctga
    18061 cctgtcggca gattgtgttg tagaagtcac tgcctatgtt ggcgcacaag gagcgagaaa
    18121 tgtagcgttt ggttctcttc ctgctgctga aagaggctgg ttgcaagtaa tgaagaatat
    18181 ggaattgctg acgatcgaag cagctgtgac tggtgattat gggacagctt tacaggcgtt
    18241 tacgatcaat ccacttatac gatcaggaaa gacagcaaaa cggatcatgg atgagttgtt
    18301 tattgcacac cagcagtatt taccgcaatt caaggaaaca atcagccgat taaaagaaga
    18361 aggaatcgaa gttcaagatg aactggcaag agaattatca ggtaatacac atataatgga
    18421 ggaagtataa tggggttcag aaaagatttt ttatggggcg gtgctacagc agccaatcaa
    18481 tgtgagggcg cttatgatga aggtggacgc ggactagcaa acgtagatgt tgtgccaatc
    18541 ggaaaagacc gttttcctgt tatcaccgga aagaaaaaaa tgtttgaatt tgatgaggat
    18601 catttttatc cagcaaaaat tgcaatcgat atgtatcatc gttataaaga agatattgct
    18661 ttgtttgcag aaatggggtt caagacttat cgaatgtcta tcgcctggag tcgtattttt
    18721 ccaatggggg atgagaaaga gccgaatgaa gctggcttga aattttatga ggatgtcttt
    18781 aaagaatgcc aaaagcacgg aattgagcca cttgtgacga ttacgcattt tgattgtcca
    18841 atgcatttga tcaaagaata tggtgcgtgg cgttccagag aactcgttgg tttttatgaa
    18901 aatctgtgtc gagtcatctt caatcgctat aaaggacttg tcaaatattg gctgacattc
    18961 aatgagatca atatgatcct tcatgcacca tttatgggcg cagggctgta ttttgaagaa
    19021 ggagaaaatg aagaacaaat caaatatcaa gcggcacatc atgagttggt tgcaagtgcc
    19081 atcgcaacga agattgctca tgaagtagat ccggagaaca aagtaggctg tatgctggca
    19141 gcaggagcat attatccata cacttgcaat cctgaagatg tttgggcttc tagaaaatca
    19201 gatcgagaaa attatttctt cattgatgtc cagtcacgag gagaatatcc cgcttatgca
    19261 ttgaaagaaa tggagcgaaa tggcattcag atcgaaatgg aaccagggga tgccgcattg
    19321 ctgaaagaac atactgttga ttttatctct ttttcttatt attcttcacg agtttcgact
    19381 accgacaacg aactcttgga acagactgca ggaaatatct ttgcttctgt caaaaatcca
    19441 tatctgcaag caagtgaatg ggggtggcaa attgatccat taggcttgag aataacgatg
    19501 aatgatattt atgatcgtta tcaaaaacca ttatttattg tagaaaacgg cttaggtgca
    19561 gtcgatacgc cagatgaaaa tggttatgtg gaagatgatt accgaatcga ttacctggca
    19621 gcgcatatcc aggcgatgaa ggatgcagta gaactagacg gagtcgatct attaggctat
    19681 acgacttggg gatgtatcga tttagtatca gctggtaccg gtgagatgaa gaaaaggtac
    19741 ggattcattt atgtcgatcg tgataaccaa ggaaatggca cattagcccg ttcaaagaaa
    19801 aaatcattca actggtataa aaaagtaatt gattcaaatg gagaaaatct ttcgaacaat
    19861 tgatcaatga tttataaaag tctattgttt ttcagcggat ttttcgagta taataaagct
    19921 attcgagaaa cgcaagcaga acaatagact ttttttcgca ttggctgtaa agtatgctta
    19981 cttgattaag taaaggagaa tctacatatg tatgatgtaa tcgttattgg cgcaggtcct
    20041 gctgggatga ctgctgcttt gtatgcttca cgttcaaact tatccgtatt gatgatcgaa
    20101 caaggtgccc ctggcggcca aatgaacaat acggcagaaa tcgaaaatta tcctggattc
    20161 gattcgatca tgggccccga tctagcttat aagatgtatg aaagtgtctc aagatttggg
    20221 acagaaaatg cttatggaat cgttcaagat atcaaggatc acggcagcca taaagaagtc
    20281 atctgtgaag ataaaagtta cgaaggaaaa acagtcatta tcgcaacagg ctgtatccac
    20341 cgtaaattag gcgtacctgg cgaggaagaa tttgctggac gaggcgtttc ttactgtgca
    20401 gtctgtgatg gtgcgttttt ccgtaacaaa cgtctagtag tcatcggggg tggggactca
    20461 gcagtagaag aagccatcta tttgacacaa tttgcttctg aagtagtgat cgtccaccgt
    20521 agagatgaat tacgtgcaca aaagatcatc caagaccgtg catttgcaaa tgaaaaaatt
    20581 tcctttatgt gggatacagt cgttgacgaa atcgttggaa acgaaatggt agtgacaggt
    20641 gtgaaagcac gtaatgtcaa aaccggcaat gaatttgatt tagcagcaga cggagtattc
    20701 atctatgttg gactggatcc attaactgca ccttttagag aagcaggttt gacgaacgaa
    20761 gctggctgga ttccaacaga caacgaaatg aaaacgaata tgccaggcgt ttttgccatc
    20821 ggagatgttc gtgaaaaaga tttgcgacaa atcactacag ctgttggtga cggcggtatt
    20881 gctggacaac aagtgtataa atacttggag tctttagaag aaacaacagc ataagtttaa
    20941 aaagagtgaa agaacggttt caattgttct tttactcttt tttttcgcgt gaaatccact
    21001 gaatccgatt tatgtgaata cttgcaatca tgcttgataa aagaatcctt tcttgattta
    21061 agcgtttttt ggtcctcata ctttcatatc taataagtga atgctatact tattaagaac
    21121 catatttcag aaagaggtgt tattttcatg tcttgggaac aagtgtatga acaatgggta
    21181 catgaagaaa atttagaaga aaacttaaaa aaacaattaa atgatctggg tgaagatcct
    21241 gaaaaacttg aagatgcttt ttatgcaccg ttggaattcg gtactgcagg catgcgggga
    21301 atcttaggac ctgggatcaa tcgaatgaac atctatacgg taagacaagc aacagaagga
    21361 ctagcacgtt tcatggatac acaaggtgaa gaaacaaaac gccgaggtgt cgctatcgct
    21421 tatgactcac gccatcagtc accagaattt gcaatggaag cagccaaaac actggcacat
    21481 cacaatatcc cgtcttatgt attcgaaagt ctgcgtccaa caccagagct ttcttttgca
    21541 gttcgtcatt tgaagacgtt tgcgggaatc atggtaaccg catctcacaa cccagcggct
    21601 tacaatggat ataaagtcta cggagaagat ggcggacaaa tgcctcctgc agacgcagat
    21661 gcattgacga aatatgtccg tgaagtcagc aatccgctaa aagtcgaagt attatctgac
    21721 gaagaagcta aacatagcgg gttgatcacg atcatcggag aagaagtgga cgctgcttat
    21781 ctagaagaaa tcaaagcagt gacgattgat cgtgaattgg ttgaaacaat ggggaaagat
    21841 ttgaaacttg tgtacacgcc acttcatgga acagggaaaa tgttgggcga acgtgcgttg
    21901 aaacaagctg gattcgaaca attcgtacta gttcccgaac aagctgtggc agatccagat
    21961 ttcactacgg tcaaatcgcc aaatccagaa gaacattcag cttttgaata tgctatacga
    22021 cttggagaaa aagagggagc tgacttgctg attgcaacag atcctgatgc agaccgttta
    22081 ggcgcagcgg ttcgtttgcc agatggttct tatgaagtat tgactgggaa ccaaatcgga
    22141 gcattgtttg ctcaatatat tttggaagca catcaatcaa gaggcacttt accagaaaac
    22201 gcagcggtat tgaaatcaat cgtttccagc gaattgccta cagcaattgc ggaacactac
    22261 ggtgctacta tgttcaatgt gttgacaggg tttaaattca ttgctgaaaa aatccaacaa
    22321 tttgaagaag accattcaca tacttttatg tttggattcg aagaaagtta tggttattta
    22381 gttaaacctt ttgttcgcga taaagacgcg atccaagcat tgcttttatt agcagaagta
    22441 gcagcttact ataaaaaaca aggcaaaacg ttgtatgacg gcttgcaaac gatttttgaa
    22501 acctatggct atttcgcaga aaaaacgatt tctgtgacaa tgagcggaca agaaggttct
    22561 gcgaaaatct ctgctttgat ggaaaaattc cgtaaagaag caccgacaga atttgccgga
    22621 gcaaaagtta ttcaaacaga agactttaaa gaattgacga gaacagaccg tgaaggaaac
    22681 gtagaaaaac taacaacacc gccatccgac gtattgaagt atacattgga agaaaatggc
    22741 tggatcgcta tccgcccttc aggaactgag ccgaaaatca agttttatat tggtgtaaaa
    22801 ggaagctcta cagaagacgc agccgaaaaa gtagcctctt atgaagcgat tatcaaaaag
    22861 atgacagaag aataaaaaac ttgagactga tggaaaaagt ggtaaacttt agaagtcagt
    22921 cttttttcaa gttggaggtg aatggattgc cagacacgca aaaatcaaaa gtagagaaag
    22981 tcagtcaatt attcaaaatg ttgagtgacc ccactcgtct taaaattctg ctatatctga
    23041 aagacgggga acaaaatgtg acagcaatca cccaagcagt ggaaatggag cagtctgctg
    23101 tatcgcatca actacgttta ttaagagaaa atcatgtcgt caagtctcat cgcgagggaa
    23161 aagcgatttt gtatagtctt gatgattctc atgtactgga tattttgaac cagacactca
    23221 aacatgtgga acatcaataa cgataggtgc
//
LOCUS       DAYYAJ010000036        22701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000036 DAYYAJ010000000
VERSION     DAYYAJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.036"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..3360
                     /locus_tag="ACHATZ_001196"
     CDS             82..3360
                     /locus_tag="ACHATZ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8403740.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3901..5808)
                     /locus_tag="ACHATZ_001197"
     CDS             complement(3901..5808)
                     /locus_tag="ACHATZ_001197"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8403741.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5938..6813
                     /locus_tag="ACHATZ_001198"
     CDS             5938..6813
                     /locus_tag="ACHATZ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8403742.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6893..7867
                     /locus_tag="ACHATZ_001199"
     CDS             6893..7867
                     /locus_tag="ACHATZ_001199"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8403743.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8047..9192
                     /locus_tag="ACHATZ_001200"
     CDS             8047..9192
                     /locus_tag="ACHATZ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8403744.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9329..9673
                     /locus_tag="ACHATZ_001201"
     CDS             9329..9673
                     /locus_tag="ACHATZ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8403745.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9944..11230
                     /gene="efmA"
                     /locus_tag="ACHATZ_001202"
     CDS             9944..11230
                     /gene="efmA"
                     /locus_tag="ACHATZ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8403746.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11368..11967)
                     /locus_tag="ACHATZ_001203"
     CDS             complement(11368..11967)
                     /locus_tag="ACHATZ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8403747.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12371..13492
                     /gene="mnmA"
                     /locus_tag="ACHATZ_001204"
     CDS             12371..13492
                     /gene="mnmA"
                     /locus_tag="ACHATZ_001204"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8403748.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13738..15159
                     /gene="arcD"
                     /locus_tag="ACHATZ_001205"
     CDS             13738..15159
                     /gene="arcD"
                     /locus_tag="ACHATZ_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8403749.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15173..16291
                     /locus_tag="ACHATZ_001206"
     CDS             15173..16291
                     /locus_tag="ACHATZ_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8403750.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16351..17643)
                     /locus_tag="ACHATZ_001207"
     CDS             complement(16351..17643)
                     /locus_tag="ACHATZ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8403751.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17793..18353
                     /locus_tag="ACHATZ_001208"
     CDS             17793..18353
                     /locus_tag="ACHATZ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8403752.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18482..18859
                     /locus_tag="ACHATZ_001209"
     CDS             18482..18859
                     /locus_tag="ACHATZ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8403753.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18792..20864
                     /locus_tag="ACHATZ_001210"
     CDS             18792..20864
                     /locus_tag="ACHATZ_001210"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8403754.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21075..22022)
                     /locus_tag="ACHATZ_001211"
     CDS             complement(21075..22022)
                     /locus_tag="ACHATZ_001211"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8403755.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22268..>22701
                     /locus_tag="ACHATZ_001212"
     CDS             22268..>22701
                     /locus_tag="ACHATZ_001212"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8403756.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7349 a         4143 c         4815 g         6394 t
ORIGIN      
        1 ttttgttttt tattgacagt aaaaataaat taggtaatac tttaagtaaa cgctttctaa
       61 tttgttacgg gaggaataca cttgttaaaa aaacgtataa aatacagtgc gctaatgatg
      121 aatctcctta tgctcagtac cccggtttta atttctgtcg ttgaagttgc tgctaacgaa
      181 caacagtctg tgaacgaaga aaatgcacac gtgaacattg aagggatgtc aacaatggag
      241 gaaataccaa gaactagtga agatatggat acggaagaag gaaatgatcc tatgatgaaa
      301 gaatcagaaa atgaaaagac aaaaaagcaa tctgaagaag caaatgaaga gtggaaaggg
      361 gttgttttcg gagaaagcac aagtgctagt aggtatgcca ttgaaccatt agaagacggc
      421 gtgcggctct cttccacgaa taacggcgga aaattccagt caagcggatc agatggaatg
      481 acctattatt atcaagcaat ccccaaagac atcaacttta ggatgcgggc gacgatcgaa
      541 atagaatctt ggacctatac gaatgcccaa gaaggatttg ctttaatggt aagagacgca
      601 gtaccaaaaa atcatttgtt tgggcaggca ttttattcaa attcatttgc catactaggc
      661 agtcggatcg aatatgtctg ggactcggat agacaagaag tggtcaacac gagcggcagc
      721 aaatatacga tgcgacttgg attggggaca cgggcaatca caggaatatc atctgaagat
      781 ccgcaagctg cgccagctcc tgggagtgtc tcagtggaaa caacaccttt agagacttca
      841 gcagggttct tggaaaaaga aacaggttct tataatatat tcggaaacgg tcatgggaat
      901 ctgtttccgg ccacgaactc gattactaaa ctggacgtag agatgaaaaa aacgaatact
      961 ggtttagagg catattatta cgatcctgaa acaggagaag aaatggcttc tgacatcatg
     1021 tatgactggg aaaaattata cgcttcagat gaatcggtta tttatgcagg atttgctgca
     1081 gcaaggaata tgacgatcaa ggtagaagga atcgaagttg cttactcaga tcctgcgaca
     1141 gatcctcctg ggcaagaaag acctactcgc ttgattgatc cgatttacac tgtgacctct
     1201 agtaatcatt caggaaacca agatcatact atgtccttta gagcaaatgc tgacggatta
     1261 ttgacaatta aaaataaagc aacaggagag gtgcttaaga acgcaaaaaa cttggctatc
     1321 actgcgaatc gggaatttga ttatcagaca aaactgatgg aaggaacgaa cgaatttact
     1381 ttcagtttta cgccagataa aaactatccg ccagaagaat acgtagagat gactagttat
     1441 gaaacaaaag agattcttca tacagtggac taccgtaaac ctaaatattc gacagtttat
     1501 gtaacaccag aagggtcaga tgcaggaaac ggaagcagac aaaatccatt atctctgaca
     1561 caagcattga gatacgcata tgctgggcag acaatcgtca tggcgcctgg aacttattcc
     1621 ttcgaaagag gattgacgat tccaaaaggc gttaacggga caagagaaaa cccgattcag
     1681 ttgattgcag agaaaaatcc agaaaacaag cgtcctgtac ttgattttaa aggaactgga
     1741 aatgggatga cactgttcag tcactactgg ggtttacgag ggtttgacat tacaaatagt
     1801 gcagctatgc aaaaaggatt acagatttct ggaaatcata atttgatcga agagattcat
     1861 gcttatcgga atggaaatac aggaatccaa atcagcggta gctcaaatga tcctttcgaa
     1921 gcatggcccg cgcataactt agtcaagaac tgtacatctt ttgaaaatgc cgatccagga
     1981 tttgaagatg ccgatggatt tgcggccaaa ctgacaatag gagaaggaaa tgtatttgac
     2041 ggttgtatcg cgtatcataa tgcagatgac ggttgggacc tttttgctaa aaatgaaaca
     2101 ggttcgatcg gaaaagtaat catcaaaaac tcagttgctt atcgaaacgg ctgggtacca
     2161 ggaattgagg gagaaggtaa cggtaatgga ttcaaaatgg gcggttcttc tttaacgggc
     2221 cctcatgaat tgatcaatag tttgtctttc gaaaatcttg cgaaaggaat cgattccaat
     2281 agcggaacag atattatcgt caatagcagt acaagtttta ataacggttc atataatgtg
     2341 gctctttata cctcttcagc aggaaacaca gactaccatg cttctggtat cttatctttc
     2401 cgaacagaga atcttgagat gagagagcag atccgcccat tgaatcaaaa cgaagatcaa
     2461 atctaccact cgttgaacta ctattggaat gaacgagaga aacaatcaga aaatactctt
     2521 ggacatacca tatcaaaaga ttggatcgag tctctaaata catgttcgaa agaaggtcag
     2581 cagcctgtta cgcgctatga aaatggaagt atccatgttc atggactgat gcagattaaa
     2641 ccggggaatc gtcaaacaga aaaggaaaga gtggatggtc ttccattatc tgtaggtgct
     2701 atttttgacg gagaaacctc accgtcttcg gaatatccag agtatgcaat cgtacaagaa
     2761 ccaaatcaga caaaaccaaa tgaacaaaat aatccgatta ttatacaaga agcaaatggt
     2821 gtggcggaga atgcagaagg aaatatcatt gaactgaagc cttttacagt ttttgtgtca
     2881 actttcacac caaatcaagg aacgcctgtc tttgaagata tgcgttattt cccagaaaag
     2941 atccaaatcg ttgaaacaca gtcaagtgct caattttcta atcataccgc tattttttca
     3001 gcaccttcaa aaggagaata tacgctagaa gtcacttatc gttcggaaat atttgatggg
     3061 gaaaaatggt ctgctcatac agaatctgtt acccagcaaa aagcgatcac tgtgcgagaa
     3121 actgccgatg tgcccccggg aaatgacaaa ccagaaagag aaaatccagg aaatgaatca
     3181 ccaaatggaa aaccaaatgg aaaaccttct catccggata ggacagcaaa agaaagtgaa
     3241 aaaaatggca ctcaaggact cccaaaaacg ggagaattta ataacccgtt gctagcatta
     3301 gctggaggaa ttttgttaat cggtgtagtt gtttatgtca tgaaacagcg aaaaaaataa
     3361 gtggaatttc tccttttatg atgtaaaaca attgactgag gaaagtaaaa atagaaggaa
     3421 atgagaccaa tagatgtctc acttccttct atctttttat agatgaggtt ctccttgctg
     3481 cttttgattg gctgttctta ttacttgatg caggacagct ttttcacaat ctctccacta
     3541 aacggtgttc aatcagctga ccctcggtat tagctcaaca aacggcaaaa tatgaggaac
     3601 tatttttccg tttttttctc taatactcag gtcaggacgc tgatttcaca acctctcgat
     3661 aaacggtgtt caaaagccag tttctcggta gtaagtcaaa tttatgcaaa aatgtggaaa
     3721 gccattttca caaaattttt cttactactt gaaactaagc agctttttca caacctctaa
     3781 tacatggtat tccagaagta acttcttcga aaaataagcc gaaatgtcac aaaaatttga
     3841 gaagcgattt tcccaaattc cttcttattt cttgaagctg accacttctg gcacaacctc
     3901 ttaccagatt cgcactcttt tttctggcgc tagatacata gcgtcttgtt ccgtgatacc
     3961 aaatgtttca tagaattcgt caaggttttt tggttgcacg tttgctcgga gtttagcagg
     4021 ggcatggaca tcgatttgca gtaaaagttg ttggtattct ttttttgctt ttgtacgcca
     4081 aatcgttgcc caattgacaa agaattcctc gatagaaaga tctgattccg tttttgctgc
     4141 ttccaatgca cagcttagac cgcctgcatc agcaatattt tccgaaacgg ttaattttcc
     4201 gtttactttt ccgtcagcaa aaggcagacc atcgaattct tcgatcattg cttgtgcttt
     4261 gttttgaaat tgtgtaagat cttcttctgt ccaccagttg ttcaagttgc catattcgtc
     4321 aaataaagcg ccgttattat caaatgcatg ggaaatctca tgagcgataa cagctccgat
     4381 tccaccataa ttagcagagc ttgattgttc tagactgtaa aatggtgctt gaaggatcgc
     4441 tgccggaaag acgatcacat tccgaaatgg atgataataa gcattgaccg tgtcagcact
     4501 catttcccat tctgaacgat ccactggttt gttccatttg ctgaaacgat cttttaatgt
     4561 gagtttgctg aaaacaagag tatttgaaag caaagtacca ccctgatctg caggtactgt
     4621 tttgaactgg tcgaataatg ctgggatctt atcggggtag cctacttgga tacctaagtt
     4681 atttaatttt atgacggctt tttcttttgt tgcatcccca agccagtcat tttcttctag
     4741 acgtttttta taaacggcga tcattttttt caccatttgt tctacgtctt ttttagcagc
     4801 ttcgccaaaa tatttttttc cgtagtaatc gccaactact tgatcgaatt gtccagaggc
     4861 aaggtaatac gctgcttttt tctgaggcat tgcttcgtct gttccagaca atacgcgaga
     4921 atagattccg ctgacttggc ggaattcttc tgatagataa ccactaagag aacgtactgt
     4981 tttaatgatc atccagcttt tgaataacgg aaaatgagct tctgtcagga tgtcattcaa
     5041 atgatcgaag tacactggat cagttacgat cactttatct ggagttgtcc caatcaaacc
     5101 cgtgacaagt tttttcaagt ccagtttatc tgtgtattta ataaatgtat caaaagattg
     5161 cggattatac attttgctgt aatctgcgtt ttcttctgca ctttttacat gaggagcaat
     5221 caatttatca aattgcactg cttgttccgt gattgcttct gcctgacttt tttctttgcc
     5281 ggcaagctct aataactgaa cagccatatc aaagaaaacg cctattagtt gtgcaccgtt
     5341 gggatggtct gatgcataat acgtcttatc tggaaggaat aaagaaggcg gataagcaaa
     5401 gaaggcgttc gtttgcgcat tcttcatatc tgcatctaca tccagattaa atggcaatgg
     5461 taatgtatcc aacagccatt ctggaaattg cttattcaat tcgtcgaaag actgcaatga
     5521 ttcaactttt tcgattatag gttgaagcgg ttgagcatgc aactcatttc ttcggtcata
     5581 atcgtttgcc agttgataat atgcaaggaa atgtttcatt ttttctgaag acactttttc
     5641 tggatgggca agcagatcgt ctgtttctcc catcaaaagt tggtcgatcc cgtcaactaa
     5701 atcttggaaa ccgcctgtag ctggtttatc tgcagggatt tttgctgttt tttgccattc
     5761 ttcattgact gcttcataga aatcttgttt caatcgttct ttgttcattc atcgatcgct
     5821 ccttttttgt ttcgtgtgcc cattatctta caaaatgaag gaattaacaa atagaacata
     5881 aaaaattgaa aattcgaaca atcaaactga tttgtttttt tcaatccttc acttataatg
     5941 aaaaaaagga ggcgatccca actgaatcaa gtagtggata aacctttata tttcaaagta
     6001 gataaaaaaa ttaaaaaatt agccagcact cagcagctgc agtcaagaaa aagtaaattg
     6061 ctttttttag ctctggtttt tgaagatcaa agttatgtga ttatcgatca atcaggacat
     6121 ccggtagaat attctccagc aaaatatact tatcaagagg gtctttcttg taaaaaatgg
     6181 aagctgatca atgaagagcc aatcgagtta tctagatgga tcaacagaaa agaagatatt
     6241 cccgttttga tcgaagaaaa aaggtcagga aaggaactcg caaattgctg ggtgggattg
     6301 ccagaagaac gttttttgag gtataagaaa tgggctaccc cctcaggcta tctttgtggt
     6361 acttacgcgg cagcagttct ccttgcctat tatcaagact atcgtaaggg gtggatgctt
     6421 ccaaatgaga ttcgcaagaa aaatacggct gattcgttgg ttttgacgaa ggcattgcgt
     6481 agccagatcc aaccactagg acttcctacg attccttttc aggttagtac agggatttct
     6541 agttttttaa agaagaatgg caatcatgag cgtgcacgag caactcttct aggttcatgg
     6601 cagcgagcta caaaacgtat acgtgaaggc aaacctgtga tgattggtat tttgaaagta
     6661 cttggcagta catatggcaa tcattgggtg acagcgtacg cttattttga aactgaaaca
     6721 ggagaacgtt attacaaagt acacgacaac tggggcgatt atcataaagt cattcctgcc
     6781 agttggagta acggaacagt ttcacttcct taagaatttg ctgaaattaa cagaaaatct
     6841 tggaaaaata tgttaaacta tcgaagaata gagaagtttt ggggaggact acatgagtaa
     6901 taagtatcag gatgacacgt tgaagatttt tagtttgaat ggcaaccgtc cattagctga
     6961 aaaaatcgcg aaagtgttcg gaacagaatt aggtaaaagc gtcgtgaagc aatttagtga
     7021 tggcgagatt tccatcaata tcgaagaaag tattcgaggg gatcatgtgt atatcgttca
     7081 atcaacgaat gcacctgtca atgattacta tatggaacta ctgatcatga tcgatgcaat
     7141 gaagcgagca agtgcgaaaa cgatcaatgt cgtattgcct tactatgggt atgctaggca
     7201 agaccgcacg gcgaaacctc atgaaccgat cactgcgaaa ttgatcgcta acttgatcga
     7261 agaagcaggg gcaactcgag tactgacttt agatctgcac acggttcaag tacaaggatt
     7321 ctttgatatt cctgtagata atttatttac gatgccgttg tttgctcatt attaccgtca
     7381 attaggtctg acgggagatg acatcgtagt tgtttcgcca aaaaacagcg gtgttcagcg
     7441 tgcacgtagt ctttcagaat atttgaacag cacattagcc atcgtagatc atgcagatga
     7501 tgcttcaact gagggtggtt atgtgatcgg tgatgttcaa ggaaagacat gtatcatggt
     7561 agatgatatc ttgaacacag gagcaacgct tgcacgagca gcaaatgtat taaaagaaaa
     7621 cggcgcaaaa gaagtatatg cctgtgcgtc acatggatta ttgtccgatc atgcgaaaag
     7681 tattttagac aatgcaccaa tcaaagatat ttgtatcact gattctgttt tcacagaaga
     7741 aaatcgtcat ccagaaaact taacaatcgt tacatgttca gaattaatgg gtgaagcggt
     7801 caaacgcatc catgaaaata caccgatgag tccgttattc cgtctggaag aaaaagaatt
     7861 cgaataaaca aaaaaaagag ggaaagaagt tgtgacagaa gtgagcagct tcaaggagct
     7921 gcgacaagac ttttgtcaca gctccttttt tctatgccca tgaaacgtcc tctagacaaa
     7981 aatgagctct tctttggttt gttcgctatt ttatgctaga atgtgcctag caataatttg
     8041 gaagggatga atgaagtgaa aactatttat ctggatcatg cggcaactac ccccatgcat
     8101 ccacaagtaa ttgaagcgat gacggcaatc atgcaagaca cgtttggaaa tccttcaagc
     8161 atacatggat ttggtcgcca cgcgcatgaa aaattggaaa cagcaagaca aatcattgca
     8221 gacagtctgc aagcaaaacc tcacgagatc atctttaaca gtgggggaac agaaggggat
     8281 aatacagcta tcttagaaac agcgttctcg cgtcaaaaag aaggcagaca tctaatcact
     8341 acagctatcg aacatcctgc tgttcttaat accatgaaat atttagagac aaaggggttt
     8401 gacgttacat atctgcctgt agatgaaaaa gggaatctgt cgatcgaaga ggtcaaaaaa
     8461 gcgttacgag aagatacgat cttggtctct gtgatgtacg ggaacaatga aatcggaaat
     8521 ttgatgccaa tcaaagaaat cggtgaactt ttagcggatc atccagctta tttccataca
     8581 gatgcggtac aagcgtacgg aaaccaaacg atccatccag aagaattagg aatcgattta
     8641 ttaagtatct ctgctcataa aatcaatggg ccaaaaggcg tcggtttctt atataaacga
     8701 gacggagtaa atatccctgc attgctttta ggcggggagc aggaagaaaa gcggcgtgca
     8761 ggtacagaaa atttagcagg gatctggggt atggcaaaag cagttgaact gctgtcacct
     8821 gaagaacgtg aagcacgtaa caacaaatat aaaggattcc agcaattgat tcttgataca
     8881 ttagaagcaa acgaaatccc ttatcaagta aatggtgatt taacgaataa actccctcat
     8941 gtgttgaatc ttcgcttaat tggcgaagca aatgatttgc tactgatgaa gcttgatctt
     9001 aatggggtcg ctatttctac tggttcagct tgtacagcag gaaatatcga gccttcacac
     9061 gtattagaag caatgtatgg agaagataca ccgatactaa aagaatccat ccgtatcagc
     9121 tttggtttgg gaaataccga agacgacatc cagtatttcc ttgatcaatt agttcgtgct
     9181 gtcaaaaaat aactagtgaa gtcattctta tatgaacaga tgtctgagag aacgccttca
     9241 ttttttaggg aacttaactg aaaaacctag taaaaatcag gataaacttt tataataaag
     9301 agaaactaat tttatcgagg tgaaaatcat ggcatttgaa acaacagcaa cggtactggg
     9361 cgcaccaatt agctatcgtt tgcatcctga tgcaaaaaga tatacattgc gcgataatgg
     9421 atttacagaa acaaacggag gcaattatca attgatccga ccattagatg ccacgccgca
     9481 aagcaaagaa ggattcaaat taaaaatcac tgtatcaaaa gatattcaaa cattgaaaat
     9541 gtcgatcact acagcaaatg gattgaaagc agtcaatatt ttcaaagatc caaaacagaa
     9601 aatgagccaa gataaatttt attttctgat ggatgggatg attagccgtg ggttattcga
     9661 aaaagtcgaa tgatctgtgg atataaaaag acaactagtt ttgaaacggc ctattgttag
     9721 atgactaact ttgtaaggac gacgcaaatt agttgtcttt tttattttta aatttaacac
     9781 atgttgttat ttttaatatt ttgctgattt attgtttcta agtaaagaaa tttaaattga
     9841 aacgattgtt atttagggaa aaaggtattt tttgaaaaaa gaatcccgta taatgattgt
     9901 tgtgcaaacg ctatacaaaa tgaaaaagaa aagagggatg tttatggaaa atgaacagtc
     9961 ggtcgtttta acgaattgga agagaaatta tttgttcttt ttatcgggac agtttttgtc
    10021 aggaatcact agtatggtcg tccaatatgc gatcatctgg tatttgacaa gagaaaccgg
    10081 ttcggcgaca attttgagtt ttgctacttt actaggaatg attcccatgg ttttactaag
    10141 cccatttgta ggaccgctag tggatcgctg ggataaaaaa gctcttttga ttgtcacgga
    10201 tattattgta gcgattttcg cgcttatttt agcagtagtc ggaacgattt cagaatcctt
    10261 cccgatttgg ctagtttttg tgtctttgtt tatgcgttcg gtcgcacaaa cattccaaat
    10321 gccaacgatc cagtcgatta tgccgacaat cgttccatct tctcacataa caaggacgaa
    10381 tggacagtta ggaatggtcc aatcagcgaa tttcatcatt gcgccagctc taggtgcggc
    10441 tttgttttca gtcgttcctg tcaattacct tattttatta gatgtattgg gggcagtttt
    10501 tggtgtcggc ttattgattt ttgtgaagat cccaaaagtt tctcctgaaa tattggaagt
    10561 tccgcttact atttttaaag atgcaaaatt tgggctgcag caattgatgg ataataaagg
    10621 gttatggtac ataacgatca atggtgcatt tgtgatgctg ttgttcatgc cagcaatcag
    10681 tctgtatcca ttgatgacgc tagattactt tggtggttcc gttggacagg cgggagcagt
    10741 ggaagtagtc tacgcagtcg gaatgctctt aggcggtgca ctgatcagtt ttataggaac
    10801 atggaaagac cgaatgaaac ctattattat agcctacatc attatgggcc tgacgatcgg
    10861 tgcaagcgga ttggttccaa acgatagtca agggttcttg tactttctta tcttgaatgc
    10921 aggagcaggt tgtgcaacgc catatttcaa tacactgctg atggcaatga tccagcagag
    10981 ttatgaatcg aatgttttag gtcgtgtctt agggaacttc aactctttga tgaacctggc
    11041 aggtccaatt ggattattat ttgcaggacc attagctgat cgtttagggg tagaaaaaat
    11101 gttcttgttt tccggaatcg ggattttatt atgcggaatc gttttatttt taacgtcagc
    11161 tgcccgaaat tatgataaag aattacagaa aaaactggta aaagaacacc atgagcaaaa
    11221 agacgaatag tatggaatgg agttgtgaca gaagtcttgt tacaactcca tttctgatcc
    11281 taatgttcct gtggcaataa gcctactcaa atgaaaaata tgagtaatta tctttatttg
    11341 tttaatactt gatatagggc agttttttca cgacctatcg tacttcctct ttgcttcata
    11401 gatttctttg atttgttgct cagataacgg agtgccaaat ggtagttttt cctttaagag
    11461 ttctctttcc ggtgcgtcta cgcctacatt gacatcgttc tcgataggta cgctgtaatg
    11521 atggatatgt ccatgaagat tgcggatagt gtcgttaggc ccgagcaaca tcggataatg
    11581 agtcaagtaa tattgttgat ggtcgaattt aacgatggca cctacatcat aaaaataaaa
    11641 tttgggactt ccgtcagtaa gcctaggatc atttttttct aaataacgga aaaaagcgcg
    11701 actgtcatgg ttccctttga tcaagtggat tgttccttgc agttgagtga ccagttccaa
    11761 aatttctgca tttgcttttt catattgcgg atgcatagca aggtcgccta gatgataaac
    11821 atggtcggtt tcttttatta ctgcattcca gctagtgatc aactgttggt tcatttcatc
    11881 cacatttgca aagagccgcg gagcaaagtc gttttttcct aataagcgtt catggaaaaa
    11941 gtgcatatca ctgataaagt aattcatgta tcttttgcct cccttttttc ttagagtaaa
    12001 caaaatcact tatctgagca actcataaca tctaaaaaga aaaggttgta aatgagacgt
    12061 gcagctttaa gcgataagaa gcaattttga aaaatagttt ttcacaattt ataaaattat
    12121 tccttattgc cgaaaggctg gcccttttta tgagtatgcc aattttttat atcgaatgct
    12181 caaaagatga gtgatttttt ggttgactat catgtttgtg tactgaatag gtccagtaga
    12241 acagtatttt tacgacttga taattaaggg atgaaatttt cagaaatatg acgtataatg
    12301 taaaatactg aaaagttgcg accttcaact cgtagatgtt tcagaatcta attgaaatga
    12361 tggtgatatc atgacagaca acagcaaaac acgcgttgtc gtagggatga gcggcggtgt
    12421 cgactcatct gtaacagcac ttttgttaaa ggaacaagga tacgacgtga tcggaatatt
    12481 catgaaaaat tgggatgaca cggacgaaaa cggtgtatgc acggccacgg aagattataa
    12541 ggatgtagcg aaagtagctg ctcaaatcgg gattccttat tactctgtga attttgaaaa
    12601 agaatattgg gatcgtgtat ttgaatattt cctagccgaa taccgcgcag gacgtacacc
    12661 taacccagac gtgatgtgca acaaagaaat caaattcaaa gcattcttgg attatgcaat
    12721 ggatcttggc gccgattatg tagctactgg acattatgcg caagtcactc gcgatgaaaa
    12781 cggcgtggtc cacatgcttc gtggagtaga caataataaa gatcaaactt acttccttag
    12841 ccaattatca caagaacagc tttcaaaaac gatgttccct ctaggcggca tggaaaaatc
    12901 agaagtacgg gcaattgctg aacgcgcagg ccttgcgact gctaagaaaa aagattcaac
    12961 gggaatctgc tttattggtg aaaagaactt caaacaattt ttgagcaact atctgccagc
    13021 aaaaaaagga aatatggtca ctcttgatgg tgaagtcaaa ggacaacatg ctggcttgat
    13081 gtattatacg attggtcaac gtcaaggact agggatcggt ggcggaggag attcgcaaga
    13141 accctggttt gttgtaggaa aagatctagc gacaaacaca ttatatgtgg ggcaagggtt
    13201 ccatcatcct gcattgtatg ccacaagctt ggatgcaagt gaaatccact ttacgacgaa
    13261 tgaaccaatg ccaaaagaat tcaagtgtac agcgaaattc cgttatcgtc agcaagacgt
    13321 tcctgtgaca gttcgcttat tggatgataa tcgtgcagaa gtcgtattcg acgaacctgt
    13381 acgtgcaatc actcctggac aagcagttgt attttatgat ggaatggaat gtctgggtgg
    13441 tggtttgatc gaccacgcat atcaagaaac aaaagtttta caatacgttt aagcgatatt
    13501 aaatgaatca aaacagctat ggctaacaaa agaaacacct tttcttttgg atagcatagc
    13561 tgtttttttg caataaaatt aatttttatt cattttatac ttgaaatact tgtgtaaaaa
    13621 gttcataata tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa
    13681 atagaaggga atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg
    13741 gaaaagaaat cgacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca
    13801 atcggaagcg gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca
    13861 gcgatccttt catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat
    13921 catttaggag aaaagcgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca
    13981 tttggtaaat taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga
    14041 aatgttgctt ttgcaacgat gctgatgagc gccatgggag aatttttccc gatattcaaa
    14101 ggtggacaaa atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta
    14161 ttagtaaaca atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa
    14221 ttagtgccat tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc
    14281 actgctgatt tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga
    14341 acgatctggt tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc
    14401 gaaggcgcaa gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg
    14461 atcattggtc tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc
    14521 gtcttatctc aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa
    14581 ggaatcgtgg gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt
    14641 ggttcttggt tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac
    14701 ggtctgttgc cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta
    14761 gttgttactg caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc
    14821 tatcaatttg cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg
    14881 atctatcaag tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg
    14941 attgctgtag tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta
    15001 tgtagtattg cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa
    15061 catcaaatta cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt
    15121 tcaatcgggt tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat
    15181 cgcaaatttc ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat
    15241 tgcacaaagc tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag
    15301 cgtaagtaaa tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg
    15361 atcagatgtt ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat
    15421 cttacaaacg aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc
    15481 aggagaccat gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc
    15541 tttaggtgcc gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt
    15601 agaacagcta gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa
    15661 caatcctaca ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg
    15721 tacagtagat gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga
    15781 attcttgtcg atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac
    15841 ctactccatt ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt
    15901 ttttcgaaag tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc
    15961 tgtacatgca ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga
    16021 gaaccttgtg attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc
    16081 caattacgtc tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg
    16141 tatcaattta ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc
    16201 gaagcatgcc cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct
    16261 cttatctgag ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac
    16321 aactgttcaa gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga
    16381 agctatggag actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt
    16441 attgtgtcgt acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc
    16501 gcgataacca aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt
    16561 tgcttggaaa gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt
    16621 tgtgatcaat gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg
    16681 taaggcagct aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc
    16741 aaaacccaaa ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa
    16801 tcttggagcg gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa
    16861 cgtaacattc ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat
    16921 ggaattgttg gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg
    16981 atatttcaag tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac
    17041 aaatttttca tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt
    17101 aatgaaaaag gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc
    17161 aagtaatgtt cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc
    17221 ttttggcgtc ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc
    17281 ttgaagcaga cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat
    17341 gacgattgcc attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc
    17401 taattttccc caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa
    17461 tccgctccaa aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga
    17521 cataaaaggc atgactaaac taaatccgat accagccata aatgttccaa accataagac
    17581 aaacaagttt tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc
    17641 caattagact ccttctttct gaaaattttc atctacagct ctttattata aactacaata
    17701 tgtagtttat caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt
    17761 gaaggattta ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga
    17821 aaatcatcga ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc
    17881 agcatttagc caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata
    17941 ttcgtgatgt caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc
    18001 gacgggctct tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc
    18061 gatcctttgc tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca
    18121 atgaagctgt tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg
    18181 aggcttttca attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga
    18241 tccacggtta tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc
    18301 cagaagacag ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca
    18361 gattcatttg taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct
    18421 tgcaactttc ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag
    18481 tatgtatcaa gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg
    18541 ggaagaagat attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta
    18601 cgaaaaacaa tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt
    18661 tctcagcgcc ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt
    18721 acagcaatac aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag
    18781 aaaggaattt tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa
    18841 gcaaggtgct tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg
    18901 ttacttgggc ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat
    18961 ggcagagctg gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac
    19021 ttcctaaaac aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct
    19081 cagaagcagt cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct
    19141 tagagtatgt gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata
    19201 aagcaatcaa aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg
    19261 aatctgctct tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct
    19321 taactgtgaa gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat
    19381 tagtgagaga ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca
    19441 cgttgcaagc tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa
    19501 cacaagaaga agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag
    19561 aagtccaaga aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc
    19621 aaagagaagc caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa
    19681 gtttgtatga gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa
    19741 atgacatgaa aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg
    19801 tcaagcagta tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta
    19861 aagtgaccga tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa
    19921 gtaatgaaga gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc
    19981 aaccccataa agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt
    20041 ttcggcaaaa tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa
    20101 cagtcaaatg ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat
    20161 ctgcaccgcc taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc
    20221 tgggaacccc agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg
    20281 aacgtacccc tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa
    20341 atccctcttt agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg
    20401 ctgcaggaaa gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct
    20461 tggtgaatga gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga
    20521 aatgcccaga atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg
    20581 tctgcaccaa ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc
    20641 gatgcccgca atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc
    20701 gttgtaaata cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa
    20761 tgaccaaaca tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag
    20821 aaagcccatt agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg
    20881 ttgtgataga agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa
    20941 atttttgtga aatttcggct tatttccgaa gaagctactt ctggaacacc atttattcgg
    21001 aaaaaaggac tgtgacaaga cttttgtcac agtccttttt catttaaaaa aatgtttact
    21061 tcatttgacg tatattattg gtgaggcttc aattcatttg gcttcacttt ttctgtttct
    21121 cttatgatat aaacaggacg ctgtttcgtt tctaagaaga ttttcccaat atatttcccg
    21181 atgattccaa gacataacaa ttggattcca ccaatgaaca ggaagatcga gaccatggaa
    21241 ggccatcctg atgtaggatc accaaacaac aatgcccgca aaataatgaa caaaattgca
    21301 ataccagaac cgacacaaga caaagcgccg ataaatgaag caatcgttaa cggtgcatcc
    21361 gagaagttca cgataccatc aatcgagtag ttgaataatt tccagaagga ccaagatgtt
    21421 tcgccagcca cacgttcgcg gttttcataa gaaatatatt ctgttttaaa tccgacccag
    21481 ctaaataatc cttttgaaaa tcggttgtat tctgttaatt ctaggacggc atccaccatt
    21541 tgacgagtca tcaagcggaa atcacgagcg ccatcgacca tttctgtatc gctgatttta
    21601 ttgatcaact ggtagaattt tttcgcaaag aaactgcgta tcgcaggctc gccttcacga
    21661 gtcgaacgcc gggtgccgac acaatcgata tcagaagtag tgatccggtt gatcatctca
    21721 ggaagcagtt cgggcggatc ttgcagatca acatccatca cggtcactaa atctcctgta
    21781 gaagcttgca atccagcgta cagagccgct tctttcccaa aatttctgga aaatgagaca
    21841 aagcggacat aaggataagc tgaggctagc ttcttgattg ctgctaacgt accgtctttt
    21901 gatccgtcgt caataaacaa atattcgaac tcatgggcac ctttgaattt ttcaacctca
    21961 tcaaaaaaaa gaggaacagt tttttcttca ttaaaacatg ggacaacaat tgatactttc
    22021 atcgtttcta tcctttcaat cgtatgttta tatatggttt acattcaaaa tttaataaac
    22081 cttaaataac acctttaaaa tttgatttta cgtaaaagaa caggtattct ttttgtaaac
    22141 taacgtattt agtttaccat gtttcctcgg tttatcaaac ttttaagaac aatttttatt
    22201 agttggcaag tagttttgga tagagaaaaa ggaggaagtt ccagtctatt gactaggagc
    22261 aaacaaaatg aaaatacaca cacctatcgg ctttttatgg ttagatgcag atgaatccgg
    22321 attgactgct gtatcttttc agccaatttc agaaagcgaa tcagcgaatc agcgaatttt
    22381 aacgcgcaca caagaacagt ttgaagaata ttttgcggga aaaagaaaag aatttgatgt
    22441 tcctttatct ataaagagag gtacgccttt tcagcaaaat gtctggaaag ctttgtcgac
    22501 cattccttat ggagaaatcc gaacgtataa agaagtagcc gttgctgttg atagtccgaa
    22561 agctgtgcga gcgattgggc aggcaaatcg aaccaatcca ttgccaatcg ttattccttg
    22621 tcatcgagtg atcgggcaaa atggaaaact gacaggatat atgggaaacg caatagatgg
    22681 ggttgaaatc aaacgccaat t
//
LOCUS       DAYYAJ010000037        22269 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000037 DAYYAJ010000000
VERSION     DAYYAJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.037"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..543)
                     /locus_tag="ACHATZ_001213"
     CDS             complement(1..543)
                     /locus_tag="ACHATZ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8403757.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(564..1655)
                     /locus_tag="ACHATZ_001214"
     CDS             complement(564..1655)
                     /locus_tag="ACHATZ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8403758.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(1663..3873)
                     /locus_tag="ACHATZ_001215"
     CDS             complement(1663..3873)
                     /locus_tag="ACHATZ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8403759.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(3889..6417)
                     /locus_tag="ACHATZ_001216"
     CDS             complement(3889..6417)
                     /locus_tag="ACHATZ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8403760.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(6469..6879)
                     /locus_tag="ACHATZ_001217"
     CDS             complement(6469..6879)
                     /locus_tag="ACHATZ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8403761.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(6892..7110)
                     /locus_tag="ACHATZ_001218"
     CDS             complement(6892..7110)
                     /locus_tag="ACHATZ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8403762.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7107..8099)
                     /locus_tag="ACHATZ_001219"
     CDS             complement(7107..8099)
                     /locus_tag="ACHATZ_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8403763.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8178..9089)
                     /locus_tag="ACHATZ_001220"
     CDS             complement(8178..9089)
                     /locus_tag="ACHATZ_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8403764.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9108..9452)
                     /locus_tag="ACHATZ_001221"
     CDS             complement(9108..9452)
                     /locus_tag="ACHATZ_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403765.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(9472..9750)
                     /locus_tag="ACHATZ_001222"
     CDS             complement(9472..9750)
                     /locus_tag="ACHATZ_001222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403766.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10109..10609)
                     /locus_tag="ACHATZ_001223"
     CDS             complement(10109..10609)
                     /locus_tag="ACHATZ_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403767.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(10610..11155)
                     /locus_tag="ACHATZ_001224"
     CDS             complement(10610..11155)
                     /locus_tag="ACHATZ_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403768.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11152..12408)
                     /locus_tag="ACHATZ_001225"
     CDS             complement(11152..12408)
                     /locus_tag="ACHATZ_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8403769.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(12768..13079)
                     /locus_tag="ACHATZ_001226"
     CDS             complement(12768..13079)
                     /locus_tag="ACHATZ_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403770.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13102..14577)
                     /locus_tag="ACHATZ_001227"
     CDS             complement(13102..14577)
                     /locus_tag="ACHATZ_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8403771.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15026..15478)
                     /locus_tag="ACHATZ_001228"
     CDS             complement(15026..15478)
                     /locus_tag="ACHATZ_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403772.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(15480..15845)
                     /locus_tag="ACHATZ_001229"
     CDS             complement(15480..15845)
                     /locus_tag="ACHATZ_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403773.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(15944..20227)
                     /locus_tag="ACHATZ_001230"
     CDS             complement(15944..20227)
                     /locus_tag="ACHATZ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8403774.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(20374..20613)
                     /locus_tag="ACHATZ_001231"
     CDS             complement(20374..20613)
                     /locus_tag="ACHATZ_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403775.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(20703..20945)
                     /locus_tag="ACHATZ_001232"
                     /pseudo
     CDS             complement(20703..20945)
                     /locus_tag="ACHATZ_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21058..21294)
                     /locus_tag="ACHATZ_001233"
     CDS             complement(21058..21294)
                     /locus_tag="ACHATZ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403776.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6530 a         4832 c         3313 g         7594 t
ORIGIN      
        1 ttaatacgca ctccgtacat aataaatctt gatcgcatca atcttttgag cttcttgacc
       61 tactttcaat tgaagtaata caaattgagt aaccttattc attgtttctt ctccaaataa
      121 caaatattga tccggattct ctatatcttg tgtcacctgg taaagtttcc cagttgtttt
      181 catttgtaca tgcgggtcta catcaatact attgtcttta atacaacgat ctgtcatata
      241 gtccctcaca gcttgattgc gtttataaac atctgtgaaa ttactccatt ttctagcaaa
      301 atcttttaag aaattttttg tcatttcatc tatttgcatt tgttgcgctg tctcgtcttc
      361 taatgaacca atttgactgt tactttcagt actttgaaga gttccacttg tttcttttac
      421 tgcttcgatt gtttcttctg tcgttctcaa ttgtgttttt tgtttttcag aagaaaacca
      481 atgacaacca gtaagaccta tcaaacataa ccaactaaat actaaaatta tccctttttt
      541 cacgtttcta gtcactgcct ttcttatttc accttacgaa ccagactacg aatttttgtt
      601 tgattgtacg tgcgttgata ccgagccata atccgtcctt gctcggcatt ctgttccaca
      661 ctagagaaat gttgtccgcc atatgaaaca tctgttataa ttccggtgtg accatagatt
      721 ccaggagaca atgcaccacc tgcttgccaa ttcaccacat ctcctgcttg taaatcactt
      781 ggttttgggt tcatgattac ttgccaacca taggttgtcc agttataatc ttcccctatt
      841 aacatcgcaa acatgtgtcc agaacccatt aactgtgggc cacctaattg agatacatac
      901 catgccgtac caccataaca ttccccatta tttacggatc gtccaatgat ccgatccaaa
      961 accttcgcat tgcctttact ttttcctgca ataagcgaag ttttttttgt tggtaggcta
     1021 ttccaatgct tgatcgcaaa agtatcatat tccgttaagt tataggacgt tatgatactc
     1081 attgctcctc gataatacgg actcccttcg gtaatacctg ctgccgcata gcctccacga
     1141 cctagaatca ataaacaact tccaggatct ttattgccca cacattgatt aaagttgctt
     1201 tggctcgcca ccataaagcc ataatagtca taacaagctt ttaagttcgg aaacttcata
     1261 tagaaaccac cttcagaacc accaattcca cgtgcagaac cttgtgggta gggacaacca
     1321 ggaaaccagg taatcccaaa aaagttcgta tcgtttcttc cagattgtga ctgtccataa
     1381 gctgattcat agcctaattg agaaagaatc acactcggta ataactgata ctttacagct
     1441 gcaccaactg ctgcttgcgc atgttcatct gtgattggac cacgtccttt aatctctgaa
     1501 aaaatcggta atccttcggt atattcccca ttaaaagcga cagctagacc tgtagcttct
     1561 cctccatgct cagaaagttc tcctgaaaaa aagaatggca atagaaaaaa gggaagaata
     1621 aagaaaagaa gacaacctag acaccctttc ttcatttctt cctcatcctt tcttcgataa
     1681 tggtttttgg actgttcgtc cttttggtaa agtaaaatca gttcctggtt ttgcgtccaa
     1741 aatcggcagt gttttaggat ccttttctat tttttccgat tgctctgttg gcttatctaa
     1801 agaagaaaat gcaggttgta acgattttgg tgcggaaatc gacggtttac tttcagacga
     1861 acgttgcccc ggttgttgca cagaacgatt tcctgataga gtggctaccg acgaattagg
     1921 aacagagtat tggttcaata actgctgcat tgctggataa ctcccatttt tcgcctgatt
     1981 tttcacttgg cgaatctgtt gtgaaagaga accagcagct gttaattgtg gcacgggtac
     2041 ttttccagaa ggcaacggaa tcgattgacc aacaaaagcg atacctttat ttaacaatgg
     2101 ttttctttga ttattccctt gttgcttact caattgtgcc aatgattgta cctgttgttt
     2161 tggtcttact gaattcatgg acgaatttgg aatcaaacca tttgaaagac cagaattcca
     2221 ctctgttttc gatttggtag aagacatttc aggctctacc ccatgataac gaacatgacg
     2281 accactataa ggcaaggctt gatttgaacg cccacctagt tcaaagggat taaccacttg
     2341 cctccctttt accgattctt gtggtgtacg tttttgttga aacttttgaa atccatgtat
     2401 cgctgcagaa gcgagtggat tcgttgctcg atacacattc cctgctaatc ctgcaccttt
     2461 gattgccaaa ttttgtccag cagttgatag ttgaccaggc agttgtttca tttgagacaa
     2521 agaatgactt gctatgtttt tcgtgttgtt tatagtttga tctgcagctc ctaaccctag
     2581 tttctttaat aaatctttcc gttttacaat gagtaaaatt gataggagtg ctttgacaaa
     2641 catatttcct aaaaaaccaa aaacagtatt cattggaatt aaatcgtcta ctatgttata
     2701 gatcaaaaat gccaccaaaa taatcacact gtaaaaagat ttttgaaaca tcaagcccaa
     2761 aaaggacttt aaggtattca tgagcatata atcaaatact ggaacaaaag aagcgagtaa
     2821 tgaaaaagga atcacaagta ataaacacaa caccaacatt tgaaagataa aacgaatcat
     2881 gccaatcact acaagtggta atccaatact tagattaagc aatggcacca tagaaccaac
     2941 gactgcttta taaacagctt tagttggttg aatatagaca cgataagcct tctttttccc
     3001 tctatcttct cctgcagcag tggtcacttt tgtctcaata tcagataatt cttcacttga
     3061 cgcttccttg gctagaaatt cttgtggttc aatctctgct tgatacaatt cttctaaatt
     3121 tgttgtccca taatttaaca atatatacgg atcaacaaca gccgctttat aataaagttg
     3181 gcgcactttt tgaatggctt ccttttgtgt cgttggcact aaaacaccat tgacatcttt
     3241 tgtcgccaat tcatctagac tatttgtagc agaaaaaata aagccttcta cctccacgga
     3301 caaatcattt aaccctttgg taaaagcttc tcctttacta ggtacgttac taccatagcc
     3361 aaaccaaacc attgaaaaaa tgacaacgaa gagaaatcga ataaacacca tttttgcgta
     3421 atgtttccct ctaaatatac gaagagtaaa tagatataac acatacaaga cgacaatccc
     3481 cgcaccaata ggatcgtata tttttgaata aatagtatcc gaaaaagtga aaaactggtg
     3541 aattaactcg ttcattgcag ctccttcata gagcttctca atgacaaaat caaggccttg
     3601 ccaaatcaat ttacttaaac taaagaacat attcgtcatc atttttagaa aatctgtgcc
     3661 atttgcccca aataacattt tgcctaatga aaattcacta tcagataaat aagaagaaaa
     3721 ggtatcaata tgaaatttcc caatctcata gatcgattga tccacctgct tttctgccgc
     3781 atacaccaat ccaccaacaa aaagcagtcc gaatccaatc gttaaaaaaa acaaaacgca
     3841 ccatttcttt tccttatgct ttatataagc cattgtttca cctcatttct attgcatgat
     3901 taacgtatcc tgttcttcta cttgagagtg acttgttttt tccgttgttt gtaatgcagc
     3961 ggtcatttct tcaaacaaac tgtgtacatt gagttttcct acacgtccat agggatctaa
     4021 catccaacac tgtcctttaa tcatgtggct catttcagct aagttcgctt catttacttc
     4081 caaacgtaaa tgattcaaaa tcttttctcg ctcactgtct tcatcaaaag caaagacgac
     4141 accaaaattc cctttttctt tatctgaacc taagtcacca ataaattgtg tatctaacac
     4201 taattgattc atctgggaac gccccacacg tgccattgct gcaataatac tacgtcctgc
     4261 gttcgaatta ttaaataccc atgcttctgt aaaataaatc gctgttttct tactactatc
     4321 tcgttttcca aacatttcac aaaaacgacc taaactaatc atcaaacaca aagactttcg
     4381 atccgcatct gtatatagtt ctggtcggac attgtcattt ggtaatttca aatcttggat
     4441 ctctaaaatc gttgttttgg cattaaagtc taatcccggg ttttctccat aagaaaatcc
     4501 taaacgtaaa atcgaatctt ctaccgtcaa gcgtaaaaac tcaccatatt ctcgaacctc
     4561 tttttgttcc atttgttcta aatagtcgac cacatgcatc aagccaactt gttgtccggc
     4621 ttctttttga actaaaactt cttggattcc ctttaatacc gcccctttta aaaggtgatt
     4681 ggtacctaac ggacttaact catcaatcat atcctgggct acttgtttcg catctactcg
     4741 ggataaacaa acaattggat ctaatacacc ataattacgg ggatttgtag catctaacgt
     4801 gacataactc attttttgca aatgttctac aaacaaaggg taatgtttct gataatacgg
     4861 gtcttgaatg agtttcgtaa accatttgcg cttttctcct ttgggatcca caaataaact
     4921 ttgtacattc ataaacgaac tatacaaaaa gagcaagcca actaaaaagg tcttcccttt
     4981 accggttttt ccagttactg ctacatgagg cgcatcaaac aacgcacctt tgattccttc
     5041 cgcaatggct agaagattca aaaagacaaa acgatttgac gcataaatta attcttctaa
     5101 tggtgttttt tctcctcgag aaccaccatc ccctaatgta tcttgccgac caatatacca
     5161 gccggattcc atacctaatt ctgatactgc tgaaaacaac cattcgccaa ctgcttctac
     5221 agttgttcgg tgcttccaat gtaactgccg tcccaatggt tgccctaata aacatttata
     5281 gaacaattct cgttgatcat tttttgcacg gtggatatgg ataatcgtat gaagttgttg
     5341 aataaccgtt gcactatttt gacgaacttc ttcaaccgtt tgaccgtata caataaaaca
     5401 tgcgatccaa tcgacgacag gttgcccatt atctactcgt tgctttaaat gttttactaa
     5461 tcctcgaaca gttaataagc gttcactagg ttcttcatca ttttgaacca attccttatg
     5521 ctctgttttg aaccgttccg atagccaatt cagttttaaa ccaatacctc cctgtccttt
     5581 caagcgtgga tagtgaattt tcattcgcaa ttctgctggg aaagaaagat cctgagcaaa
     5641 ttgaaataga tcacaatttc tcatattttc aggtgtttcc ccaataggca acaatgctaa
     5701 ataactactc cctaactcat cttctaattg aagaaggcct ttatgttcct tcggtatacg
     5761 tacatacgtt ccttgattgt ctttcagtac ttcccaatta ttttgttctt ctactttgtg
     5821 agccattcct cgaatatatt gcagacgatt gagatacaac agctcttttt ttactaaggg
     5881 acgaatagaa aagctataaa gtaattgtgt cacttctttt acataaggat cactttcaga
     5941 aaacaaagtt tccaaatcat ctacttctaa tccaaataag cgtgctaaac ttcctgctaa
     6001 atctaaccag attagattta ttacatcatg aaaactctcc accgttgctt tttctttgac
     6061 tttgattcct aaaataaatg aaggacgata gacttgacct tctcgttcca acaatgtcac
     6121 tgtttctttt gcataatacg caccaattgc ttgactatcg ggagaaaatc cctttcctaa
     6181 tgtgtcaaac ttcgtccgta aattcattgt tcgtggatac atcaccaact ccacatcttc
     6241 ataatctgct aaaagcaaca gtaaacgagc catcatttct tttatttcct gtgtcttttt
     6301 tggtgtgttt tttgaaattg ttcgtggaaa aacatgatag taggcccaga tacaatcctc
     6361 tccatttaac aaacaatttt tcttgaatgc ttttactggt tcttttacaa gcaccaatga
     6421 atttcacctc ccaataaaaa aggaacaacc agttgattgt ccctttttct aatcctctaa
     6481 ataaacatac ttattcacaa aaagtaattc ttccaatgca ttgtttgata ccttgtccca
     6541 gttataatac acacaatcac cattcatcat acgaatcata taacaaaacc aatcccataa
     6601 ataaaaatgc actttttttc catcaggttt tagttttgcc agtgctaaca cacataaata
     6661 ggggagtcct atataaaaaa caactcctgc tggtggaaaa aaagaaatga cccaaccaat
     6721 aaatgaacgt aaaaataaat attcaaagat tacgaccaac aaaaaagttg ctgcattgac
     6781 taatgcaact ggaaatggaa gacgccaatt gcttgtaatc tgttgtacgc gataaggttc
     6841 cgcataaaca atcgaataat cataaggttc ccaatccact tcactatcct cctaatccat
     6901 aaataatttt tcaatgattt taccaaacgc atcaaataca cgtgttggat catttaagaa
     6961 gaaataaact gcggcacctg ctgcgataga tattaatgca tttcctactt ttgatttcat
     7021 aaaatttcgc aatcccagat aaacaaccac cattattaaa aaatagccac cttgcgttaa
     7081 tataaaatct acaattccct tcagtgtcat ttccgttctt ctcctttcct atccaaccaa
     7141 ttatgtgtta atttttcaac ataatactga tttgtttttt tcgtaatgac aatcgtcatt
     7201 tgttcttttt gtaaaagtga cgtatcacca tctcgaaaga cgaccgttgc ttttacaata
     7261 aaaggttttt tcgtttcttt ataacgcaat actttcatct ctacttgatc aactttagct
     7321 gtttgattga gtccttcagg ttctttcatc atgtatgaca tatcttttgc attgtttttc
     7381 gcatacttat ctaagaaatc ttccacaaat ccttcaacat ctttcgcttc cgacgcatca
     7441 accgtttcgt ataatgtgtc atcataagta atcattcgtt gtttttctgt attctttggt
     7501 agtgccgtaa aataaggata agcaataata cgacagccct tgtttgaaat agaaaaggga
     7561 agattgagta cttgtgtgat tgtcacactt gtttctttct tactctctgg attcatcgag
     7621 cgaatatcat aagttacttt ataactagca gttggaatgg tttcaatttc ttttaatcca
     7681 ataaacgaag cagcagttaa ttttcgaaca cctgaaacac ttcctggttc ttgaataaag
     7741 ttttgtgcat aaaactcttt ttttaatgtt tcttgacgtt gttgttcttg ttcatgatta
     7801 tcgaatgtat tcatatacaa agcaacaaaa cgattcataa aggattcaat ttcaggtgtg
     7861 tacgttaatt ctgaccgact ttgtacttct ttcttttgta attgttggat ttcatctttc
     7921 aaaaacgaca cttgaacctt cgttttcatg gattgaaaaa atgccaagcc ccctgaaaaa
     7981 caaagaaata aaaccaaaca ccaaatccat ttcactgctt tttttgaatg gattttcttt
     8041 tgcttcggta ctttctctgt ataaactttt tctttccgcc gtagttgtcg ttttttcata
     8101 agctactcct tctagtatgt ttttactagt acgtacgatt gacaacgata agcttgtttt
     8161 tctgaaaaat agtccattca attaccctca ttttctattc cttttctctt tcttttatat
     8221 ccaacaaatc cagcaatact tgtcaacact cctcctagaa cagaaagtgc aacatgtatt
     8281 tcttcaccag tctttggtaa gacttttgtt tttccatctg ctgttttgat ttcaatattg
     8341 ccacttggaa gtttctttac aggaatcgac aaatccttta ccaaattccc ttcactatct
     8401 tggattaatg gaataccatc ttttacacca acaattgttt gatcacctgc tacttttaat
     8461 ggggtggttg gtgttggaat atgggtcaat tcctctacat tactagtgac aatcggtaca
     8521 ctcattcctt gagaagtatt cgtcgtaatt tcaccgcttg gggttaccga tactgtgact
     8581 tcttttgctg aaacaatagg tttattttct tttggaattg cttctgattt ttcaacaggt
     8641 tgtttcggtt cttctttttg ttcttcactg gtagacgaca tcgttggctg ttccgtactt
     8701 gacgaatcag tattcttttc agaagaactg gttgttcctg attgttccgg agttgtgact
     8761 atacttgaat ccgttgttgt tgaactatgc acaccttctt cttcgatcga atcagtcggt
     8821 aaagttgatt caggaaactc tggttctggt gtcactggct ctttttcagg tttctctgac
     8881 tgcaattgtt caggttctgc tggtttttcc gttgttcctc catttagctc tgtctcaaca
     8941 tctggtaatc cttcccctgg caatgtttct acaatttctg acttaggtgt ttctacagtt
     9001 tcatctgcat aaaccaatga actccctaat tgaaacgaaa ataatgaaat tcctagtgtt
     9061 gttacgactg ctaatgttgt ttttttcatg attattcctc caatttttta tgcaattcgt
     9121 gttttgttca tagacgccgc actaatatta tctgaacgat aaaagaactc ccgtccgttt
     9181 tcttctaacc caacatatgg attatttttg aataattgaa tcaatagttc actggtgttg
     9241 tattttggat agtttgcaaa aaaataatac tgtgcttgtc gcccatccga aaattcaaca
     9301 agtaattcat aacgataccg cttcaatcct tttcgttgtt ttaaaggatc tctataccaa
     9361 cttgtccatc gttccattcg tggatagaga tagacacgat acacatagaa caatgccata
     9421 ccacatacca ccaaaaataa taatcgccac ataaaaattc ccccttttct tctacctttc
     9481 ttgaatcatc gaacattcat accctctata gagatttgaa aaatccatca tcaattgttc
     9541 atataaacgt tgttgcaaat agcgattgtt tcgaatacat aattcttcat agccatcaat
     9601 cacttgatct tctttttcat agcatcttgt agctctatga cgctcagctt gcaagacata
     9661 accaaactct gcaccatatc gttctactgt taataactgt tgactacgca ataatgcaat
     9721 ataacaatca caaaaatgtt caaccgacat ttgatttttc tcacgtttgt gaaataggta
     9781 ttctttagaa agcacattat tcgttaaaca atctgataaa tagcgataat ctggcggtcg
     9841 gttattttca tctgtgaatt tttcccataa taaactgatt tttattcgtt cagcggctag
     9901 atagtcttgt tcgcgtggat aaaattcatc aatcgcacaa agttttttat cacctgaata
     9961 aacaaatcca tataatgcga aacgaactct tccttttcaa ttggctctcc taaaaattcc
    10021 agtgacatac catcttctcc ttcctaaaaa aaataaaaaa gcagaaaccg atttggcttc
    10081 tacttttctt tccttttata gctctttttt acggaagaat ccgcaaatga cattttttca
    10141 aaaaatcaat cggcacaagt tcacgaaaac ttggagcaat tttggataga atcacttccc
    10201 catcttgtaa aatacaatca taaaaatcat cagttacttg atacttacgc ttcttacaat
    10261 aattgataaa atagtagcga tcattttcta tttccattcg ttgttctact aagcgcatac
    10321 gaacatcatc atagaaataa gccacaaatc gcccttcaag ttcatgtttt tcaatgggat
    10381 actcttctgg taacaatcta ttttttcgtc gtttatacat tgctggatac cttagaagaa
    10441 gttcatctaa acgtctgtca gtatatggta actgacgaat caacgattct aatcgttctt
    10501 tatacagata gaataattgg cgcaagcaat agtgtaccgc ctctggattt tctaatactg
    10561 ttggatcacg tgaccaatac gcaccaacat aaatcataat ttttttcatt tagacaatca
    10621 caccagcaat actaatgacc gatccacaat aggtagaaca agtggcatag cgaccaattt
    10681 cctctaattc tatctcaatc tcttctaaag aaagatatgt ctgttcttct gttggctggc
    10741 gactcatttc ccaagtgaca gtcaccaatg aaatttcttc cataagaggt ttttcgctgc
    10801 taagtgctgc cacacagttt tcatatatct tcattttatc gcagcctttt ttaaccgcta
    10861 aaatcgcagg tattggcaat gaaaatcgat aaaagcgaat gaattctagc cttcctttcc
    10921 ttcggctaaa ttccaactct tcgagttctt gttcgtatcc cttacgaact tctaataagc
    10981 gtgcaatttc ttgccagttc tctttttcct gataacgtcc agcttttacc gcttgttcaa
    11041 tataaggaac tcgaaaacga aaataagaca aatcaaccat cttttcattt aatgcttcga
    11101 ttgcagcctt taaatccaac aggcgcagcc attctgttgt cgtatatgca ttcatgcaat
    11161 tttccctcct gacgttccca tccattctag atagttttgt tgctcgtcat ttagttcggc
    11221 attatcaatc ccttcttgta caaagtctct tcctaaaata cgatctgctt cttgtaacaa
    11281 ctttaatgtc ggagctactt gtttcaaaaa ccagcgttct gaacgatcta atgagaattt
    11341 ttctggcttt actgtaaaat ccaattcttc catcgaacga aataattttg cccatcgttt
    11401 atcaacaatc cgtgcatcac tttccagtct atcccaaaca ataagttttg cattaattaa
    11461 accaacgcca atctctccta aacgtttcgg tgaatcacct aaaataaatg cttctactgc
    11521 ttgtgttgct ttctcattgg ctaaccgtaa ctcatatcga ttataaatac catatttcgt
    11581 taatgcttct aattctgaaa ttttttctcg gttcgcaatt tcaaaacgtt tctcataaaa
    11641 attaaaaaag agaggactct ttcgcgatcc aaaatacaaa gataatcctt cacttcgaaa
    11701 atagccatca tctaaaaaac aaccgccaat attctggaat ctcttaaagt ccatttctaa
    11761 taaacctgct ttttgttttt ctagtaattc aaagagatcg aaatgttctt ccgtttccaa
    11821 tccccaaagt tcatctaacg caatatcgaa tcggctacat tgcacacgtg tataaccagc
    11881 ttccaacttt ccttgatttt tccaaatgga atatagccat gttcgccaat ctgtttgttg
    11941 ttcacgcatg acttcctcat attcccggca accttgtcct gaaaattgca caagtacatt
    12001 ttctgttaca gttcgttttg aatgccagaa cacccagata ttcccacgaa cagaaagaca
    12061 gtcaaaaaga tacaagcccg ttggacgtgt ttcaaattct gtcagatcca ttcgtaatac
    12121 acgttgaatc actgttttgt agtctgtccc aaaaaaggaa agacgcaaat aatcaatcat
    12181 tgctcgaaga tctattccgc ttttcatata caatcgaaag cgttccttga ttcgttgttc
    12241 aaaatcctct gggatttttc ttttcccttg ttccatcttt ctaatgtaat cttttgagca
    12301 gcccatcaat tgcgcaaatc ttgcttgtga tagcggcttt ttatctccac ttatattatg
    12361 aattgcactt aacttctttc tgtagctttt tagctcaatc ggtgtcattt tctcgctcct
    12421 tttataaaat aacaaaatcg atttttttaa ccctttagaa acaccgatat atcaactagt
    12481 ccccctcgtt ttttgggaaa agtagtacaa ctgctgacct tagataatca cttgatacaa
    12541 aagggtttat aaaacttttt tctatgtgtt atgttatttc tgccccccat attatatatc
    12601 ggggggcttt acacaaagag gccgtgcgca agcgcacgac ctctttgtga gctgcacacg
    12661 ctgctagcga gtgcgcatta taggcccatg cttcgcagcc ttaatgcgca ccgccaggca
    12721 gagtgcggct accagactac gtctagagcc ctcccgatag ataataacta caaaattaaa
    12781 tgatctaatt ttgttatttc actatatttt tcaggagaaa aaacgcgata aattgacaaa
    12841 gttaaatcct cttctaaatc tactcgataa tgatacaatc gagtcaatcg ttctatatct
    12901 tttttcgtta gcaatacttg attaataact tttcctgtaa aaacacgttc tttgcgcaaa
    12961 aagcggtaat cagttacttg ttcaatacga ttcaaccaat cataaaacgt tctggaagaa
    13021 agagaaggat taatcattcg tattgctgta gctactctat aataacaact ctctgccatt
    13081 taacttcacc tccttagttg attacaaaat atgcgcatca gaaaaacttg ttccttttgg
    13141 atcttttgct aaatacattt ggcgctctag ttgttcttgt aacaactgat tatcatctcc
    13201 catcgtttga tcttgactat cctcttttac tgtaaaatca tagacaatca tttcagtaaa
    13261 tttctcaaat tctttgataa agtgataatc tttgtaaaca aatggcgcaa aaaactctac
    13321 aggtacatca aatcctggaa aagcatatcc acgaccaggt tgatctttgt tgtaaaatgg
    13381 cttattttta aaatcttctc caaaagtcat taaataacca atgggagaca atcgaccaac
    13441 agaaatacgg aatgttaaat tatcccgaac accacttgga aaatattctg catctggtcg
    13501 ttgcattgct aaaataatgt acatccctgc ttgacgaccc tttaatacta atggctgaat
    13561 caatttatcg actctatttc tcgcttccat atccaacgaa ccataaaaag ctgcccattc
    13621 atcaaaaaag ataaaatgcg gtggtaaccc ataataagca taatttttcc ctggttcata
    13681 tttaggatct ttcatggttt tcatataggc ataccgttga ttcatttctt ttaatgcatt
    13741 tcgtaaacaa ataatcattt cttcatttat tttgtaaaac acatgtcctt tgaatacatc
    13801 gacgtcacct aatgctgcta aatctgccgc ttttccatcg caaatatcta ctgtcccaac
    13861 acttaaacaa gcttgaatta aagaatacaa taaataggtt ttcccaccac cgattgcacc
    13921 agcaatcaac atatttggtg attgatcata tacccaatcc aaagttttca ttaaatgaat
    13981 acgtccattt tctgctatac attgatcaat aggaatccga ccagaatctg aaaggaatac
    14041 ataattgatt cgggcttttt ctcgttcttc acgaattaaa tcacgcaaaa atacttcaga
    14101 taacaaatga cccattttta aaaatcgttc gtgaaaacgc ccaccatcta aaggaaaaga
    14161 aaccactaat tcttgtttct tttgataaaa ataaactttt ggtaatctca ttttttccct
    14221 tgtttgtttc tcactaactt gtttctttgt ataaacaaac cgattgtcaa ttaacattcg
    14281 aacaagtaac tgacgccaat aaacagaggc aaaatatcct tcttttagtc gcgaccaatg
    14341 catcaaccct aaaattaacc cactaaccag caacaaatac aacacaatac ttattccagg
    14401 aataagatac atttgccaat tcgaaaacgt acttaaacgt ggataaaata ctttccaact
    14461 aaaccaacct aacagaatca aaaatggaaa caaaaagaaa ccataatagc aaaaccataa
    14521 ataacgattg aaatagcgaa ttcgccgacc acgatacttg aaaaaatgag ctttcaattg
    14581 tcacattcct cctttctgtt tttacttttc ctgtgctaaa aacttttgac aaaaagcaac
    14641 taatacagga atatcacatt tagtaaaaaa acataatcga aaaagattta atcgattttt
    14701 ataagggtgt gcacacatat tacatattcg tactatacga atccaaatca cttcctgatg
    14761 atcttgtttc cactggtacg gtaatgaaaa acgaacaaga tcacgtaaaa acatacactg
    14821 cccaataacc gcacaaatat atgcatttgt ttgtacaaaa atcgttggat tgtctccaaa
    14881 atactcaatc aattgttgga tattttgata acaagcacaa tctctcaaag tatttgattg
    14941 gttttatctg aattcaaaat ctccccttcc ttctatctta gataaaaaga ataggaagac
    15001 tccttcctat tctttcaaaa aaaccttatt ttttctcttt ttccttagaa tcaacacttg
    15061 gttgtttttg atctaaacct ttttcttgtg attgtttcat attaggatta tttgttggag
    15121 cttctacttt ctctaatcct ttcgcataaa tcactaaatc tgaatattca cgattgccat
    15181 cttgtgctgg aatatatacc acatgatatt ctggatcaat taatttcaca ggatcacgtc
    15241 gtttaaaacc agtcaaatct acatctgctg gaaatttaac tcgtcgtgca atctttggtt
    15301 catctggatt tccaaaaact aattgtgctc gctgtgatcg aatttctccc gtataatcgc
    15361 ctgtttcatt atcaaataca cgatcagact cttctaatgc gccaaagcgt acggctccta
    15421 aacaagtttc atctaatgct tctgtaatgt cataattatt tggaaatttt aatcccatct
    15481 tacaaaacct ccccaatttt tacaccagtt aaaaagacac caacatcttg atagccagtt
    15541 gttcgacgct ctgtttgtcc catatcattt gtttcacgac tattcactcg ttctttacga
    15601 aaaacaaaat aagcttcttc aaatgaaata cgcacctctt tttctggtaa tcgtaatctt
    15661 ttatcctctt cagctgtgag ataaacaaaa aaagcaggct gttcttctcg cgaacactct
    15721 gcttgtaatt gaacatatcg cccagacaca ccattaattt cctttggtgg attaggttcc
    15781 cgtgaaccca ataaaaatac ttgacctaaa aactctttct tcatacgcaa tgctttttct
    15841 gccataaagt ttttcctcct cttaaaaacc aaaataaatt ctaaatattt ctacattaca
    15901 atcaaacaag cggttaaaaa tcctaaccgc ttgttcctca tgactaatct ttggctttac
    15961 gtactttata aagtgttata cctaaagcaa tgactaatac tactaatcca agaaccataa
    16021 gtatactact tgtttgttca cctgtttttg gcaacattgg tacaacagta gctggtttaa
    16081 cccgttgttc tggtttcact ggttgcggtg gttttggcgc ttccacaatt tgaaccgttt
    16141 gatctttgtt tgttagatct acattgtgtt tcgccacttc ttcaaacgtt tctttctcat
    16201 cttccgtttt cgcttggagc cattccgcaa aactcaagcc ttttcctgca agattgcgac
    16261 cgtccactaa gaattcaaat aagaattcca tcatttcttt atcggccgca aagacttttt
    16321 ccgcatgagt gccttcaaat ggtgtgccgt ctggtgttaa gtagccttga acaattaagc
    16381 gatagatttg accaatcgca agaccgtcta ctttgaccca ttcaagcaca gtttgatttg
    16441 gcaaggccaa gatctctttt ccgccatttt tattcgtcgc tttcgtcgta atggttggat
    16501 tactgatttt gatggtttgg tcattgtcgg taatatctgc gtgagaagcc aataatacgc
    16561 cttctctaaa cagatcttca aagaccacga cttctttgcc tcgcaacgca cgtgcgtcta
    16621 gaataaagtc tagattgact gtaccatccg ttgtttctgc cacaaacgtt aaggtacttg
    16681 tgacttcttt tccatctact agcactggtt tttccgttac tttgtccatt agaacaccgt
    16741 tgactgtgta tgtttttcca acgaccaaat cgtggtattt cacagcgtct gtaatggtga
    16801 ctttcgttaa tggattaacc tctttcaagc cagtgggttt atgtgtagcc gttgtacgta
    16861 cacttgggtt agttactcgt accgtttgcc ctttgtcgtt gatgtcttta tggtttgcta
    16921 gttcttcatc atttctaaaa agactttcaa acacgacgat ttcacgacca tttatacctg
    16981 tcaaatcaaa ggtaaatggt acgatcaccg tttcattttc tgctttggca ataaagccaa
    17041 cactcgtttc taccgtattt ccatgcacaa gcaagggttc accggttgct ttatccatta
    17101 agatcccttt gaccaagtat gcttttcctg gaagaatctc ggtaatttca acggtatcgt
    17161 ttaaggtaaa caatcctgtt gggtctgcga ctgtcaatcc gtcttcaaag acagccgtgg
    17221 tatgaacggt tggattattc actttcacac tttgggcttc atcgttgata tccgcatgtg
    17281 ctgcaacttc aatgccatca cggaaaagtt tttcaaaaac aacgatcgtt tgacctttca
    17341 gaacactagc attaaccaca aattcaaggt cttttgtgcc attgactttg tctgcaataa
    17401 aggccaaggt acttgtgatt tctttgccat cttgtaaaat tggcgcattc gttgccttgt
    17461 tcatcaatac tcctgaaatg gtgtattctt ttccgacaaa caagttggta taagaaacac
    17521 tgtctttaat gacgatcgtt tctaatggat caaccacttt ttcaccatcg gctttatttg
    17581 acgcttgtgt ttctagtttt ggtggaatca agtaattacg tacagaaacc actaacgttt
    17641 ccccatcatc tggcgcattt tgtttgaatg caaccgtgaa tgcttgttta tctgtatttg
    17701 gttggaaact ttctaatggg gcattttcca agaagtaata gtcatcggca actagacctt
    17761 tcactaaaat ttccccgttt tgatcagtgg tatattcacc aattgcttga tttttaccgt
    17821 tatacaaggt aaagttcaca cctgcgagcg gcgttttctt cttgttccat tcttcatact
    17881 tgaataattt tacacttttc cgtgcttgac ggttcgcaaa atggatcact ttgatcggtt
    17941 ctgtatcatt ggtaatcgag aacactaatg ggttttgatt caaaacgtaa tttacggggg
    18001 ctttgatttc ttccaaacga tagcgatctt ttccataagt aagtgtaccg ttagttagga
    18061 aaaagccttt ttcattggtt ttaaagatat ccgttgtttc tgtatcattt ggaatctcca
    18121 ttgtgacata acgattggct aaagtatcaa aaatcttgaa ttctgtatta gcaagtggaa
    18181 tcacttttcc tgtttcttga tccactttca ccactttaag acgttgttca attactttat
    18241 cttctaaaac ataatggaaa gtttgcccat tttcagaaat tgtgacttca agtggttcaa
    18301 atagtttata cccttctttt cctttggttt cagaaagaat gtacgtatca aagacaacgg
    18361 tatttttacc aaaagtagca taaccatcat tgtctgtcac aacggttttc accactttgc
    18421 cagttgtttt tgacgtcaaa gtgatttcaa tgccagctaa ttttggtttg ttatctggat
    18481 tcgttgaatc gactaaatct ttattagcaa ctttcactaa atcaatggtt cctttgatca
    18541 cttgatcttt tgcggtcgca ttgacagttt ttccaggttc aacaacaatt tcaattgctt
    18601 catcagaaag cacataacct tttggcgctt tgatttcttt taaaacatat ttgccaggat
    18661 caagatcttt aatttctgcc tgtactttgc cgttaatttt tttcaaggta atttctttaa
    18721 tctttttccc atctgcttga tacaaaccat agaccgcatt ttctaaagaa gctgcacctt
    18781 gtgctttcac accagtttcc gcatcttctt taaacacacg aatgtttcct tgttttagaa
    18841 tttgtaaatt aataacagac gcattaggtt ctgttggatg taacacacca attgtttgag
    18901 ctcctggacg acgataggct aatggcgcac cttgaacttt aattttattc aattgaattt
    18961 ttgctttatc cggtgcctct tttgtgactg taatccatac ttcattgccg tgaatagata
    19021 cattgacacc ttttggtttt tgaatgtctt tcacatattt caaaaaatca tttttattct
    19081 ttactttcaa cgtttcgcca atttttaccg tatgtgtttg attattaaaa gttgcttttt
    19141 caccaaagtt attaatcaaa cgttgaattt ctgcttttcg acgatcataa tcaacacgga
    19201 tatttttgat tgggattttt gtgcccaaag cttcccaaat cataaattgt gccgcaatgt
    19261 attgttcatc ggatttgtct ttatttgtga catatcctaa ataagagatc aagcttgttt
    19321 ttttccgtac atttgaattt ttcaaatagt ttttcaaagc agtagctgta taaacttgac
    19381 cttctgcggc tgagacacca ggctcaatac aaaacgccac ttgtccatta atctcaaaac
    19441 gaaccatata atttaaagtg aagtttagat tgttcggact agaagtaaca ccaatatttt
    19501 cgccccaatc aattttccga ccacctaaaa cagctgcatt ggttgcacgg cgaacacgaa
    19561 ccgaacgatt ggttggtact gttactaaca gtgtaggtgt acctgttttt ggaatgttct
    19621 gctctttttt cttttgattt tcaatactag ccgtagattc tttgtttggt ttcgttggtt
    19681 gattttgact agctgcggtt gaagagattg ttttttcaac gttttcctca ttttttcctt
    19741 ctttgtcagc agtagccgct ggttgttcca ctgtttcttc tattcgtttc ttttcttccg
    19801 cttgtttctc agaagactct ggctgcacag aaggcgttgc ttcggcagct ttttctcctt
    19861 ctagagtgga cgtttctgaa gcaggggttg taattgaggc agtttcttca actggttgtg
    19921 aagtaccagg actattccct gggtgttgtg tttccccaat cgtctgatcg agagtcgttt
    19981 tggaattgac cgttggcgct tctgtcacag gggcctgttc aacagtggct tcaccagctg
    20041 ggaccccggc ttctgctggt tggacttctg tcacttcaga agcgacttgg gcaatggctt
    20101 ccatcgtagg ttgtatagcc gtcgtttctt ctgcatgtgc ttgagcagtt cccaccaaga
    20161 aacagaattg agaaaaagcc accaatgcga gtgctttcaa ccaattcatt ttcgtttttg
    20221 catacacaag aattcctcct tttcctgaga attttatata ataaaacaaa acgccgttac
    20281 caaaagtgat aacgacgtga actgtctctt ctatttttat ctggcgctct tatctgaaca
    20341 catttcatca atgattttac cctcccacta taattacaga acaaaaaaag aacctgcttc
    20401 gtggaaatca actgtccatt tcccaattgg aataccagct tttttggcaa cttcaccatc
    20461 accaaatctc atgccatacc aattactacc ttcatcctta atttctttcc aaccatatga
    20521 catagcttca tctaatgaat caaaaatcat gacactattg ccaattgtta caggatctgg
    20581 cttaagatct tctggaagag aaatatcaat cataccataa tgatccgatt cctcagtatt
    20641 attgtcatta ctagctactg gtttggttgt tctactggct tattttgata caacaagtac
    20701 tcttattctt cttttacttc tccgttttca atttcccatt caaaccactc cgcaaagtct
    20761 tgcttaaaac catctactcg atacctattt tgtaaatagg catcttctga cgtcgtagga
    20821 tctaaatagg taggtttatt ctctggacga gcatagacac ctacccatgt ttcgtaatct
    20881 ccttcaaaat aactatctac caaatagcct ttttttctaa gaatctcccg aacttgattt
    20941 ttaattattg atacttgttc aaaaccatca ctcattctat cacctcaaat aactctagat
    21001 tctcataaat attcccgaca atttcaatcc tatcaatgat ctctaacaaa tcgctactta
    21061 tgcttcccca ttcgtataag aatttccctt ccgcaaaata aattttccat aaacttcttc
    21121 atgatcgtcc caaccaatat cactctcgaa aaattccacg ttgttcttat cttttagctc
    21181 agtaaatgcc ataagttcaa catgttttcc aacctcgaaa caatcacacc acccatcaac
    21241 cgttacacgt gtgccttcaa tacctaagaa actttgtcct cgtgtattaa acactaaaac
    21301 tttccgcatt ttttgctcat taattaatca cgcgcgaaat ctaggaatca caacttattc
    21361 gcttcttctt tataaataaa aaaactccaa taatcggaaa ttactatctt ttcctcattc
    21421 cttttttaaa tcttcatact tcttcttatc tttgtcctaa cctaataaca caaattttga
    21481 agaaaaactc agtaaaacct gattcttttt ttatcttcac aacaaccaac tttttcttca
    21541 gatcctttta ctagaacttg tatacaaaaa ttgttgaatg ctgatataat ttgcggctac
    21601 ttgaaaaaac gatagatgta aaaagataaa tagaatcatt ttttattcta tatcagtcta
    21661 tgattcattg cttatcaatc acccaaccca ttcgatgccg ttttttttag agaaatttaa
    21721 aaaagaccgt acgaatctcg cattatccga ttacttttcc agaaaagatc cccaattctg
    21781 tataaataac cctatcttct tgttggtttc attttctcat ataagaattc agtcaatcta
    21841 cccactatga ccattactac gtccaacaca actgacatat taaccacgta agtattccct
    21901 cacagatctc cgaatctcca tggtatggat tcccatcctg acgctgcaga ggacaacata
    21961 tttcatgtgg ctctgtaccc ttttgatttg gaaacactac aaatcaatac tcatctataa
    22021 cttatcaagc gaactttcgc taggcttcta gtgattacta aattgttgga taacatacat
    22081 agcccatttt atctagctgt tattcataac agttagttct tgtattactt cttccgcttg
    22141 tgttgccatt gagcttgtca aaaagttact tagaaaaaag ctgccagtaa agcaactttc
    22201 aacaaaccct ttttgtttct tatcatatca agaaacctcc ttttataaat tttttctata
    22261 ataaaaaac
//
LOCUS       DAYYAJ010000038        22194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000038 DAYYAJ010000000
VERSION     DAYYAJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.038"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..2094
                     /gene="tdc"
                     /locus_tag="ACHATZ_001234"
     CDS             259..2094
                     /gene="tdc"
                     /locus_tag="ACHATZ_001234"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8403777.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2399..3877
                     /locus_tag="ACHATZ_001235"
     CDS             2399..3877
                     /locus_tag="ACHATZ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8403778.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3843..5174
                     /locus_tag="ACHATZ_001236"
     CDS             3843..5174
                     /locus_tag="ACHATZ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8403779.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5288..7840
                     /locus_tag="ACHATZ_001237"
     CDS             5288..7840
                     /locus_tag="ACHATZ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8403780.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7847..9286)
                     /locus_tag="ACHATZ_001238"
     CDS             complement(7847..9286)
                     /locus_tag="ACHATZ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403781.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9261..9443
                     /locus_tag="ACHATZ_001239"
     CDS             9261..9443
                     /locus_tag="ACHATZ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403782.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9632..9994
                     /locus_tag="ACHATZ_001240"
     CDS             9632..9994
                     /locus_tag="ACHATZ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8403783.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10181..11038)
                     /locus_tag="ACHATZ_001241"
     CDS             complement(10181..11038)
                     /locus_tag="ACHATZ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8403784.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11158..12069
                     /locus_tag="ACHATZ_001242"
     CDS             11158..12069
                     /locus_tag="ACHATZ_001242"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8403785.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12076..13727
                     /gene="mdcA"
                     /locus_tag="ACHATZ_001243"
                     /pseudo
     CDS             12076..13727
                     /gene="mdcA"
                     /locus_tag="ACHATZ_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13829..14128
                     /locus_tag="ACHATZ_001244"
     CDS             13829..14128
                     /locus_tag="ACHATZ_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8403786.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14118..15794
                     /gene="mdcD"
                     /locus_tag="ACHATZ_001245"
     CDS             14118..15794
                     /gene="mdcD"
                     /locus_tag="ACHATZ_001245"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8403787.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15798..16391
                     /locus_tag="ACHATZ_001246"
     CDS             15798..16391
                     /locus_tag="ACHATZ_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8403788.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16415..17491
                     /locus_tag="ACHATZ_001247"
     CDS             16415..17491
                     /locus_tag="ACHATZ_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8403789.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17516..18385
                     /gene="mdcB"
                     /locus_tag="ACHATZ_001248"
     CDS             17516..18385
                     /gene="mdcB"
                     /locus_tag="ACHATZ_001248"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8403790.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18496..19260
                     /locus_tag="ACHATZ_001249"
     CDS             18496..19260
                     /locus_tag="ACHATZ_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8403791.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19749..21842
                     /locus_tag="ACHATZ_001250"
     CDS             19749..21842
                     /locus_tag="ACHATZ_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8403792.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21867..22169
                     /locus_tag="ACHATZ_001251"
     CDS             21867..22169
                     /locus_tag="ACHATZ_001251"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8403793.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7185 a         3790 c         4729 g         6490 t
ORIGIN      
        1 gaaggacagt cattttattt gtttgttcgc tttttttagg ttatctattt tagaagactg
       61 atttattgaa aaattgttcg cttttttgcg ttgaattatc agaaaaatcc gactttattt
      121 atttttgttt gtaaaaatct acgtggattt gatgttataa aaaaaaaatt ctcatttttg
      181 gcgctatagt gaagaggtgt tacagataaa cacatgtatc tgcaagacat tgaccaacaa
      241 aatatggagg gaagaaaaat gaaagatatg gatatcaagg ccgtctttat aggcgacaaa
      301 gctgaaaatg gaccagttta taaaatgttg ttgaacaaga tggtagatga acatcttgga
      361 tggagagaaa attatattcc ttcagatatg cctgcaatta gcgaaggaga taagctcact
      421 ccagattatc tagccacccg cgaccatatg atagaggtct tggatgaagt cagtcagcga
      481 ttacgcgcag gatcgatccc atggcattca gcaggccgtt actggggaca aatgaatgct
      541 gaaacattga tgcctgcttt attagcttac aactatgcga tgctgtggaa cccaaataat
      601 gttgcattgg aatcttctat ggcaacttcg caaatggaag cagaagtagg acaggatttt
      661 gcttcattat tcaatatgac agacggatgg ggacacattg cagcagatgg ttctattgct
      721 aatcttgaag gattatggta cgcccgttgc atcaaatcga ttcctttagc tgtcaaggaa
      781 gtgctgcctg aaaaagtgaa aaaaatgtct gagtgggaac ttttgaattt atctgttgaa
      841 gaaattttgg aaatgaccga aagctttacg gacgaagaaa tggatgaggt caaagcagct
      901 tcttcccgta gcggaaaaaa tatccaaaga ttagggaaat ggctggtccc tcaaacaaaa
      961 cactattctt ggatgaaggc gttagatatt tgcggagtcg gcttagatca gatggttgct
     1021 atcccagttc aagaggatta tcgaatggat attaatgctt tggaaaaaac aattcgtgaa
     1081 ttagctggac aaaaaatccc aattctaggt gtggttgctg tcgtaggaac gacagaagaa
     1141 ggtcaagtag acagtgtaga taaaatcgtc caattaagag aaaggttaaa agatgaaggg
     1201 atctatttct atttacatgt tgatgccgca tatggcggct atgcgcgttc actgtttcta
     1261 aacgaagcag gagaatttgt gccgtatgct tccttagctg aattctttga agaacatcat
     1321 gttttccacc actgcgtaac gattgacaaa gaggtatatg aagggttcag agctatttca
     1381 gaagcggatt ctgtcacgat agatcctcat aagatgggct atgtccctta tgctgctgga
     1441 ggaatcgtaa tcaaacataa aaatatgaga aatatcattt cttatttcgc gccatatgtg
     1501 tttgaaaaat cagtaaaggc tccagatatg ttaggagctt atattttgga aggatcaaaa
     1561 gctggagcaa ccgccgccgc agtctggaca gcacatcgtg tattgccatt gaatgtgacc
     1621 ggatatggtc aattgattgg ggcttcaatc gaggcagctc aaagattcag agaatttctc
     1681 gatcatctga cttttactgt taaaggaaaa acaatcgaag tctatccatt gaatcatcct
     1741 gattttaata tggtcaattg ggtattcaaa gaacaaggct gtacagattt gaacgctatc
     1801 aatgaattaa atgaaaaaat gttcgatcgg tcttcttata tggatggcga tgtttatggt
     1861 gaacggttta ttacttctca tactacattt acacaagaag attacggtga ttctccgatt
     1921 cgttttgttg aaagaatggg tctaacgaaa gaagaatgga aaaaagaaca gaaaatcact
     1981 ctgctacgtg cggctatcat gactccttat ttgaatgatg atcgaatttt caatttttat
     2041 acaaaaaaaa tcgccaaagc aatggaaaag aaactaaacg aaatcatcca atagaactga
     2101 tttttgtgtg aaagaataac aagagacata tggagaacag acaattattt gcttcatcaa
     2161 aacaattgta tttccccctg tttttcatat attcctcgcc aagatattct tttttataaa
     2221 acgttctata agttggatcg ttcgtttcat gtatcaaacc cagttcacta agatcgttca
     2281 atggatgttt cggtgatact gggttttgta ctgaatcgat gaaaccttgt catttctgtt
     2341 cttgaacaaa aataaaagaa aacaaaaaca tatgtcgcta ttgaaagaag gaataaagat
     2401 gaaagatact ggttcatcaa agcaaatcac gaccatgacc tttatcggaa tgacctgcgc
     2461 ccttgtagca agtgtacgaa atattccaga tgtagcagca acgagctgga cgatgatttt
     2521 ttacatgctg gttgcagtat tattgtacgc ttttccaatc tcattgatct ctggtgaatt
     2581 tgctggaatg tttccccaaa aaggtggtcc agaactttgg gtatcgaatg ctctaggaaa
     2641 aaaatggggg ttcgttgtct catggctgtt atgggtccaa atgtttcctg ggatggtgat
     2701 ggtcgcttca gcactcgctc cgttgtttgg taatattatc gataatgtgc cgcttggttt
     2761 gaacagcaaa tttacattag ttgtcatttt agtcgtttat tggatcatca cttttttgaa
     2821 tttgaaattt gatatggcaa aaatcggcgg aaaagtcggt gtttggttag gattgtacgt
     2881 gcctttgacg atgatgctct tattagggtt tgctgcgtgg atcaaagttg ggatcgtacc
     2941 aaccggaaca ttaggcagtt tttcatggga caaattaata ccagatacta cgactgcatc
     3001 ctcatttgtt tattttgcgc cgatcatgtt tatttttact ggtatcgaaa tgtcctctgt
     3061 gtatatcact cgattggaaa atccagttaa aacgtatatt cgcggagtat ttgccgcgtt
     3121 aatttttata tttttcgtca atattttgaa tgctttagtt gtagcaaatg tcgtaccaaa
     3181 aggacaaatg gaattgaata atatcgcgca atctatttcg atttattgcc aaattcttgg
     3241 tttgccacat cttattgtga atctgtttag tttacttgtc ttcattggtg tagcagtcca
     3301 attatctgct tgggcctcag gaccagcaaa gacagtgaca gaaagtgcta gaaaaggtgc
     3361 gtatccacca aaattcaatt tttggaagac gaatcaattt gacgtatcca aatccgttat
     3421 tttgactcag tctgtcatta tctcaatctt tgcacttttt tatttgctga ttcctggcgt
     3481 caatcaagca tttttgatgc tcgtaaattc aactactgtt atctactgta tcgtctatgt
     3541 catcatgggg atcgcagttt tgagattgcg ttatacacat gcaaagttga atcgaccatt
     3601 tagaattgga aaaaaagaga aaaagagcaa tcttggagtt tggactgtcg ttattgtttt
     3661 atttgcggca atcggatttt ctgtcggact gacgatgaaa gctggaacat ggatcaatct
     3721 aattgcagtc acagccatca gtgtcatttt gtttgtagta ccactctgta ttgaaaaaat
     3781 aaagaaacct tcttgggaac aggaagtcgc catgctgctt gaaaaagaaa aaggtgaact
     3841 tgatggaaaa accggcaatg aaacggactc taactaatcg aaatatccag ttaatcgcat
     3901 taggtggcgc aattggaaca ggattatttt taggttcagc aaaggcaatt gcatcagcag
     3961 ggccagctgt tattctcgtt tatttgatat gcggtttggc tatttatggt ttgatgcggg
     4021 ccatgggtga tttattttta gcaaatagta catttaactc tatcagtgat ttcgttgact
     4081 attatttagg caaaaagtgg gcattttttg tcaattggac ttattggctt tgctggcttg
     4141 gagctggact tgcggaatta acagcaatag gattgtatat ccatttctgg tttgtacatg
     4201 taccagcttt gataagcgga ggtagcgcac taatgatctt aatggcagtc aaccttgttg
     4261 cagtcaagtg gtttggtgaa ttggaatctt ttttctcaat catcaagatc ttggggattt
     4321 tcctttttat cttattgggt ataggattga gtgtctcaga ttttcatacc cagtctatcg
     4381 gaatcaaaga aaattttgga cagatcgatg aattactacc gtttggcttt gctggaatgg
     4441 tagtctcatt tcctatggtt ttattcagtt ttgctggaat tgaaatgatc ggcttaaccg
     4501 ttggcgagac aagtgatccg caaaagaatt tgaaaaaagc aatcaactgt ctgccttggc
     4561 gtattctctt tttctatata ggaacgattt tgtcgatttt atttgttgtt ccatggcatt
     4621 gtttagattt aaaagaaagt cctttagtga ctatgctgca agtgatccat tgccaaggcg
     4681 gagcagcaat tgtcaatcta atgattttgg ttgcttcttt gtcctcagca aatagtgcag
     4741 tctacagtac tagcaggatt ttatatcaaa cagcaaaaga aaaaagtttg ccccattggt
     4801 tttgtgcgct ttctaagaaa ggcgtacctt tacacggtat tttaattaca ggaatgttat
     4861 tttttgccgg tctttgtcta catttttttg tagctgatag tcgattgttt tttcagcttt
     4921 tggcaggcat gacaacgagt tgttttctat ttgtgtggtc ttcgatcttg tgtgcacatg
     4981 ttcaatttat taaagaaaag aaacggaaca ctacacaaag atataaagat cttgctttgt
     5041 tggtgttttt tacttcaatt gctttcagct tgttattcga aagcacgaca cgctttattc
     5101 cttttttatt ggttatctgg tttgtattct taaattttgt atataaaaga ataagcaata
     5161 gaaatatcag ttaggcccag atacattttg caaaacgaat tatttcagac ccccctgaaa
     5221 taatcgatac gcctgaaaaa acttccctgc gtataataac agtatcaatt aaacaggagg
     5281 ggataggatg aactggtaca gattgacggt agaagatgta ttgaaaaact atcaaacatc
     5341 aacagaaggc ttgtcgcaag aagaacgaca gaaacgcctg actgatcaag gatataatga
     5401 aataaaagca aggcagcaga caaaacgatg gaaaaaaata gcaaagcatt ttactgatct
     5461 gctgatgatc gtgttgatca ttgcctcttt tttgaaattt gcttctagtg aatatattga
     5521 aggaagtatt attttattcg tagtgatcgt caatggactc gttagttact ggcaagaaag
     5581 aaaagcggaa gaatcattga atggtttgaa gcagctgatg ggacaagaag cgatagtact
     5641 atcaaacggg atgcaagaaa aaatccctgc gaaagaactg gtaaaaggag atgtcattgt
     5701 cttacgtgcc ggagatgtcg ttccagcaga tatccgatta atcgaagcac atgatttatt
     5761 agtggaagaa tcgattttga cgggagaatc agaagcatct gaaaaaagtc atgccgtttt
     5821 gacagaagaa gaatcgatcg gcgaccagaa aaatatgggt ttttcaggaa cgcttgtaca
     5881 atctggttct gcagtcggtg tcgttgtaga aacaggaaac acgacagaaa tcggtaaaat
     5941 caatcaagca ttgcaatcgg tcgaacaaca aacgacgcct ctgattaaaa aaatcaaccg
     6001 attaaacaaa caaatttttc aaggcattct ttgtttgagc ctattcttag ttatttttac
     6061 gacatttcga tatggtatgg attggcatat ccttctttcg gctacgattg ctttggtggt
     6121 gtcaatggta cccgaaggat tgccagctgt tttgaccatg attctatccg ttggagtaca
     6181 tgaaatggca aaagaaaagg cgatcatcaa aggacttcct tcggtagaaa cattaggttc
     6241 tatgacagtg atctgttcag ataagacagg aacattgaca aaaaatgaaa tgacagttgt
     6301 ggatgtagct gcaaaagaag aagcctgcgt gttgtccatt atgaaaaact gtcaggaatt
     6361 aaaaacaaaa gaagaacaga aaacagaaaa tttaagtggc aatccgacag aagttgcgct
     6421 gattcgacac acagaaacag ccaatctacc tttgaaaaaa atagaagcaa agattccgtt
     6481 cagttctgaa tacaaatata tggcaacgat gcatgcggag gaaaaaggag cggtgattta
     6541 cgtcaaaggt gcaccagaag tattatttgc taaatcaact ctttcttctg cagaacaaga
     6601 agcatggagt cagacagctg cagaatttgc gagaaaaggt cagcgtgttt taggatttgc
     6661 ttataaaaaa gtagattcga aacaggaact cacgcatgag actttaaccc aattaacttt
     6721 tgcagggatc gccggattga tcgatccgcc aaaagaaagt gcagtcaaag ctgtaaagga
     6781 atgccaacaa gcagggatct cagtgaaaat gattacaggg gatcacaaag acacagcaaa
     6841 agcgattgcg gaacaaattg gcttaaaaca tactgccaaa gttttagaag ggatcgattt
     6901 ggatcttatg tctgatgaag agttgattca gcaagtgcca atagtcgatg tctttgctcg
     6961 aacgacacca gaacataaac tgcgaattgt taaagctttg cagaaaaatg gagaaatcgt
     7021 cggaatgacc ggagacggcg taaatgatgc acctgctttg aaaagatccg atgtaggaat
     7081 tgctatggga atcaaaggta gtgaagtaag caaacaggca gctgatatgg ttttaggaga
     7141 tgataatttc catacgattg caaaagccgt aaaagagggc cggcggattt tggataattt
     7201 gcaaaaaacc atcaatttct tcttgccaac tgcattagct caaggattga tcttgatttg
     7261 ggcacttatg ctgaatcgcc cgctaccttt gtccccagta cagattttat gggtcaatat
     7321 ggtaacgaca atcactttat cttatgcact agggttcgag tcgccagatc cagaaatcat
     7381 gaagcgccca ccaagagatc caaagcaagg aatcctatct ggttatcatt tgtttcggat
     7441 catctatgtc tccctactga tcatggttcc tgcctattta ttggctatgc agtttgaagg
     7501 gcaaagctta cagcaaacca ttcttctgca aaatatcgtc ttagctcaag ctgtttacat
     7561 gatcaattgc agagagttgt ccaagccgtc attgaaccaa ggtcttttgc aaaacaaagc
     7621 gctttttctt tcattaggta tccttgcttt attgcaaggg atggttattt ttctaccagt
     7681 cggccagcag ctgatcggta cagtaagttt aacgatgaca caacagttat tgattggagc
     7741 aaatgtgatt cttctattct tagttgtaga aatagaaaaa gggatcatga acaaactgtt
     7801 tcgcaaaaaa gaaaaaacag ctagagaata cagtcgctag ccgttactat aaaaaagttt
     7861 tgaaagtgag ttcagtcaaa cgattgagct catttttcgt tggaatagaa gaaatgtata
     7921 caatagggat atctagagaa tctagaggga aattggaaat caagatatct gttttttcaa
     7981 aagaaatagt ggaaagatcg gttagttcga taggtaagaa gcagacacgt tttcctaagt
     8041 aatcttttaa ttcctgctgc aagaacgcct tccatgaagg ttctccttgg aaaataaaat
     8101 agacattgaa caaattcggt ttgatagaaa aaatcgtttg ttggatcagt agagacaaga
     8161 gttggagaaa gtaatttggt tcattgattt ctatttcttt ttttagatcg ttcagtaaat
     8221 tctctttctg ttgatacaag tgtgcatatt gctgatcata tgtttctaaa aaacgatggt
     8281 attgacggtc caatgcttca atcaatatcg gcgattcatg atattttaat atgaggagag
     8341 ccaagttatc agtcagagat gtttttttta gttgatcaag agacagctct gaagctagtt
     8401 cctctacaaa cgtttcgagg acctctaaaa atggagaatg atgcaggcag cgagcggttt
     8461 cttgtccata gttattgttg taattccaac tttccagaaa gcagaaagaa gcaaaagaaa
     8521 gctcagtgtc tttaagataa ataccttccg ttaaatacaa ttttttcaat gcagagtcat
     8581 acagggagga gagtgtgctg ttggtatgga tagaagaatc taatcggcaa ttcgctttta
     8641 tacggatcgt attgataaag aaccaacatg ttccgaaaat ctcttctgtt tcaaccgcta
     8701 gttcagtttg actgaggttt cttaaaaatt gaaaataatg ggattcatgg atcgaataat
     8761 cctcgaaaaa atagttgtta tgagtgaaag gaatcagcaa tcgatgataa aaagtacgaa
     8821 tcgcacgctc ttttccaaga atacaagctg tatatttcgt caaatcgatc tgcaagtcgt
     8881 aacttgctaa acgctgattg attttagtca catgtctttt caatgtccca taagaaagcc
     8941 catggtcggc tagaaaatca gggacagcaa ctttttttgt aaaaagcaga tttttcagca
     9001 gttcgacact gactgattgt ttgaaaaaaa tctcccaaag gtcatttgtt aataaatttt
     9061 gctggttttg caaactgatt cctgcttgat cgctgatgat gtcccagcct tcgggcagct
     9121 gagagcgaat agattgaagg tcagaaaaaa tcgttcgttc ggttgtccct atgatttcag
     9181 ctaattcatt tacggttatc tttgtatgat tcagcaattg ttctactaaa gaaacatgac
     9241 gtcgaatatc tttttcggta atgatttttt tcattagtga atacatcaat cagactccct
     9301 tctattctaa gaatacggag aaaataaacg gtggtcaagt ttcaactgat ttttcaatca
     9361 ttgtttattt ttctggagca tttactgatg ataagaattt catcatgaac ttgaaaaatg
     9421 ataccactaa aatcaaaaaa taggtgggca aacaataata aacattttaa tagtagatgg
     9481 tagcaagacc aacacaggcg gattgaacat ttaagagttg ataaaaaaga aaaatatcga
     9541 agtatttgat tagcaactac ttgtattcat ttttctattc ttacgtggta tctttgaata
     9601 aagcgaatca ataagaaaga gatggaagca gatgctaacg aaaaaagaac taatcgatca
     9661 catcaatgaa acatacaatt tgtctccaga atatattttt aaaaaatttc ctgattattg
     9721 cgtattcaga catcaaaaaa gcaaaaaatg gtttggggtc ctgatgtctg ttccagaaga
     9781 aaaaatctat gatgatgcaa gtgaccatag gatcatcaat attcttgatt tgaaggtcga
     9841 tcctgaactt ggcggaattt tgcgaggaaa agaggggtat tttccagcct atcacatgaa
     9901 taaagaacat tggatctcta ttgatctacg taaaatagaa aagctagatg aactagcaga
     9961 tttgattgat ggaagtttta aatcaacgca atgaatgtct aggacaatag agaagaggtt
    10021 gtgacagaaa tgtttagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat
    10081 ttttgggaaa tttcggctta ttttctaaga aattacttct ggaacaccgt ttgttcgaaa
    10141 aaagagctgc gacaagaatt ttgtcccagc tctttttttt ttattttcct aaataaaatt
    10201 cccgcatcgt ttttgcaatc attttgatca aaggatgatc ttctgtgaag ctagaagata
    10261 aataaattgt tcgtgtcaag ggcaaatctt tgatttcccg caagacgaaa ggatcatttt
    10321 tatcgtaaga ccaagaaact tttgggaaaa agctaacagc taatccttgc ttgacaatag
    10381 aacgtaatgt cgcaggatca tctgtcgtcg aactgatcgt caattcgata cttttcgctt
    10441 caagtgctgc atccagtgta tgacggaagg cgttgctctt tgacagcatc aagatggggg
    10501 tgcgttggat atcttctaat tgcagatgtt cttttttagc aagaggatgt ttcttaggaa
    10561 tggcgacata aacaggttct ttcatgatag gaatattggt catgccaggc ttcatctccg
    10621 aagtgatgta cagtacagga tcggtaatat catctggtaa atgatgttgc atgatcgtta
    10681 attggatatc aggatgcttt tccataaaaa ttcggaccaa gttcggaata gaaacagagg
    10741 ctacctctaa atacaatatg acggaagtaa cgatcttctc attgaccgaa gacaataatt
    10801 tgattccttc ttctaatgtg tctaatgtct tgactgcata tggataaaat tcttctgcat
    10861 aaacggtcgt ctcaatgttt ctgccgctat ggtaaaacaa agggacacct aattcgtttt
    10921 ccaaatgttt gatcgcacgg ctcaaggcag gttgagaaat aaataattct ttggctgctt
    10981 gggtaacatt ttttgtttcg aatacttttt taaaatactg cagatgaagc aatttcataa
    11041 ttatccatcc catccatacc tttttgttat ggttttttct ttttttggca ttttttatta
    11101 tagcagaaat tgactaagat agcagtggaa aaagtaagtg ctgaggaggc gaggctcatg
    11161 agtacaggtt tcttatttcc tggacaaggc agtcagaagt tagggatgtt agcggatatc
    11221 cctgaaatgt acatggaacg tttagaaaat gtgacggggt atcggttagt tgataaagaa
    11281 gaaaactatc aagatacggt ttttatccag ctagcattac tgacaaaagc agtattttat
    11341 ttagatgaaa tggaaaaaaa cggtattcag cctgatattg cagcaggaca ttcgattggt
    11401 gctttttgtg cagctgttgc ttgtcgatct ttgagatttg agaatgcagc ggaactagtc
    11461 tatcaccgcg cagtcttaat gaaagaagct tatccaagcg gttatgcaat gggcgtagta
    11521 gtaggtgtga cgagaagcga agctgaggaa attgtagcaa aaacatttga tcctgaccat
    11581 ccagtttatc tgtcaaatga aaattgtccg atgcaacata cgttatctgg gagtatcgaa
    11641 ggtttatccg ttacgttggc agaagccaaa cggcaccaag cgcaaacagc gaagctgctg
    11701 aaagtccctg tgccatctca ctgtctcttg atgaatgata cggtcaagaa attcaagccg
    11761 tatatgcagt ctgtccatct atctgatgct agatgtttgt atataaaaaa tacggatgga
    11821 agagccactc aggacgcaga agagattcgt aaagatttgt tggaaaacct agctttccct
    11881 gtgcagtgga accaaatgat ggacttggca aaagaattag gaatggatct gacgattgaa
    11941 tttccccccg gcaatacttt gacaaagctg attcatacaa aatttggtgt agataaaggg
    12001 atccgtacga tcaatctaga ccaacatggt attgatgacg ccacgtttct atatcaaaaa
    12061 tggagatgac taattatgga aaaaccaaac cgcaactggg cttctaaacg cgaaaaaaaa
    12121 gcgcagcgat tggaaaaaat cagcaacttg ctcaatggca agtatgtaga aactgaaaaa
    12181 atcgtagaag taatggaaaa actgattgct cccggcgaca aagttgtatt agaaggcgac
    12241 aatcaaaaac aagcaagttt tctttcagaa agtcttgaaa aagtagatgc agaaaaagtg
    12301 aatgatctgc atttgatcat gtctagtatc tcacgaccag aacatttgaa tatttttgaa
    12361 aaaggaatcg caagcaagat cgatttctct tatgctggtg cacaaagctt gcgtgtcgcc
    12421 caaatgattg aagacggcac aatgaaatta ggtgatatcc atacttattt ggaactttat
    12481 ggacgcttat tcatcgattt gattccaaat gttgttttag tagctgcaga taaagcagat
    12541 aaagacggca acttgtacac aggtttcaat acggaagaaa caccaacaat catcgaagct
    12601 gcagctttca aagatggtat cgtgatcgtt caagttaacg aagttgtcga ttctttacca
    12661 cgtgtagata tccctggtga ctgggtagac gttgtcgtga aagcagataa accttatcaa
    12721 ttagaagctt tattcacacg agatcctcaa aacatcacag aattgcaaat tctaatggca
    12781 atgatggcaa tcaaaggaat ctacgcagaa cacggcgtac aatctttgaa tcacggtatt
    12841 ggattcaata cagcagctat cgaattgtta ctgccaactt atggtgaatc actagggtta
    12901 aaagggaaaa tcgcgaagaa ctgggtattg aacccacatc caacgatgat tccagccatc
    12961 gaatcaggtt gggttgaaag catccatagt tttggtggcg aagttggtat ggaaaaatat
    13021 attgctgctc gcccagatgt cttttttgtc ggtaaagacg gcacgatgcg cagtaaccga
    13081 acattatcac aagctgcagg acaatatgcc atcgatatgt ttatcggttc aaccttgcaa
    13141 ttagattacg aaggaaactc ttctaccgta acaaaaggac gtttatctgg ttttggcggt
    13201 gcgcctaaca tgggacataa tcctggcggt cgtcgtcatt ctacaccagc atggcaatca
    13261 cttcgcgaca atgatgatcc attagggaaa ggccaaaaat tagttgttca aatggttgaa
    13321 acatatggtt caaataaaaa accagtcttt gtcgaagaat tagatgcact agctgttcaa
    13381 aaacaagcag gcttagccaa tgcaatgcac cagtgatgat ttatagcgaa gatactacac
    13441 atatcgtgac agaagaagga attgcttatt tgtacaaagc aaaaacaaaa gaagaacgac
    13501 aagcagcaat tgcagcaatt ggcggtgtta cacctcttgg aatgactagc acgaaagaat
    13561 cactagatag cttgcgtgca gccggcatcg tgaaattacc agaagatcta ggcatcaaac
    13621 gtagtgaagc gaaacgttca ttattagctg cacaaaccat cgatgatttg atcgaatggt
    13681 cagatggcct gtatgaacca ccaatgaaat tcagaacttg gtcataaaat tggcgagaaa
    13741 aaccttttgt cgccatttac ccagatggaa gaagaaagac gaataagcag aacgtgtatg
    13801 ctctgtttta caaaaagggg agagatccat ggaacaatta gcttataaat atccaacgaa
    13861 acagccgatc aataagaaaa tccatgtagg agtggtcggt tcaggagatt tggaaatctt
    13921 gatgtttcca acagaaaaaa cagaatctac tgtaaaaatt tgtacaggaa gcgatggctt
    13981 cggcgaagta tggaacaatg tattaacgag attttttgat cgttatccaa tttcagcaga
    14041 tatcgtgatc aatgacttcg gtgctacacc aggagttgtg tacctacgat tgacgcaagc
    14101 aatggaggaa ctttcagatg aaaaatagtt ttgtagaatt aaacgccaga gaacgagtga
    14161 acgctctact tgacaatgga cgtgaattga ttgatccatt cgatcaaatc atcgcgccaa
    14221 gtttaccagc acaaggaatc gtccctgaaa gtgatgacgg gatcgttgtc agccgtggca
    14281 cgatcaatgg caaacaagca gtagtaatcg ctatggaagg aaaattccaa ggtggcggta
    14341 tcggtgaagt ttccggtgca aaaatcgttg ctgcacttga acatgtgtta gaagaaaata
    14401 aaaaaggaaa tgagatcttt ccagtcattg tactagatac aggtggtgtt cgtttgcaag
    14461 aagcaaacta cggactgtta tctatttcag aaatcggaaa tgtaatcatt gctctaaaac
    14521 aatatgtacc agtcatcggt ttgattccag gacgtgtagg atcatttggc ggtatgtcga
    14581 ttacttcagc attaatgagt tatttaatcg caacaaaaaa agcgagagtc ggtttgaatg
    14641 gaccagaagt tattgaacaa gaagcaggcg ttcgagaatt tgattctagt gacaaagact
    14701 tgatttggaa tacattagga tctcgtcaac gggtagaatc aaaaatcatt gatgaattag
    14761 tgactgatag tgtagaagca atcaaagctg ctgtcatcgc agcaatggac gaacgaaaag
    14821 acacgcatcg cagtgaaagc tcagcatttt atttatcatt acttgattct ttagatcttt
    14881 caaaaccaat ggccattgac gattacaatg aagcaattgc taaccatcaa gcaaaatcaa
    14941 ttgacttacc agaagtagca gaaggaaatg aaccagctgc agcaagtgtt ggttacgatt
    15001 ggtttgcggc actaactggc atgaaaaatc caacttcttc tattagtact gtgtattatg
    15061 gtaaatcaag taaattcata gaagaagcca tcgtgacatc aatcgtacct gatcctaaaa
    15121 acccgatgta ccgtgtacga aacggtgaag ttggtttagt tgaaggtttc cgcatgggac
    15181 aaattttgga tcacgtttac gaagaagata aagacaaaaa agtgaaacgg ccaatcatcg
    15241 ttgtgatcga tgtaccaagc caagcatacg gctataacga agaattgatc ggtatccatg
    15301 tggctttggc taatagtgct gctgcttatg caaaactccg tcaagctgga catccagtta
    15361 ttggattgat tgcaggaaat gctatttcag gtgcattttt agcacatgga ttacaatcaa
    15421 gccgcttgat tgctttaaac aataaagaaa ttaccgtcca agcgatgtct aaagaaagtg
    15481 ctgcacgtgt gacaaaacgt tcggttgctg aaatcgaagc agctgctgaa aaagttccag
    15541 ctatcgctta cgatatcaat aattatacaa aactaggagc gatctatcat ttcctagaag
    15601 atatcaccga ccaagctgct tcagaacaaa atgtcagcaa agtcatcgaa gcaatcaatg
    15661 aagccattca agatgtccgc aatacaaatg acacgatgct gacaaatcga tacacaaacg
    15721 aaacagccaa aacattcgga cgtgtcgaaa caaataaagt ccgtgccaaa atggacgaag
    15781 aatgggatgc ataaaaaatg attcaagcac atgatattgt cctttttgat aaacatgaaa
    15841 tagaagggtt caccttgcct gaatgggtcg ttctaactga aaagatgttc ggaacagttc
    15901 gtcgtgcgtc gacggatcac gcagaaatac taccgattgg tttacgtgga cctgcccgca
    15961 atcagagatg gtcaacagaa atctcaaaac atgccgtcaa acaagtcatt catccgtggg
    16021 acatcatcga acaagaatcg tatagacaag agaaaatcat ggattatcct gtttatcagc
    16081 aatttcatgc tgctagaaac atattatcag gatgtaaatg gggaatcggc ggcagcttag
    16141 gatttgaatt atcgactggg attccggcag taaaagaaac cagtgatttt gatttgctgc
    16201 tgtacgcaaa ttcaccgatt gaattgccaa ttcaggtaat tcaatcgcat cctgcttttt
    16261 ttgagcaatt cgatacccaa gtagtgactc ggcaaggcgg tttttcacta aaagagtatt
    16321 tgagagagcc agagaaaaaa attctattga aaacagtcga aggacctaaa ttaaccaaag
    16381 aactttggta ataaagaaaa agagagagaa agagatgttt gaaaacaaaa aaatcaaaat
    16441 caacgtaagt gacttccaga aagtatcagc tgtaacggct gttatgatgc aaacgatatt
    16501 gagctttgct ttggcaaaca gccacagcgc aacaaataca gcttttcttg gtacctttta
    16561 cgtcatcgtt aaatacacag caccaatgtt tatttttgca attgtttata atatggtaaa
    16621 aacaagtcag catttgtctt attgggaatt tttgaaagaa aagttttttg aactagtcgt
    16681 tccgtatctt ctatggacga cggcttatct ttggctattt ccagcagtcc aacaacaaac
    16741 tccgtatacg gatactgcaa gctttttact gaaatatgtc acaggtgatg gagcgcctca
    16801 tttatggtat accgttatga tgctgcagat ccaattgttc atgccgtttt ttgtttggct
    16861 cggttacaaa gtatttgcta ataaaaaata tgtatggcca gtattgattg gttcaacgat
    16921 tttatatgtc gcatggtatc tgttctatca aacacaagta ttgactgggc cttatcatga
    16981 cagctggtac ttattagatc ggtttgttgc tagtttcatg atttatgggg tagccgcatt
    17041 tgtttatcac gaaaaagtct atcaatattt ggacagagta cgttatcttt tcttacctgt
    17101 tgccttagtt atcgcgttct tttctgtccg ttcattgctc gcacatccag gagacttgag
    17161 ctttgcgaat gcaccttact tgaatacgat ccaaagttta tacagtttag tcatcatctt
    17221 tgctgtcttc ataggcgctt ccaaaatgat tgtaaacgat tcgccaaaat tgccattgtt
    17281 caaatggttg tctgtttatg cttatcgtac gtatttagcc aatgtatttg ttttccaagt
    17341 actgttatta ttgtttaaag atagttggct gcagttacct tcaggagtca tgattcttgt
    17401 agcttatcta atgacggcaa gttgtgcatt tgctttaagc tggttacttc atatcatttg
    17461 ggtagctatc aaaaaaggtt tcagcaagta atagtaggaa gaaggagtga gtgtcatgat
    17521 aaatgacgaa cgaaaaaaag cagtgactca gcaactcgct gcgttggtag aggaagcatt
    17581 gatcgcagaa gtaacattaa gtccaaaacc tggattagtg gatgcactgg atgcgggtgc
    17641 acattccgat atggactatg ctttatttct taagtctgct aagacgctaa ctcctttttt
    17701 tgaagaaatg gctcaaatag catggcatca tgctattgat caggagttaa gagaaagaat
    17761 tgctgagatt gggcgaaaag cggaaaaggc aatgctgatt gctacaaatg gcatcaatac
    17821 ccataaagga gcgatttggg caatggggct attgatcagt gtcattgcac aaaaaatcag
    17881 tatggaacag cctttttctt tatcgaaaat acttgatgat gtcagcaagc ttgctgcttt
    17941 tcctgataca aaatatcgtc ataaaaaaac cactcatggt catagggtga agcaaaaata
    18001 tggcgtaaat ggtgcatatg aagaagcgat attagggtat ccgcatatca ggcttgcaat
    18061 tcaagcagct aaggagctag ataatgattc atatgtacag aaacaaatgc atatgttgtt
    18121 agttttgatg agttcgctag atgacacatg cgtattgcat cgcagtgatc agaaaacgtt
    18181 agtggaaatg caaaaacttg ctcatgctgc tagtcaaagt gctttaccaa atcgtccatt
    18241 tacagcactg ttgcactttt gtcaggaaaa ccaaatctct cctggaggaa gcgcagatct
    18301 cttgtctgca agcttgttca tgctgaagat agaggaatta tggactgccg acacatacat
    18361 ttgtccgatg ttactcaatg actaaataag acaaaattgc taaaaaaaca gtttatgcat
    18421 ctggttattg actcttcctt ttagcaaggc tatcataaaa atagtttttt gtaataaaag
    18481 ataacgagga agacaatgaa aaaaatcaat tatttagcaa tcattttgtt agtgggccta
    18541 tgtttgtttg ctctgccgac ccaaggattc gctgtcgcgg gtggtcatgg tggaggtaca
    18601 tctagcggtc gaagttcggg cagaggttca tttggtggtc gttcatatgg gggcagttat
    18661 tttggcagtc gttcttacgg cagagattct tatgggtatt atggtccagg tacaggtcct
    18721 tctagttatg agcaaacggg atttcttgca ttagctgttt tgtcagtagg aggggtttct
    18781 gctttttcta tcgtcaagaa taaaagacaa aagaaaaggc tgaaagcgga gatattgtcc
    18841 cgcatgtcag gaacggctaa agaaaagaaa gcacagtttc tggaaatcca aaagatattc
    18901 atgaagatcc aatccgcttg ggaagaagaa actcctgaaa aagccagaag ctgttataca
    18961 gacaagctgt ttgccgaaca tcagaaagtt ctgcaaaaaa acaaagcaga cggtgtaaga
    19021 aatcatacta aaaaaataac tattcaggaa gtaggaaatt atcgacagat ccacgaaaat
    19081 agttttgcca tccgaattga tttcagatgc tgtgattatt cagtcgatcg aaaagatggg
    19141 cgattgatct caggaagtaa gacacaacga cagtattttt ctcagatatg gtattttgat
    19201 ttttctgaga aagaaaaaag atggcaagcc gattttattc agccgattat gctggattga
    19261 caggaaatat ttatgaacgg agttgtgaca aaagccttgt cgcagctcct ttttctgaat
    19321 aaacggtgtg ccagagatag cctatcggca ataaggcacg attttgtaaa atatgggaag
    19381 cgatttttca aaatcgcttc ttatcgctca aggctgaaca tctctgtcac aacctctcca
    19441 ctaaatggtg tttaaaagct tgcatctgcg ataataagaa caaccaaacg aaaaatagga
    19501 agcactattt tagtttagtt gttcttatta cttgatgcag gacagctttt tcacaacctc
    19561 ttttttgtat agatgggcaa aatttttgta ataataggcg tatgtctttt tcgttgagga
    19621 gatgaaaaag catatggctt attttttgtt tgctttgctt actttctttc cacttcaagt
    19681 ggcagaaata tctacttttt attatgtaag cggaaccata tacttgaaat cagaaggaga
    19741 tcatttctat gaaaacaaga acaattgaaa tacttcacag aatcatgaca gcccaatatc
    19801 cagtaaaagc aaaacatcta gcaagtgagt tcaatgtcag tcagcggacg atccgtcagg
    19861 aagttcttga agcgaatatc tggttgagca gtaaaaagct tccggagatc cgtacaattc
    19921 gcaacaaagg ttttttgttg aagctggaaa aggaaaagca agctcgatta gaaactgaaa
    19981 taaaagatgt gaaagacggc tttttgaatc ggcgcgaacg tatttttgat ctagttttag
    20041 ccgttgctta cagccgaatc ccgatccatc taaacaaaaa agaagaagaa tttcaaatct
    20101 ctaaaagtac attagatgaa gatatgagac ggttacgagc aacactggtc agttatggga
    20161 tagagatcgt cagttatgga aagcaaggac tggtttataa aggttcagaa cgatctatcc
    20221 gtacgatgat ctatgatttt atcaatcaga atctgggaat gaccgacttc tttatgaatc
    20281 aagatgcaca attaacaccg gctcaaaaaa tctttctcca ttattttcct ttggtggaaa
    20341 tcaaaaaaat acaggagatc tattccaata agttgttgaa acaagaagat gatatttata
    20401 aaaaacaaat cctgcttttt acattgatct ggatccagcg aatcaggaaa catgaattta
    20461 tctctgccat taattggaaa aatacagaaa taaaagacag tagcttttct gcatttattg
    20521 accacatgat cgaggaattt caattggaca atgtcccgca agtagaaaga aattatcttc
    20581 gtttcactat tgagacgttc aatgctagag atatctccaa ttcaatcgag tgggtacaag
    20641 ctcaattact aacgatccaa ttgatccaat ttgtagagaa cgagacacac gtacctttcc
    20701 atctaaagga agaagcttta ttcgaaaatc tctataagca catggctgca ctgattgtaa
    20761 gaatcaagaa tcatatccaa gtcatgaatc cattgaaaga aaacatccga acaaattatt
    20821 tcccaatcta ccgcgcagtc actcattttg ttcctacgat cgaagaagta attggtggca
    20881 agatactgga ggacgagata gcttttcttg tcatccactt ttcgacgatc gccagtgtaa
    20941 tcaagcgaga cttgaattat gtttataaat cagtcgttgt ctgtaaccat gggatggcta
    21001 ctggaaatct gttagcagag aatctgaaag aaaagtttcc tcagatcgag attactgcag
    21061 ttttaagttc aaaagaaatc gaattagtag ataaattgga tgtcaatctg gttttcagca
    21121 cctttcaatt gaattatcga aataagccgc ttttagttgt cgatccgatt ttaactgaga
    21181 cgaatatctt ggttatagaa gactttttga aaagaaatga agaactacag cggttagagc
    21241 ctaacagtca tgaatcgacg gagttattca ctcaaataat agatgtcatg aaagaaagtg
    21301 gtggccgagt agatcgaatc atctatggca agttagaaaa attattcgaa acaaacaatt
    21361 tagaaatcaa caaaagggag atccagccta tgttaaaaga tattttgacg gacagcaata
    21421 tcatgatcaa agaaaaagcc actacttggg aggaatcgat tgaactagct gctagaccat
    21481 tggttaaaga aaacattgtc gaagagcgct atgtcgaagc gatgattcgt gcagtgaatg
    21541 attacggacc gtatatcgtg attggcaaac acatggcact agcccatgcc cgaccagaag
    21601 atggggtcaa taaattagga gtcagcgtag ctacgattga gcaaccaatc gatttcggta
    21661 atcctgaaat ggatccagta aagattattt tttgtttggc agcagtagac tcctactcgc
    21721 atctgaacat catgaaagaa ctaattgaat tgatcaacaa cgaagagaag ttggatcgat
    21781 tgattgaatg cacaaatatc gaagatttca aacaactgct tttttcagta gttgagaaat
    21841 aataaatgat acgggggaga acaaagatga acaaattgaa cattttattc gtatgcggtg
    21901 caggtttagg aagtagtttt gcagcacaga tgtctgctga ggatgttttg aacaaattag
    21961 gagtagaagc caaactggac cattgtgata tttcttccgc agtttccatg aaaccagatg
    22021 tgatcatcac agcagaaaat tttcgttcgc agttcgaaaa gttcgcagtt gatcctaaaa
    22081 cggcgatgat ctatttgaaa aatattgttt caaaaaatga aattgaagaa aaaatcaccc
    22141 cagttctgca agataaggga gttatctaaa tacaaaaaga aaagagggta aaaa
//
LOCUS       DAYYAJ010000039        21637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000039 DAYYAJ010000000
VERSION     DAYYAJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.039"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1090
                     /locus_tag="ACHATZ_001252"
     CDS             125..1090
                     /locus_tag="ACHATZ_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8403794.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1147..1377)
                     /locus_tag="ACHATZ_001253"
     CDS             complement(1147..1377)
                     /locus_tag="ACHATZ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8403795.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1463..2350)
                     /locus_tag="ACHATZ_001254"
     CDS             complement(1463..2350)
                     /locus_tag="ACHATZ_001254"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8403796.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2439..3662)
                     /locus_tag="ACHATZ_001255"
     CDS             complement(2439..3662)
                     /locus_tag="ACHATZ_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8403797.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3679..4725)
                     /locus_tag="ACHATZ_001256"
     CDS             complement(3679..4725)
                     /locus_tag="ACHATZ_001256"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8403798.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4889..6616)
                     /gene="ptsP"
                     /locus_tag="ACHATZ_001257"
     CDS             complement(4889..6616)
                     /gene="ptsP"
                     /locus_tag="ACHATZ_001257"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8403799.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6616..6882)
                     /locus_tag="ACHATZ_001258"
     CDS             complement(6616..6882)
                     /locus_tag="ACHATZ_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8403800.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7361..9595
                     /locus_tag="ACHATZ_001259"
     CDS             7361..9595
                     /locus_tag="ACHATZ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8403801.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9743..10063
                     /locus_tag="ACHATZ_001260"
     CDS             9743..10063
                     /locus_tag="ACHATZ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8403802.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10268..10492)
                     /locus_tag="ACHATZ_001261"
     CDS             complement(10268..10492)
                     /locus_tag="ACHATZ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8403803.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10690..12270)
                     /locus_tag="ACHATZ_001262"
     CDS             complement(10690..12270)
                     /locus_tag="ACHATZ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8403804.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12671..14047)
                     /locus_tag="ACHATZ_001263"
     CDS             complement(12671..14047)
                     /locus_tag="ACHATZ_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8403805.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14175..15263)
                     /locus_tag="ACHATZ_001264"
     CDS             complement(14175..15263)
                     /locus_tag="ACHATZ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8403806.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15435..15869
                     /locus_tag="ACHATZ_001265"
     CDS             15435..15869
                     /locus_tag="ACHATZ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8403807.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15947..16480)
                     /locus_tag="ACHATZ_001266"
     CDS             complement(15947..16480)
                     /locus_tag="ACHATZ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8403808.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16574..17752)
                     /gene="mutY"
                     /locus_tag="ACHATZ_001267"
     CDS             complement(16574..17752)
                     /gene="mutY"
                     /locus_tag="ACHATZ_001267"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8403809.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17745..18542)
                     /gene="recX"
                     /locus_tag="ACHATZ_001268"
     CDS             complement(17745..18542)
                     /gene="recX"
                     /locus_tag="ACHATZ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8403810.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18649..20031
                     /gene="rlmD"
                     /locus_tag="ACHATZ_001269"
     CDS             18649..20031
                     /gene="rlmD"
                     /locus_tag="ACHATZ_001269"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8403811.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20205..20783)
                     /locus_tag="ACHATZ_001270"
     CDS             complement(20205..20783)
                     /locus_tag="ACHATZ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8403812.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21105..21575
                     /locus_tag="ACHATZ_001271"
     CDS             21105..21575
                     /locus_tag="ACHATZ_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8403813.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         6458 a         4356 c         3750 g         7073 t
ORIGIN      
        1 tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat agaattccct
       61 aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg taaaaggaga
      121 tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct atcttatgaa
      181 agaccgagat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa atagagtaag
      241 cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga ttcatcatat
      301 aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc tcgagtgtca
      361 attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca gtaattataa
      421 ttatgtacat attaccaata agggatttgt attaacagat actcctacgg ctcgaacata
      481 cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg aagaaactta
      541 tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagtccagc ttcctgacct
      601 acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa
      661 taaaaagcta ctgacttact tgaaaaaaag acaacaacat ttcatctatc agttgaattt
      721 cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta ttagacggat
      781 ccaggaacaa aagcagtttt taagaacacc atcgctcacc ttcagcatgc cattagcagc
      841 agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag aaacaatgat
      901 tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc atttatcttt
      961 gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca gttaccgcaa
     1021 aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag ctgaaaagca
     1081 actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg tcaattatcc
     1141 taaaatttat tggattaagt catagatttc catcgctacg atatcgatat tatcaaattc
     1201 gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt tatcataagt
     1261 tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt cattatctgc
     1321 tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat gtgtcatcgg
     1381 aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac ttgcattgat
     1441 tttctctaaa aaaactcgtt ttttatgatt ttttttctgg tatttttcct tcaggccacc
     1501 aaagtttgat caaagcttgg gttccgtcat tctcaaaacc tttatctgcc aactcttcat
     1561 atagtttcaa tgcttgggta gttgctggca atacaagatc cattttttct gcttcttcta
     1621 atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt
     1681 ttaaaatacg aggagaataa ttgcttaaag accagttagc tgcacttcca cctgaaagcg
     1741 tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc atagacaagc atttccgtca
     1801 ttcctgtcat cgtacctgca atcatgatct gattagccat ttttgtatgc tgacctttcc
     1861 cagcagaacc atgcaatgta aatgtcgtac caaattcttt gaataaaggc agtacttgat
     1921 cataaacggc ttcttctcca ccgaccatga tcgtcagtgt accattcttt gctcctaagt
     1981 caccaccgga taccggtgca tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa
     2041 ttttttctgc caggcttggt gtacttgtag tcaaatcaac taaaatatgt cctgagatat
     2101 ctgcagaaaa aatccccgac tgtccatagt agatttcttc tacatctttg gggtaaccta
     2161 ccattgtaaa gagaatatta ctttgttctg ctaccgcttt tggtgtatct gcccagatag
     2221 ctccttctgt tactaaagga tcagcttttg cttttgtccg attatagaca gctacttcgt
     2281 gtcctgcttc tagtagatgg cgtgcgacag cacttcccat gacgcctgtt ccgataaaac
     2341 caagtttcat tttttcgacc tcctaaaaat caaaaatcct tgcttctatg ctagtatacg
     2401 ctagaaacaa ggattttttc ataaaaaatg tttgaatttt atcttttaaa tgaatttaat
     2461 tttattttga ttcgttcaat cgagtcttct tcttcttttc ttcgcatctc ttcttcatga
     2521 taaacaacca tgtcttgata aaattcgatc atagcttcgc cgaaatgtgt agatgaaata
     2581 tcataaagtt tagcttctaa aacttttgga tctttaggaa cttcactgga aatataatca
     2641 ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg
     2701 aacaatcggt tcaaataagc atttccttcc acgacacaag gtgttccggc tgccatcgct
     2761 tctgtataag taagcccttg tgtttctgaa gtagaagcac tgacaaaata atctgccgct
     2821 ttataataaa gtgctacatc attgttgtcc acttctcctg tgaactgcac aaattcttct
     2881 aactgcagtt ctgagatcaa ttcctctaac tcttctacat aaggtccttt tcctacgata
     2941 actaatctgg cgttaggata agcggagatg acctgaggaa acccattaat caaggcttgg
     3001 atatttttct cataagaaat cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga
     3061 cctagtgact cccttaattt gctgatatcc tgctgagtga tatcagaacg tttgaatttt
     3121 tcaatgtcga taccagtagg aatcacgcga agcggtgcta ttacaccata gctgcgtaat
     3181 gtatcaataa cacgttcact tggacaaact acacctgtcg tatgatttgc aaataatcta
     3241 gacaagtatt ttacatggga ttgcctgatc acttttcctt taccgatata atgaaggtag
     3301 tcttcataca tcgtatggta agtatggatc agaggtattt tcaacttctt agctaccatc
     3361 ttcccaagta tccctgctcc gaattctgta tgggtatgaa taagatctag ttctagttct
     3421 ttggcaacca aataagcata ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa
     3481 ataaatggta cacttggcat tcgaatcaca tctttttcca acttatccgc attaggatcg
     3541 gtcgttgtaa aaatatagac ttcatgccca tgtctttcta attcttgttt taatgttttg
     3601 atcgatgtag caactccgct gacttgcgga aagtaggtat cggtaaaaaa gccgattttc
     3661 atgtttttcg cctccaaact agtccttgac ctgatttact ttttttaatt tgctagagac
     3721 tttttcattc agtacacttt cgtatacatt tttaagttct tcaccgattt tattgatact
     3781 tcgctctttt gctgtttgat aaccggcttg tgtcgtactc ggtagttctt gattggtaat
     3841 ctgttggatc agtcggataa attcttcgtt tgtcttcccc atatagcagt tcttgctatc
     3901 ttctaaccag ccattataca ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa
     3961 agcttctaaa acaacgatcc cttctgtctc ttcataagaa gggaaaaaga atagatctgc
     4021 acctgaatac gcaccttcta tgatctctcc acgaatgtaa ccaggaaaca ggacattttc
     4081 aggatgatct tcttttacaa ttttccggat gttccgagga atcgagtaca tgggaacatg
     4141 accaaaccaa ataaatgtgt attcgggcat cttcttagca atttctacga aatcaatgat
     4201 tcctttacgt tcgaaaaaca aaccgacaca aatgattact ttttgatccg gagacagttt
     4261 gaaatattct ctgaactttt gttctttcat tggatcaggg tgatatttct ctaagtcgat
     4321 cccattggaa atagcttgaa tcggcacacg gataccatag ctttccaaca actgttttga
     4381 ataaggtgtt ggcgtgatca gatagtccgc tttttggtac aatcctacca gatatttttt
     4441 tacgatagga gaaagctggt tcgatctgat aaaggaattt cggaaatctt cctctgtaga
     4501 atgcgcatga tagacaactt tttttccttc tcttctggct ttattgacca tattatgact
     4561 attgattccg tatgtattga tgtgcaggat gtcataatct tctttttcat ctagtgtata
     4621 agagataccg acttccgtca gagcacgttt ttgatgatca agcgcacgcc cgattccaga
     4681 ttttgcaagc atcttttcac tttcaaaata taataatacc ttcaccttac gtcccccttc
     4741 acttatatag tataacatag cctacaaact gtcactcaaa cttaagccct atttttgtct
     4801 attcttactg acaaaaacgt tttcttaaaa aagaacaaaa aaactggcgt agctctttta
     4861 caagccacac cagcttttga tacgttgttt attttacata ttcatgaaca agttcaacga
     4921 cttcttccat catatcgcat tctttcaatg cacgatcagc aagttcagcc attttcgctg
     4981 tgtccaagcg tttcattaaa ctacgcgttt taagaataga tgtcgcactc attgagaact
     5041 catctagacc catacctact agcaatggaa cagctgtttg atctccagcc atttctccac
     5101 acatacctgc ccattttcct tcagcgtgtg ctgcatcaat tacatttttg atcaaacgta
     5161 aaattgatgg gttatatggt tggtacaagt aagaaacgcg ttcgttcata cggtctgctg
     5221 ccattgtgta ttgaatcaaa tcgttcgtac ctacagagaa gaaatctact tcttttgcaa
     5281 acttgtctgc taagactgct gctgctggaa tctcgatcat gatgccgact tggatcgtat
     5341 cagatacttc cttgccttca cttatcaatt tttgtttttc ttcttcaaag atcgcttttg
     5401 ctgcgcggaa ttctttgagt gtagctacca tagggaacat gatacgtaaa ttaccatgaa
     5461 cagatgcacg aagcaatgca cgcatttgtg tacggaacat ttcgtcgcct tgttcagata
     5521 aactgatacg caatgcacga tatcctaaga atggattcat ttcatgaggt aattgcagat
     5581 atggtagttc tttatctcca ccgatgtcca ttgtacgaac aactacaggt tttccttcca
     5641 taccttctaa tacagctttg taagcttcat attggtcatc ttctgttggg aagtctggag
     5701 aatccatata caggaattca gtacggtaca gaccaactgc ttctccacca ttattatgga
     5761 caccaactag atcttttggt gttccgatgt ttgcagctaa ttcaaaatgc ttgccgtcag
     5821 ctgtgacagt ttctgcattt tttaattttt cccattctgc tttttgtgct gcgtaatctg
     5881 cacctgcttt ttcaaattca gctttttgtt catctgtagg atcgatgatt acatcgcctt
     5941 cgatcccgtt gacagccaaa atatcgcctt ctttgacttt agcagtaatt tcttttgtcc
     6001 caacgattgc tggaatttct aaagaacgtg ccatgatagc agaatgtgaa gtacgtccac
     6061 caatgtctgt tacaaacgct tttacaaaat tgcgatctaa ttgtgctgta tcacttggag
     6121 tcaagtcatg tgcaacaacg accacttctt cgttgatcat tgatggattt ggtaaagtaa
     6181 cgcctaataa gtgcgctaaa atacgtttag caacgtcacg aatatctgca gcacgctctt
     6241 gcatataagc attatcttcc attgcttcga acataccgat atacatatcc gttacttctt
     6301 ttagcgctga ttcagcattt acgctgttgt ctttaatatt ttgcttgatt tgacctacca
     6361 tttctggatc tgaaagaacc atcaaatggg catcaaatac ttgcgcttct gcttcaccta
     6421 agctttgcgc tgctttttca cgaatttgct gcaattcttc agtagattta gctaatgcag
     6481 catccaaacg agcttcttca gccgcagtat cttctactgt tgccttactg aatgttaaat
     6541 ccggttgaac tagcaggtaa gcttttgcaa cggctacacc gtcactagcg gcaatccctt
     6601 ttagcatttc aaccattatt cagataagcc ttctttcttc attgtttcaa cgattgctgc
     6661 cattgcatca gcttcgtcag caccttctgc agtaatagta acatcagaac cttgacctac
     6721 acctaaagac ataacgccca tgattgattt caagtttact gatttacctt tatactctaa
     6781 gtttacatca gagttaaatt tgcttgctgt ttgtactaat agtgtagctg gacgagcgtg
     6841 gatcccagtt tctgctacta cgtgaaattc tttcttttcc atattaatcg gtctccttta
     6901 agtgagtgtt gtttttatgt gagggttttc ttttttagta attcagagaa tcctaacaaa
     6961 tgccaccctt tcatctgtaa agattaacat tttttttcgt tagatacaac agttttcatt
     7021 caatttcaga taaaagtatt caaattattc cggattcttt ttcatccttt tcttcacatt
     7081 ttataacaaa cgtgtttatt tcctttttct tgtcgctttc tcttctcctg acgtttatca
     7141 ttctttcctt tgaccctctt tgttttcata tgaaacgaat tacttgcttc cctttttatt
     7201 tttagtataa taagagtcaa aggtcaggaa tggtcaatag agatttcacc ttttctacct
     7261 cataaatctc ttaaaaaact aggggttatt cttttacctc atgaaaaacg agatgtaaaa
     7321 tatggatgta cccatttttg tgaaaggacg tgaatgattc atgctttgtc aaaactgcgg
     7381 caagaatgag gcaacgattc acttgtatgc cactgtcaat ggtcagcgga cacaactcga
     7441 ttactgtcag agctgttatc aaaaaatcaa aaatcaacaa aatggaggtc tgactaatat
     7501 ggcccaaaat gatccatttg gatttggcag tttggacgat cttttccgtt ccatgtctcg
     7561 ccaaatgcaa cagcaaggaa attatgagca aacaccacca acacaatttg gcggaaacaa
     7621 caacttcaat ggtggtcagc cacctcaagg caatacagaa ggtttattgg gagaatacgg
     7681 aatcaatatc acagaacaag cacgacaagg tgatatcgat cctgtgatcg gacgcgatga
     7741 cgaaatcaaa cgagtcattg aaattttaaa tcgccgaacc aagaacaatc ctgtcttgat
     7801 tggagaacca ggtgttggta aaacagcagt tgttgaaggt ttagcacaaa aaatcgtaga
     7861 tggtgatgta ccgcaaaaat taatggacaa agaagtcatt cgtttagatg tcgtatcttt
     7921 agtccaaggt actggtatac gaggacaatt tgaagaacgt atgcaaaaat tgattgaaga
     7981 gatccgtcaa gcagaaaatg tgatcctatt catagatgaa gttcacgaaa tcgtcggtgc
     8041 tggttctgct ggtgatggaa atatggatgc tggtaatatt ttaaaaccag ctttagctcg
     8101 aggagaattg caaatggttg gtgctactac cttaaatgag taccgcatca tcgaaaaaga
     8161 cgctgcttta gaacgtcgta tgcaacctgt acgggtagat gaaccaactg tcgaagaaac
     8221 gatcagtatt ttgaaaggtt tgcaaaaacg ttatgaagac tatcatcatg taaaatatac
     8281 tgatgaggcc atcgaagcag ccgcaacttt atccaatcgt tacgtccaag atcgcttctt
     8341 gccggataaa gccatcgatt tgctagatga aacaggttct aagaaaaact tgacgatcca
     8401 aatcgttgat cctaaaacaa tcgagaaaaa actgcaagaa gcagaagaac aaaaagtttt
     8461 agcttctcga gaagaagatt tcgaaaaagc agcttattat cgcgatcaga tcaataaact
     8521 tcaaaaaatg aaagaacgtc agctcactga agaggaaaca cctgtgatta ccgaaaaaga
     8581 tatggaaaaa atcgttgaac aacggacagg tattccagtg ggtgaattaa aagaaaaaga
     8641 acagacacaa ctgaaaaact tagcagatga cttgaaagct catgtcatcg gacaagataa
     8701 cgctgtagat cgagtagcga aagcgattcg ccgaaaccgt gttggattaa acaaacagaa
     8761 tcgtccaatc ggctccttct tatttgttgg accaactggt gtaggtaaaa cagagcttgc
     8821 gaaacaactt gcctacgaac tattcggttc acaagattca atgattcgtt tcgatatgtc
     8881 tgaatatatg gaaaaacata gtgtctctaa attgattggt tctcctccag gttatgtcgg
     8941 atacgaagaa gctggtcaat taacagaaaa agtacggaga aatccttaca gtcttgtctt
     9001 gttagatgaa gtagaaaaag ctcatccaga tgttttgcat atgttcttgc aaatcttaga
     9061 tgatggtcga ttgacagatg cacaaggaag aacggtcagc tttaaagata cgatcatcat
     9121 tatgacaagt aatgccggaa ctggtaaagt agaagcaaac gttggttttg gtgcggcacg
     9181 cgaaggtgtt actcgttctg tattgaacca attaaacaac tactttacac cagaatttct
     9241 gaaccgtttt gacgggatca tcgaattcag tgcattatca aaagaaaact tgatgacaat
     9301 cgtcacacta atgctagacg atgtcaacca aatgctggca gcacagcaac ttcacatcga
     9361 agttcctacg aatgtcaaag aaaaattagt tgacttaggc tatgatccat ctatgggtgc
     9421 tcgtcctctt cgacgcacga tccaagaaca aatcgaagat ggtatcgctg aattttatct
     9481 tgatcaccct tctatccatg agttgaaagc aaaactggat aaagatggaa aaatcatcgt
     9541 tacttctaaa ccagaacgtt tggctaaaga atcagccgaa gaaacggctg aataaatcca
     9601 gttttatagc gtgagacggt atttctttga gaaatgccgt ctcattcttt tttcagaaaa
     9661 gtcaaaatac tgttcatttt ataaaaaaga aattataata agagaaacaa gcaattaaaa
     9721 atacataaaa gaggtgagcg ccatgaaatt ggtaaatgta acaaacagcc attcccgtct
     9781 tgtattaaac cagcttgaaa acactgatgc ccatctagtc aaggtctaca cagccggaaa
     9841 taccactatt gtctacactg aagcccctga acataatgaa attctgctga tcaatgacaa
     9901 acgtaaaatc cagccgaaag aaatcgaaga tgctaaaaat tatttcttag ataaaatcaa
     9961 agacgccgtt taccacgagg atgaaatcaa agtaatcgaa ttggcaggcc ttgttgaaat
    10021 ctcgattcca aaacatgaat taggcgctac attagccgtg taaacaaatt ttgaacataa
    10081 taattaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    10141 aaacggcgaa agcagaagca actccttcgg aaataagctg aaattcacga aaattgcttt
    10201 tcaaattttt gtgaattcca gcttatttct tggagttaaa cgcttctgtc tcagcctctt
    10261 tttctttcta gctttttgat ttttctcttt tcttttgtac ttttctcttt tcattttcag
    10321 ttctacgcgt gtctttattt ttaacttttt cctctttatg gatgattgat cgtacttttt
    10381 gtgcaatttg ctcgtttttg tcccagtctt ttacatctaa taatcgacat aatcgtaaca
    10441 tctcttcttt tttctgtttt tttctttcac gtctttgcca ttctgtttcc atgtgtcaca
    10501 ctctcctttc tttaatcata tgtacgcaaa ataattttta tatttttttg attagacatt
    10561 tctatgattt taatatagta aaaagagccg tcacggaaaa tccgtcacgg ctctaaacaa
    10621 aatatgagag gctgtttttg tttggctatt cttattactt gatgcagggc agcttgtgtc
    10681 aagacttctt tacatcaaac tttttaattt tacattggga tatttatccg caaaccagcg
    10741 ttctgcaaat tggttctcga ataagaacaa cggttggtcg aaacggtcac gagccaagat
    10801 attgcgactt gaactcatct tctcatccaa atcttctgga tcgatccagc gagcgatttt
    10861 gtgtcccatt ggagacatga ttacttcggc gttatactca tttaacatac gatattggaa
    10921 tacttcgaat tgtagctgac caacagctcc aatgatatat tcttccgtta gatatgtttt
    10981 ataaagctga atcgctcctt cttgaactaa ttgatagatt cctttgtgga aggatttttg
    11041 tttcatgaca ttttttgcag tcactttcat aaacagctct ggagtaaatg aagggagttc
    11101 ttcataagca acttttagtt ttccttcgaa caatgtatcc ccaatctgat aattgcctgt
    11161 atcatataca ccgatgatat cgcctgctac tgctttttcg acgttttctc tcgaatctgc
    11221 cataaattgt gtgacattgc ttaatttcag ttttttattt gtacgttcca accagacatc
    11281 cattcctcgt tcgaatgttc cagaacagat tcggacaaaa gcgattcggt cacgatgggc
    11341 tggattcata tttgcttgga tcttgaatac gaaacctgaa aattcttcct cataaggact
    11401 taccatctga tcatcttgtg ttttgtgtcc ataaggagct ggtgccaatt caacgaaagt
    11461 ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa ccaaaaaata ctggtgtctg
    11521 atcgccacga gcaatttttt cttgattaaa tgcatctcca gcctctttga ttagttctac
    11581 ttcttctaag acttgttcat ataaggaatt ttcagttaga ggatgatttt cgggaagcag
    11641 tccatcttca tccaattcta ctaatcgttc atcctgataa ttttccggac gataaaactc
    11701 tacacgatta tggtagatat cataaagacc ttctagccct ttacccatac cgattggcca
    11761 attcatcgga taagattcga tatctaatag ctcttctaat tcttctaaca attccaatgg
    11821 ttcacgacca tcacgatcta gtttgttgat aaaagtaaat atcgggatgc cacgtttttt
    11881 tacaacttgg aataatttct ttgtctgtgc ttcgatccct tttgcactat cgattaccat
    11941 gaccgcacta tccacggcca tcaaagtacg ataagtatct tcagaaaaat cttcatgtcc
    12001 tggtgtatcc aagatattta ctcgtttccc ttgataatca aactgcataa cagaactagt
    12061 aacagaaatc ccacgttgtt tctcgatttc catccagtct gatttggcaa aattacctgt
    12121 ttttttgcct tttacggtcc cggcttgacg gatagcccca ccaaacaata gtaattgttc
    12181 tgtaatcgtt gttttcccag catccggatg ggaaatgatc gcaaaagttc tgcgattgtc
    12241 cacttgttgc tttaattttg gattattcat aacctaactc catttcttcc attcaattat
    12301 ttatattttc tctaggcaca gattgtccga tctcttaaag aaagacaatt tcatataggg
    12361 ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa acggcgttct agagacagcc
    12421 tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc tgtttttgct tggctgctct
    12481 tattacttaa tgcaggatag ctttttcaca acctttgtca actactacaa ctgaatcagt
    12541 atagcatgga ttcagcgaat gaaaaagcca ccaaaccata agagtcaaat atttttttgt
    12601 cagctgacta aaagaaaaga cgtgttgctt gtttattcaa gcaacacgtc tttcataacc
    12661 aagtttccaa ttatcttctt ggctcgtcat cttcgaattc ttttctgaag tatcttcgtt
    12721 cttcttcagg ttccgcttct gtttcttccg gatcatgaaa aatgacacgg attttaagga
    12781 ttcgagaacc ttccatttct tcagaaacca gcgtaatatt atcgacatca aacgataact
    12841 tttcgccttc atccggaatc atgcctaatg ctgtaatcaa ataaccagcc atcgtatcta
    12901 catcactcat atgcagatca ctgtcaaaag cttcattgaa ctcatcaatc agcatacgtc
    12961 cttggatgat atattcgtgt tcatcgattt tttcataaag attctcaact tcatctgatt
    13021 catcatcgat ttctccgacg atttcttcta aaaggtcttc tagtgttgct aacccaacga
    13081 cgccgccata ttcatctaat aaaatagcca tctgattctg cgtctttttc atttcatata
    13141 acagatcatc aataaagacc gtttcaggaa cgaataaagg ctcctgcatg attttcttga
    13201 tatctaaatt atcaaagccg aatttatggg cagcttttag taaattcttt gtatgaagga
    13261 ttccgactac tttatctttg tcttcgttat aaacagggat tcttgaatag ttttcggata
    13321 aaacttcatt cacattttct tccacagtgt cgttgatatc aatcataaat gcatccgttc
    13381 gtgggaccat gacttctcgg gcaactttcg tatcaagaga aaaaacccct tggagcattt
    13441 ctaattcttc attattcaat acgccttcgt tttcaagcat ataacgcatt tcatctctgg
    13501 tcatttttga atcttcatca tcaaaagtca tcggcgtgat acgcgccaat aaacttgtgg
    13561 aagcagaaag cagccagacg aagggcttag ctactacacc aatcaagcgt atcagccctg
    13621 aagtgaactt tgcgacttct tctgatttat ttaaagcaat acgtttaggg tacaattctc
    13681 caaaaacaat cgatacatag gtcaaaatag ccagcacaat gatatttgca atattttttg
    13741 ccgctgtgcc gccacctaat accggagcta accgttccga taaagtatta gctagtgaag
    13801 caccagacaa aatgttgacc aaagtgattc ctacttggat tgtcgatagg aaattatttg
    13861 gattctgcaa tatttttaaa agcttttgag atttcacatc tccttcatca gctttttgct
    13921 cgattcgatt cttattgatc gaaacaacag caatttcgga agctgctaaa aaagcattga
    13981 tcaatgtcaa aacgatcaat aataaaatct gcgctaacag cgactgactc tcagggtcag
    14041 cattcataat cgcattctct ccttataaaa ttaattttta gtttacgata gaaaaaaacg
    14101 tggcactata gccacgttaa aatggtatca caaagcaatt ttctttgcaa taaaaacaag
    14161 tgtactaaaa ctacctacga ggtcgtagca attttcttct tgttttcatt ttgcttgtct
    14221 tctttgacga ttttgattat ataggaaatg atcgtcttgc agattgcgta aaaaggaaca
    14281 cctaagaaga taccaagcaa tccggcaata tttccagcta ctagaagaac gataatgatc
    14341 gttaatggat ggattttcag tgatttccca attacattag gataaatgat attcccatcc
    14401 aactgttgga cgattagcac aaccacacaa caaagtaatg cttttaccgg atcattgaag
    14461 actgttacta gaaatgcagg tgctagtcct agataaggtc ctaaatatgg aattaggttt
    14521 gtcaatcccg caatgacccc aaataagaaa gcataatcca caccaattac catatatcct
    14581 aaaaatgtga aggtaccaac gaataaacat tcgattgctt gtccgctaat gtaattagcc
    14641 aatgttttat tcagttgccc caataactcc acaatttggt ttcttctttt ttctgggaaa
    14701 aactgttgga tgtttggcac tagtttttct ccgtctttca acatatagaa caagatgaat
    14761 ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc caacgatcga acctaaacta
    14821 ttggatactc cactaaggaa ttgctgaatg attgtcccgt aagaaatgtc taattgctca
    14881 aaatatttat tgatatctac ttccttgaac aaaggcaatt cggctacctc ttttaaccag
    14941 ctttctacct gtttgatgaa atcaggcata ttagatgcca atgaggaaat ttgctgtact
    15001 agacttggga tgacactaag taaaatccaa ataattgctg caactaaaag gagcagcaca
    15061 attgcgacag cccatattct tttgattttt gctttcatca acagcttgac aataggattc
    15121 aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa ataacgtgga aaaaaatgtc
    15181 ccgatcggtt cgaaaataaa gtttatttct gatgaaatca tgatcaatgc agctatgatc
    15241 aataattcaa gcgaccaaaa cattagttta gactgtctaa gtttttcaaa caatagattt
    15301 cctcctttct ttcttactag tatatattat attcgttaaa aattctttta ttcttttttt
    15361 atttttgaaa acttgccata ctttttattt gttttatttt ccagtatact atacgaaagg
    15421 aaggtggcta gaccatgaaa gttgtacaaa caaaagatac aatgagcaat atttacttag
    15481 atgctgtacg gatcaggcat caagtttttg tagtcgaaca gggtgtgcct ttgtctcgag
    15541 aaatagacaa agatgaagcc cattgtatcc acttcgtcct gtactcagat aaaaaagaac
    15601 cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa aatgaaatta cagcggatgg
    15661 cgattctttc tgaatacaga caccaagggt taggaaaaat attgattgaa gaagcagaaa
    15721 actttgctaa gaatcaaggc tataatacga ttttattagg ggcacagtcc acagctgaaa
    15781 ctttttacga aaaactagga tatacagctt acggcgatcc ttttgaagat gctggcatgc
    15841 ctcatattta tatgaaaaag actttataaa aatttatttc gaaaaaagca aaaaacagca
    15901 agcttttgag aaaaatctct cactggctta ctgttctttt ttgtgtttac ttacgcgaca
    15961 attgcttgta acggttatac caaatatcaa tatagccttc cgagaacggg ccatctccat
    16021 tattgatcca gtcaaccaga atcttcacat tttcttttag gatgaagtca atatcgttcg
    16081 ggtaattcat cattttgctg tgcatctcat attcatcgac atccaatagt cgtttctcgc
    16141 cgtcaggaaa taccttgata tccaagtcat agtcgatata tttcaaggcc tcatcgtcca
    16201 gcaaaaaagg tgaagctaga ttacaataat aggaaacccc cttttctctg atcattgcta
    16261 ttacattgaa ccagtatttt ttatgaaaat aaacaatcgc tggttcgcgc gttacccatc
    16321 ggcgtccatc agattcagtg actaatgtgt gatcattcac gccgatcata gagtgttcgc
    16381 ttgtttttaa taccatggta tctcgccacg ttcgatgcaa tttgccgtcg tgcttgtaac
    16441 tttggatcgt cacaaactct ccttcttttg gaactcccat agcaaccctt ccttaagcag
    16501 cattttgtgc tttgtgtttg ggtgttcatt acaccgacat cataattatt atatcaataa
    16561 aaatgagctt tgcctagaaa ggattcctat tgtttcgatt tttctctaac tgatctacca
    16621 gtttcatttg gaccttagga aaaacgaccg aatcaaatgc atcaggcttc aaccacttcg
    16681 aagttttgtt ctcggtaaat tcttgttctg ctccctcggt tgcccgtccg taaaaaagga
    16741 gaacatgcca tttcagatga ctaaaaatat gagtaacttc tcctaaatgt cgtgtctgcc
    16801 aaacaaagag ttcctgcttt tcaaatggga gattttgctc ttcttctgct actaggtcat
    16861 caaacagatc gatttctggc tttgtttccc attctttttt caatcgctca tactcctctt
    16921 gtgtcacttc catcattgga aatgtccaca tattcgccag caatttttgg ctatctcttt
    16981 cttcaaaata atacgctcca gaatgatttt gaagagctgc actgatataa taaacgtctt
    17041 ttggctttgc tttttttgtt tttacaggaa aactcgtttg gattcctcgt ttgttagcta
    17101 aacaaaaggc ttggatcgga caagtctcac actttggaga agttggtgta cagatagctg
    17161 aacctagatc catcattgct tggttgaatt cacctggatg cttttcatca atgattttcc
    17221 gcatcgcttc atcaaagatt ttcctgctag aagcttttgc gatgtctgct tcaatgcaaa
    17281 acaatctgct gactactcgc atcacgttgc cgtcaactgc aggttctgga agtccaaatg
    17341 cgatacttgc aatcgcgcct gtcgtatatg gtcctattcc cttcaatgag ctaatttctt
    17401 caggcgtttg cggcatttcc ccatcaaatt ctgacatgat ctgtttagca gcagcttgga
    17461 tgttccgggc tcttgagtaa taaccaaggc cctcccatgc ttttaaaagc ttttcttctg
    17521 gagcatttgc tagctcttca attgtaggaa accattccat gaaacgataa aaataatcga
    17581 tcactgtgtc tactctggtt tgctgaagca tgatctcaga gatccagata cgataaggat
    17641 cacgattata gcgccaaggc aggtttcttt tttcttgttc ataccattga atgaactgat
    17701 cttgaaattc ttttgtttct ttatctgtcc atttttccca ttctttattc actttcctct
    17761 tccttttccg taatgaatcg ctggatcata tccaaatcaa atcctttttg atataagctt
    17821 tgctttactt ttagatttct ttttttaggt tcaaacctta aatttcttcg ccatagttta
    17881 tctccttgtt tgactaaata atcatattct atttcttgat ctgcttcttg cggaagatca
    17941 gctatcgctt gttggatcac ttcttgagaa aagcctttcg tcatcaatgt ttgttggatt
    18001 tttctcaata attctttttg gctttttccg tgattttttc caaacgcttt ttcggctgtc
    18061 tgatgtgcat tttgaacttg gtcttcaaat gtatattggc tgagcgcttg ttcaatgatt
    18121 tcaggtttca aacctttttg ctgcatctgc tgcgctaatt tctttggtcc tttgtcactt
    18181 aagcgcatat tcgtgcgcac ataactttcc ccatagacca aatcatcgac aaggtccaat
    18241 tcttttaagc gggctacgat tttatttcga tcttcttgaa ggatttcctt ttcttttaag
    18301 tatgttcgga tttctttttc cgatcgcaac tgataactta agtaattcat tgctaggtga
    18361 aaaccgaaat cagctccgct actcttcttg atctcttgga tagtttcttc gtctaattcc
    18421 cgtcctttta ataatcgata gcgtactaat acatcttcag atacttgtaa agcttctcct
    18481 tcagataaat ctacgcgata gaaagggcct ttgcctttac tgattctcgt aacggttaac
    18541 ataaacatcc tcctcgaaca tatattctct atcataccac agaaaatccc cgtaatcagg
    18601 tattatatgt tagaataaac cacagaaaaa agaaaaaggg agatacttat ggcagaaaca
    18661 attgtcaaaa ctggacaaac ggttcctttg aaaatcaagc gtttaggcat aaatggtgaa
    18721 ggaatcggtt atttcaaacg gttgatcata tttgttccat atgcactacc aaaagaagaa
    18781 gtactagtga aaatcacgaa agcaactcct cgctatgcag aaggagaatt aataaaagtc
    18841 aaaaaaacaa gcaaagatcg cgtagtggca ccatgtcctg tttactatga atgcggtggc
    18901 tgtcaattac aacacttagc ttatcaagct caattggatt ttaaaaagga tctgcttttg
    18961 caagcattgg aaaaattcaa accagcaggc tttcgatctt atcaactttt gccgacgatc
    19021 gggatggatg agccttggca ttatagaaac aaagcacaat ttcagttacg aaaaaataag
    19081 cagactcaaa aaattgaagc tggtttatac gaagcaaact ctcatcagtt agtccctata
    19141 acagactgct tggttcaaga acctcgaaca caaaaagtaa tgaatactgt tgttcaacta
    19201 ttgaataaat ttgatgcacc catctatgat gaacggacaa acagtggaat cttccgcaca
    19261 atcatggtta gaatcggtgt taaaactggg gagttacaag ttgtttttat tactcgcagt
    19321 aaaaaattcc cgcaaaaaaa cgcaatgatc cgtgagatca atgaacgact gcccgaagtt
    19381 gtttcaatca tgcaaaatgt ccaggcacaa aaaacgtctc tcgtgatggg ggatgaaacg
    19441 atccacttat ggggaaaaga agccattgaa gaacaaatca atgaagtgac tttcgatctt
    19501 tctcctcgtg ccttctttca actcaatcct cagcagaccg aattgcttta tggcgaagga
    19561 atcaaagcat tagatgtcca gcctaatgaa acgatcgtgg atgcttattg cggagttggg
    19621 acgatcggct taagtgtagc aaaacaagcc aaagaagtac ggggaatgga taccgttcct
    19681 caagcaatcg ctgatgcaaa aaaaaatgcg gaaagattag gatatcacaa cactcgatat
    19741 gaagtcggga cagcagagca agttctgcct aaatggttga atgaaggttt tcagcctgat
    19801 ggtatcatcg tagatccacc acgtacgggt ttggataacg cattgatcca atcattattg
    19861 aaataccctc caaaaaaact tgtttatatc tcctgtaatg tttctacact tgccagagat
    19921 ctggttaatt taagtaaaaa gttcaagatt gaatatttac agtccgtcga catgtttcct
    19981 cagactgcac gttgtgaagt tgtcgtgaag atgacgagac tcgctgacta aatgaaagag
    20041 gttgtgaaaa agtctgccct gtatcaagta ataagaacag ccaaacaaaa atagctcttc
    20101 atgttttatg aatactgtac ttgtgaccga agagttaaca ggaggacact atttatttaa
    20161 aaaaagggtt gtgacatagt ttttgtcaca gccctttttg attattattg gatattcacg
    20221 tctatcactt gataaaatgc atttgtcgta tcgctgatat tccatactgc taaaatcaca
    20281 ttatatcctt ttcgatcctg cggaagtgtg atcgtttggt tgactgtttt tggtggaaca
    20341 gttgctttgt cgtctatttt tgtgatcaat tcaaaatcac taaatttcaa aggttgatta
    20401 ggattccaag ttggtttggt aatgtagtaa tcccaagtac ttgtccgatg ttgtgctgtt
    20461 aaattccaag taatatttaa tgttcctgga ttaaccatgt ttttatgcca tctgttagat
    20521 gtctgttcat ctaaaggctc aaagccgctg actcctgcac tagcaatttt tccatcaatg
    20581 aatgtatttt tggtagcttc gatactttgc ggttcccatt gtgctcttcc gacattttga
    20641 tttaagtttc ctgctgctga tgttcctagg tatgcacggc ttcctggaga agtaatataa
    20701 ccatgcgcaa aacctggaat gctaaaaccg aacaacccta aactaactaa cccaataaac
    20761 aataacgtac taatcttttt catgtagaaa cctccttcat ttgataacca cattgtaaac
    20821 gctttattgt attaacacaa acattatgct tttataattt tatgttgaag taaaaaaggt
    20881 gaaaaaagta agatgcagat tataacagaa gtagacgaga aaagtacaaa tagaaaacat
    20941 gagaatcaaa tcatatgatc tttttcaaaa acatcattca tataaacaaa tgttcaactt
    21001 tttaataaca acaattatca atttttttct tacttcacaa actaaataca ggtcttgctg
    21061 tgagccttca tggtaaacta atattataga aaggagttag taaaatgaag atgataaaag
    21121 atattcgttt ttgggtttgt gttataggaa ttgtcatatt aggcttttta tcagggttgc
    21181 ttagcggaaa tcccggaaaa tattactatt ctttgcaact tccaccattt gcaccaccta
    21241 gctggatatt tgggccgatg tggaccttac tgtatatatt gatgggtatt tctctttatt
    21301 tgttacttaa tcacaacaac aaaaaacagc gaaacaatct tgtcggtctt tttgtgattc
    21361 agttcatttt taattttata tggtctgctc tattttttaa tttaagaaat attttcattg
    21421 cagcaatcga tattacactt ttagttattt tcttgtcagt gcttatgtat cagttgtggc
    21481 tgcatcatcg cctggcaatg tggctcatga ttccttatta tttatgggta ctattcgcaa
    21541 ctctattgaa ttattcaatt tattttttaa actaagcgct aaaaagagac gtcctgtaat
    21601 ttaacacata gaaaaagtgc cacctaagtt agatttt
//
LOCUS       DAYYAJ010000040        21600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000040 DAYYAJ010000000
VERSION     DAYYAJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.040"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACHATZ_001272"
     CDS             complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACHATZ_001272"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8403814.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACHATZ_001273"
     CDS             complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACHATZ_001273"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8403815.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACHATZ_001274"
     CDS             complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACHATZ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8403816.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACHATZ_001275"
     CDS             complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACHATZ_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8403817.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACHATZ_001276"
     CDS             complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACHATZ_001276"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8403818.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACHATZ_001277"
     CDS             complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACHATZ_001277"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8403819.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8344..8814)
                     /locus_tag="ACHATZ_001278"
     CDS             complement(8344..8814)
                     /locus_tag="ACHATZ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8403820.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8860..9285)
                     /locus_tag="ACHATZ_001279"
     CDS             complement(8860..9285)
                     /locus_tag="ACHATZ_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8403821.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9352..9621)
                     /locus_tag="ACHATZ_001280"
     CDS             complement(9352..9621)
                     /locus_tag="ACHATZ_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8403822.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9632..11227)
                     /locus_tag="ACHATZ_001281"
     CDS             complement(9632..11227)
                     /locus_tag="ACHATZ_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8403823.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACHATZ_001282"
     CDS             complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACHATZ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8403824.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACHATZ_001283"
     CDS             complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACHATZ_001283"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8403825.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15693..16667
                     /locus_tag="ACHATZ_001284"
     CDS             15693..16667
                     /locus_tag="ACHATZ_001284"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8403826.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16837..18237
                     /locus_tag="ACHATZ_001285"
     CDS             16837..18237
                     /locus_tag="ACHATZ_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8403827.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18315..19037)
                     /locus_tag="ACHATZ_001286"
     CDS             complement(18315..19037)
                     /locus_tag="ACHATZ_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8403828.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19116..19622)
                     /locus_tag="ACHATZ_001287"
     CDS             complement(19116..19622)
                     /locus_tag="ACHATZ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8403829.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19745..21544
                     /locus_tag="ACHATZ_001288"
     CDS             19745..21544
                     /locus_tag="ACHATZ_001288"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8403830.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6003 a         4717 c         3877 g         7003 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt
       61 ttgaataaat attttcttct gtcttttgga caagtaataa tataccaact aaatttagct
      121 tggtcaacga attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt
      181 ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt
      241 tgaactttcg ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta
      301 gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg
      361 catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca
      421 taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta
      481 gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt
      541 ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc
      601 gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa
      661 acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc
      721 cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt
      781 ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat
      841 ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg
      901 ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat
      961 tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat
     1021 caattccttc attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa
     1081 ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat
     1141 tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg
     1201 tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc
     1261 ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc
     1321 tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt
     1381 ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga
     1441 tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct
     1501 catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat
     1561 gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg
     1621 tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt
     1681 ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt
     1741 gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt
     1801 gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg
     1861 aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag
     1921 aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag
     1981 gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg
     2041 aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc
     2101 tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg
     2161 tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt gaatcatcca
     2221 tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat
     2281 ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa tcgtcaaatg
     2341 ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt
     2401 acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac
     2461 agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac
     2521 tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc
     2581 tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat ctattatatc
     2641 ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc
     2701 aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt
     2761 tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc
     2821 aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac
     2881 gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc
     2941 taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca
     3001 gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc
     3061 acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac
     3121 aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc
     3181 tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc
     3241 gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc
     3301 cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg
     3361 ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt
     3421 ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt
     3481 tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg
     3541 atttttgatg taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc
     3601 tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag
     3661 tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg
     3721 acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc
     3781 gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt
     3841 tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct
     3901 tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata
     3961 aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg
     4021 acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc
     4081 tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg
     4141 tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa
     4201 agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc
     4261 gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc
     4321 agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc
     4381 ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc
     4441 ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg
     4501 acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg
     4561 ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg
     4621 tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc
     4681 ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt
     4741 gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata
     4801 agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat
     4861 cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc
     4921 agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt
     4981 ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac
     5041 tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa
     5101 acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac
     5161 gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg
     5221 ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat
     5281 ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac
     5341 acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa
     5401 cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg
     5461 tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc
     5521 tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc
     5581 agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt
     5641 catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa
     5701 aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct
     5761 tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct
     5821 caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga
     5881 acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt
     5941 gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag
     6001 ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat
     6061 gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg catttttcat
     6121 catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt
     6181 tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt
     6241 gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca
     6301 taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct
     6361 tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt
     6421 gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata
     6481 tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt
     6541 acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc
     6601 aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca
     6661 cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg
     6721 caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc
     6781 aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat
     6841 cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc
     6901 ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga
     6961 taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt
     7021 ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag
     7081 accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt
     7141 ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct
     7201 ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta
     7261 tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa
     7321 tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa
     7381 aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa
     7441 atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt
     7501 gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct
     7561 gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat
     7621 ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat
     7681 ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt
     7741 gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca
     7801 ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt
     7861 ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt
     7921 tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc
     7981 gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac
     8041 ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat
     8101 caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat
     8161 tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt
     8221 tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac
     8281 aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc
     8341 tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga
     8401 agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt
     8461 ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc
     8521 acggggatct ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg
     8581 ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt
     8641 aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga
     8701 cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa
     8761 atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa
     8821 aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact
     8881 agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac
     8941 tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc
     9001 atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat
     9061 ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa
     9121 aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct
     9181 gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc
     9241 atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa
     9301 tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc
     9361 tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt
     9421 ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt
     9481 caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat
     9541 tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt
     9601 taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca
     9661 ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa
     9721 tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag
     9781 cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca
     9841 gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc
     9901 aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc
     9961 ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag
    10021 tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct
    10081 tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa
    10141 ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga
    10201 gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga
    10261 ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag
    10321 gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac
    10381 ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca
    10441 aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa
    10501 tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga
    10561 acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa
    10621 ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt
    10681 ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac
    10741 gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga
    10801 aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga
    10861 tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga
    10921 caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt
    10981 gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac
    11041 cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa
    11101 aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg
    11161 ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat
    11221 gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac
    11281 aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg
    11341 tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc
    11401 ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat
    11461 atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg
    11521 cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc
    11581 atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc
    11641 gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc
    11701 gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc
    11761 gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc
    11821 acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga
    11881 tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat
    11941 cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc
    12001 tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt
    12061 tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg
    12121 accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc
    12181 ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt
    12241 ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc
    12301 ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc
    12361 aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct
    12421 cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg
    12481 ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct
    12541 aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt
    12601 acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc
    12661 gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa
    12721 gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc
    12781 tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg
    12841 tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc
    12901 agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata
    12961 tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat
    13021 ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg
    13081 aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat
    13141 aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac
    13201 ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc
    13261 tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct
    13321 cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc
    13381 ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat
    13441 caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc
    13501 tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca
    13561 acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata
    13621 ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa
    13681 aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg
    13741 ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc
    13801 gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga
    13861 agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc
    13921 ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa
    13981 aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat
    14041 ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac
    14101 ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga
    14161 atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat
    14221 atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac
    14281 atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg
    14341 ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc
    14401 gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct
    14461 ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg
    14521 aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc
    14581 ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc
    14641 aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc
    14701 ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc
    14761 cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt
    14821 ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat
    14881 tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc
    14941 cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga
    15001 taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc actgccagat
    15061 ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg
    15121 ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt
    15181 ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt
    15241 gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt
    15301 atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc
    15361 taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc
    15421 ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa
    15481 tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat
    15541 ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg
    15601 ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg
    15661 aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc
    15721 atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag
    15781 ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag
    15841 gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg
    15901 ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa
    15961 aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg
    16021 tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag
    16081 atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt
    16141 caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg
    16201 atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt
    16261 ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa
    16321 tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta
    16381 tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg
    16441 ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg
    16501 gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag
    16561 taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat
    16621 taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga
    16681 cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa
    16741 ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc
    16801 gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa
    16861 aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt
    16921 tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga
    16981 ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct
    17041 gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag
    17101 ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga
    17161 tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt
    17221 caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg
    17281 aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt
    17341 atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa
    17401 agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa
    17461 gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat
    17521 gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg
    17581 agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat
    17641 gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca
    17701 aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt
    17761 gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt
    17821 gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat
    17881 ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc
    17941 gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa
    18001 gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac
    18061 tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa
    18121 tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt
    18181 gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa
    18241 aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc
    18301 taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct
    18361 tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc
    18421 gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct
    18481 cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc
    18541 agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac
    18601 ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt
    18661 tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta
    18721 cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac
    18781 gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc
    18841 acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga
    18901 ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc
    18961 tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat
    19021 ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta
    19081 gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca
    19141 aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg
    19201 aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct
    19261 gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca
    19321 cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa
    19381 aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa
    19441 ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat
    19501 cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg
    19561 actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc
    19621 ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata
    19681 gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt
    19741 tttcatgagt tattcattaa attggaatct agattctatt ttttccggag gcagtcattc
    19801 tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg
    19861 cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt
    19921 gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc
    19981 tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc
    20041 tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga
    20101 ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat
    20161 tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc
    20221 tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat
    20281 tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat
    20341 gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg
    20401 gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc
    20461 tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt
    20521 gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt
    20581 taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca
    20641 agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc
    20701 agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc
    20761 tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta
    20821 ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt
    20881 taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg
    20941 ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt
    21001 cgcagaattg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat
    21061 caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg
    21121 attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa
    21181 gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta
    21241 ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa
    21301 cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa
    21361 gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac
    21421 ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc
    21481 aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat
    21541 ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
//
LOCUS       DAYYAJ010000041        20746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000041 DAYYAJ010000000
VERSION     DAYYAJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.041"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..416
                     /locus_tag="ACHATZ_001289"
     CDS             60..416
                     /locus_tag="ACHATZ_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403831.1"
                     /translation="MEGKRMEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKK
                     HLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVE
                     KAAEMNSLANEVSRLI"
     gene            complement(409..600)
                     /locus_tag="ACHATZ_001290"
     CDS             complement(409..600)
                     /locus_tag="ACHATZ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFM8403832.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            950..1162
                     /locus_tag="ACHATZ_001291"
     CDS             950..1162
                     /locus_tag="ACHATZ_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403833.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            1169..1393
                     /locus_tag="ACHATZ_001292"
     CDS             1169..1393
                     /locus_tag="ACHATZ_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403834.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            1420..1953
                     /locus_tag="ACHATZ_001293"
     CDS             1420..1953
                     /locus_tag="ACHATZ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFM8403835.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            1940..3184
                     /locus_tag="ACHATZ_001294"
     CDS             1940..3184
                     /locus_tag="ACHATZ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFM8403836.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            3197..4696
                     /locus_tag="ACHATZ_001295"
     CDS             3197..4696
                     /locus_tag="ACHATZ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8403837.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            4701..5627
                     /locus_tag="ACHATZ_001296"
     CDS             4701..5627
                     /locus_tag="ACHATZ_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8403838.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5754..6356
                     /locus_tag="ACHATZ_001297"
     CDS             5754..6356
                     /locus_tag="ACHATZ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8403839.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            6369..7292
                     /locus_tag="ACHATZ_001298"
     CDS             6369..7292
                     /locus_tag="ACHATZ_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8403840.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            7310..7573
                     /locus_tag="ACHATZ_001299"
     CDS             7310..7573
                     /locus_tag="ACHATZ_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403841.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            7585..7914
                     /locus_tag="ACHATZ_001300"
     CDS             7585..7914
                     /locus_tag="ACHATZ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFM8403842.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            7911..8237
                     /locus_tag="ACHATZ_001301"
     CDS             7911..8237
                     /locus_tag="ACHATZ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403843.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            8221..8559
                     /locus_tag="ACHATZ_001302"
     CDS             8221..8559
                     /locus_tag="ACHATZ_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8403844.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            8559..8951
                     /locus_tag="ACHATZ_001303"
     CDS             8559..8951
                     /locus_tag="ACHATZ_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFM8403845.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            8962..9474
                     /locus_tag="ACHATZ_001304"
     CDS             8962..9474
                     /locus_tag="ACHATZ_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFM8403846.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            9520..9888
                     /locus_tag="ACHATZ_001305"
     CDS             9520..9888
                     /locus_tag="ACHATZ_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFM8403847.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            9942..10229
                     /locus_tag="ACHATZ_001306"
     CDS             9942..10229
                     /locus_tag="ACHATZ_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403848.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            10246..13365
                     /locus_tag="ACHATZ_001307"
     CDS             10246..13365
                     /locus_tag="ACHATZ_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8403849.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            13377..14165
                     /locus_tag="ACHATZ_001308"
     CDS             13377..14165
                     /locus_tag="ACHATZ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFM8403850.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            14162..16993
                     /locus_tag="ACHATZ_001309"
     CDS             14162..16993
                     /locus_tag="ACHATZ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8403851.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            16968..18725
                     /locus_tag="ACHATZ_001310"
     CDS             16968..18725
                     /locus_tag="ACHATZ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8403852.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            18742..19524
                     /locus_tag="ACHATZ_001311"
     CDS             18742..19524
                     /locus_tag="ACHATZ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403853.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            19524..>20746
                     /locus_tag="ACHATZ_001312"
     CDS             19524..>20746
                     /locus_tag="ACHATZ_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8403854.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIK"
BASE COUNT         7259 a         3341 c         4522 g         5624 t
ORIGIN      
        1 caactccttc taaattgttc ttattatcta tcatccgttg ctgtttatta atttatgtat
       61 tggagggaaa acgaatggaa gtcgagcaaa taaagtatca aggtatggat cgggaacggt
      121 tgattgatat tattgagcaa caacgtttag aattgatcca aaaagaagct gtatgtaaag
      181 agtacaaaaa acatttagaa caagtaattg agtatcattc agtagaaaag tacagatcag
      241 tggtgcaaaa aaatagagca acgagtcctg atcctcgtca agatgtatcg attagtatca
      301 agacgccagt atttaattct gttaaatcag aaaagctaga aagtatctca actgctttgg
      361 tagaaaaagc agctgaaatg aactccctag caaatgaagt tagtcgtctt atttagtcac
      421 atcgataact tgagagtctt tggtgattcc gttttttata gaattaatgg tttgttcagc
      481 ggaatattta gtcaagtatg tttcgctagt tgctactact tcattgttat cggacttaat
      541 aacgaagtaa tattggccat tagttgcttc tcttataaca aagtacaaat ttttcaccac
      601 cttttaattt atttcagcgg accactcgct gataaataaa attatacact tagtatttat
      661 tttcacaata ttaatttgtc actgtggcgg aaagggtaga cgctaagcat gtgtgttagg
      721 tcaatgcttc ggcaaccatg caaggttcga ttccttgcca gtgactttgg caactgaggg
      781 ttggaaatgg gcgctcaaag tacacgagca aggcgaggtc gatagtaatc gatggaatcg
      841 gtgtaggttg cttgatagaa ctataactgc atctcgttta tgaggtgtag tttttacata
      901 ttagatcact catcgagtgg tctttttatt ttggaagggg agtaaaaaaa tgaacgaaaa
      961 ccaattaaga gagttgttta aaacgaatga agcaaacaaa acaatggagg cgacattcta
     1021 cgaaactcaa aaaagcttag cgttaatcgc aaaacaagct aagtatttct acgatcagct
     1081 tattctgcaa ggatttaatg acgcacaggc tatggaattt atgatgcgaa ccttttctgc
     1141 cagtaaccaa cagaaagagt gatacgtaat gaggaactac tggtatgtat cattatcaaa
     1201 taaatatccg caaccaaacg cagatgatcc aattagagtt gtccagtcag tccagattaa
     1261 aaagaagtac tccatcattg aaatgaccag agaagctacg ccaaaagaga ttgataagta
     1321 caatcttcgt tactgcggcc atggatattt tagtgagcag aacatacaga caaatattga
     1381 gagatattgt taataaaatt gaaaggtggt ggcttgaatg tggtaaattt gacaccaaaa
     1441 caaaaagctt ttgcggatga atatataaaa aatggtggta atgctactca agccgccatt
     1501 aaagcaggtt atagcaaacg atcagcaaga gtaataggta aggaaaacct aactaaacct
     1561 aacataatac agtatattaa tgaacggcta aatcctatcg aaaagaagcg caaattaagt
     1621 gccgaggacg ctttaaatga attgatagat atttggcaag gagaagtaca aataagcgtg
     1681 agcaagcaaa tagaccgctt ggagaaaaac aaggttatta aacatatgca atatgaatat
     1741 acaccagatt tagaaagcaa attgaaagcc ttggatttgt atttgaagta taaatcgctg
     1801 ttatcacaaa cgcaattaga aaaagctcaa acagaaataa aattaatgca agcaaaatta
     1861 gaacaattac agataaactc agagcgttct accgaagaaa aacttgatga gttgttagaa
     1921 aagattagtg gtgaattaga tggcactagt tgatatttat aacccaaagc aaatcgacgt
     1981 gttaaataaa accattaaga atgattggtt tattacttta ttacatggag caaaacgttc
     2041 tggaaaaaca aaaataaaca atgatttgtt cttgtttgaa ttgcgacgtg tgcgaaagat
     2101 tgccgatgaa gaagacatta aggaaccaat gtatatccta gcaggagttt caagtgcaac
     2161 aatccaaaag aacatcttac aggaactata caacatgtac agcatagaac ctaaattcga
     2221 taaacatgga aactttaagc tatttggcgt taaggtcgta caagcttata caggaaacat
     2281 tggcggtgtt ggcgcaattc gtggtatgac agcatatggc gcttatatca atgaggcatc
     2341 gctagctaga caagaagtat ttgctgaaat cgtttcacgt tgttcaggaa caggagcgag
     2401 aatactagct gatacaaacc ctgacaaccc ggaacactgg ctaaagaaag agtatatcga
     2461 caattcaagc aaaaatattc aatcgttcca ctttgaatta gatgataata catttttatc
     2521 tgaacgatat cgtaacaata ttaaagaatc aacaccaagc ggcatgtttt atgatcgtga
     2581 tatcaaaggt ttatgggttt ctgcagaagg tgtcgtttat cgtgattttg atgctagcaa
     2641 gcactacatc cagtcaaaag acttaccacc tttgagcaac ttttattgtg gtgttgactg
     2701 gggatatgaa cactggggtt caatcgtagt tataggtgaa acggatgacg gaacagctta
     2761 tttaatcgaa gaacatgcta ctcaatttga agagattgat tattgggtag atgtggcaaa
     2821 aggcattcaa gagcgttacg gcttacgagt acctttttat tgtgactctg cgagaccgga
     2881 gcatgtggct aggtttgtaa gagaaaagat taatgctaaa aatgctcata aagcacggtt
     2941 atctggagtt gaggaagtcg ccaagagatt taaacaagat aaattgttta tctgtcaaga
     3001 tagagtgatg aaatttcggg atgaaattta tcaatacatt tgggacaaga aaaaaggcga
     3061 gccaataaaa gaatatgatg atgtgctaga ctccgttcga tatgcgatat atactcatga
     3121 gcttcttaag aaaccaaaag ttaatgtcaa cgaaaagatt aaacgtatta aacgcatgtt
     3181 ttaaggagtg tgaaaaatgg ataaggtaaa cgagtttgaa tacggagctg atatacatta
     3241 ttctaatgac gtgaacacaa attatgtaaa gtttagcgta gactcaaatc ttcactatag
     3301 atttagctca gcagaagatt tacttaacga tttagatact ttagcagcaa tgataaaaca
     3361 tcatcatgaa tatcaggtaa aaaggctaag tgtattagat gattattata aagctagaaa
     3421 tacaaatatc atggataacc gtaggcgcag agaaaaagaa aaagcggatc accgatcagc
     3481 acataacttt ggaaaagttc tttgtacgtt tgatgttggg tacaacacag gcaatcctat
     3541 aaaagtgcaa atcgaggaca caaatcaaca aaaagaaatc gaagagttta atactaataa
     3601 tgacatagat gggttaaatg ctgaactctg gcttgatatg gataagtatg ggagagccta
     3661 tgagattatc tatcgagatt cagatgatac agattatgtt gatttggcta atgtatttga
     3721 aacgtttgtt gtatatgata ctacagtaaa acgagagcct attttggctg tacggtatcc
     3781 taagacaaga ttcaacaagg atgctgataa acagtacatt caaccaatcg tatacacaaa
     3841 agaaaaaagt atcacttatg atgagacgac actaacagca attgagttaa agaatcccca
     3901 ggatgaaccg catgaatata aagaggtacc tattacagag tattctccta atcgttttcg
     3961 gatgggcttg tatgaagatg tactatcttt gattgatcta tacgatgcag ggcagtctga
     4021 taccgccaat tatatgactg atctaaacga tgctcttcta gttattagtg gtgatattga
     4081 agcagcagga ctatccacag aggacgccat caagcagaaa gaagcgaata tgcttttgct
     4141 tgagtctgga actgatgtga acggtaataa aacaagtgtg actgcaggat atatttacaa
     4201 acaatatgat gtgaacggtg tagaagcata caaatacaga gtacgcaagg atattcacga
     4261 aatatccatg gttcctgatc ttactgatga caatttttcc ggagtgcaat cgggagaagc
     4321 aatgaaatat aaattatttg gatttgaaca aatgacggca acaaagcaaa ggctattcaa
     4381 aaaaggcctt atgcggcgtt atcgtctttt atttagccta aaatcaagta tttctgaaat
     4441 ggataactcc gatttgaaag gcttacgtgt aatatttacg cctaatctac ctaaagccat
     4501 tctggaagag ttgaaatctt tggttgatgc tggagctgaa ctcagtcaag agacgatctt
     4561 aggactcgct tcttttgttc cagatgtaca ggcagagttg aaacgagtaa ataaagaaac
     4621 gcaaaagcag attggcattt ttgattcaga tggtgaagaa gtaattaaca acaaaaaaga
     4681 tgaaacaggg gggtgattaa atgaactccc aagaatattg gatcaaacgg gaaaaggaat
     4741 ggcaaaagca acaaattaaa gatgataaaa agcgcatggc agaaattaaa agtcgcatgc
     4801 aatacgcaca agatgcgata caaaaagaaa tagacgcgca gtgggacagt ttctccaatg
     4861 gccagaaaat cactcgtagc gaagcgatga agcgtgctag tgaaatggat gtcaaagcat
     4921 tcgctcgcaa agcaaagaag tatgtcaaag agaaagattt ttctcctaca gcaaaccaag
     4981 aattaaagct atacaatctt acgatgcgtg taaatagatt agagctctta aaagctaata
     5041 tcgggcttga attgatttca ctgtttaatg aattggataa gtacttttcg aatgaattaa
     5101 caaaagctgg tttagctgaa ttgaagagac aagccggtat tttagaaatg actattgctt
     5161 caagtggata tgcaaagctg atagaactag taataaacag ctcctttttg agtgatgacg
     5221 tgtcttttag tgatcgctta tggatgtatc aatctgaatt gaaatcagaa ttagataggt
     5281 tgttagtcag aagtataacg atggggaaaa atcccaagca acttgcatct aaattggcag
     5341 aatatttaac agctgaagga cgagaaaaca ctaagttcaa cactcaacgt ttgatggtga
     5401 ctgaaacgac tagagttcag gtagggattc aagaacgaag ttatagagat gcaggcatta
     5461 cccagtacat ctatatagca gaaccaacgg cgtgcaaact atgtataccg ttaaataatc
     5521 aagtttttga tgttgccgat atgcagccag gaagtaacgc tcctaatatg catccatttt
     5581 gtcgatgcag tacagcacct tatatagaac gaatatcaag ccgttaacac aaattaacgg
     5641 ctttttattg tgccttctta cagcttacag gcgttaaaga gaaagctatt ttcggctgac
     5701 cggcgtaact ggtcaaattt atcgggtagc ggcgtaaccg tggaggatta atcatgaaaa
     5761 aacgtttatt tatgccaatg aacttacaat ttttttctga accaggagat ggtggatctg
     5821 gtgatgaggg acaacaagga aacctaccag ctggctcaca agagacaccg cccgaagcaa
     5881 aagaagaaaa caatactggc aaaacatttt ctcgtgatga agtagcgaaa atgatcgctg
     5941 ctgagacgaa taaagcaaaa gcagcgtggg aaaaagaact agcagcaaaa aaagaagaag
     6001 ctaaaaagct ggcaaaaatg aatgcggaag aaaaactaca gcatgagttg gagcaaaaag
     6061 aagctgaaat cgctgaatta aagcgtggac aggcactatc tgaaatgacg aaagaagctt
     6121 ctaaaatgct gacagatgca aatttaccac acgatgatga tttacttggt ctgattgttt
     6181 ctgatgatgc agatgccaca aaacaagctg tagcagtcat cactaacttt gcttctttga
     6241 ttaagagaga aaacgcaaga caaacaccac caaatgaagg tggacaattt acagcatcga
     6301 aaaatactaa agaaacagtg gctaaactag ctgctaaaaa tcgaattatc aaataggagg
     6361 aaaacttaat gaaaaagaaa caacttttac caatgaactt gcaaatgttt gctcaaacat
     6421 gggatccaga taatgtcttg gtatatgaaa cgaaagaggg aaaaattcct gataaatata
     6481 atacgctcat tttgagtgaa gttatggaaa attctaagat catgcagtta gcaaaatacg
     6541 aagaaatgac tgacaaagaa aagaaatttg aatactttgc agaaggacca ggcgcatact
     6601 gggtgggtga aggtgaaaaa attaaaacgt ctaaacctaa atggatgcaa gctacgatga
     6661 ctgcaaaaaa actcggtgtc attcttccgg tttctcgtga atatttaaat tataaattat
     6721 cagatttctt tgaggagatg cagccaaaaa ttgctgaagc tttctataaa aaatttgatg
     6781 cagctgcctt attaaataaa gaaaacccat ttcctcagtc actagacgga tcagttatta
     6841 gtgcggggaa tgtggttgaa ggcggattga cttatgataa tatcctagcc ttagaagaca
     6901 agttagcaga aaatgaattc gaacctaatg cgtttatttc aaaccgaaaa aatcgtacag
     6961 aattacgttc tgcagctcaa acagtcgggt caaatgttga gtttatttat gatcgctcta
     7021 ctaatacaat tgacggatta ccagtagtag accttaagtc tttagataaa ggaacacttt
     7081 acgctggaga ctttaattac atgttttatg ggatcccata taatatttca tttaagattt
     7141 ctgaagaagc ccaattgtct actttaacta atgaagatgg aaccccagtt aacttgtttg
     7201 agcaagaact gattgctttg cgtgcaacaa tggatgttgg atttatgatt gtaaaagatg
     7261 aagcatttgg gaagatttcc ccaaaagcgt aacgcctgct accggtattg tgccaaatca
     7321 aaagacatgg accggtaaag taggcgatac taaaacattt actatttcag ctgtgcctgc
     7381 agatgctagc gatgcagctg ctgttgttgc agctactaca gcaacttcaa gtgatggagc
     7441 tatcgcaaca gtgaccaaaa atgaaaatgg tggttttgat ggaacgattg cagcagaagg
     7501 gtcagcaaca ttcacattta cttctggaga attcactact tcaatcaatg tgacaggtca
     7561 acctgctagt taggaagtaa aaatatgatg attgcagatg atattaaaaa acttcttaaa
     7621 ggaacaatag atgaaaagct tgaagttatt gagcgaagaa cgaatgagcg tatgaaaacc
     7681 ttgttaaata cgcaagaagt tcctaaagaa tttgaaacag ttgtatatga agtatcgttg
     7741 aaaagattca atagaattgg tcaagaaggt atgcagtcat attctcaaga aggtttatct
     7801 atggcttttc ctgactcgga tttttcagag tatcaaaatg agattgatga atttaagcgt
     7861 aaagatcagg aagagttgta caagccaaag cgagggaggt ttaaatttgt atgagattta
     7921 cagatgaaat tatatttgtt aaacgttcat ctgactctaa atatgatcca gatctcggtg
     7981 agtgggttga aggcaaacca gaaagaacaa gaacagaggc aaacgtgaca gatattggca
     8041 ctgatagaag tgtgactatt tttggtagtg tggaagaagg ggcgaaggtc attaggacgc
     8101 agcctctttt ttctatccct acatttgact atatcgagat tgaaggaaag acttggcaac
     8161 aaaaaacagc tagaaatcca gcatatagaa atagtttaat tgtgcaagag gtggttcttg
     8221 atgaaggcac aacttgaata taaaggaatc gatcagctga tgcgacatct gaaaaaagca
     8281 gcaacgctta atgacgttca aaaagtcgtg aaaagtaata ctgctgaaat gactgaacga
     8341 atgcaaaaag gtgcgccagt ggatacaggt tacttacgaa gatcaataaa catgaatctt
     8401 ttagaagctg gtttaactgg tattgtagga ccgacagcag attatgcacc ttatgtagaa
     8461 tatggcactc gatttatgtc ggcacagcct tatgttagac cagcgtttaa ttaccaaaaa
     8521 gtcaaattta tggctgaaat gaaagccttg gtgaaatgat gattaagaca agagatcaat
     8581 caatttttga tgaacttttt aaaatatccc aaaacaaact tggatataaa acatacgatt
     8641 acaaaacttt agaggatgtt ggttatcctt ttgtggaatt tgagaacact cagacaatcc
     8701 atgaagtaaa taaaactgac attaaagggt ctgtgattgt ggttttatcc gtttggggat
     8761 tacagaagaa acgaaagcag ttgtcagata tggcatctgc tctttttaat gaagctagat
     8821 tgatagaagc cacagaaggc tattattggg ctttaaatta tcaagcaagt ggaattcaag
     8881 tgatggacga cacaacaacg aatacaccgc taaaacgggc ggttgtcaca cttgaattta
     8941 gaattagata ggaggaagaa catggaagca ttaaaaggta ttgatgtcat tttgctttat
     9001 cgcttattga aaaaagaaac tcaggaagcc gcttggaaaa tggcatttca aacagaacac
     9061 gaaaatggtt tatcaagaga ttcagactct acagtgacaa aagatggaaa cgttcaaagt
     9121 ttaagcccgg ttgaatatga tttttcggct acttcaatag ttgctaaagg agattctcat
     9181 gtagatgaaa tgaaacaagc tttattaaat ggggatatca ttgaaatttg ggaaattaat
     9241 aaagcagaac agggaacaga tgataatgca aataagtaca aagctactta ttaccaagca
     9301 tatgtgtctg aatttactcc atcggctgct gcagaggata acgttgaatt aagtttatca
     9361 tttgcagtaa atggtgttgg tcaagatggt tatgcaacct tgacagaaga tcaagccgat
     9421 gttgttcaat atgcattcaa agataccgtg aaagcaactt cgacaggagc ataagagggc
     9481 ttagatgctc tcttttttat tttaggagga tgaaaaacat tgaaattaaa aattaaaggt
     9541 aaagaatatt cgtttaaatt tggcactaaa tttgtacgtg aattagataa agtgatgcct
     9601 ttcgtcgatg gaaatatgga atttggaatg ggactctccg caaaagtctt accagagcta
     9661 cgttcttata acgttaacac gttatcacga gttctggaaa tagcaaatag aactgaagat
     9721 gaatctatta cattagatga attggatgat tacatcgatg aagttaaaga cattgaaaaa
     9781 ttgtttgatg aagtcctaaa agaattggcg gagtcgaacg cgggaaagtt agcggtccga
     9841 aacctgaatc agaaattgaa agaagcggaa aaacaacaag tggaatagat tctgcactgg
     9901 catacgaaca aattcttatc aattcttttc gatatttggg aatgaccaat atctcagata
     9961 tcgaaagaat gacgttatat gaatacaaca ttcgtatgac tgcagcccag ttatcttggc
    10021 ttgacaaaga aaagttgatt cacgaattag cgtgggcaaa tcagcaagtc caagcggaga
    10081 aaaaagtagg caaaaagaca gttcctgtat atcgatcctt tgaagaattc ttcaattatc
    10141 aaaaaatcga agattcaatc atgggagttt ccgaactttc aaaacaagat aaaaaattcc
    10201 aaagcttact aactaaagct aactcttgag gaaaggagga gaatcatgga acaattttct
    10261 gttgaagcct tattaaaagc cacagatagt ggatttgtaa agacttttaa agatgcacaa
    10321 gatgctgtta aaacttttga aaagaattca aatagtatga caaccgctgt tggtaaagtg
    10381 atgcaaggta ctggtgccgc aatgacaaag tatattacca ctcctcttat aggagtaggc
    10441 gtagcagctg ctaaagttgg tggcgacttc gaagcacaaa tgagtcgtgt aaaagctata
    10501 tcaggagcaa ccggcgacac attcgaacag atgaaacagc aagcgattga tctaggagca
    10561 aaaactgctt ttagcgcaaa agaatcagct gctggaatgg aaaacttagc ttctgctgga
    10621 tttagcgcac aagaaatcat gaaagcaatg ccgggtcttt tagacttagc agctgtatct
    10681 ggaggggatg tggctctagc ttctgaaaat actgctactg ctttgagagg atttggttta
    10741 gaagcaagtg aagcaggaca tgtcgctgat gtatttgctc gtgctgctgc ggataccaat
    10801 gctgaagttg gagacatggg agaagcattg aagtatgttg ctcctgtagc taattcaatg
    10861 gggatttctt tggaagaaac tgcagcagct attggtatta tgagtgacgc aggcattaag
    10921 ggttctcaag caggtacaac gttgcgagga gcattgtcta ggttagcaag gccaacaaag
    10981 gctatgcaag atacaatgga taatttaggt gtttcgtttt atgatgctga cggtaaaatg
    11041 aaacctttaa aaactcaagt agaattactt aaaaaagctt ttgaaggcct gacgcctgaa
    11101 caacaacaaa atgctttagt aacactatat gggcaagaat cattatcagg gatgatggct
    11161 ttgattgata aaggacctga ttcattgggc aaattaacaa aatctctgaa agattctgat
    11221 ggtgcagctg acgatatggc tcggaccatg caagataata tgaattcttc catcgagcaa
    11281 atgtttggag cttttgagtc agcagctatt gtaattcaaa agattctagc accatccatc
    11341 aaaaaagtag cagatgccat atccggctta gtagaaaaat ttgtaagtgc tccagaatca
    11401 actcaaaaat tagtagtggc cataggagca attgtcgctg ctataggacc gttaattttt
    11461 atgattggtt cagtaattat atggatcaat agggtgaaag tagcttttaa agctttaagt
    11521 gaaagttcaa aattgtttag tggattaagt aaagcaatgg gtcttcttac aaatccggtt
    11581 tttctggtta tagctgcggt agcactactc gttgtaggtt tcatctatct ttggaatacg
    11641 agtgaagatt ttagaaactt ttggattggc ttatgggagg gaatcaagtc tgctgtaagc
    11701 tcggcagtag aatggattca gaatgcatgg aaatctacag gagaatggtt taacaattta
    11761 tggaagtcca ttaaagaagg cgcagacaat gtttggacta cagttcaaga agcccctggg
    11821 aaagcggcag attggattaa aaacaaatgg actggaacaa aagagttctt ttcaaattta
    11881 tggtcaagta ttgcaaactc tgcttcagag atttggaata gtttaaaaga aggtgtcata
    11941 tcagttattg atgatttagt ttcaagtgct ggtgaaaaat gggaagggtt taaaaatact
    12001 atatctactg catggaaaac aattacaagt aaaatcaaat ctggttttga ttttatacta
    12061 aaatatattg gtccatttgt aagtagcttt tcagatgtgt tctctaatat agtgaaagca
    12121 ataacaagta tatttgctga ggttaaaaac ataatagtaa atgcttggga aatcattaag
    12181 tctttaatag ctgcgccact actgtttatt atagatttaa ttactggtga cttcgaacaa
    12241 atgaaagagg atttagatct aatctggaac acacttgtcc aatcagtggt aaatatttgg
    12301 acatctgtaa aaaatatatt tacggaatat atcggtgcaa tagtaaatag tgccgttagt
    12361 ttatggactg gattcataca aagtatttct aatatttgga atgaagtagt ttatcaagcg
    12421 actatgattt ggattgattt gaaactattt tttactaatt tatggattga tattaaatac
    12481 agtgcaattc aaatgtggat aaatctaaaa ttctccataa ttcaaacttg gattgataca
    12541 aaatatggtg caattgaact ttggaataat ctaaaacaat ggtttttcca aacggttaat
    12601 aatatcgtgc aaactcttat aaaaagttgg aacagcttaa agcaaggaac gatagattta
    12661 ttcaacaata cagttcaagg tgctaaagac atttggactt cattcaaatc ttggattggt
    12721 gatttaatta ctggaaccaa agataacatc attcagggat gggaaaacct aaaacaaggt
    12781 actatagata ctttcaacaa tttagtaaat ggtgctcaag aggcatggga taatttagta
    12841 aatgctgtta gtgacacggt tgatagagta actggctggt ttgataactt gaaaaatatc
    12901 gatttactag cagccggaaa agctatcatg gatagttttc tagaagggtt acaaaatgca
    12961 tggaaatctg tgcaagattt tgttggaggt attggtgatt ggattcgtga acacaaagga
    13021 cctatccaat acgatagaaa gctattgatt ccagctggtc aggctattat gaacggtctg
    13081 aataaaggtc tgacaggagg attcaatgac gtacagaata ctgttggaag tatggcagac
    13141 tttatcgcgg aacttttcaa tgcaaattct gatgtagata tagctgcaaa tttgaaaaat
    13201 gcaaataaaa acattggtgc acaagttgaa cataaaataa atatgggtgg ctctactaaa
    13261 ccagctgtat ttaaaatcaa tcttggaaga caatcgttta gattgtttat ggacgatatt
    13321 tcacaagcta tgggcgaagg tgcagatatt aatctggaat tttaggaggg aatattttgg
    13381 atcaacgaga aaataaaatg tactcattca aagatacaac cattaatctc actagttcta
    13441 aacgattcct tccgacgtct gccatgatgt acgatggaat gtatttagaa gatttgattg
    13501 atggttatca aacacttacg gtggaaggta gagaaatgct ttctgtagaa gttgaacagc
    13561 aagagataca aattggttca atcattacaa atcagaaaat accttcaaga acactaaaaa
    13621 taacatataa gctggaagat agagatccag aaaaactaca gtttaaattc aaagaactgt
    13681 tgaattattt ataccggaat gaagacgtgg aaattaggtt tcatgatgaa ttagattatt
    13741 attactacgg tcgctataca tcaactgata ctgttccagg agactccaac tcgattattt
    13801 cgagttttaa tgtattctgt gcggatccac taaagtatac gaaagagtgt gttagtgatg
    13861 gctatattgg aaatccgata cagtttccta taacaccaag aaaaattgaa gttactttat
    13921 ccatgaataa ttcaatcaaa attacaaacg gagaacaaaa tatcacgata actgacgcgg
    13981 caataaaaac aggagacgtg ttggtttttg atttttccga tgagcaggta actgtaaacg
    14041 gagaagattg tacttctatg attgatttag aaagtgattt tgagaacttt tatcttaagc
    14101 aaggtcagaa gataactagc aataatggga agcttaaaat attctatagg ggggcgacaa
    14161 tttgagtgag acagtttatt tctttgatca cttgcaaaaa cttattaaaa gaaaaaatac
    14221 aagaagtttg attgaagtct cccaagaaaa agaaattagt tctgataaga gtgatctaat
    14281 gaaagatact ctttacgtta cgacaaaata tgataaagaa atagaggatg caagatatat
    14341 ggcgattcgt gaaaacgagt cgtctttttc gttgtatcga attactaaag ttagcgaccc
    14401 atttgaaaca ttagagttta cagggttagg atttgcgaca aatgaattag atgcttacat
    14461 catcaaagat attaggccga gtgggcagcc cttaaaaaat gttcttgatc gattgattga
    14521 atttactgaa ggaaattggc gcgttggtca cgtagaagca atgttaccaa cagtaactgc
    14581 aactttttac tatgtctctg taaaagaagc gttgaaagaa ttgcaaacct taggtatgga
    14641 atttgtcttt aggtgttctt tgaattctga tggaataaag gataaatgga tcgaagtata
    14701 tgaacaaatt ggcgaagaat cgaatacacg tttcgtatat ggtagtaaag cattaacagt
    14761 tgtaagagag atagatagaa gctcaatctc aacttcaatg ataggtcgtg ggcgaggcga
    14821 agaggttggt gacggttacg gtagaagaat tgaattcact gatgttgaat ggaaaaagtc
    14881 gaatggtgat cctttagata agcctaaagg ccaaaattgg cttgaagatc cggaatcaac
    14941 tcaaaagtat gggataccac aaaaagatgg atcaatgaga aaacgagaaa ccgtagtagt
    15001 gtttgatgat atagatgatc caacagattt acttaaaaat acttattcaa ccttaatcga
    15061 ttctgctaga ccgttagtac aattcaaagc tgaagtcact ggaggagatg tgataggaaa
    15121 tacagtgact attcacagat acgataaagg ttatcactat aaaactcgta tttataaaac
    15181 tacattcaat cggcttaccg gtcaaacgaa tatcgaacta ggggataatt taacacaaga
    15241 tgttagaaaa caaacggctt ctattgtcaa taatattaat agtttagaat ctagcaaaat
    15301 gacattttac gaatcgacag agattggaaa atatcaagat gacattatgc gaggtgcagg
    15361 agataatggc ggttctattt attgggtaaa tggaattgaa gctggtgtta gtgatagtag
    15421 agaaatctat gaaactgttt atatggatgg acctaacatt cctagatcac gcttttttat
    15481 ggtccaaaat aactcaggga tatctttcaa acaatgtaaa aaaggtgagt ggcaaacaat
    15541 tcaagatgta cacaatggtg atagcacgac cgcttggacg ttggatggaa ctttcaatgc
    15601 taattttatt cgcgcaggca ttctgtctgg tatcctcgtg caaggggtag ctttaaaaac
    15661 atttgacgat aaagatttcc aattagtggc agagggggga gaactttctt ttgaaaaaca
    15721 agtcaagtct actggactag atgatgtaca tggagaacgc ttgggctcaa ttgtatctac
    15781 ttatggttct aaagggatta acggctttgc tgtctggaaa gagccaaact atattttttc
    15841 cattaatgct ggggacggtg gcgatcgagg gaatcctatt tttcaaattc cagcagatgt
    15901 tactgctgat aagcgcaaat ataatcttta tggtgacggc aatttttcag aaggaaatat
    15961 aaccatagat ggacggctag atgtaaaaga actctatgtt aacggtacaa aaattgacac
    16021 taatggagga ggcaattccg gagggaacga taacggttgg aacggacaat acccaccaga
    16081 agtaactact gatcgggata aacgatattg gcaaatttgg gcaacggcaa taggtgccgg
    16141 ttttacacaa caagctgctg cagccttact tggaaatgca caaggagaat cagatgctaa
    16201 tccaaccgct gatgagggca atggcgcacc agggttcggt tatggtgtat ggcaatggac
    16261 cgattcttct ggcgcaacta gcggacgtgt ctacatgatc aatttaatga caaaggctgg
    16321 catcagtgat gatccagaca caatcacggc acagtttaaa ttgttgatgt ggcatgcacc
    16381 aaatggtcaa tggatcgcaa ctagcgctta tccttacaca tggacacaat tcatgaatct
    16441 gaccgatatc aacacagcag cacaagcatt cgtggctaac tttgaacgtc cacgtgatcc
    16501 acatccagaa cggacggcat ggtcacaaga atggtatgac aagttcaaag atttggaaat
    16561 tcctgtatca aaaggatata tgaagccgat tgcggatcca atcacagtga cgagcgaatt
    16621 tggctggcgc acttctccaa tcacaggagc acaagaattt cataacggta ttgaccttgt
    16681 aaatggaaat cctaatacac ctatttttgc ttcagcggat ggcgaagtga ttgttgcagg
    16741 tgatgcaaat tactttgact ggtatggaaa ttggacagtg attaaacacg ctgatggaat
    16801 gtatacagga tacgcgcatc aaagccgagt agacgtctca aaaggtcaga aggtaactgc
    16861 tggtcagcaa attggactga tggggacaac aggaccatcc actggagaac atcttcattt
    16921 tcaatttatg gatgagtttt atccatcttc ttcaggtcat ttccacaatg caagagacta
    16981 catcgatttc taaaggaggg atagtcatgg cagaaacgca gcataaaatg gtcctatcca
    17041 ccaccgaacc aaacaacgga ataaatttgg ttcgaattcg gcaaggggat gttttaacgc
    17101 aaaagttcgt tgttgaagtg gtggaacatg gcaaactaaa aacattcgag ggcctagtgc
    17161 cgttttttat taatacaaca aaatttggcg aaaaccaacc tgttgaacaa aaagtacaag
    17221 aatacagtcc agcgcaagca aggcttgttt acaccttaag tgagcctgac tggcaatggg
    17281 gtggtgaaaa caccgcacat ttcagtttcc gatcacttaa tggtgatgga acttggagtg
    17341 aacaatttag cacacaggat tttacctatc gagtcatttc tggaatatct agaagccagt
    17401 tacgtgactc tggctatgtg tggacatttg aggatttgct aagaaaattc aaagattaca
    17461 tggatcaggg caaaaatgac tgggagcagt ggttagaaga taatcgtgaa atactggaaa
    17521 atatcgatcc aggtggtatg atcattaaca ttctgaatga agctaaaggc gactacgaat
    17581 cattagctga tcgtttaaac caaaaatacc aagtgccagt cggcagctca caaattagag
    17641 aaacaacacg cttttttgat tacgacacga tgaaatacgt tgacctagtg ccgcgcaatt
    17701 tgaatacggt tgtcaacagt gttaataaca gcaaatttaa cttttctttc attacagaca
    17761 ttcacgcaga taatcacaac ttgcgtatag atggcgtcgg ttacaaagat gcttcttatt
    17821 taagacattg gcgcgcaatc cctcaatttc aaaaattagg gaacaaaaca gatgtgatga
    17881 tttacggcgg agacaatatc gacggcggac ttggttcact cggcagcgat ataggcatta
    17941 ttgatgaatg gagcgcgcga cattccatgt taggcacgct caaacgcttc actaacgcag
    18001 cggtagcagg acaagaaaaa ccggttatta tctgcaaagg gaatcacgac gcttgtttcg
    18061 accccgcgtg gcgcaagcga aagggaatgt tatgcaacgc tgacttcgaa gagtattgga
    18121 acggtttgta cggtggcgtg ttgttcccag ataaaaacgc agcaatttac cgtttcgata
    18181 cttgtgattt ttatgaaggc ggcacaggcg acaagtacgt ggacggttac agcgatacag
    18241 cgccgggagc tttcagtgcc aaacaaatta acgctttcgg agaatggtta gtgaacgttc
    18301 cgagaaacta tcatgtggcg ttagtaggac atacgccttt aggtctcagc aagtttcccg
    18361 ttcgcaatga aaacatgatt agcacgttga tagaagggtt taaatctggt agtccggtaa
    18421 ctatcgattg gtctaaatta ggacaaccga atgacggttc gttcggcgga ttaaagacct
    18481 tcgcaatgaa tacaaaaggc gccggcgttg tagtaggtta tttctgcggg cattggcacg
    18541 aacaagtcga aggggcgttt ggaacagtaa aaatgattct ttgcgacgtc ggcttctctc
    18601 atacagctag ccaagttgat acaccggatg aattagcgtt ttataaaata gaagttgaca
    18661 cagcaacaag aaaagtgaca agcaaaggcg tagggcgcgc gagagacttc acttataact
    18721 attaaggcgg tgaacaatta aatgttagat tttcaaagca aaccaaatat ttttgaagaa
    18781 atgagttatg aagaggctgt gaaatggtta ttgcgtcaag cggctatcca ttacgacggc
    18841 tcggatcacg acgcgcacgt cctagctact gaaagcaacg cgggctttgc tacaccggaa
    18901 acagttatgc aagcgcgtgg gcgttggtta cgcgattata agctaccaca aaaatatccg
    18961 aacattttag acattccgcc cggcaaatac gcaaccaaag ccggatgggg cgcagacaac
    19021 cctgacggga ttgaagatga cagttttgtt gaaatgatgg tattcgcgga tcacgattcg
    19081 agaaaattaa tcgtcgcttt tgctcgttat agcggcgaaa tttacatcaa aatgacacat
    19141 aacagcgaac cggtagaggg ttataactcg ttaggctggc ggcgcgttta cactacttct
    19201 gttctttttg aaggcgaatt aagaaaagga caatcagtca atctgccaga tgatactttc
    19261 cgttaccaaa cgcttcgtat ccactacaca gacggtgacg gagactttgt agaagaagtg
    19321 aaacgtcaaa gatacgcgcg aattacaaaa gcgaatttat ggaatggaga cgcgggagtg
    19381 acgttcatcg aatttgagct gacaatcgaa gcgcgaaaga taacgatgtc aaacggcaga
    19441 gctttagata tttcatccgg caatgtttcc aatcccgcaa tgagcaacaa cgtgaaaata
    19501 actaagattg aaggtgtgaa ataatggcgc atgtcatcaa aaaaggctct atcaaagtac
    19561 ctacacagcc gaaagactac gatttgcaag caacggggct tgtatttaaa tcatacgata
    19621 atcaaatagc gttagagttc aacgtcgaac aacagaacgg cacaccggcg gacttgctag
    19681 gagctaactt gcgcttgttg atgtttatct atgatgaagt agatggaatg atcacgaaag
    19741 agccaatccc ttttatcacg aaaaacctca tcactgaaag cttcttgaat ggacaggtcg
    19801 tatatatctt gccagaagcg atgaaagctt ataacggtat ggtggaagct tatgtttaca
    19861 tcgagtatcc agacggatca acaagtgata acttaggctt caccttccgt atgaagcgtt
    19921 cagcaatcga cggactagcg caagataaag cagactactt tattgcagac tttcaacaat
    19981 tacttgatgg agtcaaacaa gaagcaacag atgctgttaa cgcagcatta acaaatgtgg
    20041 attctacgat tgaaaaggca aaccaacaaa taaatgaatt tgtagaggga gccacacaag
    20101 caattgatca aactgttgac gaggtaacag agcaactaca agctactcaa actaagattg
    20161 ataccgtttc tcaaaacgtt acatcggcac aaaacaatct taaagcagtt gaagacaaga
    20221 tgaatcaaac caatcagcaa atcggcgacc tcggcaagct gaaaaagatg tacagtaaca
    20281 gcatcgactt cgggaattat gattatagtg ggaatccgaa tttaatgtcc aaactaaaat
    20341 cgagcgattt taacgttggt taccacgggt cactaacttc ggataacgaa aagctacatt
    20401 ttacttctga tggtacagga agcattatta tgtttacgcg tattaataca cctcagcttg
    20461 ctagtgggaa aacctatact ctgagtgcga aagttcgatt tgatgaagga actacaggag
    20521 ctattgataa attacgtttg gtgtatcgta catcaccagg agaaaagata ttattggaag
    20581 caaatagtac aaatattaca acagatgatg tagggaaaga aataacaatc aaaggtacag
    20641 ctaacgttaa ttatcaaatc acaaatttag atcgatttta tatgagtatt agctttgttg
    20701 acagggataa aataaatggc ggatttaagt tgtacgacat caaaat
//
LOCUS       DAYYAJ010000042        20512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000042 DAYYAJ010000000
VERSION     DAYYAJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.042"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..899
                     /locus_tag="ACHATZ_001313"
     CDS             210..899
                     /locus_tag="ACHATZ_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8403855.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            886..2094
                     /locus_tag="ACHATZ_001314"
     CDS             886..2094
                     /locus_tag="ACHATZ_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8403856.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2388..3659
                     /gene="serS"
                     /locus_tag="ACHATZ_001315"
     CDS             2388..3659
                     /gene="serS"
                     /locus_tag="ACHATZ_001315"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8403857.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4208..5692)
                     /gene="guaB"
                     /locus_tag="ACHATZ_001316"
     CDS             complement(4208..5692)
                     /gene="guaB"
                     /locus_tag="ACHATZ_001316"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8403858.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6197..6892)
                     /locus_tag="ACHATZ_001317"
     CDS             complement(6197..6892)
                     /locus_tag="ACHATZ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8403859.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(6982..8082)
                     /gene="ychF"
                     /locus_tag="ACHATZ_001318"
     CDS             complement(6982..8082)
                     /gene="ychF"
                     /locus_tag="ACHATZ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8403860.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8091..8282)
                     /locus_tag="ACHATZ_001319"
     CDS             complement(8091..8282)
                     /locus_tag="ACHATZ_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8403861.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8303..9193)
                     /locus_tag="ACHATZ_001320"
     CDS             complement(8303..9193)
                     /locus_tag="ACHATZ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8403862.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9180..9947)
                     /locus_tag="ACHATZ_001321"
     CDS             complement(9180..9947)
                     /locus_tag="ACHATZ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8403863.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(9959..10672)
                     /gene="rsmG"
                     /locus_tag="ACHATZ_001322"
     CDS             complement(9959..10672)
                     /gene="rsmG"
                     /locus_tag="ACHATZ_001322"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8403864.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10904..12805)
                     /gene="mnmG"
                     /locus_tag="ACHATZ_001323"
     CDS             complement(10904..12805)
                     /gene="mnmG"
                     /locus_tag="ACHATZ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8403865.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12822..14219)
                     /gene="mnmE"
                     /locus_tag="ACHATZ_001324"
     CDS             complement(12822..14219)
                     /gene="mnmE"
                     /locus_tag="ACHATZ_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8403866.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14518..14901
                     /locus_tag="ACHATZ_001325"
     CDS             14518..14901
                     /locus_tag="ACHATZ_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403867.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15069..15827)
                     /gene="jag"
                     /locus_tag="ACHATZ_001326"
     CDS             complement(15069..15827)
                     /gene="jag"
                     /locus_tag="ACHATZ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8403868.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15845..16663)
                     /locus_tag="ACHATZ_001327"
     CDS             complement(15845..16663)
                     /locus_tag="ACHATZ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8403869.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16660..17016)
                     /gene="rnpA"
                     /locus_tag="ACHATZ_001328"
     CDS             complement(16660..17016)
                     /gene="rnpA"
                     /locus_tag="ACHATZ_001328"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8403870.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17134..17268)
                     /gene="rpmH"
                     /locus_tag="ACHATZ_001329"
     CDS             complement(17134..17268)
                     /gene="rpmH"
                     /locus_tag="ACHATZ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8403871.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17791..19125
                     /gene="dnaA"
                     /locus_tag="ACHATZ_001330"
     CDS             17791..19125
                     /gene="dnaA"
                     /locus_tag="ACHATZ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8403872.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19323..20453
                     /gene="dnaN"
                     /locus_tag="ACHATZ_001331"
     CDS             19323..20453
                     /gene="dnaN"
                     /locus_tag="ACHATZ_001331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8403873.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5862 a         4192 c         3781 g         6677 t
ORIGIN      
        1 ggttagttct ggtccttttt cgttcgtctt tataactccg agagacgtaa aatccttttg
       61 ttttcagttt aatgttcatg ttcttgtctt attcacacca actagatgtc tcaccttcta
      121 aaattttcct taacaaagtg ctgcttttat ctatttctgc ttttttttgc tagaataata
      181 gtaacaatat gactctggag gatttattta tgaaaatttt gattgctgac gatgataaag
      241 aaatcgttga attattaagt atctatgttc acaatgaggg gtatgaagca gtaaaggctt
      301 atgatgggaa agaagcatta tctaaaatca gaacaatccc tgatattgaa ttacttattt
      361 tagatatcat gatgcctgag atggatggga tgcaagtagt gaaagagcta cgaaaagaat
      421 cccaaatacc aatcattatg ctgacagcaa aaactactga tatggataag atcaaaggac
      481 tggtcgctgg tgcagatgat tatgtgacca agccatttaa tccgttagaa gtaatggcta
      541 gagtaaagtc tttattacgg cgtacaaaaa tgcagattgc cccagatcag cctgatgtat
      601 tagaagtaaa tgcattaatc atcaataaag actctcatga agttaaaaca atcgatggga
      661 aagaaatcca gttaacagct ttggaattcg gtatcctcta cttgcttgct agtcatccta
      721 atcgagtatt tagcgcggat gaaatttttg agcgtgtttg gaaacaagaa agtattgtct
      781 ctgctaaaac tgtcatggtt cacgttagtc atttaagaga taagattgaa gaagcaactg
      841 gtggtgaaaa agtcattcaa accgtttggg gagtgggcta taaaattgac gcaagatagt
      901 aaacgagcaa ctgaaaaacg ccgtcggatc aatttgactt caaaagaaat cagcgaattg
      961 ttagcggaag gaatcatcac gattatcctt ttgcttctat taaatgtttc tattctggtt
     1021 gttatcagtt cggtgatcaa cagttcacct tctttgacga atgcgatttg ggattcaaag
     1081 aatatttttg cagaacgatt gaacactgat ttgttctgga acggccggaa ttttattatt
     1141 ccctttttct ttctattaga tattggtgtt ttgtattggc gcttgattcg aagatatcgc
     1201 caaatgcaat tgcggcatat catcagcgaa cttcactaca tcgcaaacgg aaattatgat
     1261 catcgtatcc cttttgaact gagtggtgac ttaagccgag tggtaacaag tatcaatgga
     1321 ttggtggata gtaccgtagc agcgattgaa gacgaacgta aaatcgaaaa atcaaaagat
     1381 gaattgatca cgaatgtcag tcatgatatc cgtaccccac tcacgtccat cattggatac
     1441 ctaggactga tagaagatgg ccagtatcac tcagaagaag atcttttaaa gtatacgcat
     1501 actgcttata tcaaagcaaa gcagatgaaa tctttagtgg acgacttatt tgaatacact
     1561 aaggtacgcc agcctgctgt cccggtgaat ttttctgcct ttgacatgat ccagttgatc
     1621 gaacagttag cagctgattt tgagttggaa gcttctaaaa aaaatatcca aatacttgtt
     1681 caatcaaaag tagactccct tatcatggat ggcgataccg aaaaacttgt acgtgtcttc
     1741 aataatcttt tgacgaatgc gttgaaatac ggaaaaggag caacgaagat cgtaatcgaa
     1801 gtagaacgta tcggttcaga agttgttgca acagtcaaaa ataacggagc aatgatccca
     1861 caacaggcaa tcgataatct attcgaccgt ttttatcgtg tagaagaatc tcgttcacaa
     1921 gcaactggag gaactggact tggtttagca atcgctcaaa gcattgtggc tttgcacggc
     1981 ggatacattt atgcaaaatc agataagcaa tggacttctt tcatcatgca ccttccaatc
     2041 aagaaaaatg aaaaattacc tattgataca caggaagtta ttgacgaaag ctgaaatatt
     2101 cgctattctt attacaatta ttagaataaa tgaaaaacac agaacccagt acactttatt
     2161 ctttttcaga gagctgatgg caggtgtaaa tcagcaaagg ataatttgga agttccagtt
     2221 cgtttacgtg ccctttcgaa ataagaaagg tcggttcaac agccgttatg tgttcaacaa
     2281 gtgcagctga cgctgaactt gggtggtacc gcgaatttca gactgattcg tcccaagaat
     2341 aatgggatgg atcagtcttt ttttattttc aaaaaggagg aaatcatatg ttagatgtaa
     2401 aaatgatgcg acaaaatatt gaagaagtac aagaaaaatt gatgactcgg ggcgtaaaaa
     2461 aagaaacatt agctgaattc atggaactag atgaaaatcg tcgtaagcta ctagtcaaaa
     2521 gtgaagaatt aaaaaaatat cgaaatgatg tttctgctga gatcgcccaa ttgaagcgtg
     2581 caaaagaaga tgcttctgct aaaatcgctg aaatgaaaga agtcggtggc aacatcaaag
     2641 cattggatac tgaaattgct gaagtggatg aaaaattgaa acaaattgca actactttgc
     2701 caaaccttcc ccatgattct gtgccagttg gaacagatga agaggacaat gtagaaatcc
     2761 gtcgttggag cgaaccacgt acatttgctt ttgaaccaaa accacactgg gaaattgcag
     2821 aaaatcttga tattttagat tttgaacgtg gcgcaaaagt agccggtagc cgttttgtct
     2881 attacaaagg attaggtgct cgattagaac gtgctgtcta caacttcatg cttgatcagc
     2941 atatttacga acacggctat actgaaatga tcactcctta tatcgtcaac agtaaagcga
     3001 tgtttggtac gggacaattt ccaaaattca aagaagatgt tttccaattg gagaatactg
     3061 atctaacatt aatcccaaca gctgaagtac cattaactaa ctattataat gatgaaattt
     3121 tagatggtgc agatttacct atttatttca ctgcactaag ccctgctttt cgttctgaag
     3181 ccggaagtgc tggtcgggat acacgaggac tgattcgtct tcaccaattc aataaagtag
     3241 aaatggtcaa attttcggat gtagagcatt cttacgaaga attggaaaaa atgacagctg
     3301 atgcagaaga tatcttgaaa aaattgcatt tgccttatcg tgtgatggcc ttatcaactg
     3361 gagacatggg cttttctgca gctaaaactt atgacttaga agtatggata cctgcacaag
     3421 atacttatcg tgaaatcagt tcttgttcta actgtgaaga tttccaagca cgccgcgcaa
     3481 tgatccgtta tcgcgataat gatggaaaaa tccactatac tcatactctg aatggttcag
     3541 gacttgcagt tggccgtaca gtagctgcca ttttggaaaa ctaccaaaat gaagatggtt
     3601 ctgtcacagt tccagaagcg ctcgttcctt atatggggaa tctgaaagtt attaaataaa
     3661 gaggctgtga aaaagccgct tagtttcgag tattaagaaa aattttgtga aaatggcttt
     3721 ccacattttt gcataaattg gacttaatac cgagaaactg gcttttgaac accatttatc
     3781 gagaggttgt gaaatcagcg aattaacctg agtattagag aaaaaaatgg aaaaatagtc
     3841 cctcatattt taccgtttgt tgaactaata ccgagggtca gctgattgaa caccgtttag
     3901 tggagaggtc gtaagcctac cccccagctc ctagttacaa ggacaaattc aaaaaacagc
     3961 tcttcatatt ttatgaatgt tgtacttgtg attcggaggt ggcaagggaa cactgtttat
     4021 tcaaaaagag gctgggacag aagcgtttaa ctccaagaaa taagaagaaa ttcacgaaaa
     4081 ttgcttttca aatttttgtg aatttcttct tatttccgaa ggagttgctt ctacttccgc
     4141 cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
     4201 aatgttttta agattcaact gaatagtttg gtgcttcttt tgtgatttgt acatcatgag
     4261 ggtgtgattc ttttaatcca ctgccgctca tttggataaa ttgcgcttca tcacgtaatt
     4321 gctgtagatt ggctgcccca acataaccca ttcctgaacg caaaccgccg atcatttgga
     4381 aaataatatc tgcaacactt cctttataag caacccgacc ttcgattcct tctggtacta
     4441 atttatttgc ttcattgaca ctaccttgga agtaacggtc ttttgaacct ttttccatcg
     4501 cgcctaatga gcccatacct cggtatgttt tgaatcgacg tccttggaag atttcgaatt
     4561 cacctggtga ttcatctgtc cctgccaaca tacttcctag catcactgca tgtccgccgg
     4621 cagctaatgc tttgacgatg tcaccagaat atttgatccc tccgtctgca ataatcgctt
     4681 tgccatattg acgagcaact gatgctgcat cataaatagc tgtgagctga ggtacaccta
     4741 caccggctac cacacgtgtc gtacaaatcg atcctggtcc gataccaact tttacgacat
     4801 ctacacctgc atcatataaa gcttttgtcg cttcagccgt cgcaacgttt cctgcaatca
     4861 atgttgcatc tgcaaaagtt gaacggattt cttgaatttt tcggatcaca cctgcactat
     4921 gtccatgagc tgtatcgatg atgattgcat ctgcccctgc ttctaataaa gcattcgcac
     4981 gctcaaatgt atcgcttgtt acaccaacgg ctgctgctac aagcaatcga ccatgttcat
     5041 cttttgctgc atttggaaat tcgatcactt tctcgatgtc tttgatcgtg atcaatccgc
     5101 ttaaacgacc ttcgttatct acgattggta atttttcgat tttatgtttt tgcaaaatct
     5161 tttctgcatc ttttagagag gtacctactg gggcagtcac taagttgtct tttgtcatca
     5221 catcagcaat cggcatgtga taatctgtta caaaacgcat gtcgcggttt gtaattatcc
     5281 ctaccaattt tctgttttca agtgtttcaa cgattggcac accactgata cgatatttgc
     5341 tcatcaattc ttctgcgtct gctacaagat gctgtggcgt taagaagaac ggatcaatga
     5401 ttacgccact ttcagaacgt ttgacttttc ttacttcatc cgcttgctgg ctgatcgtca
     5461 tgtttttatg gatcacacct agtccgcctt gtcttgccat agcaatggcc atcttgctgt
     5521 cggtaacagt atccatactt gcactgatga ttggaatatt caacttaata tttttagcta
     5581 attgtacgct catatccact tcgtttggta acacgtgact ttctgctggt atcaataaga
     5641 catcatcaaa agtaaatcct tttttcgtga actttgtttc ccagttggac atttttagta
     5701 ccactcctct aatttagttt atctattaaa ataattgatt ttttcattta agtcaatgat
     5761 ctacttggaa tcaagttagc tttctcatag aataccaata taaagcgttt acatctgtgt
     5821 gtattaaaag agattgtaaa aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa
     5881 aacagcttct catgtttttg aattggttgg gtttattacc gcagatgcaa acttttgaac
     5941 accgtttagt ggagaggctg tgaaaaagct gcttagtttc gagtagtaag aaaaattttg
     6001 tgaaaatggc tttccacatt tttgcataaa ttggacttaa taccgagaaa ctggcttttg
     6061 aacaccgttt atcgtaaaaa gggactgtga caagactttt gtcacagtcc cttactatta
     6121 tttaactatg gataaacaaa atgaggttct ttactacccc tagatatcta gcaaaatagc
     6181 tgatcttttt gctagtttat cttcttggga cggataaact tccaataatc ccgtattttt
     6241 tcttcaagta gtaacggatt gctaaagcag ttgccccgat aatgataact actactggat
     6301 ctaatactgg attgataact ggtggcaata gtgttgtcgc agaaaacagt acgacccaaa
     6361 gaagtgtcgt tccacctagg ataagcaatg tcttacccat tcctggtttc ttgcttcgat
     6421 ctgctccggg atattcatat tgatagatgt atttgtacat catataaaag acccagccac
     6481 cgattgcaga acctagaacg agtgtagaaa agccataagc tggcgttgtt ccacgtgaaa
     6541 acaatccggt aatcgcaatc atgatactta atagaccaag caacagcaac gtattatcta
     6601 accacatcaa taaaggggtg gatgaatttt ttacttctgc tggcttattg atgattgctt
     6661 ccgttctttc agagactgtc ccaaacaatt gacgggctgt tcttccgcct ttttgttctg
     6721 caactaaagt aggcagaatg tcatgaaggg cttttacttt ttcttcttca gacaaattcg
     6781 ctgcttctaa agattttttc aaatcaaaaa tgtactgttg atttcttttg gttaattttg
     6841 tttctaaact attgttttct tttacgattg ctcgtaattg ttctggttcc attatctaat
     6901 agtcctcctt agccgttcct tttctcaacg gtcgtcacaa aagacattct accatattct
     6961 acagctagtt tgatagccct tttacacatt aaagcggaat aacattacgt cgccgtcttg
     7021 tacgacataa tcttttcctt ctaatcgaac acgtccagct tcttttgctg catgcatgtt
     7081 tccgtaatga tccaagtctt caaaagagac agtttctgca cggataaatc cgcgttcgaa
     7141 atctgaatgg atgattcctg ctgcttgtgg tgctttgatt ccttttttga atgtccaagc
     7201 acgtacttct tgttccccag cagtaaagta agtagctaag cctaacaaat cataagcagc
     7261 acggatcaac tgatccaaac ctgattcttc gattcctaaa gcttctaaaa attccgcttt
     7321 gtcttcttca tctaattctg cgatttcttc ttctgcacga gcactgacca cgatcacttc
     7381 tgcattttct tctgcagcaa agttgcgcac ttcttgtaca taatgattcg cgtctgcatc
     7441 tgctacgtca tcttctgaga cattggctac ataaagaatt ggttttgtcg tcaataaaaa
     7501 tagtgattta acgatttttt gttcttcttc agtaaattcg atcgttcggg cagattttcc
     7561 ttcttccagt acaggtttga ttttatccag tacagctagt tctgccactg catctttatc
     7621 tttggtttta gcaacttttg ctacacgagt atagcgtttg ttgatagatt ctaagtctgc
     7681 taacactagt tccaagttga tcgtatcaat atcttctagt ggatcaacac gtccttctac
     7741 gtgagtaata ttatcatcat caaaacagcg aacaacgtga caaatagcgt ctacttggcg
     7801 gatatggctt aagaattgat ttcccaaacc ttctccttta cttgcgcctt ttacgattcc
     7861 agcaatatct gtaaattcaa aagtcgtcgg aacagttttt ttcggatgta caagttctgt
     7921 taagcgttgc aaacgccagt caggtacttc taccatccct acgttggggt cgatcgtcgc
     7981 aaagggatag tttgctgctt ctgctcctgc ttttgtgatc gcattaaaca aagttgattt
     8041 tcctacgttg ggcaacccaa caattccagc agtcaatgcc ataaatttat tcactctttc
     8101 tctgcatttt tttcaattac tttttttaat ttcttttcaa aatctcgacg ggtcatcatg
     8161 acgatatgtc cgcaattcat acatctgatt ttgatatctg cgcccattcg aatgacttcc
     8221 cagcgattcg tctggcaagc gtgaggtttt ttcatttcaa cgatatcgtg caagtcgtac
     8281 ataaaaatag cctccttttt gtttattctt catcaaattg aatattcaag atatcaagaa
     8341 ttcgagtcaa atctttttga gaaagatatt caatctcgat cttacctttc ccattttttt
     8401 cttggatagc tacacttgta ccaaatttat ccatcaaacg atcttcgctt tctcggatat
     8461 aatatggttt ttcctttgct agccttggga tttttttctt ttcttttcca tcattttgat
     8521 ttaactcaga aacaagctgt tccagttggc ggacagttaa attttcagtt actgcacgat
     8581 tggctaattt caagatcaat gttttatttt ttaaacctaa cagcgtacgg gcttgtccca
     8641 tagataaacg ctgatcttgt accatttcct tgacaagatc cggtaaagaa agcaagcgca
     8701 aataattggc gatataaggt ctgctctttc ctaaacgact agctacttct gcttgtgtta
     8761 gtttcaggtt tttcatcagc atctcatatg cttctgcttc ttctaacgga ttcagatctt
     8821 ctcgctgcaa gttttccagc actgctactt gcatcatcgc ttcttcgtca aagtctctaa
     8881 cgatagcagg gatcgtttct ttttcagcta gtttggacgc tcgaaaacgt cgctcccctg
     8941 caataatctc atatcctttt actgcggaac ggcgtacgat gatgggttga aagacacctg
     9001 attgttcaat agaattagct agttcctgca aagatacttc ttcaaatgtt ttacggggct
     9061 gatacggatt tggtcgcagc tcattcaatg gtagctggac aacggtatca tcttttacat
     9121 ccaccgtctc taagttttct aaatcttgaa acagtgcatc gattcctcgg cctaatcctt
     9181 tacttttctt cacgacttac cacttccttt gccaatgctt ggtagacttc agctcctcga
     9241 gaacgtggat cgtagtcgat gatcggtttt ccatgactag gtgcttctga taaacgaata
     9301 tttcttggaa tgatcgtttc gtaaactttt tcgcggaagt atttacgtac ttcttctacg
     9361 acttcatttc ccagatttgt tcgtgcatca tacattgtca gtaagactcc ctcgatctct
     9421 aattctggat taaagtgttt ttggactaaa cgaaccgtat tcagtaattg gcttaatcct
     9481 tccaatgcgt aatactcaca ctgaacaggt atcaaaatcg aatcacttgc tgtaaatgaa
     9541 ttgattgtca gatggccaag agaaggcggg caatcgatca aaatataatc atactgactg
     9601 cttacttctg ctaaagatcc ttttaatctt gattctcttg ccatcattga cgtcaattcg
     9661 atctctgctc ctgctaattg gagagtcgca ggcacaatac tcaaattttc gtgttcagtg
     9721 atcaaagttg cctcatcaat tggcaattca ttgactagaa catcatagat gtctcttgtc
     9781 acatcaggtt ttctgatccc gacaccactg gtagcatttc cttgtgcatc catatccact
     9841 agcaatactt ttttgccaag gctagcaaga caagcaccta gattcaccgt cgtcgttgtt
     9901 ttgccgacgc cacctttttg gttcgcaaca gatattattt gtgccatctc ttctcctcct
     9961 atttgattgg ctgcttatta ggcagtccgg gctttcttgg gtatttcttt ggggttggtt
    10021 tctttttttg aataacgatg atatgccgtt gatcagagac attcggcaag gtaaccgcct
    10081 cttctttgag gaatttacca cctaataaag caatcgcttg ttttgcttct gtcaattcct
    10141 cttcactttt tgctgctttc aatgcataaa aatagccttc ttgtttagcc aagggcaaac
    10201 acaattccgc taacacattc aaacgagcaa ccgcacgagc agtgacaagg tcaaatgatg
    10261 cacgaaagtg aggattttgc ccaaatgttt ctgcccggtc atgatataag gaagcatcga
    10321 ttcctaataa tcgggttaac tcagataaga aggttattcg tttattcaga gaatcaacga
    10381 tcgttatctg cagttctgga aaggcaattt ttaaaggtat gctaggaaac cctgcccccg
    10441 aaccaacatc gcataaagat aaaggttgcg taaagtctac ttcaaatgct aatgtaagtg
    10501 aatcgtaaaa atgcttcaga tatacgtctt tttcatccgt aatagctgta aggttcatct
    10561 tttcgttcca ttcgactaat gtccgataat aaatagcgaa ttgattcatt tgatgatcgg
    10621 taagctctat ccctttttct gccaaggatt ggcgaaattc ttctggtatc ataaatattc
    10681 ctttcattgt gaaacagcaa ttgtttcaca gattctctcc ctactttaac gcaaaatctc
    10741 tagacatgca atccttccat atggattctt aaaaatttga aaagtaagaa aagccgatag
    10801 actttttctt tctagtatga ctcataaaat atacacagaa gagagaacta gctttgagca
    10861 atttctgcaa aaactagttc tctcttctat ttattattac gtattacgct ggttcactga
    10921 ctttcgcaat cttcccttgt tcgatataga ccattaggat acttaggtct gcagggttta
    10981 cgccactgat tcggctagct tgggcaattg tttctggttg gattttctgc aatttttgtt
    11041 ttgcttctgt tgctaatccg ttgattgctg cgtagtcgat gttttctgga atctttttcg
    11101 cttccatgcg ttttaatttt tctacttttt ccatggcttt tttgatgtag ccttcatatt
    11161 tgatttggat ttctacttgt tcgatttctt ttcgtgtcaa ttcttcattt tctggaataa
    11221 accgtagaag ttctgcataa gtaatctcag gacgtcttaa aaagtcgctt gctagtacac
    11281 catcttttaa tggtgccgca ttaatcgttg ctaaaaagtc ttggacttct tctgttggtt
    11341 tgattcgtac attcatcaaa cgtttgattt cttgttcaac tgcttctttt ttcttcaaat
    11401 aattcttgta ttgatcttct tttactaagc ctacacggtg acccatttct gttaatcgca
    11461 aatctgcatt atcatgacgt aaaagcagac ggtattcagc acgtgaagtc agcaaacgat
    11521 aaggttcgtt cgttcctttt gttaccaaat catcgatcat gacaccgatg taaccgtcac
    11581 ttcgtttgat cactaaaggt tcttttccct gaactttcaa agctgcattg ataccggcaa
    11641 tcagcccttg tccagccgct tcttcataac cgcttgtacc atttgtttgt ccagctgtat
    11701 aaagattttc aacgacttta gtttctaatg tcggacggag ctggtgtggc tctactacgt
    11761 catattcgat agcatatcct gtacgcatca tttctacttt ttctaatcca gcaatagaat
    11821 gaagcatgtt gacttgaaca tcctctggta aagaagtaga taaaccttga acatagactt
    11881 cttccgtatt taagccttct ggttccaaaa ataattgatg acgtggtttg tctgcaaaac
    11941 gcacgatctt atcttcgatg gatggacaat agcgagcgcc taccccttca acgatccctg
    12001 taaacattgg ggcacgatgt aagttgtctc gaataatttg gtgagtcgtc tcacctgtat
    12061 aggtcagcca gcaagattcc tgatctagtt tataggcact atctggtgtg ctgaagctaa
    12121 aatgattcgg ctctttgtca ccaggctgct cttctgtaac agaatagtcg atcgtacttg
    12181 attttactcg aggcggtgtt cctgttttga accgttcgat agcaaagcca agttctttta
    12241 gatgattcgc tagtccaata gaaggttgag agttattcgg acctgatgaa tatttcaatt
    12301 caccgataat gatttctccg cgaagtgctg ttccggctgt aataacaact gctttggccc
    12361 ggtagctcgc tcctgtggac gtcactactc catgacaaac accatcttcg acgatcagtt
    12421 cttcgacgat tccttgtctt agcgtcaaat ttggttcttt ttcgatcgta tgtttcattt
    12481 ctgttgaata agcatgtttg tctgcttgtg ctcgcaatgc tcggacagct gggccttttc
    12541 ctgtgttcaa catacgcatt tggatatagg ttttatcgat attcttaccc atttctccgc
    12601 ctaacgcatc gatttctcga acgacaacgc ctttagctgg tccgcctaca gaaggattac
    12661 atggcatgaa tgctaccatg tccaaattta ttgtcagtaa taatgttttt gatcccatac
    12721 gtgcagcagc aagtgcagct tctgaaccgg cgtgtcctgc gccaacaacg atcacgtcat
    12781 acgcatctgc ttcataatgt tccaatcgtt tgttcctcct tttattttcc taaacaaaat
    12841 tgactgaata attgagtgat tagctcatct tgtacactgt cgccgacaac ttctcctaga
    12901 tagtcccagc aacgcgtcat atctatttga acgagatcca caggcattcc tgaatcaata
    12961 cctgcaatga cttcttcaag tgagagggaa gctttttcta acaacgcgat atggcgggta
    13021 ttagaaacat aagtagcgtc tctttctcct gtttctccgc tgaagaataa atcagaaata
    13081 gctgattcca aacgatctag tccgtcgttt ttagctacgg aaattgaaaa aactggttca
    13141 ttttcgatca atttcttcaa tttttcttgt tccagctgtg ctggcaaatc tgttttattt
    13201 agaagaatga tccgtttcaa tccacttgtc gcttccaata attgttcatc ttctgctgtc
    13261 agtggttcgc tttggtttag aacaagtaag atcaagtctg cttctgctaa tgcttttcgg
    13321 cttctttcga ccccgatccg ttccaccaca tcttctgttt cccgaattcc agctgtatcg
    13381 attagcttga gcggtacccc ccgaacattg acatactctt cgattacatc tcgagtcgtt
    13441 cctgcaatat ccgtaacgat tgctttttct tctcttaaca aatggtttaa caaacttgat
    13501 ttgccgacat ttggccggcc gatgattgcc gtacttaacc cttcccgcag aaccttccct
    13561 tgcttggatg tagcaagtag cgcttggatt ctttgttgga tcatctgcgc tttttcaagc
    13621 aataacttcg tagtcaattc ttcgacatca tcatattctg gataatcaat atttacttct
    13681 acttgtgcta atgtctctaa aatctcttgt ctcaatgagc ggatcaaagc agacagattg
    13741 ccgtctaact gattaagagc tagtcccatt gctttgtctg tttttgcccg aatcaaatcc
    13801 atcacagctt ctgcctgtga aaggtcaaca cgaccattca agaatgctcg ttttgtgaat
    13861 tcgcctggct cagctaatct tgcaccttct cttaacaata gctgcaaaat ttgattgact
    13921 acaacgatcc caccgtgaca attgatttct actacatctt cccgcgtaaa tgtctttggt
    13981 gctctcatca cggagaccat gacttcatcg acaagctgat tgctcttggg atcaacgata
    14041 tgtccataat ggatcgtatg acttggtact tctgataatc gtttgtttcc agattgataa
    14101 actttatctg ctagttgcac agcttgatcg ccacttaaac gaacgatact gattgcacct
    14161 tctccaggtg gggtagaaat tgccgcaatc gtatcaaatt ctaaagtaat ttcagccatt
    14221 ttttcacttc ctttttcatt ttgcgcacaa aaaaagtgcc cactccaccc tttatgcaca
    14281 ataaacggtg aacttcgcac tttgactgct tgttcttgat taaattctgg tcatagatta
    14341 tcacacttgc tataaggaat caagtgttta aaacaattag atgaagttga gcctgttctg
    14401 cttccattac cttatattta attgtccttg gatgtctctt tgcgttcttt ggtttattcg
    14461 aagagaagat actgtataaa caaggcttta tctttcttaa aaaaaacggt acactaaatg
    14521 gtagcgctat ctaaactata cacttttgaa aggagaaaat atgtgagccc gttcagatct
    14581 gctgcattgg aagaagaaag tgaccctcgg atcaacgatg tcgttttatc tacagggaat
    14641 atcaaccaga aatatatcgt ccgcgatatc gtttttgttg tggaatgctt agaagctgat
    14701 ttgtttgacc catcttttcc caaagatgac cttttcttaa caacgaagag gaaattaaag
    14761 acgaaggtac ttgaatatgg tgcgaatgca gtaatcaact gtcattttga gtatcagcaa
    14821 aaaaaacgcg acggaaagta ttttctggaa ctttttgctt atggaacagt gatccaattt
    14881 atccaaacaa cgattggtta aatgaaacag acaaaaaaag cggatcaaaa ctgaagacag
    14941 tttcgatccg cttttttcta tcaaagttag tgactacttc atcgtatact ctgataagaa
    15001 tgatttctag taaaatatac ttggtttgaa aaactttgtc aacgtgattt attcaaaaag
    15061 taatggaatc aataatattt tttagcaggt tctactacta aataacgata aggttcctca
    15121 ccttccgaat gtgtttttac atgttcatct ttgcttaatg cagcatggat ttgtttacgt
    15181 tcaaatgcag gcattggttc taagaaaaca ggctgccctg tccgttttac tttttcagcc
    15241 gtgcgatcag ctaaacgctg aatgatctct tggcgctttt cacggtaatt gcctacattg
    15301 actactacag acagcttatt tcttgctaaa cgatggatga agacttgtgc cagatattgc
    15361 aatgcattca gtgtttttcc atgtttccca atcagtatgc cttgcttttg tgtttctaaa
    15421 tggaaaacaa ccatattatt tgattctctt tccattttga caagagctgg agcatcaagc
    15481 tgttttgaaa tttcagtaag ataaactgcc aattccgtta aagctgcttc atcacctagc
    15541 tcacttactt cttctgtttt ttcttcaaga atcgtttcgt tgactacctc gatctcagct
    15601 gttggttctt cttccaaaat ttcttctaca gtctgttcta ctgtttcaga aactttttcg
    15661 ctgatacttg gctggatcga aagccgtgca ttctttcgac ccattcctag gaaacctttt
    15721 tttccttctt ccagtatttc gatttcgact tggtctttgg ccaaacccaa atcagccaga
    15781 cctagttgga cagcttcgtc tattgttgca gcttcataaa tcggcattag cattacctcc
    15841 cctattattt ccgttttttc ttaggattca ttgctttctt aagagcacgt tctctttcgc
    15901 ggattctccg tgcttcttct tcacgctctt ttcgaatttt gaaagggttg ttgatcagca
    15961 gcgtttgtac aacttggaag gcattggaaa ctacccagta taatgaaaga ccgctggcta
    16021 aattgacacc catcactaaa atcattacag gcatcacgaa attcatgatc ttcattgatg
    16081 cattcgattc aatttgactc atgctagata aatacgtact tgcaaaagta aagattgctg
    16141 ctaaaatcgg caaaataaat gttggatccg cgttccctaa gttcaaccat aagaaatgcc
    16201 cttgtttcaa ctctggtaca cgtgaaatag attgccataa cgccatcaag attggcattt
    16261 gtactaataa aggaagacaa ccagcatatg gattgacgcc attttctgca tataaacgct
    16321 gctgctcttc tcttaactta ctttgtgttt ccggatctct tgaactatat tgttgctgca
    16381 gtgcttttaa tttcggttgt aattcttgcg ttttacgcat gctctttgtt tggaaatgca
    16441 tcaacggcaa taggatcacg cggatgatca gtgtaaataa aatgatccct atccctgcat
    16501 taccaaatga caacgccttg atcgcctcag caaagtaata aacaatataa cgatcccaaa
    16561 ttcctgtact ctgtgcgctg acttccccag ttccgcaacc ggataagacg atgaccaaac
    16621 taacgatacc ggccatcaaa agtaaacgtt tgtatttctt cacttactct tttcctctct
    16681 gtcgattatt tttgcaagtt ttaagacgtg gactaaattg gagcgaacct catcataagt
    16741 caaccccttc acacttggtc tagcaatcac aataaaatcc aactccggat caatttcttc
    16801 ttttatagaa taaactgctg ctcggatttt tcgtttgact tcattgcggt ttaccgcgtt
    16861 gccgaccttt tttcctacag ataaaccaac tcgaaaatga ggctgtcccg gtttttccaa
    16921 acggtaaacg acgaatttgc ggttagcaaa agaatctccc gtatggaaca cggcttgaaa
    16981 ctctcgttct ttttttactc gatacgcttt tctcattagt tttctttcct tttctatctc
    17041 tcacttacgc tcataatcat accataatta cgattgtcct ttcatcattt ccctaaaaaa
    17101 gagtataaaa aaaaccactg aaaccccagt ggcctaagct gaaagaactt ttcttccttt
    17161 gcgacgacgg ctagctaaca cacgacgtcc atttttagta ctcatacgtt tacggaatcc
    17221 gtgaactttt tgacgtttac gtttatttgg ttggtatgtt cttttcatat attccacctc
    17281 caaaatgttc ttatctattt tataaacaca gacatacttt gatagtataa cgacaaagta
    17341 gtgattttgt caatataaaa tagaagatcg attgttaaaa cttatccaca agattatttt
    17401 tttctatggg aatcctatgt ggaaaaagtt atgcacaggg attatccaca gataataaaa
    17461 ggctggtttt acacagaaaa cacacgtgta aaaaaagtaa tccacaatca ggcgatttcc
    17521 tgtgtattcc gtctcctaac tattggtata tctgtatttt ttatccacag acgcaagagg
    17581 aaatctattg gtttttacga gttttccacc tgtgcattag tggtgtggaa aacttttttt
    17641 gacaactgtg tttttctctt ttttcttttt gtcgattgtg tggataattg taattaaaaa
    17701 atttttttct tcggtttctg tggaaaacta ttgaaaaaaa tgctaaatta gtactagtta
    17761 ttcacttttt aatgaaggag gaggccttat atggtatccc tcgatgcttt atggaatgaa
    17821 ttaaaagcaa cataccaaaa ggatttatcg cctgctagct ataatacatg gatcgaaaca
    17881 gcgaaccctc gaacccttga tcaaaaccag ctcgttgttg aggttcctag caagattcat
    17941 aaagaatatt gggaaaaaaa cctggcgaca aaaatcgttg aagtcggtta tatgttatca
    18001 ggaaatgaga ttatccctcg ctttattact ggcgaagaag cagagcaaga agaagttata
    18061 gaagaaaaaa atccaaaagt tgtggcacct agtccgctaa aaaaagccat gctgaaccct
    18121 aaatatacct ttgatacgtt tgtcattggt aaaggaaacc agatggctca tgcagcagcg
    18181 ctcgttgtag cagaagatcc aggttctatt tataacccgc tcttctttta tggaggcgtg
    18241 ggacttggta agactcactt gatgcatgca attggtcatc aaatgctgca aagtcagcct
    18301 aatgccaaag taaaatatgt aagcagtgaa acttttgcta atgatttcat caattcgatc
    18361 caaaacaaaa cagcagaaga gttccgtcaa gaatatcgaa atgttgattt actgctagtg
    18421 gatgacattc aattttttgc agaaaaagaa gctacgcaag aagagttttt ccatactttc
    18481 aatgccttgt acaatgaagg gaaacaaatc gtactaacca gtgatcggtt gccaaacgag
    18541 attccgaaac tgcaagaacg ccttgtttct cgctttgcat gggggctgtc tgttgatatt
    18601 acaccgccgg atcttgaaac aagaacagcg attttacgta aaaaagcggc ggctgaacgt
    18661 ctagagatcc cagatgacac actaagctac atcgcaggac aaattgattc taacattcgt
    18721 gaattagaag gtgcgcttgt tcgagtgcaa gcttttgctg cgatgaatag tgaagatatt
    18781 tcgactagtc tagctgcaga cgcactgaaa acgttaaaat caggaaaagg acatcctcaa
    18841 ttgtcaatcc tgcagatcca agaagaagtg gcaaagtatt atcacatcca attgaaagat
    18901 ctcaaaggaa agaaacgcgt caaatctatt gttgttccta gacaaatcgc tatgtatttg
    18961 gcacgtgagc tcacagataa ttctttaccg aaaatcggag ctgaattcgg tggcaaggat
    19021 catacaacgg ttatccatgc tcatgaaaaa atccagcagc tgatggattc tagtgtcagt
    19081 atgcaaaatg aagtatcaga aatcaaaaac tttttactaa attaatttgt cgattctatc
    19141 aactgaaaat agcatgtgga taaaagaaaa aaacgttcaa aagttataca cactttttcc
    19201 acagctgttt tttcgtttct ctcttacgtt ttttgagttt tccacagaat caacatgccc
    19261 tattactttt attattcttt ttaactaata ataaataaac aaacattcaa aggagataca
    19321 tcatgaaagt tactttaaac cgagctagct ttatgcagga attgcaaact gttcaacgag
    19381 ctatttcaag caaaaccacg atccctattt tgacaggtgt aaaaatcaca ctgacacaag
    19441 aaggtttgac tttgacgggg agcaacgctg atatatcaat tgaaactttt ttgtctgttg
    19501 aaaacgaaaa agcaaatatg caaatcgaat ctactggttc cattgtttta caagcacgtt
    19561 tctttagcga aatcattcgg agacttcctg aagaaacatt tactttagaa gttttagaaa
    19621 ataaacaagt agcgatcact tctggaaaag cgaattttat cgtaaatgga ttagatgcag
    19681 ataactatcc tcatcttcct gttgtcgaaa gccataacca gatgaaatta cctgtacacg
    19741 tattgactaa actaatcaac gaaacagttt ttgctgtctc acaacatgag agtcgtccaa
    19801 tcttgacagg tgtgcatttc attttatctg ataattcttt attagctgta gctactgatt
    19861 ctcaccgtct aagtcaacgc gtgattccag tagaacaagc ggctgatcat tttgatattg
    19921 ttattcctgg aaaaagtttg atcgaattat ctcgctcatt aacaaatgaa gaagaaatcg
    19981 tcgaaatcag cattatggaa aaccaagtgt tgttcaaaac agaaacgatg tatttctatt
    20041 cccgtttgct agaaggaaac tatccagata ccaatcgttt gattccttca agttttaata
    20101 cagaggttga attttctgtt cctagctttt tagcggcgat cgaacgggct tctttacttt
    20161 ctcatgaagg ccgtaacaac atcgttcgtt tatctattcg tccagatgcg gttgttttat
    20221 acggaaattc gccagaaatc gggaaagtag aagaaagctt gagttataca gcaagcagtg
    20281 gcgatccatt agatatttct ttcaatccgg attatatgaa agcagcgttg cgtgcatttg
    20341 gagatatgag catcaaagtg aaatttatct ctgctattcg tccatttaca ttggaaccaa
    20401 cggaagatgg cgttcaattt attcagctga ttactcctgt acgtacaaat taacaatgat
    20461 tttcaatgtt cttgaaaatt taattgaaaa gaaatagaaa aaataaaatt tt
//
LOCUS       DAYYAJ010000043        20328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000043 DAYYAJ010000000
VERSION     DAYYAJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.043"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..361
                     /locus_tag="ACHATZ_001332"
     CDS             167..361
                     /locus_tag="ACHATZ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403874.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..929
                     /locus_tag="ACHATZ_001333"
     CDS             630..929
                     /locus_tag="ACHATZ_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403875.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1026..1460)
                     /locus_tag="ACHATZ_001334"
     CDS             complement(1026..1460)
                     /locus_tag="ACHATZ_001334"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8403876.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="ACHATZ_001335"
     CDS             complement(1692..2402)
                     /locus_tag="ACHATZ_001335"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8403877.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="ACHATZ_001336"
     CDS             complement(2392..3252)
                     /locus_tag="ACHATZ_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8403878.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="ACHATZ_001337"
     CDS             complement(3257..3895)
                     /locus_tag="ACHATZ_001337"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8403879.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="ACHATZ_001338"
     CDS             complement(3910..4209)
                     /locus_tag="ACHATZ_001338"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8403880.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="ACHATZ_001339"
     CDS             complement(4252..5736)
                     /locus_tag="ACHATZ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8403881.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="ACHATZ_001340"
     CDS             complement(5756..6217)
                     /locus_tag="ACHATZ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8403882.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="ACHATZ_001341"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="ACHATZ_001341"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8403883.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="ACHATZ_001342"
     CDS             7520..8290
                     /locus_tag="ACHATZ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8403884.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="ACHATZ_001343"
     CDS             complement(8563..9390)
                     /locus_tag="ACHATZ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403885.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="ACHATZ_001344"
     CDS             9958..11193
                     /locus_tag="ACHATZ_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8403886.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="ACHATZ_001345"
     CDS             complement(12192..12452)
                     /locus_tag="ACHATZ_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8403887.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="ACHATZ_001346"
     CDS             complement(12479..13780)
                     /locus_tag="ACHATZ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8403888.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="ACHATZ_001347"
     CDS             complement(14072..14530)
                     /locus_tag="ACHATZ_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8403889.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15244)
                     /locus_tag="ACHATZ_001348"
                     /pseudo
     CDS             complement(14543..15244)
                     /locus_tag="ACHATZ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(15254..16213)
                     /locus_tag="ACHATZ_001349"
     CDS             complement(15254..16213)
                     /locus_tag="ACHATZ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8403890.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(16304..16939)
                     /locus_tag="ACHATZ_001350"
                     /pseudo
     CDS             complement(16304..16939)
                     /locus_tag="ACHATZ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(16950..18392)
                     /locus_tag="ACHATZ_001351"
     CDS             complement(16950..18392)
                     /locus_tag="ACHATZ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8403891.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(18379..19143)
                     /locus_tag="ACHATZ_001352"
     CDS             complement(18379..19143)
                     /locus_tag="ACHATZ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8403892.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(19247..20122)
                     /locus_tag="ACHATZ_001353"
     CDS             complement(19247..20122)
                     /locus_tag="ACHATZ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8403893.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6448 a         4152 c         3135 g         6593 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt caaatgcgtt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacgaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatctcttaa ttgtcaaatt aagttagaca gatcatcaat acttacgtaa ctcaaaaata
    15301 cttccaaagg tgttcgataa tccagtgatt ttctaggaat attatttcgt ttagatgcga
    15361 cagattgaat aaaaaattca tctactgaat tgaaatccat agatttcaat aaaccatctc
    15421 tacgtaacaa tccgttagaa ttctcgttta ggcctctttg agacggcgtt cctggatcag
    15481 cgaaataaat ggcaatatca ttgacattac tgatctgttt ccaatttgaa aattcctttc
    15541 cacaatcaaa agtgatggat ttgaataggt ttttcggtac agattcaaac cattgattta
    15601 attttttttc aatatcaatc gcttgtctac cacacggttt caatgtgatg ataacttttg
    15661 ataatcgttc aactaaggta attacagcac ttttatgttt cagacctacg atagtgtcac
    15721 cttcaaggtg accaaactca tttgagaatt ggctataatc cttttcacgt tcatgaatag
    15781 agcggcggaa agtttgtttt ccacgtcttt cttgatgtcc attcggttta cgcttgcctt
    15841 tcatcggtaa gtcagaagaa tcaaatagcc cctttttgaa catacgataa atggtacgag
    15901 cagaacaact aataggaaac gctgcacgac caacaatcac atcgggtgtc catccttgaa
    15961 caacctttct ttgaatatat tctgattgtt cctcgggtaa aacaagcgga cgtctaccac
    16021 agtttgattt gtttttctta tactgttgat aatattctaa ggctgatttt ccttgcttga
    16081 agaacagata tactttatgg atcgtttgtc ttgaacgatt caagcaatgg gcagttttag
    16141 caacagattg attcattttg aaataagact ctattattac aagttcatcc gttgtaagat
    16201 gggtataggt catttgtact cactccttat aattctttgg tcggaactat tttgagtgta
    16261 gcacaaatga cttttttagt tgtctagctt aattttacaa tcggcgatca taaatcgatg
    16321 tataccagcc aaagtggttt aaaccataat acgtaacgat ccaattctta cgatcataac
    16381 cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat atcacaggca ttaatcattt
    16441 ttacattcgg atatttacga cgaacagctt cagaaacgat cgactctgga tttgtataat
    16501 tcaaaatcca tgctttgggg gcatagtctt gtatatgatc gacaatttcc aataaacctt
    16561 tgattgagcg catcccataa gcaaatcctc cgagtccaca cgtttcttgt cctactaatc
    16621 catgtttaag tggaatcttt tcgtcctttt ctcgcatctc caatcctcca acacgaattt
    16681 gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg atcatccgtt gcacgaatgc
    16741 gaatatttga aaaacctttt cttttcagca taaatttaac aatcaagtac atatccatat
    16801 ttctttcttc atcaatatca tataaacgta tttcagataa tggcaaccgt ttttcattgt
    16861 tcaaaatggc ttgaacgatt cctggtgtat acgtactccc gcctccagct atagtgataa
    16921 tctgtttttt catcatcatc ttttcctttc tattcttcta ataatttatt gaattctgcc
    16981 ataacataag tcacttctaa gccgatgata atttgataat ttttttcagt tggtttaatg
    17041 atcccgctta tccctaattg cttgattcgc tgttcctcga ccatggtggg atccactacc
    17101 tctacccgta ggcgtgtatt acaactatag acatccagga tattggcttt tcctccaaac
    17161 gcttgtagca tttttgttgc catatattca taatttttac ttgctaaccc taactctgtt
    17221 tcttctaagg ttacttcatt gccaaactct gccttagctt cacgtcctaa tgtctgaatg
    17281 tttctcttat tgatcaaata ataaaaagta acgtaataca aggcaaaaaa gaaaattccg
    17341 attggaagaa tcaaaatagc attggtagcg attcgaaaat tgatgagata atcaacaata
    17401 catgctccga atgtaaaacc aattcgtata ttgaataagt aacagacaat ccctgctaaa
    17461 ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa gaaacatgaa actaaattca
    17521 acaggctcag tgatatttgc aataaatgaa gttcctgcac cgcttcccat gattcctttg
    17581 accatctctt tattttcttt ataagctgct ttatagatag ctagacatat agctggtata
    17641 ccaaacatca ttactacaaa aaaacctgat aaaaacagtc cagcctgagg atctccgttg
    17701 ataaagcgtg tcatctctcc tctgaaaact tccttgctag gagatgtata gcttcctaaa
    17761 tcgtaataaa tatacgtatt caacacatgg tgcagtccaa aggggatcag caaacgattc
    17821 aaaaacataa atatccccac acctaatgct cccatatcaa cgagcagttt tgcaaaatta
    17881 tcaatcccaa tttggatgag cggccagata aagcccaaaa aaacagctgc gattgtttgt
    17941 agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa aataagaaaa aactactggt
    18001 aatttttgtt ctttaaagcg attatacgtc catgccgcta ctaaaccgga aataattcca
    18061 ccaaaaatcc ccatattcac ttctgggtcc ataatttcta agcctttttt caaggtagca
    18121 atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct tatttttgac aaaaccgatt
    18181 gcaactccta tagcaaataa gatatccaag tttccaaaaa cggcatttcc agctgcacca
    18241 attactggga tgttcagcat atcgtcagct gataaacgca tgatcaatcc cgctaatggc
    18301 atcgctgcaa ctggaagtag catcgctctt ccaagcttct gcacatttga tttaacattc
    18361 atttttattc ctcctctatt aaatatcctc atatgttact aactcaatca aaggatcatt
    18421 tttcactttt tgagcaaatg ctgaatgaat taaaaattcg acttggtggg tacgaggata
    18481 cacaaaactt gaaccattta acaattcctg atccaaatac gctgcgtgac acatgacttc
    18541 gatcgtttta tcttctctca atatcatgag taaacttgcc aaatattctt ccacactcgt
    18601 attgatttgt cgtttttttt catcagtttc tcctacgaca tcgaaacaag gctcaaaata
    18661 atcgacatga cgaacttcat cttttcgttc aaagttgctc cgaaccggca aatcatattt
    18721 ttttgctaat gtaacaacta cctcctgatt ttcatccaaa gtatgagtat gatgatgact
    18781 gtctaaatga gttggtatga tccccgcatg ataaattttt tgaatctgag cattccattc
    18841 ctgatacagt tgatctttgt caatgttaaa tggatgctga taataagata aggatctgaa
    18901 ctctccgctt tcgataagag tatcgacagt tttgaggagt ggtttgccac acgtcaatgt
    18961 taaatggact cctacaccta gcctaggcat ttcctttctt agtttcacag catgctcgaa
    19021 acccggcata tttgccatca atgtagttga tgtcaaaatt cctcgctgat aagcatccat
    19081 aatcccataa ttcacaccat ggctatagcc aaaatcatcc gaattaaaaa tcacttttcc
    19141 catttcattc tccttctttt ttctactaaa ataggcgcaa ccaaataatc atagtccatt
    19201 tactatgtgt catttggttg cgcctataga tagttacgcc cttattttaa tagattcttg
    19261 ctagatgaat tgccaaatag atgatttcat tttcttgaat ggcaagttga aattgctgtt
    19321 cgatgatatc tttaatcttt agtgctgtac aatacgcttt gctatgttta aatgtttcag
    19381 taaaaaaacc aatattttct actggcgttt cttcctgata aatcaagcgt ttaaccagga
    19441 atttgatatg aaccattaag cgatgataag tcagtgattc tttatcataa acgtttgaat
    19501 aggatttttc tagatgggac aaaatcgtgg tcgtcatttc tactattttt aagctcttta
    19561 aactattagg ctctcctagc tcagcattaa tgatatgcat agtgatgaat cctacttcat
    19621 ttgaccctaa ctcggtttga tatcgatgat tgattcgctc gaccacttct actgctatat
    19681 catattcatt tggataaagg aatctcattt ctggcaacat aattgctgtg acatctctat
    19741 cttctctttt tcgttgaata gcaaaataaa tatgatctgt caatgtaata tagatatttg
    19801 aatctaatgg agaggagatc ctagctgtta tcagttcaat cacttcttcc gcaaaatcca
    19861 taaattcgat tggaatctgc gagactatct cactgaatcg tttgatcttt tctttagaat
    19921 ctaatacaaa taatttgtca gcatcctcaa catcaaatgg cttactcact ttagcagaaa
    19981 agccaatccc tttccccatc gcaatttttt ctattccatc gtcatcgatc aatacagcat
    20041 tatgattcag ctttttcatc acatacaccg ttaattcacc tcctctatat gataaaacaa
    20101 aacaacttgc agactccttc atacatattt ttgtctccaa acaattttta aagacaaaaa
    20161 acgacctaag aaagaaccct ttcttaggtc gtgcctaatt gaatagtagc accctacata
    20221 atttacaaaa atagtttaac acataataaa agcgttatca atatcttttt tactcgtttt
    20281 atgataatac ttgtatcgaa ttgatttgga ggtaattaca taaaaaca
//
LOCUS       DAYYAJ010000044        19631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000044 DAYYAJ010000000
VERSION     DAYYAJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.044"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHATZ_001354"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHATZ_001354"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8403894.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHATZ_001355"
     CDS             complement(1174..1869)
                     /locus_tag="ACHATZ_001355"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8403895.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHATZ_001356"
     CDS             complement(1882..2598)
                     /locus_tag="ACHATZ_001356"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8403896.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHATZ_001357"
     CDS             2928..3536
                     /locus_tag="ACHATZ_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8403897.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHATZ_001358"
     CDS             3861..4184
                     /locus_tag="ACHATZ_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8403898.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHATZ_001359"
     CDS             4366..5736
                     /locus_tag="ACHATZ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8403899.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHATZ_001360"
     CDS             complement(5866..7167)
                     /locus_tag="ACHATZ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8403900.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHATZ_001361"
     CDS             complement(7521..9512)
                     /locus_tag="ACHATZ_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8403901.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHATZ_001362"
     CDS             complement(9656..9922)
                     /locus_tag="ACHATZ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8403902.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHATZ_001363"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHATZ_001363"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8403903.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHATZ_001364"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHATZ_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8403904.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHATZ_001365"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHATZ_001365"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8403905.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHATZ_001366"
     CDS             complement(14082..14603)
                     /locus_tag="ACHATZ_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8403906.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHATZ_001367"
     CDS             complement(14606..15448)
                     /locus_tag="ACHATZ_001367"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8403907.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHATZ_001368"
     CDS             complement(15441..16166)
                     /locus_tag="ACHATZ_001368"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8403908.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHATZ_001369"
     CDS             16342..16638
                     /locus_tag="ACHATZ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403909.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHATZ_001370"
     CDS             17206..18798
                     /locus_tag="ACHATZ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8403910.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHATZ_001371"
     CDS             18811..19551
                     /locus_tag="ACHATZ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8403911.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5618 a         4028 c         3724 g         6261 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt c
//
LOCUS       DAYYAJ010000045        19457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000045 DAYYAJ010000000
VERSION     DAYYAJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.045"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHATZ_001372"
     CDS             133..3660
                     /locus_tag="ACHATZ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8403912.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHATZ_001373"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHATZ_001373"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8403913.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHATZ_001374"
     CDS             7448..7789
                     /locus_tag="ACHATZ_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8403914.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHATZ_001375"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHATZ_001375"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8403915.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHATZ_001376"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHATZ_001376"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8403916.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHATZ_001377"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHATZ_001377"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8403917.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHATZ_001378"
     CDS             13000..13737
                     /locus_tag="ACHATZ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8403918.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHATZ_001379"
     CDS             13751..14566
                     /locus_tag="ACHATZ_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8403919.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHATZ_001380"
     CDS             14579..15226
                     /locus_tag="ACHATZ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8403920.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHATZ_001381"
     CDS             15242..15898
                     /locus_tag="ACHATZ_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8403921.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHATZ_001382"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHATZ_001382"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8403922.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHATZ_001383"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHATZ_001383"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8403923.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHATZ_001384"
     CDS             18240..18758
                     /locus_tag="ACHATZ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8403924.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHATZ_001385"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHATZ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8403925.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DAYYAJ010000046        19205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000046 DAYYAJ010000000
VERSION     DAYYAJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.046"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..2197)
                     /gene="copB"
                     /locus_tag="ACHATZ_001386"
     CDS             complement(26..2197)
                     /gene="copB"
                     /locus_tag="ACHATZ_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8403926.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2217..4403)
                     /locus_tag="ACHATZ_001387"
     CDS             complement(2217..4403)
                     /locus_tag="ACHATZ_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8403927.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="ACHATZ_001388"
     CDS             complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="ACHATZ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8403928.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4625..5065)
                     /locus_tag="ACHATZ_001389"
     CDS             complement(4625..5065)
                     /locus_tag="ACHATZ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8403929.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5139..5637)
                     /locus_tag="ACHATZ_001390"
                     /pseudo
     CDS             complement(5139..5637)
                     /locus_tag="ACHATZ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(5922..7388)
                     /locus_tag="ACHATZ_001391"
     CDS             complement(5922..7388)
                     /locus_tag="ACHATZ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8403930.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(7699..9780)
                     /locus_tag="ACHATZ_001392"
     CDS             complement(7699..9780)
                     /locus_tag="ACHATZ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8403931.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10304..10663)
                     /locus_tag="ACHATZ_001393"
     CDS             complement(10304..10663)
                     /locus_tag="ACHATZ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8403932.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(10693..11034)
                     /locus_tag="ACHATZ_001394"
     CDS             complement(10693..11034)
                     /locus_tag="ACHATZ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403933.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(11031..11705)
                     /locus_tag="ACHATZ_001395"
     CDS             complement(11031..11705)
                     /locus_tag="ACHATZ_001395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8403934.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(12639..13937)
                     /gene="asnS"
                     /locus_tag="ACHATZ_001396"
     CDS             complement(12639..13937)
                     /gene="asnS"
                     /locus_tag="ACHATZ_001396"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8403935.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(13977..15167)
                     /locus_tag="ACHATZ_001397"
     CDS             complement(13977..15167)
                     /locus_tag="ACHATZ_001397"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8403936.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15188..15715)
                     /locus_tag="ACHATZ_001398"
     CDS             complement(15188..15715)
                     /locus_tag="ACHATZ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8403937.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(15762..18551)
                     /locus_tag="ACHATZ_001399"
     CDS             complement(15762..18551)
                     /locus_tag="ACHATZ_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8403938.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(18698..18898)
                     /locus_tag="ACHATZ_001400"
     CDS             complement(18698..18898)
                     /locus_tag="ACHATZ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8403939.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT         5257 a         4177 c         3332 g         6439 t
ORIGIN      
        1 tttttgtcac aacctttttt cattattatt tgattttcaa tgtcaaagca tttagagcta
       61 caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa
      121 ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc
      181 agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat
      241 cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg
      301 caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac
      361 ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat
      421 ctggtaataa accgccataa tattcttcga tccctaaata atctgctact gcttgggcag
      481 cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga
      541 taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat
      601 tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt
      661 gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt
      721 ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag
      781 gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga tgattggcat
      841 tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta
      901 cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga
      961 tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt
     1021 tagctgctat tgatgtggag cgagcaacaa ctaatgggat agctaaacct agagcatgag
     1081 gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga
     1141 acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg
     1201 ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt
     1261 ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc
     1321 catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg
     1381 attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg
     1441 ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg
     1501 aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg
     1561 ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta
     1621 gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg
     1681 gattgagcag atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca
     1741 tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta
     1801 aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag
     1861 aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga
     1921 ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg
     1981 aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt
     2041 ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat
     2101 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     2161 tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     2221 tttttcatgt gtcgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     2281 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     2341 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac
     2401 aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt
     2461 tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc
     2521 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     2581 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     2641 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     2701 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     2761 gccacggcaa tccacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     2821 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     2881 aatcgtttac gtgtacccgc aaaatagcgt actccattaa tcgtaccgct gatcccagca
     2941 cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     3001 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     3061 aggacttctt ttgaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca
     3121 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
     3181 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggggtcgct
     3241 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
     3301 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
     3361 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
     3421 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
     3481 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
     3541 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
     3601 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
     3661 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
     3721 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
     3781 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
     3841 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
     3901 ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct
     3961 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
     4021 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
     4081 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
     4141 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
     4201 taaccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
     4261 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
     4321 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
     4381 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
     4441 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
     4501 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
     4561 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
     4621 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
     4681 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
     4741 tagcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
     4801 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
     4861 atttttttcc tttttgttct gtccagatca cttctttttt taccaaacgt cccagcaaag
     4921 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
     4981 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct
     5041 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
     5101 agcttatgaa tacagattac gcttgtaaac aaaaactgtc aattattttt gctctctatt
     5161 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
     5221 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
     5281 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
     5341 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
     5401 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
     5461 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac tataaacgat
     5521 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
     5581 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
     5641 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
     5701 caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc
     5761 agctgacctt cagcattagc tcaaaaaacg gcaaaatatg aggagctatt tttgtttggc
     5821 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaga
     5881 agtagcttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg aaccactgtt
     5941 tctggttcag taatttcttt gtttttgtct cgacgagcaa ttgctggcaa gatcatacct
     6001 aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca ccatgcttta
     6061 ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa cgcagtgaag
     6121 gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa tttatattca
     6181 gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac ccataggtaa
     6241 cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc attcatatct
     6301 ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt cagaagataa
     6361 ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac aaattctgga
     6421 tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa tgcggagatt
     6481 tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat accatagtaa
     6541 ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt agggatatta
     6601 tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat ccctaccatt
     6661 gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt agagttgaca
     6721 taaggggata ttttttccgc ccctccaact gcaaatacta gcatcgaaac ggttgtgaaa
     6781 taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg tgtggcaaat
     6841 tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa gatcgacatg
     6901 acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag tccttttgtt
     6961 gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat ttgtactgac
     7021 atcgtattta cgatatttcc atttccttgg actgcccaac caccagcaat cagaattgct
     7081 tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc ggcatagtat
     7141 gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc tttactatcc
     7201 ttgaaggctg atcctaattg tccaacgatc aatgcgtatg gaataaaata aatggccaaa
     7261 atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt aactacattc
     7321 cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg taattgtttg
     7381 ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa tcatattcaa
     7441 tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attattttat ttaatacaca
     7501 tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttatt tcattgacct
     7561 tattgaattt caagctattt tgaattttct gcaaaattaa aaacattagt tattttataa
     7621 tcctatctat tttctttaca tttttcatta agaaaaggat ataaaaaaaa gaggacgttc
     7681 attcgtcctc ttcattgatc aacgtttatt ttttacatgc aagcttcgca tggcattcaa
     7741 aacagctaat actgtcaccc caacatctgc aaatactgca tctcccattg atgcaaaacc
     7801 aaaagcacct aaggaaagta ccaaaatttt tatcccaata gcaaagacaa tgttttgttt
     7861 gacgattttc aatgttttct ttgctaaatg gattgcttct gcaatcttcg atggttgatc
     7921 attcatgatg actacatctg ctgcttcaat tgcagcatcg cttcccaatc cacccattgc
     7981 gattcctaca tctgctcttg ctagcaccgg tgcatcgttg attccatctc cgacaaatgc
     8041 ggtcttttta ttgttacttt gtaagatttc ctctaaccgc tgcactttat cttctggaag
     8101 caattcgcta tatatcttgt ccacgcctac ttgcttcccg atatgatcag caattttttt
     8161 cgagtcgccc gtcaacatga ctgtgttttt cacaccagcc tgttttaatt gaaccaatgc
     8221 gtctacggca tcttctttca aggtatctgc aataacaaga taaccactgt aactttgatc
     8281 gattgctaga taaagcaatg taccgatttc ttgacttgaa ggaaaagaga tttcaaatct
     8341 ttccatcaat ttgtgattac ctactaatac ttgtttgcca tcaacttcag ctaagatccc
     8401 ttcacctgca aattcctgga tattagctgt tggtaaaagc ggacctgcgt agccttctaa
     8461 aacagattga gcaatcggat gtgtagaaaa ctgttctgca ctcgcaatat attgcatgaa
     8521 tatctttgga tcgatttttg tatcgatcgt ctggatcgaa aagtcgcctt tcgttaatgt
     8581 gccggtttta tcgaatacca ctgtctcggt ttgcgcaagc aactccaagt aatttgaacc
     8641 ttttactaat acaccgattt tacttgctcc tccgataccg ccaaagaaac taagtggcac
     8701 ggaaatcact aatgcacatg gacatgagat cactaaaaat gtcaatgctc gatagatcca
     8761 ttcagaaaac gccgctccag aaacaacaag tggaggtaaa acagctaata aaacggccaa
     8821 tcctacaacg actggtgtgt aatagcgcgc gaatttagta atgaaatttt cagctggtgc
     8881 ttttttactg ctggcatttt ctactaattc taataatttg ctgatagtag aattttcata
     8941 agatttttct acttgaacta aaaatggttt atttttattg ataaatccgc ttaatactgt
     9001 ttcaccaaca tatacagatt tagggactga ttctcctgta agtgctgacg tatcgactaa
     9061 tgattctcct tcgatgatcg ttccatctaa cggtacacgt tcaccaggct tcacaagtac
     9121 aaactgaccg atcagcacgt cttcagggtt cacggtcatc aatccttccg ccgtctgcac
     9181 gtgagctgtt tctggacgga tcgccatcaa ttcgcgaatc gattttcttg aatgactgac
     9241 tgccaatcct tggaaatact cgccgatttg ataaaatagc atcactgcta cggcttctgg
     9301 atattcaccg atcaacatag cgccaatcgt agcaacagac ataagaaaat tctcatcaaa
     9361 gatttttcct ttgacgatat tcatcactgc cttcttcact acatcatatc ctatcagcag
     9421 ataaatcaat aaatacccta tcaagcgaat tagttcatta aaatcaaata atagcaataa
     9481 agcgatacct gcaagggaaa ataaaatccg gaacagcgta tttctctctt tttcatttcc
     9541 atgttcagat actttttcct tatctatttc tgttactttg acatctggtt ctaattcctt
     9601 cactactttt tttgtttctt gttcaatcgt agccaaatga tcttcctccg cgtcaatcgt
     9661 tagttttcca gaagtgaagt ttaccgttgc ttccttgact ccattgattt tctgaactcc
     9721 tttttcgatt ttcattgcac agttcgcaca atctaaacct tctaatctgt aacttttcat
     9781 tttaatgacc tcctcgcctc aacatatgaa tatctcttca taactaatta taaatgaatg
     9841 gttattcata tgtcaaggta tccgaaagaa aaaatgaaaa acatctgcat cggaaaattt
     9901 cttccataca gatgttttgg cttattcttt ctttttctct tcgatcattg ccactgctct
     9961 tatccaaccc gcacttgcct tcttgatctt gacaggatag caactaagtt tgaagcctgt
    10021 tgctggcaat ttgtctaaat tcattaattt ttcgatagga caatatcttt tttcaatccc
    10081 tgcaaaatgt ccttcccaga tatacgaagc tccactacgc tttcaaaaat atagaaagag
    10141 ttctttaatg aaaattattt caactttcca taaacagtaa ccccatataa ataaaagacg
    10201 taaaagtcac atggaagaac ttatacagga aaacgcatag agtcaggtga aatacatgta
    10261 tcccacctga ctctatgctt tatctaacct ttattattct cttttataca atttctgcac
    10321 cgaatggcat caacgccaat cgagcaagtt tgaaagattg cttggcaaaa ggtatcccta
    10381 tgatcgtgat acatagaagg atgccgctga cgacatgtgc gagagcaatt ggtaggcctg
    10441 aaacgatcag ccagatgata ttaacgatca atgacatgcc agactggtga taaattactt
    10501 ctttgccaaa aggcgcaaaa gaaaggaagg cgatcttaaa acattgcata ccgattggga
    10561 tacctatgat cgtgatacac caaagtatcc ccgctgctaa ccagctaaga ccaccgatta
    10621 gtccgccgaa tataaaccag atgacatttc ctaaacaact cattgtattt ctccttctat
    10681 tactgttttt gattaaaact gggcaaaaat tgattttatt cttctttttt tagcttccag
    10741 catcaaatat tctgcaaatt ttttttggat ttcttccgta ttttcttttg ttactaatgt
    10801 gtgttggatt agttttcctg atggtcgctg acccacccgt tcttctgact gttgcacgtc
    10861 cgcttgcgca aaaccgataa tccgtttttc ttttgttaca gcaacgagca atagtttatt
    10921 cttatcattt aggtcagctg ccgtttttgc ttttacgaat gattctggat gatcagtaat
    10981 cagctgattg atttgaggta gatcttcttt tactgcccac cgtatttcca tcatttctca
    11041 cttttctttc gctcattttt atctccacta aaaaaatcga ctgcggcaat tgcatctatc
    11101 cagctttgtg caatgatctc atgtccgcca atcgttgggt ggaccccatc ttctcttgta
    11161 tatttcgaaa aaccataagc aatccctaaa gcattgaata tgccatctaa aggaaccagc
    11221 aatgcgccat attccttagc aagtttacga acaatttgga ttttagggtc taagtcttta
    11281 cgccagccat gacggtcgtc ggttatagga agtacaaacg gctccatcaa gatgatgttg
    11341 cagattcccg ctttttgaag ttcatttagt aattttcgat aaacggattc aaattgctga
    11401 gcagatttct cagttccgaa tatagcctga tcgctagtat tatgccaggt atcattgatt
    11461 ccgatcaaaa tcgatacata gtcaggttgt aaagctaaac aatcttctcg ccagcgtact
    11521 tgcagatctg caattttgtc tcctcctatc ccacggttca tgcattcaaa ttccatcgtt
    11581 ggatattggg agattagttt tccggcaatc aaaagtggat aacctttacc tagatcagtt
    11641 gcttctgctc ggtctcttcc tgcgtctgta atgctgtcac cgataaataa tatacgtttt
    11701 ttcatttgtc aaaaactcct ttttgaataa aacgatgatt ttcttcctta tattatccca
    11761 taagccctat caagacgaaa gagaaaatat atgtataaaa aatgacacca gcctagtccc
    11821 ttcaaacaat tgaatgaacg ctcatcaagt gtctagtttt ttatgctatt tttttcttaa
    11881 cgtgctggat tccccttgat tccttcattg tttggtccag agagatcgat cacacctaag
    11941 cttccgtcaa tcagaccact tgatgacgcc atcagaacat atatttatag gctcatctaa
    12001 gacttccagt aaatgttcct ttactaatat ctcttctaga ttgcttggga tagattggcc
    12061 ataactttat ctggtccgat gatttcaagg gtgaattgaa cttacatatg gatcattgca
    12121 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtgct
    12181 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
    12241 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
    12301 atttcttttg ctttcttctc ttatctgctg gaaacttcct ttatcccgta tttaaaaaaa
    12361 aagaactgcg agagttcgca gttctttttt ttaaataaac gttgctctcc ggccaactcc
    12421 ttggtcacaa gtcacaatag tcatcaaaca tgaaaaaatg tttttttgaa attactcctt
    12481 gtgactcgga gctgaacggc agacgcacaa cctcttgata taaacggtgt tcaatcagct
    12541 gaccctcgat attagctcaa aaaacagtaa aatatgagaa gctatttttc cgttttgttt
    12601 ctctaatact caggtcaagg cactgatttc acaacctctt atggataaat tcggttcaac
    12661 aaacgtggga atggaatagc ttcacgcaca tggtcgatac cagcaatcca agtgactgtt
    12721 cgttcaaggc ctagaccaaa accagagtgc ggaacagaac cgtatttacg tagatcaaga
    12781 taccatgaat attctttttc atccaaaccg tgattacgga tttgttctag caagaaatca
    12841 tagtctgttg cccgttcgct tccaccaata atttcaccgt aaccttctgg tgcgatcata
    12901 tctgcacaga tcaccacgtc ttctcttgtt ggatgtggtt tcatgtagaa tggcttgatg
    12961 gcttttggat agtttaggat aaagacaggc tgttcgaatg aattcgcaat gaacgtttca
    13021 tgaggtgaac caaagtcttc gccccattcg atgtcatcaa atccattttt cttcagtaat
    13081 tcgatcgcat catcataagt aatacgaggg aatggcaatt ttgtataagt ttttaagact
    13141 tctttgtctc gtcctagtac atctaatgcg tattcgcaat gatctaaaac attttgtact
    13201 aagaatgcta catattgttc ctgtacttct aaactttctt cttgatgagt gaaagccatt
    13261 tctggttcga tcatccagaa ttcgatcaaa tgacgtcgtg tttttgattt ttcagcacgg
    13321 aaagtcggac cgaatgaaaa gacttttccg aaagccatag cagcagcttc catgtataat
    13381 tggccacttt gagataaata agctttttga tcaaagtaat ttgtttcgaa tagatccgta
    13441 gtgccttctg gtgcagaacc agtcaagatc ggcggatcga ttttgacaaa atgatgttga
    13501 ttaaagaatt catacgttgc acgaatgatt tcatttctga tctgcataat cgcatgttgt
    13561 cttgaagaac gcagccataa atgacgatga tccatcaaga agtctgttcc gtgttctttt
    13621 ggtgtgatcg gataatcgtg gctctcgctt accacttcca catttgtaat tccgatttca
    13681 taaccaaatt ttgaacgact gtcttcacgg atttctccag ttactaacac ggatgtttct
    13741 tggtgtagat ttttagctgt ttggaaaact tcttccggca cttcgctttt tacaactaca
    13801 ccttggaaaa aggctgttcc gtctcttaat tgcaaaaatg cgatttttcc gcttgatcgc
    13861 ttatttgcga cccaagcgcc gattgttact gtttctccca catggttttt cgaatcgatg
    13921 atttgaattt gttccacgta tgactctcct tttatattcc ttgaatttct tcaggattaa
    13981 ttttgacttt tactctcaat aaattgcttg attctgcgta ccgcttcttc taacgtagcg
    14041 cgatcagtgg cataactcat gcggacattt tctggtgccc caaagccttc gccagtcaca
    14101 agcgctaccc ctgtttcttc taaaagatct tctacccatt gtgtgacatt ttcatattta
    14161 cacatgcgta gcgtttcttt tacattagga aaaagataaa aagctccttg tggcttttca
    14221 agagaaacac ccggcagttc tgccacaagc ggatataacg tattcaggcg ttcttcaaag
    14281 gcgttccgca tttcttccac tgtatcttgc tcgcctttca atgcttcaac agctgcatat
    14341 tgactgactg ctgtcggatt actggttgac tgggaagcaa cagcaatcat tccattgatg
    14401 atctcagaat tccccaccgc ataaccaatg cgccagcctg tcatcgcata tgttttagac
    14461 acgccattga tgatgatggt ctgatttttg attgcttctg aaatcgttgc aattggagta
    14521 aactcattgc cattataaac taaacggcca taaatatcat cagcaactat caaaatatca
    14581 ttttttactg cccattcacc gattgcctgc agttcattct tgctataaat cattccagtc
    14641 ggattcgatg gagaattgat gattacggct tttgtttttt ctgaacgtgc ttgttctagc
    14701 tgctcaacag taactttcca gctattggat tcttcccctt tcacaaagac aggaagtcct
    14761 tctgccagtt ttacttgctc accgtaactg acccagtaag gcacaggaat gatgacttca
    14821 tcttgcgggt caaggatcgt ttggaaaagg gtatacaaag caaatttcgc tccgtctgtt
    14881 acgactgttt gtgaaggttc atagacaagt ccatagtttt tcttcagata atcaacgatt
    14941 gcctgtcgaa gctctgggat tcctgctgtt ggtgtataat agctagcttt cccattgcgg
    15001 attgcttcga ttgctgcttc ttggatgttt tctggtgtcg caaaatccgg ttctcccaca
    15061 gtcaaactca gcacatctct gccttttgct tttaatgcct tggcctttgc cgcagctgcc
    15121 aaagtgacag acggttctaa tcgttttgtt ctttttgata actccatccc attcattcct
    15181 ttacctttta aattcccttg attgtcttga cttcatctcc agattcaaag ccaatcaggt
    15241 aataattcag tttcccattt gtttcttttc ctactacttc ccacacaggc tgatcatcat
    15301 acatccctaa agcagctttt tcgatttgga tttcaggatg ggccgcagca attgtctgtt
    15361 ttgcttcgtt ttcagtcagt ccctttgttt gatccaatac ttttactttt tcaccagatt
    15421 taggaatgat aacggcgatt tcttttcctt tatcattcgt acccgtcaat gaaaagtacg
    15481 ttttctttcg agtaaaccag taaaaatgat cggcttcctc gagatttgca tatttctttg
    15541 cgatttctac tgcttctttt ttcgcctgga ccgccggacg actggaacgc aagtaaaaaa
    15601 tcgagctgat cacgatgacc gctaataaaa atacgatcaa gccaagcaac acgcgattaa
    15661 tcgttttttc ctttgtctgt tttttcttca agggtcgtcg ctcctttatc ttcattcctt
    15721 ttcattatag cagattttaa tgatgaaaaa agacggcacc tttaacttcc ttcttcattt
    15781 ttctttaaga attcatggat atcctgatag atttcatcta tagctagctc ttttatcggt
    15841 acttttttcg gtaatgcatg gatgattcgt gcaccatatt ttgcagtgac taatctccga
    15901 tccaaaataa gtaatacgcc acgatctgtc tccgtcctta tcaaacgacc taatgcttgg
    15961 cgtaacttca aagctgcttt aggcacggat tcctgataaa aaggattgat cccctctgat
    16021 tccagataag cattcctagc tttgaccatc ggacgtaacg ggttgtcgaa gggtaaacgt
    16081 gtgactacaa caatctgcaa agcatctcca ggtaaatcta cgccttccca aaagctgtca
    16141 gcaccaaata agacgctttt ttctgacaaa atgaagcgtt tcaaaagttt ttcccgactc
    16201 ccgccaaatc cttgagcaag aatttcacgt cctttttcta gcatagacaa atgcattttc
    16261 tgatatacct tttgcaaaac atcatgagaa gtgaataaaa caaggatcgg ccgatcttct
    16321 tgttcaatca atcctgtgag tgctgcttct aaataatcga tatatgcttg attgctgact
    16381 tgatggactg caggtgcttc tttcggcaca tagattcttg cctgtttctc ataatcataa
    16441 ggagaagcaa tcactttaag aggtgctgct tcaatgccaa gtcgtttaga gagatactgt
    16501 cgattaggtc cgatcttcag tgttcctcca atataaagaa tatggtcata tcgaggatac
    16561 cacacagttg acggcaacat agctgcttga aaatcaagga gatgaaaaat catctgctgt
    16621 gctgtattcc cgggaacgaa ccaatggatc aaatgatctc gccagtcttc tagccattgg
    16681 tccatcagca agtattgctt gtctaattga tcaaataagc tatataaact tgctaaatcg
    16741 atccgcatct ggttggtcca actttcttgg ataatctcaa aagtcaagcg catctgtgat
    16801 tgtagttgtt ggatctcttg ataaaaaagt tgcagatgat ccattgctgt tccagattcc
    16861 agtggcagct gatccaacat ttcttttgtc atcatgactt cttctggata ttgtccgtta
    16921 ggttgcttgc tgcttactaa gttcattaat ccctctgcca cgcaaatctg cgcttcgcat
    16981 agtgcagaga gttcttctcg ataaatcgac aaccagcgca aggcttcatg ttccttcggc
    17041 aaataacttt gtatgcggtc aaataactga ttcggctctt ccagttgctg aatttgttta
    17101 tggaagtagt taatacttag tcgtttgtca tttacttttt cagcaatttc cggcaaatga
    17161 tgcacttcat caatcaacaa ataaggacta gctggaagca atggttcttt tcgctgtgtt
    17221 tcttgtacta aaaatgcatg gttgactatc aaaaaattgc tttgtttgat tttcttttgc
    17281 aagaaacgca caaagtcttg tttgtaaaat gactgattcc ttgctaaaaa cccagtgcct
    17341 ctgtgcgcga catctgcaaa gaatgcatgg ttcagattgg ttaaatgtaa ttcatccaaa
    17401 tcaccggtct tcgtttgtgt tagccaaact aagacagcca tttggtacaa agcatattgt
    17461 ttttgtcccg gcggttccac aagtgtacct ttaaaacgct gtaaatcaat atagtggcga
    17521 tagcttttga taatcacagg ttgtatcggc tgatctagca attgattcag taatggaatg
    17581 tctttttcca tgatctgctg ctgcaaaaca agcgaaacgg tgctgatgac agctggtttt
    17641 tcaggtgtag ccaaataact caaaggcaac agataaccaa tcgttttgcc cattcctgta
    17701 gctgcttcga tcatcaaatc ttttgattca tcttttgtga aatggtcgta gacagcattc
    17761 atcagcctgt tctgttcttt tcgatacatc agtgttttac caaacatttt ttcttttgct
    17821 tgtttttttc ttggatatgt ccgctcgcca taatggacgg atgtgaatag ctccacttct
    17881 tttttctgca aagctagccc atcgatgatt tctaatgaga gatcaagggg ctccgggttt
    17941 tctttcattt cttcaagcac gtggtaaatg aaacgactcg tatccattcc tgtatggcta
    18001 cttaatcgtg caatctgctc taatgtgatg atcggcaatt tcctcattcg ttcttcgatc
    18061 agtaaaagaa gctgacctgt cacttcggca tcactatccg cctgatgagg attctcatga
    18121 ataaagccaa ggctttctga caaatcagct aagcgaaaac ttggttccgt cggcaagaaa
    18181 atttgtgcaa gttctacggt atcgatgccc ggtatcttca aactaggaag tccgcatcgc
    18241 atgagttcat ggttcaaaaa attataatca aaataaatat tatgcgcgac aaatatggtg
    18301 tctgccaaaa gattgtaaat cgtttgggcg acatcttcaa aataaggcgc tttttggacg
    18361 cgttgattcg tgatccctgt taaatgctgg atttgttttg aaatcttccg ccctggatga
    18421 atatcgatcg aaaaacgtga agtgatccga ccagcttcaa tcatgacgca gccaaattgg
    18481 atgatccgat cttcttttgg attcgtccca gtcgtctcga tatcaacgac tgcataagtt
    18541 cttggtttca aaatatcttc ctcttctcct gcctctaatg ttcttttgaa attatactac
    18601 aaagtgcaga aaaatactct catcgctttt taaggaaaaa aagacaaaaa agcccagaca
    18661 cataaatgtc tggactttta tttttaatat aggtaaatta agctttagta acgtttgttg
    18721 cttgaggtcc gcgttgacct tcttcaacgt cgaaagttac tgcttggcct tcttctaaag
    18781 ttttgaatcc gtcgccttgg atagctgaga aatgaacgaa tacatcgttt ccgttttctg
    18841 cagtgatgaa tccaaatcct ttttctgagt taaaccattt tactgtacct gtttccataa
    18901 taaaaatcct ccttgtgtat caacacatat tttgcaatta cttgaatggt gtatatctgg
    18961 aagatgttta tttgaaacaa gctaccacag attaccgaac aaaaactaca ttcttactat
    19021 atcataccgt ttacaaaatt gccagagttt ttccttattt ttttgtattt attctcttat
    19081 ttagaagata tatttgcaaa taaacgaacg ttttctcgaa atcaccccta taagattcag
    19141 aaaatttcta tttgctattt tcacttttgc aaaaaatttc aatttattat ctgaaaaagg
    19201 atgaa
//
LOCUS       DAYYAJ010000047        19146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000047 DAYYAJ010000000
VERSION     DAYYAJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.047"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..674
                     /locus_tag="ACHATZ_001401"
     CDS             120..674
                     /locus_tag="ACHATZ_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8403940.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(847..1284)
                     /locus_tag="ACHATZ_001402"
     CDS             complement(847..1284)
                     /locus_tag="ACHATZ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8403941.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1332..2279
                     /locus_tag="ACHATZ_001403"
                     /pseudo
     CDS             1332..2279
                     /locus_tag="ACHATZ_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2550..2985
                     /locus_tag="ACHATZ_001404"
                     /pseudo
     CDS             2550..2985
                     /locus_tag="ACHATZ_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3001..3834
                     /locus_tag="ACHATZ_001405"
     CDS             3001..3834
                     /locus_tag="ACHATZ_001405"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8403942.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4079..5104
                     /locus_tag="ACHATZ_001406"
     CDS             4079..5104
                     /locus_tag="ACHATZ_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8403943.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5242..5367
                     /locus_tag="ACHATZ_001407"
     CDS             5242..5367
                     /locus_tag="ACHATZ_001407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403944.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5392..6507
                     /locus_tag="ACHATZ_001408"
                     /pseudo
     CDS             5392..6507
                     /locus_tag="ACHATZ_001408"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6590..7486
                     /locus_tag="ACHATZ_001409"
                     /pseudo
     CDS             6590..7486
                     /locus_tag="ACHATZ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7502..8326
                     /locus_tag="ACHATZ_001410"
     CDS             7502..8326
                     /locus_tag="ACHATZ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8403945.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8358..9668
                     /locus_tag="ACHATZ_001411"
     CDS             8358..9668
                     /locus_tag="ACHATZ_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8403946.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9816..11075
                     /locus_tag="ACHATZ_001412"
     CDS             9816..11075
                     /locus_tag="ACHATZ_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8403947.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11085..11957
                     /locus_tag="ACHATZ_001413"
     CDS             11085..11957
                     /locus_tag="ACHATZ_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8403948.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11969..12799
                     /locus_tag="ACHATZ_001414"
     CDS             11969..12799
                     /locus_tag="ACHATZ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8403949.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12839..15022
                     /locus_tag="ACHATZ_001415"
     CDS             12839..15022
                     /locus_tag="ACHATZ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8403950.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15083..16303
                     /locus_tag="ACHATZ_001416"
     CDS             15083..16303
                     /locus_tag="ACHATZ_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8403951.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16300..17760
                     /gene="gtfA"
                     /locus_tag="ACHATZ_001417"
     CDS             16300..17760
                     /gene="gtfA"
                     /locus_tag="ACHATZ_001417"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8403952.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17837..18943
                     /locus_tag="ACHATZ_001418"
                     /pseudo
     CDS             17837..18943
                     /locus_tag="ACHATZ_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6502 a         2916 c         3724 g         6004 t
ORIGIN      
        1 tcataaacta aatcccttga ttttattgac tttcaaaagt ttatatatat aagttaatga
       61 ttatggtaaa atatatacat aatcattaac tcagaatatt tttaagaagg gaaacaatag
      121 tgagctataa tgtacaaccg ttaaggacaa atgaagagat acaagatttt cagttttggc
      181 taagaagaac aagtaatccg gaaagagata gttttttatt tttgtttgga atcaataatg
      241 ggttacgtat gtcagatatt ataggattaa aagttggaga tatacgaggc aaaagtaagc
      301 ctattattgt tgaacataaa acaggtaagc gaaaaccgat ttttatagat aatttgagag
      361 aagaaatttt attatatact gaaggtaaag aagaaaatga ttggttattt ccgagtcggc
      421 aacaaggtcg acatataaca agggatcgtg tctatcagat atatgcagat atagcagaaa
      481 aattaggacg agatgatata ggaacacata cattaagaaa aacttttggg tatcactact
      541 acaagaaaac tagagacatt gctaccttaa tgtttatttt taatcatagc tcgcaagcaa
      601 ttacaaaaag gtacattggt ataactgaag atgaaatagg agcatcactt cgtggattta
      661 aactaggtgt ataatttaaa aatttttata aacagtaaag acaaagatta aaagctattt
      721 ttatctagaa cgattttatt tcataaagtt cgcggaataa aagctggggt tactggtact
      781 tggtataggt tcgtgttata taaactaagt aattaaataa catttttagg ttggaaaagg
      841 atgaaattaa aaaaagagaa tcaatacggt ctcaaaaacg caaacgctgc ggaataagaa
      901 aaatactgat aactcaacgt ttagcagact atctaatagg gatagtctgc ttttttcgtt
      961 tttgaccata cttttgatct tctgcaaact tccttgcttt tcctctgtag aactcatgaa
     1021 atctgcaaaa cgaattgccg tttcctctgt ctgctttttc gcgatatggg tgtatatatc
     1081 atccatagtg gttttaatat ctgtatgtcc aagtcgaaca tacacttctt tgacagacgc
     1141 accagattca aagagcagac tacaatgggt atgacgaaaa ccatgaagtg tgatatgttt
     1201 taagttattc tctttaatga tgattttcaa attgtgattt acatagtcta aataaagggg
     1261 cttattttct tctgatgaaa acaaaagttg ttcaccatct tttataggtt tgattctcat
     1321 ttttagatat tatgcccgca cgtattcatc acatttctat tgtgaatcgc tttattcgac
     1381 cgacatttga cttttatcat aatatactcg gtttaaaatt actaatgaaa acaattaatc
     1441 aagacgatca taccatgtat cacctgtttt tttcagataa tcaccaccgt gtgggaactg
     1501 aactgacttt ttttgaagta caagaaggga acaaccagtt ttttggaacc aatacgattg
     1561 aacgaacgat actaaaagtt ccttccgaag catcattgca tttttgggaa atatattttg
     1621 aaacacaagg cgtttgccat tatggaatcg aatcattttc tggacgacct atcttacgtt
     1681 ttgaagggcc agatgcaaca cgattagctt tagttccctt acgcgaattt gaagatattg
     1741 atgattattt cccgtatgtc acagaccaga tccctacaga gcatgccatt ttaggaattg
     1801 atgccattca actacgcgta caatattccg atgcgactga acgagagtta ttgtcggtag
     1861 gctggtagca caaagaatac gtcagtttct tttcgggtca tcaacgagtg acaatcttag
     1921 aaaatcaaga cactcagttc tatcaagaag ttcagattat tcaagatcgt gagaatccag
     1981 ttgccattga agggattggt agcgtccatc atgttgcgtt tggcgtagaa aataaatctg
     2041 atttacaacg catcgacaaa caattacaag agcgcaattt tataaattca ggaataaaag
     2101 atcgtgaatt ttttgtatca ctttattatc gtgacccaaa tcaattattg attgaaattg
     2161 ctacagggga aggaaaccta gatgcaaaag cgtatgaaaa tcaaagtcca cgctttgaag
     2221 aaattccttt atttttaccg cagtacttaa atttcattcg tgaacaagtg gagcaataat
     2281 tagctaatca aatccatgac gaagagtaag aggttgactc ataagtcaaa aaataaagaa
     2341 aaacgcatca aatccattct aaagaatgtt gatttgatgc ggttcctatt tttataaact
     2401 atcattttcg actaataaaa ctacttatga gtcagcctct ctttttttct tgatttttat
     2461 acctctttct aggtaaacta aacatctgcc aaaaataccg tgtctcattg aaacattctc
     2521 aactatttca aaataaaggg gagtaccaaa tgaacgagta tattgaaatt atccacgcaa
     2581 gagaaacaac ttaaaaatgt cagtctcaaa attcccaaaa ataaaattag tatttttacc
     2641 agcgtatctg gttcaggaaa atcatcgatt gtttttgata ctattaccca agaagctggt
     2701 cgtcaactga atgacaccta tgatagtttt acccgtttat ttttacctaa atacagtcgt
     2761 ccagaggttg atgagattga taatctttct acggcgattg tggtcaatca aaagaagatt
     2821 ggtggcagtg cgcgctcaac gttagggacg attactaaca tcaatgcgtt atttcgtgtg
     2881 ttgttttcat gttttgcaac acctagttta ggatatgcaa atgcttattc atttaatgat
     2941 cctagtggaa tgtgttctgt ctgtcaggga atcggtaaga cgattgcatt tttaatagga
     3001 ttgctttatt tttcggctat tgataatgct gattttgtat tttctgatcc atttaccgca
     3061 gaagttttgt ctggaattga ggaacaggca aagaatcatg gttattttgt attggtgcat
     3121 aatgttaatt cagcagaaga tgttaaaact attcagagaa attggcgttt tgaggggttc
     3181 attgttgttg gtgtacgtgt aaaagaattt ttggaattaa ataacgtttt tgatggtcct
     3241 gttgtttata ttgatacgca tctttcaaaa gagacattaa gagaacttga acaacaaatg
     3301 aatcgtttat ttgtaaatac aaatgattac caagctagta cattggcagt ggaacatttg
     3361 attcattatg gtcatgagaa aatagcgttt ctttcttttg actacattgt aggtgaacca
     3421 agtgttattc aagaacgaca tcaagcttat atcgatacat taaagaaata tcatttaacc
     3481 tttaaatcgc attggatata taacaactct gaatttgagc gaatttatca agaattagct
     3541 gaatttactg caattgttgt tactggagat tatttagcgg ctaaattagt aaaattttta
     3601 aaaaataaga ataaacgagt gatggaacaa ttatcgatta ttggatttga tgatattact
     3661 tttgcagaat taatggatcc tgcgttgaca acaatacgac taaatccagg taacaagggg
     3721 gcgattgctt ttcaccaact gattggactg gccaaacaag aagaaataaa aaatcaagta
     3781 ctactattag agggagaatt gatttctcga gactctgttc acaaaatcaa ataaaaaagc
     3841 tctgtgaaat aagtatagtt aacaataaaa tatccgaacg atagagagaa tgaactgtga
     3901 tctagcagtt cctcgtcgca aattcactag aattagcatt atttactcta aattgttcgg
     3961 atattttttc ttgtttttaa ttatgattac agtgcctatt tttaacgaaa tttgcttatt
     4021 ttcaaaatgt atgattatat gttagtaaac actttcaaat atgaaaagag gagctattat
     4081 ggtaggaatt agggatgttg caaaacgggc gggtgtgtcc attgcaacag tatcgcgcgt
     4141 gctaaatgag gatccgacac tttcagtaac cgatgaaacc aaacgaaaga ttgcagaatc
     4201 ggttaatttt tatcattatg tcaaaaaaag tcccgttgca aaaaatttaa caaatattgc
     4261 attaataacg acagtttctg aagtagatga gttggaagat ccctattttc gaacgattcg
     4321 cagagggatt caagtcgaag cagaacaaag caaagtcaat ttgaagaaaa ttattcgttt
     4381 gtcagaaaca ccgttggagc aagacgattt gctaaaatgt caaggtgtgt tgattattgg
     4441 gcaggtgcta tcttctgtga ttgaagaagt atcagcaatt aattcgaatg tcgtagtgat
     4501 tgatgaccct agtattgaag ctgaagtaga tgcagtttat actgatttaa caaaagcaac
     4561 gaatcagcat ttagatcgat tgtataaaaa aggacaccga aacattgcgt ttatcggagg
     4621 aggacgtgtt cgtttagata gccaaggaaa taaacacgtg tcagatgatg attatcgaaa
     4681 aatagcctat gagacgtgga tgaaacaaaa aggattagaa aaatatagtc aaatttattt
     4741 agaaggctgg accacacttg acggaatgca ggcagcagat ttacttttga aaaaacatgc
     4801 taaagataag ttgccgactg ctatcatggt aggaaatgat cctattgctg tgggggttta
     4861 tcgttcactt caaaaaaatg gtctacatat tccagaagat atttcgattg tgagttttga
     4921 taatattgaa gttgctgaat ttttaacacc tgcattaaat acggtgaatg tgaatacaga
     4981 agaaattgga aaactagcag ttcgcatggt taatgaacga ataaaagaag ttagaaatgt
     5041 ttcgatcaaa gtgattgttt ctaataatat tattgttaga gaaagtgaaa aaaatatcga
     5101 ttaaaaaagt aaacatccaa aaaagggtgt ttactttttt gtttactaat atttttagta
     5161 aaaaatctcg acaaaacatt aagtaaacag ttatggtgtt gttaacaaaa agaaaacgca
     5221 ttcaaaacag gaggaattaa gatgaaacat ttcaaaaaaa tagctttaac agggatagct
     5281 gttttaactg ggacattact cgcagcatgt ggtgggggag atagtaattc taaagacggg
     5341 caagtaactt taagctatgc gagttgagat actgaccaag ctccaggact aagaaaaatg
     5401 ttagatgaat ttgaaaaaca aaatcctgat atcaaagtag aaatggagac aacgccttgg
     5461 gatcaatatt gggttaaatt agaagctgca acaacgggag gtaatatgcc agatgtagta
     5521 accatgcact ctagcgaatc ctataaatat atgtcacaga atgctttgat gggacttgat
     5581 gatgttatca aagaaaatga tattgacatg ggaaacttta ctgaaggaat tgctgatttc
     5641 tatacgcttg aagacgaact atacgcaatc cctaaagatg cttctgtagt ggggctatgg
     5701 tacaacaaag aattatttga taatgcaggt gtcgattatc cagatgaaac atggacatgg
     5761 gaaacactca gacaagctgc aattgattta acagatgaat caaaaggcat ctatggtttt
     5821 gcatcacata ttggaacaga gactggctac tggccattta tttatcaaaa tgggggagaa
     5881 gtatttgccg atgacaatac aaaatcaggt ttgaataccc cagaagcaca agaagcttta
     5941 gctttttatc gagatattgc acttgttgat catgcttcac caacaattac agaattacaa
     6001 gatcctgata agttaagtcg ttttcaatca ggtagattag caatgatgat tgaaggaaac
     6061 tggtttgcac cgggattttt gaaaaatgac tatctagttg aaaatgttgc ttctgccgtg
     6121 ttacctaagg gaaaagttaa tgcaacaatt acaaatggac ttggttgggc tgccagtgcg
     6181 agtacaaaat acccagaaga agtgaaaaaa ctaatggcat ttttagctag tgaggacgcc
     6241 aataaaatcc aagcggaaac gggggcatca atcccagcac taaaaggctt tgaagacgag
     6301 tgggctgctt cttcagatac aattgatttg agtgcttttg gaaaaatgtt ggagtatgga
     6361 gtccctcgtc catacaataa agatggatta cgagctgaac aagcagaagt tcaaattatt
     6421 aaccgtattc tgtctggtga agtaactgtt gaagatggaa ccgctcaaat tgaaaaagaa
     6481 gtcaatgcga tattgagcgg aaattaaatt agttaattat acaatataca ggcgaaagga
     6541 ttctatttcg tctgtatatt aatggatgaa taccaaaaag ggggatatta tgaaaaatcg
     6601 aatttttgga aaatatcgta aagatcaagt gatatggggc tatattttta tttttccaac
     6661 ggcattagga ttaattttat taaatatatg gccagcaatt caaacagcaa ttttaagttt
     6721 tcaaaaaacg gtagggttcg gtgatactga atgggtcgga ttaagaaatt accaaaaaat
     6781 gtttgcagac ggtgaagttt ttcagtcttt attaaacaca ttaatttatg cagtggtttc
     6841 gatacctagt attgttgtat tatccttgtt ggtggcagtt ttaatgaata gaaaagttaa
     6901 aggattgtcg atttatcgga caatttattt tctaccagtc gttgctgcac catccgcagt
     6961 cgcaatgatt tggcgctgga tgttcaataa tgattatgga ctgattaata acatgttggc
     7021 taaaataggt ctcgatggac cagcatggtt aacggatcca tcgattgcga tttattctat
     7081 tatcattgta ggagtttgga gtgctattgg ttataacatg gtgttattac tagccggctt
     7141 ataagaaatt cctaaagatt attatgaagc agcggaaatt gatggtgcta gtccaatcca
     7201 gcagtttttc aacattacgt taccattagt tacaccaaca ttattttttg tagttgtaac
     7261 aacagttatc aatgctttcc aagtatttga tgttattttt atgatgatta ctcctaatag
     7321 taccgccatg tttaaatcac aatcattagc ctatttattc tataaacatt catttatttt
     7381 aaatgacaag ggatatggtt cggcaatcgt tatgttcctt ttagtgatta tattaattat
     7441 tactgcaatt caaatgaagt tgcagaaaaa gtgggtaaac tattaaggag gctatcaaat
     7501 tatgaaaaat aaattttttt caaatgcgcc aatccatatc atgcttattt taggtggtat
     7561 tgcaatggca ttacctttta tttggatgat tttaacttca gggaaaacgt tggttgaatc
     7621 tacccaaatt ccacctacaa ttttccctga gaaatttcag ctaagtaact atcaacaagt
     7681 acttgattta ttaccattca tggcttttta tatcaataca tttttaatgg ttgttggacg
     7741 agtgattggc tcggttttat tttcagcaat ggctgcatat gcttgtgcgc gtctaagttt
     7801 tccagggaaa aatatccttt tcagcttagt acttattcaa atgatgattc ccggttccat
     7861 ttttattact ccacagtatt tattagcaca aaaattgggt attttaaata caattccagc
     7921 attaatatta ccaggggtag ttagtgcgtt tggtacgttt ttattaagac aatttttctt
     7981 aggtttacct gatgaattgg aggaagcggc ctatttagaa ggtgccaata tttggacgat
     8041 ctttttcaaa attatgttgc cgcttgctaa atcaggattg gttgcacttg gaatattcac
     8101 agcgttgttt gcatttaaag atttaatgtg gccattgatt gtcaatactt caattgataa
     8161 gatgccttta gctccgggat tagcaaactt acaaggacaa tatgccacga acttcccaca
     8221 attaatggca ggttcgttat tagcaatttg gccaatagtt gtgatattca tcgttttcca
     8281 aagaaaattc attgaaggaa ttgctacaac aggtagtaaa ttataaaact attaattagt
     8341 gattggagag taagtacatg acaaaaatta catttattgg tgcaggaagt actatttttg
     8401 caaaaaacgt attaggcgat gcgatgttaa ctccaagtct acaagatgca catattgcat
     8461 tgtacgatat tgatgaaaaa cgactaaaag aatcaaattt aatgcttaaa aatattaata
     8521 aaaattcgaa tgaaaatcgt gctgatattg tttcttatta tgatcgcaaa gaagcgttaa
     8581 gagaggctga ttttgttatc aatgctattc aagtgggtgg ttataaacct tcaacagtga
     8641 ttgattttga tattcctaaa aaatatgggt tgcgacaaac tattggtgac accatgggga
     8701 ttggtgggat ttttagaacc ctacggacac taccagtaat gttagacttt gctaaagaaa
     8761 tggaagaagt ttgtccaaat gcttggttat tgaactatac aaatccgatg gcgatgttaa
     8821 ccatgggtgt tttaaaagcg acgagtatta agactgttgg tttatgtcat agcgttcaag
     8881 tatgcgtacc agaattgttt gagcatttag gaatagccga tcaatatgac ttaaatgact
     8941 ttcaatggaa aattgcaggc attaatcata tggcattctt gttggaaatt acaaaaaatg
     9001 gggaagattt ctatccaaca attcgtcagt tagctgcaga aaaaccaaat ccacatccag
     9061 attctgtacg ttttgaattg ttgaaacgtt ttggttacta tattacagaa tcaagtgaac
     9121 acaatgcaga ataccatcca tactttatta aatcaaaata cccagaattg attgacgaat
     9181 tagggattcc gttggatgaa tacttacgtc gttgtgaaca acaaattaat gattgggaaa
     9241 gcatgcgaga ggacattgta aataatgggg agttaacaca tacgagaagc cgtgaatatg
     9301 cttcatatat catggattct attacgacag gaactccgtc agtgattgca ggaaatgtct
     9361 taaataaagg cttaatcaca aacttaccga cagatgtttg tgtggaagta ccttgtttag
     9421 tcgataaaaa tggcgtgcaa ccaacctatg tcggtgaatt gccaacacaa ttagctgctt
     9481 taaatcggac gaatatcaat gttcaagaac tgacagtaga agccgcttta acactagata
     9541 aatcaaaaat ttatcaagca gcgtatttag atccacatct atcatctgaa ttgtctttaa
     9601 cagatatcaa aaatttagtg gatgatttaa tcgaagcaca tggggactat ttacctgaat
     9661 ataattaaaa agaagctgaa agagtaagga attttcaatt ccttactctt ttcttagtta
     9721 aatttagtaa aaatattgac aaaacactta gtaaacagtt acattagtgt taacaaaaag
     9781 aaaacgcatt caaattgtat aaggaggagc ataaaatgaa gtggtctaaa aaaattattt
     9841 tagcttgtat agcactagga gcaacgatct ctttgactgc ttgtggaaaa aatcaggaag
     9901 atgcaagcgg taaggtagaa atagagtttt ttaatcaaaa aaaagaaatg actcaaacga
     9961 ttcaggagat tgctaaagat tttgaagcaa aaaaccctga tattcatgta aaagttgtgg
    10021 atgtaccgaa tgctggtgaa gtaattaaaa cacgtatgct tgcaggcgat gtaccagatg
    10081 tcattaatct ttatcctcaa agtatagaat tgaaagaatg ggcgaaagct ggttatttag
    10141 aggacttaac cgaagaaccg tatttagaga atatcaaaaa tggatatgca caacgttttg
    10201 ctattgagga tcgtgtatat agcataccat taaccgctaa tgtctatggt ttttactata
    10261 acaaaactgc ttttgaggaa atggggattc aagcaccaga aacttgggca gaatttgaaa
    10321 aaatagtagc tgagattaaa gatcaaaaca aagtaccgtt tgcgattgca ggagcagaag
    10381 gctggacttt aaatggttat caccaactag ctatagcgac tgctgctggt ggtgaagagg
    10441 aagcgaacaa tgtttggcgt ttctcagaag ttaatggaat taatgctgaa agcaaagaaa
    10501 tgcaaaaaga ttttaatcga ttagatttgt taagagaacc aaaagctttg caaaacaact
    10561 ggcaaggtgc cggttataac gatacagtag ttacttttac aaaaggtgat gcgttgatta
    10621 tgccgaatgg ttcatgggca atgccgatga ttcattcaca aaatccagat tttgaagtag
    10681 gaacttttcc ttttccagca gatgaagcag gtcaaagtct aacgattggc gcaggagacc
    10741 tagccttatc agtttctgca acttctcaac ataaagaaga agccaaacgt tttgtagaat
    10801 atatgacaac acctgaagcg atgcagaaat attatgatgt agatggtgca ccgtgtgctg
    10861 ttgagggcgt tatcgagaat actcaggatt caccattaag tggtttaact gaacttgctt
    10921 ttactgatcg tcatttagtt tggttagcca aagattggaa tagtgaaaat gatttttata
    10981 ccttaacaac aaattattta cacaatggca atcaaaaagc catgattaac gcattaaatg
    11041 ctttctttaa tccaatgaaa gctgacggaa ggtaggaaaa taaaatgaaa ataaaagaaa
    11101 ttttaaatcg ttactgggca atcatttttg tagcaattcc aattattttg cagatgattt
    11161 ttttctactt tccaatggtt caaggtgctt tttacagttt aactaattgg actggtctga
    11221 cttataattt tgattttgtg ggtttgaaca attataagat tcttttttct gatccgaagt
    11281 ttatcaaatc gattggcttc actttaattg tgacgctttg catgattgct ggacaaattg
    11341 gttttggtat tttgattgcg cgggcgttga acgcaaatat taaagggaaa acattcttta
    11401 gagcatggtt cttttttcca gcggtcttat cgggcgtggt tgtttcttta gtatttaaac
    11461 aaatttttaa ctatggttta ccaaaaattg gtagtatgtt aggcattgaa tttttacaaa
    11521 ccagtctttt aggaacaaca tggggagcga tttttgcagt aatttttgtt ttactttggc
    11581 aaggaattgc catgccagtc atcattttct tagcgggttt tcaaagtatt ccacaagaaa
    11641 ttattgaagc tgctagaatg gatggggcga atgataagca aattttttgg aatattgaaa
    11701 tcacctactt attaccaagt atttcaatgg tatttattat ggcattaaaa ggtggcttaa
    11761 cagcatttga tcaaattttt gctttaactg gtggtggacc aagcgatgcc acaacatctt
    11821 tggggttact tgtttataat tatgctttta aaaacaatca gtttggttat gcgaatgcta
    11881 ttgctttagt cttatttatc ttaattggga ttgtttcact ggcacaatta aaattatcga
    11941 aaaaatttga aatctaggag gtttcccaat gaaaacatca aaagaaaaaa atatttctaa
    12001 atacttgctt ttattattag ggggcttcat gattttagtt cccttagttt caacgctatt
    12061 cggttcattt aaaacaacac ccgatattat gaataatttc tttcgttttc ctaatccagc
    12121 gactttggaa aatttccgaa gattgttagc ggatggaatt ggtcattact tttggaattc
    12181 ggcaatcatc acaatcattg cagtagttgc tgtgacagca attattccta tggctgctta
    12241 ttcactagca agaaaaatga ctcgtgggaa agcatacgga ttgatgtatt tatttttaat
    12301 tttaggaatt tttgttcctt tccaagtaat tatgattccg attaccgtta tgatgagtaa
    12361 aattggttta gcaaatatgt ggggattaat cttactctat ctcacttatg ccgtaccaca
    12421 gacactattt ttatatgttg gttttattaa actaaatgtg cctatcagtc tggatgaggc
    12481 tgctgaaatt gatggggcta gtcactttac aacttatttc aaaattgttt tcccaatgtt
    12541 aaaaccaatg catgcgacga ctattattct taatgctcta tggttttgga atgatttcat
    12601 gctaccatta ttaattttaa atcgtgattc taatatgtgg acactaccat tgtttcaata
    12661 taattatacg ggacaatatt ttaacgacta tggtccaagt tttgcttcct atacagtagg
    12721 aattgtcacg attacactag tttatcttgc tttccaaaga agtattatag ctggaatgag
    12781 caatggagca gtgaaataac caataaatta ttctcattat aaaggagttc ataaacttat
    12841 gattatattt gacaatcaca cacaaacatt tcatttacaa aataaaaaaa ttagctatct
    12901 attaggaatc gaagaaaata attatttaac gcatctttat tttggaaaaa agattacgca
    12961 ctattctggt gggtatcgct acccccgaac agatcgttcc ttctcaccca acaatgcaaa
    13021 tgcgcaagat cgtttattat ctttagacac attaccatta gaatttccag gatatggaca
    13081 tggtgatttt agagaaccgg cgcataatat taaattagga aacggtagtc gtgtaaatga
    13141 ttttcgttat gatagctacg aaattcaaaa aggaaagccc agcttagcgg gattgccagc
    13201 cttatatacg gaagatgatg atgaagctga aacgctgatt attcaattga aagacgcgat
    13261 tagtggattg actttacagt tgttctacac aatttatcaa aatcacagtg taattattcg
    13321 ttctacgcaa ttagtaaata atagtgatga aacagtggag attaaccgct tagctagtca
    13381 atcaattgat tttccaaatc gtccactcga gttaattcat ttaaatggta cctgggcaag
    13441 agaacgtcaa atgactagag aaaaaattca tacagggata aaagtattag atagtaaacg
    13501 cggatcaagc agccatcaac aaaatccgtt tgttgcttta gtagatgcca aaacagatga
    13561 atttcaagga gaggcatatg gatttaattt agtttatagc ggtaatcacg aaacggtgat
    13621 tcaaaaagat ccatttgacc aaacacgggt cattatggga attaattcat ttaattttgg
    13681 ttggcaacta aaaactggcg actcatttca aacgccagaa gtcgtgatgg tttatagtaa
    13741 tgaaggtctg aatacgttat cacagacgta tcatgaattg tataatcacc gtttaattcg
    13801 tggaaactat cgtttaaaag aacgtcccac gttaatcaac aactgggaag caacgtattt
    13861 tgattttaac gaagaaaaaa tcatgaatat tgtcgatgat gcgaagaact tgggcattga
    13921 aatgtttgtt ttagatgatg gttggtttgg cggtcgtgaa aatgacttca cttcattagg
    13981 tgattgggtt gaaacagaag gcaaacttgc ttgtggtttg gaaaatttag ccaagcaagt
    14041 gcatgataac gggatgaaat ttgggatttg gtttgaaccg gaaatgattt ctgaagatag
    14101 tgaactattt cgagcacatc cagattgggc attgggtatt ccaggtcgtg accgctcaat
    14161 tagtcgaaat caatatgttc ttgatttttc aagacaagat gttcgtgata atatctatca
    14221 acaaatgacg gcgatttttg atcgtgtacc gattgattat gtgaagtggg atatgaatcg
    14281 aaatatgaca gaagtctatt ctgtcttgtt ggactcaacg atgcaaggtg aagtggctca
    14341 tcgttatatg ttagggttat atgaattttt agaaaagtta acaacgaatt atccaactat
    14401 tttatttgaa agttgttcgg gtggaggcgg tcgttttgat gcaggtttct tgtattacat
    14461 gccacaaact tggacgagcg acaataccga tgccgtggct cgtctgaaaa ttcaatatgg
    14521 aaccagctta gtgtatccaa tttcaagtat gggtgcgcat gtttcagcag tcccaaatca
    14581 tcaaacagga agagaaacga gtttaactat tcgtggcaat gctgcgatgg ctggtgtttt
    14641 tggttatgag ctagatctaa cgcaattaac ttctgaagaa aaagcagaaa ttaaagaaca
    14701 agttgccttt tataaaaagc atcgccaact tttgcaatat ggaacttttt atcgcttgga
    14761 aagtccgttt gaaggcaatg atactgcatg gatgtttgtc tcaaaagacc aaacagaagc
    14821 catggtcttt tatttccgag tattagcaga agctgcgcat ccattagtca cattgaaatt
    14881 agcaggatta gcagaagaag caacttatca aatggaagat gtaacatttg ctggcgatga
    14941 attgatgaat ctcggttttt atattgactc agaactacat ggtgattatg cgacacaacg
    15001 cttttatttg aaacagatat agtagcttga atttacaata taagtgcaac accaaaagag
    15061 actcattcca aacgaagaaa atatgtttta tttacacgca tttactaaaa agcagcctga
    15121 tttgaattgg gaaaatgaag aagtacgtga agaaatctat gcaatgatta actattggtt
    15181 agataaagga ctaggcggat ttagaatcga tgcgatatta aacatcaaaa aaaaactaga
    15241 gtatggcatc tttgaatctg atggagaaga tgggttagcc ttcattgggc cctggattct
    15301 taatcagtcg ggcattgaag tgtggttaaa agaaatgaga gagcggacat ttaaaccaca
    15361 taatagtatg acggtagcag aagccgatgt tcctaatgaa cgtttagatg aatatattgg
    15421 tgaagatggc tattattcga tggtttttga ttttagttat acggatattg atgtacctga
    15481 aactggtgaa tggtttaaag atagccaatg gacatggacg gatatgcgta ccaatatttt
    15541 tacgaatcaa ttagttacgc aagataaagg ttggggcgcc ttatatttag aaaaccacga
    15601 tcaaccacgt tcaattaata aatatattcc agaagaatgg atcaatgatt atagtaaaaa
    15661 aatgttggcg acgcttttta tgttactaag aggtacccct tttatttatc aaggacaaga
    15721 actaggaatg accaacatag aaatggcatc attagaagat tttgatgatg tagctacgca
    15781 ttctcagtat aaacgggcat tagaatatgg attggcacca gagcaagcat tgaaagctgt
    15841 caataaacga agtcgggata attctcgcac acctatgcaa tggaatacgc aaaaaaatgc
    15901 cagattctca acttctgata atgtatggtt aaaagtaaat cctaattatc cagaactcaa
    15961 tgcagaagct cagttaacca atcaacagtc ggtattcaat tattatcatc aattaattaa
    16021 attaagaaaa aatagtgtat acaaagacgt gctaatctac ggacaatttc taccagtgaa
    16081 agataaaaat gaacatattt tgctttatga acgccaatta gaagataaaa tcgtattagt
    16141 tatcttaaac gttacagcaa cagaacaaga atattatgta gatgaacagt accaacaagt
    16201 attaatcaat aattatgaag atgttttatt agatcaaagt cgaaaattgc gtttacgacc
    16261 gtttgaaagt attgtattag ctaattatgg aggacaatta tgaaaattaa aaacgaagca
    16321 atgctcatca cctatgcaga tagtttagga aaaaacatgg gcgaattaaa acaagtctta
    16381 gacaaacatt tacaaggtgt tattggtggt gtacatctgt tgcctttttt tccatcgaca
    16441 ggtgatcgag gctttgcgcc gagtgattat acgacagttg atccagctct tggaacatgg
    16501 gaagatgtgg aagcattagg tgaagattat tatttgatgt ttgattttat gattaatcac
    16561 atttctcgtg aatcaaaatt tttccaagat ttcaaaaaaa accatgagca gtcaccatac
    16621 aaagaaatgt ttatccgtat tcatgaattt ttcccagaaa atcgaccaac acaagaagat
    16681 attgatttaa tctacaagcg taaagacaaa gcgccgtttc aaacggtaga atttgcggat
    16741 ggtacgacag aagaggtttg gaataccttt ggggaagaac aaattgattt agatgtgacc
    16801 aaagaagttg tgaaagaatt tattcgagaa accatcaaag atatggcgaa ccatggctgt
    16861 tctttaattc gattagatgc gtttgcttat gcgattaaaa aattagatac caatgatttc
    16921 ttcgtggaac cagatatttg ggaactttta gacgaagtgc gtcaagaagc agcgaaatat
    16981 gacgtcgaat tattaccaga aatccatgag cattattcca ttcaaatgaa aattgccaat
    17041 catgattatt atatctatga cttcgccttg ccaatggtca ctctgtattc actttatagt
    17101 ggaaaatcag aacgtttagc caattggttg aaaatgagtc cgatgaagca gtttacaaca
    17161 ttggatacgc atgatggaat tggtgttgtg gacgctcgtg atttattaac agatgaagaa
    17221 ttggattaca cctctgagga attgtataaa gtcggggcga atgtgaagaa ggtctattca
    17281 agtgccaatt acaataattt agatatttat caaattaaca gcacctatta tagtgcatta
    17341 ggcgataatg accgtagcta tctgttagca cgtgcgattc aagtatttgc gccagggatt
    17401 ccacaaattt attacgttgg gttattagca ggaaaaaatg atattgaact gttggaagca
    17461 acgaaagaag ggcgtaatat taatcgtcat tactatgatt tagccgaaat tgaagaagaa
    17521 gtgcaacggc cagtgattca acaattattt gcacttttaa aatttagaaa tgaatctgcg
    17581 gcgtttgatt tggatggaac gattgaagta gcaacgccat cagaatcaga aatagtgatt
    17641 acacgtaaaa ataaaaacgg tagtgatagt gcaacattaa cggctaactt ggatacgaaa
    17701 gaatttatca ttgagtctaa agatataaaa attttcacta gtgttgcatt aaaattttaa
    17761 cctattccat agaacaaaaa atcctttata ctagcacttg gatgacttat ccaagaccaa
    17821 taaaaaggac tcatacatgg ataagtataa aacaaataca gcatttaata aatggttttc
    17881 tgccataaaa ttaggaaaac tttctgtcaa ctcccaaaaa tgtatttttg attacaattc
    17941 gtatcgtaaa aaactgtctt ttgaagctgt gctcaagctc tttctctacg cagttaatga
    18001 agaaaaagaa agtttgcgtg atttgagtac agctcttatc gaccgatcgc tccaactaga
    18061 aaccgatgtg tcatcgatta gtcataccca attgtctcga gcatttaatg cacttgaacc
    18121 taaagtgtta gaagaaattt ttcaacaact gttagaaaaa gttcaacatc aaatcaaacc
    18181 aaccaagcga aatcatctgt atctgattga ttccagtacg ttttcgctta gtttaaaacg
    18241 tcataagtgg gcgactttcc gtcaaacaaa atcaggtgtc aagttacact taaactattg
    18301 ttatatggac gatactacga tgtatccaac tgattttacc ttaacaaatg cccatgaaca
    18361 tgatctcaat caactaccag tattggtgaa tcaaccagag gcaacttacg tgtttgatcg
    18421 tgggtattag gactttgaaa agatggatca gatgcattgg gatggctatt tcttcgtcac
    18481 tcggattaaa aaaaacacca aagttcacgt gatagatact ctggaaacat cgccagaaac
    18541 agaaattcta cgagacgagc tggttcgttt aggatctaaa acctatctaa ccgccaattt
    18601 ccgactagtc actgttcaag ataagaacgg acgtgtgttt caatttatca ccaatcgaat
    18661 ggacgtctct tcaaaagaaa tctctgatat gtatcacgct cgttggcaaa tcgaattgtt
    18721 tttcaagcac atcaaacaac acatgaccat taaaaccttc ttttctcaaa gtgaaaaagg
    18781 cgtgcagaat caattgattt taacgatgat ttccgctctc ctcacgtttc tcatcaaatt
    18841 ggagacgaaa acggaaaaat cagtgttcca aatcaaacga ttctttcgtt atttactgtt
    18901 tcagcccttt gaatgttgtc tagaaaaatt aattcccaca tgagaaaaaa gaaatttggc
    18961 tcgttacttt tggttttcag ttgaataaca gataaaaaat tttctggaaa aagtcatcct
    19021 ctgcgtcagc acagtttttt ttgacttatt ttcagaggaa atagttttca gaatattcta
    19081 aaatgtttta tgcaacacta gtgatatttt ttagaattag atttctaatg tgcatttttt
    19141 gagtat
//
LOCUS       DAYYAJ010000048        19077 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000048 DAYYAJ010000000
VERSION     DAYYAJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.048"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..2402
                     /locus_tag="ACHATZ_001419"
     CDS             108..2402
                     /locus_tag="ACHATZ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8403953.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2395..3060
                     /gene="pgmB"
                     /locus_tag="ACHATZ_001420"
     CDS             2395..3060
                     /gene="pgmB"
                     /locus_tag="ACHATZ_001420"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8403954.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            3053..4060
                     /locus_tag="ACHATZ_001421"
     CDS             3053..4060
                     /locus_tag="ACHATZ_001421"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8403955.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            4082..5101
                     /locus_tag="ACHATZ_001422"
     CDS             4082..5101
                     /locus_tag="ACHATZ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8403956.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(5382..6749)
                     /gene="nhaC"
                     /locus_tag="ACHATZ_001423"
     CDS             complement(5382..6749)
                     /gene="nhaC"
                     /locus_tag="ACHATZ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8403957.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(6858..8279)
                     /gene="tyrP"
                     /locus_tag="ACHATZ_001424"
     CDS             complement(6858..8279)
                     /gene="tyrP"
                     /locus_tag="ACHATZ_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8403958.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(8522..10399)
                     /gene="tdc"
                     /locus_tag="ACHATZ_001425"
     CDS             complement(8522..10399)
                     /gene="tdc"
                     /locus_tag="ACHATZ_001425"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8403959.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(10690..11946)
                     /gene="tyrS"
                     /locus_tag="ACHATZ_001426"
     CDS             complement(10690..11946)
                     /gene="tyrS"
                     /locus_tag="ACHATZ_001426"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8403960.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(12058..12263)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12526..13827
                     /locus_tag="ACHATZ_001427"
     CDS             12526..13827
                     /locus_tag="ACHATZ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8403961.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(13896..14495)
                     /locus_tag="ACHATZ_001428"
     CDS             complement(13896..14495)
                     /locus_tag="ACHATZ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8403962.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            14749..15630
                     /locus_tag="ACHATZ_001429"
     CDS             14749..15630
                     /locus_tag="ACHATZ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8403963.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            15643..17253
                     /locus_tag="ACHATZ_001430"
     CDS             15643..17253
                     /locus_tag="ACHATZ_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8403964.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(17330..17989)
                     /locus_tag="ACHATZ_001431"
     CDS             complement(17330..17989)
                     /locus_tag="ACHATZ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8403965.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(18272..18937)
                     /locus_tag="ACHATZ_001432"
     CDS             complement(18272..18937)
                     /locus_tag="ACHATZ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8403966.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         5750 a         3871 c         3591 g         5865 t
ORIGIN      
        1 ttattttttt aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg
       61 gttgcgttta ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca
      121 aacgactatt tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc
      181 ttcgactgca agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact
      241 ttgaagaaac ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc
      301 ccgataagac acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga
      361 tcaacgcctt gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg
      421 cttctttgga accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc
      481 gtacattcac aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga
      541 gcattgtcaa aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg
      601 gaacagtcaa actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg
      661 atgaacattt ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga
      721 ctaaaactat tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc
      781 atttcgttaa tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg
      841 gttgcttgtc tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg
      901 ttcttactag tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa
      961 cgaaccagac aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac
     1021 gatggaagct tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat
     1081 tcaatttgtt ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc
     1141 ccaaaggatt cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg
     1201 ctgtccctct ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc
     1261 ggcataatca actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc
     1321 tttatcctat ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg
     1381 aggagattca ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg
     1441 gcgatacgga atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct
     1501 gggcagaccg tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg
     1561 gcccaaacga atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt
     1621 gggtcttgcg ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta
     1681 tcagcgaaga agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg
     1741 acgaagaatt aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg
     1801 tatccgatat tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc
     1861 ttagatcttg cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata
     1921 cttttacaaa agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg
     1981 agtcttccct ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca
     2041 aagctgtaga aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca
     2101 cagaagacgg cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat
     2161 ttgctcaaat gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt
     2221 ggaaagctta ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag
     2281 atgaacaagt tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac
     2341 agtcttattc tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt
     2401 aaaggggtat tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt
     2461 gcttggcgaa agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag
     2521 ctaaaaggcg tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa
     2581 cactcttttt caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta
     2641 gagatgatcc agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat
     2701 tctttaaaag aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc
     2761 ctacttgaaa aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca
     2821 acaaacggaa aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa
     2881 gcagaagaat gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt
     2941 ggcgctcaac caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc
     3001 cctgatacga cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa
     3061 tccagagatc tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa
     3121 cgactactta gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc
     3181 agacaaagaa ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct
     3241 aacagacaaa agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc
     3301 ttcttggggt gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac
     3361 gaaaggctgg tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg
     3421 tgtggtcttt tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat
     3481 cacttaccgt ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga
     3541 aaccatctgc aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga
     3601 catctatgaa catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa
     3661 actgcctact ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc
     3721 aacactgcaa gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca
     3781 tcatcctagg ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat
     3841 gactatagca acttctaata aaagtatggt attattttct acgactggat tcgaagctga
     3901 tttctctgtc aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga
     3961 aatccctgat atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga
     4021 acggatcagt cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat
     4081 catggctatt accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc
     4141 tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat
     4201 tatggatgaa ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc
     4261 cagtgccatc ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt
     4321 ttatcttgag atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc
     4381 aatcgctact gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca
     4441 aaaacaagta gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta
     4501 tttataccaa aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga
     4561 aatcgtttat gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat
     4621 ccagaatgga cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt
     4681 cgaacgatat ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag
     4741 tgtagtacta gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga
     4801 agccacagca ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca
     4861 aatgtatggc tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt
     4921 ctcaacatta acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg
     4981 tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt
     5041 ggtcatccct catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta
     5101 aatagctgac tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag
     5161 aagatactac ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga
     5221 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
     5281 aaataagctg aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct
     5341 tggagttaaa cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga
     5401 cgattcaata aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg
     5461 aatggcaagt aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact
     5521 ccccacggta cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag
     5581 gtttttgata agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg
     5641 gacagatact gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg
     5701 actaaacgag aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc
     5761 agatatttga tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct
     5821 aacatactca tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt
     5881 gcgacataac cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg
     5941 ataaaagaaa gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt
     6001 tttttgattg cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa
     6061 aagctcgctt tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg
     6121 ttatgtccta atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa
     6181 cgcataccag cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc
     6241 gtatctgata aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta
     6301 gtcattgcat cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc
     6361 gaaatcgttg taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct
     6421 gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg
     6481 gttccagata aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga
     6541 atgatccctg gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt
     6601 ttaaatctgc cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag
     6661 agctggaatc cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata
     6721 ctttctttta aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc
     6781 tgccatgaaa aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg
     6841 ctgaaagact tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa
     6901 tctttcttga agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct
     6961 actaataatg catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata
     7021 gagaagatca gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg
     7081 taaggacgtt cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa
     7141 atgtacacga tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt
     7201 acgtcatcca ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga
     7261 acgacatttc tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg
     7321 aatccttcac gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat
     7381 tgtaaaagca caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag
     7441 cctaggattt gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca
     7501 tctggtacga catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc
     7561 aaagcgataa atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa
     7621 acagaactca tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa
     7681 tcaatatgtt ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt
     7741 gagactagtc cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg
     7801 atatataccc ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag
     7861 ttcaagatcg tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta
     7921 ttgccaagtt ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc
     7981 attaccatac ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg
     8041 ctgcccagtg ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa
     8101 agctcaccgg aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa
     8161 aaaatcaatg tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt
     8221 gccattgtca gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc
     8281 gttgacactc tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa
     8341 taaacgaaac aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt
     8401 tcttcaaaga caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca
     8461 gatccaaaca tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg
     8521 cttattttgc ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa
     8581 gagctgcttt gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg
     8641 tcattacgct ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga
     8701 atcctagttg gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag
     8761 tatgtgaagt taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat
     8821 atacgtcatg gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata
     8881 catagtctac catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc
     8941 ctactttaaa tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg
     9001 cacccattaa tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa
     9061 cgctggctgc tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg
     9121 ggatatctgc gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc
     9181 ggatatcttt gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt
     9241 caatcgttac agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga
     9301 tataatcttt gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa
     9361 atggaatgaa gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag
     9421 catctacgtg taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga
     9481 ttttatcgat accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta
     9541 ggattggtgt tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca
     9601 tgcgatagtt gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt
     9661 cggcagcttt tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt
     9721 ctaaatgttt tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc
     9781 tgtctaacag attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc
     9841 cagcaactag ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg
     9901 cataccaaag tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat
     9961 ctttatagct cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg
    10021 caggagaaga ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta
    10081 gtaaagatgg catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc
    10141 aaggaacgga atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat
    10201 tcacagtgtg ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt
    10261 gaggcatgta gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt
    10321 tatagatttg gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt
    10381 tcgacaatga ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc
    10441 ctacattaca cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat
    10501 tttaatatgt atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg
    10561 gaatttacaa atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt
    10621 taggatgggt ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact
    10681 tactaaattc taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac
    10741 cacaaactta ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg
    10801 gtcaccgtta atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg
    10861 ttcaattttt gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac
    10921 gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc
    10981 agagaacaat gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa
    11041 tcttgtaact tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac
    11101 ttttttcgct aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt
    11161 cactacatcg cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt
    11221 cttcggatca agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt
    11281 cagcatcaat gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat
    11341 taggtctaat ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac
    11401 atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc
    11461 tgtaaatgag attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt
    11521 gatgttgaag tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca
    11581 gtcgtagttg ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt
    11641 ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt
    11701 gcggccgctt gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg
    11761 gcctgccaat tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga
    11821 atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg
    11881 aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat
    11941 attcatttta ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatatgt
    12001 aattaattat ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca
    12061 agggacgaaa tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg
    12121 aacatttaac gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt
    12181 aaagcatgta ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac
    12241 ttcgtttcga tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt
    12301 ctttgtcaac agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg
    12361 ctctaattcc gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt
    12421 tttctgcaat ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata
    12481 aggaatggga caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa
    12541 aaaattattt taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt
    12601 tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa
    12661 aaagcaaaag aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct
    12721 caaagctacg attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct
    12781 actgaaacac aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg
    12841 tctgatccta ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa
    12901 gccttccact ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc
    12961 aaccgttcga tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg
    13021 atccaaaaag acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa
    13081 aaaccattgg ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt
    13141 tttcctagcc gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa
    13201 gataaagaag aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa
    13261 catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga
    13321 gttttaggtt atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag
    13381 attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc
    13441 catacatggg gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat
    13501 gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca
    13561 tttggtgcag acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac
    13621 ttgaagagtc aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga
    13681 caattaggta cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa
    13741 tctgatttag acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat
    13801 gacaaagaag cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa aaagccgaac
    13861 tgacatttta tcgtcagttt cggctttttt atgctttatt tcttcttcgg tgcgattccc
    13921 ttgatcagga cttctgtcca ttctgctgta tatggaagca gttttgcgta tatctccggc
    13981 ttagaaattc cttctaccat tgcttgaaaa tagaggatca agaactcatc cgaatatttc
    14041 aaatccactt ttccttcttt ccgccctcga tggaaaagat caagcatgat actgaaactt
    14101 tcttgcgtat acgcatccat cgcgtcagaa aattcttttt tttctttttt tgtatagtat
    14161 gtcatcaaat cgatataaaa ttgtttgttc actcgattga gataacggat tttgttttca
    14221 ctcatggcaa tcagtgtttc ttcaaatgga agattttccg caatcaacgc tttcgcttct
    14281 tgccctaaat cattcataaa tgcaaaaaat acttcataaa tcagcccttc cttgctgcca
    14341 aaatatttaa aaatcgtcgc tttacttacc tgagcgttct cagctacctt atccattgtc
    14401 actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc
    14461 tttttctctt gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg taattttaaa
    14521 cttttataat atatatagtt caaatttatt ctgaggttta ttataactat ctattgagaa
    14581 agtatcaagc tagcaattcc ttgtttatga attcattgac agaaatttat aaaaagaata
    14641 ttatgtacta aaataataaa aaaagtttta taaaaaggta taaaaccttt ttattaaact
    14701 atttaatctt gtttagtata tatttttaga aaaaggagtg gggtttttat ggcagaaatc
    14761 gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa agatgtctct
    14821 ttcaccgtac attcaggtga agtcgttgga tttatcggac caaatggttc tggaaaatcc
    14881 acgacgattc ggacactttt agggattata aaaaaagacg gcggaacggc aacgattttt
    14941 gggcaagatg tatgggaaga tagtttatct atccatgaac gtctttctta tgttccaggc
    15001 gatatctctt tgtggggaaa tttgactgga ggagcgatca tcgatctctt catgaagctt
    15061 catggaaaag ggagtgctca aaaaaaagat gaactgattc aacgtttctc tcttgatcct
    15121 aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt gattgctgct
    15181 ttggctgttg attcggatct ctatttattt gatgagccta cttctggttt agatcctttg
    15241 atggaaacga ttttccaaga agaagttgaa aaattgaaag aacagggcaa atccatccta
    15301 ctttcttctc atattctcag tgaagtcgag cgactagcgg ataaagtcgt gatcatacgc
    15361 gaaggaacac tcgatgaaga aggaacactc gatgaattgc gtcatttgac tcgttcgacc
    15421 atcactatag aaacagaagc agagaatagt cacttagcac aattgaacgg tgtccatgac
    15481 tttgtgcaaa aaggtcacct cgctcaattt gctgctgata acgaagcttt cgatgtgatt
    15541 ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc gactcttgag
    15601 gatttattca tgagacatta tgaaggatga ggaggaagcg aaatgactga taaattttca
    15661 cgctggtcca cccttttttc tcaaaacctt agccgtgact ggaaaaagat cctgatatgg
    15721 ctagtcggtg tcactgcttt ttctggtggt tttgtccctg cgtttgaaga attgacttcc
    15781 ggacaaggat tagctggatt atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc
    15841 ggaccaactc cagcttcgaa tgctgctgag tatactttag gagcgatgta tgcaaatgaa
    15901 atgttgcttt tttgcagttt gtttgctgct gtgatctctg ttttacacgt ggtgagccaa
    15961 acgagaaaaa aagaagaact tggtctgatt gaacttattc gttcctttcg agtcggacgt
    16021 caagcaaact ctttagctgt catcgctgaa agtattctga tcaatttgct tttagcaata
    16081 tcgatcagct gtacgatgat ttttttccaa gcggattcga tcactgcaga aggtgcttgg
    16141 ctttttgggc tatctgtagg tagtatgggg attttgggga gtgccgttgc actgtttgct
    16201 gcacaactga tgccaagttc agccagttcg aatggtctgt ctttagcgtt tttaggtgga
    16261 ctttaccttc tacgagcaat aacagatgta gctgattcct ccctttcgat gtggaatcct
    16321 ttaggctggt cttatttgac ctatccattt acaactaaca actggattcc tttgctttat
    16381 ttgtttttgt tcagtctaat cttgataatc agttcatttg ttttagagga acatcgagat
    16441 ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta
    16501 tccttgcgtg gtttattatt ctatctaaat aaagggatga caatcagttg gcttatcact
    16561 tttggtttga tgggggcggc atacggctct atctacggga atatgcaaca gtttttagaa
    16621 agtaacgact taatgaaaca gatgtttatc caatctggcg tctcaattga aaaaaacttt
    16681 acaagcacga tcatggtagt cttgatttgt ctagctgcta ttttaccaat tgctagcatc
    16741 aatcgtttat ttacagaaga aacccacgga cgtatgaatc agctgttcgc aacaaaaatt
    16801 tctaggaacc aactttattg gaccaatatg ttactagcta ttctatttgc tatattggca
    16861 atcctgatta gttctggtgc actagggctt tctgctttgg cttcgatgaa ggatgcgaaa
    16921 gacatggatc tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg
    16981 tttatcggaa ttagtggctt tctattcgga tggcttcctc gaatcggtaa acttgcttat
    17041 atctacttag cttactcctt tgcactcaat tactttggcg gaattttgga tttaccagaa
    17101 tggttttcca aaacagctgt ccctagctgg gttccacgga tgccaaacga ttcttttgat
    17161 ctttccgtgt tccttacgtt atcatgtatc agccttgcac tgttcgtcct tggttctatt
    17221 ggttataggc gaagagactt actagaagga taataaaaaa gcatgtacaa tttcatgcta
    17281 gcctccaaat ctgggtcaaa aatccaacat ttggaggtca tttttttatt tacaagtctt
    17341 ctaactgctc tttatcaatg atccagactt ctcgattctt tctttttaca taaccattct
    17401 cctctaataa tttaaatttt ctagataaag tttcgggggt cgtaccgata aatgcagcaa
    17461 gttctttcat tttcattggc aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa
    17521 gcagatagag agcgactcgt tcttctactt tttccatcat caaaaaagtt gtctgctgct
    17581 gtacttttgt taatttctca acgttgattg ctaaaagttt cagactaagg gcgggataat
    17641 gcagtaagag ttccgagaaa tctttttgtc tcaatatgca aatttccgtt ttttctagtg
    17701 cttcgccaaa taaagactca tttggaacgc ctagcaactg gctctctccc tcatatccgc
    17761 ccggctctat tactcgtaaa agctgttcct ttccgctagc agataattga tacactttca
    17821 tactgccgga ggccacgatc actaattgag gttctccatt cggcgcaaga atctgttctc
    17881 ctttttccac atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta
    17941 agtgattgaa caaaggaaca aatgaaacac ataaatgatc atgactcata atctctcctc
    18001 cttctaaaca gctatttcta ataaagaaac ataacttccc ttattccaac tgataatttt
    18061 cttttatcat agcataaacg tcaagcgaac cagataccag caaatagagt ggatttcttt
    18121 ttgtcccttg ctttattttc cttcttttga gaccttaagt aattgattcc tatcaatttt
    18181 ttaaatattt actttttgaa taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg
    18241 tttgtttttc gcattgctta tttcgtgttg tctattgaaa tttttccgca cctagctttt
    18301 gatatagtgg tgctagttct tgtaaggttt cagcggttat ttgctcaacg tgttcgattt
    18361 cttgcggata gaagaatagt ttacccattt gaaaggttcg gtctctcatc gcttttttca
    18421 actcttcttg tttctttgta tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt
    18481 catgttctgg ttgcggccat atgtaataat ccttgatatc gactgttttg ctccactctt
    18541 tcagtatcgc aagcaactgg ttatacgctg caatgctacc atatcgttct tcgcttttat
    18601 aatgttggta catcaaatag atttggtatt gtttttgatt caacaaaact gctagacagg
    18661 cattctcgtc ttgacgagat tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc
    18721 agccattcgt ccagctttcg attttaggtt tgctcatgcc gtacatatca ggcaaaagtg
    18781 cagctgtctg gagctgaatc tctttccatt tttgccagtg ttctttatat tctttcttga
    18841 tttgttcaat cttatctgtt ggatggctct ctttgagctg tttgaatgcg tagattggtc
    18901 gatcaaacaa ctgataatct gattttccaa gttccattgt ttcgcctctt tccgttatct
    18961 tttgtattct acctgaattt tgcgatatgg acaaatagag gttgtgaaaa agctgttctg
    19021 catcaagtca taagaacagc caaacaaaaa actgcgatga gcatcaccgc agtttta
//
LOCUS       DAYYAJ010000049        19045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000049 DAYYAJ010000000
VERSION     DAYYAJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.049"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..822
                     /locus_tag="ACHATZ_001433"
     CDS             70..822
                     /locus_tag="ACHATZ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8403967.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            819..1733
                     /gene="pfkB"
                     /locus_tag="ACHATZ_001434"
     CDS             819..1733
                     /gene="pfkB"
                     /locus_tag="ACHATZ_001434"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8403968.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1751..3682
                     /locus_tag="ACHATZ_001435"
     CDS             1751..3682
                     /locus_tag="ACHATZ_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8403969.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3793..5352)
                     /locus_tag="ACHATZ_001436"
     CDS             complement(3793..5352)
                     /locus_tag="ACHATZ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8403970.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5507..7741
                     /gene="pcrA"
                     /locus_tag="ACHATZ_001437"
     CDS             5507..7741
                     /gene="pcrA"
                     /locus_tag="ACHATZ_001437"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8403971.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7758..9794
                     /gene="ligA"
                     /locus_tag="ACHATZ_001438"
     CDS             7758..9794
                     /gene="ligA"
                     /locus_tag="ACHATZ_001438"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8403972.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9900..10205
                     /gene="gatC"
                     /locus_tag="ACHATZ_001439"
     CDS             9900..10205
                     /gene="gatC"
                     /locus_tag="ACHATZ_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8403973.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10205..11677
                     /gene="gatA"
                     /locus_tag="ACHATZ_001440"
     CDS             10205..11677
                     /gene="gatA"
                     /locus_tag="ACHATZ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8403974.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11674..13104
                     /gene="gatB"
                     /locus_tag="ACHATZ_001441"
     CDS             11674..13104
                     /gene="gatB"
                     /locus_tag="ACHATZ_001441"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8403975.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13114..14169
                     /locus_tag="ACHATZ_001442"
     CDS             13114..14169
                     /locus_tag="ACHATZ_001442"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8403976.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14280..15656
                     /gene="rlmD"
                     /locus_tag="ACHATZ_001443"
     CDS             14280..15656
                     /gene="rlmD"
                     /locus_tag="ACHATZ_001443"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8403977.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15865..16701
                     /locus_tag="ACHATZ_001444"
     CDS             15865..16701
                     /locus_tag="ACHATZ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8403978.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            16850..17071
                     /locus_tag="ACHATZ_001445"
     CDS             16850..17071
                     /locus_tag="ACHATZ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403979.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            17072..>19045
                     /locus_tag="ACHATZ_001446"
     CDS             17072..>19045
                     /locus_tag="ACHATZ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFM8403980.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYW"
BASE COUNT         6425 a         3354 c         4156 g         5110 t
ORIGIN      
        1 gtttttgact gatttatatt gcgagtagaa aaaaaatatg ctattatttg tattgaggtg
       61 atcgtgaaaa tgcttacaga ggaacgtaga caaaaaatcc tagacttgct tgaacagcag
      121 aaaatcgtga aatcacaaga tcttgtaaac ctttttaatg cttctgaatc aacgataaga
      181 cgtgatttac aagagctgga gaatagtggg tttctagaaa gagttcatgg cggagctaag
      241 aaagagcaat tattgaattt tgagcagaat atgagtgaaa aatcactcaa aaacgttcac
      301 gaaaaacagc aaatcgccaa atcggcagct gaaaccatta atgacgaaga tgtcatttat
      361 ttagacgctg gctctactac gttagaaatg attccttttc tgcaaggaaa acagatcaca
      421 gtagtgacaa attccgttca acatgcggca aatttagttg atttggagct tgctaccatt
      481 attttaggtg gaacgataaa gctggcaact aatgctgttt taggatctaa tgcgatagaa
      541 caactaaaaa attttcattt taataaagca tttatgggaa tgaacggtgc ccatcttgaa
      601 caaggattca cgactccgga cccagaagag gcagctgtta aacgtttagc catgaaaaat
      661 tccgaagaga catttgtatt gattgatcat accaaattca accagttgtc cttcacctct
      721 gttgcacctt tggaagcagc cacgatcata accgagcgtt gtccattaga attcgttaat
      781 gaattccgtg aaaagacaac aattaaggag gcaatacaat gatttacaca gtaacactta
      841 atccatcaat tgattatatc gtacgcgtag atgggttaaa actaggcgat ttgaaccgaa
      901 tgaaggaaga cttcaagtta cctggaggaa aagggatcaa tgtttcccgt attttgaaac
      961 ggatcgattc tcaatctaca gcactagggt ttttaggagg gttcactggc gaatttattt
     1021 cgaagtggct tgaaaaagaa caaatccagt ctttgttcac aaccgtaaaa gaagatacaa
     1081 gaatcaatat caaactaaaa tcagatactg aaactgaaat taacggtttg ggcccatcta
     1141 tttcagatga agaaatcgat gagttgaagc aagcattagg aacagtagga caaggagata
     1201 tcgtcgtatt gtcaggaagc gcgccggcta gcctgcgaaa aggattttat caagaactga
     1261 ttgatatcat tcgagaaaaa ggtgcagatt tcgtgattga tacaacaggt gatgacctaa
     1321 aaaatgcact tgttaagaaa ccattgttga tcaaaccaaa caatcacgaa ttagctgagc
     1381 tttacaatac aaccttccaa tctgtagaag aaatcattcc gtatggtaaa aaattgctag
     1441 ctgaaggggc acagaatgtg ctgatctcaa tggctggaga cggtgctttg ctcttcacgg
     1501 aacaaggaac ttataaaacg aatgtattag ttcgcccatt gaaaaattca gtaggtgcag
     1561 gggattcaat gattgccggc tttattgggg cttacagccg tacgaaagat ccaatcgaag
     1621 cctttaaatg gggagttgct tgcggaagcg caacagcttt ttctgatgac ttggcagctc
     1681 gcgaattgat tgatgagttg ctgccagaag ttgaaatcac aaaaattgat tagtttcaag
     1741 gaggataatc atgaatatca aggatttaat ggtcaaagaa gcgatgatca tggatttgca
     1801 agcgactgat aaaaaaggcg caatcgatga aatggtccaa aaaatgtttg atgccggccg
     1861 gatttccgat atcgaaacat ataaagaagg aattctagcc cgcgaagcac aaacttctac
     1921 aggtcttggt gatggaatcg ccatgcctca cgcgaaaaac agtgcagtta aagaagcaac
     1981 agttttattc gcaaaaagcc aaaaaggagt agattatgaa gccttagacg gacagccaac
     2041 ttatctgttt ttcatgatcg cagctccaga aggcgcaaac gacacacatt tgcaagcatt
     2101 agcagcacta tctcgattgc tgattgatgc agatttcgta ggaaaattaa aagaagcaaa
     2161 tacaccagaa gaagtccaag acttgttcca aacagctgaa gagcaaaaag aagcagaaga
     2221 aaaagcagaa caagaaacac aagctgcaga agcttctgct catccagaaa gaaaattcgt
     2281 tgttgccgtt acagcttgtc caacagggat cgctcacact tacatggcag aagatgctct
     2341 gaagaaaaaa gcgaaagaaa tgggtgtgga gatcaaagtc gaaacgaacg gttcagaagg
     2401 aatcaaaaac cgattgactg ctgaagacat cgcccgtgca gaaggtgtaa tcgttgcagc
     2461 agacaaaaaa gtagagatga atcgctttga cggaaaagaa ctgatcaatc gtcctgtcag
     2521 tgatggtatc cgtaaaccag aagagctgat caatctagcc cttagtggta ctgcaccagt
     2581 attccatggt gatggaaaag aaagcagttc agaagaagga aatgcggatg gtactatcgg
     2641 acaaagaatt tacaaagatt tgatgaacgg cgtatcacac atgttacctt tcgttattgg
     2701 tggaggaatc gctattgcgc tttctttcat gatcgaccaa tttattggcg tgccacatga
     2761 tcaattatca aatctaggga attacaatca agctgctagc tggttcaacc aaatcggaca
     2821 agctgcattt ggtttcatgc tgccggtatt agcaggattt attgcttcaa gtatcggtga
     2881 ccgccctggt ttgatcgttg gatttgctgc tggtgcatta gctaatacag gcggtgcagg
     2941 attcttaggt gcactgattg gtggattctt agctggttat gtcatcgtat ttttacgtaa
     3001 attattcaag aacttaccaa aatcattgga tggaattaaa acgatcctct tttatccagt
     3061 gtttggctta ctgattactg gtttcttgat gttgctggtc aatgtcccta tgaaagcaat
     3121 caatgatgca ttgaacggtt tcttgacaag tatgagtggt tcgaatgctg ctttacttgg
     3181 tgcattatta gctggaatga tggcggcaga tttaggcggt ccaatcaaca aagccgctta
     3241 tgtgtttggt acagcaactt tagctacgac tgttgcaact ggcggaagtg tcgtgatggc
     3301 ttctgttatg gctggaggaa tggtacctcc attggcaatt ttcgttgcta cacgtttgtt
     3361 taagaataaa ttcagcaaaa cagatcaaga tgctggtttg acgaacatcg tgatgggact
     3421 atcgtttgtc acggaaggat cgattccatt tgcagcagct gacccgattc gtgccattcc
     3481 aagttttatt attggttctg ctttaactgg cggattagtc ggagctttcg ggatcaaatt
     3541 actggctcct catggcggga ttttcgttgt attcttactg agtcacccgg tcatgtattt
     3601 ggtgtttatt gctatcggag cagtcgtatc tggtgtgatt tatggtttat tgagaaaagc
     3661 tcctgcagca gcagaagcat agaaataaaa aagaagaggc tggtttttcc cagtctcttc
     3721 tttttttata gaatcatata gaatcaagta gtgtagatga atagttgttg taaaatagtt
     3781 tttccatgtt cgttaaaatg attgcttcaa atttgtgata gaagatttag cctcgatttc
     3841 ctgcttgatt cttggcaagt ttgcttcatc agtcaagata accaaaatat ctcctacatt
     3901 catgatcgta tcaccgtgag tcaagatttc ctgatttcct ctgcgtatag agattagcag
     3961 catattttta ggccaattga agtctcgtac cattaatccg tctaaagaac tctctgccgt
     4021 tactggaaaa tcaaaagttg tccttgcacc ttttagtgag gcggtctgag tatttccaag
     4081 tagacgttcc aataatgatt cgtaaattgg attaccgccg agaaggtcat tgacgatata
     4141 agcaacaaga gcagccaatc ctaaaggcat cagatgatta agagagccaa ccatttctgt
     4201 cactaaaata attgctgtca gcggtgcttt tccaatcgct gtgaaataac cagccatggc
     4261 aataacgaca aaatccttga tgtagattgg atccatcccg aaatcaacca taatgtttcc
     4321 ataaaagcta ccgattaaag cccccaatgt caatatcgga aggaaaatcc cccctggaag
     4381 accaccaccg taagaaatca tggaaaaaac gaatcgaaga acaaacaaac cgataatcat
     4441 aatgattggc aaggaagcat gtccaaaagc taaaacgatt tgattgcctc cgcctagagt
     4501 actaggccat aaataaccaa ttggcaaaat cagcaagaaa ggaacgaacc cgtgaaaata
     4561 agctggtaaa ggcaatcggt tgtaaaattt tggcaaagcc agcaagacct tctgataaat
     4621 aaaacctaag atgcctagaa gtactcctaa gatcactaag gaaccataat atttcaacgg
     4681 caaagaagga aggtcaccta gatataaaac aggtctcaag ccgaaaaagt aaagagagac
     4741 gaagttagca gtgattgctg aagcaaatga tgtcagccaa acaagtggag aaaaggagtg
     4801 atggatttct tctaaaacaa agagtaatcc tgcgatagga gcattgaatg ctgctgcaag
     4861 tccgctactt gcaccgctag aaattaaaat cttttcttct gaagatggac tccgaaacaa
     4921 ttgtccagct ccttgtccaa tggatgcgcc aagctgtata gaaggtcctt ctctacctag
     4981 aaaaaggccc gcaccaactg aaagtacacc gccaatgaac tttttccata aaacagagaa
     5041 ccagttcatt gataattccc cgcgaagctg tccctctact tgtggaatcc cgcttccctt
     5101 gatatccggt tcatttttga ccagataacc cacgaaaaat gctgcaagga tgcagcaaat
     5161 agctaggcca attaagtaaa tcggctgttc ccgactcatt tcatagatac ttcgaactaa
     5221 atcgcttagc ttttcgatag ataatcgaaa caggctaacg acgattcctg tcagcagtcc
     5281 aacaaatatt ccttttagaa taaaatatat ctgagtctta tctaatttct tgatttttcc
     5341 ttttttttcc attcaaacgc ctcgctttct tttaatttag cgcgaaatca acgaattgac
     5401 aaggaaaaat ccataaaaag aaacaaatga ttttgaacta gctagtttaa tgaaagccaa
     5461 gaaaatgcta taattaacaa gattgataaa agaaaggatg ttgttaatgt catccaaagc
     5521 tgaattatta aacgggatga acccccgtca gaaagaagct gttcttcaca ctgacggacc
     5581 attactgcta atggcaggtg caggaagtgg aaagacgaga gtgctgacac accgcattgc
     5641 ctatttgatt gaggaaaaag aagtcaatcc ttggaacatc ctagcgatta cttttaccaa
     5701 taaggctgca aaagaaatga aagaacgtgt caatgcgatt ctcgcatctg gaggcgaaga
     5761 tgtctgggta tcgacttttc actcgatgtg tgtgcgtatt ttaagaagag atgttgattt
     5821 tatcgggtat aaccggaact ttacaatcat cgattcttct gaacagctga ccttgatgaa
     5881 acggatacta aaagaactga acattgatcc gaaaaaatat gatcctcgaa gtatattagg
     5941 aacaatctct caagcaaaaa acagcctgca gacccctcag gattttgcaa aaatgcaagg
     6001 cagttattac gaagagattg cggcaaaatg ttatgctgct taccaaaaag agcttcaata
     6061 taatcaatgt atggactttg acgacctgat catgaatacg attcgattat ttgaagaaca
     6121 tcctgattca ctcacatatt atcaaaacaa attccattat attcatgtag atgagtacca
     6181 agatacaaat cacgcacagt atacgctagt caatttattg gcaggtagat ttaggaacct
     6241 gtgcgtcgtg ggagatgcag atcaaagtat ttacggctgg cgcggtgcag atatgcagaa
     6301 catcttggat tttgaaaaag attatccaga tgctgcagta attttgctag aacaaaacta
     6361 tcgctccacg aaaaacatcc ttagcgcagc aaaccaagtc attgaaaaca atagcaatcg
     6421 aaaaccaaaa aacttgtgga cagaaaacaa agaaggaaac aagatcactt actaccgtgc
     6481 agataatgaa cgagacgaaa cccgttttat cgtagatcgg atgcaagaag agattcgaag
     6541 caatcatcgg aattacggcg actttgccat cttgtaccgg acgaacgcac aatctcgtgt
     6601 aatggaagaa actctgttga aagcaaatat tccttataaa atggttggag ggcacaagtt
     6661 ctacgatcgt aaagaaatca aagatatttt agcgtatttg aatgtgctgg caaatcctca
     6721 agattcaatc agttttgagc gtatcgttaa ttcgcccaaa cgaggcatcg gaccaggatc
     6781 tatcgaaaaa ctacgcagtt ttgcttctct tcatgaatgg ccactgcttg aagcagcgca
     6841 aaatgtggat ctggcaaata tcggtggaaa agcaggacag caattaggtg catttggaga
     6901 gatgatccaa gaagtcactc agatgatccc atacttgaca gtgacagaat tgacgaaaga
     6961 ggttttggat cgtagcggat atctagaaga cttaaaaatc caaaatacat tagaagcaca
     7021 agcacgcatc gaaaacttgg aggaattttt aacggtcaca caagaattcg acaaacaatt
     7081 cgaacagcaa aatgaagaag acgctgatgc accagaagaa aaactaacgg tcttcttaaa
     7141 tgatctggca cttgtttccg atattgacaa tttagaagaa gatgcttccc aagtgacatt
     7201 gatgactttg catgcagcca aaggattaga attcccagtt gtattcctga ttggtctaga
     7261 agagggggtt tttcctttat ctcgagcatt gatggaagaa agcgaactgg aagaagaacg
     7321 ccgtctcgca tatgtgggga tcacgcgagc agaagaagcg ttgtatttaa caaatgcttt
     7381 ctccagaaca ttatatggac ggacgcaata taaccgacct tctcgttttg tagaagaaat
     7441 cgatcaggag ttactggaaa tcgaaggcat gcgtcctacg cctaaaaaaa cacctgtatt
     7501 cgctaaaaaa acagcatata gctataaaca gccagagaca gcggttgttc cctctaaaag
     7561 tgctactgga ggggaaaata acagctggaa acctggagat aaagtcaaac acaaaaaatg
     7621 gggtctagga acagtcgtcc gagtaagcgg gacgtcgaag gatctagaac tagatgtggc
     7681 tttcccaagt caaggagtaa aacgtctttt ggctgccttt gcgccaattg aaaaagctta
     7741 gtagtggagg gtaaatgatg gaacatgaaa tgacgattga cgaagctgct aaacgagcag
     7801 aagaacttcg tacaagactg aaccaatggt ctcgcgaata ctacgtagaa gataaaccaa
     7861 cagtggaaga ctatgtttat gataaagaat acgcagaatt agtagcaatt gaggaacaat
     7921 atcctgattt gatcacgtct gattctccca ctcaacgagt aggcggaaaa gtattagaag
     7981 gttttgaaaa agtcactcat gatattccgc tttacagttt gaacgacgtt ttcagcaaag
     8041 aagagctgat agcttttgat caacgtgtac aaaaagcagt gggacgtgta gttgactatt
     8101 gctgtgaact aaaaatagat ggactttccg tttctttgcg ttatgaagat ggaaatttcg
     8161 tgagaggtgc gactcgcggg gatggaactg taggagaaaa catcactgaa aacctaaaaa
     8221 ctgtccggtc ggtcccaatc aaattaaaag aaccaatgaa catcgaagta agaggcgaat
     8281 gtttcatgcc aaaacggtca tttgtccaat tgaaccaaga cagagaagca gagggaaaag
     8341 atatctttgc gaatcctcgg aacgccgcag ctggttcttt gcgtcagtta gattctaaaa
     8401 tcacagcgaa aagaaatctg gacacttttt tatacacagt agctgatttc ggtcctatgc
     8461 aagcaaaaac ccagtatgat gcgctagaag agctagaaaa aattggattc cacacgaatc
     8521 gggaaaaacg tctatgtcat tcgattgatg aagtgtggtc atacattgaa gaatatcatg
     8581 acaaacgtgt ggatttgccc tatgaaatcg atggaatcgt catcaaagtg aatgaattct
     8641 cgctacaaga tcaacttggt tttactgtca aagcaccgag atgggcagca gcttacaagt
     8701 ttccgccaga agaagttgaa acgctaatcg aaaacatcga atggacagtc ggtcgaacag
     8761 gggtagtaac gccaacagct atcatgacac cagtccgagt agcaggaacg acagtaagcc
     8821 gtgccagcct gcataacggt gattatatta agttgaagga tatccgcttg aaagatacag
     8881 ttctgattta taaagcggga gacatcatcc cagaagtttc gcaagtcgtg ttagataaac
     8941 gacctaaaga cagcgaagaa taccaattgc ctacacattg tccagtttgt ggaagtgaac
     9001 ttgtccattt ggatgaagaa gtagccttac ggtgcatcaa tcctaaatgc cctgctcaaa
     9061 tgaaagaagg tttgaaccac tttgtttcaa gaaatgcgat gaatatcgat gggttggggc
     9121 cgcgcgtact agaacagatg tatgataaaa aactagtagc agatgttgct gatctttaca
     9181 aattgacaga agaagagtta ttgacgttgg acaaaataaa agaaaaatca gcaaataata
     9241 ttttgacagc aatcgacaac agcaaagata attcggtcga gcggttgatt ttcgggttag
     9301 gaatccgaca tgtcggagcg aaagcagcaa aaattttagc agaacatttc ggtgatctag
     9361 aaacattgag taaaagtgac tatgagtcga ttattgcgct tgatacgatc ggtgatatca
     9421 ttgccgacag tgtagtcact tatttcagta acgaagaagt ccatgaattg atgaatgaat
     9481 taaaacaagc aggcgtcaat ttcgaataca aagggcttcg aaatgcccaa ttgcaagaag
     9541 tcgagtctcc ttttaaagaa aaaacagtag tccttactgg taaactaaca cgatttactc
     9601 gtgaagaagc aaaagagacg atcgaaaatc ttggcgggaa agtgactggt agcgtatcga
     9661 aaaaaacgga tatcgtggta gcaggagaag atgctggcag caagctgacc aaagcacaag
     9721 aattaggaat cgaagtttgg acagaagatc aaatggcaga tgcattggct aaaagtcgct
     9781 cagtagaaga atgaaaaatg agttttttca gacaaatttc aataaacaat ttcagacacg
     9841 gcactcttgt gttaaaatag agagcaaagt ttttaactcg tttagaaaga aggggtagta
     9901 tggcgattag tgaagaacaa gtgaaacacg ttgccaaatt agcaaaactt gcgttcgcag
     9961 atgaagaact tgcaagtttt accgaccaat taggtaaaat catcgatatg gttgaaatgc
    10021 tggaagaagt agatacagaa ggtgttccat ttacgtcgaa tgtggttgaa acagtaaacg
    10081 tgatgcgaca agatcgggcg caagctggat ggaaccgcga tgaattattg aaaaatgtgc
    10141 cggaaacaga agacggcttt atcaaagtac ccgcaatcat cgataatggg gaggctggcg
    10201 cataatgaca gaattacaca atcgttctct agaagaactg catcatatgc ttgtatcgaa
    10261 agaaatcact gtacaagatt tgacaaaagc aacatttgaa cgaatcaaag aaacagagcc
    10321 tgaggtcgaa gcattcatta cattgaacga agaaaaagca ttggaacagg caaaagcttt
    10381 agatgaaaaa gggattgatg aaagcaacgt ccttgctggt attccaattg gtatcaagga
    10441 taatatcgta acaaaagata tcttgacaac cgctgcatct aagatccttt acaattttga
    10501 accaatttat gatgcaacgg tcatggataa aatctatcaa gcagatatga tccctgttgg
    10561 taagctaaac atggatgaat ttgctatggg tggaagtaca gaaacttctt atttcaagaa
    10621 aacaaaaaat gcttgggacc caacaaaagt acctggtggc tcttctggtg gttctgcagc
    10681 agcagttgca gctggacaaa ttccagtttc tttaggtagt gatactgggg gaagtatccg
    10741 ccaaccagca gcttttaacg gcatcgtagg gatgaaacct acttatggcc gtgtctcacg
    10801 ttttggtttg attgcctttg cttcatctct tgaccaaatc ggaccaatga cccgtacggt
    10861 gaaagataat gcattagcat tgacagctat cagcggttat gacgaaaaag acggtacttc
    10921 ttcaggcgtt tcagttcctg attttgcaga gggactgaca ggcgacatca aaggaatgaa
    10981 gattgcttta cctaaagaat acttaggcga aggtgtggcc cctggcgtaa aagaagctgt
    11041 attgaaagca gcagaaacat tccgttcatt aggtgctgtt gtggaagaag tcagcttgcc
    11101 tcattcaaaa tacggtgtgg ctgtttatta tatcattgcc tcatcagaag caagttccaa
    11161 cttgcaacga ttcgatggta tccgctacgg ttatcgctca gaaaatgtga aatcattaga
    11221 tgatgtatat gtcaactcac gttcagaagg attcggtgaa gaagtaaaac gccgtatcat
    11281 gttaggtaca ttctccttaa gtgctggtta ttatgacgct tacttcaaaa aagcgggaca
    11341 agtccgtaca ttgatcaaac gagattttga aaaagtcttt gcagattatg acttgatcat
    11401 cgggccatct tcaccaacag ttgcattcgg aatcggtgag aacattaacg atccaatcac
    11461 aatgtacatg agtgacatct taacgatccc tgtcaacttg gctggacttc caggtatgtc
    11521 tgtaccggca ggtttatcag aaggattacc agttggattg cagatcatcg gtaattattt
    11581 tgatgaaaaa acgatgtatc aagcggcata tgcgtttgaa caagcaacag aattccataa
    11641 acaacaacca gctattttag gagggaaaga cgaatgaatt ttgaaacagt cattggacta
    11701 gaagtccacg tcgagttaaa aaccaactca aaaattttct ctccagcgcc agctcatttt
    11761 ggtgcagaac caaacagcaa tacaaacgtg atcgactggg gttatccagg cgtgctgcca
    11821 gtaatgaata aagctgcgct ggaattcggg atgaaagcag ctttggcatt aaactgtgaa
    11881 atctctaaag atactcattt tgaccggaaa aattactttt acccagataa tccaaaagca
    11941 tatcaaattt cccaatttga tcaaccaatc ggtcatgacg gatggatcga aatcgaagta
    12001 gaaggtaaaa agaaaaaaat ccggatcgaa cgtgtccatt tagaagaaga tgctggaaaa
    12061 aacatgcatg gtattggcgg ttattcttac gttgatttga accgacaagg cacgcctttg
    12121 atcgaaatcg tttctgaagc tgatatgcgt tcaccagaag aagcatacgc ttatctagaa
    12181 gcattgcgat caattatcca atttacagaa gtcagcgatg tgaaaatgga agaaggctct
    12241 atgcgctgtg atgccaatat ttctcttcgc ccttatggac aagaagagtt cggtacgaaa
    12301 gcagaattga aaaacttgaa ctctttatct ttcgtgaaaa aaggtttagc ttttgaagaa
    12361 aaacgtcaag caaaagttct tctttcagga ggagaaatcc aacaagaaac acgccgtttt
    12421 gacgaaacaa cgaataaaac agttttgatg cgtgtcaaag aagggtctag tgattatcgt
    12481 tacttcccag agcctgatat cccgaaattc gtgatcgatg acgaatggat tcaaaaagta
    12541 aaagctagtt tgcctgagat gccagcttct cgtcgcgaac gctatatccg tgagtttgga
    12601 cttccagagt atgacgcaat ggtattgaca cttacaaaag aaatgtctga tttttttgaa
    12661 gctgttttgg aaaatggtgc agatgccaag caagcatcta actggttgat gggtgaagtt
    12721 tctgcttact tgaacagtga gaaactggaa ttagctgaga caaaattgac acctgagaac
    12781 ttagcaggaa tgattcgttt gatcgccgat ggaacgatca gctcaaaaat cgccaaaaaa
    12841 gtcttccgtg aattgatcac aaatggcgga gaagcccaag aagtagttga aaaaaatggt
    12901 tgggtgcaat tatctgatcc gaacaaacta ctgccaatca ttaatgaaat tttggataac
    12961 aaccaacaat ctgtcgatga tttcaaaaat ggaaaagatc gtgcggttgg cttcctagtc
    13021 ggtcaaatca tgaaagcaac taaaggacaa gcaaatcctg gtgtcgtgaa taagctactt
    13081 aaagatgaat tagcgaaacg atagaggctg aaaatgaaaa aagcacgcgt aatttataac
    13141 cccacctcgg gaaaagaatt attgaaaaaa aatttggcgg atatcctaca ggcattagaa
    13201 gaagcaggat ttgaagccag cgcctatgca actacaccag aaccagattc agccaagaat
    13261 gaagcatgta gagtcgcaga actaggtttt gatctagtag tggcagctgg tggcgatggc
    13321 acaatcaacg aagtggttaa tggaatcgct ccgcttgaac accgtcctaa aatggccatc
    13381 atcccagctg gtacaacgaa cgactatgct cgagcattaa aaattcctag agataatatt
    13441 aaagcagcag cagaagtgat aaaaaaacac caaaccgtaa aaatggacat cggcctttct
    13501 gataacagtt acttcatcaa tatcgctgca ggtggctatt tgacagaatt gacttatgaa
    13561 gttccatcag aactaaagag tatttttggt tatcttgctt atcttgcgaa aggagcagag
    13621 atgctccctc gagtaaagcc aatcaaaatg cgcttgaagt atgatgaagg ggaatatatc
    13681 ggaaatgctt cgatgttttt cctagggctg acgaactctg ttggaggatt cgaacagatc
    13741 gcgccagatg ccaaattgga tgatgggaag ttttctttaa tcattgtgaa gacagcaaat
    13801 atctttgaga tcttgcatat tgcagcactc atgctgaatg gcggtcgaca tgtcgatgat
    13861 ccaagaataa tctataccaa aacaagcagt ttgttcgttg aaacagaaga tgatatcaat
    13921 cgaccagtaa tgatcaactt agatggtgaa tacggcgggg aagcaccgat gcatttccaa
    13981 aatcttcatc aacacattga gttctttgcc aataccgatc agattcctga tgaagcaatc
    14041 acaggtacag atgaagaaga attagaggtt gtcagcaaag aattcgtaaa agaagtcgaa
    14101 cgtttaacgg atgaagatat cgacggtgac ggaaaaatag cagacgaaac acctaatcaa
    14161 aagaattaat caaaagccgg tgctgaacgt agtaaggtca gtgccggttt tagttgtgta
    14221 attagctgat ttatagtaga gttcagtagg attgaaaaat aggcttaaag gagcaaaata
    14281 tgacagaaca gcaaatagta aagaaaaacg aacgatatac agtcgaaatc gtcgacttga
    14341 gttatgaagg aatgggggta gcaaaaatcg atggttatcc attatttatc gaaaatgcac
    14401 tccctggaga agaagtagaa atactagtag taaaagcagg caataagttt ggatatggaa
    14461 aagtcgaaac atttattaca caatcacctg atcgtcagcc tgtagaaaac aatgcattac
    14521 ttcgcatagg tatcgcacct ttagcccatt tgaagtatga acaacagctt gtcttcaaac
    14581 aaaaacaagt agagcatgta atggcgaaga ttgctaagat gccagaagtt cctgtacttc
    14641 caacggtcgg aatggcagaa ccattcggct accgtaataa agcacagatc cctgttcgcc
    14701 gtatcgatgg acagttagcc accggttttt acaaaaagaa tagccatgat ctagtcccta
    14761 tcgaagattt ttatatccaa gatcctgtca tcgatcaagc aatcaaagta gtcagagata
    14821 tcttgcaacg gttccaagta cgaggatata atgaagaaaa aaatgaagga cagcttcgtc
    14881 acatcattat ccgtcgtggt cattattccc atgaaatgat ggtcgtttta gttactcgta
    14941 aagaaaaatt ctttaaatcc aaggaaattg ctgaagctat tcatgaagaa ttgccagaag
    15001 tggtttcggt catccaaaat atcaatgaag aaaaaacaaa tgtgatttta ggagacaaag
    15061 aaaaagtttt atatggcaga gactatatcg aagatcagct attaggaaaa acctatcgaa
    15121 tctcagcaaa gtcattttat caagtaaata ctcaacaagc agagtatctg taccaaacag
    15181 ccatcgagtt tgccgagtta gataaaaaag acattgtgat cgacgcttat tcaggaatag
    15241 gtacgattgg cttgtcttta gcagacagag tggataaggt gtatgggatg gaagttattc
    15301 cagaagcaat tgaagatgca cagttcaatg ctttaaataa taaaatcgaa aatgttcatt
    15361 atgaagtcgg aaaagcagaa aaagttatga aaaaatggca agaaaaaggg atcaaaccat
    15421 ctgtgatcgt agttgaccct ccacgaaaag gattagatca tcattttatt gaatcagcaa
    15481 tcgaaatgtc accagaacga atcgtctata tctcatgtaa tccggcaact tttgcaagag
    15541 atgctaagtt atttgcagaa gcaggttatg ccgtgaagaa agttcagcct gtagatcttt
    15601 tcccgcaaac ccatcacatc gagtgtgtag gactgcttac acgtgtggaa cgttgatatg
    15661 acaaggatta gagcgattcg ggatggtttc cggattgctc ttttttcgtg ttgaaatgac
    15721 gatttttggg tgagcgtaga gttgatggaa atgctatgga gctttgagtt gataggagtt
    15781 acaccgtaga cttgataggt tagatgattt tgataacggt tacgtgatat aatatattat
    15841 cgagttttca agggagacaa acttatgaag caaattttta tattttcaga tacacagaaa
    15901 gataatattt cttattttga acgcgagcca gcaatcttgg tttcaatgag tcgtagtgat
    15961 ttgccgtatt tgaaggaaca ggatttttca aattatccag ctgtttatgt tttgattggt
    16021 ggtaataagc gttacgtggg tcaggcagca gggcaatcaa tttcacttcg tttatctcaa
    16081 cactttctca aggaatataa agcttgggtt gaatcagttt tattctttac aagagcagat
    16141 gggaaaatgt caaaagcaga tacagattat cttgaaaaac gacttatcca agatttccaa
    16201 gaaaaatctg actatgaaat gatgaattca actgctggta atacttcgta tattgataaa
    16261 cttcaaaaag caaaatcgga tcagctatat gacacagtgt ttgaaattat agatgaaatt
    16321 gcaaatatcg atttgtttgg tacttccgaa gacagtgaaa cccaaattta tgaatttagt
    16381 tcatctggac aatatgaaat tgagtatgat gggaaaaaga tatcttcgaa atctgctaga
    16441 ggtttattgg ttgattttgt ttctcagcta tttgcggatg gaaactacaa caatcaactg
    16501 caagaattaa ttgtcgatga gacgccgtcg ttcgctataa ttttaggacg taaaccatcc
    16561 atgtataacg gaagaccaaa ttcagccagg gtagctgatg gagtatgggt ttatgcaaat
    16621 ttcaacaaaa agaatgtcag aatcaaaatt gataaactag ctcgtcaatt ggggattccg
    16681 gttaaaataa agtggaatta atatttgtta tctcgccttt agtaaatcta tgctgaaggc
    16741 ttttatttta gaaaaatagt ttgcgttcgc gaaaatattt catgtttcgt ataataatga
    16801 tagctcatag atgatataat tgaagatacc agtagaatag aggtgtagta tgtcaatttc
    16861 ttataataaa ttgtggaagt tattgattga tttgaatatg aacaaaactc aacttcgtga
    16921 aatatctggc gtcagtccca atgtaatagc aaaactcggt aaaaatgagt tggttgcaat
    16981 ggagtcatta ttgaaaattg ctgctgcctt aaatgttgat gttagtgaaa taattagttc
    17041 agatgttata gatgcggagg atgttaaata aatggcaaaa aataaagttt taaacatcac
    17101 tgaaattgaa gatggtgtaa aaaaaatact ttcaaatagg ggcgatgatt tcatcactca
    17161 atttttgtcc ttgtacgata tccccaaaac gtcgattaca cgtgcaaaat caaaattcga
    17221 taatggtgaa ccatttgtga ttaagaacaa acttcattat gaagaaattg aaggcaacgt
    17281 tgtcattgcg attgatgcga ttgctcaatc aatcaaagag caaaaatcta aaccacgata
    17341 tatcttggta aacgattatt tagatattgc tgcaattgat acgaaaactc atgaaacttt
    17401 gaatattcca attgaagagc taccggctaa tgctgacttt ttcttagcgt ggaatggcat
    17461 tgagaaggct gattatcaag ctgaaaatcc agctgaccga aaagctgctg aacgttttgc
    17521 aaaattgtat gatgtggtgg ctaaagataa tcctgatgcc aacgagcatg catttaactt
    17581 gttcttgatt cgagtgctgt tcttgttgtt tgcagaagat acaggaatta tgaataaggg
    17641 ttcctttaca aatgttctaa aaacaagaac ggctgaagat ggttcgaatt ttaacgaagt
    17701 aatcaaagaa ctctttgcaa tcttggatat taatgagtta gctcgcaatg gcaaggatga
    17761 gtggctactt gaatttccat atgttaatgg aaaactgttt ggtgagccac atgtggattt
    17821 gaccttttca aaagtttcaa gagagctgct tattgaagca ggggaattat tgaattggaa
    17881 tgaaatcaat cctgatatcc ttggcgcaat gattcaatca gttgcttcag cagaagaccg
    17941 tcacgttgcc ggtatgcatt atacaagcgt gccgaatatc atgaaagtca tcaagccgtt
    18001 attcttagat gatttgacag agtcatttga aactttaaag cttcgcagtg aagaaaacga
    18061 gctgaaggac attactgaaa aaacaagaaa cgataataag agaacaatca tcaatgatct
    18121 aagtgctttg ttgacgcgaa tttctaatat taagtttttg gatcccgcca gtggttctgg
    18181 taattttctg atcattgctt ataaggaaat ccgtagactt gaaattaaga ttttgcaatt
    18241 gttgggcgac ttacaaaata atgattccat gccactatca gtgattcatc ttggtaattt
    18301 taatgggatt gaattggatg attttgcgca tgaagtagca agactttcat tatggattgc
    18361 tgaacatcaa atgaataaag agcttgaaga agcaatacca ggtaccattg ctgagttgtt
    18421 accgttaaaa gatgctggga atatagtgtg tgccaattct cttcgaattg attggaatga
    18481 ggtgttgcga ctcaaagaaa atcatgaaat atatataatg ggaaatccac cgtatctggg
    18541 caaaagtctt caaaatgctg aacaacgtga agatatggaa aaacttgttg gcgatattcg
    18601 aaaatataaa ttgcttgatt atatttttgg gtggttttgg aaagctaaac aatatattgc
    18661 aacatctcat ggctcgttcg catttgttac tactaattca atttgtcaag gtgaacaagt
    18721 tagtatcctt tggccgtacc ttttaaaaga tgaggtaaga atttcatttg catatcagag
    18781 ctttaagtgg aacaacaatg caaagaataa tgccggtgtg actgttgtca ttattggtat
    18841 gacaaataat gtgaatattc atcctaagtt atatttagag aatgggtcag taatgagtgt
    18901 ttcaaatatt aatgcatatt tagctaatgg tgaaaatgta tttgtcaacg aatcaagaaa
    18961 tagtatttca aatttaccct ctttgaaatt tggcagtatg gcaaatgatg gtggtaatct
    19021 agttttttca gtggcagaat actgg
//
LOCUS       DAYYAJ010000050        18775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000050 DAYYAJ010000000
VERSION     DAYYAJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.050"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..1526
                     /locus_tag="ACHATZ_001447"
     CDS             99..1526
                     /locus_tag="ACHATZ_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8403981.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1618..1983)
                     /locus_tag="ACHATZ_001448"
     CDS             complement(1618..1983)
                     /locus_tag="ACHATZ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8403982.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1980..2345)
                     /locus_tag="ACHATZ_001449"
     CDS             complement(1980..2345)
                     /locus_tag="ACHATZ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8403983.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2537..2785)
                     /locus_tag="ACHATZ_001450"
     CDS             complement(2537..2785)
                     /locus_tag="ACHATZ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403984.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2784..2912
                     /locus_tag="ACHATZ_001451"
     CDS             2784..2912
                     /locus_tag="ACHATZ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8403985.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2969..4663
                     /locus_tag="ACHATZ_001452"
     CDS             2969..4663
                     /locus_tag="ACHATZ_001452"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8403986.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4763..4936
                     /locus_tag="ACHATZ_001453"
     CDS             4763..4936
                     /locus_tag="ACHATZ_001453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8403987.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5314..6447
                     /locus_tag="ACHATZ_001454"
     CDS             5314..6447
                     /locus_tag="ACHATZ_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8403988.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6478..6798
                     /locus_tag="ACHATZ_001455"
     CDS             6478..6798
                     /locus_tag="ACHATZ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8403989.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6827..7291)
                     /locus_tag="ACHATZ_001456"
     CDS             complement(6827..7291)
                     /locus_tag="ACHATZ_001456"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8403990.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7423..8676)
                     /locus_tag="ACHATZ_001457"
     CDS             complement(7423..8676)
                     /locus_tag="ACHATZ_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8403991.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYYLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8705..9022)
                     /locus_tag="ACHATZ_001458"
     CDS             complement(8705..9022)
                     /locus_tag="ACHATZ_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8403992.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9048..9368)
                     /locus_tag="ACHATZ_001459"
     CDS             complement(9048..9368)
                     /locus_tag="ACHATZ_001459"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8403993.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9832..11247)
                     /locus_tag="ACHATZ_001460"
     CDS             complement(9832..11247)
                     /locus_tag="ACHATZ_001460"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8403994.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11264..12580)
                     /locus_tag="ACHATZ_001461"
     CDS             complement(11264..12580)
                     /locus_tag="ACHATZ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8403995.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12730..13428)
                     /locus_tag="ACHATZ_001462"
     CDS             complement(12730..13428)
                     /locus_tag="ACHATZ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8403996.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13717..14706)
                     /locus_tag="ACHATZ_001463"
     CDS             complement(13717..14706)
                     /locus_tag="ACHATZ_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8403997.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14786..15619)
                     /gene="msrA"
                     /locus_tag="ACHATZ_001464"
     CDS             complement(14786..15619)
                     /gene="msrA"
                     /locus_tag="ACHATZ_001464"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8403998.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15794..17428)
                     /locus_tag="ACHATZ_001465"
     CDS             complement(15794..17428)
                     /locus_tag="ACHATZ_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8403999.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17415..>18775)
                     /locus_tag="ACHATZ_001466"
     CDS             complement(17415..>18775)
                     /locus_tag="ACHATZ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404000.1"
                     /translation="YGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTII
                     PAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIK
                     NDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQ
                     ALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQK
                     TPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKE
                     NYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWE
                     KGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNA
                     GENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNE
                     NK"
BASE COUNT         5933 a         3702 c         3139 g         6001 t
ORIGIN      
        1 ttatatgctt tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc
       61 ccatattgtg caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga
      121 aaaatatttt aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc
      181 tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata
      241 acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca
      301 ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt
      361 cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat
      421 taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag
      481 cttattttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg
      541 aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc
      601 tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg
      661 gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc
      721 tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac
      781 gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata
      841 aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt
      901 cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc
      961 ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa
     1021 accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat
     1081 tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag
     1141 tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta
     1201 attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc
     1261 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
     1321 aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata
     1381 taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac
     1441 cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta
     1501 tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta
     1561 tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca
     1621 cttattttgg acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg
     1681 gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc
     1741 gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt
     1801 tttaatagca ttgttctcgc tgactcggtc gctttgcact tttgtatgga caaataaata
     1861 tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat
     1921 atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt
     1981 catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa
     2041 gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc
     2101 ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa
     2161 cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg
     2221 acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg
     2281 atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga
     2341 aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt
     2401 taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt
     2461 ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag
     2521 caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt
     2581 tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac
     2641 ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt
     2701 tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa
     2761 gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag
     2821 gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca
     2881 gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac
     2941 atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc
     3001 cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga
     3061 tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac
     3121 tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc
     3181 tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg
     3241 aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc
     3301 aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg
     3361 cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc
     3421 tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc
     3481 acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg
     3541 tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat
     3601 cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc
     3661 tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga
     3721 ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac
     3781 caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt
     3841 cactaatggg tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga
     3901 aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa
     3961 cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac
     4021 cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc
     4081 aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga
     4141 tgtcaatatg tctattgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga
     4201 aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca
     4261 gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt
     4321 agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa
     4381 taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga
     4441 tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga
     4501 gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc
     4561 tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga
     4621 tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa
     4681 agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat
     4741 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga
     4801 aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc ttatgttttt
     4861 gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4921 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
     4981 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
     5041 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
     5101 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
     5161 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
     5221 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
     5281 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
     5341 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
     5401 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
     5461 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
     5521 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
     5581 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
     5641 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
     5701 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
     5761 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
     5821 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
     5881 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     5941 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     6001 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     6061 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     6121 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     6181 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     6241 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     6301 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     6361 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     6421 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     6481 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     6541 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     6601 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     6661 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     6721 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     6781 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     6841 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     6901 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     6961 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     7021 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     7081 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     7141 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     7201 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     7261 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     7321 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     7381 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat ctaaagcttc
     7441 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
     7501 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
     7561 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
     7621 agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat
     7681 gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat tgaagacacc
     7741 tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc ctactaaata
     7801 aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa agctgtcaaa
     7861 gaaaggtttg ttcacgataa acggtgcgct atgtccagct gcgattgcat taacgtttgc
     7921 ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg ctccgtgtaa
     7981 accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg gataagagtt
     8041 tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga tatcatcaac
     8101 accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga tcattgctgg
     8161 tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca ttttgatgat
     8221 caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc caacaataat
     8281 agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga agaaagagcc
     8341 taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta aataataagc
     8401 aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag cagcagcccc
     8461 gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga acccgggaat
     8521 cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca tactcaccat
     8581 aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg ctacaggtat
     8641 aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt ttctttactg
     8701 tagattacgc gttcaatcga cgatacagat caatgatctc ttttgctaaa tctgtaaaag
     8761 ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat
     8821 ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg actaaagatt
     8881 cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc gcagcagcaa
     8941 tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc atgatggctt
     9001 ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta tttgtttatt
     9061 agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc agccatattg
     9121 attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt ttgcataaag
     9181 cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg attatccgca
     9241 tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc tgccttttgc
     9301 atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat gattgttttc
     9361 ttttccattt taaacacgtt cctttctata cttacacttg tatataagca agtagcgtgc
     9421 caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat accaagttgc
     9481 atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat tacctttttt
     9541 cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt ttcaaataaa
     9601 cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cataagatgt
     9661 tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca acctctcaat
     9721 aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt cacaacctct
     9781 tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta tttatttata
     9841 gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc acgaagcaga
     9901 ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc catatcgatt
     9961 cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat agccttcgca
    10021 atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat
    10081 acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat attctcttcc
    10141 cataccgttt tctgttatca gccaagggat attatgataa ttttctttta gatacatggc
    10201 tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca ttttcttccc
    10261 aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt cattccattg
    10321 ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga aatctactgt
    10381 atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg attgtttttg
    10441 acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa aaaatactcg
    10501 attataaaac aaatcataca tataagcagc tttttggtct tctggagaat tggatcgagc
    10561 atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc cgctgcggta
    10621 aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca tccacttttt
    10681 tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt ggggaacgat
    10741 tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa aggcaatttg
    10801 agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat atttttccaa
    10861 aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt ctgcttcgat
    10921 caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta tacagttctc
    10981 ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca gatggatgga
    11041 tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta cggctgggcc
    11101 atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa acgaatcctg
    11161 tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag caccaagaat
    11221 aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc ccatcatttt
    11281 ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca ataatacaaa
    11341 caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca ttgacgatcg
    11401 gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa gcggttgcaa
    11461 aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct aaaattggat
    11521 tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc ccaggagcaa
    11581 gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat actgcaatca
    11641 gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa aatggcatag
    11701 taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca gcaattgccg
    11761 ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata ccaatcaccc
    11821 aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt acgacacctt
    11881 ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct gtaagttctt
    11941 tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg aataaagcag
    12001 aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt agtttcttct
    12061 gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt cccaaaaata
    12121 accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca gtctgatttt
    12181 cattcactaa ttcataaaca gttggattaa cagataaata agacatcact gcgatgatac
    12241 cagggaatac accatccact ttattcagct tggctaattc gatccctatc agaaaaacag
    12301 catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata ggctttaatt
    12361 cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca aagaccattg
    12421 ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt
    12481 tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc cctaaaacat
    12541 tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct tctccttgac
    12601 gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa ctatttactc
    12661 ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata aaatacatca
    12721 gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc tccgatttat
    12781 tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt cgatggcctg
    12841 aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat cataattttt
    12901 attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg ctacgtttcc
    12961 gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc
    13021 ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga tttgattctt
    13081 cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc tcagttcaaa
    13141 taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc
    13201 aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa ggatattcct
    13261 ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta aataatcgga
    13321 caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct ttaaatatct
    13381 aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac cacatacctc
    13441 cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat ctgatgattt
    13501 tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga tatttaccat
    13561 aaaaaagact aggataaaat tcactcctag ccttcgatca tttttatttt gaacgacaaa
    13621 aaatatgatg ttcaataaca gacgatcttc attcgattct tgatgtcatc ttttgttttt
    13681 tttaatatat ggataaaaag tttgattaca tcgtttctat tctagaattt ggtcgattct
    13741 caattcttct tctggtgaga aatctaaatt atccatcatt cgaacacttt cttgtaactg
    13801 acttaaccga cttgctccga ccagtacact tgtaacagat ttttgtcgca aattccatgc
    13861 aagggccatt tgcgctaatg attgtcctcg tgatacggcg attgtttgca gtgctttgat
    13921 tttttccaga gttgatccaa cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc
    13981 tctagaatct tcaggaatac catgtagata acgatttgtc agcaaccctt gagcaagcgg
    14041 actgaacgtg attgccccta gcccttcttc ttctaatact ggagatagat cgtcttctag
    14101 tagtgcacga gaaaacatat tgtagcgcat ctgatgaata atgaacggtg cttcctttct
    14161 ttttaaaatt tcagtcattt tttttgtatc ttctccatta tagttggata taccaatata
    14221 taatgctttt ccttgtctta caagttgcat caaagcttct gctgtttctt ccaaaggtgt
    14281 atcagggtct gggcgatgat gatagaagat gtcaacataa tctagcccca ttctttggag
    14341 actttggtca caacttgaaa tgatggattt acgagagccc cattccccat atggcccttc
    14401 ccacatatga taacctgctt tgctagaaat aatcagttca tcacggtagg cttgaaaatc
    14461 ttctctaaaa atacgcccaa agtttttctc tgcagctcca gcagggcctc cataattatt
    14521 cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt
    14581 atggatagga tcataatcac caaaattttg ccataatccg agagataaaa tcggtaattt
    14641 caaacctgaa tgaccggatt tacgatattc cattttttca taacgtgcac tatctgcaat
    14701 atacaatttt tctcctcctc atcaaatctt tttaaaacgc tttctttttt ctatttttag
    14761 tttactacat tttaaagaaa aggagttaat caaaatgatt ttttcgtata tttttttgcc
    14821 atttgttttt tactcgttga tagatcagaa attgattacg tgcttgctgg atttttttat
    14881 aacgtttcgg ttgttttttg taaaattgct gatgatagtt ttctgctggc caaaacgtac
    14941 cggctggttc gatttttgtg acgattggtt ggtggaaagt tcctgaactg tctagtttct
    15001 gttttgattg ttccgctaat tctttttgtc gttcgacttg ataaaaaatc actggacgat
    15061 attgcttccc tctatcttga aattgtccaa atgtatctgt gggatcagta atttgccaat
    15121 agattgtcag caactcgcta taacttattt ctcttgtgtc ataaatgatc tccacagctt
    15181 ccacatgacc tgtgtaacca ccacttactt gatcataatt tggtttttca acatgtccgc
    15241 ccgtgtatcc tgacaaaaca gataggatcc cttttttcga ttcaaacggc tcgaccatac
    15301 accagaaaca tccccctgca aatactgcac gttcttgata gtcagcatct tgcatggcat
    15361 gcttggaaaa gtcatatttt tgctccttat caaatccttc cgtaatcaat aaataaaagt
    15421 ccattacatc aggcgtcaga ttcattcgga tagctaatgg acgtagtgtt gcttctagtt
    15481 ttgataactg ttctttaacg gatatatttt ctcttatgtc attctttgtc tgaatcaata
    15541 cttttcgttc ccaatctcgt gtattcggat tcaaaatcag attataaagt gtttgaatga
    15601 atatttgttc ctcatccata gattctcctc ctttccgaag aagacaagct agtgacttta
    15661 gctgtaattt atcataaagt gaggaaataa gcgattactt catcccccta cagatttttc
    15721 gtataaaaaa gagagaatcc ttatcgacag attctccttt ttaatcaatt tattttataa
    15781 atgaaaagtt aatttacctt ttatggaaaa ctattttaaa aaataaacat aaatatgcag
    15841 ttaatgtaac aagataagaa ataatagtcg ttactgtata acttgtgctt actttaatat
    15901 cagcaactcc tttgactccg cttatttgta ccaatccatt agcattcacc tgctccttta
    15961 caggttgatg atttatttct acattcgttc ctaaataagc atatacagga agtgtgatta
    16021 cttggctctt ctcattagtt actctatata tgatagaact accagttgtt ttataaggaa
    16081 ggtttaccca ttgactgcca atctttgctt cgtgttttat tatatgctcc ttattctcca
    16141 atgaagcgat aggagaataa tcatataaaa aataatccat tcctttagtg gtatacgcct
    16201 ctactgtttc tttgtttaca acaatatgtc ccggctgaga aattattttt tgattgaaat
    16261 tttttttact agaaacagtt aaagtgatta ataacaaaac agtcataaat aaataaaata
    16321 gttctcttgt cttattgtat cttctatttt tgaaaaatat catcgttaat atcagagaag
    16381 agaatattat ttgcaagctt aagatccgcc aaggaaattg taacaatttg gctggcgtat
    16441 tttgcagaag ataccatggc accaaactag ttgtagctag ccaagtcaaa cacgtaccat
    16501 aataaatctt tttttctatg tttgttaatt ttttaaataa aatcagggga atacataaag
    16561 ataacagacc aactagtccc attgagtaag tagttaagtc gttgttcata gcttcagtca
    16621 gactatcgaa cacattagat gcatgagcat ataaatttgt cacgctaggt ttgttgattt
    16681 tttggtagag catttgttca aacatcggat accaaaaagc actcgtcaga accaatgtca
    16741 ttcctgccgc actaaataat tttttaaata tagcaataaa atctgcattc ttgaaacctt
    16801 tagaaaccaa acgaataaaa ataaaaatga taattaatgc tgtactcatt aatgttgata
    16861 gtacatggct gtacactagc aaactcatac tcaatgctag ctttacagac ggatactttt
    16921 cttcttttcc agagatgatt tgataagcat aaaataatac agggataaca agtgttattg
    16981 cgatagcttc ggcaattgcg gaacgatagt agatgtctac tgtccgataa gcagagaaaa
    17041 tatagagaca actaaataaa aatgccgctt ttttttgttt taaaaaccgc tcgccataat
    17101 gataagctac gagtgttgta cacaagttca ataaccaaac gtaaataata tagcttacaa
    17161 tcaaattttt tgtcatccaa tataacatta cagctggaaa aaaagtcaga aagggataaa
    17221 aataatttac accatatcca gtataattga atgtctcata attaatcgga ctggtaaaaa
    17281 tagtcgataa ccccttgatt cgagataagt gaaaagaaag atctccagtg gtataaatac
    17341 tctgtgtatt tttgaaaaag atgaaagaat atatactact aactataatt aatccactca
    17401 atattactac ttttttattt attttcattc ttcttgttta tctccttttt agtcacatat
    17461 cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac ttgaatacac cactaaacaa
    17521 taagtcagaa tactgatcca taaagccatg agagcagttg gagtatattt tgttgtaatt
    17581 tctatcttat tttctccagc atttacatcc acattcacta agccatgttt tttataagag
    17641 tcatttacga ctttaccgtt tacttttacc tgtgtaccta aataagcata tactggtact
    17701 gtcacttttt gctttttgtt gcttgccact gtgtaagtaa tgttattatc tgcagcttta
    17761 tacttccctt tttcccacga ttctcctact tttacttcat gtttttctaa gtgaggcctt
    17821 tcttttatcg cttctgttgg ggcgtaatcg tataatcctc ccatatctga tgttgtataa
    17881 cgtgggagtg tttcttcatt gataacaata tgaccgtgtt ttgattggat aacttttgcg
    17941 taattttctt ttgctgctat agacgtgacg agtagaaaca ccacagtagc gccaatgatt
    18001 aaccatgaat attttttatt catagtcttt ttcataaaaa caagcgttat gatcattgaa
    18061 gaaaaaaaga tttggatacc taagatacgc caaggaaact ggagtaagct tatcggtgtt
    18121 ttttgaaaca aaaaccatgg aaataaatta gttacgcata cccatgtaga aactacgcca
    18181 acatagatga ttttttcttt tttctccatt tctttcagta tgaataaagg aaaaatcaac
    18241 gcaaccaatc ctaataaccc catagtgtat gttgtaatat cgttatttaa cgcttgaatg
    18301 atactatctg aaatagaaag tgcttctagc tgcaaatctg ttttgaaggg actgtgtatt
    18361 tcttgataat tcatctgctg caacatagga taccaaaaat agctagttaa tgcacaagtc
    18421 aaaaaaacag cctttactaa attcacgacc acaaatttta tatcgttttt tatttgcttg
    18481 cttaaaaaca aacgaactat aatcatacaa gcaatcaatg cactacacaa tagtgtagtt
    18541 aagacatgag tatacacaag tagacttata ctgacagata aagcgatgac ttttttttct
    18601 ctattgaata tcaatccata agcataatat aaaacagctg ggataatcgt aagagcgatt
    18661 gcttctgcaa ttgcggatcg ataatagata tcaactgttc gataagttgc aaaagtatat
    18721 aaacaactaa ataaaaaagc agcatctatc tttttaaaaa acttttctcc ataat
//
LOCUS       DAYYAJ010000051        18518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000051 DAYYAJ010000000
VERSION     DAYYAJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.051"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..482)
                     /gene="yajC"
                     /locus_tag="ACHATZ_001467"
     CDS             complement(102..482)
                     /gene="yajC"
                     /locus_tag="ACHATZ_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8404001.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(579..1724)
                     /gene="tgt"
                     /locus_tag="ACHATZ_001468"
     CDS             complement(579..1724)
                     /gene="tgt"
                     /locus_tag="ACHATZ_001468"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8404002.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1942..2640
                     /locus_tag="ACHATZ_001469"
     CDS             1942..2640
                     /locus_tag="ACHATZ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8404003.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2808..5447)
                     /locus_tag="ACHATZ_001470"
     CDS             complement(2808..5447)
                     /locus_tag="ACHATZ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8404004.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6066..7097)
                     /gene="queA"
                     /locus_tag="ACHATZ_001471"
     CDS             complement(6066..7097)
                     /gene="queA"
                     /locus_tag="ACHATZ_001471"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8404005.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7290..8465
                     /locus_tag="ACHATZ_001472"
     CDS             7290..8465
                     /locus_tag="ACHATZ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8404006.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8469..9110
                     /locus_tag="ACHATZ_001473"
                     /pseudo
     CDS             8469..9110
                     /locus_tag="ACHATZ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9107..10024
                     /locus_tag="ACHATZ_001474"
     CDS             9107..10024
                     /locus_tag="ACHATZ_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8404007.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10021..10692
                     /locus_tag="ACHATZ_001475"
     CDS             10021..10692
                     /locus_tag="ACHATZ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404008.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11233..11721)
                     /locus_tag="ACHATZ_001476"
     CDS             complement(11233..11721)
                     /locus_tag="ACHATZ_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8404009.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11922..12188)
                     /locus_tag="ACHATZ_001477"
     CDS             complement(11922..12188)
                     /locus_tag="ACHATZ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8404010.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12188..12451)
                     /locus_tag="ACHATZ_001478"
     CDS             complement(12188..12451)
                     /locus_tag="ACHATZ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8404011.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12972..13112
                     /locus_tag="ACHATZ_001479"
                     /pseudo
     CDS             12972..13112
                     /locus_tag="ACHATZ_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13254..15077)
                     /locus_tag="ACHATZ_001480"
     CDS             complement(13254..15077)
                     /locus_tag="ACHATZ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8404012.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15675..18380)
                     /locus_tag="ACHATZ_001481"
     CDS             complement(15675..18380)
                     /locus_tag="ACHATZ_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8404013.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         5276 a         3925 c         3472 g         5845 t
ORIGIN      
        1 attgagcaat atatcccatg ctcttatctc tttctttttt gaataaaaaa tgaggtagga
       61 catctttctg ccctacctcg tctttaaata agctttgata cttatttttc ttccttctct
      121 tctgtctctt ccagcgtttc tgttgtttca gtttttactt caactgtctc agctggttct
      181 tctgttgttt caacagtagt cgttgcatca tttgttacga cagtccctgg tttgaccgtt
      241 ttgattgctg cacgatcgta ctcaaggaag atcccttcgc agtctaaaac aactgtccgt
      301 ttgtcattgt cgatttctga aacaactcca tgtaatccgc cgatcgttac gactgaatca
      361 cctggtttca tgctatctaa caagttttga cgttcttgct gttgtttctt ttgcgaacgt
      421 gacataaaaa accacattgc taatagcgcg ataaacatca caagcattgg taatgttccc
      481 atttttttca cctcactaat tctgtatcta caaaaaacac tttagcagaa aaatgctgat
      541 aacactagtt ttttacttaa tttttaaaag attttccttt agaagctttt tgcgttttct
      601 ttattgaacc cgtattcttc aaaaaaggct tctctaaatt caagcaaatt atcatccatg
      661 attgcttgac gtacttgttt cattagattc aacaagaagt ataaattgtg gtaagaagtc
      721 aaacgaatcc caaatgtttc atcacatttg atcaaatggc ggatataggc acgtgtatag
      781 tttttacatg tataacaatc acatttctca tcaattggac ggaaatcatg ggcatatttc
      841 gcatttttca ctactagacg gccttgagaa gtcatacatg tgccatttcg agcgattcga
      901 gtaggcaaga cacagtcaaa catgtctact ccacggataa caccatcgat caatgaatca
      961 gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat
     1021 tctaatacac gattcatttc tgatttaggt tccccgacag ataacccgcc gattgagtaa
     1081 ccagggaaat ccatcccgac tagatctttc gcgctttgac gacgaagatc ttcaaaccct
     1141 gctccttgga tgattccaaa taatccttga cggtctggat tagcatgagc tttcaatcct
     1201 cgttctgccc atcgagacgt acgttctact gattttttca cgtagtcgta actttcatcg
     1261 aatggtggac attcgtcaaa actcatcatg atatcagagc ccaatttatt ttggatgttg
     1321 attgcttttt ctggtgataa aaacatacgc gaaccgttca agtgattttt gaaatgcacg
     1381 ccttcttctt cgatattccg catatcactt aatgaaaaaa cttgaaagcc gcctgaatct
     1441 gttaaaatcg gttgatccca gttcataaat ttatgcagtc cgccagcttc ttctactaag
     1501 tcttcaccag ggcgtagcca taaatgatat gtattactta aaataacgcc agcacccatt
     1561 tctttcaact cttctgggga cattgttttt actgtagcta atgttcctac tggcatgaac
     1621 attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt
     1681 ttttctttct tgattaaacg atagcgaatg gcaggttgcg tcattcattt ccctactttc
     1741 tatataaaat atcttgacaa ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt
     1801 ccctcggctt ccttctactt tatttaatat tctttttttt atcctttttt tatgaataac
     1861 aaaaaaaaga aaaacattat gacagaaata ttttttgata catggtatat tggtgttatt
     1921 aaagaaaagg agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag
     1981 agtctaaaag gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt
     2041 attgctacta ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat
     2101 ggatcagcga gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc
     2161 ggaattattt ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt
     2221 gatattttac gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc
     2281 aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca
     2341 ttatggggtt tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc
     2401 tattttattt actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg
     2461 atcactgcaa gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt
     2521 tcatttatcg gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc
     2581 agcccataca tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa
     2641 aagagagtgt gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt
     2701 aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc
     2761 tttttgtata gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct
     2821 tctgcttccg gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat
     2881 aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc
     2941 gtaaatggca gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc
     3001 aaagctttat tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac
     3061 acatgcagaa tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga
     3121 cttgcgtcta cccatgggct gatagtatcg acataactac ctaaataata accaaacaag
     3181 gttacgatgg tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag
     3241 atcccttcat ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt
     3301 cctaaagcaa atcctggaag tccatcagca actacattga caaacagtag ttgcacagct
     3361 gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata
     3421 ttacagctta gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga
     3481 ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt
     3541 tgcgccacat ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct
     3601 ggtgcatcat tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat
     3661 ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca
     3721 cgcgcatcta attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac
     3781 ggatcattca agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca
     3841 gtgatcatga ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct
     3901 ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct
     3961 tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc
     4021 agtgcccgtt taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta
     4081 aagcgcggag caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt
     4141 tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga
     4201 tatttttctt ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct
     4261 acttctgttg gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct
     4321 gtacatagtg cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata
     4381 ttcgttactt catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc
     4441 gttcctgttt tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac
     4501 tgtcggatga tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta
     4561 acaataacag tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac
     4621 atctcaagga gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct
     4681 aaagcgatca tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt
     4741 tgcatagtat tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg
     4801 gcagtgacga tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta
     4861 agttggtcac ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct
     4921 tcactttctc cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg
     4981 gcatcggcag aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc
     5041 tctgactcaa cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata
     5101 tcttgcaagg attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag
     5161 ttgatcacaa cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca
     5221 aatgcggtat aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac
     5281 aagcttcgaa agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat
     5341 ccctgctttt ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct
     5401 tctgttacct tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt
     5461 taaaatcttc gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt
     5521 caaataaagg gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt
     5581 gcaaacgcta ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag
     5641 acagaagaat ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc
     5701 acccagacat gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat
     5761 cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct
     5821 gaaaattgaa taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt
     5881 ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca
     5941 tttctgttta tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct
     6001 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
     6061 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
     6121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
     6181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
     6241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
     6301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
     6361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
     6421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
     6481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
     6541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
     6601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
     6661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
     6721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
     6781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
     6841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
     6901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
     6961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     7021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     7081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     7141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     7201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     7261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     7321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     7381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     7441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     7501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     7561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     7621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     7681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     7741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     7801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     7861 ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg
     7921 aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt
     7981 tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag
     8041 tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag
     8101 caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg
     8161 tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg
     8221 tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata
     8281 ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca
     8341 aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg
     8401 gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg
     8461 tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg
     8521 aacatcttta tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg
     8581 gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct
     8641 tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta
     8701 aattacctgc gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt
     8761 acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga
     8821 ctgagttcta atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg
     8881 gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg
     8941 ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt
     9001 tggcaggtac gatccctgta acaattttag cactattggc tgattggctt ctaggtctct
     9061 tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact
     9121 aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct
     9181 tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca
     9241 gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat
     9301 catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc
     9361 aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga
     9421 aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca
     9481 aacgtggttc ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac
     9541 cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt
     9601 agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga
     9661 aacttatggc ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc
     9721 agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag
     9781 ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc
     9841 agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt
     9901 aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct
     9961 agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa
    10021 gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat
    10081 ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc
    10141 tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca
    10201 aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc
    10261 attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat
    10321 tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc
    10381 cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta
    10441 ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg
    10501 ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga
    10561 accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg
    10621 gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt
    10681 acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa
    10741 ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg
    10801 ggcaaagagt tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc
    10861 aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc
    10921 caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc
    10981 tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc
    11041 cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg
    11101 ccgtatcggt aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg
    11161 ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc
    11221 ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt
    11281 caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt
    11341 ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga
    11401 aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt
    11461 ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc
    11521 aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat
    11581 tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc
    11641 ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat
    11701 tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc
    11761 cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa
    11821 tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga
    11881 gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag
    11941 gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt
    12001 ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc
    12061 gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa
    12121 ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag
    12181 ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt
    12241 tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa
    12301 tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa
    12361 tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag
    12421 cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta
    12481 tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt
    12541 ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca
    12601 cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca
    12661 tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag
    12721 gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag
    12781 atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg
    12841 attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg
    12901 ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac
    12961 ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg
    13021 aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc
    13081 aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc
    13141 tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga caaaacagac
    13201 cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc
    13261 ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt
    13321 aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga
    13381 ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca
    13441 taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca
    13501 tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct
    13561 agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg
    13621 atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt
    13681 agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc
    13741 ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac
    13801 cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca
    13861 atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga
    13921 gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag
    13981 ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa
    14041 tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga
    14101 ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc
    14161 gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt
    14221 tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg
    14281 atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt
    14341 tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa
    14401 gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc
    14461 gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca
    14521 acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat
    14581 gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa
    14641 atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct
    14701 gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag
    14761 ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa
    14821 gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg
    14881 gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt
    14941 tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca
    15001 aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac
    15061 ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag
    15121 tcgaaaattt atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa
    15181 ctttaagaaa ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa
    15241 tacagcaaaa atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat
    15301 agaacttgac ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag
    15361 atgtaaggct gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa
    15421 cctcttgata aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa
    15481 aacataagaa gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta
    15541 caacctcttg gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc
    15601 aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt
    15661 cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc
    15721 attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa
    15781 ccgatcatga tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc
    15841 cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc
    15901 cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata
    15961 atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg
    16021 aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact
    16081 cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa
    16141 aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt
    16201 cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat
    16261 agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg
    16321 gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat
    16381 aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct
    16441 ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc
    16501 acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca
    16561 ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga
    16621 atgatcggta attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta
    16681 tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt
    16741 tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg
    16801 agaagcgaat caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc
    16861 gtatcgatga cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata
    16921 ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag caattcatca
    16981 atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga
    17041 ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt
    17101 ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt
    17161 tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca
    17221 gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt
    17281 gccacttgtc caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca
    17341 tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct
    17401 aaatttttca catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt
    17461 gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct
    17521 gctagttgct taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt
    17581 tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc
    17641 tcatctactt tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt
    17701 tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa
    17761 ttttcatctg ttgataaaat catatcttta actttattga tacttactag gttcgtatcg
    17821 atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat
    17881 tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc
    17941 gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta
    18001 aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg
    18061 gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta
    18121 tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct
    18181 gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc
    18241 cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc
    18301 agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc
    18361 gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac
    18421 catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat
    18481 cacggcaatt gtctcattgt aatctcttac atattgag
//
LOCUS       DAYYAJ010000052        18514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000052 DAYYAJ010000000
VERSION     DAYYAJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.052"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..884)
                     /locus_tag="ACHATZ_001482"
     CDS             complement(60..884)
                     /locus_tag="ACHATZ_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8404014.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(857..1537)
                     /locus_tag="ACHATZ_001483"
     CDS             complement(857..1537)
                     /locus_tag="ACHATZ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8404015.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1560..2498)
                     /locus_tag="ACHATZ_001484"
     CDS             complement(1560..2498)
                     /locus_tag="ACHATZ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8404016.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2743..3594)
                     /gene="ispE"
                     /locus_tag="ACHATZ_001485"
     CDS             complement(2743..3594)
                     /gene="ispE"
                     /locus_tag="ACHATZ_001485"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8404017.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3748..6321)
                     /gene="mprF"
                     /locus_tag="ACHATZ_001486"
     CDS             complement(3748..6321)
                     /gene="mprF"
                     /locus_tag="ACHATZ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8404018.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6462..7268
                     /gene="proC"
                     /locus_tag="ACHATZ_001487"
     CDS             6462..7268
                     /gene="proC"
                     /locus_tag="ACHATZ_001487"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8404019.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7347..8903)
                     /gene="rny"
                     /locus_tag="ACHATZ_001488"
     CDS             complement(7347..8903)
                     /gene="rny"
                     /locus_tag="ACHATZ_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8404020.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9314..10363)
                     /gene="recA"
                     /locus_tag="ACHATZ_001489"
     CDS             complement(9314..10363)
                     /gene="recA"
                     /locus_tag="ACHATZ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8404021.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10472..11713)
                     /locus_tag="ACHATZ_001490"
     CDS             complement(10472..11713)
                     /locus_tag="ACHATZ_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8404022.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11953..12531)
                     /gene="pgsA"
                     /locus_tag="ACHATZ_001491"
     CDS             complement(11953..12531)
                     /gene="pgsA"
                     /locus_tag="ACHATZ_001491"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8404023.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12740..13663)
                     /locus_tag="ACHATZ_001492"
     CDS             complement(12740..13663)
                     /locus_tag="ACHATZ_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8404024.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14402..15688)
                     /locus_tag="ACHATZ_001493"
     CDS             complement(14402..15688)
                     /locus_tag="ACHATZ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8404025.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15681..16949)
                     /locus_tag="ACHATZ_001494"
     CDS             complement(15681..16949)
                     /locus_tag="ACHATZ_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8404026.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17201..17662
                     /gene="mscL"
                     /locus_tag="ACHATZ_001495"
     CDS             17201..17662
                     /gene="mscL"
                     /locus_tag="ACHATZ_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8404027.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17721..18167)
                     /locus_tag="ACHATZ_001496"
     CDS             complement(17721..18167)
                     /locus_tag="ACHATZ_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404028.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18260..18505)
                     /locus_tag="ACHATZ_001497"
     CDS             complement(18260..18505)
                     /locus_tag="ACHATZ_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8404029.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5288 a         4043 c         3235 g         5948 t
ORIGIN      
        1 aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt
       61 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
      121 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
      181 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
      241 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
      301 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
      361 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
      421 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
      481 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
      541 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
      601 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
      661 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
      721 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
      781 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
      841 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
      901 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
      961 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
     1021 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
     1081 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
     1141 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
     1201 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
     1261 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
     1321 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
     1381 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
     1441 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
     1501 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
     1561 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
     1621 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
     1681 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
     1741 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
     1801 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
     1861 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
     1921 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
     1981 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
     2041 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
     2101 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
     2161 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
     2221 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
     2281 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
     2341 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
     2401 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
     2461 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
     2521 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
     2581 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
     2641 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
     2701 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
     2761 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
     2821 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
     2881 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
     2941 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
     3001 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
     3061 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
     3121 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
     3181 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
     3241 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
     3301 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
     3361 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
     3421 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
     3481 tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
     3541 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
     3601 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
     3661 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
     3721 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
     3781 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
     3841 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
     3901 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
     3961 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
     4021 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
     4081 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
     4141 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
     4201 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
     4261 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
     4321 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
     4381 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
     4441 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
     4501 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
     4561 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
     4621 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
     4681 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
     4741 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
     4801 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
     4861 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
     4921 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
     4981 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
     5041 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
     5101 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
     5161 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
     5221 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
     5281 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
     5341 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
     5401 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
     5461 taatagccaa actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat
     5521 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
     5581 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
     5641 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
     5701 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
     5761 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
     5821 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
     5881 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
     5941 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
     6001 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
     6061 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
     6121 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
     6181 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
     6241 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
     6301 caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc
     6361 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
     6421 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
     6481 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
     6541 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
     6601 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
     6661 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
     6721 aagtcttatt catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg
     6781 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
     6841 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
     6901 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
     6961 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
     7021 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
     7081 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
     7141 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
     7201 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
     7261 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
     7321 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
     7381 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
     7441 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
     7501 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
     7561 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
     7621 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
     7681 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
     7741 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
     7801 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
     7861 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
     7921 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
     7981 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
     8041 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
     8101 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
     8161 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
     8221 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
     8281 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
     8341 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
     8401 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
     8461 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
     8521 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
     8581 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
     8641 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
     8701 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
     8761 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
     8821 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
     8881 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
     8941 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
     9001 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
     9061 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
     9121 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
     9181 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
     9241 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
     9301 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
     9361 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
     9421 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
     9481 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
     9541 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
     9601 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
     9661 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
     9721 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
     9781 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
     9841 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
     9901 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
     9961 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
    10021 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    10081 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    10141 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    10201 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    10261 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    10321 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    10381 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    10441 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    10501 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    10561 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    10621 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    10681 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    10741 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    10801 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    10861 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    10921 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    10981 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    11041 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    11101 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    11161 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    11221 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    11281 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    11341 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    11401 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    11461 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    11521 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    11581 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    11641 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    11701 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    11761 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    11821 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    11881 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    11941 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    12001 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    12061 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    12121 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    12181 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    12241 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    12301 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    12361 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    12421 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    12481 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    12541 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    12601 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    12661 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    12721 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    12781 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    12841 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    12901 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    12961 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    13021 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    13081 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    13141 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    13201 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    13261 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    13321 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    13381 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    13441 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    13501 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    13561 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    13621 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    13681 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    13741 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    13801 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    13861 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    13921 tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    13981 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa
    14041 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    14101 ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt
    14161 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    14221 ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt
    14281 tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg
    14341 gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg
    14401 tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag
    14461 ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc
    14521 gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt
    14581 ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg
    14641 gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt
    14701 ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa
    14761 agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta
    14821 aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt
    14881 gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt
    14941 ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt
    15001 gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag
    15061 gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc
    15121 acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga
    15181 tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag
    15241 ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt
    15301 cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct
    15361 gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag
    15421 ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt
    15481 ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa
    15541 attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat
    15601 ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat
    15661 tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc
    15721 tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt
    15781 ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct
    15841 tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt
    15901 gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga
    15961 atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat
    16021 cctcggaacg tatccacgct actagaggca taataagcta gactttcttt ctctctgaca
    16081 ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca
    16141 aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga
    16201 acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac
    16261 agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct
    16321 ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct
    16381 tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac
    16441 cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca
    16501 aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag
    16561 tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt
    16621 aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa
    16681 ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt
    16741 gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta
    16801 tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc
    16861 gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga
    16921 ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc
    16981 cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat
    17041 aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat
    17101 gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt
    17161 tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga
    17221 gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt
    17281 tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt
    17341 cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc
    17401 gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat
    17461 cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc
    17521 caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat
    17581 acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa
    17641 tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc
    17701 ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga
    17761 atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct
    17821 tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg
    17881 ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca
    17941 aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc
    18001 ttcccatcag tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct
    18061 tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa
    18121 aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc
    18181 cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa
    18241 tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag
    18301 taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat
    18361 caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct
    18421 ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct
    18481 tgattgaagc taaagttgtt ggcatacagt tcac
//
LOCUS       DAYYAJ010000053        18494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000053 DAYYAJ010000000
VERSION     DAYYAJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.053"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHATZ_001498"
     CDS             99..746
                     /locus_tag="ACHATZ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8404030.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHATZ_001499"
     CDS             1139..3784
                     /locus_tag="ACHATZ_001499"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8404031.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHATZ_001500"
     CDS             4094..5407
                     /locus_tag="ACHATZ_001500"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8404032.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHATZ_001501"
     CDS             5397..6050
                     /locus_tag="ACHATZ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8404033.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHATZ_001502"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHATZ_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8404034.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHATZ_001503"
     CDS             6947..7147
                     /locus_tag="ACHATZ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8404035.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHATZ_001504"
     CDS             complement(7401..9080)
                     /locus_tag="ACHATZ_001504"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8404036.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHATZ_001505"
     CDS             complement(9082..9294)
                     /locus_tag="ACHATZ_001505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8404037.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHATZ_001506"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHATZ_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8404038.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHATZ_001507"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHATZ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8404039.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHATZ_001508"
     CDS             13269..13466
                     /locus_tag="ACHATZ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404040.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACHATZ_001509"
     CDS             13619..14104
                     /locus_tag="ACHATZ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8404041.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHATZ_001510"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHATZ_001510"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8404042.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACHATZ_001511"
     CDS             14981..15508
                     /locus_tag="ACHATZ_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404043.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACHATZ_001512"
     CDS             15599..16336
                     /locus_tag="ACHATZ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8404044.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACHATZ_001513"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACHATZ_001513"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8404045.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACHATZ_001514"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACHATZ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8404046.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5980 a         3200 c         4022 g         5292 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa tttt
//
LOCUS       DAYYAJ010000054        18488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000054 DAYYAJ010000000
VERSION     DAYYAJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.054"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..738)
                     /locus_tag="ACHATZ_001515"
     CDS             complement(76..738)
                     /locus_tag="ACHATZ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8404047.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            922..2568
                     /locus_tag="ACHATZ_001516"
     CDS             922..2568
                     /locus_tag="ACHATZ_001516"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8404048.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2559..3641
                     /gene="uxuA"
                     /locus_tag="ACHATZ_001517"
     CDS             2559..3641
                     /gene="uxuA"
                     /locus_tag="ACHATZ_001517"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8404049.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3749..5302)
                     /locus_tag="ACHATZ_001518"
                     /pseudo
     CDS             complement(3749..5302)
                     /locus_tag="ACHATZ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5450..6591
                     /locus_tag="ACHATZ_001519"
                     /pseudo
     CDS             5450..6591
                     /locus_tag="ACHATZ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(6717..7796)
                     /locus_tag="ACHATZ_001520"
     CDS             complement(6717..7796)
                     /locus_tag="ACHATZ_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8404050.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(7899..8339)
                     /locus_tag="ACHATZ_001521"
     CDS             complement(7899..8339)
                     /locus_tag="ACHATZ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8404051.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(8396..8806)
                     /locus_tag="ACHATZ_001522"
     CDS             complement(8396..8806)
                     /locus_tag="ACHATZ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8404052.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(8986..9420)
                     /locus_tag="ACHATZ_001523"
     CDS             complement(8986..9420)
                     /locus_tag="ACHATZ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8404053.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(9520..10368)
                     /locus_tag="ACHATZ_001524"
     CDS             complement(9520..10368)
                     /locus_tag="ACHATZ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8404054.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            10757..12523
                     /locus_tag="ACHATZ_001525"
     CDS             10757..12523
                     /locus_tag="ACHATZ_001525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8404055.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            12625..13884
                     /locus_tag="ACHATZ_001526"
     CDS             12625..13884
                     /locus_tag="ACHATZ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8404056.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            14031..15332
                     /locus_tag="ACHATZ_001527"
     CDS             14031..15332
                     /locus_tag="ACHATZ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404057.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            15335..16186
                     /locus_tag="ACHATZ_001528"
     CDS             15335..16186
                     /locus_tag="ACHATZ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8404058.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            16199..17014
                     /locus_tag="ACHATZ_001529"
     CDS             16199..17014
                     /locus_tag="ACHATZ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8404059.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            17165..18331
                     /locus_tag="ACHATZ_001530"
     CDS             17165..18331
                     /locus_tag="ACHATZ_001530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8404060.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         6215 a         3266 c         3460 g         5547 t
ORIGIN      
        1 gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga
       61 ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg
      121 ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt
      181 atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc
      241 ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca
      301 gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc
      361 acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt
      421 ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc
      481 ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac
      541 atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc
      601 ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt
      661 ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga
      721 ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag
      781 aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa
      841 aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca aaaactaata
      901 tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa
      961 ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga
     1021 caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca
     1081 gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa
     1141 acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa
     1201 gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt
     1261 ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat
     1321 cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca
     1381 aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca
     1441 catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg agcttggccg
     1501 attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg
     1561 ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac
     1621 ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat
     1681 ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc
     1741 caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta
     1801 atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg
     1861 actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct
     1921 ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag
     1981 atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca
     2041 gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg
     2101 cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga tacctctcaa
     2161 aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac
     2221 ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc
     2281 attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac agaccttcag
     2341 cataagcttt ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg
     2401 gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc
     2461 agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt acgaaaaaca
     2521 ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt
     2581 acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag
     2641 ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg gagaggatca
     2701 aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca
     2761 atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata
     2821 agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc
     2881 ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt
     2941 tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac
     3001 attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct cctatcaaat
     3061 ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt
     3121 ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag
     3181 atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga gaagatttag
     3241 attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt acagggagta
     3301 ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc caaagagatc
     3361 gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg
     3421 caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg
     3481 acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg aatgagacag
     3541 gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat
     3601 gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt
     3661 attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa
     3721 gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt
     3781 gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt
     3841 ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag
     3901 gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt
     3961 gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga
     4021 tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat
     4081 catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg
     4141 gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga
     4201 cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt
     4261 gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa
     4321 tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc accatttctc
     4381 ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg
     4441 tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt
     4501 gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca ataaaaaagt
     4561 caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat
     4621 tctctttaca tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt
     4681 caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat
     4741 cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt
     4801 gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca
     4861 aaccgtacta tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac
     4921 tatagtttgt ttgctgactg aaactgataa tcgaatttgt ctgacagctg aacaaagaag
     4981 tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt
     5041 aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca
     5101 gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat
     5161 ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt
     5221 cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag ataaacgttt
     5281 taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga
     5341 aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc
     5401 tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc
     5461 aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt
     5521 ccacacagag gagacagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg
     5581 aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg
     5641 acaaacagcg accatcaaag caaaaattga ctattcggga gacttagcac ctatactaca
     5701 atcagctgtt acatttgaga atgttgatcc cactttgtcg attggctatg acgagaacac
     5761 tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt
     5821 gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcattt
     5881 aacgccgtat agtcagcggc agacaattga gcaagattca gccatcgact caggaggaga
     5941 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
     6001 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
     6061 aactcaacca gagccagaca ataaatcaaa acctgaaata acccctggag aaaaaccaga
     6121 tgtaactcca gaaccagata cggattctgg aaaccaaaca gtaccggaaa caaatccaga
     6181 cacagataat gaaactgaaa atccagaaaa accagaggta gatccagaag aaaaaccaga
     6241 tgtgactcca gaaccagata cggacgcaag agaccaagga ataccagaaa aaataaacaa
     6301 aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa tcaaatctag caattctaaa
     6361 aataaatgaa gagcagctga ataaaaaatc acgaatattt gatagcgccc aatcagctga
     6421 aaccttaaaa agtagcaagg atacaacctt tgctgtcctg aaacgaaaaa taaacagcta
     6481 ccaaaatccg gagagtcaca aaacaaagtt atactgtgga gcggaatcat tctattaagt
     6541 atagcaacaa tgctttctgc aaaaagattt aaacaaaaca gatctctcta acatttgaat
     6601 aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc agaaaatgac cccaaaatac
     6661 cagattgaaa tctgatattt ggggtcgttc atactttatc tttcttcata tttattttaa
     6721 gggatatctg tttcttttgg aagatggttc gggatctttt tcactttcaa tacatctaaa
     6781 aagaaggtaa agatcggaat gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta
     6841 acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg ttttgctgga catcaacttc
     6901 ggattcagca cataagactc aattagatgg acaatcaaaa tcgtaatcag gatataaaca
     6961 acatctcgga tgcctccttg ggaataagct atgaaagata gaggaataca agaaatgata
     7021 acacctgcta ctgggatcag acttaaaatg aaaatcatga tcgctaaact cagcagctga
     7081 tgaaagccaa atacagccaa ggcgattgta gtgagcaacg tatttaaaag agcgacaaca
     7141 aattgagctt ctaataccaa accaaatgta ttgacaaact tatcggcaaa atagtaaata
     7201 tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc ttgagaatac aattgttttt
     7261 ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca tagcaaacgc cactgccatt
     7321 tttcctatat cctgtaaata gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa
     7381 aaatcgtttc gctcaatata agtatggata tattgtaaaa caggatctgc atcttttggc
     7441 tgatgctcgt aaaaattaat aacggactgg atcatgtcaa atgtttgatg aataagcatc
     7501 gggatatact ttgtgaatgc cagatagata aggtaaacga tcagtgtata aatcatgata
     7561 gctaaaacac ctgtagggat tcggacaaat cgctgaatga tttgaacaag atgaatcatc
     7621 aaataagtaa agataaatgt gagcaaaatg gttgtaatca tacttctaac taagaaaaga
     7681 acaaaaacga caaataataa aacagtgaga cggcgtattt tttcattcgc caaaaaacgc
     7741 tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa taaacacgtt attcattata
     7801 tgatggaata tgaaaataag aaagagcaaa aagaaaaacc gctaaatgac gttatcttat
     7861 gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt ctggtcttga
     7921 tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc ttcctgttca
     7981 agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg caacgcagca
     8041 ccttgagcag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg ctgacgatta
     8101 atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga atccagacac
     8161 aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac taacatcaca
     8221 agatactggg tataggtaag attcagcggc ttgaggaggg gagtgtagtg tctaatgatt
     8281 tctttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag atcttccatt
     8341 tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa aaagtctatt
     8401 cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa tacggacaca
     8461 ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct tcgatagatc
     8521 cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg gaaacttgtg
     8581 cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct aatgcactgt
     8641 taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca ttttcgacgc
     8701 gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatgc acttctccag
     8761 agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaag ttaccacctt
     8821 taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca aagaaatgga
     8881 ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg aatagcaaac
     8941 atatacaaaa aatatcagag acaaaaaata atctcatcat aaacattaca tatatttctc
     9001 aaaaagttgt tttctttctt caaaaattac ttcacgatgg acctgatgaa gcatccataa
     9061 ataaccaaaa ggatctctga acattgagtt agtcacacca aagtccatca tttctgtcat
     9121 ttcttgaatc tctacacagc catttcgtaa tgctttatta tgtgtgtcct cgatatcaga
     9181 gacgatgata ttgaaccaga tcgtatttgg atgttcagca gtaggagcag tcaaaccaag
     9241 agacgtgttt tcatccagca tatggaatct cgtaccatac aaggtaaaaa tgacttcatt
     9301 attgcctcta gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata
     9361 cagttctagt gctttcaaac tatcatttaa tacaaaatcg atttccgttc ctttatgcat
     9421 aaagatctcc tctccttttc agtcgaacag ttactagatt cttttattat accagctaaa
     9481 gaaaacgcat tcaaaaaaat agcggattta cttaccaact cattcgattg gatgataagt
     9541 aaatccgcgt cccattactt ctgctacgct gctgacaatg acaaaggcac ttggatcgat
     9601 ttcttctatc aatggtttca ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac
     9661 ctcttttgac tttcgattat acccgccttt tgcctctagc agggtaaggc cacgtccaat
     9721 ctcagcactt aagcgaaggc ggatgtcttc caaataatgt tcactcatga ccataacaga
     9781 tttctttcga ttgatacctg tttcgatata agtcatgaca attgaagtga ttgcaataga
     9841 taaaatagca taaagaaact cttctattcc aaaaataaac agattaagag aaacaacgaa
     9901 tgcgtctgta aaaaataagc caatcgaagt attcaagtgg aaatattttt taaaaatgag
     9961 gggagggatc gtcgtaccgc cgctagaaga gttgttttta tataaaatcg caactcctaa
    10021 agcaaaaatc gcacttccaa aaatgacaga caacaaccga tctgaagtaa tcattgtttc
    10081 tggaactatt gcaatgatta aaggaaaaac caagcttccg aaaagtactt taaaaaaggg
    10141 ttttttacct aaaaatagaa gagctagtac caacataacg atattcaaca ctaaaataac
    10201 tatagctcgg tcaaagccta aagcagcttc cagtaatatt cctagaccgc tgacacctcc
    10261 agcagcaata tgatgaggcc ctaaaaacat attgatagcg actcctaata tgaagagggc
    10321 aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt tctttcattt attcacttcc
    10381 ttaattcatt aataatactc ttatcttatt ataacttact tttcacctta agttaacttt
    10441 aattaataaa aaaattaggc tagtctaaat tattatgttt cattcttcta gaatatactt
    10501 ctacgccaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt gatcgcttaa
    10561 gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa agttttaagt
    10621 ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac
    10681 atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt catattaaaa
    10741 cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc
    10801 atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa
    10861 aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat ggtatcaaaa
    10921 acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt
    10981 gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag ataacacgga
    11041 ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa
    11101 tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt
    11161 aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa
    11221 tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag actttttcgg
    11281 gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg gcatcaatgg
    11341 catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta
    11401 ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc
    11461 acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc
    11521 accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca
    11581 catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa
    11641 tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc
    11701 agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga
    11761 tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga
    11821 agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca
    11881 agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta cagattcgat
    11941 taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca
    12001 tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca
    12061 tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat
    12121 ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat
    12181 gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga
    12241 tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat
    12301 tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata agctctattt
    12361 cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg
    12421 ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg
    12481 gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac
    12541 aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg
    12601 ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg
    12661 ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca
    12721 gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat
    12781 tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat caagctagta
    12841 gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga
    12901 cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg
    12961 gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact
    13021 tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac
    13081 aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac
    13141 agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact
    13201 gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat
    13261 ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat
    13321 gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga
    13381 gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg
    13441 caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta
    13501 aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat
    13561 gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa
    13621 gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca
    13681 aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg
    13741 atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat
    13801 gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt
    13861 gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga
    13921 acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc tcttgttgcc
    13981 attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag atgcagcaag
    14041 ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg
    14101 ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata ttcgtcctat
    14161 tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt actttgggaa
    14221 gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg caattcatca
    14281 ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat cgagtagcga
    14341 agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata cataatatag
    14401 gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg atttttacga
    14461 tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac ttcaatcata
    14521 ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt atcttctttt
    14581 taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt tggactttat
    14641 gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct caccagaaat
    14701 ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg gtcccagctt
    14761 ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc atcaatactc
    14821 aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa
    14881 cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta ggatttccct
    14941 atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta tatgaagcag
    15001 caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt cctgctgtct
    15061 tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat aatttctcca
    15121 tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct ggttctactg
    15181 atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag tattctatgg
    15241 cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg ctggtattta
    15301 aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt cgagtgctcg
    15361 attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac tgtcagtcat
    15421 tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa atattgcagc
    15481 ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc tattcagtga
    15541 aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa caatgatcgt
    15601 acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt ttatcggccg
    15661 caaatcaagt ctgatgttct ttttagtgat tcagatggta ccgactatgg cagcattgac
    15721 tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt tcttgacaat
    15781 gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat actttgacac
    15841 tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt tcaggatttt
    15901 tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt
    15961 tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac ctgaaaatca
    16021 gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa gagtctctct
    16081 ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct tctttttaca
    16141 aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc agagtatcat
    16201 gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag tgtttgcggg
    16261 acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag tttccttgat
    16321 ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat tagaacaatt
    16381 tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg aacttgaatt
    16441 atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg aaattttggt
    16501 aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg attttgagaa
    16561 ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata cgatgaatta
    16621 tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac gatttttaga
    16681 aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt taagtcttgg
    16741 tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt tcttgtggat
    16801 gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa atttgatgct
    16861 gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc actttgactt
    16921 tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat gggtttttgt
    16981 caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt atgcgacaat
    17041 gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt
    17101 cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt taaggaggaa
    17161 aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct cgctccctgt
    17221 cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca ctcctcccca
    17281 aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta acgcatcttt
    17341 ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta tccaagcatt
    17401 gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt atctaaaatg
    17461 gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta
    17521 tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt attcttgtgc
    17581 ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag tccatcatat
    17641 cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt gtggctgggg
    17701 aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga tcacactaag
    17761 tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag aagataaatg
    17821 ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc atatcacaag
    17881 tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca aagtggtaaa
    17941 agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac aacaaggcgg
    18001 tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc ctggtgttac
    18061 tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg aaagcttacg
    18121 tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag tccaagatca
    18181 agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt tacaagcttg
    18241 tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg
    18301 aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt taaagactat
    18361 ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct aacttctagg
    18421 attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt ttggttcttt
    18481 aattttta
//
LOCUS       DAYYAJ010000055        18328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000055 DAYYAJ010000000
VERSION     DAYYAJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.055"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACHATZ_001531"
     CDS             19..540
                     /locus_tag="ACHATZ_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8404061.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACHATZ_001532"
     CDS             637..858
                     /locus_tag="ACHATZ_001532"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8404062.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACHATZ_001533"
     CDS             complement(1015..1179)
                     /locus_tag="ACHATZ_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8404063.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACHATZ_001534"
     CDS             1282..1803
                     /locus_tag="ACHATZ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8404064.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACHATZ_001535"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACHATZ_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACHATZ_001536"
     CDS             complement(2270..2428)
                     /locus_tag="ACHATZ_001536"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8404065.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACHATZ_001537"
     CDS             2521..3282
                     /locus_tag="ACHATZ_001537"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8404066.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACHATZ_001538"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACHATZ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACHATZ_001539"
     CDS             4837..6057
                     /locus_tag="ACHATZ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8404067.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACHATZ_001540"
     CDS             6092..6769
                     /locus_tag="ACHATZ_001540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8404068.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACHATZ_001541"
     CDS             6766..7962
                     /locus_tag="ACHATZ_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8404069.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACHATZ_001542"
     CDS             7977..8300
                     /locus_tag="ACHATZ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8404070.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACHATZ_001543"
     CDS             8304..8639
                     /locus_tag="ACHATZ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8404071.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACHATZ_001544"
     CDS             8636..9031
                     /locus_tag="ACHATZ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8404072.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACHATZ_001545"
     CDS             9028..9399
                     /locus_tag="ACHATZ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404073.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACHATZ_001546"
     CDS             9415..9975
                     /locus_tag="ACHATZ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8404074.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACHATZ_001547"
     CDS             10102..10413
                     /locus_tag="ACHATZ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404075.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACHATZ_001548"
     CDS             10437..10628
                     /locus_tag="ACHATZ_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404076.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACHATZ_001549"
     CDS             10628..14473
                     /locus_tag="ACHATZ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8404077.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACHATZ_001550"
     CDS             14474..15346
                     /locus_tag="ACHATZ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8404078.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACHATZ_001551"
     CDS             15343..16392
                     /locus_tag="ACHATZ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404079.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACHATZ_001552"
     CDS             16396..17220
                     /locus_tag="ACHATZ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404080.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..>18328
                     /locus_tag="ACHATZ_001553"
     CDS             17238..>18328
                     /locus_tag="ACHATZ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8404081.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIK"
BASE COUNT         6339 a         3150 c         4128 g         4711 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagat
//
LOCUS       DAYYAJ010000056        18248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000056 DAYYAJ010000000
VERSION     DAYYAJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.056"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..1660)
                     /locus_tag="ACHATZ_001554"
     CDS             complement(152..1660)
                     /locus_tag="ACHATZ_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8404082.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1819..2010
                     /locus_tag="ACHATZ_001555"
     CDS             1819..2010
                     /locus_tag="ACHATZ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404083.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2213..3130
                     /locus_tag="ACHATZ_001556"
     CDS             2213..3130
                     /locus_tag="ACHATZ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8404084.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3123..4196
                     /locus_tag="ACHATZ_001557"
     CDS             3123..4196
                     /locus_tag="ACHATZ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404085.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4344..4862
                     /locus_tag="ACHATZ_001558"
     CDS             4344..4862
                     /locus_tag="ACHATZ_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404086.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4843..5235
                     /locus_tag="ACHATZ_001559"
     CDS             4843..5235
                     /locus_tag="ACHATZ_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8404087.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5311..6246
                     /locus_tag="ACHATZ_001560"
     CDS             5311..6246
                     /locus_tag="ACHATZ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8404088.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6379..7158
                     /locus_tag="ACHATZ_001561"
     CDS             6379..7158
                     /locus_tag="ACHATZ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8404089.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7151..7468
                     /locus_tag="ACHATZ_001562"
     CDS             7151..7468
                     /locus_tag="ACHATZ_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8404090.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7712..9322
                     /locus_tag="ACHATZ_001563"
     CDS             7712..9322
                     /locus_tag="ACHATZ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8404091.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9479..10327
                     /locus_tag="ACHATZ_001564"
     CDS             9479..10327
                     /locus_tag="ACHATZ_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8404092.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10558..11037
                     /locus_tag="ACHATZ_001565"
     CDS             10558..11037
                     /locus_tag="ACHATZ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8404093.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            11070..13298
                     /locus_tag="ACHATZ_001566"
     CDS             11070..13298
                     /locus_tag="ACHATZ_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8404094.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13427..15070
                     /locus_tag="ACHATZ_001567"
     CDS             13427..15070
                     /locus_tag="ACHATZ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8404095.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15143..15688)
                     /locus_tag="ACHATZ_001568"
     CDS             complement(15143..15688)
                     /locus_tag="ACHATZ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8404096.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15711..16196)
                     /locus_tag="ACHATZ_001569"
     CDS             complement(15711..16196)
                     /locus_tag="ACHATZ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8404097.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16186..16998)
                     /locus_tag="ACHATZ_001570"
     CDS             complement(16186..16998)
                     /locus_tag="ACHATZ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8404098.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17137..>18248)
                     /locus_tag="ACHATZ_001571"
     CDS             complement(17137..>18248)
                     /locus_tag="ACHATZ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8404099.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5941 a         3315 c         3646 g         5346 t
ORIGIN      
        1 tttagagtga aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta
       61 aaaagtgtaa cgtcttagta aagtctataa aaatacgaaa ctatcgatta agctaatagt
      121 ttcgttttca acatttataa gtaaactttt tctattggta atatagattt tcggaagggt
      181 agacaggtgc gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat
      241 tgaccatcac agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa
      301 cttgagcgat tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc
      361 ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa
      421 ctaatggtga caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta
      481 agataactgc cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat
      541 ctggtaattc gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct
      601 tatctgcata gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt
      661 cttccataat cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt
      721 cgcattctgt ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg
      781 tatcatccac gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa
      841 cagattcttt accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat
      901 cccgattata gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat
      961 tgacatcttt tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg
     1021 gaacattcca tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc
     1081 tttcatgata cagttggttc aaggaagtag cgagctttcc acttcctggg ccaggagctg
     1141 tcactacaac gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg
     1201 agacgatttt ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga
     1261 tgtttcgtct ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag
     1321 tgataacgac actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga
     1381 tatccatatc ataagtaatc ccataatcac cacggatctt attacgttca atatctccag
     1441 catagacgca aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg
     1501 catcttcttc aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact agcttcccac
     1561 caaattctaa atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct
     1621 cttcaatata tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac
     1681 taaagcattt tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa
     1741 aacaaagaaa atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt
     1801 tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat
     1861 atccgttcga tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg
     1921 acgtttactt atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat
     1981 ttcacaacaa gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt
     2041 ctgtcctatc agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc
     2101 agcctgtttt atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta
     2161 ctattgaagt attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga
     2221 gccagataca aatgcaaatt atgagccaga cgaactagag gagacgaatg cggatcagga
     2281 tacaggacaa gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc
     2341 ccaattatta gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga
     2401 gatctaccgt ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa
     2461 tcacgagatc gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc
     2521 ttttatggaa caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta
     2581 ttaccatgat cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc
     2641 taagatcgat gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa
     2701 aatcaatgag ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga
     2761 acaaataaaa gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa
     2821 tcgtaaaaaa atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa
     2881 tgaaaaatta ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga
     2941 agcattgaag caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca
     3001 aaggcaaatc agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt
     3061 cttgatgaat tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga
     3121 gtatgaatga acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg
     3181 aagaaacgaa cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa
     3241 tccctatgct agaggaaaca aaaatacacg aaaaagaaaa agtaactccg acgaatacga
     3301 atactgcaga aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa
     3361 aagagcaaga tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg
     3421 atttacgtat gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg
     3481 atcgttttat attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat
     3541 acttcacgta tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag
     3601 cttatgacac atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg
     3661 aattagtctt ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga
     3721 gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt
     3781 ctttgatccg acactggtca gtggatcgag tattctctgc agggcagatc acgatattca
     3841 atcacaaatt gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga
     3901 cattactaga caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga
     3961 atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa
     4021 agatcaacga aactacttat caagtacgat tagataaagg acagtcttta acgataacaa
     4081 caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca
     4141 ttgatttctt tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg
     4201 ataagttgga ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta
     4261 tccagaataa ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg
     4321 atgaaggagg gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt
     4381 ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga
     4441 ctgctatttt tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga
     4501 gtctttctat tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat
     4561 ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc
     4621 aaaggaatct atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag
     4681 gtatgaccgt ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc
     4741 aaggggtgtc gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc
     4801 tgccagtcgt caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat
     4861 agtttgcttg caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca
     4921 caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta
     4981 caagaaaatg gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta
     5041 ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt
     5101 gaagggcact cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa
     5161 agtgctacta ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat
     5221 ttgtctaaac tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt
     5281 gagttcagtt gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt
     5341 tccgtaattg ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat
     5401 gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc
     5461 aatcagattt tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat
     5521 caatttggtg aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc
     5581 ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat
     5641 gaagtgggta atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga
     5701 agttcgttac gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg
     5761 atgcgctttt tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg
     5821 tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg
     5881 attgctgcat ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc
     5941 attttcctag caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat
     6001 ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa
     6061 acagttcatt taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac
     6121 aaagagaatg aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt
     6181 cctgacaaga agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt
     6241 aaataaaacg tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa
     6301 ataaaacggc tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta
     6361 attaaaggag atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat
     6421 gcagtgatgc cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg
     6481 caaaaagttg gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt
     6541 ggcgcgcagt ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta
     6601 ttgatggttt tctttttaga attacgctat acattattag gagcaagtct tgccggtttt
     6661 atgaaaaaag aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac
     6721 tatgcggtga actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta
     6781 tatgtcaatt ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc
     6841 ggctcggtga ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt
     6901 ttcatgttta tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc
     6961 gtattagggg tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca
     7021 gtcattgctt catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag
     7081 aaactaagag accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag
     7141 gccaatcacc atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc
     7201 atatatcccg cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt
     7261 cagtgactgg atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt
     7321 tttcattact catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct
     7381 agtagcaggc gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc
     7441 gtctgtcttt ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg
     7501 acataagttg ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca
     7561 agtttattcg tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt
     7621 ttaggaatta gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt
     7681 attttatgaa agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt
     7741 ttggctaacc tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg
     7801 gctaagtatg atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg
     7861 ctatcttcct tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc
     7921 aaaaaaagta gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag
     7981 aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt
     8041 tgctccttta ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat
     8101 cagaagttta tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt
     8161 ccaaggaaaa aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg
     8221 agtgatcgtt atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt
     8281 agaagctgta ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca
     8341 tatgtatatc gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag
     8401 ccgttatttc gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc
     8461 gtttattttt gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa
     8521 accactgctt cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag
     8581 ccgtgcttct gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt
     8641 agctataagc agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga
     8701 aaaaacagta ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg
     8761 aatgtcttta ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt
     8821 cggatatttc caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc
     8881 tacgatgctt caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat
     8941 tatcctgaaa gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag
     9001 cttatcaaat ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa
     9061 agaataccga atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc
     9121 caccctgatt atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc
     9181 aatggaatca aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt
     9241 agtattttta ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca
     9301 tcatagcaga agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc
     9361 aactcgtatg attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct
     9421 tacttatgat atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat
     9481 gaaacttgca gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc
     9541 cgacttattc gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat
     9601 cgatatcgaa gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac
     9661 ttcacaacct gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta
     9721 tgacacgatt ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt
     9781 tgcgatgaaa gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag
     9841 tatgccgatg ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct
     9901 tgatgaaata cttgcacata tcgatcgaat ccgagataat acctacgctt atctgattgt
     9961 agatgatttg aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg
    10021 cagcttatta aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga
    10081 aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa
    10141 taacgaaata caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc
    10201 gattgcggag aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca
    10261 tttcggacca gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc
    10321 gcaataattc cttttattca aaaaacatac caaaaatatt ccaacatcat cttggacttt
    10381 ctacatgtta ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc tatcaatgaa
    10441 gctctttttc tttgcaatcc tcacttcaat cctctataat gtcgcatata gtttagtaaa
    10501 tgagtaaact actaatttac ttatttacta aaaataaaag gatgaggagt taaaataatg
    10561 gaaataccaa caaatttaaa acataaacca gtaatcgttg tagaagacta tgataaagtt
    10621 gatggaagaa atgcattaaa tacggatgca aaagggcttt cactgggcct tgctcagtgg
    10681 aatgatcgtg gaaaagtcga tatctcaggc aaggtatggc gacatactgg agaaaaatgg
    10741 tcacgacagt cggaagaatt accaatcaca cgtattttag atttagcgat cttagctgca
    10801 caaggaagtt tatacttcca agatgcttat cgttatgaaa aattttatga tccagaaaat
    10861 ccagtcgtag acatcattgg attacaaggg aaccgaatga cggtagaagt agataccaaa
    10921 aatccaaaga tcgatgaaga catcattttg tattacgata cactacaaaa agacggagaa
    10981 ctgattgggg aacgattccg tattttaaaa cgtcttttag atgagctagg atattaatag
    11041 acagagatga tcaaaagggg aagagaagca tggagaattt ctcactaaca gaaaaccgaa
    11101 ccttttcacg gctagaaaag caaatgatct ggaagaaacc gaaaagccat cggacaagtg
    11161 cagaagaatg gcggatcgcc tccgaaatca aagggaattg gcaagatcct gaaatgaaaa
    11221 tcagtaacgt acttttggaa ggagatgccg ggtcggggaa gacacaatta gccaaagcac
    11281 tatctgctga tctgcaactt ccctatacaa aagtgacctg ttttgcagat atggacaaat
    11341 cagatgtttt cggagcctta ttacctgtag tagattctaa taaggaacaa gaccaagaat
    11401 tactagaagc tatttaccaa acagatacac ttgaagctgt actgacactg atccagaacc
    11461 attacggcat tgatcctttt tctgctaaag aaaaattagc agagctagtt caaaggatcg
    11521 aagctgatta tccagatgca atccattaca gctattaccc ttctgaaatc gttcgagcga
    11581 ttgaaaaagg atatttatta gagattcaag agccgactgt catccgagat gcctctgttc
    11641 ttgtggcact taactcagcg ctcgaaccaa atgggatgtt gaatttaccg acaggaatca
    11701 ttcgtcgcca tccagattgc gtcattgtga tcactacaaa tcgaaattac caaggaaatc
    11761 gcccattaaa tgaatcttta agagatcgga tgcaacatgc ggaaaaaatg gatcttcctg
    11821 ccttagaagt catggcagaa cgtgcgatgg cgaaaactat gatccaaaaa caagaacttc
    11881 tcttaaagat ggcagagatt atccgtttgt tggatgaaac tgcaaaagct aacgccatca
    11941 aaggtgtagc aggaatgcgt tcttattttt attgggcaaa tacctataag caaggacaag
    12001 atttactcca gtcgatttat ccaaaagttt tgtataaatt atccactgat tcagatgaat
    12061 tggcaattct tgaacaagct ttgtctgaca gtggattgct actggaactt caggacatat
    12121 tgagaaaaga aaaatggggt catcctcaat ctgagcatac aaaaggaaga accattagcg
    12181 ccgaagaagc aaatgaacga aacatcactg aaaatgctga agaaattctg cgcttagatg
    12241 aaaagaaatc gttgtctgct gaagaaccaa atgagggaga agagaaacaa gaagaaacaa
    12301 tagaaaaaaa cgagattcct cctcaaatgc aaaaagaacg cagtagcgat atcgaaggaa
    12361 aaaatcaaaa agaacaacaa acaggagatg attctcaaga aatgtctgct tctgacatgg
    12421 aagcacatga taaggaaatc aagaagcaac tgaataaaga agcgcgacaa atcatgaagg
    12481 aaacgattca tgaaaaagaa ggtctcatcg ttcaccgacc agctttccaa ggaaaaacgc
    12541 cggaagcacg tgagctacac atgcaagtaa tgccaatagt agaatcactt agccggcaaa
    12601 ttttagagtt actggataat gaacaaacag agacgtacca aaaaggaaaa tacgaaggtc
    12661 agcggttcaa tgcaagcaaa gtagcatatg gtgatttgag aaattttgac aaaaagaacc
    12721 cgccacatga gcaaccttct ttagcggtag ctgtacggat cgatgaatct gggtcaatga
    12781 tacgggatga tcggatcgaa gcggcaaaaa aagcagcgat tgccattgcc gaattttcta
    12841 agaaagtcga tatcccattg ttgatttacg gagatacagc agaccgttcg tcaagagaaa
    12901 agacttcttt gttttcttat aaagaatttg aagacggatt tcaatggtta aatgaaaaat
    12961 tagtcactat gaaaccaagg caaaacaatc gtgatggtgc tgcacttcgt ttagcagcag
    13021 aaaaattaaa cagacaatcc gcaacaacca aactgttgct taatattagt gacggtcagc
    13081 caaaagcgtt gccggattat acaggcaaaa aagcaaaaga agatatccag tctgttttga
    13141 aagaatatga acgtcaagga atcctatttg tctccgcagc tattggccaa gacaaagaag
    13201 aaatcaagtc gatctacggt gaaagtcgtt ttgtagatat tactgacttg aatgaatttc
    13261 ccaagcaaat gattcaactg attgcccgct atctatgata aaataaagct caacgaacgc
    13321 acttagcaga ttcgttgagc ttttcgtttc tagttaccgg taacaaggat acttctaaaa
    13381 tagaatcggt tataatcaag agggaccgtt aaaggagtga aacgatatga tacaagaaca
    13441 aaaatggtgg aaagaagcag taggctatca aatctatcct gctagtttca aagatagcaa
    13501 tggcgatggt caaggagaca tcaatgggat ccgtgaaaaa cttaattatt tgaaagatct
    13561 tggtatcggc tttatctgga tcaatccgat ctatcaatct ccatttgtcg ataatggtta
    13621 tgacgtaagt gactatcaag atatagaaga gagattcgga acgatggaag attttgattt
    13681 attgctaaaa gaagcacatg aattagggat caaagtgatt atggatcttg tcatcaacca
    13741 tagttcggat cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa gcccgtatcg
    13801 cgattattac atttgggtcg acggggtaga tggcaaagaa ccgaataatt ggacatcgat
    13861 tttcggcggt tctgcttggg agtattctca tgaaactgga cagtattatc tgcatgtttt
    13921 tgcaaaagaa caaccagact taaattggga aagcgagaaa ttgaaagaag aactattcaa
    13981 tatgatccga tggtggttgg ataaagggat agatggattt cgtttagatg cgatttctca
    14041 tgtaaaaaaa gatgaatatt cagtaaaagc cacagagaac cctttctcac cctttcaaaa
    14101 tgtctcagga atcgaagaac atttgaccga tttgaaacat gtttttgaag agtacgatat
    14161 catgactgtc ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat gggtagggaa
    14221 agacggttat ttcgatatga tattcgaatt tgatcatatc catatctggc agcaagaaaa
    14281 agaagggcaa ttagatgtcc ttaaattgaa acttgcatta agcgcttggc aaacttcatt
    14341 ggatggtatc ggatggaacg ctttatatat ggaaaaccat gatgttcctc gtgccgtttc
    14401 agtgtttgga gatactcgtc ccgatttttg ggctatgtct gcaaaagcaa tcgctatgat
    14461 gtatttcttt ttgcaaggta cgccatttat ttatcaagga caggaaatcg gaatgacgaa
    14521 tatgcccttt gaatcgatcg atcaagttga tgcagtagat tcgaaaagac tttataaaag
    14581 gctgcttgcg gaaggtaaga caagagaaga agcattggat atcattcgtg aaacaacgag
    14641 agacaacagc aggacaccca tgcaatggac ttcggaacaa tatgctggtt tttctacaca
    14701 tgaaccttgg ctgatcacta atccaaatac caaaacgatc aatgtagaac aacaagaata
    14761 tgaaccagag tcggtcctgc aattctataa gaacatgatc cgcattagac aaaccaataa
    14821 aggtctgata tacggttctt ataaagaata tcttcatgaa catccacaac tttatgtcta
    14881 tgaaagatat ttagaagatg aacaatatct tattatggta aacttaacag aatcacttgc
    14941 tgactatgaa cttccaaaag aagcagatca gtcctggaca ttactcttgt caaattcctc
    15001 tagcggagaa tttgaagcta aaggcatact agcaccttat gaagctcggt tatataaaac
    15061 aaacaagtaa aacttcctcg ttaaagttca ttagttggac tttgcgagga agtttttcag
    15121 attgctctgt caattactca tttcaataat tcgctctagg accaccgata tatttaggac
    15181 gagagcgaag ggctgtcacg tgagcttgtc caagggtttt caaacgaggt ctgacaggta
    15241 cttggacatg tttgatatgc ataccgattg atgtgtcacc gatatcgata ccggcttgtc
    15301 caactacgtg ttccacttcg acaggatcat tgaactggtc aaaagcagca acagaacaag
    15361 cacctccagc atgtagtgca ggaacaacag agacaatttc gaaatttttt gcttctgcca
    15421 ctgttcgttc tactactaat gctcggttca aatgttcaca accttggaca gctagcgcaa
    15481 tttctttagg atccaacaac tcttttaacg tacggatgat ccattgacca acttccgcac
    15541 tggaattttt tccaatatgt cctccaacga cttcgcttgt gctgcatcct aaaacaaaaa
    15601 gatcaccttt tttcaagtgt gcctcagcca atatgtcatt aactatttca gtcaattggt
    15661 cttttaattc tttttctgac aacttcattt tctaaaacct tctttttcaa ttaatattgc
    15721 ttccgtgctt gaatacgttg aagacttgca gttataggaa aggcaagtcc cattccaatc
    15781 agattttgga agatgtttcc tggtatagaa gccactccag ccggccaatc atataaaatc
    15841 caaccggcga taccatagcc gatgatcata actaagcttc ctaataaaag ccctaatccc
    15901 cacatacgag tagaagattt gctgaaagaa ccagcaacat atccttgtaa cccgtggatc
    15961 actaatgaaa aaactgccca ctgcggatat cctgaaatca aatcgatcaa tagaccactg
    16021 gaagcaccaa caactaatcc acccgttgga ccataaagaa aagcagcggt atagatcccg
    16081 gcctcgcata atgtcacaaa tccattcgta gccggtacag ggatcaaaat tgttaatgaa
    16141 agggcaactg tcaacgcaat cagcatagca tagatcgtta attgcttagt tttcatttct
    16201 cattcctcct ttttgaatac cgccagacca gacacttccg gtatgatcgt tcatcagcac
    16261 gccattagaa atacaatggg caacaaattt tttactttct gctactgctt tggtaaaatc
    16321 tgcccctaac acaagattcg ctgttattgc agaagaaaag gcacaaccgg ccccgtttac
    16381 tgttgtagtg tcgatttttt tatgagaaaa tacctcataa tgatcaattg aatagagaag
    16441 atccacagct tcatctccag ccatccttgt gccgcctttg atcacaatgt tttttgttcc
    16501 tgaagcagtg agttctttag cgagatggat catttctgtt aacgaagagg gaagagcttg
    16561 accagaaagt aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa gtggaaaaag
    16621 ttcttcgatg agctgcgaaa tatacaattg attgatcaca gcttcagttt ctttgaatgc
    16681 caaaacaggg tctaaaatga ttgggatatt ttgttttgtt tttatgaagt ctcgaacaac
    16741 catgattgct tcacttgaat tcaataaccc aattttgata ccatctaatg aagtcatttt
    16801 atcaatcgaa actaactgtc gtttaagcaa ttccaaagat atagattcca gcataaatcg
    16861 ctcattaata tctagtgccc ctatagaagt caaggcgctt agtccgaata gaccaaaatt
    16921 ctcaaaagtt tttaaatctg tttggattcc tccaccagca aatggatcag aaccgccaat
    16981 tgtcaagatt cttttcataa cattttcctc catgaataag agtataacga aattttcaaa
    17041 aaagaaaaac agccaattgg catattttta tctcatccaa tttaaaaaaa caaaaagaaa
    17101 agaaagacca aaatggatct ttctttcctt aaatagttag gacttttgtg attttaattc
    17161 tttctcgatt tgaagccgtt gcactttcat tgtagctgtt cgcgggattg catcaaaatc
    17221 acggatgata gggacgttca agcgaggaag ttcatggact tgctcccacc atcgattcca
    17281 atccatttct ttatcaggag ctaaagcaat aatcggttgt ggagaatgat ttttatccct
    17341 tacaatcact acttctgcca aaaaatctaa cgaatctaat aaaaagtctt caatagctaa
    17401 tgtactattg atcgtttcga tcaaatccac ttgccgatct ttcaaaaata ggtggccatg
    17461 cttatccata aaaccgtaat ctccgctatc ccaccaatcg ccataaacat tttcctggaa
    17521 ccgtgcatct tccttgtaat aagtcaaagc ccgtccttta gacagtaatt gtatatgacc
    17581 atctgtgttt tcaggtagga cattcccaac tgaatctgta atccgagcgc ttgtcatgtc
    17641 ttccagtcca acgcccatat ctcttgcatc acttgttttc aaactctcta gtgtatgagc
    17701 cttaagaatc attggtccgc attcactttg gccatatact tgaagaaata ccgcatcctg
    17761 tgccatcgac gttcgcaaaa atgtcttcat tgtctggttg tttatagcat caaaagtgga
    17821 gtgatagtat cggatacccg aaaaagcttc tggatgctcc ttagcagtaa aacgccactg
    17881 aacaaagtta ttcggatggg tctctagtgc aattggccgg tgagcttcca acatatgaac
    17941 aacttttgaa ctttgtgcgt ttgctaaagg catcatagga aatcccatgg acatcaaaga
    18001 agagatacca atattgaatc tggaatgcac tggagagatg tgaaaagcga ttaatttctt
    18061 ttccgaaatt tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca tagaatgagc
    18121 agaatgacaa ataagtttag ggataccagt tgtaccagaa gtatgggtca tataagaaat
    18181 ttcatttttg tctaaaaatg attgagaaac agacaaagca ggttgttcca ttaaataaag
    18241 aataggaa
//
LOCUS       DAYYAJ010000057        18057 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000057 DAYYAJ010000000
VERSION     DAYYAJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.057"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACHATZ_001572"
     CDS             complement(<1..229)
                     /locus_tag="ACHATZ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8404100.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
     gene            543..773
                     /locus_tag="ACHATZ_001573"
     CDS             543..773
                     /locus_tag="ACHATZ_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8404101.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            870..2171
                     /gene="murA"
                     /locus_tag="ACHATZ_001574"
     CDS             870..2171
                     /gene="murA"
                     /locus_tag="ACHATZ_001574"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8404102.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            2184..2360
                     /locus_tag="ACHATZ_001575"
     CDS             2184..2360
                     /locus_tag="ACHATZ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8404103.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2495..2791
                     /gene="yidD"
                     /locus_tag="ACHATZ_001576"
     CDS             2495..2791
                     /gene="yidD"
                     /locus_tag="ACHATZ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8404104.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2976..3443
                     /locus_tag="ACHATZ_001577"
     CDS             2976..3443
                     /locus_tag="ACHATZ_001577"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404105.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3528..4412
                     /locus_tag="ACHATZ_001578"
     CDS             3528..4412
                     /locus_tag="ACHATZ_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8404106.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4526..4762
                     /locus_tag="ACHATZ_001579"
     CDS             4526..4762
                     /locus_tag="ACHATZ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8404107.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4850..4930
                     /locus_tag="ACHATZ_001580"
     tRNA            4850..4930
                     /locus_tag="ACHATZ_001580"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4884..4886,aa:Tyr,seq:gta)
     gene            4937..5008
                     /locus_tag="ACHATZ_001581"
     tRNA            4937..5008
                     /locus_tag="ACHATZ_001581"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4969..4971,aa:Gln,seq:ttg)
     gene            5133..5303
                     /locus_tag="ACHATZ_001582"
     CDS             5133..5303
                     /locus_tag="ACHATZ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404108.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5523..6230
                     /locus_tag="ACHATZ_001583"
     CDS             5523..6230
                     /locus_tag="ACHATZ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8404109.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(6289..6933)
                     /locus_tag="ACHATZ_001584"
     CDS             complement(6289..6933)
                     /locus_tag="ACHATZ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8404110.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            7059..7346
                     /locus_tag="ACHATZ_001585"
     CDS             7059..7346
                     /locus_tag="ACHATZ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404111.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7454..7774
                     /locus_tag="ACHATZ_001586"
     CDS             7454..7774
                     /locus_tag="ACHATZ_001586"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8404112.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7855..9168
                     /locus_tag="ACHATZ_001587"
     CDS             7855..9168
                     /locus_tag="ACHATZ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8404113.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            9165..9884
                     /locus_tag="ACHATZ_001588"
     CDS             9165..9884
                     /locus_tag="ACHATZ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8404114.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            10012..10563
                     /gene="raiA"
                     /locus_tag="ACHATZ_001589"
     CDS             10012..10563
                     /gene="raiA"
                     /locus_tag="ACHATZ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8404115.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10785..13319
                     /gene="secA"
                     /locus_tag="ACHATZ_001590"
     CDS             10785..13319
                     /gene="secA"
                     /locus_tag="ACHATZ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8404116.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13445..14546
                     /gene="prfB"
                     /locus_tag="ACHATZ_001591"
     CDS             join(13445..13516,13518..14546)
                     /gene="prfB"
                     /locus_tag="ACHATZ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8404117.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14651..15337
                     /gene="ftsE"
                     /locus_tag="ACHATZ_001592"
     CDS             14651..15337
                     /gene="ftsE"
                     /locus_tag="ACHATZ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8404118.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            15330..16214
                     /gene="ftsX"
                     /locus_tag="ACHATZ_001593"
     CDS             15330..16214
                     /gene="ftsX"
                     /locus_tag="ACHATZ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8404119.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16366..17814
                     /gene="cls"
                     /locus_tag="ACHATZ_001594"
     CDS             16366..17814
                     /gene="cls"
                     /locus_tag="ACHATZ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8404120.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         6034 a         3047 c         3903 g         5073 t
ORIGIN      
        1 cagacacatc attcagtaac aaccgaacgg ttgtcgtccc ccaacgaatg atcctagagg
       61 ggacgacaca aagacaatgg gaacactctt ctggcttgta cagaagagtt cctttgtaaa
      121 tcaagcaaat ttgccctttg tatttttctt tagttaaaca attttcctcg aaatagatat
      181 ttaggtctag aatatttagg gtatctttga taagctgagt atgagacatc ttgaatcctc
      241 ctatgaattg gtagccaaat taattctaaa ggatttcagg gtgtcttttc tatatttttt
      301 tatacaaaaa agatgttggt gaagttcacc aacatcaaaa agtatagagc ctttatttgg
      361 aataagggac tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt
      421 atttcctaaa aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat
      481 acattttatg gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca
      541 ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct
      601 acttagcctt ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata
      661 atacgcaaat tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta
      721 attttttctt agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa
      781 aaaaaggtac tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat
      841 gctcattgga attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc
      901 aattaaatgg aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg
      961 ctagtttgtt agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg
     1021 tcttcactat gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa
     1081 agaatcaagt aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg
     1141 ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg
     1201 caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa
     1261 aaggatttca agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg
     1321 cggatgaatt gatcggtaac acgatttatt tagatttccc tagtgtaggt gctactcaaa
     1381 atatcatgat ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg
     1441 aaccagaaat cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag
     1501 ctggtactga aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa
     1561 tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca
     1621 atgtgctgat tgcagatgct atctctgaac ataaccgccc attgatttct aaattgatcg
     1681 aaatgggtgc agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata
     1741 tcttacctac agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc
     1801 aaatgacagc tatccaatta gttgcagaag gtacaagtgt agtgactgaa acagtgtttg
     1861 aaaatcgttt ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg
     1921 gaaatgtagc aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag
     1981 acttgcgggc agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg
     2041 tgcgtaactt gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat
     2101 taggtgcaga cgttgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa
     2161 cgattgctta aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa
     2221 agattttaat ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat
     2281 atggtgtcat cggtggcggc aatccattta aagtatttca gccaagtttg tggattcata
     2341 ttcgagactt ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag
     2401 ccatgaagca gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc
     2461 tttttttagt aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt
     2521 tagaggatat cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac
     2581 ctgttccaat tatatgataa aagcaattca aacccatggc gcgctaaaag ggacggtaat
     2641 ggggactgca cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc
     2701 attgacattc cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt
     2761 tgtgaaaaat acgggtagca ctgagcgata aagaaacaat ttggaaaata gcttcttata
     2821 ttttgcaaaa ttgtgagtta ttgccgaagt gttggatttt gaacaccgtc aaaaaaaagg
     2881 ttgtgaaaaa tacggctagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt
     2941 aacaactccg caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag
     3001 atttaccatg gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg
     3061 tttccagagg gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct
     3121 atgtggcaag ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag
     3181 gaaaggggat tggcggaaaa ttacttgaga cagcttttca gaagctttca caactcgctt
     3241 ccagtcaaac tcagatcatc atacgaaccg gcagtactag cagtgcggcg ttacatctct
     3301 atcaaaaaat gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact
     3361 ctgagcccat ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact
     3421 tacctaaaaa tagctttaac taataaaatt tcaattcaag aaaatgcttt caaatgctaa
     3481 atagatacat tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag
     3541 tttttgattt tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag
     3601 gtaagggaca atttgcaacg cctgaatctt gggaagaaat gaaagcgcaa ttatttcaag
     3661 tttataaaaa aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag
     3721 tagtgaataa tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat
     3781 ttaatatttt ccgagatttg gaaactctgt ttcaacttcc agtaacgatt gaaaatgacg
     3841 ccaactgtgc cggaatggct gaattttatg aaggtgctgc ccgcgattat caagatgttg
     3901 cttttgttgt ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaatcaata
     3961 aaggggcaca tctttacggt ggagaatttg ggttgatgtt cttaaatggc gataagacat
     4021 tcagtaaact tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg
     4081 ataaaaatgc ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag
     4141 caaaagaaga agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat
     4201 tttcgtttga tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca
     4261 ttgatgagat caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg
     4321 aaccgcagat tttattatgt gaatacagaa acgatgctaa tcttgtaggt gcagcagcaa
     4381 actatctcgc tgtacgtcaa ggggagaatt aagagcttta gagaaggtgc agcttgcata
     4441 aaagaagtga atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt
     4501 atgataaaat atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg
     4561 tatagaagaa gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat
     4621 agggaaaaaa atgattggtg agatcttagc ctatggacca aaagaatatg aaattttctt
     4681 tggtgaagaa gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg
     4741 acactggaat ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa
     4801 atctggaaaa ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc
     4861 gaagtggcta aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca
     4921 ctctcctcca tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     4981 tgcgttggtt cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga
     5041 gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgatt taagatagag
     5101 ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag
     5161 cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa gtgaatattc
     5221 cgaaaaaagg cggaaaagcg gtccgggtaa aatcaaaacg cccaacgaat catacaaaaa
     5281 aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga
     5341 ttttagggtc ttttgttatt ttctaggttg gacatatcta aaaaattgtt tctgacaata
     5401 ggagtaggaa gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt
     5461 ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg
     5521 ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg
     5581 aacaaggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat
     5641 tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct
     5701 tagaaagaaa gatcggttcg ggtacatatg ttgcacgaga aaaagtacaa gaaaaaatgt
     5761 ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga
     5821 caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta
     5881 aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt
     5941 ttgaagttgc cagtgtgcca caaaagctga tcgatggcta cagcaaagca gagattactg
     6001 aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa
     6061 cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag
     6121 atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg
     6181 tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg
     6241 atccctgatt ccgtattaga aataaaagag aatcagaatc acctgttttt atatatgatc
     6301 agttaccagt tgctcgtgat aagaaacagg accctcttca aattggactt gtccgttcag
     6361 cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac
     6421 gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt
     6481 ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt gcaaggtacc
     6541 ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg ctcgtattaa
     6601 tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag ctacattgat
     6661 cagttgattt tttttgagga cttccaacat cggtatgccc gccgttccac ttggttctcc
     6721 atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc tgcagttatg
     6781 gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt cgtcctctga
     6841 ttctattcgt ctaagagaac agataaagcg tgattttttg atctcgatct cactttgtcc
     6901 attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt cattttcttt
     6961 attcagtata aataaagttc taggttgttt caacactgta acggttgcgc aattgctgta
     7021 gtatgcgata ataaagacaa aggcaggtgg atctggggat gcaggaacga atgaaaaagt
     7081 acgatgcaat tacgcattat ttgaagaaca atggcggctc tcaagtaacg ttgaccttta
     7141 cccaatttga cgaactgctg tttcccagta acggacttcc caaaacagca agggaaagta
     7201 cagattggtg ggcaaatgat tataaacacc ctgaaaaagg tgcctatggc tggataaatg
     7261 caggctatga agtagttgtc atcaatttgg acaaagaata tgtcgtgttc aataaattag
     7321 tgaaatctag ttggctattt gattaaataa accgcgttta gaaaacgttg acaaatttct
     7381 ttctttttat ataatagagt aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa
     7441 aggagagaaa agtatggcta aaaaaacaat catgcttgta tgttcggctg gtatgagcac
     7501 aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca aaaggaatcg actctgatat
     7561 tttcgctgtg tccgcttctg acgcagataa caatcttgca aataaagacg tggatgtgtt
     7621 gttattaggc ccacaagttc gctttatgaa agctgatttt gagaaacgtc tggaaccaaa
     7681 aggcattcct ttagatgtca tcaatatggc tgattatggt atgatgaatg gcgaaaaagt
     7741 gttagatcaa gcaatcacgc tcataggtga ataaatagaa cagtataact atcgaacaag
     7801 cgatccaaaa aatagatcgc ttgttttttc gtatattttt ttagggcgaa taatatgaat
     7861 catttttggg gtagaaggct tctgacgaga gatattaaag caatagactg tgaggaggta
     7921 aatttaccaa attatgagaa gatcgcagca gtggaacgtt tgagcaactg gattctttgt
     7981 catcgctgtg gaatgagaac gtccgtcatt gatggacagc ttgcagatta cggttatttt
     8041 tgtacacatt gtctatattt agggcgatgc gacactcaac aagatttata tttgtttgat
     8101 cagcctgaag caaaacctcg agaagtcgtc tttttttgga caggacagct tactgaaaaa
     8161 caaacggaaa tctcaaagag aatactttgc cattcagaaa aaagacatca tcttatttgg
     8221 gctgtaacag gtgcagggaa aacagagatg ctttatccta ttttagtgga aactttaaaa
     8281 gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg tttgtaatga attgttttta
     8341 cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc tccatgggaa tacagcaacg
     8401 gcttatacct ttacttcttt tgttatctgt acgattcatc agctataccg tttttatcgg
     8461 tcctttgatt taatagtgat cgatgagatc gatgcgtttc cttataacgg ggatgcagga
     8521 ttagctcatg ctgtgcaaac agcgattaaa ccagacggac gattcatcta cttaagtgcc
     8581 acacctgata aaaagttact aaaggagatc aagcagacgt ttgacatcca caaacttgct
     8641 ttacgctttc atcggcgttt gcttcctgaa cccgtcttgg ttttcatgaa taattggcga
     8701 caaaaatgca agaacaagaa gaagataaaa tctttggtgc ggatcattca tcaattaatt
     8761 aaagaaaacc atatcctttt attttgtcca agtatcacga tgatgcatcg tttgaaagcc
     8821 acacttcaag aggctcttcc gagatatgag atcacagatg tttcttcaag agacaatcag
     8881 cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg acatactgtt aaccacgatg
     8941 atccttgaac gtggagtcac atttgaaaga atatccgtta tcgttttagg agcggatcat
     9001 ccggttttta ctaaatcttc tttggtacaa atcgcaggaa gagccgatcg taaagggcct
     9061 tatacgaaca gtcaagtata ttttttctat gaagaaaaaa ctgcagctat tcgaaaagct
     9121 tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga tactgtgagg tgcagttgct
     9181 gcagtaaaga tatcgtccaa aatttaacat tcagcgagat tattggtgtt tcgaaataca
     9241 gattacctca gacttgttca gtttgtcgcg cacaatttca tttattggaa acaagaggct
     9301 gttctggctg tcaaaaacca atccatttca atctagagag tacgaaaaag aaagtaaatt
     9361 ctatccccgt ttactgcagt gactgtcaag aatggaagaa gaaatatccg acctatgatt
     9421 ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa ggcctggctc aatcaataca
     9481 agtttatggg agatatccga ctagcaggca cctttgcata taaatggacg cagataaaaa
     9541 aagaataccc gacatacatc tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa
     9601 gaggattcaa tcaagtaagc gagatgttga aagcagcaag aattcctttt cagatgctac
     9661 tcaaaagaga gaagcatctg ctggctcaag caaaaaacac gcgaaaggaa agattggaga
     9721 tgcctcaacc ttttgaactt gcagtcacct tttctaagat acaaaataaa aaaatattga
     9781 ttattgatga tgtctataca acaggacaga ctcttttcca cgcagcagat tgtttgcaga
     9841 tcgcacagcc gaaaatcatc cgaactattt ctttggcgag atgaattaga tgtggaaatt
     9901 tgatagcgat ttaatttgca aataacctag agttcgatat aatagaatta agaagaggga
     9961 aagagtggtc cttctccttc ttgattgtgg tagacgaaag gggtaatttt tatgtttaga
    10021 tataatgtac gcggcgaaaa tatcgaagta actgaagcga ttcgcgacta cgttgagaaa
    10081 aaagtaggca aactagaacg ctatttcagt gacgcacccg atgcaacggc gcatgtgaac
    10141 ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa cgatcccatt accttatctt
    10201 gtattacgtg ccgaagaaac ttctccagat ttatacgcaa gtatcgattt ggttgttgac
    10261 aaattggaac gccaaatccg caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg
    10321 ggcatgaata ctgcaaatgc tgctgcttta ttcaatgaag aggacgaaaa cgaaaatgat
    10381 tctgaattag atatcgtacg tacgaaacgc ctttcattga aaccaatgga tagtgaagaa
    10441 gcagtcttgc aaatgaacat gctgggacat aacttcttta tttttgaaga tgcagaaaca
    10501 aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat acggactgat tgaaacagac
    10561 tgatcagcct tacttaaata acaacgaaaa cttgcagcag ctactgacag ctgccgcaag
    10621 tttttttgag tttcctcctc ttttagatga aatatgaata aattatgaag ttcttacttt
    10681 aatacgctta agatatttca attatctata actaatgata aaatgaaaag taaagtctac
    10741 atagtctgga taatacacag aaggaaaagg agattacaca acgcatggcc aatttgattc
    10801 gttcacttat cgaaaacgat aagaaagaac taaaacgttt agatagcatt gcaaaaaaag
    10861 ttgaagcata cgctgaccaa atggcagcat taactgatga acaattacag gcaaaaactg
    10921 ctgaattcaa gggcagatac caaaaaggag aaacattaga tcaattactt ccggaagctt
    10981 ttgcagtcgt ccgagaagca gcaaaacgtg tattaggttt atatccatac cacgtacaat
    11041 tgatgggtgg aatcgtgctt cacgatggaa atatccctga aatgcgtact ggtgaaggta
    11101 aaaccttgac cgcaacgatg ccagtatatc taaatgctct gactggcgaa ggggtacacg
    11161 tagttaccgt caatgaatat ttagcaaccc gtgactcgac ggaaatgggc gaactttata
    11221 atttcttagg attaacagtg gggttgaata tcaactcaaa atcttcggat gaaaaacgcg
    11281 aggcttataa ttgtgatatc acttatagta cgaataatga attaggattt gactacttga
    11341 gagacaatat ggtcgtttac cgccatcaaa tggtacaacg tccgctgaat tatgccattg
    11401 tcgatgaagt tgactcaatc ttgattgatg aagcgagaac gccgctgatc atttcagggc
    11461 aagcagaaaa atccacagct ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag
    11521 aagaagaaga ttataaaatc gatgtacaat caaaaacaat cagtttaacc gaagcaggga
    11581 tcgaaaaagc agaagaaaca ttcggtttgg ataacttata cgacattgaa aatactgcat
    11641 tgacccatca cttggatcaa gcattgagag ccaactatat catgctttta gatatcgatt
    11701 atgttgtcca agacggaaaa gtactgattg ttgaccagtt cactggtcgg atcatggatg
    11761 gacgccgtta ttcagatgga cttcaccaag caatcgaagc taaagaaggc gtagagatcg
    11821 aagacgaaac gaaaaccatg gcgacgatca ctttccagaa ctatttccgt atgtacaaga
    11881 aacttgcggg aatgactggt acagctaaaa cagaagaaga agaattcaga gaaatttata
    11941 acatccaagt ataccagatt ccgacaaatc gtccggttat ccgtgatgat cgtccagatt
    12001 tgctttaccc aacattacaa agcaagttcc acgctgtcgt gcaagatatc aaagatcgct
    12061 accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt tgaaacatct gagttattat
    12121 cagatatgct gaatcaagaa cgtattcctc atgaagtgct gaatgcgaaa aatcacttta
    12181 aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca
    12241 tggctggtcg gggaacagat atcaagttag gacttggggt aagagaactt ggtggtctag
    12301 cagttatcgg gacagaacga catgaatctc gtcgtatcga taaccagctt cgaggacgtg
    12361 caggacgtca aggagaccct ggtatgtcac agttctactt gtcattggaa gatgatttga
    12421 tgaaacgatt cggttcagaa cgaatcaaat ctttcttaga tcggatgaag atcggtgagg
    12481 aagatgctgt tatccaaagc aagatgctga cgaaacaagt agaatctgca caaaaacgtg
    12541 tagaaggaaa taactatgac acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg
    12601 aacaacgtga agtaatctat aaacagcgac aagaagtaat catggaagaa aaaagcttat
    12661 ccaagccact aatgaacatg gtcaaacgga caatcagtcg tgtagtagat gcgcatacac
    12721 aattagagaa agaaaattgg aatttggaaa gtatcatcga ttttgccggc agtactcttg
    12781 tccatgaaga tagtattact cttgcagata tcgaaggaaa aacagctcag gagatcaaag
    12841 agtatctaaa cgaacgagca caagaagtat ttaataccaa agcggctcaa ctaaatggtc
    12901 cagagcaact gttggaattt gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga
    12961 cagaccatat tgatgcaatg gatcaattac gtcagtcaat cggcttacgt gcttatggac
    13021 agaacaatcc attagtcgaa tatcaaacag aaggctataa gatgtttgaa gatatggtag
    13081 gcgccatcga gtatgaagtc actcgaatct ttatgaaagc tgagatccgt caaaacgtac
    13141 agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga agacggagat gccaaagaaa
    13201 atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa caaggtcgga agaaatgatc
    13261 cttgtccttg tggaagcggg aaaaaatata agaactgtca tggaaaaaat gcaaactaat
    13321 ttctgacagc aggattggtg agacaaattc tttttttaag aattcgtctc accttctttc
    13381 tgttatttgc tgattctgtt agactagata taatgaaaaa agtatagtga aagaaggaaa
    13441 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
    13501 cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa gcagaagcaa
    13561 aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa
    13621 acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa ctggaagaaa
    13681 tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agccatgcaa gaagaagcag
    13741 aaacacgtat caatgcgcta gatgaaaaaa tgaagacata tgaattatct atgttgttag
    13801 atggtcctta tgatcgaaac aacgccatcg ttgaattgca tccaggggct ggcggaacgg
    13861 agtcccaaga ttggggcagt atgcttttac gtatgtatac acgctgggca gagcagcatg
    13921 ggtatcaagt agagacacta gattatcaag caggtgatga agcaggaatc aagagtgtga
    13981 ccctactgat caaaggacac aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc
    14041 gccttgtacg tatctcgcct tttgactcag cgaaacgacg tcatacttct ttttgttcgg
    14101 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
    14161 aaatcgatac gtaccgagca agtggtgcag gtggtcagca catcaataaa acagaatctg
    14221 ctgtacggat cactcacttg cctacaggaa cagtagtagc tagtcaggca caacgttcac
    14281 aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc taagctttat caattagaag
    14341 tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga acaaaaagaa atcggctggg
    14401 gttcacagat ccgttcttat gttttccatc catattctat ggtaaaagac catcggacaa
    14461 acgtagagac aggaaatgtc caagcagtta tggacggtga ccttgattta tttattgacg
    14521 gctatttaaa aatgcatcta caataagaac ccattgttaa attgaaataa aattgtaaga
    14581 ttttgcaagc atgttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
    14641 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc
    14701 tatccgcaat ctctcaattg tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc
    14761 tggagccggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
    14821 aactttgaac gtcgcaggtc atgatctaat agcaatcaaa aaccgcgaag ttccttatct
    14881 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
    14941 tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg
    15001 agtaatggaa gtgctggacc ttgttggttt gaaacacaaa gttcgagtct ttcctagcga
    15061 attatccggt ggggaacagc aacgtgtctc tattgctcgg gcaatagtga atacgcctaa
    15121 agtacttatc gcggatgaac cgacaggaaa tctggatcca gaaaactcat gggaaatcat
    15181 gaaattatta gatcggatca atgcccaagg aactactgtc gtgatggcta cacataacag
    15241 tacgatcgta aatacgattc ggcaccgagt aattgctatc gaaaacggcc gcattattcg
    15301 agatcaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
    15361 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa
    15421 cgatcacatt gactttagtc ggtgtattca tggcagttat catgaacgca actaaattag
    15481 ctcaagatat agaaggaaac gttgatgtct ctgtattcgt agatatcggt acaaaagacg
    15541 aagagatgaa aaagctagaa aaagaattga atgacttaga tcatgttaaa aaagttgaat
    15601 tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat
    15661 tgtttgaagg tgacaataac ccgttatatg atgtgtatgt agtaagtgct actgatcctt
    15721 cttatacaaa acaagtagca gaagaagcag cagatttgaa gaatgtttct cgttctgatt
    15781 acggtggagc ttcctcagat cggattttcc aaatcgctgg tgcagttcgg acatggggat
    15841 tagttgcagc ggcattgctg ctgttcgtag cgatgtttct catttcgaac acgattcgga
    15901 tcacgatttt gtctagacag cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg
    15961 gatatattcg ctggccgttt ttccttgaag gtggctggat cggtctgatc ggtgcgattc
    16021 ttccgatttt gatcatcaca tttggttatg catggttttt ccaattgatc aatccatcat
    16081 tattacgttc ccattattca ttgattcatc cacaaaatat cgtttggaaa atcaatcttt
    16141 taatggtcgg cattggtgtg atcattggtt cacttggctc gatcatttct atgagacgtt
    16201 tcttgaaatt ctaataccac aatataaata aaatgagaaa gttggacagt ttcgttccag
    16261 ctttcttttt ttattggcta ggagaataga aggaagcggc ttttttccct ttactttaaa
    16321 aaacaacttg ggtattctta agttggtaga agaagaggtg aagccgtgaa aattatcgtt
    16381 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat catcgttttt
    16441 cgagaaagaa aggaaactgc tcaaacatgg gcatgggtgc tcgttttgat gttcattcca
    16501 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
    16561 gatttgaaga tccaagggaa aatcggtaag aacctggaaa ttgaagaaga caaacaggca
    16621 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
    16681 tatatgctca cggtatttga aagtacgctc tatacgacta aaaatgaaat cactttattt
    16741 acagatggtc gtgaaaaatt cgatgcgctg attgaagata tcaagcaggc agaagaccat
    16801 atccacttcc agtattatat ttatcgcagt gatgcattag gtgaagaagt tcgagatgca
    16861 ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc
    16921 acacaagttt cttccagctt ttttcagaat ttgaaaaaag caggcggaga agtcgcattt
    16981 ttctttcctc tatttgttcc gtatattaat cctcggatca attatcgcaa tcatcgtaaa
    17041 atcgtagtta ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaatgaatat
    17101 ctaggattgg taaagaaatt tggttattgg cgagacaatc atttgcgtat ttacggagaa
    17161 gcggtgtata ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
    17221 gtaggctatt ctcccaaatt ttttccgtcc attcactcta ctggtgatat agctgttcaa
    17281 atcgtgacaa gcggaccaga taccgaacat gaacaaatca aaatgactta tttgaaaatg
    17341 atttcgttag ccaaacggga gatattgatc caaacgcctt attacatacc ggatggatcg
    17401 attcatgaag cactgaagtt ggctcttctt tcaggcgtaa aagttcatat ccaaatacct
    17461 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctaatc
    17521 gagtatggag caaggatcga aacatatgag aatggattta ttcatgcaaa gacgatgatt
    17581 atcgatggtg gcattgtttc agttggctca gcaaatatcg atgttcgttc atttcgcttg
    17641 gactttgagg tcaatacact ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct
    17701 ttttttgaag actcgaaaat ttctacgcat ctcacaaaag aaatgtatga aaaccgagga
    17761 ataatcatca agatgaaaga aggattagca agattgattt cgccgctcct ttaaaacata
    17821 aaaaggacat gagaaccatc aacagatgga gatctcgtgt ccttttctaa cggggccatt
    17881 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
    17941 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
    18001 ttcaaagcca gtatagcata gaaactttta atgagatagt tttttttaca attatga
//
LOCUS       DAYYAJ010000058        17963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000058 DAYYAJ010000000
VERSION     DAYYAJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.058"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1391
                     /locus_tag="ACHATZ_001595"
     CDS             51..1391
                     /locus_tag="ACHATZ_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8404121.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1586..2029
                     /locus_tag="ACHATZ_001596"
     CDS             1586..2029
                     /locus_tag="ACHATZ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8404122.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2291..3709)
                     /gene="nhaC"
                     /locus_tag="ACHATZ_001597"
     CDS             complement(2291..3709)
                     /gene="nhaC"
                     /locus_tag="ACHATZ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8404123.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4113..5336)
                     /locus_tag="ACHATZ_001598"
     CDS             complement(4113..5336)
                     /locus_tag="ACHATZ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8404124.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5368..6207)
                     /locus_tag="ACHATZ_001599"
     CDS             complement(5368..6207)
                     /locus_tag="ACHATZ_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8404125.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6224..6904)
                     /locus_tag="ACHATZ_001600"
     CDS             complement(6224..6904)
                     /locus_tag="ACHATZ_001600"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8404126.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6906..7979)
                     /locus_tag="ACHATZ_001601"
     CDS             complement(6906..7979)
                     /locus_tag="ACHATZ_001601"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8404127.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(8038..8276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8319..8552)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8629..9300)
                     /locus_tag="ACHATZ_001602"
     CDS             complement(8629..9300)
                     /locus_tag="ACHATZ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8404128.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9388..12036)
                     /gene="mgtA"
                     /locus_tag="ACHATZ_001603"
     CDS             complement(9388..12036)
                     /gene="mgtA"
                     /locus_tag="ACHATZ_001603"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8404129.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWF
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12379..12553)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12726..13163
                     /gene="ndk"
                     /locus_tag="ACHATZ_001604"
     CDS             12726..13163
                     /gene="ndk"
                     /locus_tag="ACHATZ_001604"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8404130.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13185..14051)
                     /locus_tag="ACHATZ_001605"
     CDS             complement(13185..14051)
                     /locus_tag="ACHATZ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404131.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14285..16363)
                     /locus_tag="ACHATZ_001606"
     CDS             complement(14285..16363)
                     /locus_tag="ACHATZ_001606"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8404132.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16856..>17963
                     /locus_tag="ACHATZ_001607"
     CDS             16856..>17963
                     /locus_tag="ACHATZ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8404133.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         5237 a         3766 c         3173 g         5787 t
ORIGIN      
        1 ttattcttac tatgcatttt gattgtgaat ttaaaagaaa gaggttattt atggaagata
       61 aaaaattgaa aaaacaactt agctcccgac atatcaccat gctagcttta ggtggagcga
      121 ttggtgcagg tctatttaaa ggaagtggcg aagcgattgg aatcgccggc ccttctgtat
      181 tgatcgcttt tttgattggc ggcataattt tatatatcgt gatgaaaggt ctgggaaaaa
      241 ttgttttaag tggtggtgat acgcaccacg gattatctgg attgattcgt ccatatctag
      301 gtgctcatgc tgccgatttt accgattggg tctactggtc catgtggatt atcaatatca
      361 ttgctgaagc tgttgcagct gcttcctttt tacagttgtg gttcccccac atcccagctt
      421 gggtatttgt ttttatgttg gctgttttaa ctactatcat caatctttat tcggtacgat
      481 tattcgcaga gacagaatat tggctggcct ttgctaaaat cagtgtcatt atcctattga
      541 tcatttttgc tgcttatctt gtcggtcagc aaatgttggg tactggtgtt ttccctaccc
      601 ttcaacaatt aactgatcat ggtggtttta ctccccacgg tatgaaggga atcatcagtt
      661 ctttgcttgt agttatctat tcttacggcg gttctgagtt gattgctatc acagtaagcg
      721 aagcagatga tcctaaacgt gcgattccta aagcaatcaa aggcgtcatg ggaagaatca
      781 tttcctttta cattattcct ttatttttgc ttttgattat ttttccttgg aatactttag
      841 caggcacgaa cgttagtcct tttgtgatgg tatttgaaaa aatgaatatt ccatttgcgg
      901 cagatatcgt caatttcgtg attgttcttg ctctgttctc gtctatcaat tcaggtgtat
      961 atgcttcttc gagaattttg ttcttccgct tgaaagatcg gaaaggttcc tctagaaaac
     1021 tagctgtttt aaacaagcat caagtaccgc aacgtgctgt gctgttttgt accagtgttc
     1081 tttatctagg tgtggctctt tcttattttg tcggtgacaa attatttggc tatctggcag
     1141 gttctctttc ctatacagta ttattgatct ggatcattat cagtgctgcg tcctttgtac
     1201 tgacactaag gaaaggaacg ttatgggaca aaggaatcag cttttttgca ttagcagtct
     1261 tatggttgat tttccttggt atcctattca cgaactccat tggggtaact gtattgaccg
     1321 gtttgcttta cctgttcatt tatttcagtt atcaaaaaaa gaatgatgct ttcgtcttga
     1381 cgaatgaatg aaaccaatag agaattttat tctatttata ttgaatatct ggagctattg
     1441 aaaaaaagga gctgcgacaa aaattgttgc agttcttttt tccgaataaa cggtgttcaa
     1501 aaaccatctg tgcagattaa ttgattgttc tccacgctca tgacatgata gaagaaagga
     1561 atgaagaaaa aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgattgg
     1621 tgtttcgacg gtagtcatgt ttggtatgat gtatttgaat gtttatgcgc ttgatcatat
     1681 cttcttcagt cgcacacgtg tattcatggc gttgatgatg ggtgcagtca tggcagtcat
     1741 catgttgttg ttcatgtgga aaatgtatga aaacaaaaag ataaatgcta tgattttagc
     1801 cgctagtctt cttgtttttg ccggttcctt atttatggta cgaagccaaa caactgtcaa
     1861 cgatacggcg tggatgaagg cgatgatccc tcatcactct attgctatct tgacaagtaa
     1921 acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca
     1981 agaagaagaa atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccat
     2041 ccaaataaaa aaagaggatg ttccagaagt gatcaacttc aagaaataag aagaagctac
     2101 ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     2161 agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac cgcaaatgca
     2221 agcttttgaa caccgtttat tcgtaaaagg gagtgtgaca aaagtcttgt cacactccct
     2281 ttttacgttt ttattgttct aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa
     2341 atttctttga gatcccccaa cctgtaaagg cactgaccat cgaaaacaca ggtgaaagca
     2401 aactgaaaaa cacgaatggc aagtaagtga gtgtaggaat gccaaatgta cttgctacga
     2461 aacttccggc gattccccaa ggcactaagt agttgattac tgttccacca tcttctagtg
     2521 ttctccctaa tacagttgga tcaaatcctg ctttttcgta agtttcttta aatgcattac
     2581 caggcaggat aacagataga aattgttctc caataaagat atttactccg atgcttgtca
     2641 tcaatgttgt aaaaatcaat tttggggtgt tcgtcatctt tcttgatact cgttccataa
     2701 cggttgagat caacccaaat tcgatcaaca aaccacctaa tgacaatgtt agaataatca
     2761 atgacacagt tggcatcatg ctttcgattc ctccacggct aagtagtgaa tcgatttgtt
     2821 ggtttccagt atttgaaaca aatcccgatt cgatgacccc tgccaacttc gttaaggaag
     2881 tttgtgggtt ttgaataaag atcattccta ctgctacagc aatattcaac aaaatcgtca
     2941 aaattgccgg tacctttttc catgcacaca agagcattaa aaatagagga atcagtgacc
     3001 agatcgaaat agtaaagttt gcttctaaaa tatcagtcac ttcacgaata gatgaaagac
     3061 ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg
     3121 gaacagttga ccacatcata tgcttgatgt gtttgaacaa atcggcatca acaatagctg
     3181 ctgctaaatt tgttgattcg gataatggag acatcttgtc tccaaaaacg gcacctgaaa
     3241 caatggctcc aacaacaagg gctggattta gtcctagtgt tgtcccaata ccgaaaaatg
     3301 caatcccaat cgttgacata acagtaaagg cactccctac agcgctacct acgattgcac
     3361 aaacaacaaa tacagaaggg acgaaccact ggacactaat caatttaaat ccaatgacca
     3421 tgagggttgg aataattcct gcttgtatcc atacagcaat caatgaaccg actaaaataa
     3481 aaataaagat tggaatgatc cccggtttga tgccattgat gatcccttcg tgcagccaat
     3541 ctgtcgggta tttttttatc actgcgtaca acatgaccat tccgatagct gccaaaatcg
     3601 tgatattagg tgaaaccttt aatccgacaa cgccaatcgc aatgcctaaa acaatgacca
     3661 tcaatacaag tgttgcttct aaaaaacttg gtgttccttt tttttccatg ataatctctc
     3721 cttttttagt tgttcttatg ttctaaaaaa gttaagatat cggattactc cgccacaatt
     3781 catccgcgat tcctccttta taaaaataaa aagacctgct ttcaactaag gacgaatgac
     3841 tccgcggtac caccttaatt aaaaacagat ctgtttttca ctttcaaacg attattcaaa
     3901 tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac
     3961 tttctgaaca aaacacctaa ccactactgc gtgaaaagtt gacttgtatg aagtttacct
     4021 tccataagat catttgtcaa tgaaaaagaa aatatttttt catttttctg aaaattattt
     4081 ttataacttt cgcgcacaac tttatctttt tactagaaag tagcgccctc ttgttttttt
     4141 cttgctacta tttcttgatg ttcttgtttg attttttcta atagcttggg ttctctcaat
     4201 aaatctagag ctgtattggc aagtaattcc gctgcaatcg caatagaagc aagtcctttt
     4261 tcacttttag cagcagcttt gaattcttct gtatgtccgg caatatatgt ctcagagatt
     4321 gctacagtag gctgtatcgt tggaataact tggctaacat ttcctacatc agaagaacct
     4381 agacttgttc tttgttccgt ttctatttct tcatcaggaa cccctgcttc ttttgcatgt
     4441 tggaagaata agtcatcgaa agaaggcgtt acgatgacat catcgactgc attttggaac
     4501 aatccaaact caaaagtcgt acccgtgctt aatgcagcac ctcgtacgat attttccact
     4561 ttttcatata ctttgtctaa tgtttgtcga tttcctgcac gtaaatagaa acgtccagca
     4621 gcgtagtctg gtatgacatt ggccgccacg cctccatctg taatgattcc atggatattg
     4681 acatcttttg gcaattgcaa gcgtagagcg ttgattccgt taaacacttg gatcaaggct
     4741 tccaaagcat ttactccttt ttcaggtgct gctgctgcat gagaagcgac accgtaaaat
     4801 ttgatatcga ctggatcatt tgctaaagat tctgtcgttt tcccataacg aaaagctgga
     4861 tggacacata atgctgcatc gacatcctca aaaaaacctt cccgaacgaa gcttccctta
     4921 gctgatccat tttcgcctcc ttcttccccc ggagtaccat acacacgaat ttctccgcca
     4981 atctcatcaa tgaattgttt cactatgctg gctgctaata cggagtaagt accaaataaa
     5041 ttatgtccgc atgcatgacc aatacctggt aaagcatcaa attctgccaa aaaggcaatt
     5101 gtcggacctg gtttattgga aatataacgt gcatcaaagc ctgttcggtg cccagctact
     5161 tctttttcta cccagaaccc ttctttttcc aattgtttga ttaaagctgc tgaggaataa
     5221 aattcataat tacttacttc aggatggtcg tggatagcta gtgcaagtgc ttgatattct
     5281 ggtaaacggt attgaatcag atccttcaat tgatttgttg agagtgtggt tgtcataaaa
     5341 atttcctcct atgctagtcg ttttttctta ttttctcccg aatgtttccc acgcaggtac
     5401 gcttgaacct tttgatgttt tttcgattac tttggctgtt tcttctgttt gataggcatc
     5461 tactattttt tgatacgttt tattgtcttc atcttctttt cttgcaacga tgatattgac
     5521 ataaggacgt gcatgctcat cgactggttc taagaagatt gcatcagaag cgggattaaa
     5581 tccagcatct acagctacac cgctattgat gactgaaata tctacatctg acaaagcacg
     5641 tgctgtttgg gaagcatcta actcagaaat gtctagattt tttttattct cggtaatatc
     5701 agaaacggta ggcgcttggc cttttgcctt gtccactttg atcaatccag cagtttgcaa
     5761 taacagaaga gctctgccgc cattcgtgac atcattaggg attgcaactt ttgctccatc
     5821 tttgatatca gccacatctt ttaacgtatt ggaataaatt cccaacggtg cattaaccgt
     5881 attcccaatc gatactaaat cagttccgtg ttcttcattg taattgtcta aaaagaattg
     5941 atgttggaag gaattcaaat cgatcgatcc atctgctaaa gccgcatttg gctgattgta
     6001 atctgtaaat ttcacatatt ccaaatggat gttttccttt ttcaaacggt ctttcacatc
     6061 gtcccataca tctgtatctt ccccaatgat ccctagtttt acggtcgttt cttttttttc
     6121 agttgtagct gcttttcctt ggctgcatcc tgccaaccaa ataattgcta caaatgtaat
     6181 gactgcaaaa cgaataattt ttttcatttt ttctcctcca ttttcaatgt gtgacttttt
     6241 taatcagttg attactgata aattgactca aaaaaactaa gaccagaatc aataatgtag
     6301 cgacaatcgt tacatctgtt tggaatcgat tatatcctct cgatatagca agattcccta
     6361 atcctccggc acctactgct ccagccatgg tagtcagtcc gatcacattg atgatcgtca
     6421 acgctgagac ccgaatgata ccaggtaacc cttctaacaa ataaacacgt aaaatgatgc
     6481 cgattgggct cgtgcccatc gcttctgctg cttcgatcac cccaggatca acttctagca
     6541 gtgcattctc gatttgacgg gcaaaaaacg ggacaacgcc aaaaatcaaa gggactaaag
     6601 ctgctttggc accaatcgtc gttccaacta agaaacgagt agtcaaagct aaaagagaaa
     6661 gcaagataat gaaagggatg gaacgaatga tattgatcaa cttatcaagt atttcgaaca
     6721 gcagcctgtt agctagtaat ccatttggac gtgtcactac taacgtcaca cctaagatga
     6781 ttccgatgat cccaccaatg aatgccgtcc aaaaagacat atatagtgtt tgctttgtcg
     6841 cttcgatgaa ttcatccgga atcaaataga cattcggcaa atatgtttta aaaaattcag
     6901 tcattttagt ttcctccttc gatgactttg atacggttat ctaattcatt tttttgtaaa
     6961 atagttactt ttacaccaga tttaatcaaa taatccaatg ctttttgccg ctgtttggtt
     7021 tctccagcta aagtaacgat caaacttcct aagggtgttt cttgtaatag ctctacatta
     7081 ccgtataaga tattcgctgt tacttggtac ttgctgtaaa gatgagcgat tagaggttcg
     7141 ttcgtctggt tgccgatata acttaattcc accaaccatt tatttgcaat ctgatcggca
     7201 aaagcagcat gggcactgat tgtttccaat gcttggtcaa ggtgtgtcgc tgtccgaata
     7261 aaatctttag tcaattcttc ttcaggatga ctgaaaatct ggatgctgct acctttttca
     7321 atgattttcc ccgcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc
     7381 tcatgagtga tcaggacgat tgttagacct agctgacgat ttagtttttt taacagtgca
     7441 aggatctgaa gggtcgtttt cggatcaagt gcactagttg cttcatcgca taacaacact
     7501 ttaggttcat ttgccagagc tcgtgcgatt gccactcgtt gtttttgccc ccccgacagt
     7561 tgtttaggat acgcagatgc tttttcttcc agaccgacaa gttccagtag ttcattcact
     7621 ttttgacgac gttcctgttt tgattttcca gaatacttta gagaaaaatc tacgttgtca
     7681 aaaatcgttc gactttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt
     7741 tcttttcgca attcttttgg agagagctgg cttaaattcg tctgattgac gatgacttct
     7801 cctgctgttg gtttctgcaa caaattcatc acgcggacta acgtactctt tcctgcacct
     7861 gagtaaccga cgatcccata aacatcccct ttttcaatcg ttaaatctac agaatctact
     7921 gcctgtatct ctttttcttt ttgtttgaaa catacagaaa tatctcgcaa ttcaatcatt
     7981 aacattcctc cttgtctata aaaaaagcat cctcaagaaa atgaaaatag tcattttctt
     8041 gaggacgcct ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc
     8101 ttcaagtacc atcatactct agcaagatat cggtcgcagc cgtcaccgcc ttacatctat
     8161 gctcgggatg aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct
     8221 accgaagctc tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt
     8281 caaaaaaaaa atccttttac taacataaac tatgtcagta aaaggacgac gtatcgtggt
     8341 accacctttt ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc
     8401 gttcgtttta acggtcgaac atttccgaat ttgcctactt atcgacaaat tattctccaa
     8461 ggccattttc agcttttctt acttgactct tcacaccagc cagagcctct ctgcaaagtt
     8521 tccaagctta ctttccttct caacgaaatc aatattttgt tgttgggaat aatctacagg
     8581 aggaaaactg aaaagtcaag catgaaatcc aaattatttt ttctatattt atttttctgt
     8641 ttttttatct aacatttcaa taacagttcg aagatctttt ccttccgcaa gaaggttcaa
     8701 tttttccttc tcttcgacgc tataatttcg aacaaaactg gaatcgggaa tcaaaacaac
     8761 gcctagtcct gcacgtgaag cggctgtcgc tcctactaaa gaatcttcca ctgcaaaagc
     8821 tgaaagagct tctaatcctg ttcgttccaa agctgtcaga taagggactg gtgaaggctt
     8881 gttctccgat acttcatctc cgaacgtaaa cgtatcaaag taatcggcta attgaaagtg
     8941 atccaagata tctgttgctc ttttcgtaac agttgtcgta gcaatcccta gtttcatatg
     9001 acgttctttt aagtctgtca atatttcttt tgcatagggt ttcaattgga tagctccact
     9061 tgtcagctgt tgataaaaat attcttcacg ctttgctcgg acttcctgca ctttttcagg
     9121 actgcctgct gcctgcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc
     9181 gtcaacgatt tctttcggga tatgaatatc aaattgctgg aatccccaaa gccaaccatc
     9241 ccgatacgtt ctttccgtat ccactaacaa accatctaaa tcaaatattg cacctttcat
     9301 ctatacatta atcctttcca aaacaataca cctagtttag catgactgat ccaaaatgaa
     9361 cagaagcata gagcccagcc gcatcactta taagagttct tggaatcgac ggatatagat
     9421 tttcttcaca gctgtcacaa gcgccagata agcgacgact gttataaaaa accataacca
     9481 gtaattgcca gggagcggca tcaagcctaa ctcagcacca aatcctgtga atggaaggac
     9541 tgaaccgaca atgattccta atgtagtgat aaccagcatg gcaaatgttg cattgctttg
     9601 gataaatggt attttcggtg tacgcaatgc atggagtact aatgtttgtg tccacaggga
     9661 ttcgacaaac catcctgcat ggaataatgc gataaaaatc acttgttgtg agctatccaa
     9721 tgtatggaaa cttccaccaa ctacagcagg acaaataaca aaatacatca acaagtaagt
     9781 agtgatatca aaaatcgaac ttgttggacc aaaataaacc atgaattttc caatactaga
     9841 tgcattccat ttctttggct catccaaata ttctttatcc atccgatccc atggtaagga
     9901 aatgcaagaa acgtcataga tcagattcaa aaatagaagt tgcaaaggca acattggtaa
     9961 aaatggtaag aacgtactgg caatcagtac ggaaaacata ttgccaaaat tagaacttgc
    10021 tgttgccttg atatatttca tgatgtttcc aaaagtttct cttccagaaa gaaggcctct
    10081 ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct gctgattctt ttgcgatatc
    10141 cacagctgta tccacggaaa taccgatatc agccactttc attgctggag catcattgat
    10201 accatctcct aaaaatccga cagtatgccc atttttacgt aaagctgtcg tcaatcgtgc
    10261 tttttgctga ggattcaatt ttacgaatac ttcgtgttct tcagctactt gcgctaattc
    10321 atgatcattc atttccgaga ttttctcacc agtaatcaat tcattgattt ctaggcctac
    10381 ttgacggcac acagaccgtg tgactaattc attgtcccca gtcagaactt ttacagctac
    10441 accatgttct tttaaagctt tcaacgcttg ttttgttgtt tcttttggtg gatcaaggaa
    10501 agcaagataa ccaatcagaa ccatgtctga ctcatctttg actgaaaatt ctccaacaac
    10561 gcttggattg gtcttttgtg cgacaccaat cactcgtaag ccgtcttcat taagtgattg
    10621 tacttttgca agcaccgctt cttttcgttc ttttgttaat ggtgcgacag taccaccaag
    10681 atcaataaat ttagaaatgt tcaacatttc ttcaatcgcg ccttttgtga tcatctgtgt
    10741 tttgccagcc ggatcagcga ctacaacact catacggcga cgttcaaaat caaaaggaat
    10801 ttcatctact ttttgataat tgattgtatc tgtatctaaa gtttctgctg cttcatcaat
    10861 gatagccaca tctaacaaat ttttcagccc tgtttgatag tagctgttta gataagcatg
    10921 gcgcaaaaca cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt
    10981 gtcttgtgtc aatgttcccg ttttatccgt acataacaca tcgatcgcac caaaattctg
    11041 gatagcattt aaatttttga tgactgttcc ttttttggcc atcacacttg ctcctttgac
    11101 caaattcgtc gtaacgatca ttggaagcat ttcaggcgtt aaaccaactg ccacagataa
    11161 tccaaaaaga aaagcttcta accaatctcc ttttgtaagc ccattgataa cgataactgc
    11221 tggagccatc aatgacataa atttgatcag cagcatggat gttttgtgga tgcctaattc
    11281 aaaacttgat tggatgggtt tttcagatag cgtttctgct acatgcccaa atagtgtgtc
    11341 atttccgaca gaaacaatga ttccttccgc gcttccactg atgacattgc ttcccataaa
    11401 gacaagattt tcataacttg tttcactttg ctgatcgtct tcacatactt tcgcatgctt
    11461 ttctactgga tagctttccc ctgtcaatgc tgcttgagaa atgaatagat cctttgcgga
    11521 caataaacgc atatccgctg gtatcatatc tcctgctgaa agccgaacaa gatctccaca
    11581 gacgatttct tccataggaa tctctgtata ctcgccattt cgtttgacag ctgccgttac
    11641 tttcacaagt gatttcagtt tttcagctgc ttgattagaa cgcacagact ggatcaacgt
    11701 catcgtccca ctgacgataa ccatagtgaa gataatggct acaccaaaga gatctttatc
    11761 tcctggagcg gctaatacgt attccgtaat gaatgaaata attcctaatg caatcaacac
    11821 taatgtaaag ggtgtgatgt atgctttcaa tatttctata atgaatggtg tttttttgcc
    11881 atatgaaatc gcattttcac cataagtttc tcttgaacgc tcgatttgtt cttctgacaa
    11941 tccatttggt gatgcatgga acaattcaaa taattcttgc ttcgtttttc cagcaaaagc
    12001 tgcatagatt ttttgtgtat ctttcttttt ttccatctcg atctctcctt tgtgtccaaa
    12061 agacagctgt tctcgctgtt tttggtcttt tgctattcat cggatcgata gagaaaatgc
    12121 tgaaaggggc tgagaacaga tagataatag ccctggcctt tcctctaaat ccttgttttt
    12181 cgaatgactg tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt
    12241 ccaatcattt ttcgaatagt tattttccca actcgctcac ctcccttttt gttttttcac
    12301 ctatgggatt tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa
    12361 agcaaatcaa aaaaagcttc tcagatctca tcgttgagtt ttgacactgt acaacgtaaa
    12421 aagcagatat ctgcttttag ctaccttatg aaaagcctta tctcgacaat tcctgttcta
    12481 cccattggcg tctctcgacg tttttgggca gtagcctatg tttgtatagg agcctcacct
    12541 aacaaatcct attcggtttt ccctcgttaa tataacaact aaaaaagagg atgtcaaatc
    12601 aattttccat gtttttcata cttttttaaa atacacgcgt tatatttcct gtctcacgca
    12661 gttttatttg tctcattttc cacctcccgc tataatagaa ttatagtagg aaacgaggtg
    12721 ctgttatgga acgaacactt gttattatca agccagacgg cgtaagacgc catttggtcg
    12781 gaagcatcat ccaacgtttt gaagcaaagg gactggccat tgctgaaatg aaatttgcaa
    12841 cgatgacacc tgaactagca aaagaacatt atcagcactt gactgaacgt agtttttttg
    12901 atgagcttat cgattacatg acttctggtc cggtcgttta tcttgtgctg gtcggcgagg
    12961 aagtgatcga tatcgtccgt aagatggtcg gagcaacaaa aggggcggat gccgttccag
    13021 gtacgatccg aggagattat gcactgccgg ggactgaaaa catcatccat gcttctgatt
    13081 cccgtgatgc tgcaacaaaa gagatcgccc gctttttccc gccattcgat acaaacaaac
    13141 cgtctgaaaa agcggcgaag tgatacaaat cctaaactgt tggtctaact gctgggttta
    13201 gcaatggcca gcagtttttt ctctaaagaa ttcctttgat tcttccctaa tgtatattcg
    13261 atcactgcga aaaactcttg gtgattttcc gatttttctt cccctaaagc agcagaaatc
    13321 agtcgttggt aataatccgc catcaatgtt tcataaatca ctgtacttct ttctttatgc
    13381 gctaaactcc gtttcatatc ttcattcacg ctacgtgcct tttttaagcg accattttct
    13441 atataaaaca aagtcaagtt cgtcaaaaaa gtaaagagat actgctcgtg attattcagt
    13501 tgcgcgatcc gtcgcattct ttttttgatt ttcaaataat tattctcaat aaaacgatca
    13561 ctgaatatcc aaggaaaatc tgctagaata gatacttcaa attgcccata ctctggtact
    13621 tgcatcaaat attcttcaat gatacgacat tcataacgca aaaattcaaa ttccgtctca
    13681 gatacttctg tttttttgga catgtgcaga ttcaaaacca aatagtacag atcttctgtt
    13741 ttcttatagt gcctgcggtt attagcaatt tctttgtaag gatcaaagct gtcttcttga
    13801 atccgatcta gcattcgtct ttttttcttt aatttttttg gctcatcaag ctgatttcgt
    13861 atccattcat attcggcaag tgtcatgttc attcgctcta aaaatgattt caatgttcca
    13921 aacgatactt ttttcactga atttgtctcg atcccgacta gcgaagtttt agaacagatt
    13981 ccttccgcta attgggtctg tgtcagcttt ttgttgactc gaagcttctt aatcaattct
    14041 ccatcataca taatagttct ccttttttaa ttgaaattaa cccttggctt ttccagaatg
    14101 cttgccaata agaggaatga ttatttcaat ttaaattttc cgcattcaat aaatagatac
    14161 gttaaaaata aaaaatacac gaaaaaaata aaaaaatgtt tttctcataa aaaataccaa
    14221 ccttcaagca tgagactgct gtctaatccc tgaaggttgg tttgattgtt tattgatcat
    14281 ttttttactg ctccgcattg gttcctgtta ctggatatag gatagtcgtt gttttattgc
    14341 cgttttttga aaagacactg gtcgaatgtt cacctttttg cttttcgatt gcttcattac
    14401 gttccgcttc gtttttatag tcatattttt tcggatcaat cgtctcaaat ccgtttggca
    14461 cataaaaccg caagagattt tcctgattca gcttatctga aagacttaac gcagtcttga
    14521 ctttttcctg atctttggag atttcctcgt cgattcctgc tgctttcgta tcgatgattt
    14581 ctcctgtctg attattgtaa gcttttcctc ctaggacggt gtatttgggc gtcacgaagt
    14641 tcccatttcg aaaagcaact acttgatcgt gctgagaaga taataagtct gtaccaaaat
    14701 gaagataatc tttatcatcg attcccaata gatgtagtaa tgtcggcaaa acatcgattt
    14761 ctccaccata ttcatgattg actgtccctt tagtgtaacc aggcatatgg atcatcaatg
    14821 gcactcgttg catctgtgcg ttgtcaaatt catcccatgt atctggatct ttaccaagag
    14881 cacttgctaa atctttatta tctgtattcg agatcccaaa atgatccccg taaatcacaa
    14941 agattgaatt ttggtaaata ccagattttt tcaaatacgt aaaaaattgt tccaatgatt
    15001 gatccagata atgggccgtt cgaacataat tatctactgt gctattgcca gtattcaaag
    15061 atggaaagtc gaaattcttg tcatccgtat aataaggcgt gtggttggtt acggaaagaa
    15121 atttcgtgta aaacggctgt tgtagatgtt caagatattg ggcagattct ctgaataaat
    15181 ctttatctaa tatcccataa cctaacgttt catcggattc atcaaaataa gatcgatcaa
    15241 agaaattgtc ataccctaaa tttttatata catgatctct attccagaaa ctagctacat
    15301 tcccatggaa aacagcactt gtatagcctt ctgtttgacc aagggtcgct ggtgctgctt
    15361 gaaagacatt atctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc
    15421 cagtttccaa catgttttca gcatcacttg tctttccctg tcctacttca tgaaagaaat
    15481 tgtcaaaact gatcgttgct tgattctgga aaatactatt taaaaacggc gtgacttctt
    15541 gtccgtctac cttcatattg atcaaaaact gttgaaaact ttctaaatga aggacaatca
    15601 cattttttcc tttagcaatc ccaaatgttt caggatttgg ctctgcataa tgtttctttg
    15661 tatagtctaa aacttcatca attccattac tgcttgcatg cgcgcgaaca ctgctagtca
    15721 ttcctgtttt gattccgtca taaatagtgt acgcatcgat tcctagatat ttgacaatat
    15781 aggaacggtc aaatgtccgc tgtagcaatt ggggacggtt ggcttcgctc aacgctaaat
    15841 ttcctgaaaa aatcaatcct gaaagacagg taacagcgat agccatcggt ttgctgacag
    15901 ggttgctttt gataggtatt ttcttgacct tcaaccaaac cagtagccca atgaacacag
    15961 cgatatccaa ccaatagatg acatcatggg gtttcatcaa agaaaaggag cttcctgaca
    16021 acccttgtga cactttcgaa aagcctaata cagatttgac tgtaatgaaa tctgtgaatt
    16081 cccgataaaa aataatattc aagtacaaca aaagcgtatt taaaatatca atgatcaaaa
    16141 gaatggctaa cgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg
    16201 tagtcgcaat cgggttgatc cacaagataa agtactggat cgtaccttcc acacctaatg
    16261 aaaaatctgc atagtaggca atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa
    16321 cgattgctaa acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct
    16381 attcacattt tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc
    16441 ttaagatttt tttattcttt ctaaagatta ttccattcat ttttcacagc ataaaatcgt
    16501 ttcggtgtaa gcggttaaaa tagtaaggag ataaaaatct tgtggtgacc gatttactct
    16561 atagtattca aaagtagttt ctcggtattc gtacaaaaaa aacatactaa gattagaggg
    16621 gaacatcatt gacgaatgat gttctcctct attttctatt tttagattat agtgaagagt
    16681 gaaagaaagt tcgaacaaag ccttgaggac tacgagtccc tatcaaaaac cgaacttctt
    16741 tactgtcatt ttagaatcag gtttacgtat gttggggcca tgagcccgtg tataggaaat
    16801 tggaatcgaa atgaagcagt aaagttcttt caacacttaa aaaggaggaa aacaaatgcc
    16861 ttacattctt gcattagatg tttcaatggg acacagctac agtgtcctat acaaagacga
    16921 cacctgctta tttgaagacg agatcgaaca cactcaaaga ggatttcatg cattactcga
    16981 agagatccag cacttgcctg aacgtccatt gattgttttt gaagcaacgg gtatttattc
    17041 cagaccagtc gaaaagttct ttcaagatca tcacttttct tactgtttgt taaacccgtt
    17101 agaagcgaaa aaacagatgg aagaaaacac cttacgcagt tggaaaacag ataaatcgga
    17161 tgcccatcgg ctcgcacaga ctcatcttaa aaatcaacgt caaccaaaag aagtacaaag
    17221 caatttttac ttagagatgc gcgatttagc ccgtttttat caagaaattg agaagaaaat
    17281 cactcgactg cgcatggatt tacacaactg ccttcagttg acttttccag agcttgaaca
    17341 gttcttttca aacaggctga ccccttacgc attgacattg atccgtcttt ttccccatcc
    17401 agattttgta ttggcatcta ctcggacaaa aataaaaaat aaactgatca acgaaacacg
    17461 ggaaaaaatc tccgcgaatc gtgccgagca aaaggcagat caaatcatcc actatgcaca
    17521 gtgtgcctat ccagctgttg aaaaagacag tattcactgt caaaaaacga tctattacgc
    17581 cgaacttctc caagatttac tcgaacaaaa aaaagcactg gcgacacaaa tgatcaaaaa
    17641 agccgaagcc tctccttgct ttttgttgta ccaaacgttc cctgggattg gcgcactcac
    17701 agccgcttta ttgctcggtg aattaggaga tatcacacga tttaaaaccc ataaacaatt
    17761 gaatgccttt gtcggaatcg acatccgacg gtatcattca ggtaagtata ctggccaaga
    17821 ccgcatcaat aagcgtggta atcccaaagc acggaaaatt attttcttca ccattcgaaa
    17881 tatgattcgc cagcaacgcg cagctcctaa tcatattgtc gattattact acaaattaaa
    17941 aaagcaacct atccctaaga aag
//
LOCUS       DAYYAJ010000059        17224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000059 DAYYAJ010000000
VERSION     DAYYAJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.059"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHATZ_001608"
     CDS             1..369
                     /locus_tag="ACHATZ_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8404134.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHATZ_001609"
     CDS             549..1490
                     /locus_tag="ACHATZ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8404135.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHATZ_001610"
     CDS             1559..2290
                     /locus_tag="ACHATZ_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8404136.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHATZ_001611"
     CDS             2434..4614
                     /locus_tag="ACHATZ_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8404137.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHATZ_001612"
     CDS             4634..5116
                     /locus_tag="ACHATZ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8404138.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHATZ_001613"
     CDS             5132..5893
                     /locus_tag="ACHATZ_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8404139.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHATZ_001614"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHATZ_001614"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8404140.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHATZ_001615"
     CDS             7665..8150
                     /locus_tag="ACHATZ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8404141.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHATZ_001616"
     CDS             8143..8826
                     /locus_tag="ACHATZ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8404142.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHATZ_001617"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHATZ_001617"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8404143.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHATZ_001618"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHATZ_001618"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8404144.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHATZ_001619"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHATZ_001619"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8404145.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHATZ_001620"
     CDS             13123..13455
                     /locus_tag="ACHATZ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8404146.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHATZ_001621"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHATZ_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8404147.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHATZ_001622"
     CDS             14281..15654
                     /locus_tag="ACHATZ_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8404148.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHATZ_001623"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHATZ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8404149.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHATZ_001624"
     CDS             16709..17149
                     /locus_tag="ACHATZ_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8404150.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5874 a         3009 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 ttaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DAYYAJ010000060        16881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000060 DAYYAJ010000000
VERSION     DAYYAJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.060"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(258..1763)
                     /locus_tag="ACHATZ_001625"
     CDS             complement(258..1763)
                     /locus_tag="ACHATZ_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8404151.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="ACHATZ_001626"
     CDS             complement(1760..2446)
                     /locus_tag="ACHATZ_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8404152.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="ACHATZ_001627"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="ACHATZ_001627"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8404153.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="ACHATZ_001628"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="ACHATZ_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8404154.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="ACHATZ_001629"
     CDS             complement(4467..5036)
                     /locus_tag="ACHATZ_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8404155.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="ACHATZ_001630"
     CDS             5222..6124
                     /locus_tag="ACHATZ_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8404156.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="ACHATZ_001631"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="ACHATZ_001631"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8404157.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="ACHATZ_001632"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="ACHATZ_001632"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8404158.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="ACHATZ_001633"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="ACHATZ_001633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8404159.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="ACHATZ_001634"
     CDS             12692..12877
                     /locus_tag="ACHATZ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8404160.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="ACHATZ_001635"
     CDS             complement(12939..14135)
                     /locus_tag="ACHATZ_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8404161.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="ACHATZ_001636"
     CDS             complement(14305..15741)
                     /locus_tag="ACHATZ_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8404162.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="ACHATZ_001637"
     CDS             16034..16843
                     /locus_tag="ACHATZ_001637"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8404163.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4954 a         3539 c         2950 g         5438 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgacaa t
//
LOCUS       DAYYAJ010000061        16779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000061 DAYYAJ010000000
VERSION     DAYYAJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.061"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..313)
                     /locus_tag="ACHATZ_001638"
     CDS             complement(2..313)
                     /locus_tag="ACHATZ_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8404164.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(315..512)
                     /locus_tag="ACHATZ_001639"
     CDS             complement(315..512)
                     /locus_tag="ACHATZ_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8404165.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(906..2633)
                     /locus_tag="ACHATZ_001640"
     CDS             complement(906..2633)
                     /locus_tag="ACHATZ_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8404166.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2626..3819)
                     /locus_tag="ACHATZ_001641"
     CDS             complement(2626..3819)
                     /locus_tag="ACHATZ_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8404167.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4006..4650)
                     /locus_tag="ACHATZ_001642"
     CDS             complement(4006..4650)
                     /locus_tag="ACHATZ_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8404168.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4744..4878)
                     /locus_tag="ACHATZ_001643"
     CDS             complement(4744..4878)
                     /locus_tag="ACHATZ_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8404169.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4880..7012)
                     /gene="feoB"
                     /locus_tag="ACHATZ_001644"
     CDS             complement(4880..7012)
                     /gene="feoB"
                     /locus_tag="ACHATZ_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8404170.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7009..7482)
                     /locus_tag="ACHATZ_001645"
     CDS             complement(7009..7482)
                     /locus_tag="ACHATZ_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8404171.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7883..8107
                     /gene="nrdH"
                     /locus_tag="ACHATZ_001646"
     CDS             7883..8107
                     /gene="nrdH"
                     /locus_tag="ACHATZ_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8404172.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8266..10425
                     /gene="nrdE"
                     /locus_tag="ACHATZ_001647"
     CDS             8266..10425
                     /gene="nrdE"
                     /locus_tag="ACHATZ_001647"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8404173.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10475..11440
                     /gene="nrdF"
                     /locus_tag="ACHATZ_001648"
     CDS             10475..11440
                     /gene="nrdF"
                     /locus_tag="ACHATZ_001648"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8404174.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11529..12668)
                     /locus_tag="ACHATZ_001649"
     CDS             complement(11529..12668)
                     /locus_tag="ACHATZ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8404175.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12977..13690)
                     /locus_tag="ACHATZ_001650"
     CDS             complement(12977..13690)
                     /locus_tag="ACHATZ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8404176.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13944..14645)
                     /gene="nagB"
                     /locus_tag="ACHATZ_001651"
     CDS             complement(13944..14645)
                     /gene="nagB"
                     /locus_tag="ACHATZ_001651"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8404177.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14879..16411
                     /locus_tag="ACHATZ_001652"
     CDS             14879..16411
                     /locus_tag="ACHATZ_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8404178.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16602..>16779)
                     /locus_tag="ACHATZ_001653"
     CDS             complement(16602..>16779)
                     /locus_tag="ACHATZ_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8404179.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5211 a         3452 c         2888 g         5228 t
ORIGIN      
        1 attaaaattg agatttatct ccataatctg ctcgatataa accatttatt ccagcagttc
       61 ttccaatttc actcaaattt cttattaatt cctcttgata ttcagtgaat cttattttat
      121 tagtataagc agtaaaatat agataattca ccaatctatt tattaaagga ctgggatcta
      181 attcatattt ttgtttttct aattttaaat ataccttttg taaaacctca agaatatcag
      241 aatatttagg atctttactt atagatatat cattatataa ttcatgaaca atttgcttag
      301 catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc acctatagac
      361 attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt tttagtgcaa
      421 tacactccat ttccataata ttttccacta tgagtggtac ccccatagat aagttgtgtc
      481 tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa ttaatgtttt
      541 tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg aaacgtagca
      601 aaataattcc tgaataagaa tttaatcact tttaagtata taaactatgt atattcttta
      661 atattttttg agaatgcact tcgtacataa taaactttaa tcacattagt ctttcatacc
      721 tacgatcgat ctctttgact cgcacccata aattcataat agtttgtcgg cgtctttgaa
      781 cgtttcgaag atgagctacg cattctcttt ttttatagac aaaaaagata gagacgaaca
      841 tggagcttcc aaatttggat atttgatcca attttggaag ctccatttat ttcgtgtctg
      901 tctttttatt ttttccagct ttcgactaat tcttggttct ctttgatcca gtcttttgct
      961 gcttgctgtg gatcttttcc attgttgatt tccaacataa ctttttccat atctttttct
     1021 gaccaatgga aattgtctaa tactttgtaa gcttctggct ggtcttcttt caatcctttt
     1081 cgaaccatcg tatggatctg ctcttcgctt cccattccgt tttcaggatc ttctaagtat
     1141 ttcagatcat atttggcaaa catccaatga ggtgtccatc ctgtaactac aattggttcg
     1201 tgttttttga tcgcttgacc tagtgcaacg gtcattgctc ctgaagaaga tgtctctact
     1261 ttccagtctt tcaagttgtc atatttttgg atcgtgtttt cagcagccgt aactacacca
     1321 gctcctggct cgattcctat gattttctta cctgcctgat cggttaactc atcgatcgaa
     1381 tttacatcca tgtaggctgg cactgctaaa ccaacttttg ctcctgtcaa attggcacct
     1441 aggtcttcga cttgatcttt atattgtgca tattgtgaac catgagtttt aggtagccaa
     1501 gctgaaacca tggcatctga ctcgccattt gaaacagatt tccacatgat tgaattatct
     1561 aatggcgtca tcgttacatc atagcccatt tgtttcaata cttcaccgac tacatttgtt
     1621 gaagcaactt ctgtatccca ttcaacataa gataagttga tacgttttgt ttctttggca
     1681 gatgaaaaac tccctatacc aatcgcacct aagatcacta ctactacagc accaatgatc
     1741 agtccttgac gttttttact tttctgtgtt gttgctcctg cagctttttt attgtttggc
     1801 tggttcaaat gctgtgtaaa acggtcaatg atgattgcta agataactaa tgcaaccccg
     1861 ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag
     1921 ccgggtgcac cgatcataga ggcaataacg accattgaaa gtgacagcat tgttgtttga
     1981 ttgatccctg ccatgatcgt acttttagct aaaggcaatt ctagtttgaa tagtttttga
     2041 cgacttgtgc tcccgaatga atcagaagct tctactaatt ctttaggaac ttgtcgaatc
     2101 cctaaattcg taaaacgaac ggttggtggc agagcaaaga tgactgaggc aaatacccct
     2161 ggaaccatcc cgataccgaa aaatgcaact gctggaatca aataaacgaa tcctggcatt
     2221 gtctgcataa agtcaaggat cggtttgata atactttggg ctttactgct ttttgccatc
     2281 aagatcccta atggtacccc aatgatgatc gagatcacac tagataaaag aacaagtgtg
     2341 acagtattca tcaaatcatt ccatagacct tggttataaa taaaccatag accgattaat
     2401 gtgaacaacg taagtcctgg acgtttctta gaaataaaga aagctgctac cgtcaataac
     2461 aagataaaca aaggagctgg aatcactaac agtaaatttg taatccccga catcactgcc
     2521 tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg
     2581 atattttcta cccagtttgc cactggtaat tgatagttca atagattatt catgttcact
     2641 cacctcgctt gtttctgcta atgcttcaat cacactacct cggatgacga caccttttaa
     2701 ttttccatcc tctacaactg caagtggagt cggtgagtca taaataatat tgaaaatatc
     2761 acttaccacc atgtctttcc caatcaccgt cacattttca tctaggaagt cgatcaatgg
     2821 acgtttttgc ttcctagctt ctaacgcttg atctgcagtg atgatccctt tcaggtgtct
     2881 ctttctgtca acagccatca gcatgctgac ttcttcatta cgcatccttg tcaatgcaac
     2941 gtttggtcca tcagattcga tattcgtcgt caatgcaggt accatgatgt tttgagccgt
     3001 aagcactttg gaacgatcga cttcttccac aaattcacgt acataatcat ttgctggatt
     3061 ggtcaatatt tcttcacctg ttccgatctg catgatttcg ccgtctttca tcaaggcgat
     3121 tctgtcccca atccgtaaag cttcattcaa atcatgggta atgaaaataa tcgttttttg
     3181 aacattggct tgcaggtcca gtaattcgtc ttgcatttct cgccggatca gcggatcaag
     3241 agccgagaac gcttcgtcca tcagcaagat ctcaggatca tttgctaaag ctctggctag
     3301 gccaacccgt tgttgcatcc caccagataa ctgactagga tattggtctt taaatgaaag
     3361 caaactagaa ttttctaatg ctttttctgc tttttcttgt cgttcttctt taggtacacc
     3421 acggacttct aacccgtatt ctgtattttc caagatcgtt cgatgtggaa acagaccgaa
     3481 gttttggaaa accatattga ttttatgacg acggacttct cgcaattctt ctttgcttaa
     3541 cttcgataca tcttctccat caatatagat actgcccgaa gttggctcaa tcagacgatt
     3601 caatagacgg atcaatgtgg atttcccact cccggaaagc cccatgatca cgaagatttc
     3661 tccttctttt acgtcaaaat tcacgtcata cacaccaacc gttgcaccgg ttttctttaa
     3721 aatctccgtt ttcggctggt gatcattcat catatctaaa gcggcttgtg tctttttacc
     3781 aaatatcttt gttaaatgtt ttacttctac ttttaccaaa caaattctcc ttttttatgt
     3841 aaattcattt ttctttctga aaaaagtgac cacactatgt taacatcatg tagtcacttt
     3901 gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa acttggctaa atctttgtaa
     3961 ataaaaggtt tagccaagtt aatatttttt taatatttca cacaatcatt ctgcaagctg
     4021 atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta tattcatttc caataaaatg
     4081 aacgatgtcc cctgccgtaa atttagcata ggggccaggt gaaacaagca gttcttcatt
     4141 atgagaaatt gcgataactg tggcgccagt cgcctgccag atattcaact cgctaattga
     4201 acggtccaga tgcattgctt tttctgataa agataattca aacggaagta aaggattcat
     4261 cttatgtatt cgcttcgttt gattgaccag ctgatccaat aattgagaaa aatgattcag
     4321 ctcatcttgt tgcttctcca cacttgccat gatttctgct tttgtatctt ggatggattg
     4381 tacatcttga tattgcaata ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc
     4441 acttccatgc cgtacttcca tgattccaag atctaccagc acattgatag ccttgcgggt
     4501 agtctcagga gaagcattgt acgtactggc aagtgttgag cgggcatgga tcttctcacc
     4561 cactttatat cggttttcaa cgatacgttc ggctaaatca acagcaatct gctggtagcg
     4621 cgggcggacg atttttttct ttccagacat tttctttcct ctttcttttt ttactttccc
     4681 tcatcttact atgctctagc aaaataagca aaaaagggga gaatagcctc ttgcaacgac
     4741 tgatcatcgc ttgatctgtt ccttttttac tgggcaagct gtttgacagt catcacaatt
     4801 cttaccggat tttaaacgag tatagacaat tgcgcctgca cttccaaaga ttaacacact
     4861 taataagaat gtcgccatcc tattcctctc cttctaatga caatatagta taaaactggt
     4921 tcttggattt tggctgacgg aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa
     4981 gatacgtccc taacgctacc tgtccaccct ccaataagtg tcccaactga taaatcacaa
     5041 aactaactaa ataagctaaa ccgcattgat agcctatggc gatccaagtc catttcttgg
     5101 cattcatttc ccgatggata gccccaattg ccgcaaaaca aggcgcacaa agtaaattga
     5161 aggctaacaa tgaataagct gctgcgggag tatatgtgtg ttgtagcact tgccagattt
     5221 ctctgccatt ttcggatact tccgcatgac caaacaaaat accaaatgtg ccaataatat
     5281 tttctttagc gaccaatcct gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa
     5341 gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc taagatactt tggtcttcag
     5401 aaacaggttg agcataaaaa ttataagaag agctgaacca gatcacgata ctagaaacaa
     5461 agataatcgt tcctgctttt ttaacaaaag acttgctgcg gtcataggtt tgtcttagga
     5521 ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat gataaaagga gctgtatctc
     5581 cagcaaacaa acgtgttttt ttcaagctga ttccagagaa aataattgca gtcataccaa
     5641 gaaaataggc agaaggagct acccaagatt gatgaggaaa aaaggcccct gctactaagg
     5701 caataatcgg caactttgca gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac
     5761 gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc tggcactcca catcctgtag
     5821 caatcagcat aggaatgaag gattttcctg aaagaccgaa tttcctgaaa agtcgatcca
     5881 tgacaaaagc gatcctcgcc atatatccgc agtcttccag aaagctcaag cataaaaaca
     5941 atataatcaa ttgcggtaga aatcctaaca ctgctcccac gcctgcgatg atgccgttca
     6001 agatcagctc ctgcatccat cccgctactt gccattcagc taacaatctg ccaacatgtt
     6061 ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc tgtccccatt gttcctactg
     6121 tttgaatagc cagataatag atcagccaca tcacaaatgc gaagatcggc aaggctaacc
     6181 aacgattcgt tgcaatctga tcgattttgt cactcatcga taattggaaa gaatcatttg
     6241 acacaacaca gagtgtacat agttgggtaa taaattcata acgttcattg accagaatcg
     6301 tctcactatc gtcgtccaat agtttctctg ttaactggat cagttgctcg atctcttttt
     6361 gctgagaaac tgctatctcg taatcctctc ttaccttctg atctcgttca aaatatttca
     6421 ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag
     6481 ctgcttctaa acgtggatca tatgtacagg gaacaggaat agaaggccgc ttttttgcag
     6541 actcctgaat cttttcgatt agtttttcga ttccttgctt tttcaatacg ctgattggta
     6601 aaactggtac accaagacca taggaaagct tttctagatt gatttttttt ccttcttttt
     6661 tgatgacatc catcatattc agaccaatga tcataggaat gtctaaatcc aataactgcg
     6721 tagttaaata aagatttcgt tctaaattgg tagcatcaat aatgttgatg atcagttcag
     6781 gctgttcctg aaccaaataa tctcttgcaa tcacttcttc tggtgtataa ggagacaaag
     6841 aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac gatccttctt
     6901 ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca gtcaagagat
     6961 tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc attctttttc
     7021 ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt cataccctcg
     7081 cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta tttttacatt
     7141 tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac
     7201 gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt cagaggaagc
     7261 agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca ctcgatgact
     7321 ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt tacttccggc
     7381 aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga ttcgttcgac
     7441 ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc ctctcttgtg
     7501 aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg tgttacgcaa
     7561 ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc aattatactt
     7621 gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa atatttcttc
     7681 atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga tacttgtttt
     7741 cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac acaatatgta
     7801 gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag ataataagaa
     7861 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
     7921 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
     7981 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
     8041 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
     8101 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
     8161 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
     8221 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
     8281 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
     8341 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
     8401 aatacgatga cttttccatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
     8461 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
     8521 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
     8581 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
     8641 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
     8701 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
     8761 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
     8821 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     8881 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     8941 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     9001 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     9061 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     9121 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     9181 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     9241 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
     9301 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
     9361 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
     9421 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaaca
     9481 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
     9541 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
     9601 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
     9661 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
     9721 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
     9781 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
     9841 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
     9901 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
     9961 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
    10021 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
    10081 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
    10141 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaacggca
    10201 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
    10261 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
    10321 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
    10381 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
    10441 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
    10501 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
    10561 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
    10621 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
    10681 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
    10741 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
    10801 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
    10861 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
    10921 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
    10981 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
    11041 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
    11101 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
    11161 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
    11221 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
    11281 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
    11341 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
    11401 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
    11461 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
    11521 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
    11581 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
    11641 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
    11701 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
    11761 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
    11821 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
    11881 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
    11941 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
    12001 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
    12061 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
    12121 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
    12181 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
    12241 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
    12301 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
    12361 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
    12421 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
    12481 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaatt
    12541 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
    12601 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
    12661 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
    12721 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
    12781 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
    12841 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
    12901 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
    12961 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
    13021 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
    13081 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
    13141 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
    13201 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
    13261 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
    13321 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
    13381 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
    13441 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
    13501 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
    13561 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
    13621 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
    13681 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
    13741 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
    13801 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
    13861 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
    13921 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
    13981 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
    14041 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
    14101 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
    14161 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
    14221 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
    14281 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
    14341 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
    14401 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
    14461 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
    14521 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
    14581 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
    14641 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
    14701 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
    14761 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
    14821 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
    14881 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
    14941 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
    15001 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca
    15061 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
    15121 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
    15181 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
    15241 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
    15301 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
    15361 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
    15421 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
    15481 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
    15541 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
    15601 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
    15661 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
    15721 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
    15781 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
    15841 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
    15901 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
    15961 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
    16021 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
    16081 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
    16141 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
    16201 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
    16261 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
    16321 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
    16381 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
    16441 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
    16501 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
    16561 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
    16621 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
    16681 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
    16741 ccagccgcac ctccaataaa gtacataaac ataaataca
//
LOCUS       DAYYAJ010000062        16717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000062 DAYYAJ010000000
VERSION     DAYYAJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.062"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..1465)
                     /locus_tag="ACHATZ_001654"
     CDS             complement(254..1465)
                     /locus_tag="ACHATZ_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8404180.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1584..2219)
                     /locus_tag="ACHATZ_001655"
     CDS             complement(1584..2219)
                     /locus_tag="ACHATZ_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8404181.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2355..3008)
                     /locus_tag="ACHATZ_001656"
     CDS             complement(2355..3008)
                     /locus_tag="ACHATZ_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404182.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3111..3317)
                     /locus_tag="ACHATZ_001657"
     CDS             complement(3111..3317)
                     /locus_tag="ACHATZ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM8404183.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3393..4139)
                     /locus_tag="ACHATZ_001658"
     CDS             complement(3393..4139)
                     /locus_tag="ACHATZ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM8404184.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4299..4523
                     /locus_tag="ACHATZ_001659"
     CDS             4299..4523
                     /locus_tag="ACHATZ_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM8404185.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4547..5308
                     /locus_tag="ACHATZ_001660"
     CDS             4547..5308
                     /locus_tag="ACHATZ_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8404186.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5320..5577
                     /locus_tag="ACHATZ_001661"
     CDS             5320..5577
                     /locus_tag="ACHATZ_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404187.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5669..5920
                     /locus_tag="ACHATZ_001662"
     CDS             5669..5920
                     /locus_tag="ACHATZ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8404188.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5896..6069
                     /locus_tag="ACHATZ_001663"
     CDS             5896..6069
                     /locus_tag="ACHATZ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404189.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6183..6590)
                     /locus_tag="ACHATZ_001664"
     CDS             complement(6183..6590)
                     /locus_tag="ACHATZ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404190.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6751..6975
                     /locus_tag="ACHATZ_001665"
     CDS             6751..6975
                     /locus_tag="ACHATZ_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404191.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6985..7179
                     /locus_tag="ACHATZ_001666"
     CDS             6985..7179
                     /locus_tag="ACHATZ_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404192.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7182..8204
                     /locus_tag="ACHATZ_001667"
     CDS             7182..8204
                     /locus_tag="ACHATZ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8404193.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8167..8982
                     /locus_tag="ACHATZ_001668"
     CDS             8167..8982
                     /locus_tag="ACHATZ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM8404194.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            9000..9782
                     /locus_tag="ACHATZ_001669"
     CDS             9000..9782
                     /locus_tag="ACHATZ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404195.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9782..10630
                     /locus_tag="ACHATZ_001670"
     CDS             9782..10630
                     /locus_tag="ACHATZ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8404196.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10627..10869
                     /locus_tag="ACHATZ_001671"
     CDS             10627..10869
                     /locus_tag="ACHATZ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404197.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11012..11380
                     /locus_tag="ACHATZ_001672"
     CDS             11012..11380
                     /locus_tag="ACHATZ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8404198.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11395..11556
                     /locus_tag="ACHATZ_001673"
     CDS             11395..11556
                     /locus_tag="ACHATZ_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8404199.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11553..11879
                     /locus_tag="ACHATZ_001674"
     CDS             11553..11879
                     /locus_tag="ACHATZ_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404200.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11876..12691
                     /locus_tag="ACHATZ_001675"
     CDS             11876..12691
                     /locus_tag="ACHATZ_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8404201.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12693..12818
                     /locus_tag="ACHATZ_001676"
     CDS             12693..12818
                     /locus_tag="ACHATZ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404202.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12830..13141
                     /locus_tag="ACHATZ_001677"
     CDS             12830..13141
                     /locus_tag="ACHATZ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8404203.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13138..13383
                     /locus_tag="ACHATZ_001678"
     CDS             13138..13383
                     /locus_tag="ACHATZ_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404204.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13380..13796
                     /locus_tag="ACHATZ_001679"
     CDS             13380..13796
                     /locus_tag="ACHATZ_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8404205.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13793..14131
                     /locus_tag="ACHATZ_001680"
     CDS             13793..14131
                     /locus_tag="ACHATZ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404206.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14128..14358
                     /locus_tag="ACHATZ_001681"
     CDS             14128..14358
                     /locus_tag="ACHATZ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404207.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14365..14568
                     /locus_tag="ACHATZ_001682"
     CDS             14365..14568
                     /locus_tag="ACHATZ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8404208.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14565..14861
                     /locus_tag="ACHATZ_001683"
     CDS             14565..14861
                     /locus_tag="ACHATZ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404209.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14938..15351
                     /locus_tag="ACHATZ_001684"
     CDS             14938..15351
                     /locus_tag="ACHATZ_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8404210.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15364..15750
                     /locus_tag="ACHATZ_001685"
     CDS             15364..15750
                     /locus_tag="ACHATZ_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404211.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16301..16528
                     /locus_tag="ACHATZ_001686"
     CDS             16301..16528
                     /locus_tag="ACHATZ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404212.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16525..>16717
                     /locus_tag="ACHATZ_001687"
     CDS             16525..>16717
                     /locus_tag="ACHATZ_001687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404213.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5667 a         2726 c         3485 g         4839 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gttcgttatt taatggtatt ttaaatgaag ataagcgaat ttacagcatt tttaaatgct
      181 tatataccgc aaattatccc aagtttttgt atatcctacc cgagtttata cccgaaaata
      241 taatttttta gtgttatttt atatcttttt ttaataggtt ttcaaacgct gtagttgctg
      301 tatctttttc tttgcgtaag acatggccat aaacattcat tgtagtatta aaatctcggt
      361 gtcccaatcg tttttgaagc tcttttggag tcatattagg atcgctaagc aaataggtag
      421 ctgatgtatg tctaatgtcg tgaaaacgaa tgtgacgcaa attgtgacgt ttggtgaatc
      481 gtttgaactt cttatacaga taacttccac gtggcaaagt tccatcttgt tttgtatcaa
      541 atataaaata gtgttttaat ttctttatat ttaatttcca acgaatcttt ttattctctt
      601 tgattaattc atctaacata tcaaatagat aagcaggacc ggttactatt ttttcatcat
      661 catcattttt caaacctggc agcaaccgaa tatctgattt tccatccact tctgaaatcc
      721 gctgatgaaa tcttatttct tgtttgtcaa agtcaatatc tttttcttct aaagctgcaa
      781 tttctccttg tcttgctcct gtaataaatg ctagaacaat taaagtttta atttctatgc
      841 tttcctcaaa tgctgctttt agcattgcat gaatttcttc ttccgaataa ggttcttcga
      901 tattcttttt tacgcctttt tcttttggta tttttacatt ttccacagga ttttctttaa
      961 ttgctccgta ctcattttta gccatcaaaa ataagttgtt aacggcgtat aacatacgaa
     1021 gctttgtatt tcttgataaa ggtttatcag agtttctccc aggatctttt gtattgattc
     1081 tttgagcatt aacaacaatt ttctttatca tgtatggttt gatatcgatt agagggataa
     1141 aatcaaattt atcaagaaat ctattctcga tcattcgctt atagttatga taagactttg
     1201 gctctaaatt tttttcggcc tctgaaagcc atatttttct ataaaattca ccaaaagtta
     1261 tattgaacaa atccaacgaa tcatcggtca ttccttcgaa ttgctctaac cagatgttca
     1321 attctttata tgccgctctt ttactacttg cttttatagt tttgcttttt ctttttgccg
     1381 agccatcagg tttgtatcct aatacagctc ttagtctata tgtttcgttg tctacttgtt
     1441 ctaagtatcc tgttacttct gacataattg aatcagctct ctttctctga tacaataggt
     1501 actataaaga agcctatcgt ataggtttgt ttctttctta gaacacgctc gctttggtcg
     1561 gtggggcgtg ttttttatac taattagttg tgaagagcaa ttgaaactgt ctctgttcca
     1621 aagtaacctg tttgtgcttg caatacatta ttttttgcat ttgcttgggc ttcagagata
     1681 tcaaaaacta ttttaccagt ttgttgcata tcaggattta agctttccat aaagaaagag
     1741 ttagtgatat taccattttc atcttgattt gccgacatag atgcagccga atcagcttcg
     1801 aaagtttttc catctgcttt taatttgaag aatgagctat caacagtcac agccttatca
     1861 cctgcatttt ttacagataa gtcaactact aagaaagtgt cttttgcgtt agtaggaagt
     1921 acagatgggc caacttgttt tactacttca acggagttta ctttgtattc cattttccct
     1981 acagcaacgt catcaccgat tttataagtc ttttcttctt ttgttgtagt ttcttttgta
     2041 gtttctttat tagtacttgt tgatgttgca gtagctgaat ctttgttttc accgccactt
     2101 agagcaccac caataccaaa aaacaaaagt actattaata cccaaaacca tacccgttta
     2161 taaaacggtt ttttaacctt atacattttc ccatcttgac ccataacttt ttttgccatt
     2221 taaatattcc tcatttcttg ttataatata tttgtgatct cagaaatgag gtatgagtcc
     2281 gtgttgcagc acgggctttt ttatttataa cttttttaga gattataggc aaaatagtag
     2341 ggcataaaaa tatattattg aattccgtat ttaaaaaatc ctaattgaac ttcaccggaa
     2401 gtcttttgct gtgttgtacg caatgcttct tcttcagaca ttccattctg tactttccat
     2461 gcaacaggcg acatcccgta tttgttaaca aaatcagtaa gtgataaagt gtcagcgtct
     2521 tgctgagcgc ttgtttgttg gtcttctgga ttttgttgag atgctgcttg ttgttctttc
     2581 tgatcttgac tgataatatt gccagcatca tctgtagtca atccattttc ataaagggcc
     2641 acgccgaaag cttcccactc tttgttggac caatttgcac gatcagctgg agttgactgt
     2701 aaagtgcgtt gtttcatctg ttcatatgtt tcttcttgag gtgcggtttg gattgtactc
     2761 tgactggagc ttataactgt tgggctaggt tccgccgtaa cttggttgga gcttgtaact
     2821 gttgaactag aatctgtttt agatgtagat ttgctagtag atgaactggt ttcagttgtt
     2881 tcttttgttt tacttatttt tgtttcttgg ttagaagtgg catctgttga ttcagctttt
     2941 ttattatttg aacaagctga aagtagcaga gcagtactta acaacaacat aacgctaact
     3001 tttttcattt tatactcctc ttttctttga tacaataagt tgtaaagaag cctactatag
     3061 gtttaacatc ccacgtccac aaactttggt cggtgggcgt gtttttttgt ttatagactt
     3121 tcgaaaacta aagtagcttg gattctatcg ccaccaccga aacctttact accaccgttt
     3181 gtagtggaaa tagtgtgtaa tcgataacct ttagaacact gtctatttat agtgttttca
     3241 agttctgata ggttttgcga cccttttcct ataaactttt cttttaatac aacttgcaat
     3301 acaacgtaac ttggcatatt cttttccttc tttctactat gataataaat actaacccct
     3361 agccggagcg ccaactacga ctggggtatt ttttagaata cgactttccc gattattcta
     3421 atctgttccg gtgagacgac tctatcttca tataattcgt ttatagagcg tagaataatc
     3481 gtttgactct cataatcctt atatacttct ttacacgtca caccgtcacc atctatttca
     3541 acgatagcaa tctcaccgtc ttccacgtct ggctgttcct taacaaatac aaattgtcca
     3601 ttcctgataa ctggttccat tgaatcgcct ataataggaa cagcaaaatc agcattgttt
     3661 ggaactcttt caaattcttc ttcctctaca actgtatcgc cataaactaa ttcagtcggg
     3721 ttggcggcag aagctgaatt tttgattgtc ggaagttttt cctttttagg aaagtgcacc
     3781 acgtttcctc gattttgctc ttcgagttgc tgttcagcaa agttgtagac ttttgtttgt
     3841 cttggttttt ctaattgatt gtagattgtt tcaatggatg gaatgttatt tttcttatct
     3901 tctttaggaa catcgtaacc aagcaaccat gcctcactca catttaaagt tttagccaac
     3961 aaataaagct tgtgttggtc gggcgatgac ttaccattga catattgcga tagatgactt
     4021 tttgacattt ttattccagt ttctttttgc aaaggtaaag acatattcaa aatatcaact
     4081 tgctttaaat ttctttcaga cattaactga ttaagtcgtt gctgagtcgt ttgcttcatc
     4141 ttatacctcc tttctgatac ttattatata ccttattgaa cataagttca ataaaaaagt
     4201 taaattaatt tgaactttta tgttgacaat gttttatgca ggagttatac tttggttaca
     4261 aagttcaaaa catttgaact agaaaggagc gaactaatat gagttttgat tattctaagt
     4321 tgtcgggaag aattgttgag aaatatggaa cgcaatacaa tttttcaatc gctttaggtt
     4381 tgtcggaaag aagtttgtca ttaaagttga ataaccgagt accatggaaa agcaccgaac
     4441 ttcaaaaagc cattgaattg ctagatattc caaaagaaga aattggcgaa tattttttta
     4501 atttagaagt tcaaaatgtc tgaacttaag aaaggaattc gaaaaaatga acacacaaaa
     4561 atttgaaaac aatctatttg aattagaagt taagacggaa aatggtgaat cgttatttaa
     4621 tgctgaaaca gtagcaagaa gtttgggttt cgtggaaaca aaaaacggta aagaatatgt
     4681 tcggtggaga actgtcaatg gatatttaaa aaaatacctt tcgcaagaag ttgcgaaaaa
     4741 cgactttatc agtgagccaa tggtttacaa attagccttt aaagcaaaca acgtacttgc
     4801 tgaaaaattc caagactggt tagcaacgga agtcctacct caaatccgaa aacacggcat
     4861 gtacgcaaca gatgaactac ttaacaatcc agacttgctg attgaagtag caaccaaact
     4921 caaagaagaa cgcacgttac gattgatagc tgaacaacga gtaaatgaat tacaaccgaa
     4981 ggcagactat tacgaccgaa tcttgaacaa caaaggatta gttacagtta gtacgattgc
     5041 taagaactac ggtatgagcg ctgtatcgtt taataaattg ctacatgaat taggtatcca
     5101 gttcaatcaa agtggcacat ggctgctata cagtaagttc caagacaaag gatacacgca
     5161 tattgagcca tttgattatg aagataaaaa cggcaatcgg caagtaaaaa caaggatgaa
     5221 atggacgcaa aaaggacaca tcttcttata tgaaacattg aagaagaata attacttacc
     5281 gatgattgaa cgtgaacaaa cagcatagga ggtaacacta tgaacgagga aatgagccaa
     5341 acaatcaagg tacaaaaaat gatagatgat ctaacacatg caattgatag ccaagccgat
     5401 aaaatcatca aggaattgca aggacaaaaa gtttgggatg caaaaatgct tttcaagact
     5461 atcaaatttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga agaaaagttg
     5521 gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa cagctgatgt
     5581 tttatagatg acagtttcat ccgaatctat atcattatag attcgattat tgcaatgtac
     5641 gaattataaa tacaggagga aataaccaat ggagcaacta gctttagtta acctctcaga
     5701 ccttaaagct tctttggcag aatctgagat tgcaaatgaa gtgtgggata caaagcaagc
     5761 tgcggaatat ttaaaaacca caacacgcac gctgactaaa gatgccgaat ctggaaagat
     5821 tccagcagcg aaagttggca gagagtggag gtttagtagt atcgctttgt atcagtatct
     5881 aaaaggagga aaaacatggg taaattcaac agagcactag tgttcagcgc acccctaatc
     5941 gtctatgctt taggactttg gggaagcaga caagcattaa tcggcacgat cgtttacatg
     6001 gtttggattt ttatggggct tgatgaagct gagtacagag cgaaaaagcc caccgagggg
     6061 gctgactaaa gtgtggttct gtttattagg tgtttatctc gtggccgttt tagtaaataa
     6121 ctacatgaga aaacgtggcg aatactggta tacatcctat gcggttctag tatgtctaat
     6181 gcttacaatt tttctaatga tttattcaaa ataggtaata ctttgtcaat caaataagtt
     6241 tcaagagatt tttgttctgc atcagtgttg ccgtaaccaa aacggcttaa gaaaattttc
     6301 ataacctcta tatcttcatt ttgaatatat ggcaggactg cataacctga tgcttttaca
     6361 gcagatatat ttttatcagt gttactacca atacaaatac ccacattgtt gagaaatgtt
     6421 gagaaagttg tttgtattgt ttgggttttt gaatcgtgtt tttgtaattc aatttcttta
     6481 tccttcatcg attcaacatg cttattattt ataaccgctg taatcgaagg ggatataaga
     6541 gctactaaag ctaggataat cgaaattgtg atcgtgttat cgaaactcat tttttcacca
     6601 tccagttttt aaaccattat atcaaaaagg agagaagaaa taatgcaaga attagtaatt
     6661 ttgaaaaata aaaaaagaca cgaacgccag caagcatgat cgtgtccaaa agaaaaatct
     6721 tttaaaccat tataacacag gaggaaataa atgatctcag ttaaagggtt aggcgatgaa
     6781 atattcgaag caatgatgag caaagcacaa caggacatca aagaaaaaat catttctgct
     6841 gcagcgcaag gacaaactag ctgcactgtt cgatcgagag gaacgacacc aagttttctt
     6901 ttggcgctag aagatgaagg cattttgaat atcaaacgaa gagatggcag cgtcagattg
     6961 ttttggcaat tttaggaggc cgtgatgaaa gattttgatt ctttaggcgc cagacagcaa
     7021 ccaccaaatg aagctagtcc tgttggagtt gattggcaag aaaatccgtt atatccaggt
     7081 gatacttgct atctaacaga agaaggctat gtaccagtag acgcaattct agagtacgtc
     7141 cagcaacatt atccaaagat tgaattagga ggaatttagg aatggcaaat gatttaacac
     7201 aaacaacaca acgttcatta gatgagcagg taatcagtaa tcttggacgg ttacaagaac
     7261 aaggtttaga aatgccacca ggatatagtc cacaaaatgc gttaaaaagt gcttttttcg
     7321 aactcactaa caatacagga ggaaacttgt tgcagatggc tgcaaacaat caagaaatga
     7381 aaacctccat ttctaatgca cttttagaca tggttattca agggttatct ccagcaaaaa
     7441 aacaatgcta tttcattaaa tacggaaaca aggttcagct aatgcgttca tattttggaa
     7501 caatggcggt ccttgatcgt gtaactggag gggcagatat tacgcctgta gtcgttcgac
     7561 aaggtgatga gtttgaagtt gcaatggatg gaccaaacat ggttgtcaaa aagcatgaaa
     7621 caaaattcga gaatttagac aatgaaatca ttgcagccta tgtagtcatc aagttaacaa
     7681 atggtaaaga gaccacaaca gtcatgacga aaaagcaaat cgatcaaagt tgggctaagt
     7741 caaaaatgaa agattctgga ccgcaaaagg aatttccaga agaaatggct aaacggacag
     7801 taatcaatcg ggcagctaaa acattgatca acacaagcaa cgacaacgat ttattagttc
     7861 aagctgcaaa agatacgtta gaaaatgaat ttgacaatga tcggaaagat ataacgccac
     7921 aaacagaaaa agtggctact ctcgaacaaa aattcttttc aaacaaaaag attactgaac
     7981 caatacaaaa agaaccagat ccgattgtga ttccagatga tatccaaaat gaagtaactc
     8041 gagttgctga tgtacctgcc catccagaaa tagagcaagc acatttgatc gaaaatgagg
     8101 atatcaatcc aattcaagaa gaattgatag atattccaga ctttggacgc gaggaaggtg
     8161 tagaagatgt ctcagaattt gaagacgacg agtacccttt ctgatgagaa ttattactcc
     8221 aatgaagctg actggcagta catgtcaaca tcacaatata agtccttttt aaaatgtgaa
     8281 gccgcagcct tagcaaagtt gaaaggcgat tgggcaccga cgtcggatcc caaagcctta
     8341 ctcgtaggaa actacgtaca ttcttatttt gaatcaaaag aagtacatga agcgttcaaa
     8401 gaagaaaata aatccaaaat gttttctagt aggaagccgt atggattgtt gaaagatttc
     8461 caaattgctg aacaaatgat tgaacgactt aagcaagaag aagcattcat gaacatttat
     8521 caaggtgaga aagaaacgat cgtgactggg gaactatttg gaacgacatg gaaaggcaag
     8581 attgattgct tgaatgttga ggatgagtat tttgttgata tcaaaacaac caaagacatg
     8641 catgagcgga aatggaacga aaactacgga tcaagagaaa cgttcattgt caattttggt
     8701 tacgtacttc aaatggcaat ctatcaagag cttcttttac agcaatacgg aaagatgttc
     8761 atgccaatca tcgctgcggt atcgaagcaa acgcctagtg aggcgaagtt gataacgatt
     8821 gatcaagata agatgagcta cgagctaatc atgctaaaag aaaatattga aagaattgtc
     8881 aaagtgaaaa atggcgaaga aaggccgata ccttgcggta cgtgcgagta ttgtcgaggt
     8941 aacctttcaa ttactggatt cactagcatg gacgatcttt aaaaggaggt gctttgcgaa
     9001 tgaatctagg atacatcaag ttataccgta aagtgactag ttcattcgtt tggaccaatt
     9061 ccgatatgtt caagctatgg atattgtgtt taatgaaggc tagtcatgaa gatagaaaat
     9121 ttttatttaa cggtcaagaa gtacgtttga caagcggaca attcgtcaca ggagcccatg
     9181 caatagcaaa agagtacaac gaaggagtcc cgagtgacaa agcaattgca tggcgaacgc
     9241 tatggagatg gcttaagaaa tttgaaaatg aagaattatt gacaatccaa tcgaacgcta
     9301 gatatagcgt tataacaata ataaattggt ctgactatca atcgagtgac aagccagtga
     9361 caagcgacgg acaatcgagt gacaagccag tgacaacaaa caagaatgat aagaatgata
     9421 agaatgataa gaataataat cctcgcaact ctcgaaaaac acgagagtat gcagatgacg
     9481 atccaaataa aaaattggcc attcttttat taaaactcat tcgaaagaat cgtagtctca
     9541 aagaacccga tttgaacaaa tgggccaata cgattcgttt gacgattgaa gctgacaaac
     9601 gaactggaaa agaagttcaa gacatgattg tttgggctac taaccatgat ttttggtcca
     9661 cagtcatttc atcgccaact agcttaagaa aaaatttcga tacaatggtg ggtcaaaaaa
     9721 ataaaagaaa gcaacaaaat atttctaatg atgagttacc agaaacaggt gaggattggt
     9781 aatgaataaa aaactaagtg caatggctgc accttacggc ggattaaaaa cagcagatca
     9841 taattgcccg aaatgtggtg atccattgta tatctggaaa acaaaaaata aagatggtac
     9901 tgatcgatgc ggtcctacat gtatcaataa gatttgtggt tatcgagaaa tggtaactaa
     9961 aaatcaaaaa gaagctatcc aaaaagcgaa tgaagcaatg aaaaaggacg ctatcaatcg
    10021 aatgcttaac agttcaatga ttacagatga tgccatatgg accttcgatt ttgatggata
    10081 caaagtagtt gatcaggaaa cagcacaaat aaaagcgatg gctcaagaat gggctaaaaa
    10141 aattgtaaat ggtagcacga ttcatgcagt gataaccggt agaacaggag ccggcaaaac
    10201 ccatctaggc gctgcagtga ttaaagaagt catgagggca tctaattata aaattgcttg
    10261 ttcatttatc agttatcgag aacttttaga acagttgaaa tttgcaatga atgatccaga
    10321 agcaagaaaa gccgtaacag gttcgctgat ggctgagata aagaaatcag atttcgtggt
    10381 aatcgacgat ttgggtgcag aactaggaag aatggaagaa aacaaccagg caacgcctta
    10441 tgatgttgat gttctcacat cgctcacaga agctcgtcta aacaaagcta cgatattcac
    10501 aaccaattta tcatcgaagc aattaaaaca cgcgtacggt gagcgagtgt tctctcgtgt
    10561 aatgaatgga acgaaaggga acatagctgt attcaaaaca acgacagaca aaaggaggaa
    10621 tccagtttga cctttgtagt gaaaaaaatg tgctacttag atagccgagg gcgaggagaa
    10681 gctagtgtcg agtttgctaa acaccacacg actaaagaag aggctgacct tgttgcaagt
    10741 gtttgtggcg gcgaagtagt cgaagttatt aaacccaaaa gacgattttc aaaacctaag
    10801 acaaagccag tgaaaaaaga gtgttgttgt ccgaaaagta accaagcatg gatgagaggt
    10861 gcaaaatgac ttgtttaaaa tgcaacgatg aaacagttat ttggtataag acattgcttg
    10921 gatggtcaac ttgtgaacct tgtccaatat gcaatgaaaa tggacgacgt tcgaaagaac
    10981 gactcgaaag actaaagaag gagtatagca aatggcaaca agaagcaaat acggaaacaa
    11041 aaagcacgaa gtagacggca tcacttttga ttctatcacg gaagcagagt attacgatct
    11101 agccttgtgg caagcacagg cgaatggttg gaaagtgaag ctccaggaaa ggtttgagct
    11161 gatgccgaag tttgaactag agggcaagaa gtatcgcaag atcgagtata ttcccgactt
    11221 cacattttac aaaagcggca agctggtcaa agtcgtagat gttaagggga tgcagacaaa
    11281 agactttaag atcaaggcaa agttgttctg tcatcaatat caagtgccgt tgatattagc
    11341 caaaaaatat cggaatacgt tcaaggaaga gcgtttttaa cgaggtggtc catcatgaca
    11401 acagaagaag tgattcaaat gcgaattcga agccttcggc gtgagattga cgatctggaa
    11461 cgaacaaagg cagtgatggt caatgaaacg gctagaaagg caatcgattt gcacatagag
    11521 aatttaagaa gggaaatccg tagattggag gaatgagcgt ggataaggaa gcggcttggc
    11581 gaaaattaat gttgctgatt caagatgaga actggcaaga agatgaagca gtggttgctg
    11641 aagttcagcg tctaggaaag attgctaacg gacgtatacg aaaaaagcca gacaaaagaa
    11701 aacagcgcaa agggaaaatc gtcgttgttt tacacgaagg caaaattttg atgcaaggaa
    11761 cagctagtga gctgtctgca gaaactggat atacgcgtgg gactattcga acgtacgctt
    11821 ggcgaaatca caccgatcga aaagggcacg aatataagta tttggaggaa gaaaaatgaa
    11881 cgaaaacaaa ttaatcaaat tgggtgtagc aggagcagta atcgtaggta ttggagttat
    11941 cggaggattt aagttcttcg aaaaaatcga taatggatat gtgggtgtgc gctattcaat
    12001 gaacggcggt atcaaagatg aagcactgac gcaaggtgtg aaatttgtag ggattgacaa
    12061 agtgatccaa tatccaattc gcttgcaaac tatccaatca aaaaatattt cagtatctac
    12121 aagcgacggc aaaaagacaa cgattgatat caaatatgac tacaaagttg attcaactaa
    12181 agcagcaaaa atgtacaagg aatttgggaa tatcacttcg gaagatatcg aaagtggatg
    12241 gttaaaatct aagcttcaaa aggtcgctcg tgaagtttat gcgaaatata gtctgcttga
    12301 tgtcctttca ggagattcct ctaaagttga agctgaggta ttaacgaact ttgctaaatc
    12361 agttgaatct aaagggtttg aagtcgaaga cgtaacactt ggtgttccag atgtcgataa
    12421 agaaacacaa aaatcaatcg atgcgatcat tcgagctggt caagaaaatg aaaaagcgaa
    12481 gctagacgca gaaactgcaa aaactcaagc tgatagtgaa gcttacaaga aaacaaaagc
    12541 tgcagaagca gaggcagaat ctaatcgcaa agtcgccgaa tcagtaacag acaatttgat
    12601 tcgttatgaa gaagctcaag ctcgcaaaaa gcatggatgg gtaacagtaa atggagcaga
    12661 tactgtagtt acggatgaag caggcaaata atatgggatt ctttatggct aaaattctct
    12721 tgttcttagg tttagtagga gcagcatatc tcgtgtatgc gctcctttcc caaactgatg
    12781 acaaagaaga tgacaacaac gatgaaatga aattttagga ggagaaataa tggacgaact
    12841 aatcacaaaa gtagagcagt gggctaaaga caagggactg gatcaagcag atccaaaagc
    12901 acagtttttg aaagtagctg aggaattcgg ggaaatagct tcggcgatgg caagaagtaa
    12961 tgatgaacta tttaaagata gcgtaggaga cgttatcgtc acgctgatta tcctttccat
    13021 gcaaaaaggg acaaacgtac aagagtgttt agaaatggca tacaacgaaa tcaaaggacg
    13081 cacagggaaa atggtagatg gtgtattcgt gaagtcgagt gatttggagg gcagcaaatg
    13141 ataaaaaagc tcattcaatt cagcatggat ttatatgata tcgaatcagg agcaacacta
    13201 tctgtggaat cggaccatct aatcataaat tttggtggaa agcgccagat tattttgtgg
    13261 gtagttgatg atgtactgtt tccagaaatt gttcatgatt tcgaagaatc aaaagcggtt
    13321 gagtttgaaa tagtgaaaaa agtaatggaa ttgattgaaa aatacgagga ggacagcgaa
    13381 tgataccgaa gtttagagcg tgggataaac gaaagaacgt aatgagagat gtagccgtct
    13441 tgcattttac taaaaacggc aaaacaaact ttattgaata ttggataaat cctaccgaat
    13501 tgaaatcata tcacgtgcga aacatcgacc tcatgcaatc cacaggaatg aaagataaga
    13561 atggtgtgga tatatttgaa ggggatatag tattagtcag cgtgcgaaat ggcttcgatt
    13621 acttagataa taaagtttgt attgtcaaaa attcaataga ttattccgga ttagtttgtg
    13681 ccactgttga tgaagactta gagtatcaaa tttttaacac agagctgttt gaagaataca
    13741 cgtatgaagt catcggaaat atatacgaga atagcgagtt attggaggta gagtgattat
    13801 cttagctact gactacatcg attctttaaa agatgaaaat gggaatgtcc cagcattgct
    13861 aaattgtgga attaaaagca agaatagaat cattttgtta cggatatcgg ctgatttagc
    13921 aaaatatctt tatctgagca agacagcagt atattacact gcggctacac gttatcagta
    13981 caagggaaaa acaatcagtc aggattatta tgatcgttta atcagtcctg atatgtatgg
    14041 gaattcagaa tcagcaatta agctgtttgg cgcaatagag atatatgccg atgattttcc
    14101 aaatttatgg ttgaaggagg aagcggaatg aaatacgaaa taccactaag tgaagcagac
    14161 gtccagtcaa ttattaacgg tcgggaggtt aataaaaaac ttcctgatgg tactgaatta
    14221 gtcatcagac aaagttattt gaaagatatg gcagctccag tattaattga tcgttttaac
    14281 gtgactgatt ctgtggtaga gaaccacttg aaagaatttc gatcaagtat aaacgacact
    14341 ttcagattag ggagttgatt gacaatgaac accagacacc gcagagtagc aaaactaaga
    14401 aaacaggaac tgaatgtact aaagacaaag tttgaaaaag aatatggaat ttcagcagaa
    14461 gaaacatata aagtggcaag tcagtgtgtt gctgatgcaa gtgatgctat tcgtaagttt
    14521 ggaatttcga tattaagtga tgatcgtaaa tgggaggaaa aagaatgaaa ctaaaagacg
    14581 gattttacgc tagcagtcac ggtatcggcg gtttaatgct agatatgccg acaaagaatc
    14641 ctaaaacacg taagaaacca aaagtcaaag tcggtgacat ggtccgctgt gaagcagagg
    14701 agttcattta tccatttcgt ggatatgtag aacacgtcta taatcactca gcgatcattc
    14761 gcattgaaaa cacgatggaa tgtgataaat ggacagctaa aagcaaagag aatttagcag
    14821 tagtgagatt ggtggatatg gaactaatca atgacaaata aaaaagccgg atcgctccga
    14881 ctaacataat aaaacagaca agtttattat atcacataaa aggagcagtt tgaattgatg
    14941 caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag
    15001 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt
    15061 attacggata tgccgaaggc accgaagcac ggcaataagg cagaggatgc attgattcag
    15121 atgatggata tagaagcgga gagagacgcg attttagcgg ctttgatggc tcttagtctg
    15181 attagtcgtc agatactcta ctacagtttt tgtgtgccag acagcttctc aaactacaga
    15241 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaaatc ggaagctcta
    15301 atagagtttg cagaagcata taaacacgga agaataattg cttataaata gggggaccat
    15361 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
    15421 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
    15481 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
    15541 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
    15601 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
    15661 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
    15721 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
    15781 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
    15841 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
    15901 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
    15961 attatctatc atccgttgct gtttattgtt acatacagat cgcttaggcg gtctttttat
    16021 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
    16081 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
    16141 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
    16201 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
    16261 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
    16321 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
    16381 tgttcaaatg aaggcgaagt attcgattgt agaaatgcta agagaagcta caccaaaaga
    16441 gattgatcag tataagttgg ttcttattgg cattggttgg tttaaggatg aacatattca
    16501 aacaaacatg aagaggtggt tgagatgaag ttatattatg ttgaaaaagc aaattgttgg
    16561 gtggttgaag gctgtctgtc aaagtacgct acagatatta tatctactca attttcagag
    16621 ttgggattga aggttatctt ttgtggtgag tttgtcagtg tgaaagaagc ggtattgcca
    16681 atgacagcaa agagcagacg ctcgcgtggt tcaagtt
//
LOCUS       DAYYAJ010000063        16185 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000063 DAYYAJ010000000
VERSION     DAYYAJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.063"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..901
                     /locus_tag="ACHATZ_001688"
     CDS             44..901
                     /locus_tag="ACHATZ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8404214.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1016..1936
                     /gene="pstC"
                     /locus_tag="ACHATZ_001689"
     CDS             1016..1936
                     /gene="pstC"
                     /locus_tag="ACHATZ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8404215.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1936..2820
                     /gene="pstA"
                     /locus_tag="ACHATZ_001690"
     CDS             1936..2820
                     /gene="pstA"
                     /locus_tag="ACHATZ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8404216.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2835..3641
                     /gene="pstB"
                     /locus_tag="ACHATZ_001691"
     CDS             2835..3641
                     /gene="pstB"
                     /locus_tag="ACHATZ_001691"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8404217.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3657..4415
                     /gene="pstB"
                     /locus_tag="ACHATZ_001692"
     CDS             3657..4415
                     /gene="pstB"
                     /locus_tag="ACHATZ_001692"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8404218.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4428..5105
                     /gene="phoU"
                     /locus_tag="ACHATZ_001693"
     CDS             4428..5105
                     /gene="phoU"
                     /locus_tag="ACHATZ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8404219.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5682..6374
                     /locus_tag="ACHATZ_001694"
     CDS             5682..6374
                     /locus_tag="ACHATZ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8404220.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6371..7138
                     /locus_tag="ACHATZ_001695"
     CDS             6371..7138
                     /locus_tag="ACHATZ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8404221.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7238..8806
                     /gene="liaX"
                     /locus_tag="ACHATZ_001696"
     CDS             7238..8806
                     /gene="liaX"
                     /locus_tag="ACHATZ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8404222.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9103..9423
                     /locus_tag="ACHATZ_001697"
     CDS             9103..9423
                     /locus_tag="ACHATZ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8404223.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9423..9773
                     /locus_tag="ACHATZ_001698"
     CDS             9423..9773
                     /locus_tag="ACHATZ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8404224.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9940..10878
                     /gene="hprK"
                     /locus_tag="ACHATZ_001699"
     CDS             9940..10878
                     /gene="hprK"
                     /locus_tag="ACHATZ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8404225.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10893..11723
                     /gene="lgt"
                     /locus_tag="ACHATZ_001700"
     CDS             10893..11723
                     /gene="lgt"
                     /locus_tag="ACHATZ_001700"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8404226.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11767..12792
                     /locus_tag="ACHATZ_001701"
     CDS             11767..12792
                     /locus_tag="ACHATZ_001701"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8404227.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12807..13745
                     /gene="galU"
                     /locus_tag="ACHATZ_001702"
     CDS             12807..13745
                     /gene="galU"
                     /locus_tag="ACHATZ_001702"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8404228.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13870..14310
                     /locus_tag="ACHATZ_001703"
     CDS             13870..14310
                     /locus_tag="ACHATZ_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8404229.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14313..14915
                     /locus_tag="ACHATZ_001704"
     CDS             14313..14915
                     /locus_tag="ACHATZ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8404230.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14974..16080)
                     /locus_tag="ACHATZ_001705"
     CDS             complement(14974..16080)
                     /locus_tag="ACHATZ_001705"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8404231.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5255 a         2820 c         3495 g         4615 t
ORIGIN      
        1 aaatgataaa ataaacatac tttaggatta aaggagattt ctaatgaaaa aacttaaatt
       61 attcttacca ataataggat tagctattct tttatcaggc tgttcaaagt ggattgatcg
      121 aggagaatcc attacggctg ttggctcttc tgctttacag cctttagtag aaacagttgc
      181 tgaaacttac cagacagaaa atcctggaaa attcatcaat gtacaagggg gcggaagcgg
      241 aactggtttg tctcaagtac agtcaggagc agtcgacatc ggaaactctg atttgtttgc
      301 ggaagaaaaa tcggggatcg atgcctcagc attagtcgat catcgagtag cagttgttgg
      361 gatcactcct attgtgaaca aggatattgg cgtaaaagac atcagtatgg aaaacctgaa
      421 aaaactattt acaggcgaga tcaaaaactg gagtgaagtc ggtggaaaag atcagccagt
      481 agttatattg aaccgcgctt caggaagcgg aacccgtagc acgtttgaaa aatgggtatt
      541 ggacggagaa acagctgttc aggcacaaga acaagattca agcggaatgg tgcgccagat
      601 cgttgccgac acacccggtg caatcagcta tgttgctttt tcttatgttt ccgatgatgt
      661 agatacgttg aatgttaacg gtgtagaacc gacagatgaa aatgtgacaa cgaacaaatg
      721 gacgatttgg gcatatgagc atatgtacac aagagatcat ccaaaaaaac tgacaaaaga
      781 atttttagac tatatcttat ctgatgaagt acaagataac atcgttggag aactaggtta
      841 tatccctgtt tctcatatga aagtcgaacg tgattggcaa ggaaatatcg ttaaagagta
      901 atctttacac aaaatttaca aacttagcta aacttttaca tgttattaaa accaaattga
      961 tgttcaattt gtacactgag tgagtatgtt aaacctgatt aagggggagt catctgtgga
     1021 agatgtgaaa aaagtattat taacaaaatc aaaacgttca aaaatggaac agcgtggaaa
     1081 attcatcagt tttttatgta tcgcgctgat cgtgttggtt gtattatcta ttttttattt
     1141 tgtagctagt aaaggattgg ccaccttttt tactgataag atcaatgtat ttgattttct
     1201 gtttggtaca gattggaatc caagccaaat cggagcaaat gggaaaccaa tggtaggggc
     1261 attgcctatg atcacaggtt cgtttattgt gaccttttta tctgctatcg ttgccacgcc
     1321 atttgccatt ggtgcagcag tattcatgac ggagatctct cctaaaaacg gaacgaagat
     1381 cttacagccg gtcatcgagc tgcttgtagg tattccttct gtcgtttatg gttttatcgg
     1441 tttatcagtt atcgtgcctt ttgtcagaag tatttttggc gggactgggt ttggtatctt
     1501 agctggtaca tttgtgttgt tcgttatgat cttgccgact atcacaacga tgactgttga
     1561 tgcattaaag gcagtgccac ggcactatcg ggaagcttca ttagcgcttg gtgccacaag
     1621 atggcaaaca atctacaaag tcgtgctacg agcagccgtg cccgggattt taacagcagt
     1681 cgtttttggt atggctcgtg cgtttggtga agccttagct atccaaatgg tcatcggtaa
     1741 tgcagcattg atgccaacag gtcttactac tcctgcttct acattgacgt ccatcttaac
     1801 aatgggaatc ggaaatacaa tcatgggaac agttgaaaat aatgtgttat ggtcattagc
     1861 gctgatcttg ctattgatgt cattattctt taatatcgtg atccgtatga ttgggaagaa
     1921 aggagagctg aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg tgttttatat
     1981 atagtttcgg ggatcatcgt attgatttta gcagctttac tactttatat cttagttcgg
     2041 ggaataccgc acatttcatg gtccttcttg accgaacctt ccaaagccta ccaagaaggc
     2101 ggtggaatcg ggatccagct atttaactcg atttatttgc ttttgatcac gatgatcatc
     2161 agttttccta tttctttagg tgcgggtatc tatctttcag aatatgctgg aaaaaactgg
     2221 ctaacagatg tgattcgcac gtcgattgaa atcttgagct cgttgccttc tgtcgttgtc
     2281 ggtttattcg gtttcttggt tttcgttatt caattccagt atggtttctc gattatttct
     2341 ggtgcattgg ctttgactgt attcaacttg cctcttttga ctagaacagt tgaggagtct
     2401 ttacaagcgg tccattacac acaacgagaa gcaggattgg ccttaggttt gtcacgctgg
     2461 gaaacagtga taaaagtggt ggttccagaa gcgacaccag ggattttgac tggtgtgatc
     2521 ctaagttctg gaagaatttt tggtgaagca gctgcattga tctatacggc tggacaaagt
     2581 gcacctgcgt tagattttac gaactggaat ccattaagtg tttcaagccc gatcagcatt
     2641 ttccgtcaag ctgaaacatt agctgtccat atttggaaga tcaatacaga aggcacgatg
     2701 cctgatggtg tttctgtttc ggctggcgct tctgccgtgt tgattattgc tgttttactg
     2761 ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac ttcagcctaa
     2821 ggagaagaag caaaatgaaa aaatataact ggaatgaaac aaatgtcatc accatgccaa
     2881 aagagaaaga agtagcacta tatacggatg atctacatgt ctggtatggc gacaatgaag
     2941 caatcaaagg agtggatctg gaatttgaaa aaaataagat cactgcactg attggtcctt
     3001 ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg aatgaatgat gggatcgcta
     3061 atacgaaaat cactgggaaa atcatgtatc aaggcgtaga tattaatgcg cctcaagtgg
     3121 atgtttatga gatgcgcaaa cggattggca tggtttttca acgtcctaat ccgttcagca
     3181 agtcgattta tgacaatatt acatttgctt tgaaacagca cggcgaaaaa aacaaaaaga
     3241 aattagatga aatcgttgaa acaagcttga aacaagctgc cttgtgggac caagtaaaag
     3301 acagcttgga caaaagtgcc ttggctttat ctggcgggca acagcaacgt ttatgtattg
     3361 ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga cgagccggca agtgctttgg
     3421 accctatttc gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa gactatacga
     3481 tcatcatcgt gacgcataac atgcaacaag cagctcgtat cagcgactat actgcctttt
     3541 tctatttagg aaaagtaatg gaatatgaca aaactaacaa gatatttacc agaccaaaaa
     3601 ttcaggcaac cgaagattat gtgtccggtc attttggtta agacggaggc ctcaacatga
     3661 caaaagagat tatttcatct aaagatcttc atctgtatta tggaaagaaa gaagcactaa
     3721 aagggatcga tctaagcttc aatcaagggg aaatcactgc gatgatcggt ccttctggtt
     3781 gcggaaaatc cacttatctg cgttctttga accggatgaa tgacctgatt ccgaatgtaa
     3841 cgatcaccgg aagtgtttta tataaagaca aagacatcta tggtccaaaa acagatgttg
     3901 ttgaactaag aaaagaaatc ggcatggtat tccagcagcc aaacccattt cctttttcta
     3961 tctatgaaaa tgtcatctat ggattgaaat taaaagggga aaaagataaa aaagtccttg
     4021 atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg ggatgatgtg aaagacaaat
     4081 tgcataaaag tgctttatct ttatccggtg ggcagcagca gcgtgtatgt atcgcacgtg
     4141 ttttggcagt taatccggaa atcatcttat tggatgaacc aacaagtgcg cttgatcctg
     4201 tttctactgg gaaaatcgaa agtatgctgt tagaattaag agagcggtat acgatgatca
     4261 tcgtgaccca taatatgtct caagcttcac gtatttcaga taagacggca ttctttttag
     4321 atggtcactt gatcgaatac aatgatacga agaagatatt catgaatcca gaaaaacaag
     4381 agacagaaga ttatatttca ggtcgatttg gataaggagg aaacggaatg ctacgtacac
     4441 aatttgaaga agaattgctg aatctccata atcagttcta cgaaatgggg atgatggtga
     4501 gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca cgataaaatc ttagcccaag
     4561 aagttatcga aaacgatata aatatcaata acatggaaac acgcttggag aagaaaagtt
     4621 ttgaaatgat cgcattgcaa cagccagtta caactgattt gcgtatgatc attacggtca
     4681 tgaaagctag ctcggattta gaacgtatgg ccgatcatgc ggtgtctgtt gctaaatcca
     4741 cgattcgcgt aaaagggcag acgagaatac cggaaatcga aaaagagatc tccgatatgt
     4801 ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc ttatgtcaaa acagacgaaa
     4861 aagacgcacg tacgatcgcc aatatggatc aaagagtcaa tgactatttc aatcgaatct
     4921 ataatgcaac aatcaaaaat atgcaggaaa atcctgagac agtcatcagt ggaacagact
     4981 acttgaatgt tgccagttat ctagaacgga tcggagacta tgtgacaaac atctgtgaat
     5041 ggatcgtcta cttggctaca gggaaaatca ctgagttaaa tacgaaccac aatgagattt
     5101 tctaaactga agctgtgatt atgccgtttg tttaggaaaa gaggttgtga aatcagcggt
     5161 tgtgaaaaag ctgttctgca tcaagtaata agaacagtca aacaaaaaat agctcctcat
     5221 atttttcgtt tggctggact tattaccgca gatgcaagct tttgaacacc gtttattcgg
     5281 gaaagggttt gtgacaaaac atgtcacaaa cccttttcat catttcatag aaaggataaa
     5341 gaatgcaaaa aaaaattagc atcatcacta tattttttat ttgtgtgctt acttcatggg
     5401 ttattcagca tcaagccgct ttatatgaag tacctgtcat cgagatagaa aaagtacgaa
     5461 taaaagagga tcatcaattg attacaggaa aactgatgaa cacggatcaa aaaggagaat
     5521 cgatttattt atcggttcct tatcaaaaaa atcaaataga aacagcaaat ataaaagcga
     5581 gacaaatgta tctagtccaa atgcatggag atgaatggca gctagtagca aaaaaaaatg
     5641 atggctggct tttcttcttc acaagtatct tcattggcgc catgttgctt attggtggaa
     5701 aacagggtct atttgcttta ggaagtctca tcatcaatat cgttttactt ttacttttgt
     5761 tgctcatctt tactaaaaca gaagggattt ctctattaaa tatatctatc ctgttcgtcg
     5821 ttatagggat gattattagc atattggctt tagatggatg gaatagaagc agtataatca
     5881 aaatttcagc tgcagttacg agtgtttttt tagcattttt tatttgtctt ttgacaatga
     5941 atcattggaa agaccaagga ctgcgctatg aggaactgaa ttatctgaca cgaccgtatc
     6001 gttctgtttt tctttctagc gtactgattg gaacagctgg aggaacactc gataccgtta
     6061 tcacagtcat tgcgacatta gaagaattga ctaagcgaca accagaaata acagcgaaac
     6121 agttatggct ttcaggaagg caagtgggtc gagatgtgat tggctcgatg accaatatcc
     6181 ttttgttcgt ctatatcagt gggtcagttc catcgcttct tttatattta ggaaatcaat
     6241 ggtcgttcaa agaaacgata gaacagcatt tatcattgga atttttacgc gtcattgctg
     6301 gcagcttagg aatagtatta tcgatcccta tctcggtcct cttttttctg actgtaagga
     6361 ggctgaaacg atgaatgtac ttattatact tgttgggatt ttagcaatca gcgtgctact
     6421 tattggcggt acgcaaggta tgtatattct tttaggctta ttcatcaatt tagggatctt
     6481 tttcctgttg ttattcggtt atcatcagaa gtggccgata ctggcattgt cggtcatagg
     6541 atttttattg attgctgtgg ttatcttatt ttttatcaac ggttataatc taaaaatgcg
     6601 tgcagctttt gtgagtatac tgatcttttt attttgtttc ctactattgc ttcctatcac
     6661 tgatttttta gcaatccaag gttttacatc ggtagaacta gaagaattat ctggcttaga
     6721 taagacttta gcgattgatt ttagattact tacgagatcg ttgcttctga ttagtcttag
     6781 tggtgctgtt ttagatgcga gtgtagcaat ttctagtggg acattcgaag tatatcaggc
     6841 aaatccgcaa ttatcttttt accaattgcg ccattctagt ttcgctattg ctaaaaaaat
     6901 attggcgtca acagtaatga cacttctttt tgcattcatg ggaagttctt tagcgttgat
     6961 cctctggttt attgatttag acattccttt tcagcaagtg atcaatgaaa aatcatttgt
     7021 tctagaatat acgatggcta ttcttaccac attgtccgca ctacttgttc ttcctttaac
     7081 ctgcgtgact acagcatatc tctttacaaa aaagaaagaa caactcaaga tggagtaaaa
     7141 gttacaactt aagtcctatg acatttttac tgaatcagtg cataatatgg atgtaaggaa
     7201 aagcttgcaa acaattttgc ttaaggaggc aatttccatg aaagaaagag aacgtatttt
     7261 agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat tagaaagcat
     7321 ggcaactgaa aaagacgaaa agcaaatcaa aaaagaagca gacaaggtaa ctgctagtca
     7381 taaagaaaaa gatcaggcaa gtcaattgat cgataaactt gaaaacggcg aagaagaaat
     7441 ttctgagcca gttgatccaa aggaaagaga aagacaagat caagaaaact tagaaaagat
     7501 cttagatgaa ctagcaactg aagcgaataa aacttcggcg cgtctagatg aagtaaatgc
     7561 tcaattcgct gacaataagg cagcacgcaa tgagaaacag gaagcattga tgcagctgaa
     7621 tacaaaagaa gaattaggcg agttgacaga agaagaatta gcacagcgtc agacgttaga
     7681 agcagaaatc aaagaattag aagctagtgg cgatacactg ttagaagaac aattgaaatt
     7741 agaagcagaa ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag aaacgtttac
     7801 tgataagttt gaattgccag atgattggaa agatcaagca acagatacac tgaatcaagt
     7861 aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga aaacattcca
     7921 aactgtttca gaaacagtga atgacaacat ggaatggaaa gatgtcagct tacgtgtccc
     7981 tgggattgcg acaacgaaat ttgagcatga attttactat gaagctcctg cagcaagtat
     8041 ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca gcaacgatgt
     8101 gaaagttgaa gctaagatca aactttacgg aaaaatgggt gctgaaccat ttgaagcatt
     8161 ctctgaacgg agccagattg aggttaatga agatcatatc tccttccaaa ttccaaataa
     8221 acgtgtgcgt gcagaccttg ttttctactt acctaaacga gtatacgacc acgcagctat
     8281 taaacttcta aatggcaaca tcatgattga aacattggaa gctaaagata tttatacaaa
     8341 atctacgaat ggtaacatca tcgtggacca attaactgca acgatgctgg aagtagaagg
     8401 tgtaaacggc aatatcgata ttcgaaacgg gaacatctta gattctatta tcgaaacagt
     8461 aaatggtaca gtgacatttg gtgcgacagc agaaaacctt tctgtgtctt tggtaaatgg
     8521 tgatgtacgt cttacgatta aagaagataa tttgaaaaaa gtagaagcaa gttcagtgaa
     8581 cggaaatgtc aaagtcgctt taccagatgg catcggtttg gaaggacatg ctaaaacaag
     8641 tcttggaagt atcaacagcc gtctgtccaa ttacgaagta gttcgtgaga aaaaagaacg
     8701 gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt gaaatcgcgc aagtgcaact
     8761 ttcaacgaca acaggaagta tctatttaaa agatacagat aaataaagag gttgtgaaag
     8821 agatgttcag ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc tattttacaa
     8881 aattgtgcct tattgccgat agtctatctc ttgaacaccg tttaccaaga gattatgaac
     8941 ctgccaatta tcgccgagtc acaaggaata atttcagaaa aagctcgtca cgttttatga
     9001 atatggtaat ttgtgaccga gaattttgca gaaagacacc gattaagaag aaggttgtca
     9061 caacgatttt cttcatttaa aatagtagaa cagaggtgag acatgatgaa aaaaaagttg
     9121 acaaaatcta ataaaaatgt tgttttgaca gggactttag caggaatagg agaatacatc
     9181 gggattgatc cgactgttat ccgtgtgctt tatgtattcc tgagcttagt tgcttttgga
     9241 agtccaatta ttttatacat tttattagct ttgattatcc catttgcgcg ttctgcacga
     9301 acaaactatg gacataacaa tgcatattat gaaaaaaaca attatcgtga acaaactcgt
     9361 gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt
     9421 taatgtcgta ctttcaacgt attttagtta atacgttaac attcatatca ttggctgttc
     9481 tttttcctgc gatggtccat gtgagcagta tttggacggc cgttcttgcc agcttcgtat
     9541 tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat actgtccctg ccctttacat
     9601 tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacctcct
     9661 tttttgtggg agcagaacat tttagttttt cttcattcgg tgcagcgttg ttagtagcta
     9721 tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa cagtagaaaa taaaagaaag
     9781 ctgtgccgaa ctatcggcgc agttttttta tttagaatca ggaatgcgtt ttctattaca
     9841 ttggtaatta aataaaaacg ttttttttaa ttggtagtac ttttggctag acaatgataa
     9901 aatgatagag aacactctaa tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt
     9961 agccaattag tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa aagcttagac
    10021 cgtgtcatcg taactgggga catctcacgc ccaggactag agttgactgg ttattttaat
    10081 tattattccc ataatcgttt acagctattt ggcagtaaag aaatcagttt tgcggaacgg
    10141 atgatcccag aagagcgttt gatcatcatg agaagattat gtagtgaaga tatgcccgca
    10201 tttattattt caagaggatt accagctcca gaagaaatga tccaggcagc aaataaaaat
    10261 gggatagctg tactgcgttc tcccatctca acttctcgtt tattaggaga attatccagt
    10321 tatttagatg gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt ggatgtttac
    10381 ggattaggtg tactgattca aggtgacagt gggataggaa aaagtgaaac agcgttggag
    10441 ttgatcaaac gcggccatcg attgatcgca gacgatcgag tagatgttta tcagcaagat
    10501 gaattaacgg tgatcggaga accgcctaaa atcctggaac atctgattga aatccgagga
    10561 atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg ctctatgcaa
    10621 gtccagttgg ctgtttatct ggaagcatgg gcaaaagata agaaatacga ccgtctgggc
    10681 tctgaagaca cgaccgttga aatcgcggaa gtgggcattc cacaaataaa aatccctgtc
    10741 aaaacaggac gaaacgttgc aatcatcatt gaagtagccg cgatgaattt ccgtgcacgt
    10801 acaatgggct ttgacgcaac gaaaacattt gaagaaagat taagtcgctt gattgaagaa
    10861 aactcaggca acgactagca aggagtgaca atatgttagc acaaatcaat cgagttgctt
    10921 ttgagcttgt agggattcct atctactggt atgcattgat catcgtatca ggtatcatta
    10981 tcgctatgtg gcttagcagc agagaggctg ttcgggtagg attaaaagca gatgatgtga
    11041 cagattttat gttgtgggga ttgcctatct ccattattgg cgctcgctta tattatgttt
    11101 tatttgatct tccccagtat atagctgatc caatccaaat attcaatatc cgttcaggag
    11161 gcttagctat ttatggcgga ctgattgcag gcgcgatcac gctgtatttt tttactaaat
    11221 atcattttat ttctttgtgg acttttttag atatcgctgc accaagtgta ttattagcac
    11281 aggcaatcgg tcgttgggga aatttcatga accacgaagc atatggacca gacacgacgc
    11341 gaagcttctt agaaagtctg catttaccgc aattcatcat tgataatatg tatatcgaag
    11401 ggatctatcg tcaaccaaca tttctttatg aatcactttg gagtttgatt ggatttattt
    11461 tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc cttttatgtt
    11521 atctaatgtg gtatagtttc gggcgtttct ttattgaagg attacgtaca gacagtcttt
    11581 actttttagg tgtgatccgt atttctcaat tattatctgc tgtgttattt gtcggtacat
    11641 tgattctatt tatttggcgc agaaaaaaag gaaatttacc ggattatgac cgctcattag
    11701 gaaaaaatca acaaattatc tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt
    11761 ggtaaaatga attatagagt agcggtttta ggaccaggct cttggggtac tgctttggca
    11821 caagtcctcg ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc ttcacaagta
    11881 gaagaaatca acacccaaca cacgaatcaa cgttatttac cagatcatta tctacccgat
    11941 tccatccaag cattcaccaa tatggaagat gcagtaaaag acgcagatgc ggtgctattc
    12001 gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa attgaataca
    12061 aagccagtga tcatccatgc aagtaaagga ttagaacagg gaacacataa aaggatctct
    12121 gacatcttgt tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt tctttctgga
    12181 ccaagccatg cagaagaagt agcaaaacat gacatcacta cgatcacagc tgcaagtaca
    12241 gatgcaaaag cagcaaaata tgtacaagag ctgttcatga ataattattt tcggatctat
    12301 accaatcctg atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat tattgctatt
    12361 ggtgctggcg ctattcacgg cttagggttt ggcgataatg caaaggcagc aatcatgacg
    12421 cgcggattag ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt
    12481 atcggattaa gcggaatagg cgatctagtc gttacttgca caagtgttca ttctaggaac
    12541 tggcgcgcag gcaacttgtt aggacaaggg cagcccctag aagaggtgct agccaatatg
    12601 ggaatggtcg ttgaaggtgt gaatactacc caagcagcga tggaactagc ccaaatgatg
    12661 gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc
    12721 aaaacagccg caaaagaaat catgttacgt gatggaaaaa aagaaaatga attctcatta
    12781 acaatttttt gaaggagcga atgtatatga aagttaaaaa agcagttatt ccagcagcag
    12841 gattaggaac aagattttta ccagcaacaa aagctatggc aaaggaaatg ttgccaattg
    12901 ttgataaacc aacaatccag ttcatcgttg aagaagcgtt gaaatcaggt atcgaagaca
    12961 tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga tcactttgat tcaaatattg
    13021 aattagaaac aaacctaagt gaaaaaggaa agaccgaatt attgaaatta gtagaagaaa
    13081 cgacagatgt caacttgcat ttcatccgtc aatctcatcc aaagggttta ggacatgccg
    13141 ttcttcaagc aagagccttt attggaaatg aaccatttgt cgttatgcta ggcgatgata
    13201 taatggaaga cgaagtacca ttgacacagc aattaatcga tgattacgaa cgtactcatg
    13261 cttctacgat cgccgttatg caagttcctc atgatgaaac ttcaaaatac ggaattatca
    13321 atccaggaga agtattggaa gatgggttat acaatgtcaa aaactttgtt gaaaaaccag
    13381 atccaagcaa agcaccaagt gatttagcta ttattggacg ttacttattg actccagaaa
    13441 tttttgatgt attgtccgag caacaaccag gagccggaaa tgaaatccaa ctaactgacg
    13501 caatcgatac attgaataaa acacaacgcg tatttgcccg taaattcaca ggtaaacgtt
    13561 acgatgtcgg tgacaaattt ggttttatga aaacaagtat tgaatatggt ctaacacatc
    13621 cagaagtagg tgcaccttta cgtgagtaca tcctgtcttt aggtaaaaca ttagaagcag
    13681 aaggaagcaa gatcgatcat aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa
    13741 aataaccctt ttttctaacg agcttcagct gctaattgaa taaaaagtag ttgaagctcg
    13801 tttttatttg tttaaaaaac acgaaaatgc tatccttata gaaaagagag acaagaaggg
    13861 agtttgatca tgtcaggagg agaaattgca ggattgatcg cagcaatcgc ctttgcgata
    13921 cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta aaactttaga aaaagtagat
    13981 cagactgtag cagaagcaaa tacgacgatc gaaatcgtca caaaagacgt agatcttttg
    14041 tctagacaag tagaaggctt actggtcaaa agtaatgaac tgctcttgga tatcaatgga
    14101 aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg atttaagtga aagtgtatca
    14161 gaacttaatc attctagtaa aaacatcgcg acgaaagtcg gcggaatcgg taaaacgaca
    14221 gctaaagcga cggttgccgg aaaagtcggt gggacagcga tgaaattttt taaaaacaaa
    14281 aaacatactg aaacgacagg aggaaaataa aaatggctaa aaaaggtgga tttttattag
    14341 gtgcagtagt cggaggcaca gccgcagcaa tcgccgcgtt attgttagca ccaaaatctg
    14401 gtaaagaact tcgggatgaa ttagctgccc aagcggatga tttgatggat atggcttcag
    14461 attacacaga tatggcgaaa gaaaaatcga ctgagttaac aggtattgca aaagaaaaag
    14521 cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag tgtcaaacaa aaagtaagtg
    14581 aatcagcaga tgaagcagga agtgaatctg acgatatctt gttaaatctt aaagaacaat
    14641 cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga tatgaaaaaa cctgcagatg
    14701 aattaggaag tattgctaaa gatacttcag aagatatcat gattgatgta aaagacgctg
    14761 ctggtaaagc aggagatact gtcaatgaag gaatgtcaga agcaaaacca gttgcagatg
    14821 actcaaaaga aatgatgaaa atggcaaaag aagatgcgaa agaagctaaa aaagaaataa
    14881 aagatacaat gacagattca tctacaaatc actaaaatca caaatttata gaataaaaag
    14941 gtgggagcgg actagctctc acctttttta ctcttatttt tctaaaacaa gtaattcttt
    15001 tggtgtagtc gtgaatggca cacagccatc gcttgtcaca tatacacagt cttcgatacg
    15061 tacccctact ttttcaggaa tatagattcc tggttcaaga gagaagcaca tcccttcttc
    15121 gattactaag tcatttcctt ctacaaggga agggaattcg tggacggttg ttccgatccc
    15181 gtgacctaaa cggtgattga aatattcgcc gtagccatag ctgttgatca cgtttcgtgc
    15241 gatttgatca agttcacctg ctgtcacacc tggacggacg gcttcttgtg cggcaagctg
    15301 tgcttcaaga gtgatgttgt agattttttc ttggaagtcg gttggttttt gataagcaat
    15361 tgtacgagta gcatcactgc aatagccatt ccaaacaacg ccaagatcga ataacacaag
    15421 ttcattcggt gaaatcttcg tatttcctgg tgtaccatga gggcttgcag catttgctcc
    15481 tgcaaggact aatgtgtcga aagacatttg tgaaacacct tgttttttca attggtattc
    15541 aatttctgca atgatcgctt gttcagtcac gccttctttc actgctttaa agccaatctc
    15601 aaatgcaaca tcggcccatt taccagcttc gatcagtttg tctttttcct ctagagtttt
    15661 gatcagttgt agacgttgga tgatggaagt tagatcagtg taagaatccg ttcccgtcaa
    15721 gatatcagag atagcatcga agcgctcgac agtcaaggcg ttcttttcaa tccctacatt
    15781 gccaggctga ccatagcgag cacgaagttc ttttccgatg atctcccaag ggttttcgct
    15841 gtctaaatag ccacggacat catacgccca actactgttt tgagcatctt caacttccaa
    15901 agcaggagtg aaaaggaaag gatcttgttc aagagggata aaaagagcta gtacacgctc
    15961 atgaggatca ctttcgtatc cggaaaagta agcgatgtgg ccagggtcgc tgatataagc
    16021 caattcgact tggtgttgtg ccatccattg acgtagttca ttgattttta cgttattcat
    16081 ttggcaacct actttctaaa ttaattttgt attcaataat tgtaccactg atttattaaa
    16141 agaccaacgt tctaaaaact gaaaaaagta aaaattttca tgaaa
//
LOCUS       DAYYAJ010000064        15974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000064 DAYYAJ010000000
VERSION     DAYYAJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.064"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..878
                     /locus_tag="ACHATZ_001706"
     CDS             210..878
                     /locus_tag="ACHATZ_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8404232.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1078..1398)
                     /locus_tag="ACHATZ_001707"
     CDS             complement(1078..1398)
                     /locus_tag="ACHATZ_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404233.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1435..2817)
                     /locus_tag="ACHATZ_001708"
     CDS             complement(1435..2817)
                     /locus_tag="ACHATZ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8404234.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2973..4160)
                     /locus_tag="ACHATZ_001709"
     CDS             complement(2973..4160)
                     /locus_tag="ACHATZ_001709"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8404235.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4167..5198)
                     /locus_tag="ACHATZ_001710"
     CDS             complement(4167..5198)
                     /locus_tag="ACHATZ_001710"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8404236.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5244..6668)
                     /locus_tag="ACHATZ_001711"
     CDS             complement(5244..6668)
                     /locus_tag="ACHATZ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8404237.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6843..7748
                     /locus_tag="ACHATZ_001712"
     CDS             6843..7748
                     /locus_tag="ACHATZ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8404238.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7839..8516
                     /locus_tag="ACHATZ_001713"
     CDS             7839..8516
                     /locus_tag="ACHATZ_001713"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8404239.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8513..8683
                     /locus_tag="ACHATZ_001714"
     CDS             8513..8683
                     /locus_tag="ACHATZ_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404240.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8797..9693
                     /locus_tag="ACHATZ_001715"
     CDS             8797..9693
                     /locus_tag="ACHATZ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8404241.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9748..11118)
                     /locus_tag="ACHATZ_001716"
     CDS             complement(9748..11118)
                     /locus_tag="ACHATZ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8404242.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11356..12102
                     /locus_tag="ACHATZ_001717"
     CDS             11356..12102
                     /locus_tag="ACHATZ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8404243.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12099..12938
                     /locus_tag="ACHATZ_001718"
     CDS             12099..12938
                     /locus_tag="ACHATZ_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8404244.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12939..13888
                     /locus_tag="ACHATZ_001719"
                     /pseudo
     CDS             12939..13888
                     /locus_tag="ACHATZ_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14029..14781
                     /locus_tag="ACHATZ_001720"
     CDS             14029..14781
                     /locus_tag="ACHATZ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404245.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14800..15951
                     /gene="queG"
                     /locus_tag="ACHATZ_001721"
     CDS             14800..15951
                     /gene="queG"
                     /locus_tag="ACHATZ_001721"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8404246.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5187 a         2992 c         3219 g         4576 t
ORIGIN      
        1 acaaccgtaa cggttgtgta aaaatatcat tttagagcat aaaaaaaagg agtggatttc
       61 tccactccaa aaaagtctaa cttttggggg tcactacagt gtcacagtcc tttttttagt
      121 aaaaggatac atttcttcct tgtatattcc atgcgtgttc gtgagaaagc atatataatt
      181 taattaacag atagttaggg ggagaaaaaa tgattttttc aattacaatg aaactggtgt
      241 tcgggttgat cggtttatta ttagtggtac gtttgcttgg gaagaaatcc atgtcagaaa
      301 tcacaccgtt tgatttagtc tatacgcttg ttttaggtgg gatcttagaa gaatcaacct
      361 atgatgacaa tgtccatgta gggcatgtcc tatttgccat tgcgctttgg gcagtaatga
      421 tttatggtat cgaacgtatc gtccaaaaaa atgaaaaggt caatcgttgg gtcaaaggcg
      481 agccatctgt tctaatcaaa gacggcgtca tcaacatgac agagttgaca aataaccata
      541 tcgagatgga gcagttgcga gcaatcttaa gacagcaaga atgctttttt cttgaaaatg
      601 ccaaacatgt tatcctagag aatgccggtc aaatgagtgt attgaaaaaa tcagatgaag
      661 acaaagcttt atcgatcctg cttgtagatg aagggcaaat tcagcataaa gtgcttcagt
      721 ccaatcaatt gactgaagca tggcttatgg agaacttgaa gaaagaaggc tatgcagatg
      781 taaaacagct gatttatgtt gaatggtcag aagaaaaagg gctctacata ctaaataaag
      841 aagacgtcat gcatgaatct taccgaatag atggatagat agacagaggt tgtgaaatca
      901 gcgccttggc ttgttactca aaactaagcg actttttcac aacctctgat atctaatata
      961 cgttgttcca gaagttgctt cttcgaaaaa taagccaaaa ttccacaaaa atttaagaag
     1021 caattttcga aaaatccttt cttatttttt gaagttgatc acttctgtca caacctctca
     1081 tgtcacaaac catttttttg tttcctttaa ctcaatatac cgtacgtctt cccacaatat
     1141 cggctgatgg tcaatcataa agtaatcttc ataggcttca ccaatgaagt gcccttcgac
     1201 ctcatccaag agtcttccaa attcatcttt taaattgagc tgaatgatta ctgctttacg
     1261 agataaaaag gcttctgtca gtatactatc gatttcttgc aatgtcattt gttgtttagg
     1321 aagaggctgt ttgcgtgctt cttcttcatt tttttgatac cgttgactag ttcttccatc
     1381 gcaaaagcag tcccccattt taatccaaat ggccgatctc gatactcgtt atagtcaatg
     1441 aatttttttg ctgtccgtct catcttttcc accttctaat ccactcatac cacctgcatg
     1501 cccgccaacg agagaactac gtgcgatggc gcgtcctcct tgcaagagac tatgcgcatg
     1561 gatgattgat tgaaagccgt atttttttct aatcgtatcg acaacaaaat caactttcgc
     1621 ttcatttatc tgcttttcag gatcttcaaa aaggttaagc tgtaaacctg tcgcgtatac
     1681 gaggtcggaa cagttcacgc ctacacgccg aatatcctgg tagtggtaat gctggtggaa
     1741 aatggtcaat aaagcatctg caagatctct gctagcattt gtcgcttcga ttttcatttg
     1801 ctggtgaaac ccggtcttgc catttggatc aagatagcct aaagagtatc ctacccataa
     1861 gctgactact tgtgtttttg ctgcagatcg tctcaagcga gtgccgactt ggtcggccat
     1921 ttctttgata acgatctcga tttcttctcg tcttgtataa tctctatcta ggatttgact
     1981 atttccgata ctttttgatt ttggtgtata ttcttgtccc aaaaaacttc gatcgatgcc
     2041 ccaagcatgg gcataaagct gagtaccgat gacccctagc ttgcttttca agtgataata
     2101 atccgcgtga gctaaatcat aaacagaatg tatacccatt tgtttcagac gttttgctgt
     2161 tcgtttgccg attccccaaa agtcagtgat atctgagatt ttccataact tttctggtac
     2221 atcttcataa cgccattcag caaacatttg ctttccgtct tttgagccat tgtctaaggc
     2281 tagtttagca agtagcgggt tatcgccaat tcctatagtc gtgtagattc cggtctgctc
     2341 aaacacatct ttatggatca gtttagccat gtcgtacgca tttgttttac caaagagtgc
     2401 catgctattt gttacatcaa ggaagctttc atcgacggaa tagacatgat ggttcgcttc
     2461 gtctgcatat tttttataga tagaattgat ttctctgttc ttttccatat aataggccat
     2521 ccttggggca acaatatata aatcacttgg ataggggaag ggaagatctc tggcacggct
     2581 gatattggtg atgccgtaag ctttttttgc tgcaggactg gaagccaaga ttaatccgct
     2641 accccgtgtt tctgtcgaat cggacggata ggacatgact actaatttag ttgtaagggg
     2701 atcaagatcc ttttcaacgc actctacgga ggcgtaaaaa gatttgcagt caatacataa
     2761 aatatctctt gaaggttctt tttcataaac aaataccgga ttttgattcg cccacataac
     2821 aatcacctct tcaacacaat tagaacacac gttctctttc cgtgtcaata aaaaacagca
     2881 attttcaaat acgagcaaaa ggctcacatt tggaaattgc tgagaggtta tttttatcta
     2941 tttgttttta gctgagatcg ttgatttttt tatcaaacat gaacagcttc tggatcgatc
     3001 tttgttgtcc catttccaat gtgtgcaaca taaaattccg gagcatagcc gacaactttc
     3061 atgtattcat catagacatt gtttttgaag gcaggtatat tttcttcttt gacaagagca
     3121 atcgcacagc caccaaatcc agctcctgtc attcgtgcac ctaagacacc ttcttgtttt
     3181 tgagcagcat ctactagtgt atctaattcg atcccagtta cttcataatc atagcgaaga
     3241 gagtggtgag aatcatttaa cagttgacca aatgcttcta agttcccagc ttctaattca
     3301 gcttttgcct tcaaagtccg ctgattttca gaaacggcat ggcgagctct gcgtatcaat
     3361 gtatcatcgc cgatttggtc aatagttgat tcaaattctt cttcatctaa gtctcctaaa
     3421 gaagagatag gcaaagtttt ttgcaaacgt gccaaggctt cttcgcattc gctacggcgt
     3481 tcattgtatt ttgaatctgc caattcccgc cgcttattcg tattcatgat gacgattgca
     3541 taaccatcta gtttcactgg aaccatttcg tatttcagcg tatttgtatc taataagatt
     3601 gcatgatcta gctcaccgaa gccgatggca aactgatcca tgatacctga attgacaccg
     3661 atgaattcat tttctacttt tttacctgtt tggactagtt ccaagcgatc tgtttttaaa
     3721 tcaaacagat cttctaatac aacacctact aataattcta atgaagcgct actggaaagt
     3781 cctgcaccat tagggattgt tccttccact aataactcaa acccatgatc gattgagtgt
     3841 ccagcttctt ttaatttaag gaccattcct tttacgtaat ttgcccagtt tgctttttta
     3901 tcatattcta gctcatctaa agtaaaacta atgacaccga tatcgtcaaa gtttgtggaa
     3961 taaactaata ttttattatc gtctcgaggt gctgctacac cttgcgttcc ataagtgatg
     4021 gaagcgggga agacatggcc tccattgtaa tctgtgtgtt caccaatcaa attgatgcgt
     4081 cctggtgaga agtattgtgt agttttttta ggtccgaatg tttcggcaaa ttttgtgttt
     4141 aagcttcttt tgatttccat gatgagttat tcctttcttg ttttgatttt gaattctgtt
     4201 actgtttcaa atactgatcc ggcttttaaa tgaatcgaac caaatgctga gaagcgctct
     4261 gcacctggag ctgtttgagt ctcaaatgtg atgccaccgt gatgtgccag ttttcggttg
     4321 cgcatttctg gtgtttctgt gccgaaattt gcagtgaaaa taaccacact agaagcatct
     4381 gtagcaatat ctacttggat tcttttatcg gggctagtca agcgagcggc ttctttaccc
     4441 aagccggttt cttttaagaa aaatggatga tcgattccgt caacaagatt tttttgatcc
     4501 aaatcactag caaaaacgga cgctaatttt ttaggtattt gaaaatcgaa agcacttcct
     4561 gcagcatttt cttttacccc tataggaata ctatctgttc gcaaaggagc ataagcttcg
     4621 ctattgagcc aaagctcatg ttgatcgatt gactgagaag catctcccgt caagttgaag
     4681 taaacatgat ttgtcggatt gaataaagta tcttggtcac tggtgccgcg agaggtcact
     4741 cgccagatat tatcttttgt caaagtatag cggatttcca catccattgt acctggaaaa
     4801 ccatgttctc cgtcgggact tgtagtagta aaaatgacag aagcttcatt ttcgccattc
     4861 aaaatgacat aagaccattt ttttaattca aatccaggag caccaccatg cagattatgt
     4921 ccagtttttg gatctgtggc taattggtaa gtttttccat tgagagaaaa gcggccattt
     4981 tcgattctcc ctgcggtgcg accgatagaa gcaccgatat acggatcttt ttcgatatat
     5041 tcttcagcgg tgtcaaatcc caaaactaat tctcgatcat ttgattttaa actgacgatt
     5101 ctagcaccta aatcagagat agaaagtgct agcttgtttt tgttctccaa ggttatcaag
     5161 tgataacctt ggccaaaatc tttttctgta atcttcattt tcaagatcct ttcttaatgg
     5221 gaaggaacta ctgcatcgtg aagttaatct tccattgtat cagctggagc caatttgttt
     5281 tccaatttct caacgatttg tgcatgtttt ttctctgtca atgtaatctt ccaagcaaaa
     5341 ataatagcgg agaaaatcat caaagcagca ggagcataga atgcataagc tttgaaagtc
     5401 gttacgccat gagcagtgat atcagcagca gtagcattac cagtcatccc agaagcgata
     5461 gcgacaaacc ctacgatccc gttagacaaa gcaccagcga ttttatctag taaagggcga
     5521 attgatagag taacagcttc gttacggata ccatttttcc attgaccata ttcaacagag
     5581 tctgtgatcg tcatcaaagc ggataagaaa atcaattgtt gagggaagta gaacaatact
     5641 agcccgatga taacgattgg caaacttgaa cctgcgatcg taaagaaaaa gtaagaagaa
     5701 atcatcattg cgatccctcc aaggtaaacg tagcgacgag agatgacgcg tgtaagcatt
     5761 gggaatagtg gaacagctac taaaccaagt aaggcagcaa ctagaccaac gatccaaaat
     5821 tcagttggtt ggccaagtac atatttgaag aagtagaata atacggcagt tgttgctgca
     5881 ttggcaatcg cataagcgat ataagcaagg gcaagccaca tcaactgatc attttgagca
     5941 actgctttga acacatcttt gatcccagct ttttcatttt gctgacggat cgcagtatcg
     6001 acttcttttg tgcccaaggc agtgataatc gcagtaccgc ctgaaataac agcagcaatc
     6061 acaccgaacc agaaccagcc tgaagcgcct tcaatatgtt tatgagtaac aagataggta
     6121 aagaaagtaa cgatagggac aacgactaga gtagtaccat ttgcacctag agaagagccg
     6181 aaacgagcaa acgtagcaaa tttttcacgt tcctttgttt ccgtactcaa agctggaatc
     6241 attgaccaaa aagcgatatc cttaaatgaa taaaaactat ccaataagat gaaaacgatt
     6301 gcaaataata tgataaataa agtcgtatta tgagtagcta agccaaagaa attggtaaag
     6361 attgctacca agagtacgga actaacaacg ccgccgataa ctaaccatgg tttgaattta
     6421 ccgtattttg tacgagtatt atcgatgatg ccaccaatca aaggatcaaa aataatttca
     6481 actaagcgga tacctacaac taagcttgta actgtagcaa tcatttttgc attcgttgcc
     6541 ttatctgttc ctgcgaacat ctggcttgtc acgaatacca taaaataagt gcttaatgta
     6601 acatagaatg tgtcgtgtcc aaaagcgcct aaagcgtagg acacacgttg gatcattttg
     6661 cttttcataa tctgtattcc tctcctgttc aatatataat actaagtaaa ccggttacaa
     6721 cgaataacaa agatctttta atgacttaaa cttaacaaga aatcgcttcc tttttaatat
     6781 atttctgtga cttttattta actttttgtt gcattatact aaaggagtaa ggagggatag
     6841 acgtggagca atcgattttt ttaaaaaagg aacatcaaca agtagaaaaa gaattgtatt
     6901 ttgatttttg tggtttctct aaaacgatgc cgtttcattc ttttggtcct gctattcgca
     6961 atgattacat cctgcatgtc gtgatggaag gcaaaggatc ttatcatgtg aaaaatcaac
     7021 aattccaatt gaaaaaaggt gatctgttct tgattcgtcc aggagactcg acgttttact
     7081 tatcagatgg agaagatccg tggatgtatt gctggctttc atttggtggg agtgctgcag
     7141 aagaaatcat tcggcagtcg cttttcagag aagaccaata cacgatggtc tcttctgaca
     7201 tacaaaaata tatcgacatt attttagaat gcatgaaaga tacggaagaa accttggaaa
     7261 atgaattaca gttaacagca ttaacctacc gttttttatc agtcctttta aaagatggcg
     7321 gaaaggtcag cggtggtgta caaaaggttc attcctcttt agctgttgaa acagtcaaat
     7381 acatcgaggc acattatgct gaaaaattgt ctgttgagga aatcgcccag catttagcgg
     7441 tcaatcggag ccatttgtcg cgagtattca aaaatcatat gggtaccagt atcaaggaat
     7501 atttgatcgg ggtacggatc aatcgagggg cgtttcttct ttctctgacg gatgaatccg
     7561 tggaaagtat tgcctatcaa gtagggttca atagtctagt cgtatttagc cgaatgttca
     7621 aaaagagtac cggagaaacg gctacgaact atcgtaaacg tatgaaaaac gaaaatttta
     7681 agaatattgc tttggatacc ttgaaaaaac agttggaaaa acaaaccgca gtttcttgga
     7741 caacctgatt agtctggtta aaatgacaag catcggcaaa agaaacttgt ggcggttatg
     7801 ctacactgta aagcgaagac taaaaaggag gaaaagggat gcgtacactt atcatcatta
     7861 gccatccaac gatcgaagaa tctttaaatc agcaattttt caaagaagct tctgctggtt
     7921 taccagctac ttggcatcat ttggaatcaa gttatccaga cggaaaaata gatatagaga
     7981 ctgaactaca gctcttggcc cagcatgatc ggatcatttt ccagtttccg ttttactggt
     8041 atagttctcc tgctcattta aaaatttggc aagataatgt cttggaacga gcagcacatg
     8101 tattaaaagg gaaagaacta ggaatcgtat tgacaacagg tgtggcggaa aaagaatacc
     8161 aagctggagg aaaagaagaa tatacgattt ccgaattttt gaggccgtat cagcgaatcg
     8221 caaataaatt ccatatgacc tatcttccgc cattcgtttt agcacaattt atgtatttat
     8281 cccaggaaaa acgttgggag aagctaattg cttatcagca gtatctctca ttggaaggca
     8341 aaccatcgct gactcagcgg attgactggt ttattcaaag agtacaggaa aatcagaaaa
     8401 tgcaggaaga agaatctgaa aaacaagcat atatcgtaga agcactgact gaggcaaaag
     8461 agcagatcga agacttgacc tttacactgc aagaaatgaa aggaacttca ttatgacaaa
     8521 tgattggctg atccaacaat tagatgaatt aagcgaaaaa tcagaatatc aagaccgtgc
     8581 gctcttatat gaaataaaga aactgatggc tgagatagac gaacgaacaa tgcagctgca
     8641 aggagaaatc gacggacaat cttggaatca tctcaactgg tgaaattttc ttataaataa
     8701 caaagataaa aatgtgcaca gcgacaatta tttgctacaa taagaatatg aagcgtttga
     8761 taacgctatt atacataaaa attggtggtg aaaaaaatgt atgatgaaaa tgagtgggga
     8821 gaaaatgttc ggatgacccg aagaagacag caaaaacagg cagctcgtaa gcggatgatc
     8881 atgattgggg cagcagttgc cgtgtttttg atcatagcag gaggagttgc gggtctatat
     8941 tttcatggaa aaaattcgtc agaagcgaaa agtacgacca taacggaaac aacgacaaag
     9001 aaaacagaga cgaaacaaac aagtgagtca tcaactaaaa caaaagagac acaaacttct
     9061 gaagaaaaaa cagcaaatgg tttgccagag caacttcaag caatcgtaaa tgattattcg
     9121 aaaaagatcg aagaaaagac gcctgcattg attgaagaat accagacaga aattcaggga
     9181 aatcaagagg gaatagctgg tttatcagca gttgctaacc aaaaagcacg tgagcttcaa
     9241 gcaatctccg acgaagggat cagaaaatta cgtgcagcgt atcaggcagc agaaaataaa
     9301 gacggtgtag acttagatac tttgatcaat caattatcgg ctaattatac gaatcatgtc
     9361 gctaagattt ccgatattta tttgcagact agcgcctctc ttcaagcaga gtcaacaagc
     9421 acacaagaca caacatcttc tgattctgag acagcagagt cggtagaagc ttctgaaatg
     9481 caagctcgaa caacacaaga cacaacagat tcctctgaaa ctaataccaa cagcgcgact
     9541 tcacaggctg cgacaaccgt agttttggaa ggagaaggtc caaatcaaat agccgaacga
     9601 actggcgtac cagtagaaac gatcttgtcg ctgaatggga tgagcatgga gaattatttc
     9661 ctgactcccg gtgagacgct aaaattgaat tgataaaagc aataaaaaag aacgaagctt
     9721 ccactaaggt gaagcttcgt tcttttttta ttttccggtt cttttttcgc gtacaaagta
     9781 atggatcgtc atgtaaagca agaaaacgag tccaataatc aatgagattc tcgtttcagg
     9841 attgataaac atgaaaatca atacgatagc taaaaaagca agtgtcacat aattcgtata
     9901 aggagatagt ggcattttaa aaggatgatc atgcatttta tctgcgtgct tctttttgaa
     9961 gcgaatctcg ctgatcaaaa tcactaccca aggaaccata ccaggcaaaa tacttgagct
    10021 gtatacgaaa acgaaaatat tttctgactg gttgatgaac attggtagaa gataatttaa
    10081 gatcagacca attaagattc cacctgagat tgctacgaca ggataaaaag gcgcaccgtg
    10141 acgagatagt ttcagaaatt ttttagacat ctttccattt atggcaagtg tatagaccat
    10201 acgacttgcg ctgaaaatcc ctgaattgca acctgataat gccgctgtaa tcacgacaaa
    10261 attaatcagt cctgcagcaa atgtaatgcc gactttagaa aaagtttgta caaaaggtga
    10321 accaatctca ctcaattgat tccacggata gatactcaca atgataaaaa tagcaccaat
    10381 ataaaaaatc aagattcgac cgatagtgga ctggacagct tcaatgatcg tctttttagg
    10441 attctctgct tcaccagcag tcattccgat cagttcgatt ccttgatagg aagcaacaac
    10501 gatcgataat gcaaagaaaa atcctttgac gcctccagtg aagaatccac catttttcca
    10561 aagatttgaa atccctactg cctgaccatg attaccaaat ccaaataaga ttaccccaag
    10621 acctgcaatg atcatcaaaa tgatcgttat gactttgatg atcgaaaacc aaaattctaa
    10681 ttcgccaaac gccttgacag aaataagatt tgccagcata agaaacaaga cgacaatgat
    10741 tccgggaata attgccggca aatcaggcca ccaataattc atataagatc ctacagcaat
    10801 cacttcactt attcctacga caatatattg aaaaatatta ctccaagcgg tcaagtagcc
    10861 agccacagga tggatgtact cgctcgcgta atttgcaaaa gaaccagtcg aaggatcaac
    10921 gtaaagcatt tcgcctaaag cacgcataat caaatacaaa aagaatcctg caatcgcata
    10981 ggctcccatc actgagggcc ctgtccaacg tatggttgag gaggcaccca taaacagacc
    11041 gacaccgatc gttccaccta gtgcgatcat ttggatatga cgggcactaa gacttcgagt
    11101 taacttttct tcttccatac aaaaactcct ttcgttgttg agtcaacaag aaaaaataat
    11161 caaacgtttc aacacatgtt tttcattatt aggttagcct tattgaactc ttgatgtgat
    11221 tctacagaaa attttcataa aatgcgagga aaattcgaaa aaaacttttt catcattcta
    11281 cttgaaaatg ctttccattt tagttaaact atatttaaat aaaaagttag gtaaacctaa
    11341 aaaggagtgt aagatttgga acgtacggcc attgtaattc aagacctttc agccgcttat
    11401 caagggaaaa ctgttttgaa aactatcaac ttgacgataa atccacaaaa aattaccggt
    11461 attatcggac caaacggtgc aggaaaatca acatttatga aatctttgct agagttgata
    11521 ccagctacta ctggtaaagt caccttttct ggacagtcgg taaactcagt ccgtaaaaaa
    11581 atagcctacg tggaacagcg aagtgaattg gatctttcct ttccaattga tgtattaggc
    11641 gttgtacttt taggaacata tccatcttta cgaattggac aaagacctgg gaaaaaagaa
    11701 aaagaacgtg caagacaagc tttgaaaaaa gtagggatgg aagaatatgc caaaagacag
    11761 atcagcgaac tatcgggtgg acagcttcag agagttttta ttgcaagagc tctagcccaa
    11821 ggagcagaat ggatcttttt agatgaacca ttcgtaggga ttgatgcgtt aagtgaacga
    11881 aagatctttg acatcttgca ggaattgaag aattcaggaa aaacgatttt gatcgtccat
    11941 catgatcttc ataaagtaga agaatatttc gatgaggtta ttcttttaaa taaacagctg
    12001 atcgcttccg gtccagtaca agaagtcttt acatcgaaga accttcaatt ggcctatggt
    12061 gagatcatca gacatttagt aaaaggagga gagaaaaaat gatccaatcc tttattgatg
    12121 gattaatgaa ttatcaattc ctgcaatacg ctttggtcac ttccatgctt gtaggacttg
    12181 cttctggagt catcggttct tttattattt tacgagggat gtctttgatg ggagacgcta
    12241 tctctcacgc agttcttcca ggtgtagcca tttcttatat gtttggtttt agttacatct
    12301 ttggagccac tgcttttggg atgctaacag cagcagtgat tggtttcgtg actcagcgga
    12361 gccgattgaa aaatgataca gcgattggaa ttgtctttag ctcctttttt gcattaggga
    12421 ttatcttgat ttcatatgcc cagagtgcga cagatttgta tcatatttta tttgggaatg
    12481 tcttagctgt tagggaaagc gatttgttgc tgacagcact cgttagtgga atcgtcttga
    12541 tatttgtctt tttcttttat aaagaattga agatcacatc attcgatccg acaatggcaa
    12601 aagcttatgg gctgaacaca tcgctgatcc attatctttt gatgttcttt ttgacattag
    12661 tggctgtagt cagtttacag acagtaggaa caatcttggt gattgccatg ttgatcacac
    12721 cagccgccac ggcttacttg ctaacgaacc atttactgaa aatgatcatt acagctgcag
    12781 gaatcggtat gctaagtgca gttgtcggtg tgtttttcag ttatagttac aattggccat
    12841 caggagctac gatcgtgtta gcatgtaccg cattttttat ccttgctttt ttattttctc
    12901 caacaaaagg aattttattt cagaaaggaa gcagatagat gaaaacaaaa aaatcactct
    12961 ttttaatctt ggcagttagt ttcttggtat tagccggttg cggaaagcag gcttctgacc
    13021 aagcggatga aggaagtaaa gaaaagttgt cagtcgtggc taccaattcg atcttggcgg
    13081 acatggcaaa agaggtcgga acagatcaaa tagatatcca cagtatcgta ccagtcggaa
    13141 cagatccgca tgaatatgaa gtactaccag aagacatcaa aaaggcaagc gatgcagatg
    13201 ttattttata caacggtttg aatcttgaaa caggtaacag ctggttcgat aacttgatgg
    13261 aaacggctaa aaaagaagaa ggaaaagatt attttgcagt tagcaaaaat gtagaacctc
    13321 tttatttaac tagcggtgaa gaacatacaa aagcagatcc ccatgcttgg ctagacctat
    13381 ctaacggaat aaaatatgtg gaggaaatcg cacgtatatt ctctgaaaaa gatgcagaaa
    13441 atgcgacact ctataaaaaa atgcagaagc atatgtggaa aaactaaaag aattagatac
    13501 acaagcgaag gaaagttttg cttctatcga agagaacaaa aaattattag taacaagtga
    13561 aggggcattc aagtattttt cacgagcata tgatttgcca gcagcttata tatgggagat
    13621 caatacagaa agtcaaggta cgcctgatca aatgaaagcg atcattgatc agataagagc
    13681 aaaagaagta ccggttttat tcgtggaaac cagtgtagac tcaagaagca tggaacgggt
    13741 agcaaaagaa acgggtttga aaatctatga taaactgttc actgattcca tagcaaaaga
    13801 aggagaacaa ggagattctt actatcagat gatgaaatgg aatattgaaa cgatccatga
    13861 aggtttgtca caaacaaaag aaagctgaaa agtgcaaggt ctagcttgag gagcagacct
    13921 tgcatttttt ataagagata tgaaaaaaaa ttatgctgtt tttcgtcgta cagatacctt
    13981 ttttgaagaa attccgttat agttagagta aggaaggaag gtgtggctat gaattttaca
    14041 cgtaaagata aatatacgat taaagaagaa gtctttcgat tgatcagcca gcaggaaaat
    14101 accgacaagg atcaagacct tcaatttctt gaagcagaat tagctaaatt attcatagca
    14161 aatcaaaatg agacaacaga accagatgaa gagctgtaca taggcaaatg gttgtttgat
    14221 gaatttcagc ctttagatga aaataccggt atcggttatt ttgtgttcct ttctccagtc
    14281 ggcttcacac ctggcgtatt agttaaacaa aacgacgaaa taaccgtcaa agcatggcct
    14341 gcattatttt cagtcatcaa agcagaagga gtaatcaaaa agatccagtc ggcctattat
    14401 aaaagtcaat ttattgccaa cagcaaatta atgaatatgt tttataatcg tctagtagaa
    14461 attgctaggc ctgggaaaat cccgcaaaca ttcacgtaca cacagcaaaa aaatctctat
    14521 gaagaagtat tgtattcccg cgatctgtct gagttttcgc catgggaaga ggtaaggctc
    14581 catgaactgt taagtacaaa accagtttca ttgaattttt ttaatgcatt agataacaaa
    14641 agaaaattaa tttcaacaaa ttattgggaa gtatttgctg caagaaaaaa atggatggaa
    14701 gaaaagaaga ttgccgatca gcagatccag tttgtccagc tgaaaaaaga aaaactatat
    14761 tatgggttat acagccgtta acagcaagcg aggaagacca tgacattaaa agaagaaatc
    14821 atacaagaaa gcaaacgatt aggaattgac aaaatcgggt tcaccacggc ggaacctttt
    14881 gattcattaa aagaatcgtt ggaagaacaa aaagcagctg gccatacttc tggctttgaa
    14941 catccaaata tcgatgagcg tctttatcca gaaatgacat ttgatcagcc gaaatcgatc
    15001 atttccattg cacttgcgta tcctactagg atccatgaag aagtgcctag ggatgaaaaa
    15061 cgcgggcagt ttgctcgtgc ttcttggggg atcgattacc atgatatact cagagaccgt
    15121 ttgaaccgat tgatcgaatt catccaaaca agagcagaaa aatggcaaaa agaagaggaa
    15181 tggcgtctag ctccgcaagt agatactgga gaactgatcg atgtcgctgt agcacagcga
    15241 gctggtttag gatttattgg acgaaacgga ttgttgatca ctgaagagtt cggttcattt
    15301 gtctatcttg gtgagatcgt tactaatatt gtatttgagc ctgataaacc aggagaattt
    15361 ggttgcggtg actgtatgcg ctgcgtgact gcttgtccga caaaagcatt gcttggtgac
    15421 ggaagaatga atgcccagcg atgtttgtct tatcaaactc agacaaaagg gatgatgccg
    15481 gaagaatatc gtaaaaaaat gcgaaatgtc atttatggat gtgatatctg ccagttagtc
    15541 tgcccttaca atcaaggaaa agattttcat tttcatgaag agatggaacc aaaagtagac
    15601 gaaatatatc caaaattaaa gccaatgctt tcgatgtcta ataaagactt caaacagcaa
    15661 tttgggcatt tagctggttc atggcgtggg aagaaacctt tgcagagaaa cgcattgatt
    15721 gctttagcta atctaggtga tcgaacggca ttgcctgaga tttcagaatg tttaacagat
    15781 gtccgccctg tcatcagagg aacagcagct tgggctattg gacgtctagg aacaaaagaa
    15841 ccggaaaaat ggcttgatgt attagatgaa atattagcta aagaaacaga agaagtcgtt
    15901 ataaacgaat tggaacatgc ccagaaatta ttgcggagaa agttaaaata aaaaaatagt
    15961 taacattatt caaa
//
LOCUS       DAYYAJ010000065        15902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000065 DAYYAJ010000000
VERSION     DAYYAJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.065"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..511
                     /locus_tag="ACHATZ_001722"
     CDS             <1..511
                     /locus_tag="ACHATZ_001722"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8404247.1"
                     /translation="YKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPY
                     YAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYI
                     EPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKK
                     KRRRTQKFL"
     gene            639..1322
                     /locus_tag="ACHATZ_001723"
     CDS             639..1322
                     /locus_tag="ACHATZ_001723"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8404248.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1631..1704
                     /locus_tag="ACHATZ_001724"
     tRNA            1631..1704
                     /locus_tag="ACHATZ_001724"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1665..1667,aa:Arg,seq:tct)
     gene            1863..2222
                     /locus_tag="ACHATZ_001725"
     CDS             1863..2222
                     /locus_tag="ACHATZ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8404249.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2345..3976
                     /locus_tag="ACHATZ_001726"
     CDS             2345..3976
                     /locus_tag="ACHATZ_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8404250.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4038..4679)
                     /locus_tag="ACHATZ_001727"
     CDS             complement(4038..4679)
                     /locus_tag="ACHATZ_001727"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8404251.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4865..4941
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4961..5269
                     /gene="rplU"
                     /locus_tag="ACHATZ_001728"
     CDS             4961..5269
                     /gene="rplU"
                     /locus_tag="ACHATZ_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8404252.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5296..5640
                     /locus_tag="ACHATZ_001729"
     CDS             5296..5640
                     /locus_tag="ACHATZ_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8404253.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5653..5946
                     /gene="rpmA"
                     /locus_tag="ACHATZ_001730"
     CDS             5653..5946
                     /gene="rpmA"
                     /locus_tag="ACHATZ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8404254.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6380..9187
                     /locus_tag="ACHATZ_001731"
     CDS             6380..9187
                     /locus_tag="ACHATZ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8404255.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9378..9869
                     /locus_tag="ACHATZ_001732"
     CDS             9378..9869
                     /locus_tag="ACHATZ_001732"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8404256.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9972..10961
                     /locus_tag="ACHATZ_001733"
     CDS             9972..10961
                     /locus_tag="ACHATZ_001733"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8404257.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10994..11797
                     /locus_tag="ACHATZ_001734"
     CDS             10994..11797
                     /locus_tag="ACHATZ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8404258.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11818..12732
                     /locus_tag="ACHATZ_001735"
     CDS             11818..12732
                     /locus_tag="ACHATZ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8404259.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12815..13189
                     /locus_tag="ACHATZ_001736"
     CDS             12815..13189
                     /locus_tag="ACHATZ_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8404260.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13328..13573
                     /locus_tag="ACHATZ_001737"
     CDS             13328..13573
                     /locus_tag="ACHATZ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8404261.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13745..14809
                     /locus_tag="ACHATZ_001738"
     CDS             13745..14809
                     /locus_tag="ACHATZ_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8404262.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15055..15480
                     /locus_tag="ACHATZ_001739"
     CDS             15055..15480
                     /locus_tag="ACHATZ_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8404263.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15473..>15902
                     /gene="nusB"
                     /locus_tag="ACHATZ_001740"
     CDS             15473..>15902
                     /gene="nusB"
                     /locus_tag="ACHATZ_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8404264.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLK"
BASE COUNT         5085 a         2634 c         3471 g         4712 t
ORIGIN      
        1 ttacaaaaaa ataactttta taagttctat aacgttgatt atatttttaa ttcttattct
       61 tctcgtcgtc ttggttcccc gaatttttgg tttgactcct tatattattg aggacaaaga
      121 aatgagtcca tattatgcac aaggaaatct gatatatgta cgaccgaaaa atacaaatga
      181 gattttagtc ggagatatta ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg
      241 tcgcgtaata gccgttgaaa acaaccacga agatttttat acaaaagctg atacaaagac
      301 ttatattgag cctggagtag taaaaaagag aaatattatc ggtagtccca tctttcagat
      361 tccatatatt ggactagtcc tttcaaaaac ggcatttgca tggattcagg ggttattctt
      421 tacgattgct ttttgcctaa caggaatcac agcatggaat acctttgtag agttgaaaaa
      481 gaagcggaga cgaacacaga aatttttgta agaatgttaa aaacataaat gtattccaga
      541 gatccaacga ctcttctgaa catgaaacac gagaaaacac tcatgcagct tgtagattta
      601 gtcagttgtt cgtataatgt gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc
      661 gtcctgtagt caaagaagaa ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg
      721 gtgagctagg caaactggaa gaagaagctc atgtagcaga agtaccagtt attccccatg
      781 aaacagttgt atttttgaaa tttttacttg gacagcttca gcctgaacga atcttggaga
      841 tcggagcagc tatcggtttt tcatcaagtg tcatggccac gacaatcgga gaaaacgctc
      901 atgttacgac aattgatcgt ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac
      961 gtctaggact gacagataaa gtgactttgc ttgaaggtca agcagcagat atcttgccgg
     1021 agttgtctgg tccgtatgac tttattttta tggatagtgc aaaatcaaaa tatattgaat
     1081 ttttacctga atgcttacgt ttgttaagaa aaggtggcgt tttgatggta gatgacatct
     1141 tccaaggagg aacgatcttg ctcccagatg aagagattcc ccgtggcaaa cgagctatcc
     1201 atcgtaaatt aaatgaattt cttcgtgttg tgatggatca tcccgatttg acatcgacaa
     1261 tcttgccatt aggtgatggt gtgatcttaa tgacaaagga atcagaaaca atcgagttgt
     1321 gatacatgat tatggcagcc tgtagataat cgattgaatc gagggctcaa ttcgaacttg
     1381 atgtaaaagt ttctatattt gtaagtcaat taaaggatac aggaaatcta aataaacagg
     1441 agtagcatga agtcaatgta gctcctgttt tcaattgata accttagaac agctgaagga
     1501 gaatgtctcg ttttaaaaga ttgatttagt agttgggcca aaataattca ttatttgcta
     1561 attaaataac tatttcgtat tgacagaagc aatttaatta tctataatta ttgatgttcc
     1621 gctgattctt gtcatagtag ctcagctgga tagagcatcc gccttctaag cggacggtcg
     1681 ggggttcgaa tccctcctgt gacgctaaaa aaccgttaga aactgaggtt tctaacggtt
     1741 tttctttgtt ttttgtgcca atattgtttg ttttcaaagg caaataaaat aaaaaaacat
     1801 ccttatcgag tacaattata aggagcaaaa aaataaaact catcagaaaa gaggaagaag
     1861 atatgacgat actatctaga attttagtga ctctagttgc aattgaattt ttttatatca
     1921 tgtatatcga aacggtgaga actgattctg acacgacaag tagggttttt aaaatgagta
     1981 aagaagagtt aagcagaaaa tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg
     2041 gactattagg agtgggacta ctttatggag cttatctttc ttccgcatct aaagaaatca
     2101 caagtatgct attaatatct attttttttg ttgcattata tggcagtttg actagcgatc
     2161 gttggatttt actcaaacaa ggtgggctag ctctgttggc attgctttct attttcttat
     2221 aaatatagaa aaaataagaa tttatctaac aggaaacttt gtgaaaaata ttactttatc
     2281 tcatgaaata tgatattcta atgaatggta ttttagaaaa tttcatgaaa aaggagttag
     2341 ataggtgttc aaaaattatg tttcctcact aaaaaaagaa ttttcaggtt atgatctttc
     2401 aagatttcag aaagatcttt tggctgggat cacagtagct gctgtagcat tgcctttagc
     2461 cttggctttt ggggtatcaa gcggagctga tgcggccgca ggtttgatta cggctatcat
     2521 tgccggtatc gttattggcg gtttatcagg cggtttttat caaatatcag gtccgacagg
     2581 tgcgatggca gctatcttga tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct
     2641 tgctactttt ttagcgggtc tatttttact tgcagcagga cttttccgat taggaacact
     2701 gacttctttt atcccagcac ccgtaattac ggggtttact tcaggtattg ctatcattat
     2761 tgcattaggt cagatcgata atttatttgg tgttcactcg gaaggtacaa acgttattga
     2821 aaaactaagt agttatcaaa cgataggatt tccgataaac acagcctctt tgatcatggg
     2881 agggctagtc attttaggga tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag
     2941 ttcgctgctg gctatcattt tggcaacagc agttatgcta ttgttccatt taccagtagc
     3001 aactgtgggt aagattccac aaacattgat cagtagtaat cgtttgaata tgggagattt
     3061 tagcttttct gccttgcaac aagtggcagt tccagctatt agcattgctt tattaggaat
     3121 gattgaaagc ttgctatgtg gtgcttcggc tggccgaatg gcaaataagc ccttagatag
     3181 caatcaagaa ttaatggcgc aaggaattgg aaatctgttg cttcctttct ttggcgggat
     3241 tcctgctact gcggccatcg cccgtacaag cgtggccatc aaatctggtg cacaaactag
     3301 attagctggt atgatacatg cagttgtctt actgctctcc atgcttgttt ttgcgccagt
     3361 gatgtcacag attccaatgc cagcacttgc aggtgttttg attgtgacgg cttggcgtat
     3421 gaatgaatgg gaaacgatca aggagctatt tactaataaa tattggtcag cagttctatt
     3481 gtttatcctt acgatgattt gtacagttat ctttgattta agtattgcta tcgtgatcgg
     3541 ggtgatcggc ggctgtgtat tttttgttgt caaaagcgct gctatcacga tttcattaga
     3601 aacgatcgat tggcaaagaa tggggctgcc agaaacggaa aagctgaata actggacagt
     3661 agtgtatatt agtggtccac tgttttttat gtcttcagaa aaactcaaaa ataaattgga
     3721 agaactaaat catcacgatg gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt
     3781 gacggcacgt gcagtttttg atgaatttca agaaaaagca gagcaaagag gacaaacgat
     3841 tatctatact tccttgcagc cagaagtaga aaaacaactt tctcacttgt gggaacatac
     3901 attcacgcat caacatcaaa cagttgctca tgcgttgaca gctttacatg cgaaatatac
     3961 tgcaattagc cattaaaaca atgaaaaaga acagacaaga ctgtaaactg aagattttat
     4021 ggtcttgtct gttcttttta atattgctgt aaaagtgaag ctagtacttg ccagtcttga
     4081 tggataacag gtacaaggga gcgattgtag aaaatagctg ctggatgaaa aagtggaaga
     4141 attcgatagt ttttttctga ccattcgtag gcatcctttt gttctgttaa ttgtaagaca
     4201 ggccctgtat agagttgacc atgatttttg gtaaccgtat attctttgcc aagtaaacgt
     4261 tgaagaccaa tgtttccaag tgtcgcaatt aattttggct ggaccttcat cagttcgtaa
     4321 tccaatattg gtgcatgcgc aaatacttcg gaacgagtag gtgtccgatt tggataaaca
     4381 gtttctgact gttgtgtccg tttattgatc cgatgagtca cgcgatacgg gcggctcctt
     4441 acagcactgg tgatatacac ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc
     4501 aattcttttc cagcttgacc gctaaaagga acatgcgtat caatctctgt tttccctggg
     4561 gcttctccca cgagcatcag tttaggatga agaggtcctt gcccagcgat aaatccttct
     4621 acaggacggc cgttcattct taaagaagct tcttgtatca gttcttttgg gtattccatt
     4681 tatgctcctc ctttttcttt tattataaga ataaaagaaa cacaatgcga ctattttatt
     4741 tggtagaact gtgaggatag aaaagcagaa gtattgcttg aatttttgtt ttactttttg
     4801 attttaaatt tagttgacta acggttgctg ttctggtaag ctagtactgt tgtaattgtg
     4861 gactccacag ctacaaccgc tcagaacaag taataagcac agcatatgct gtcacttcgg
     4921 atggcgagtc taagtatatc agaaggaggt gctttatagc atgtacgcaa ttattaaaac
     4981 tggtggtaaa caagtaaaag ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt
     5041 tgaagcagga gaaaaagttg tttttgacga agttatctta gtaggcggag aatctacaaa
     5101 agtaggcgct ccaactgttg caggtgcaac tgttgaaggt actgttgaaa aacacggcaa
     5161 acaaaagaaa gtcgttactt tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg
     5221 tcatcgtcaa ccatatacaa aagtggttat cgacgcgatc aacgcttaat agcgtttgag
     5281 aaagaaggcc tatatatgat caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt
     5341 tcattcactt tgaccgggca tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg
     5401 ggtgtttctg cgttagcgat cagcactgtc aacgggattg cctcattagc cggttttgag
     5461 ccgattgttg aaatgaatga agaagaaggc ggttatcttt ataccgaagt cacttcagga
     5521 atgacacaag aacaaaacaa tattgctgag attctattag aaaatctttt attaggatta
     5581 caatcgatag aagctgaaaa ctcagaatat atccaaatta aaacaattaa tgataaatag
     5641 gaggtgcaga ccatgttgtt atctatgaat ctacaattat ttgcccacaa aaaaggtggc
     5701 ggttctacat ctaacggccg tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat
     5761 ggacaaacag ttacaggtgg atcaattctt taccgtcaac gcggtacaaa aatctatcca
     5821 ggtgtaaacg tgggtatcgg tggagacgac actttatttg caaaagttga tggcgtagta
     5881 cgtttcgaac gtaaaggccg taacaaaaaa caagtgtctg tttatccagt agctcaagaa
     5941 gcttaattga atcttatgat taaagaggtt gtgacaagac ttttgtcaca gccttttttg
     6001 tttataaaaa taaaagttgt agtggaggct acgaagttta ctttgtcttt ttacacttaa
     6061 tatttttttg ccaatttgga atcaggatgt tttgataact atcgttttca attgatggaa
     6121 gctatattgt ttgctatcag tgaaataatt atatggtaaa cagtgttctt tttcttgttt
     6181 ccattataaa aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt ctatctatcc
     6241 gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg aaacagaaat aaatacatag
     6301 tattatgttt tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac cattggcaaa
     6361 acgcattgac cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga
     6421 atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg ttagggattc agcgaacaaa
     6481 tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga cagacggacg
     6541 accagtacgt tacatatatc aaacactcgt tacgcacgta aagccagtta caaaacatgt
     6601 agtcagttac aaagaaacgc cggtctctga agaaaaaaat acactccaaa tagatactaa
     6661 ggatatcttt gcaaaaatca ttggagctaa cggcagcatg aaaaattcag tggaacaggc
     6721 aaaagctgcg attttgtatc cgccaagagg attaaattgt ttgattacag gacctaccgg
     6781 atcaggaaag acttattttg cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat
     6841 agatgaggaa acggaactga ttgttttcaa ttgtgctgat tatgccaata accctgagct
     6901 tttgatgagt catttatttg ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa
     6961 aatggggatt atcgatcaag cggatggcgg tatgctgttt ttggatgaga ttcaccggtt
     7021 gccgcctgaa ggtcaagaaa tgatctttta ctttatggat catggaactt acagtcgtct
     7081 tggggaaaca acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga
     7141 cccaggttct tctctattag aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc
     7201 ggcttttgaa aaacgtcctg caaatgagaa agtcgattta gtaaaaatca tgattgccca
     7261 cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa aagcattgat
     7321 cggcagtgtg acctatggaa atataggaca attgaagtca aatatccagt tagtatgtgc
     7381 acgaggattt ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg atgatttgac
     7441 agaagggatt cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa tggcagagct
     7501 ttctaaaata ttggagccaa agataactgt agcaccaaat gatacgatca tgaaaatcca
     7561 atcggactcc tatgagttac cttataatct atatgatatc atcggagata aagcagcttt
     7621 actgaagtcg gatggtttgg atcaagaagc aatcaatcat ttcatttcta cagatatcaa
     7681 catccatctg aagtctttct ataaagacca tggtttttct tttaatgcag acagcaaatt
     7741 agcagaattt gtagatccga aaatcataga agtgaccaat cagatttatg taatggtcaa
     7801 aagatcttta ccatatgagt ttcagcaaaa ctttatttat gctatgagct tgcatatcag
     7861 ttcttttttg aaaaaaatca acttaggaga agaacggcat acgaatgata acatccgtga
     7921 gatggctttt gattttccag atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga
     7981 aacgtatttt caagtggaga ttccagaaag tgaagactat tacttgaccg ttctactcgt
     8041 ctcgttacgg gcaaatcaag caagcggcag aatcggtgta gtcgttgcag ctcacggaaa
     8101 cagcacagca agcagtatgg tccaagttgt ccaacagttg ttagatgcag agaacgtacg
     8161 agcagtagac atgcctttgg atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa
     8221 tgtacaagaa gtagatgaag gaagcggggt catactctta gtagatatgg ggtcccttgc
     8281 gtcgtttaat tcgcaaattc aaagagacac tggaattcca gtacgtactg ttgatatggt
     8341 aacaacttct ctagttttgg aaactgtacg aaaggtctca gtattaggga cagatctaga
     8401 tatgttgcat gaatcattga aaaattttcg aggttacgca gaaatatctt ctgtagaatc
     8461 tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt ttggctatct gcgcatctgg
     8521 cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga
     8581 aacagaatta gaagttctag ctttgtcgat tgtggaagtg aaagaagaac tgccagttat
     8641 ccaaaaagaa tatcagatca tagctactac tgggattact gatttgaata tccaagcccc
     8701 atttattcct ttagaacgtt ttattgatca aaatatcgaa gtcattttgg atcagttgct
     8761 gatggaatca gaattggagg aaactgaatt catctcccta gatgaagagt cagctaaaaa
     8821 tacgtgtgtt gagtttatct ctgataactt catctttatt aatggtagta agttaatcga
     8881 tccaatgtgg caattttcta cacaaatctc tcagacgacg ggaatcggcg atgaagaata
     8941 tggattcaaa atcaacctag tgatgcacac agcaggaatg atcgaacgta ttattcggaa
     9001 tgaaccattg actgtggaag aaaacgaatt gacaaataca acgaatgatc ctctttatag
     9061 ccaattggca gcctcagttg ttctgttgga agatcagata aaagtaaaag tgccgataga
     9121 agaaatgtat tatctgctac gtctagtcca taatcagtta gataaaaaag aatacactgt
     9181 gccatgaata cacaaaaacg atacactttt atagtgtatc gtttttgtgt attggttaag
     9241 aatcctttaa attcaattag tgtatcgtaa aaaagttggc acgctttttg cttatagtag
     9301 tgtgtgctag gaggtggttg ttcacacaga aatgataggg gtttctgtga agtagtgagg
     9361 aagaaggagt tttgcagatg gatattcgat tggcacgtat agatgatcgt ttaatccacg
     9421 ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg
     9481 atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa agaagctgca ccgccgggga
     9541 tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt ggattattat
     9601 cacgagcaaa agtgatgctt ttattcacca atccccaaga tgttgaagcg gttgtccgct
     9661 gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa
     9721 tgattaccaa ttttgtttca gtaaatcaac aagatattga ttcctttcgt tacttgaaca
     9781 gtcaagaagt ggagttagag attcgaaaag tacctgctga caggaaaatc cttttgatgg
     9841 aactactagc gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag gtcaaataat
     9901 tctgttgtaa tggttagcaa ttattaatat ttttgaattt gttacaacaa aagtaaatag
     9961 gaggtataaa tatggtaggg attatcctag caagtcatgg acaatttgct gaaggaatct
    10021 tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg attttgaaac
    10081 caagtgaagg tccagatgac ttgagagcca aattggaaga agcggttgct tcttttgata
    10141 atcaagacga agtgttattc ttagttgatt tatggggcgg aacaccgttc aatcaatcaa
    10201 acacattatt cgaagagcac aaagataaat gggcaatcgt cagcggtttg aatttaccaa
    10261 tgttgattga agcctacgca tcacgctttt caatggaatc tgctcatgaa attgcagcac
    10321 acatcattga aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag
    10381 aagcaccgaa agcagcagtt gaagacgcac aacctaaagg agcacttcca gaaggaactg
    10441 tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt ctgttacatg
    10501 gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag
    10561 atgcagtatc aaaggatgat ctgcgtaaga gactaatcga acaagctgct cctccaggtg
    10621 taaaagcaaa tgttattcct attagcaaaa tgatcgaagt agcaaaagac cctcgttttg
    10681 gaaataccaa agcattgctt ttatttgaaa atcctgaaga cgtattgaca gctgttgaag
    10741 gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg
    10801 ttgtaagtaa agtattgtct atgggacaag aagacgttga agcgtttgag aagcttgaac
    10861 aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga ttcacgcgat aacatggatg
    10921 atattttgaa aaaagcaaaa gctgaattag caaaagcgta ataaataata ttaaaattta
    10981 taggaggctt atcatgtcta ttatttcaat aattttagtc gtattagttg cctttctagc
    11041 tggtatggaa ggtattcttg atgaatttca atttcatcaa ccactagtgg catgtacatt
    11101 gatcggttta gttaccggta acctcgaagc tggtatcgta cttggcggta cgcttcagat
    11161 gatcgcttta ggttgggcaa atatcggtgc cgctgtagca ccagatgctg cattagcatc
    11221 agtagcatca gcaattattt tagttttagg tggacaaggt gttaaaggtg ttccttctgc
    11281 gatcgcgatt gccgttcctt tggctgttgc agggttgttc ttaacaatga tcgtacgtac
    11341 aatcgctgtt ccaatcgttc atttgatgga tgcggctgct gaagaaggaa acatccgtaa
    11401 ggttgaaatg tggcacatca ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc
    11461 agcagcgttg ctatttatcc cagcacaaac tgtgcaatca ttccttgaat caatgccagc
    11521 atggttaaca gatggtatgg ctatcggtgg gggaatggtc gtagctgtag gttacgcttt
    11581 ggtaatcaac atgatggcta caaaagaagt atggccattc ttcgttattg gtttcgttgt
    11641 agctgcaatt tctcaattaa cattgattgc tctaggtgct ttaggtgttg cattagctct
    11701 tatttacttg aacctttcta aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc
    11761 tggtgaccct ttaggcgaca ttttgaatga ctattaatct tgaaggagga aaataaaatg
    11821 gcagaagaaa aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc
    11881 ttcattcaag gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc attttctatg
    11941 attccagcaa tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc attaaaacgt
    12001 cacttggaat tctttaatac acacccatac attgcttctc caattttagg ggttacattg
    12061 gctctggaag aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt
    12121 aaagtaggta tgatgggtcc tttagctggt gttggtgacc cagtgttctg gtttacagta
    12181 cgtccaatgc taggtgcact aggtgcttca ttagctatgg gaggaaatat ccttggacca
    12241 atcatcttct tcttaggttg gaacattatt cgttgggcat tcatgtggta tacacaagaa
    12301 tttggctaca aagctggttc taaaattact gatgaccttt ctggcggatt gttacaagac
    12361 gtaacaaaag gtgcttcaat tttagggatg ttcgtattag ctgcccttgt agaaagatgg
    12421 gtatcaatca aattcttacc agttgtatca actgtaaaat tagataaagg tgcttacatc
    12481 gaatgggata aattgccttc aggtggtgaa ggaatcaaga gtgcatttga acaagttaat
    12541 aatggcttag ccctttctcc tgaaaaggta acaacattac aagacaactt agaccaattg
    12601 attcctggtt tagctgcatt aggattaaca ttgttatgta tgtggttgct taagaaaaag
    12661 gtttctccaa tcgttatcat ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc
    12721 ggacttttat aatccataaa aatatagctc gggcgttgtc ccgagctttt tttatatagt
    12781 atactgttca taaacaaata tctggaggga gaaaatggtt caatcaatca atacaaaagt
    12841 agacttggta attgatgcta cttcgtttac gggtctgaca gattacgggc aaatcatgat
    12901 aggtgataat ggttttgagt tttaccatgc gcgagataga cgcaaatata tccaaattcc
    12961 atgggaagaa gtggattatg tactagcatc agtcatgcta aagggaaaat ggattcctcg
    13021 atatgccatt caaacgaaaa aaaatggtac atacacattt tcttccaaag aacctaaaaa
    13081 agtgcttcgt gcgatcagag aatatgtaga tgaagataga atggtacaat cattgagttt
    13141 ctttgatgta gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa
    13201 aacttctaag aggaggtttt ctttattttt ttactttgca gaagaaaata tgaaatagca
    13261 aaaccaatta tttgtcccct ttaaatccca tatgctaaaa ttgattagca aagaaggagg
    13321 ttatattatg agttggttat ggacattgat tgttggagcc gtgatcggag caattgcagg
    13381 tgcgatcacc agtaaaggaa attcgatggg ctgtgtaacg aatatcattg ctggactagt
    13441 cggttcaagt attggacaag cgattttcgg cgaatgggga ccaaaagctg caggtatggc
    13501 acttattcca tctattttag gcgcagtgat tttggtagca gttgtctcat ttttctttgg
    13561 aaaacgatca taagttttta caaaatagta aactaaaagg agttgcagcg agactgtgac
    13621 tcctttttgg ctttatttat ttaaaataaa aagaagtttt ccggaaattt ttatttaact
    13681 gtcgtgtacg aagaaaagtt tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt
    13741 cattatgatg ctacgtgtag aaaaattgcg caaaaaaatg caagaagaaa atttggattc
    13801 atttttggtt accagtccgt acaatcttcg ttacttaacg aactttacag gtacgacagg
    13861 attagctgtt attactttgg aaaaagcatt ttttattact gattttcgtt ataccgagca
    13921 ggcggcagcg caggctcaag ggtttgaaat tattaaaaat gtcggaccta tttttgatga
    13981 ggtagcaaac ttagtacgca aagaagaact gcactcatta gcttttgagg aaacaacagt
    14041 ctcattttta gaatattcgg ttttagaaga aatcatcgat gcggaattag tacctgtttc
    14101 acagatgatc gaagaactca gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa
    14161 agcatgtcat attgcggata tggcatatga tcacatcctg aaaatgatcc aaccgggcat
    14221 gactgagatt gaagtcgcaa atcaactaga cttttatatg cgttctcttg gcgcttcagg
    14281 tgtttctttt gatacgatcg ttgctagcgg attgcgttcg gctatgccgc atggtgtagc
    14341 aagcaaaaaa atcatcgaac aaggtgactt gatcacattg gactttggtt gctattacga
    14401 agggtacgtt tccgatatga cacgtacatt cgctattggt gatcctgggg aaaaattaaa
    14461 agaaatttat caaatagtct tagatgctca attagcagtt attgatgctg caaaaccagg
    14521 aatgactggt gtccaattag atgcagttgc tagagattat atttcaaaac atggttacgg
    14581 ggaagcattt ggacattcta caggacatgg gatcggttta gagatccacg aaggaccaaa
    14641 cgtttcttct cgtgcagaaa agcaatttgt accaggaaat gtgatcactg atgaaccagg
    14701 gatctactta ccaggaatcg gtggagtacg tattgaggat gacttgttga tcacagaaga
    14761 tggaaatcgt gtgttgactc gttcaccaaa agaattgatc atcttatagt ttcatcaata
    14821 gaagaagtaa gcgtaaaaat cagtgttcac agactttgtt atagaaaatc actcgattta
    14881 atgatacact atagaagaac ttttaagctg taagagataa taaagttggg tgttttctcc
    14941 aattcatagc agtttgcagc tgatcggcgg cagttttctt accacgtgat cactatgccg
    15001 catcatagag aattcggatg tatctcgtgc ttaggcaaag ggaggaataa ataaatggca
    15061 gatgaaaaaa atttgatttt aaacgcaaac caagatttag gagagatcgt gatcgctcct
    15121 gaagtcatcg aagtaattat cgggattgca gcttctaaag tagaaggcgt atacggaatg
    15181 cgcggaactt ttgccagcaa tgtcactgaa ttattaggac gtgcagctca cggaaaaggt
    15241 gtttacttag caaacgagga agatggcttg aaagtagatc tttactgtta tctagaatat
    15301 ggtgtttcag ttcctaaagt agcaatggaa atgcaggaac gggtcagcca gcaagtcctt
    15361 ttcatgacgg atatcgattt atctgaagtc aatatccatg tagtagctgt tgttcctgaa
    15421 aaattagaac aaccaacaat cgaagaactt tttgaagacg aagaggaaga gaatgaataa
    15481 agaattatca agacacgaga ttcgggaaat ggcattgcag gctttatttc cattagactt
    15541 caatgcagac ctaacaaaag aagatgcgat tttcaacgca atcgaattag accaccgcga
    15601 catgatcaac gaagatgaat cagagtttgt tcctgtttat cttgatacgc tagttggcgg
    15661 tgtctgtgca aaaaaagatg aattggataa agtgattgaa aaacatttaa aaaacaattg
    15721 gcacatcaat cggatctcaa aaatggattt ggtgatcttg cgtatcgcga tttttgaaat
    15781 gacttatgtg acagatgtac cagcggctgt tgctttaaat gaagcaatcg agttggcaaa
    15841 aacatttagt gatgaccgtt cacgtaaatt cgtcaacggt gttttatcca atgtattgaa
    15901 ag
//
LOCUS       DAYYAJ010000066        15860 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000066 DAYYAJ010000000
VERSION     DAYYAJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.066"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACHATZ_001741"
     CDS             complement(12..482)
                     /locus_tag="ACHATZ_001741"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8404265.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACHATZ_001742"
     CDS             complement(641..1099)
                     /locus_tag="ACHATZ_001742"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8404266.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACHATZ_001743"
     CDS             complement(1270..2415)
                     /locus_tag="ACHATZ_001743"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8404267.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACHATZ_001744"
     CDS             complement(2428..3345)
                     /locus_tag="ACHATZ_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8404268.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACHATZ_001745"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACHATZ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8404269.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="ACHATZ_001746"
     CDS             4437..4607
                     /locus_tag="ACHATZ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404270.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="ACHATZ_001747"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="ACHATZ_001747"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8404271.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="ACHATZ_001748"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="ACHATZ_001748"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8404272.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /gene="dapA"
                     /locus_tag="ACHATZ_001749"
     CDS             6871..7752
                     /gene="dapA"
                     /locus_tag="ACHATZ_001749"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8404273.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /gene="dapB"
                     /locus_tag="ACHATZ_001750"
     CDS             7749..8465
                     /gene="dapB"
                     /locus_tag="ACHATZ_001750"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8404274.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="ACHATZ_001751"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="ACHATZ_001751"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8404275.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="ACHATZ_001752"
     CDS             9182..10183
                     /locus_tag="ACHATZ_001752"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8404276.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="ACHATZ_001753"
     CDS             10180..11541
                     /locus_tag="ACHATZ_001753"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8404277.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="ACHATZ_001754"
     CDS             11501..12652
                     /locus_tag="ACHATZ_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8404278.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="ACHATZ_001755"
     CDS             12627..13796
                     /locus_tag="ACHATZ_001755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8404279.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="ACHATZ_001756"
     CDS             13971..15350
                     /locus_tag="ACHATZ_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8404280.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="ACHATZ_001757"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="ACHATZ_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5185 a         2799 c         3378 g         4498 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DAYYAJ010000067        15454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000067 DAYYAJ010000000
VERSION     DAYYAJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.067"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..554
                     /locus_tag="ACHATZ_001758"
     CDS             72..554
                     /locus_tag="ACHATZ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404281.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            751..1641
                     /locus_tag="ACHATZ_001759"
     CDS             751..1641
                     /locus_tag="ACHATZ_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8404282.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1967..4441
                     /locus_tag="ACHATZ_001760"
     CDS             1967..4441
                     /locus_tag="ACHATZ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8404283.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4544..6280)
                     /locus_tag="ACHATZ_001761"
     CDS             complement(4544..6280)
                     /locus_tag="ACHATZ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8404284.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6277..6981)
                     /locus_tag="ACHATZ_001762"
     CDS             complement(6277..6981)
                     /locus_tag="ACHATZ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8404285.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7112..7615
                     /locus_tag="ACHATZ_001763"
     CDS             7112..7615
                     /locus_tag="ACHATZ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8404286.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7575..7913
                     /locus_tag="ACHATZ_001764"
     CDS             7575..7913
                     /locus_tag="ACHATZ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8404287.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7934..9352
                     /gene="ffh"
                     /locus_tag="ACHATZ_001765"
     CDS             7934..9352
                     /gene="ffh"
                     /locus_tag="ACHATZ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8404288.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9464..10042
                     /locus_tag="ACHATZ_001766"
     CDS             9464..10042
                     /locus_tag="ACHATZ_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8404289.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10046..10567
                     /locus_tag="ACHATZ_001767"
     CDS             10046..10567
                     /locus_tag="ACHATZ_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8404290.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10646..11200)
                     /locus_tag="ACHATZ_001768"
     CDS             complement(10646..11200)
                     /locus_tag="ACHATZ_001768"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8404291.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11453..11728
                     /gene="rpsP"
                     /locus_tag="ACHATZ_001769"
     CDS             11453..11728
                     /gene="rpsP"
                     /locus_tag="ACHATZ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8404292.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11740..11991
                     /locus_tag="ACHATZ_001770"
     CDS             11740..11991
                     /locus_tag="ACHATZ_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8404293.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12115..12906
                     /locus_tag="ACHATZ_001771"
     CDS             12115..12906
                     /locus_tag="ACHATZ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404294.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13076..13597
                     /gene="rimM"
                     /locus_tag="ACHATZ_001772"
     CDS             13076..13597
                     /gene="rimM"
                     /locus_tag="ACHATZ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8404295.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13587..14348
                     /gene="trmD"
                     /locus_tag="ACHATZ_001773"
     CDS             13587..14348
                     /gene="trmD"
                     /locus_tag="ACHATZ_001773"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8404296.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14484..14831
                     /gene="rplS"
                     /locus_tag="ACHATZ_001774"
     CDS             14484..14831
                     /gene="rplS"
                     /locus_tag="ACHATZ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8404297.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15141..15383
                     /locus_tag="ACHATZ_001775"
     CDS             15141..15383
                     /locus_tag="ACHATZ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8404298.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5139 a         2784 c         3194 g         4337 t
ORIGIN      
        1 tttcccccta atttttttag atagaaaatt gtatagtatg atgagagaaa aagtagtttg
       61 ggggaacgtt aatgagaaga aaagcagtaa tcgatcggct ttatttattt ttttggtttt
      121 ttcctatcgt tttgctacct tttacatggg taaatgctgt tgtacaaggt gcggagatat
      181 taggcctgat ccaaggaagc ggattagtgg tttctaggaa ttttccagct ttgactgttt
      241 tctatttagt gataagtgta ggtctcttat taccaaaatc tctgatcaat cactacaatt
      301 ggcagctgct ttacttttct tgcttattag tatttacatc tatgttttct ttgctgcctc
      361 gcttgattat cggccctaat ggtttgagcg agagtggatt gaaggattca gcttattcct
      421 ccatgtattt cttgacgatc aaaccatggt tctatttagt aattgcctca tttattcttg
      481 caacattttt ttacttctgg acagaaatca ggataaaaag tgaacgatct gtatctataa
      541 aaaataacta ttgatggtgg atcatcagta aaaaagcata tgagctgcgt aaaatgcatg
      601 tttctcatat gcttttttta ttattatgta aagaatgcga aaaacgttta catgattttt
      661 acataactta actatatttt tacagcaacc atacgaggga tttacagtcg actgataaac
      721 tttagttgta aataagaaag gagcaataaa atgaaaaaag tattgtttac tctaggtatg
      781 atgggaattc ttttagccgg atgcggctca ggatcaggaa atacagatgg ctctgccaca
      841 caaaataatt caagtgactc aaatgaacaa gtaaaaatcg ttgcggtagg ttcgacagca
      901 ttacagccgt tagtcgatgc agcacaagaa agctttgtac aagaaaatcc aaattatcaa
      961 atctctgtac aaggaggagg aagcggaact ggtttgagcc aagtcgaagc tggagcagtt
     1021 actatcggaa actctgatgt atttgcagaa gaaagagacg gagttgatgc ttcaaaatta
     1081 gtggatcata aagtagcagt ggtcgggatg gcaccaatcg tcaataaaga tacagatgtc
     1141 aaagatatca ccaaacagga attgattgat atctttacag gaaaaatcac taactggaag
     1201 gaagttggcg ggaaagatca aaaaatcaac gtcgtgaacc gtgcaaacgg cagcggtacc
     1261 cgcgcgacat tcgaaaaatg gggactggat ggtgctaccc ctgtgcagtc ccaagaacaa
     1321 gattcatcag gaacagttcg gcagctggta agtcaaacac caggagcaat cagctattta
     1381 gctttctcat atctcgatga ttccacacaa gcactaagta tcgacggtgt agaaccaaaa
     1441 gaagagaatg tagcagataa cagctgggga atctggtcat atgaacatat gtacacgaac
     1501 ggaaaacctt cccctgaagt ccaaaaattc ttagactaca tgatgacaga agaaatccaa
     1561 gaaggacctg tcaaagaatt gggctattta ccaatcacaa tgatggaagt agaacgtgat
     1621 cacgaaggaa atatcaaata actagcagat aaacatgcca cggtagtcgt tactattaag
     1681 gaaaaatcaa ctatgtttcc ccacatatac atttttcctt taattgtgaa aaatagttcc
     1741 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttattc
     1801 gaaaaaaaga gctacgacat agaactgtcg tagctttttt tttgctatat gacaggaatg
     1861 aaaggtgttt gcaagcttaa tgtgaggaac actttccata tctgttctat actggatagg
     1921 agaagtttgg aaatatccag aaaacgaaat caatcgaggt agccacatga agaataaatg
     1981 gataaatcga agtaaaaaaa agaaagtaac acaaagatca tcagataaag tcatccgtct
     2041 ttttccatcg tataaagaag gattaacggc agatcaagtc aaggaacgaa tagaaaaagg
     2101 tgcagcaaat aattcagtcg atcccacatt caaaacaaat cagcaaatcg tattggaaaa
     2161 catatttacg tatttcaatt tgatctttct tatcttagcc attttgcttt gtttagtaga
     2221 atcctataag aacttgactt ttttaccagt tatcatagcc aatacaggaa tcgccatttt
     2281 tcaggaaatt cgatccaaaa agatcttgga cgaactaaat gttttaaata cagcaaaagt
     2341 caatgtcata cgagacggga tagaacaaac aatagatata gaagaccttg taatagatga
     2401 cattgttatt ttagagactg gacatcaaat tccagcagat gcagaggtcg tggaagggaa
     2461 actgcaagtt aatgaggctt tattgacagg cgaagcagat gaaataacca aagaaatagg
     2521 cgatccatta atgtccggca gtttcattgt ttccggaaaa gcttacgccc gattagacaa
     2581 agttggagca gattcgtata tttcccagct gacagtcaaa gccaagacaa tgggagaagg
     2641 agaacagtcg gagatgattg cttctcttaa tcaattgatc aaatggattg ggatcatcat
     2701 tattcctatc ggtatcctgc ttttttctca aagttacttt tttaatggaa acacgatcaa
     2761 agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg atacctgaag gactgtactt
     2821 gctgacgacg attgcattag cattaagtgc gactaaacta gcaaagcaaa gagttctttt
     2881 acacaatatg aaaagcattg agacattggc aagggtaaat gtcctttgtg tagataaaac
     2941 aggaacgatc acagaaaata aaatgtctgt tcaaaaagtg atcgtctcta aaaaacaaga
     3001 aataatcagt cagtcagatc aattggaagc atgcattgct gactatgcaa aagcgatggc
     3061 aggagacaat gcaacaatgg aagctgttaa agcatatttt acaaaaacca ctgatcaatc
     3121 ctatcaccat gttattcctt tttcttctgt ccaaaaattc agcagtgtag cgttggcaga
     3181 caaggtgtat gttttaggag cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc
     3241 ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga
     3301 tgggattctt gaagaaccag tattgacaga agcagtcaca ccattaggct atattctgat
     3361 tgctaatcct atcagaaaag aagcaagagc cacatttgat tacttcaaaa aacagcaagt
     3421 agccatcaaa gtgatttctg gagataatcc gctcaccgta tccaatgtag ctgtccaagc
     3481 agggatttca catgctgatc agtacgttga tgcatctaaa ttatcagaag aagaatatga
     3541 agcagctgtc gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt
     3601 cgttcaattg ctaaagcgtc gtaacaatac agtagctatg acaggtgatg gcgtgaatga
     3661 tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa atgaagccgc
     3721 catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc cagaagttgt
     3781 tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt ttttagtgaa
     3841 gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga cctatccttt
     3901 agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat ctttcttgct
     3961 tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg tgatggaaaa
     4021 agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga tctgcggggt
     4081 gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt tgattgcagt
     4141 agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa gtcagatcat
     4201 tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc tgttttcaat
     4261 caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct cggcagattc
     4321 gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa atgaaaaaga
     4381 gcaaaagccg tttagtcgcg tagttaaaaa atttctatcg atatttaaac gagaaaattg
     4441 acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct ttcgaagcag
     4501 gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt ttcggaatgg
     4561 taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta ccatgaagga
     4621 gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa tgccggcttc
     4681 gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca atgtcgatac
     4741 ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag cggataataa
     4801 tctcgctgtc gctttttgaa tattggacag cattttcaat cagatttttg ataattggat
     4861 aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga atcgtcaacc
     4921 gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa ggttctgtat
     4981 aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg atttcccgaa
     5041 tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa gtcagagttt
     5101 gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt gaagtcacag
     5161 gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt tctaaacgtc
     5221 gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct gaattagtaa
     5281 agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc tcctgatgga
     5341 tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc tcttgaatga
     5401 cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc tgcatctttg
     5461 gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca atcatcagtt
     5521 cgttcagtaa cgtatataat tgctcttctg tggtagtata cgcccgatac atcttactca
     5581 tttgttcgct caacgcattg atcgtctgat acaattcttc ccattgatcg ggtgtttgca
     5641 tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc agcacggttt
     5701 tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga acaaggaaga
     5761 aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt aaaaaaccgc
     5821 ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg actggtaatg
     5881 cgacatataa aagatcccca ttcaaagtag tacttttgcg caaagcgcta cctaggttcc
     5941 caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc aatgtttcat
     6001 tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt tcgttcgact
     6061 gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt tcgacatcta
     6121 attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt tccacgatct
     6181 gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta actaaaagaa
     6241 taagaaagaa gagacgttca taaatttttt tccatttcat cgtttgggtt cctgaaactt
     6301 atagccaaat cctctaatag tcaccaagta ctttgggtgt tttggatctc gctcgatttt
     6361 ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga agtcaaaatt
     6421 ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt tcatgaaata
     6481 aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta cggtcacttg
     6541 ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct cagcttcatc
     6601 ttttgattgc ttacgaggtt caattcgtct gaatattgct ttcatacggg ctaatacttc
     6661 gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc caatgatccg
     6721 atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt tttcttgacg
     6781 cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta aaatgatgaa
     6841 atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg cactggttac
     6901 cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag gttcatcatc
     6961 aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct gtttatattt
     7021 tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat aataggaagg
     7081 cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat gtttaggcgg
     7141 tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg aactagttga
     7201 aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa ttcctagaga
     7261 tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag caaaagtatg
     7321 gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca aagcctatca
     7381 aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc cttcatgcgg
     7441 aacccattcg atttatgacg ggagtttttc cggaataaaa aaaaatggtg ccggagtggc
     7501 tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat ggctgaaaac
     7561 tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc tttgaatttt
     7621 attcgacctt attgacagaa aaacaaatga actacatgga attatattat gcagatgatt
     7681 tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta tatgacaata
     7741 tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa acttcatctt ttttctaatt
     7801 atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat aagtacccgg
     7861 aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa taaataaaag
     7921 gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg cgatgggaaa
     7981 aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc gggaaatccg
     8041 ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta ctaagcgcgt
     8101 acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac aacaaatcgt
     8161 aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag agttaaataa
     8221 atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag gtaaaacgac
     8281 ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc cgctcctaat
     8341 cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag gtcaacagct
     8401 agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg tccgtcaagg
     8461 gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg caggacgtct
     8521 gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga cgcaaccaaa
     8581 tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg tagctgatag
     8641 cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg gggatactcg
     8701 aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat tcatcggttc
     8761 tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta gccgtatcct
     8821 tggtatgggg gatatgctga cattgattga aaaagcgcag caagactatg acgagaaaaa
     8881 agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg actttattga
     8941 acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa tgatcccagg
     9001 aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg ttgaacgcaa
     9061 aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc tgttgaaccc
     9121 aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag tgaatcgaat
     9181 gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag gggatatgaa
     9241 catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag gcaaaatggc
     9301 gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat aagatgaaaa
     9361 aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc agcaattttt
     9421 attctttact aatacaatta ctctaaacga agaggtggca cagatgagat gtccgtggag
     9481 tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc ctgaatatga
     9541 tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat tgagctggca
     9601 gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg atattaaaaa
     9661 agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag gaattattcg
     9721 taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga aaattcaaga
     9781 agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa tcgttgattc
     9841 ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg aattagcaaa
     9901 agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg cttttttaga
     9961 agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag aggtcgtacg
    10021 tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg ctataagttt
    10081 agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca ctccaacagg
    10141 taagcaaacc acttatgaag ctttttatga aacaatcgat acgaccatca tgggcagaaa
    10201 aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag agaaaaagaa
    10261 tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg taaacgaaga
    10321 tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct gggttgtggg
    10381 tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat ggtatatcca
    10441 gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa catatgaagc
    10501 gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc attatgtacg
    10561 aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct gtacatagtt
    10621 gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa tctagatacg
    10681 catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca ttttcatcaa
    10741 ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc acattcaaaa
    10801 agacaagtga ttgtcgtaga tggtggtttg cgccaaagcc gccgatccca cctggtgatg
    10861 cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt cttgagccta
    10921 catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga gtaacgaaaa
    10981 cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca ggagcctgtt
    11041 ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca aatacacttt
    11101 cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg taagaatctt
    11161 ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc ctccttcaaa
    11221 ctatctataa caatcttaca aaaaataagt aactggagca aataaaaaca cttactgaca
    11281 aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa aatcttctat
    11341 ttcctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac agggtaaaaa
    11401 aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa taatggcagt
    11461 taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta tcgtcgtagc
    11521 tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt acaatccttt
    11581 gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt tatcaaaagg
    11641 tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta tgcaaaaaca
    11701 ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt aagcgactta
    11761 gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt agacgtcgta
    11821 gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat tggccgtatc
    11881 attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg tgttcggatc
    11941 aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta aataaacgtg
    12001 ctgtactcga attttcgagt gcagcttttt tgttttttta ttaattgaag aaatgattat
    12061 ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga
    12121 aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa
    12181 gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa
    12241 agtagtacca aaacggctac caccactgaa caaacaatgg ctacctcaaa taatgagaaa
    12301 aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta
    12361 ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt
    12421 ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg
    12481 cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt
    12541 ttgcagccct ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca
    12601 gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt
    12661 attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc
    12721 gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt
    12781 gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc
    12841 tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg
    12901 ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc
    12961 aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa
    13021 ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac
    13081 tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt
    13141 tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgt taacactatt
    13201 ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt
    13261 tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg
    13321 catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca
    13381 tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat
    13441 cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt
    13501 gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt
    13561 agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa
    13621 tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag
    13681 atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt
    13741 atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc
    13801 aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg
    13861 gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca
    13921 tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt
    13981 ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa
    14041 cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt
    14101 caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag
    14161 taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat
    14221 cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc
    14281 aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag
    14341 ccaagtgact ttttctaaaa tattctttac agtaggttga taatatgtta gtattttatg
    14401 gtgagtgcaa aagcacttaa ctattacgat attccgctat gagaaaagct cataagaata
    14461 tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat
    14521 tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg
    14581 ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg
    14641 ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta
    14701 cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac
    14761 gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa
    14821 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
    14881 ttcttagcat gtaatttgaa caccctgatc cagttttttt ctttaattat tggtagggga
    14941 gaataatgga ttagaataaa tctacctata agtagtagta aaaaaaagag cactatgata
    15001 atatctttct taagagtaac cgctttaata aaaaatccag aatattctaa cataaatgga
    15061 gtattcttag aatcaaaagg agaaagaaga tggtttatac ttatacggag caagaaaaaa
    15121 tgaaagtttg agacacattc atgaatgatg gaaaagtaaa acaagtacca agttcaacaa
    15181 aaaagaaaaa catcctattg aaagaagtct tgaaacgctt tgaccatgga gtgacttata
    15241 cagaaacaga ggttaattcg atactgctga acgtattttc tagtggcgat tatgtcgaac
    15301 aaagacgtta tctaatcaca tttggctttt tcaaaagaag ttcagacggt cgggcatatc
    15361 agatgatggg aattgaaaat tagaagtttt aatttatact cattgaaaaa agttcaagta
    15421 tgcgatattg gttctacacc gcatacttga actt
//
LOCUS       DAYYAJ010000068        14820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000068 DAYYAJ010000000
VERSION     DAYYAJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.068"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHATZ_001776"
     CDS             complement(296..1024)
                     /locus_tag="ACHATZ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404299.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHATZ_001777"
     CDS             complement(1002..2576)
                     /locus_tag="ACHATZ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8404300.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHATZ_001778"
     CDS             2805..3743
                     /locus_tag="ACHATZ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404301.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHATZ_001779"
     CDS             3755..4666
                     /locus_tag="ACHATZ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404302.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHATZ_001780"
     CDS             4690..6162
                     /locus_tag="ACHATZ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8404303.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHATZ_001781"
     CDS             6213..6824
                     /locus_tag="ACHATZ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8404304.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHATZ_001782"
     CDS             6854..8767
                     /locus_tag="ACHATZ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8404305.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHATZ_001783"
     CDS             8895..11876
                     /locus_tag="ACHATZ_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8404306.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHATZ_001784"
     CDS             11873..13534
                     /locus_tag="ACHATZ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8404307.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHATZ_001785"
     CDS             13574..14506
                     /locus_tag="ACHATZ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8404308.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2333 c         2979 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DAYYAJ010000069        14530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000069 DAYYAJ010000000
VERSION     DAYYAJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.069"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..247)
                     /locus_tag="ACHATZ_001786"
                     /pseudo
     CDS             complement(101..247)
                     /locus_tag="ACHATZ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            486..1178
                     /locus_tag="ACHATZ_001787"
     CDS             486..1178
                     /locus_tag="ACHATZ_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8404309.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(1460..1532)
                     /locus_tag="ACHATZ_001788"
     tRNA            complement(1460..1532)
                     /locus_tag="ACHATZ_001788"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Lys,seq:ctt)
     gene            1913..2482
                     /locus_tag="ACHATZ_001789"
     CDS             1913..2482
                     /locus_tag="ACHATZ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8404310.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2469..3329
                     /locus_tag="ACHATZ_001790"
     CDS             2469..3329
                     /locus_tag="ACHATZ_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8404311.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3519..4223
                     /gene="yycF"
                     /locus_tag="ACHATZ_001791"
     CDS             3519..4223
                     /gene="yycF"
                     /locus_tag="ACHATZ_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8404312.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4228..6063
                     /gene="walK"
                     /locus_tag="ACHATZ_001792"
     CDS             4228..6063
                     /gene="walK"
                     /locus_tag="ACHATZ_001792"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8404313.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6060..7376
                     /gene="yycH"
                     /locus_tag="ACHATZ_001793"
     CDS             6060..7376
                     /gene="yycH"
                     /locus_tag="ACHATZ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8404314.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7377..8246
                     /locus_tag="ACHATZ_001794"
     CDS             7377..8246
                     /locus_tag="ACHATZ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8404315.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8301..9110
                     /locus_tag="ACHATZ_001795"
     CDS             8301..9110
                     /locus_tag="ACHATZ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8404316.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9213..9842)
                     /locus_tag="ACHATZ_001796"
     CDS             complement(9213..9842)
                     /locus_tag="ACHATZ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8404317.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10012..11304
                     /locus_tag="ACHATZ_001797"
     CDS             10012..11304
                     /locus_tag="ACHATZ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8404318.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11380..12042)
                     /locus_tag="ACHATZ_001798"
     CDS             complement(11380..12042)
                     /locus_tag="ACHATZ_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8404319.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12267..12551
                     /gene="groES"
                     /locus_tag="ACHATZ_001799"
     CDS             12267..12551
                     /gene="groES"
                     /locus_tag="ACHATZ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8404320.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12602..14227
                     /gene="groL"
                     /locus_tag="ACHATZ_001800"
     CDS             12602..14227
                     /gene="groL"
                     /locus_tag="ACHATZ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8404321.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4915 a         2456 c         3071 g         4088 t
ORIGIN      
        1 ccattattat ctagtctagt ccattctttt tgtttgcagt atttgtgatg gctttccgta
       61 cccttagctc agttggtcag agcagacggc tcataaccgt ccggtcgtac ttctcgtaat
      121 ccaggaaggc cgtcagctcc gtccgcagta gcgtgtttac ggccagctcc agatgggagc
      181 ggaataactc gttcaaatca cctttggtgg ctagagtctg caggagttct gtaataaaat
      241 cgttcatagg gaagtcctct tttctgtgaa tgggtgtggt aactttattc tacagaaggg
      301 acttcctttt ttctatttac acaaaatatt ttatactctc aaatgtttgt atgcagttac
      361 atctgacttt attcgaatta aggaaaagat ttataaaaat aaaataaaaa aatatatatt
      421 taaattgact tattgtgaaa tcattaacta taattagttc agtttatgtt aaggagaatg
      481 atgatatgaa taagaaaatt gtagtaagtg gattaattat aggtggatta gtgttagcag
      541 ggatcgcagg gaaaaacttt gccgatgaca ttttgttatg gggtggagaa gataatattg
      601 aacaaatcaa tcataatttg gagtcattag ataaagcact agaaaataaa gagcaaaata
      661 ttagtagttt aaattcacga ttatcttcaa ataatcaaga acttgagcaa gcaaaggcta
      721 acgttgaaga atataaacaa aaagtgttga ctttggaaaa tgataaaaat caattaacta
      781 tagagaaaac aaatttagag aatcaattat caggaaaaaa tgctgaattg caaggtaaac
      841 aaaatgaaat caatgctaaa attgatgaaa taaatcaaaa aatagctgaa ataaatcaat
      901 taaacactga ttggaataat cgtttacaaa cagaacaaaa taaatttaat gatgctcaaa
      961 atactgttaa tagtttaaat caacaaatta atcaattgtc aaatgaaaaa aatactatta
     1021 aaaaccaatt gcaaagtact gaacaaaaac tagaaaaagc gcaatcagaa aacagtaatt
     1081 tgaataatta tattgaaaaa ctggaaaaag ccaaaagcga tgtagaagac acagcaaaca
     1141 gatcacaaga aatcgtgact gaacatacag gaaaatagat gcatttttta gtttaaattt
     1201 ctttaacaaa atagtatttt gttgctaaat aatttttatt tgaatattct acaatgatat
     1261 ctcccctcaa aatggagggg aagtacatac tgtgataatc tacaggttta ttgactgatt
     1321 aggatgttat taaaaaataa aatactaaaa tgcatacccg aatttcatac ccgaaaaata
     1381 aaaaatgcca ccacgtacac tcttttcgaa actacctaag tagccaagaa tgtactggtg
     1441 acgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
     1501 gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca
     1561 ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt
     1621 ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa
     1681 caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc
     1741 gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt
     1801 tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg
     1861 tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa
     1921 aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg
     1981 ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta
     2041 tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa
     2101 ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt
     2161 agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga
     2221 agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga
     2281 tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca
     2341 aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt
     2401 tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga
     2461 gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag
     2521 aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc
     2581 aggtatatac agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg
     2641 aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca
     2701 aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggattg agcgggacat
     2761 ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata
     2821 cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag
     2881 aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag
     2941 ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg
     3001 cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga
     3061 tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt
     3121 acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg
     3181 cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc
     3241 aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg
     3301 gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc
     3361 agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat
     3421 tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat
     3481 gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg
     3541 atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg
     3601 tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt
     3661 tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac
     3721 gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag
     3781 tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat
     3841 tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag
     3901 aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg
     3961 tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact
     4021 tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt
     4081 atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga
     4141 tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt
     4201 tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc
     4261 aattttaaga tcgctattac ctttattctg attttattga tctcgatcga aattattggt
     4321 gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat
     4381 caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca
     4441 gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat
     4501 atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat
     4561 gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa
     4621 cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg
     4681 tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa
     4741 tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt
     4801 atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg
     4861 agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga
     4921 caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa
     4981 gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca
     5041 gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt
     5101 tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat
     5161 atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac
     5221 cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc
     5281 cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa
     5341 gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca
     5401 ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag
     5461 gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg
     5521 atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa
     5581 tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga
     5641 aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa
     5701 gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat
     5761 tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta
     5821 agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg
     5881 ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct
     5941 atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga
     6001 aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa
     6061 tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat
     6121 acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga
     6181 caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc
     6241 ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt
     6301 accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag
     6361 gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata
     6421 atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga
     6481 acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata
     6541 aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga
     6601 attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa
     6661 cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt
     6721 atagttatat cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac
     6781 cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa
     6841 caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa
     6901 aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt
     6961 tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg
     7021 aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg
     7081 aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga
     7141 taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag
     7201 ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg
     7261 tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata
     7321 actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg
     7381 atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat
     7441 tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg
     7501 aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg
     7561 agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga
     7621 attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg
     7681 ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa
     7741 ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt
     7801 atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc
     7861 aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa
     7921 aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc
     7981 agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca
     8041 ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc
     8101 tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg
     8161 ataaagtggt ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata
     8221 ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta
     8281 agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag
     8341 caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg
     8401 atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg
     8461 accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg
     8521 tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg
     8581 aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca
     8641 gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc
     8701 aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt
     8761 gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc
     8821 atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag
     8881 gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta
     8941 atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg
     9001 ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg
     9061 aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat
     9121 caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt
     9181 tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc
     9241 ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg
     9301 atcgtactgt cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct
     9361 gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt
     9421 ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt
     9481 gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa
     9541 gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg
     9601 gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca
     9661 acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga
     9721 acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac
     9781 aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc
     9841 ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag
     9901 aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata
     9961 gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag
    10021 tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact
    10081 gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc
    10141 ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct
    10201 atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt
    10261 gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca
    10321 gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg
    10381 acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat
    10441 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
    10501 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
    10561 tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc
    10621 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
    10681 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
    10741 cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg
    10801 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
    10861 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
    10921 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
    10981 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
    11041 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
    11101 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
    11161 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
    11221 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
    11281 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
    11341 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    11401 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    11461 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    11521 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    11581 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    11641 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    11701 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    11761 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    11821 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    11881 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    11941 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    12001 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    12061 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    12121 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    12181 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    12241 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    12301 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    12361 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    12421 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    12481 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    12541 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    12601 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    12661 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    12721 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    12781 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    12841 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    12901 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    12961 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    13021 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    13081 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    13141 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    13201 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    13261 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    13321 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    13381 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    13441 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    13501 tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa
    13561 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    13621 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    13681 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    13741 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    13801 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    13861 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    13921 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    13981 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    14041 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    14101 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    14161 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    14221 gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa
    14281 gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt
    14341 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat
    14401 acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc ttttttgtgt
    14461 gtaattataa tgaaaatagt tatttttaat agttataata ttttatctat taaaagtaaa
    14521 tatccattca
//
LOCUS       DAYYAJ010000070        14465 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000070 DAYYAJ010000000
VERSION     DAYYAJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.070"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHATZ_001801"
     CDS             complement(227..1114)
                     /locus_tag="ACHATZ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404322.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHATZ_001802"
     CDS             complement(1111..1980)
                     /locus_tag="ACHATZ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404323.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHATZ_001803"
     CDS             complement(2117..3403)
                     /locus_tag="ACHATZ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8404324.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHATZ_001804"
     CDS             3676..4254
                     /locus_tag="ACHATZ_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8404325.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHATZ_001805"
     CDS             4254..5183
                     /locus_tag="ACHATZ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404326.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHATZ_001806"
     CDS             5180..6916
                     /locus_tag="ACHATZ_001806"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8404327.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHATZ_001807"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHATZ_001807"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8404328.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHATZ_001808"
     CDS             complement(7812..8321)
                     /locus_tag="ACHATZ_001808"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8404329.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHATZ_001809"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHATZ_001809"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8404330.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHATZ_001810"
     CDS             complement(9622..10713)
                     /locus_tag="ACHATZ_001810"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8404331.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHATZ_001811"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHATZ_001811"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8404332.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHATZ_001812"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHATZ_001812"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8404333.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHATZ_001813"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHATZ_001813"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8404334.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4123 a         3147 c         2614 g         4581 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acata
//
LOCUS       DAYYAJ010000071        14377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000071 DAYYAJ010000000
VERSION     DAYYAJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.071"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(553..1737)
                     /locus_tag="ACHATZ_001814"
     CDS             complement(553..1737)
                     /locus_tag="ACHATZ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8404335.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1762..2769)
                     /locus_tag="ACHATZ_001815"
     CDS             complement(1762..2769)
                     /locus_tag="ACHATZ_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8404336.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2941..3300)
                     /gene="comGG"
                     /locus_tag="ACHATZ_001816"
     CDS             complement(2941..3300)
                     /gene="comGG"
                     /locus_tag="ACHATZ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8404337.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3272..3697)
                     /gene="comGF"
                     /locus_tag="ACHATZ_001817"
     CDS             complement(3272..3697)
                     /gene="comGF"
                     /locus_tag="ACHATZ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8404338.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3660..4007)
                     /locus_tag="ACHATZ_001818"
     CDS             complement(3660..4007)
                     /locus_tag="ACHATZ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404339.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3979..4449)
                     /gene="comGD"
                     /locus_tag="ACHATZ_001819"
     CDS             complement(3979..4449)
                     /gene="comGD"
                     /locus_tag="ACHATZ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8404340.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4424..4702)
                     /gene="comGC"
                     /locus_tag="ACHATZ_001820"
     CDS             complement(4424..4702)
                     /gene="comGC"
                     /locus_tag="ACHATZ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8404341.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4699..5748)
                     /gene="comGB"
                     /locus_tag="ACHATZ_001821"
     CDS             complement(4699..5748)
                     /gene="comGB"
                     /locus_tag="ACHATZ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8404342.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5705..6634)
                     /gene="comGA"
                     /locus_tag="ACHATZ_001822"
     CDS             complement(5705..6634)
                     /gene="comGA"
                     /locus_tag="ACHATZ_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8404343.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6766..8520
                     /gene="ade"
                     /locus_tag="ACHATZ_001823"
     CDS             6766..8520
                     /gene="ade"
                     /locus_tag="ACHATZ_001823"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8404344.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8618..9280)
                     /gene="trmB"
                     /locus_tag="ACHATZ_001824"
     CDS             complement(8618..9280)
                     /gene="trmB"
                     /locus_tag="ACHATZ_001824"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8404345.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9350..10132)
                     /locus_tag="ACHATZ_001825"
     CDS             complement(9350..10132)
                     /locus_tag="ACHATZ_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8404346.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10336..11547)
                     /locus_tag="ACHATZ_001826"
     CDS             complement(10336..11547)
                     /locus_tag="ACHATZ_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404347.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11540..12277)
                     /locus_tag="ACHATZ_001827"
     CDS             complement(11540..12277)
                     /locus_tag="ACHATZ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404348.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12415..12849
                     /locus_tag="ACHATZ_001828"
     CDS             12415..12849
                     /locus_tag="ACHATZ_001828"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8404349.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12842..13186
                     /locus_tag="ACHATZ_001829"
     CDS             12842..13186
                     /locus_tag="ACHATZ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8404350.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13366..14376
                     /locus_tag="ACHATZ_001830"
     CDS             13366..14376
                     /locus_tag="ACHATZ_001830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8404351.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4576 a         2929 c         2422 g         4450 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaaccg
      121 ttttttcgca acaaaaaact tgattttcat acctgtgctt tcacgattca ggcaaagtac
      181 aggtggaaaa tcaagttttt attattcgat caatgaatgt ttcggatcgg ttttctagct
      241 aaacataaag cgaagaataa agtcgacagc ccacgaggct gatctaaaaa tgttttttct
      301 ttttacatat gtaatgtccc ttataaagat gaatgaaaat aaaatattgg gattttcctg
      361 aaaatcaata aataaaatgc cagtcttttc ccttttgacc acttttccaa gcaaaattcc
      421 ctaagaagat cctgtaaaaa agaagggaaa ttgaagttaa attaccattt tgcggtcttc
      481 tccgcaaaaa aaacacatgc aagaattccc tcttgcatgc gtttcatctt ttatcctaac
      541 gaatgctttg gcttagccgc gcagacgctc cacatcacgg gcaatcatta gttcctcatc
      601 tgttgggatc actaatactc ttacttttga ttcttttgta gagatatcta cttcttcacc
      661 acgcaattca ttcaacgctg gatcgatttc acaaccaaac caagacatac ctttgatgat
      721 ttcactacgt acatgtccgt cattctcgcc gataccagca gtaaagacaa ttgcatcaac
      781 accattcatc gttgtcacat aaccgccgat gtattttcgg atgcggtcca cgaaaatgtc
      841 ataagctaaa cgaatatgtt ctttttcata gttcttttca agatcacgca tatcactaga
      901 caatcctgtt aggcctagca gaccagattt cttgttcaaa acattgatca tttcatcaat
      961 atcaatctct aatttttcca ttaaatatgg gagaacagct ggatcgatat cacctgaacg
     1021 tgtacccatt gtcaccccag ctaatggtgt gaatcccata gaagtatcca tggatttacc
     1081 tccatcgact gctgtgatcg atacaccatt tcctaagtgg caagtgatga tttttagttc
     1141 ttcgatcggt ttgttcaata attttgccgc acgttccgac acataacgat gacttgtgcc
     1201 atggaagcca tattttctta ctttgaaatc ttcataataa gacaccggta agctatataa
     1261 ataattttgc tttggcattg ttgagtggaa agatgtatcg aatacagcca cactcaaaat
     1321 gtcaggtaaa atctttttaa acgctttgat ccccattaag tttgcaggat tatgcaaagg
     1381 agcaaattct gctaattcag caatgcggtc cattactttc tcatcgatca ctactgaatc
     1441 cccataagtt tctcctccgt gaacaacacg gtgccctact ccggtgattt catcataaga
     1501 gcttaaaatt tgcagttcaa tcaactgatc taacagcatt tttactgcta cttcgtggtt
     1561 ttcgatatcc atgatttgtt cgaatttttg attatcgccg tacttgatgg taaaaataga
     1621 atcttgtaaa ccgatacgtt cgacgatccc tttcgcaact acggtttctt ctggcatttg
     1681 atataattgc catttcaaac ttgaactccc agcattgatt gcaattgttt tagacatatg
     1741 actctccttc tcgttgaatg attacagagc agatgatttc cagtttttaa actcgttgaa
     1801 aaattccgtc accttctctg gttcttttaa tgaagcaagt ttcacaagca agacttcttt
     1861 tacttgttgt gcttgtggtc cttttttctg taagatcaaa atactttttt gcgattgttt
     1921 attacggaac aattcatctg gcaattgaat cattccttga agataacctt cttctttaaa
     1981 ccattttttg atttcttcac tttgatccgt ttctaaaaat ccactcggca tcaaaaacaa
     2041 accaaaacca tccggcttca catatttcat tgattgctct agcaataaat gatgtgcata
     2101 actatggcct tcttcggtac ttgttaaaaa ctcttttgct ttttcgtcat tcggataata
     2161 accgattggc aaatcgctga ttgcaaaatc gacaggatct attaataatt cctgcaaccc
     2221 gtcttgatga aagtattgaa catttgcttg tgtcaaatca cttgtagaag ctgcgacagc
     2281 caacaatgta tcatcaatat ccacaccaat tccttctgtt tggtaccccg cattcgatag
     2341 gtttaaaaga actgttaata acaagttgcc cattcctgcc gcaatatcca gaatcttaac
     2401 cggtgctttt ttatttgtgt atagttgttc aataagaaaa acaaataaaa aaccgatact
     2461 atcaggagtg agttgatggt tcgcctgcaa atgttctgtc tggctgccct tcaataaaag
     2521 taattgagac agtctgcgcc attcttctgg ctcgaaagaa agtttcttca actcttcata
     2581 aagagctgtg atccgttgcg ttgtctcttt tgtcggcact ccatccacca cacgtacttg
     2641 ataatcatct attaaatttt ctgcattttc gacataggct tctaaaaagg aagttcccaa
     2701 tgcattctgt aaaagctgaa tagcttccag gttttgatta aacccttgtt ccattttttc
     2761 tggtaacatg gtgcacctca tttctagata tattataaca attttttcac ctgtttaatt
     2821 ctatcgaaca attgacaaaa tttcaatcgt tttcactgtt ttttatttgt gaaacggcaa
     2881 acattttttt attaaaaaca gtgttataac agaaaagagg gcgttttcac gaacattcca
     2941 tcattctgta ctagtatctg tatcattttt tacttcttct gtgaaccgaa agataaacgg
     3001 tgagacttcc acctctaaga atagggactg ttgtacccac tgatatgtta cagatccagt
     3061 tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta gactgttccg ataaagccat
     3121 ctgcttcatt atttttgcta tataataatt tttgattcgt atattaagat ccattgacaa
     3181 acggtagctt tctaatgctg ccatcaataa aaacgtacat aaaagaaata aaagcaagac
     3241 agaaaataaa atagagccct tataacattt tctactgatc gttttttgtc catatggcat
     3301 atccatcttc tccattttca aagtgtacat aaaaagaaat tgtgtgttct ttttccaata
     3361 attgaagctc agtgatactt gttaacatag gttgatgtcc gccggaagtt cttcttttac
     3421 gtatcattgc ctgatatctt tcgatgatat actgtttatc ctcgatcgtg tagtgcagtt
     3481 gctgattttt cacagagaca ttgtgactgc tcttgagttc ttcttctaat tgtgctaaaa
     3541 atattagcca ctctttttga tctttacgct gcaaaaattc acttacttgg atggtttgct
     3601 gaatcaaaag attgaatgtg agcaatagac tgctcaatat catcagtcca attagacatt
     3661 caatcagcgt aaatgctgga attacttgtt ttttcaatac tgatcgcttc tttctccata
     3721 ttgacaaaca ctttttcgat tactccatta ttatcgacaa tttggatatc gtatgggatc
     3781 atttcttccc ttcgctctgg tcttttgtga tacatccgct gcgtcttgat atcttcataa
     3841 atcattcgaa taaatagcag ttcctcccca cttttctttt ctgcctgaag caaaaacagc
     3901 tggccaaagc tcactaagta gacacagaca gaagcgatca taagcgctgt aagggactcc
     3961 aacaaaacaa atcctgtttt attcaattct ttttgtatag cgaccactcc ctatctggaa
     4021 ttgatacgta atcgtccgct ctttttcttc ccaataaaat tgataagctt ttagtgaact
     4081 ttcattcccg gttctagcag caaaagtaat agaagcatgg cgtttcaagg taatctcatc
     4141 aggaatatct aatacttccc aatttatttt atctggattc ggaacatgaa aaatgatttg
     4201 attctcttca ctattccaat aaaagcctgt ttggatctga ttcacaatag cgattttttg
     4261 cgtagaatag attcgttttt caaattggcc gaaaaattga tagactgcca attcattctt
     4321 ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt gtaagtaata aaatcagtaa
     4381 gagtgattca aacaacgtat atcctgatgc taaaacctta ccttcaaggc cgctgataga
     4441 tttctgcatg ttctcgtttg atatagcctt cattcaacaa ttcctccaca gtaggaactc
     4501 ttccattatt ttgaagcgca tagagctctt tttgtgtgtc taccaactgg atgatcgctt
     4561 cacgactttc ttggtctaca tgattcctat aacgcgacaa attagggacg aacaacaaca
     4621 ctaatacact gatgatcagt aagacgatta gcatttcgat caatgtaaaa ccgctataat
     4681 aagatatttt tctctttttc atggcaaaac ctcctctatg cttccgtaaa caggtaataa
     4741 aacagcggca taaactaata aaatcaaagc cgctacgccc aaaaaaatga ttggctgaat
     4801 aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt cttgtccatt cgctgtaaaa
     4861 aatcagttct ttacctaaac ttccctttgc ctctccctgt aaaacaattt tactgaattc
     4921 ttctgataga aaacgataat tctggaactg ctcagccaat gacatacctg cctccaatcc
     4981 ttgaatcaac ttagaggcaa gatgctgaat cagactttct tgtttcgttt cctttaagca
     5041 gactatgatt tgccttaatt cgaaaccttc atgaaaaagc tttccaatct ctagtgacaa
     5101 ataagccgat tgatacaagg aatatatcgg tccaatcaat aattgtctgc ttatccattc
     5161 actctgatca acaggagaca acttcatcag acgatacctt actagtaaag ccaatagagt
     5221 aaccgctagg atgaatagta gaacatacca atgaaagact tgtatcagct gaatacccca
     5281 atgctcttgc atgaccattc ctgaaccaat aagctgaggc aaaacgaaca accgcatact
     5341 aaacaagata cctagcaaaa aggtgagcaa tagcatcgga tatgcaagca gctttttcaa
     5401 atcattgcga aatccttcaa caagccgaaa ctgtttggca atttccaaca aagttgtcgg
     5461 cagattccca tgacgttcag ctaactcgat ctgtaccagt tgctctgctc catatcctat
     5521 ttgcgaaagt acctcactaa acgcttgccc ttcctttagc tgatgttgga tatttgccaa
     5581 aataacaggt ggaaacgaac ggatcgtgag taataaataa agggattctt gcagactaaa
     5641 accagataaa agcagttctc ccatggtttg tgcaaactgg ttttgctggc ttcttgttaa
     5701 tcctttagaa tagttctgac tgccaaatat tttcctgagc ttcttcataa ctttcctccc
     5761 atgatttttt tactgattct tcctccataa attgataact ccataaaaca gaaggatggt
     5821 gattggcatc ttccaaaagt tcttgataaa tgacaccagc taaacattca gatagctctt
     5881 cttttttctt tgtcagttca tggattctga ccaatgtacc ttcaagattg cgtgcatgta
     5941 cggttgcaaa aacccggtgc cctgttagtg ctgctcttac cgcagcatgt gcagttgatt
     6001 catctcttat ctcgccaatg attagcaagt ctggacgatg ccgtaatgcg agtctcaaaa
     6061 gtgtatcata agtctgtgct attttcggat tgatctgtag ctgcagaaaa aacggttctt
     6121 ctatctctac aggatcttcg atagtaatca cttgcaattt ttcttccaaa gccaaccgat
     6181 acattagaga agtcttgcct gatccgactg gtccgctgaa aaggtataat ccacgtctga
     6241 cggttttctg taaaatagtt gcaaaatctc gagaactaaa gcaacgatgg ggtgtttcaa
     6301 aagtaccgta taatagccga atcactaaac tctctcgttg aaggtaatct cctacagaag
     6361 acaaccgcag acgaacttcc tgtttttcta aagaataagt aaatgcacct aactgagctt
     6421 ttcttttttc tccgatatcc atctgtccca aatacttaaa tcttgagatc agttgctgtc
     6481 catctgccga agagactttc ttccagaatg ttcgtttttt tccatagcga aaatagatgt
     6541 aaaatacttc tgaagctggc aatatatata cgtcttgggc atgtttggca aaactttcac
     6601 ttatcatttg atccgataat cgttttattt ccattattct cccctccaca aaaaacatac
     6661 gtctttttga tctttttgtt tcaaataaaa tgaatttaca aaaaaatatt ggcaaaaacc
     6721 gaatttatgt tatacttata aaaatttatc ttgggaggca ctccattgga aacaacatta
     6781 aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg acctagtgat caagcatgct
     6841 aagatcgtca acgtctttac gaaagaaatc atggaaaaag atgtagcaat ctgcgaagga
     6901 atgatcgtag gaatcggtga atacgaagga aaaagcgtac ataatgctaa tgggcaatat
     6961 ctagtgccag gattcatcga tggccatgtg catatcgaaa gctcactttt atcaccaagc
     7021 gaattttcaa aagtatcctt gcttcatgga gttactactg tagtgacaga tccccatgaa
     7081 ataggaaaca ttgctgggag tacaggtctt gagtttatga tagaagatgc aagacaaaca
     7141 ctcatgaata tcttttttat gctaccttct tgtgtacctg taaccccttt tgagaccaat
     7201 ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac aaaaacccga agttttaggc
     7261 ttagctgaag tcatgaacta tcaagcagta gcaaataatg aaacagacat cctagaaaag
     7321 atccaattga tgcaccagca taagaaaaaa attgacgggc atgcagctgg tatcggcatg
     7381 gaagagttaa atgtttatcc tgcagcaggt attcggacgg atcatgaagc tacaactgca
     7441 aaagaagcta aagaacgttt ggatcttggt atgtatttaa tggttcgaga agggaccgtt
     7501 gcaaaagatc tggcatcgct actaccggcg atcacacctg aaaattcaag aagatgcttc
     7561 tttgtcaccg atgataaact gatcaatgat ttagtaaaag aaggatcaat cgatcatatc
     7621 gtccgtcaag ctattgcact tggaatcgat ccattacaag ccattcagat ggcaacattg
     7681 aatgcagcag aatgttttga tttgaaaaca ctaggtgcga ttgcccctgg gtatcaagct
     7741 gatttcttct taacagacga tcttaccaag ctgccaattc atgaagtttt ctataaggga
     7801 acattagttg ttcaatctgg gcaactccaa gaccatttat ttgctgatca ttcaaattca
     7861 tatacgtgtt gtctgccaaa actaaacgct caaccattaa atccacattc gttagagctg
     7921 cttctttctt cttcacaagc ccatgtgatc gaaattatcc cgaatagtct agtgactaaa
     7981 gatttggtag aaaaagtgga ccgacaaggc gaacgattca ttccttcaat cgaaaaagac
     8041 caattaaaga ttgcggtgat cgagcgtcac catcaaacgg gaaacattgg attaggcata
     8101 gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa cgattgccca tgactcgcat
     8161 aatctagttg tcgttgggac aaacgacgag gatatgctgt atgctgctaa tcttttgatt
     8221 caaaaaggtg gcgggatgac cgttgtcaac aatcagcagg aaatagcttg tgtttctctt
     8281 cctataggcg gaatcatgac acaggaacct ttttcgatcg tctatgaaca gttgaatgaa
     8341 ttaataacta aagcacatca actaggagca gctaaaacct ttgatccttt tttgacactt
     8401 tctttcttaa cattatctgt catacccgaa ttgaagatca ccgacaaagg attattttca
     8461 ttttctcgct tccaattgat tccaccttgt ggaaaaaccg atgaaataaa tacattttaa
     8521 atcaaataaa tcccagaaaa tacattacat ctgtaatgtt cattttctgg gatttattta
     8581 tggatattaa tgtttttatg aaagcagaaa gttagtttca cagacttttc tttttggtct
     8641 tatcttgaaa ttgagcttct actcgataaa tacgttgacc ttttgaagaa aatttttctt
     8701 catactccgt cataatattg ccttcaaatt gactttgatg taaatctaac catacctgtt
     8761 tgaggatcat gccatattga gaaaaacttg ccagagaata ctcaaacaat ccttgattat
     8821 ctgttttgaa atggatttct ccatttggac gaagaatctg ttcatctaca gcaagaaaag
     8881 tcttatatgt caatcgccgt ttttcatgac gtttttttgg ccatggatcg gaaaaattca
     8941 gatagatttg atctacttcg ctgtcagcga aatactctgt caacgctgat ccatccacat
     9001 gtagaagttt caggttagga agcggttgtt cgatcaactt gtctagtgca atcgaaacaa
     9061 cactgacctg catttctact ccaatataat tgatttcagg atgggctttt gccattccag
     9121 tgatgaactg gcctttcccc ataccaatct caatgtggat tggatgatcg ttttcaaaca
     9181 attcattcca ttttcccttc cacaatgttg gatcactaat tacaaaattc gggtgagccg
     9241 ctaacatttc cgctgctccc ggacgatttc ggacacgcat tatttatctc cattcctttc
     9301 ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg ggccaaacct cagccactga
     9361 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
     9421 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
     9481 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
     9541 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
     9601 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
     9661 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
     9721 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
     9781 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
     9841 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
     9901 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
     9961 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
    10021 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
    10081 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    10141 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    10201 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    10261 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    10321 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    10381 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    10441 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    10501 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    10561 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    10621 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    10681 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    10741 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    10801 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    10861 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    10921 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    10981 tgcccatatt agccaataaa atgatagaaa caaaaagatc agagaagaaa aaaaccagcc
    11041 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    11101 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    11161 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    11221 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    11281 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    11341 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    11401 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    11461 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    11521 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    11581 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    11641 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    11701 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    11761 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    11821 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    11881 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    11941 ttttcattgc tatatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    12001 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    12061 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    12121 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    12181 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    12241 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    12301 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    12361 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    12421 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    12481 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    12541 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    12601 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    12661 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    12721 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    12781 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    12841 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    12901 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    12961 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    13021 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    13081 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    13141 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    13201 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    13261 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    13321 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    13381 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    13441 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    13501 gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    13561 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    13621 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    13681 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    13741 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    13801 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    13861 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    13921 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    13981 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    14041 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    14101 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    14161 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    14221 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    14281 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    14341 tcttcaactg aagaaacaac tgattcatca aaataaa
//
LOCUS       DAYYAJ010000072        14223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000072 DAYYAJ010000000
VERSION     DAYYAJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.072"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..2030
                     /gene="yfcC"
                     /locus_tag="ACHATZ_001831"
     CDS             291..2030
                     /gene="yfcC"
                     /locus_tag="ACHATZ_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8404352.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2187..3371
                     /gene="iadA"
                     /locus_tag="ACHATZ_001832"
     CDS             2187..3371
                     /gene="iadA"
                     /locus_tag="ACHATZ_001832"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8404353.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3482..3685
                     /locus_tag="ACHATZ_001833"
     CDS             3482..3685
                     /locus_tag="ACHATZ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8404354.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3786..4448)
                     /locus_tag="ACHATZ_001834"
     CDS             complement(3786..4448)
                     /locus_tag="ACHATZ_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404355.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4461..5963)
                     /locus_tag="ACHATZ_001835"
     CDS             complement(4461..5963)
                     /locus_tag="ACHATZ_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404356.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6511..8190
                     /locus_tag="ACHATZ_001836"
     CDS             6511..8190
                     /locus_tag="ACHATZ_001836"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8404357.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8317..8796
                     /locus_tag="ACHATZ_001837"
     CDS             8317..8796
                     /locus_tag="ACHATZ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8404358.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9015..9695
                     /locus_tag="ACHATZ_001838"
     CDS             9015..9695
                     /locus_tag="ACHATZ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8404359.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9703..11034
                     /locus_tag="ACHATZ_001839"
     CDS             9703..11034
                     /locus_tag="ACHATZ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8404360.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11049..12011
                     /locus_tag="ACHATZ_001840"
     CDS             11049..12011
                     /locus_tag="ACHATZ_001840"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8404361.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLASGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12122..12460
                     /locus_tag="ACHATZ_001841"
     CDS             12122..12460
                     /locus_tag="ACHATZ_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8404362.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12539..13246
                     /locus_tag="ACHATZ_001842"
     CDS             12539..13246
                     /locus_tag="ACHATZ_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8404363.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13349..>14223)
                     /locus_tag="ACHATZ_001843"
     CDS             complement(13349..>14223)
                     /locus_tag="ACHATZ_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8404364.1"
                     /translation="NIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFT
                     SFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMT
                     YLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQE
                     KKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQS
                     HLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHE
                     LYMKKGYENKTKRM"
BASE COUNT         4564 a         2469 c         3066 g         4124 t
ORIGIN      
        1 ttttgcaatg aaattcaatt tttcaatgat tgtaaaatga aaaaaacagg acgagatttt
       61 ctctaatcta aaaataaaaa aagaatttta aggaaaaaca gagaaataaa atacttaata
      121 taaatggaaa gagaaaaaaa taatcataca taggagaaaa aaaacaaaat agaaaaaagt
      181 gattttctaa taaattattc atgtggaaat gtcattttta ctgtataatt catttgatta
      241 tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat ttgaagaaaa
      301 caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc catacgtatg
      361 tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt ccagcaggaa
      421 agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt actcgtactg
      481 ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat gtgtttgatc
      541 aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt ccggaaaaag
      601 gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa
      661 tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat gatacttcta
      721 aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc tttggtttct
      781 taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct gtagggatcg
      841 ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg
      901 acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta gctttaccgc
      961 tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa gaggtaattc
     1021 cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg atcgtagcag
     1081 taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg agcccgttta
     1141 gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg acattccgat
     1201 tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata tatgcagaaa
     1261 aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat ttccgttctc
     1321 atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa ttgatcttat
     1381 tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa ggctattaca
     1441 ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc attgctgttc
     1501 ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa tctggtgcag
     1561 ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt cttgtcttgg
     1621 gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg atcgggacgg
     1681 cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc caaagtttgt
     1741 tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca atcatggctc
     1801 cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat cagcttggtt
     1861 caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg ctaggtgttg
     1921 cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg
     1981 ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag tactggagac
     2041 ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt cattttacgg
     2101 taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc tatcatcagt
     2161 tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa gtgtttgcac
     2221 cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg gctgtagaag
     2281 aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt gagggaaaag
     2341 tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga ggagaaggcg
     2401 gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct ggtgtaacga
     2461 cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg ttgatcagca
     2521 aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt tcgtaccgat
     2581 tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt gataaaatta
     2641 tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt tttgaagagt
     2701 tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa gctgggatca
     2761 tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga atcgtagaag
     2821 aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca ccagaattat
     2881 ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact gctagcgaag
     2941 atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca ctgaaacgat
     3001 tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga caaggaagct
     3061 tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt gccaaggcat
     3121 tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa atcgccttac
     3181 gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa
     3241 ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt gatacagtta
     3301 tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg actttcgaaa
     3361 aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc tgtttgtctg
     3421 tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag gaggaagcac
     3481 aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa
     3541 agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga aagcacaatc
     3601 actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca aagacaagtt
     3661 ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca agcaaataaa
     3721 aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga tcattatcct
     3781 gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac ttcatcacag
     3841 ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg ttcatgggcg
     3901 atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt tccagtaggc
     3961 tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc gactcgctgc
     4021 tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc aattcctaca
     4081 cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt agtgatttcc
     4141 attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta agcttggaaa
     4201 ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag tgaacgatct
     4261 tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga aagaaaagtc
     4321 gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc gaaattcagt
     4381 tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc
     4441 cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa tcttaatatc
     4501 cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc ggctaatagt
     4561 cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga aacagcaaat
     4621 ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg tctgttacct
     4681 ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca
     4741 agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc aactcctaat
     4801 gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt acttctggat
     4861 tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt aatcataatg
     4921 atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt tttagaaaaa
     4981 cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt tgattgtaag
     5041 ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc cattttatct
     5101 ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt cccatttagt
     5161 tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa gttgaatttc
     5221 atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt catttcataa
     5281 gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc caggtcattt
     5341 gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt
     5401 ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg tgcagttgct
     5461 gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc cattcctcca
     5521 gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc agaactagtg
     5581 atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga ataactcttt
     5641 acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt aagagaaaaa
     5701 ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt
     5761 ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc tttttgtgct
     5821 tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac aaaaataaga
     5881 atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt tgtacttttc
     5941 catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat ttgataatag
     6001 acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca acatgaggtt
     6061 ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat
     6121 gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct agatgctaca
     6181 gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat ttagtttatt
     6241 tgttgtataa taatggggaa cagctgaata cttatataag tattctggct atagcgtttc
     6301 gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc caactgtcag
     6361 aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa tagtgatttt
     6421 gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg acatctttaa
     6481 acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat cccattaggc
     6541 ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga tatttttatt
     6601 ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct
     6661 gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt aacacatgga
     6721 catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt accagtattc
     6781 gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa
     6841 ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg tgaaacagta
     6901 gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa tctgaaaaca
     6961 aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc tatcccaatg
     7021 tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt agccttacta
     7081 agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca aatagcagat
     7141 gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt
     7201 aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg aaaagttgtc
     7261 ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga aaaacttcaa
     7321 ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga tccagaagaa
     7381 ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca aaaaatggca
     7441 aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat cactactacg
     7501 ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta tcgagctggt
     7561 ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa tcctaatgac
     7621 ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca aggagagtat
     7681 cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata caaaaatatc
     7741 tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag tgttgcagga
     7801 actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat cggaaacatt
     7861 gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt tgtcactatc
     7921 agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg attcgtttac
     7981 gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac aatcgttgag
     8041 aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat ccgagatcaa
     8101 ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc agtcattatg
     8161 gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac ctctctatag
     8221 agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca gcatccctcg
     8281 ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac aaaagtcgaa
     8341 cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca tgtggtagaa
     8401 gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat atgtaaaact
     8461 ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt gaacgatcac
     8521 ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt tcctggaatg
     8581 gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat tggaataaaa
     8641 ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa agaagaagta
     8701 attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga tttagaaaag
     8761 atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga agctaagcag
     8821 agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc tgtattatct
     8881 gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt tagcagctat
     8941 attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata cagagaaaac
     9001 gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca attgaaatgg
     9061 aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg acagacttaa
     9121 ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt gaccacatag
     9181 gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt gttgagattg
     9241 ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt ggaaaacatg
     9301 cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc tatgaagtag
     9361 acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca gaaggaaaaa
     9421 cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt gcagaagaat
     9481 tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg ccagaagatg
     9541 cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat tacaatactt
     9601 tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat agtgaaggaa
     9661 aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac aaaacatgat
     9721 ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt aagaagcatc
     9781 gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga tgcagaaaat
     9841 gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg caaattaaaa
     9901 aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg ggacgtttta
     9961 ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc gatgactaaa
    10021 catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa ccgggcggaa
    10081 gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg attcaatacg
    10141 actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg cacctcttat
    10201 gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt gatcaaaaaa
    10261 gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta tttgcagcag
    10321 acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc gcgttttcca
    10381 gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt tttagggaaa
    10441 tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt aaagaaaaca
    10501 atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt catgccggtc
    10561 gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat gaaagcgatg
    10621 cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat atctggaaat
    10681 atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa gttcaaattc
    10741 gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt acttagcgaa
    10801 atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa tatcttgatt
    10861 gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt agacattcgt
    10921 ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt gtttattgaa
    10981 aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc ttagaaagca
    11041 ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac gataaaatca
    11101 ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta ggattagccg
    11161 aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa acgattgaag
    11221 ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa ggagcgctag
    11281 tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat cagttggcag
    11341 aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat cttgtgtggg
    11401 taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg atgtttcctg
    11461 aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc gtcggagata
    11521 cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct tcgatattga
    11581 gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg ccgatgaaag
    11641 tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat ggtgcaggaa
    11701 gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca gagacagctt
    11761 cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttcggggatt tttgttcagc
    11821 agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc aatgaattgg
    11881 ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca aaagatttag
    11941 cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat aagagcagaa
    12001 aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac aaatgtatct
    12061 tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa ggagaaagaa
    12121 gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag atcgaggctt
    12181 gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa ctgtcataga
    12241 agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg atagcgtgac
    12301 agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag gaacaaaagt
    12361 caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag aaggatttgg
    12421 accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca aggtcttttc
    12481 acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg aaacgaaaat
    12541 gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca ttcttggatt
    12601 agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat acgatcaagt
    12661 gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac aaagtcaaca
    12721 gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact acaattcagg
    12781 taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg ctgcgatagg
    12841 tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg tcccattgaa
    12901 gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt tccccttgat
    12961 tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga ttttcgcttt
    13021 ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg cgtctcggcg
    13081 agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac cgatggcaag
    13141 acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga cattcctgca
    13201 gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata aagaagttgt
    13261 gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata ccatttattt
    13321 ggaataagag gatgtgacaa gaattttgtc acatcctctt tgttttgttc tcataacctt
    13381 ttttcatata cagttcatga accgctttta ttaacttgcc aaataacact gtattgtgcg
    13441 ttgtatccag atagaaagtc tccgtttcat ctgaaatttt ctcatgctga tctgtaatga
    13501 tcaaatcagc atgttctgaa tatgcggtaa ttttaacagc atctttgcga aataactgct
    13561 gcaaatggct ttgaagatga taatttcctg taaactgttt ggttatctgc aaatggatga
    13621 gtaactgctc tttcgtttct gtttgtagta agtgttggat caattgacta aggtattgac
    13681 tgatttggtt tctttcccca ttttttgaaa aaattgtatc gattttttct cttaatttct
    13741 tttcctgtat aaaagaacta gaatgattgg acgtggcttc tggaaagcaa agtgtagaaa
    13801 aactactgct ggattcaccc aacagttcga aaaaaacaac tgccaacaca agaaaataaa
    13861 aataaattgg tattttattt tctaaatggt tgagaggaaa ctgttgagtt aagtaagtca
    13921 tcaattgacc gacttgtcga caaataaggt ttttgcttgt ggaaaataaa aaaccaatct
    13981 cctgtttttg tcgaaaagag atttgatttg gaaaaaaaca atagaaaaaa taattaaagt
    14041 aggctctctc tgtttctgtc tcttcacaag ggcgactcac tgaaaaagaa gtaaagtatt
    14101 ctgaataatc atgtgtatgc tgcaataagt ccatcaattc agtgatttca gaagaagttt
    14161 ttggaaaata tgcctgttgt ttgtttcgta atatgaataa agttaacagc aagtagatat
    14221 ttt
//
LOCUS       DAYYAJ010000073        14024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000073 DAYYAJ010000000
VERSION     DAYYAJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.073"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..765
                     /locus_tag="ACHATZ_001844"
     CDS             64..765
                     /locus_tag="ACHATZ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8404365.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="ACHATZ_001845"
     CDS             762..1883
                     /locus_tag="ACHATZ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8404366.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="ACHATZ_001846"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="ACHATZ_001846"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8404367.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="ACHATZ_001847"
     CDS             3262..4170
                     /locus_tag="ACHATZ_001847"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8404368.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="ACHATZ_001848"
     CDS             4205..4477
                     /locus_tag="ACHATZ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8404369.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="ACHATZ_001849"
     CDS             4488..5534
                     /locus_tag="ACHATZ_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8404370.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5785..8394
                     /gene="clpB"
                     /locus_tag="ACHATZ_001850"
     CDS             5785..8394
                     /gene="clpB"
                     /locus_tag="ACHATZ_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8404371.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8545..9231)
                     /locus_tag="ACHATZ_001851"
     CDS             complement(8545..9231)
                     /locus_tag="ACHATZ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404372.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9209..9703)
                     /locus_tag="ACHATZ_001852"
     CDS             complement(9209..9703)
                     /locus_tag="ACHATZ_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8404373.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9723..10943)
                     /locus_tag="ACHATZ_001853"
     CDS             complement(9723..10943)
                     /locus_tag="ACHATZ_001853"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404374.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11090..12925
                     /gene="lepA"
                     /locus_tag="ACHATZ_001854"
     CDS             11090..12925
                     /gene="lepA"
                     /locus_tag="ACHATZ_001854"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8404375.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13121..>14024
                     /locus_tag="ACHATZ_001855"
     CDS             13121..>14024
                     /locus_tag="ACHATZ_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404376.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIY"
BASE COUNT         4676 a         2482 c         2912 g         3954 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa gaaaaacgtt
     5581 atggataaga taattttacg tttgtttggc tcttttttta tatacataca atcaattttg
     5641 tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac
     5701 caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac
     5761 ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa
     5821 gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc
     5881 catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc
     5941 ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt
     6001 gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg
     6061 aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt
     6121 attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga
     6181 ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc
     6241 tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag
     6301 caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc
     6361 atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta
     6421 gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa
     6481 aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa
     6541 ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat
     6601 ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa
     6661 ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg
     6721 atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa
     6781 cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg
     6841 cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta
     6901 gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca
     6961 atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg
     7021 gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa
     7081 aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa
     7141 cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat
     7201 tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa
     7261 aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa
     7321 aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag
     7381 tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg
     7441 aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga
     7501 gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct
     7561 ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc
     7621 ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg
     7681 gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct
     7741 cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat
     7801 ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc
     7861 ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc
     7921 aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg
     7981 actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga
     8041 aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc
     8101 ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa
     8161 catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct
     8221 tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca
     8281 ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact
     8341 ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc
     8401 attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca
     8461 aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc
     8521 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
     8581 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
     8641 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
     8701 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
     8761 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
     8821 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
     8881 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
     8941 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
     9001 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
     9061 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
     9121 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
     9181 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
     9241 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
     9301 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
     9361 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
     9421 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
     9481 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     9541 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     9601 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     9661 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     9721 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     9781 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     9841 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     9901 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     9961 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
    10021 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
    10081 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
    10141 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
    10201 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga
    10261 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
    10321 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
    10381 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
    10441 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
    10501 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
    10561 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
    10621 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa
    10681 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
    10741 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
    10801 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
    10861 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
    10921 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
    10981 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
    11041 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
    11101 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
    11161 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
    11221 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
    11281 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
    11341 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
    11401 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
    11461 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
    11521 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
    11581 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
    11641 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
    11701 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
    11761 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
    11821 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
    11881 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
    11941 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
    12001 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
    12061 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
    12121 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
    12181 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
    12241 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
    12301 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
    12361 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
    12421 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
    12481 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
    12541 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
    12601 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
    12661 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
    12721 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
    12781 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
    12841 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
    12901 ggatgagcaa gatagtgtga aataacttat aatcaatcga aaaacctatt aaataaacct
    12961 ttttgagtag atgcaacact gaggattatc tgtataaaag attttaaact gtcatttggt
    13021 tatcacggcg atactaaaat taacaaaatt ggaatatttt tcttgaagat ttttagctgt
    13081 cagtatatga tgaaagcatc tcataggagg tgcttttttc atggttgata attcaagtgg
    13141 aagggttctt aaatctctaa gaaaagagaa aaaactatct caaaaaaagt tagctgattt
    13201 agcaggtatt tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa aaatccctaa
    13261 agacgtagat aatactctaa gcaagattct gaatatagaa actctaataa aagatgagga
    13321 agataagatt gagattctca tcggtaaact aattgcatac agagatatga ataagttact
    13381 taacaaagag ttagctgata atataggaat tagtgaagta ttactcagtt atgtgttaaa
    13441 tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc gatatttttc tattaagtaa
    13501 tgaaaaagaa attttgaagg agattaatcg tgatagtgag atatttagtt tatcaaaaga
    13561 cgataaaatt gttatgggaa aaagaatacg agaagtccga aagaaccggg aagagacgct
    13621 tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa aatgttataa gtagatggga
    13681 aaaaggtatt aatattccag atattgaaaa actaatgaat attgcctatc ttggtaaggt
    13741 cactgttcct taccttatgt acggagaaga ttataagaat atacttccaa aagatgaaag
    13801 agttagcgat tttaaaaaga taaattcttt tagtatggga ttaaggatga ggaaaattag
    13861 gaaagattat tatctagaaa gggaggagtt tggaaagctt ttttctcctt caatttcaaa
    13921 atggtccata gataggtatg agaatggtag agatataccc aatactaatc gaattatcca
    13981 atatgcttat ataggaaatc tttctttaga atttttgatt tacg
//
LOCUS       DAYYAJ010000074        13921 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000074 DAYYAJ010000000
VERSION     DAYYAJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.074"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACHATZ_001856"
     CDS             complement(146..1111)
                     /locus_tag="ACHATZ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8404377.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACHATZ_001857"
     CDS             1345..2190
                     /locus_tag="ACHATZ_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8404378.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACHATZ_001858"
     CDS             2381..3568
                     /locus_tag="ACHATZ_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8404379.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACHATZ_001859"
     CDS             3574..3930
                     /locus_tag="ACHATZ_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8404380.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACHATZ_001860"
     CDS             3932..4270
                     /locus_tag="ACHATZ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8404381.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACHATZ_001861"
     CDS             4665..5699
                     /locus_tag="ACHATZ_001861"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8404382.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACHATZ_001862"
     CDS             5692..6378
                     /locus_tag="ACHATZ_001862"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8404383.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACHATZ_001863"
     CDS             6402..7250
                     /locus_tag="ACHATZ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8404384.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACHATZ_001864"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACHATZ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8404385.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACHATZ_001865"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACHATZ_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8404386.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACHATZ_001866"
     CDS             9516..10751
                     /locus_tag="ACHATZ_001866"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8404387.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACHATZ_001867"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACHATZ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8404388.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACHATZ_001868"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACHATZ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8404389.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12702..13844)
                     /locus_tag="ACHATZ_001869"
     CDS             complement(12702..13844)
                     /locus_tag="ACHATZ_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8404390.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4529 a         2575 c         3007 g         3810 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tgggccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaacc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg
    12601 ttaataacta tttcattgct cgaaacgttg atattaaagc gtttcgagcg tttttttatt
    12661 tgtattttaa tggttggcta cgtttttggc tacgtttgga attattcgcc cataaatttt
    12721 gcaaatcggt caccagtttt cttaacagat tgtggtgtga catgagcata gatattcatg
    12781 gtagttttaa tatctttatg acctaatcgt tcctgtactt cttttatcga cgcaccactt
    12841 tcaaacaata agctacagtg ggtatgccta aatccatgtg gagtaatttg aggctccatc
    12901 ggtgtctttt tgtaaatcca gtctaaccaa tcgtttacta cttgggggta gtacaacttg
    12961 tttgtgtttg tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc atataagatc
    13021 attattttca tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa atcatcaagc
    13081 tgaatcgttc ttgatgaatt tttagtttta ggtacttgta gaactacttg attaaactcg
    13141 tcttgagcga tagttttacc aattgttatt gttttattga aatagtctat atcttcccat
    13201 tgaagcgcca atgcttcgct tttacgcatt cctgtataag ctaataaacg gaaaaaagtg
    13261 tacagtttaa ctgaattatt ttctttagta ttctgtaaaa acagttgtaa ttgatctttc
    13321 gtgtaaaact tcaagggttg ttcttcttca atttttcttg gaagcaatgt tttagacata
    13381 ggattatcct tacagacttc cattgctact ccaaatttaa atatttgagc agttgttttt
    13441 ctcaagaaac catattgttt taatgttttg tgccattcat tcacctgttt ttggcaatag
    13501 ctaacactta ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg gtttttgcaa
    13561 aaacgttgag caacggcaac agaagacggt tttactcttg ttctgtagtt atcaatccat
    13621 aattcataca actcttgaaa agttgtaaca ttgattttaa actcttgttt ttctttaaag
    13681 tctacaagta gtctattaag tgctgtttgc gcctcagctt ttgatttgaa tcttctacgc
    13741 tttgtacgta tatcttttcc agtcttggga tctttcccta aataagtttc aaatccccaa
    13801 tatttttcgc cattttgtaa tttataacta aaaaatgttg ccatttgatt ctctccttac
    13861 ttcattattg caggtagaga agttatttga agaatcaatt atttttaaat ttatcgtgat
    13921 t
//
LOCUS       DAYYAJ010000075        13898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000075 DAYYAJ010000000
VERSION     DAYYAJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.075"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHATZ_001870"
     CDS             complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHATZ_001870"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8404391.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHATZ_001871"
     CDS             complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHATZ_001871"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8404392.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHATZ_001872"
     CDS             complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHATZ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8404393.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHATZ_001873"
     CDS             complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHATZ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8404394.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5395..5790)
                     /locus_tag="ACHATZ_001874"
     CDS             complement(5395..5790)
                     /locus_tag="ACHATZ_001874"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8404395.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHATZ_001875"
     CDS             complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHATZ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8404396.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6267..8459)
                     /locus_tag="ACHATZ_001876"
     CDS             complement(6267..8459)
                     /locus_tag="ACHATZ_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8404397.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8483..9454)
                     /locus_tag="ACHATZ_001877"
     CDS             complement(8483..9454)
                     /locus_tag="ACHATZ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8404398.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHATZ_001878"
     CDS             10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHATZ_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8404399.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11693..12139)
                     /locus_tag="ACHATZ_001879"
     CDS             complement(11693..12139)
                     /locus_tag="ACHATZ_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8404400.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHATZ_001880"
     CDS             complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHATZ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8404401.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12504..12932)
                     /locus_tag="ACHATZ_001881"
     CDS             complement(12504..12932)
                     /locus_tag="ACHATZ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8404402.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13024..13884
                     /locus_tag="ACHATZ_001882"
     CDS             13024..13884
                     /locus_tag="ACHATZ_001882"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8404403.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3822 a         2881 c         2501 g         4694 t
ORIGIN      
        1 ttcataggtg tttttaaaag ttacacaaaa aataaaataa caaaaaaatt aacattgaat
       61 taataatagt tatttatatt tataacaaat aaaccagaga aatagcctat tttgtgcctg
      121 ttctctggtt tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag
      181 tgacaaagcc atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg
      241 ttcttctgcc atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac
      301 aagctgatca taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac
      361 tgctttgtgc agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg
      421 tttttcaagc tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg
      481 tttgaagtct tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt
      541 cagatctttt tgttctgcat tcaaaacagc ttcgatgaca tcatgacgga tattcttcgt
      601 tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga
      661 caagccaatg ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag
      721 aacagacaaa ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg
      781 acggcgcaaa gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga
      841 aaagatacta tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtaa
      901 atctccttca ctggatatag gcatgtaatg ttcacggatc gctgttgcta cggctggttt
      961 ctcgccttta agcagagcat atttttcgcc catgatccct tgtagttcag ggaattctcc
     1021 taccatgttg gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa
     1081 ttcttcctca gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg
     1141 cattttctca tagatgctac cgattttttc atggaaagta acagttttta atttttcaac
     1201 gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg
     1261 agcagtcaat actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt
     1321 acgagcagaa ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg
     1381 ttgatgttct ttcatagaag tgactagtac ttcttcagga acagatagat atttttcttc
     1441 gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt ctagtaaatc
     1501 ttcatctaga tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtgatgat
     1561 catttctttt cttttttgcg gtgaagcaat aacaaattgt tcattcaatt tttcttcgta
     1621 ttgttttgca tgcgtaaatg tcacatcttc gcctaagaaa cgatgacctc ttgaagctct
     1681 gtctgttttg acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat
     1741 ccagtgaata gggcgaatat attcaaaatc gtattctgcc cagtgcatcg ttactgggaa
     1801 agtgaggctt ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg
     1861 agtgtatttc gttacataga catattccac gccttttaat tctctaaaag tgatagcatc
     1921 tgtcgtcaga ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc
     1981 gtccaaagca atttttttgg ctggtccttt gacttcttct tcgctgtctg cttgacgttc
     2041 tggaatgttt gttactttga ttgctaagcg gcgcggtgta gaaaacgttt cgatcgtatc
     2101 ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct
     2161 tggtgtgact acatgagcag gtatttcttc cagcccgatt tcaagtaata agtcttttgc
     2221 catcttattc agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga
     2281 atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga
     2341 tacgggaaag ataacctgca cgctctgtta cggatactgc accgcgagca tctaataaat
     2401 tgaatgtatg gctacatttc aaaatataat catacgctgg atgaacaaga ttttcatcga
     2461 tacagcgttt agcttctttt tcaaaacgat caaaattgct tagtagcagg tcttggtcgc
     2521 ttacttcaaa agaatattta gaatgttcat attctggttg tttgaagatc tcaccatatt
     2581 tcacgccatc agcccattcc agatcataaa cactttcaac ttcttggata taagaagcaa
     2641 gacgttctag tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt
     2701 gctggaagta tgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac
     2761 cggcacaacc catcgatggg ttctcccagt tgtcttcaac aaagcgaata tcgtgttcta
     2821 atggatcaat tcctaaaaga cgcaagcttt ctaaataaag ctcttggata ttttcaggtg
     2881 aaggtttcat tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat
     2941 aacgcccgtc agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc
     3001 cgattgcccg taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag
     3061 cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt
     3121 ggacggtcaa ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa
     3181 taagaataca ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg
     3241 aataatttcg cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac
     3301 tcgcaagtgc ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa
     3361 ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttctggtttg
     3421 tataatagat cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc
     3481 cctgtatttg tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc
     3541 aggtatctct ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt
     3601 catttgatca atgaactttt tgctttttag atgaataccg acatattcat catacaactg
     3661 gtcaatgagg tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt
     3721 atcataagaa atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata
     3781 acggtgttcg tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata
     3841 gtcaaatttt ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc
     3901 gaagcgttgc agtatttgca cttcaaagat attcgtgatg atttctgcat catctccttg
     3961 gtccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc
     4021 ttcgattgct gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc
     4081 ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc
     4141 acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg
     4201 ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa
     4261 gattttgact aatttatctt tttctttgta attacggcta gataaaatca agcctttcga
     4321 ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc
     4381 cgatccagat tatttatgtt tcgatcttcc gctgtttttc tcaacgtacg gatttttatc
     4441 cagtttattt ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt
     4501 agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa
     4561 gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta
     4621 cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta tctctttcga
     4681 cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa
     4741 cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca
     4801 attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg
     4861 ggccctctgg catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg
     4921 tagcagagat cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc
     4981 ccagcaactc gtctggatgg atcttatcga ttttattgat aatcagataa acaggagaat
     5041 gcacgttttt tagacgttca ataataaaat cgtctccccg gccacgtttt tgatcagcac
     5101 tgatcataaa taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa
     5161 aatctcctag acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag
     5221 cttctggtgt agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca
     5281 taatggcaat tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc
     5341 ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt
     5401 aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca
     5461 agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa
     5521 atgaaaatcc gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat
     5581 ccaaacgaga aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat
     5641 cacgcccata aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc
     5701 ctcaaaaacg gttttgactc cttggacagc aaattccaca gacatcagga aatgtttgtt
     5761 tttctctacg tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa
     5821 acatttcttt ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat
     5881 gtaccgctaa aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag
     5941 cagcacgttc aatagagatc atgatgtctc ctagtacacg aggtaatgga ttctctccat
     6001 cttcaaaaat cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt
     6061 ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca
     6121 tgaaagtaac agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca
     6181 gcaagtcatc gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg
     6241 taatatccat tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact
     6301 tctgttttca ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatt
     6361 aatgattttt ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca
     6421 tcggaaatac gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga
     6481 tgatccattg cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca
     6541 cgactttgcg gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt
     6601 tctttcgctt tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata
     6661 tcgatcacca tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt
     6721 ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct
     6781 gaaggcaagt tttcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag
     6841 caagctactc gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga
     6901 ttagctacca tcatactatg atgataagta cctggcgctt cttccagcaa ctgtttcaat
     6961 aaaggatgat ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc
     7021 aattcaatat atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac
     7081 ccgcagacga gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc
     7141 aaaatgacat ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt
     7201 tctgagatcc gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc
     7261 aagataatcg ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact
     7321 gcttggaaaa taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt
     7381 ggtgcaaaag cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat
     7441 tggaaaaatt tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat
     7501 tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga
     7561 agcaaaacag ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt
     7621 cctagtaatt ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg
     7681 atttctcctt ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct
     7741 tctgtctttt tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga
     7801 atcatttgtt gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt
     7861 ttaaaagctt ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc
     7921 agactctcat ccctcacagt atgaatctca gtttcagtca tttcaaatat cgtcgaataa
     7981 aaattgttcg gtagtttttg ataaaacaca acattttctg cattcacatt ttcaaaattt
     8041 ttcttcagcg cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt
     8101 gccttatcaa tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat
     8161 tcaaatagtt gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat
     8221 tctggagtga ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg
     8281 atcgttttgt tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc
     8341 ttttgtttga cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg
     8401 aaaggcaatg ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg
     8461 tgcttatcct cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga
     8521 tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga
     8581 ttttcttgat ttgctttaat gttcgttctg catgaacaag accacttgtc gtccctcgag
     8641 gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa ccgagacggg
     8701 tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg
     8761 catcatccaa tgttcgcccc cgcatgtaag ctaaaggtgc gatttcgatt acgcctcgtt
     8821 ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca taaacaggcc
     8881 gcagataagg atccactttt tcttttagat ccccgggaag aaagcctaga ttttcacctg
     8941 cttccacagc aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga
     9001 ttgctaatac tactgcaaga aaagtttttc ctgtaccggc tggtcctacg ccaaatacga
     9061 tatcatgttt ggctaccgat tcgacatatt tcttttggcc gctgtttttt acccgtatag
     9121 gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg
     9181 tacctttttg ccccattttc aatgccgtca caacatcggg cgtacttacg tggattcctc
     9241 gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc tgggattgct
     9301 caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt gtttcctcaa
     9361 tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca
     9421 atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt
     9481 cattctttcc tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta
     9541 aaagagttgc ttaaccataa gtaccaagaa acgttaaaaa actagccgct tccattctct
     9601 aaaggtctcg tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa
     9661 gtattccgtc taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa
     9721 caaaatattt gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa
     9781 taaatataca gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga
     9841 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
     9901 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcgaaa
     9961 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10021 gagccctttt cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg
    10081 aatctgatat tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata
    10141 attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat
    10201 ggtcagttca cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtaca
    10261 cttaggtcaa atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg
    10321 cgctcttttc ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta
    10381 tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt
    10441 aacgatcgga ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc
    10501 taaacccttt ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt
    10561 ctttttactt gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa
    10621 gtatttaaac tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca
    10681 acccatgggg aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa
    10741 tggcgtttta gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac
    10801 ttttgtccat gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc
    10861 aaaagctggt agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct
    10921 cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca
    10981 gatcactaat ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt
    11041 aggtgtcttc actactgcta tgggactcgt tgtttctttt gcacaagatt tccataaact
    11101 gttccctaaa gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt
    11161 tgcgaatgcc ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta
    11221 tccattgtca cttgcgttaa tcttactttc cttaacagca aaattcttcc aaaagagtcc
    11281 atttgtctat caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc
    11341 aaattcgcct gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca
    11401 tcattatgtt ccttttgctg cattaggtct tggctgggtc gtcccaacgt tgcttggtta
    11461 cgctggcagc ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag
    11521 tgagttacca gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
    11581 aaaaaacagc tagaagaaag aataagattc tttcttctag ctgtttttta gttctttttt
    11641 tacgaacaca aaaaactgcg gataatcact gtccgcagtt taaatgataa aattaagatt
    11701 gtaatagttc tttcacaatc gcattcactt ggtttccatc tgcttttcct ttgactttag
    11761 gcataattgc acccataact ttgccaaatt ctttcattga tgaagcacca gtttgagtga
    11821 ttgcttcttg aacaagttgt cgaatttctt cgtcggaaag ttgttccggc atatattttt
    11881 cgactattac aatttcactt tttacttttt cagctaaatc gtcacgtcct gcttcttcaa
    11941 actcatgcaa agaatcacgt cgctgtttca tttcacgaga caacaccgtt agttcttctt
    12001 cttctgtcaa gtcgtgacct tctttaatct gctcgttttg tatagatgac ttgatcattc
    12061 ggatgacttg taaagattcc ttatctttcg ctttcatcgc tgctttcatg tcatcgttta
    12121 atctacttag tagtgacaca ctaccaactc caattcatca cagaactaga attttttgcg
    12181 ttttctagct gcttcagatt ttttcttgcg tcttacactt ggtttttcgt agaattcacg
    12241 tttacgagat tcttgtaaag tacccgcttt tgacacggaa cgtttgaagc gacgaagagc
    12301 atcgtcaagt gattcgtttt tgcgaacgac tgtttttgac atgttaattc cctccctccg
    12361 agcttaaaaa gtaaccgtaa ttgttattga aatatatctc aaaaaacaag actgtcctgt
    12421 aaatattata tctaatgctg ttttgaccgt caaggaaatc ttgctttttt ttaaaattat
    12481 caaatgttat tttatttaaa tccttattga caattttcac atcttccgaa aatctcgaat
    12541 ctatgccctt cgatcgtgca tccacctagc tgttcttcga aataattcat cggacacata
    12601 tggatttctt tcgttgttcc gcacactgta cagataaaat gatggtgatg gccgattttc
    12661 ccctgacagc aatgaaaacg aaacttcatt tctccattta aatcggtttc ttccagtaaa
    12721 tcgatctctg caaaatcatg gagattccgg taaatcgtat cataacttac ccctttgaat
    12781 tcctgattca tatagtcaaa aacttcttta gcagatacgt agcggttacg cttcactaaa
    12841 taagttaaca atgcttctct ttttttagta tatttaaaac cgttattttt caatgtttct
    12901 aatgattgtt cgatcaatgt ctgttttttc atgacttatt cctccaaatc aaaatgatta
    12961 cgaagtacgc attctagagt atggtataat aaacataaaa aaaatgcaag tgaggtttta
    13021 tccatgtctt ccgattctac agaaatcgtt actttttttg tcatctccga ctctgctgga
    13081 gaaactgcga caaaactagc gcaagctact atggctcagt atcctaccgt tgagttcaac
    13141 ctattccgca gaacgttcgt gacagatgaa aaaacattaa ggcaggcatt gcaagatgca
    13201 ttagaagaaa aagcacttgt cctgcataca ttaatcaatc aagaactgat cgagataaca
    13261 cgttcttttt gtgaagaaca tcaattattt agttttgatg tgatgacgcc accgatatca
    13321 gaaattgaac gtttaactgg tatcaagcca attcggcaac ctggtgccct ccatttattg
    13381 aatgagaact atttcaaacg gatcaaagca atggaatttg ctgtaaaata cgatgatggg
    13441 aaagatcctc gtggtttttt agaagcagat gtcgttttgc taggcgtatc ccgaacttcc
    13501 aaaacgcctt tgagcttgtt tttagctaac aaaaatttaa aagttgccaa tttacctttg
    13561 atcccacaag cacatattcc caaacagctt tgggagatcg atcctaaaaa aatcgtggga
    13621 ctaacgaata acccagatat tttaaataat atccgtaaag aacggatgcg ctcttacgga
    13681 ttaaaccctg atactgctta ttccgatatc gaaaaaatta gagctgaact tgattttgcg
    13741 aatgatcttt atgaaaaact aggatgtgta gtgatcaatg tggcatcttt atctattgaa
    13801 gaaacggctt cgcttatttt gaatgcttta gacctagaag atcatagtta ttatggaact
    13861 gaaacaacag aaaaaagcga ttaatcattt ttgtcttt
//
LOCUS       DAYYAJ010000076        13862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000076 DAYYAJ010000000
VERSION     DAYYAJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.076"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..2302
                     /gene="acm"
                     /locus_tag="ACHATZ_001883"
     CDS             137..2302
                     /gene="acm"
                     /locus_tag="ACHATZ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8404404.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2574..3059)
                     /locus_tag="ACHATZ_001884"
     CDS             complement(2574..3059)
                     /locus_tag="ACHATZ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8404405.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3149..3494
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3539..4180
                     /locus_tag="ACHATZ_001885"
     CDS             3539..4180
                     /locus_tag="ACHATZ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8404406.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4199..4834
                     /locus_tag="ACHATZ_001886"
     CDS             4199..4834
                     /locus_tag="ACHATZ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8404407.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4852..5667
                     /locus_tag="ACHATZ_001887"
     CDS             4852..5667
                     /locus_tag="ACHATZ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8404408.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5986..7422
                     /locus_tag="ACHATZ_001888"
     CDS             5986..7422
                     /locus_tag="ACHATZ_001888"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8404409.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7611..10043
                     /locus_tag="ACHATZ_001889"
     CDS             7611..10043
                     /locus_tag="ACHATZ_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8404410.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10273..11340
                     /gene="rlmN"
                     /locus_tag="ACHATZ_001890"
     CDS             10273..11340
                     /gene="rlmN"
                     /locus_tag="ACHATZ_001890"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8404411.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11330..11536
                     /locus_tag="ACHATZ_001891"
     CDS             11330..11536
                     /locus_tag="ACHATZ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404412.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11533..12636)
                     /locus_tag="ACHATZ_001892"
     CDS             complement(11533..12636)
                     /locus_tag="ACHATZ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8404413.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13188..13736
                     /gene="aac(6')"
                     /locus_tag="ACHATZ_001893"
     CDS             13188..13736
                     /gene="aac(6')"
                     /locus_tag="ACHATZ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8404414.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4749 a         2298 c         2935 g         3880 t
ORIGIN      
        1 ttataaatat ttgtaaaata actatcaatt ttttagttaa aagattggaa atgagtgggg
       61 gaggaattga agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat
      121 aggggagata gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat
      181 tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag
      241 cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa
      301 agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat
      361 caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat
      421 gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt
      481 taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc
      541 cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga
      601 taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta
      661 ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat
      721 taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg
      781 acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt
      841 ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa
      901 tttttcagta actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag
      961 gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa
     1021 tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa
     1081 tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt
     1141 gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt
     1201 aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga
     1261 acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga
     1321 agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa
     1381 cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat
     1441 cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt
     1501 attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga
     1561 caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga
     1621 atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta
     1681 tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc
     1741 aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac
     1801 agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga
     1861 ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac
     1921 tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa
     1981 cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa
     2041 aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca
     2101 aatatttgct catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc
     2161 tgtacctaaa cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa
     2221 atctagtttt gctgtaatca ttggtttatt gatagtaact atctgtgctg ggatattctt
     2281 acaaaaatat aaaaaagttt agttagaata taatattttt tgttaaatat gactgaaatc
     2341 aaaagaataa aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt
     2401 ttcgtagaat caataaatat ggatttggct aactatggaa gaatactcgt attagttttc
     2461 agcgttcata ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt
     2521 gctaaataaa gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa
     2581 ttttcataaa ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc
     2641 ataatcaacg tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata
     2701 gcagcatcga tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc
     2761 actgtacctg ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag
     2821 cgcttttctt ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag
     2881 atgacaagat agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga
     2941 ccaggtaggg aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga
     3001 aaagagatac ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc
     3061 gcgcatcacc ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata
     3121 atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag
     3181 gtgtttttag agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct
     3241 tggagtcgat tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt
     3301 cgttgctaga tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga
     3361 accacgtgat taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg
     3421 gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat
     3481 aatacgtcct cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat
     3541 ggagtatttg attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt
     3601 atttttattt accttgattg gctcattacc attagggatt gttttagcat taggattgtc
     3661 tttgcatttt aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac
     3721 accgctgttg ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt
     3781 tgatcgtttc gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga
     3841 gatctttcgt ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt
     3901 gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt
     3961 tttaccttct gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat
     4021 attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt
     4081 actgcctttg ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcgt
     4141 aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat
     4201 gattgaattg aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa
     4261 tctaatcatt ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac
     4321 cacgctttta cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga
     4381 cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt
     4441 ccaagacttc cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa
     4501 aatggtattg aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc
     4561 attagggatt agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca
     4621 gcgggtagcg attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc
     4681 aacaagtgct ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa
     4741 agaagatggg gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg
     4801 tgatcaatta ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa
     4861 accagttttc tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa
     4921 aaaacttcag cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta
     4981 gacgatacat ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat
     5041 attgatttag ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt
     5101 gactggtcaa tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc
     5161 tattccaaaa cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac
     5221 gatcaagtgc tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat
     5281 aagagattag gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa
     5341 gtattaaaag agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca
     5401 ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac
     5461 tattatttga aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag
     5521 agtgaggatt ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat
     5581 gaagccctag atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt
     5641 ggcgaagatg ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca
     5701 gtcccttttt ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg
     5761 gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttttcggt attaagccaa
     5821 atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact cgaaactaag
     5881 cgactttttc acaacctcct ttacataatt ttattttgtt gtggaagggg tttcgtgtta
     5941 ttctaaaaga ggaacgattg aaggttatag gaaaggaaga gatgaatgga acacaaagaa
     6001 cttacctcat ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa
     6061 ggagcttggc aagaagatgg gaaaggagaa tcagtctggg atcgatttgt acggatacct
     6121 ggaaagacat ttaaaggaac gaacggtgat ttggcagtag accactatca tcgttataaa
     6181 gaagatattg ctttaatgaa agaacaaggt ctgaaagctt accgcttctc tgtagcttgg
     6241 acacggattt ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc tttttatgag
     6301 agattgatcg acgaattgat tgaaaacgat attgaaccta tgttgacttt atatcattgg
     6361 gatcttccac aagcattgca agatgaatat aatggttggg aatcacgtca gatcattgaa
     6421 gatttcactc attatgcaga aacattattt gaggcattcc gcgggaaagt ccattactgg
     6481 attagtttga atgaacaaaa tatcttcact tcattaggtt atttgttagc agcacatccg
     6541 ccaggtgtga cagacccgaa acgaatgtat gaagttaacc atattgctaa tttagcaaat
     6601 gcatctgtca tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt
     6661 gcgtatacgc caaattatcc aattgacagt aatcctgaaa atgttctggc tgctgaaaat
     6721 gcagaagacc tgatggccaa ttattggatg gatgtctata tgtggggaaa atatccgatt
     6781 gctgccatga ggttcttaga agaaaaagga tgggcaccaa cgattgaagc tggcgatgca
     6841 gaattgctag aatctgctaa accagatttt cttgggatca attactatca aacggcgacg
     6901 aatgcattca atccattaaa cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa
     6961 agttcatcag aagaaactgg agttcctgga atgtataaaa aagttgagaa tccatttgtc
     7021 gaacgaacaa attgggactg ggagattgac ccagaaggat taaggatcgg actgcgtcgt
     7081 atcacaagcc gttaccgcat gccggtactg attacggaaa atggcttagg agagtatgac
     7141 aagttgacag aagataaaca aatccatgac gactatcgaa tcaattactt acaaagccac
     7201 gtcaaagcca tcaaagaagc aatttcggat ggagcagaag tcctaggcta ttgcacctgg
     7261 tcttatacag atttgcttag ctggctgaac ggttatcaaa agcgatatgg atttgtatat
     7321 gttgatcaag acgaaacaca agaaggatct ttggaacgat ataagaaaga cagttattac
     7381 tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata cttaaaacaa
     7441 gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga aattaagaaa
     7501 aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt
     7561 tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa
     7621 agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc
     7681 aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct
     7741 ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa
     7801 atacttacca aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga
     7861 atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg
     7921 gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata
     7981 tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata
     8041 acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac
     8101 agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta
     8161 atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat
     8221 tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga
     8281 aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaatatg
     8341 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     8401 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     8461 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     8521 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     8581 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     8641 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     8701 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     8761 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     8821 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     8881 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     8941 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     9001 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     9061 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     9121 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     9181 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     9241 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     9301 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     9361 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     9421 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     9481 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     9541 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     9601 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     9661 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     9721 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     9781 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     9841 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     9901 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     9961 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
    10021 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
    10081 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
    10141 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
    10201 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
    10261 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
    10321 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
    10381 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
    10441 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
    10501 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
    10561 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
    10621 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
    10681 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
    10741 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
    10801 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
    10861 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
    10921 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
    10981 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
    11041 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
    11101 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
    11161 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
    11221 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
    11281 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
    11341 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
    11401 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
    11461 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
    11521 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
    11581 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
    11641 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
    11701 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
    11761 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
    11821 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
    11881 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
    11941 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
    12001 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
    12061 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
    12121 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
    12181 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
    12241 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
    12301 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    12361 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    12421 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    12481 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    12541 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    12601 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    12661 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    12721 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    12781 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    12841 tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt
    12901 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
    12961 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
    13021 gatctttcct attttgaata aagtgtttct gtataatttt ttattttata agtaagataa
    13081 ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt
    13141 tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg
    13201 aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg agactgactt
    13261 ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac
    13321 gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc
    13381 aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga
    13441 accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa
    13501 tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc
    13561 tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg
    13621 aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg
    13681 acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa
    13741 aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact
    13801 tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt
    13861 ac
//
LOCUS       DAYYAJ010000077        13809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000077 DAYYAJ010000000
VERSION     DAYYAJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.077"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHATZ_001894"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHATZ_001894"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8404415.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHATZ_001895"
     CDS             complement(2262..2513)
                     /locus_tag="ACHATZ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8404416.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHATZ_001896"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHATZ_001896"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8404417.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHATZ_001897"
     CDS             3463..3681
                     /locus_tag="ACHATZ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404418.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACHATZ_001898"
     CDS             complement(3970..4482)
                     /locus_tag="ACHATZ_001898"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8404419.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHATZ_001899"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHATZ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8404420.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACHATZ_001900"
     CDS             complement(6935..7378)
                     /locus_tag="ACHATZ_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8404421.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACHATZ_001901"
     CDS             complement(7379..8167)
                     /locus_tag="ACHATZ_001901"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8404422.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHATZ_001902"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHATZ_001902"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8404423.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACHATZ_001903"
     CDS             complement(9282..9869)
                     /locus_tag="ACHATZ_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8404424.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHATZ_001904"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHATZ_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8404425.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="ACHATZ_001905"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="ACHATZ_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8404426.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="ACHATZ_001906"
     CDS             complement(12786..13721)
                     /locus_tag="ACHATZ_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8404427.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4470 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DAYYAJ010000078        13774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000078 DAYYAJ010000000
VERSION     DAYYAJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.078"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACHATZ_001907"
     CDS             complement(143..1345)
                     /locus_tag="ACHATZ_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8404428.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACHATZ_001908"
     CDS             complement(1348..1542)
                     /locus_tag="ACHATZ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8404429.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACHATZ_001909"
     CDS             complement(1589..2437)
                     /locus_tag="ACHATZ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8404430.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACHATZ_001910"
     CDS             complement(2642..2992)
                     /locus_tag="ACHATZ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404431.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACHATZ_001911"
     CDS             3170..4033
                     /locus_tag="ACHATZ_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8404432.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACHATZ_001912"
     CDS             4657..5649
                     /locus_tag="ACHATZ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404433.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACHATZ_001913"
     CDS             5665..6549
                     /locus_tag="ACHATZ_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404434.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACHATZ_001914"
     CDS             6598..8205
                     /locus_tag="ACHATZ_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8404435.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACHATZ_001915"
     CDS             8206..9690
                     /locus_tag="ACHATZ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8404436.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACHATZ_001916"
     CDS             9867..10859
                     /locus_tag="ACHATZ_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8404437.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACHATZ_001917"
     CDS             10911..11780
                     /locus_tag="ACHATZ_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8404438.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ACHATZ_001918"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ACHATZ_001918"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8404439.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4226 a         2328 c         2842 g         4378 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aata
//
LOCUS       DAYYAJ010000079        13707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000079 DAYYAJ010000000
VERSION     DAYYAJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.079"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..349
                     /gene="yaaA"
                     /locus_tag="ACHATZ_001919"
     CDS             107..349
                     /gene="yaaA"
                     /locus_tag="ACHATZ_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8404440.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            336..1460
                     /gene="recF"
                     /locus_tag="ACHATZ_001920"
     CDS             336..1460
                     /gene="recF"
                     /locus_tag="ACHATZ_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8404441.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1457..3403
                     /gene="gyrB"
                     /locus_tag="ACHATZ_001921"
     CDS             1457..3403
                     /gene="gyrB"
                     /locus_tag="ACHATZ_001921"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8404442.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3423..5894
                     /gene="gyrA"
                     /locus_tag="ACHATZ_001922"
     CDS             3423..5894
                     /gene="gyrA"
                     /locus_tag="ACHATZ_001922"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8404443.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6130..6429
                     /gene="rpsF"
                     /locus_tag="ACHATZ_001923"
     CDS             6130..6429
                     /gene="rpsF"
                     /locus_tag="ACHATZ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8404444.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6479..6991
                     /gene="ssb"
                     /locus_tag="ACHATZ_001924"
     CDS             6479..6991
                     /gene="ssb"
                     /locus_tag="ACHATZ_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8404445.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7017..7253
                     /gene="rpsR"
                     /locus_tag="ACHATZ_001925"
     CDS             7017..7253
                     /gene="rpsR"
                     /locus_tag="ACHATZ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8404446.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7396..9369
                     /locus_tag="ACHATZ_001926"
     CDS             7396..9369
                     /locus_tag="ACHATZ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8404447.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9375..9827
                     /gene="rplI"
                     /locus_tag="ACHATZ_001927"
     CDS             9375..9827
                     /gene="rplI"
                     /locus_tag="ACHATZ_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8404448.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10101..11468
                     /gene="dnaB"
                     /locus_tag="ACHATZ_001928"
     CDS             10101..11468
                     /gene="dnaB"
                     /locus_tag="ACHATZ_001928"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8404449.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12248..13540
                     /locus_tag="ACHATZ_001929"
     CDS             12248..13540
                     /locus_tag="ACHATZ_001929"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8404450.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4575 a         2471 c         3016 g         3645 t
ORIGIN      
        1 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
       61 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
      121 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
      181 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
      241 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
      301 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
      361 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
      421 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
      481 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
      541 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
      601 aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg
      661 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
      721 agatcaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
      781 atcttgtcca ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg
      841 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
      901 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
      961 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
     1021 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
     1081 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
     1141 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
     1201 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg
     1261 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc
     1321 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
     1381 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
     1441 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
     1501 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
     1561 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
     1621 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
     1681 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
     1741 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
     1801 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtaa acgcgctttc
     1861 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
     1921 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
     1981 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
     2041 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
     2101 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga tcaaaagtta
     2161 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
     2221 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
     2281 tatcatgagt tttgccaaca atatccatac gtatgaaggt ggaacacatg aatcaggatt
     2341 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
     2401 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
     2461 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
     2521 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
     2581 aagtgtgggc cggcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
     2641 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
     2701 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
     2761 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
     2821 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
     2881 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
     2941 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
     3001 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
     3061 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
     3121 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
     3181 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
     3241 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
     3301 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
     3361 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
     3421 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
     3481 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
     3541 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
     3601 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
     3661 ctcatggaga cattgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
     3721 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
     3781 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
     3841 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
     3901 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
     3961 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
     4021 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
     4081 caggtggttt ggttatgggg aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
     4141 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
     4201 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
     4261 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
     4321 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atccgttatc ttgaataact
     4381 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
     4441 gtgtgccgaa agttttgagc ttgaaacaaa tccttgaaaa ctatatcgaa catcaacgcg
     4501 aagtcatcac acgacggacg gtctttgaaa aagaaaaagc agaagcaaga gcacacatct
     4561 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
     4621 ctaaatcaga tgacgaggca aaagcaacaa tgatcgaacg gttcgattta tccgatcgcc
     4681 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
     4741 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
     4801 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
     4861 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
     4921 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
     4981 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
     5041 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
     5101 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgta
     5161 cagccaaagg aatcccaatc atcaatttac taggtatcga ttcgagtgaa agcatccagg
     5221 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
     5281 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
     5341 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
     5401 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
     5461 gcgatatggg acgtacagct tccggggtca gagggatacg cctgcgtgag gaagattatg
     5521 tcgttggggc agccttaatg aaggaaaatc aagaagtatt agtgatcaca gaaaaaggtt
     5581 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
     5641 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
     5701 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
     5761 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
     5821 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
     5881 gatcagaaga ataaccatca agactaagtg tgtaagctag caagaataac cgattaaaaa
     5941 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
     6001 gttcattctg tattgctttt gaccaaaata gagtgtataa ttagagcttg tgagtagtct
     6061 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
     6121 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
     6181 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
     6241 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
     6301 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
     6361 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
     6421 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
     6481 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
     6541 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
     6601 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
     6661 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
     6721 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
     6781 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
     6841 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
     6901 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
     6961 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
     7021 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
     7081 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
     7141 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
     7201 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
     7261 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
     7321 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
     7381 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
     7441 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
     7501 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
     7561 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
     7621 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
     7681 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
     7741 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
     7801 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
     7861 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
     7921 ttatctattg ataactatgc agatgccatt gataaattag atgacaaaga gatttcttat
     7981 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
     8041 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
     8101 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
     8161 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
     8221 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
     8281 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
     8341 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
     8401 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
     8461 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caatgagaac
     8521 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
     8581 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
     8641 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
     8701 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
     8761 acttatattg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
     8821 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
     8881 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
     8941 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
     9001 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
     9061 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
     9121 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
     9181 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
     9241 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
     9301 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
     9361 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
     9421 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
     9481 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
     9541 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
     9601 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
     9661 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
     9721 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
     9781 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
     9841 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
     9901 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
     9961 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    10021 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    10081 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    10141 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    10201 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    10261 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    10321 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    10381 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    10441 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    10501 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    10561 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    10621 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    10681 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    10741 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    10801 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    10861 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    10921 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    10981 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    11041 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    11101 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    11161 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    11221 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    11281 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    11341 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    11401 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    11461 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    11521 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    11581 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    11641 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    11701 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    11761 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    11821 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    11881 atctgcggta atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt
    11941 cttattactt gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta
    12001 gcttcttcga aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc
    12061 cttcttattt cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa
    12121 aaagataaag ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt
    12181 atttgaaata tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg
    12241 tgttcacatg tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa
    12301 aatcacagac tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa
    12361 tgcagggcac acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg
    12421 gattttttat aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc
    12481 attggttaaa gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat
    12541 ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga
    12601 tgctaagggc gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga
    12661 taaagcagct cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga
    12721 acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga
    12781 accacttgat tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca
    12841 atacgtcaca gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt
    12901 gtttgaaggc gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac
    12961 ttcttctaac ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat
    13021 caataaagtt gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc
    13081 aactgaatta tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac
    13141 gacaactggt cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa
    13201 acgagtttca ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga
    13261 aacagtaaaa atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc
    13321 aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga
    13381 agacattaca ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg
    13441 tcgtatctca gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac
    13501 acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa
    13561 tcaggacgtt cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa
    13621 gaggtagtga aagagacgtt tagccttatt gccgataggc tgtctcttga acactgttta
    13681 ttcagaataa gggactgtga caaaaac
//
LOCUS       DAYYAJ010000080        13603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000080 DAYYAJ010000000
VERSION     DAYYAJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.080"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..1072)
                     /locus_tag="ACHATZ_001930"
     CDS             complement(125..1072)
                     /locus_tag="ACHATZ_001930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8404451.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1262..1531
                     /gene="rpsN"
                     /locus_tag="ACHATZ_001931"
     CDS             1262..1531
                     /gene="rpsN"
                     /locus_tag="ACHATZ_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8404452.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1707..2069)
                     /locus_tag="ACHATZ_001932"
     CDS             complement(1707..2069)
                     /locus_tag="ACHATZ_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8404453.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2080..3201)
                     /gene="alr"
                     /locus_tag="ACHATZ_001933"
     CDS             complement(2080..3201)
                     /gene="alr"
                     /locus_tag="ACHATZ_001933"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8404454.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3217..3567)
                     /gene="acpS"
                     /locus_tag="ACHATZ_001934"
     CDS             complement(3217..3567)
                     /gene="acpS"
                     /locus_tag="ACHATZ_001934"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8404455.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3697..5208)
                     /locus_tag="ACHATZ_001935"
     CDS             complement(3697..5208)
                     /locus_tag="ACHATZ_001935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8404456.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5530..6882)
                     /gene="murF"
                     /locus_tag="ACHATZ_001936"
     CDS             complement(5530..6882)
                     /gene="murF"
                     /locus_tag="ACHATZ_001936"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8404457.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7035..8111)
                     /locus_tag="ACHATZ_001937"
     CDS             complement(7035..8111)
                     /locus_tag="ACHATZ_001937"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8404458.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8307..10052
                     /locus_tag="ACHATZ_001938"
     CDS             8307..10052
                     /locus_tag="ACHATZ_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8404459.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10332..10769)
                     /locus_tag="ACHATZ_001939"
     CDS             complement(10332..10769)
                     /locus_tag="ACHATZ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8404460.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10753..12126)
                     /locus_tag="ACHATZ_001940"
     CDS             complement(10753..12126)
                     /locus_tag="ACHATZ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404461.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12448..12936)
                     /locus_tag="ACHATZ_001941"
     CDS             complement(12448..12936)
                     /locus_tag="ACHATZ_001941"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404462.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12958..13560)
                     /locus_tag="ACHATZ_001942"
     CDS             complement(12958..13560)
                     /locus_tag="ACHATZ_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8404463.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3897 a         2947 c         2464 g         4295 t
ORIGIN      
        1 gggaaagata attttatgcc aaagttttta tctattatac gtatacgcaa aaaagctaaa
       61 acattttttg tctttgcccc tacaaaaatg tttcagcttt tttcgttttt tattcctgta
      121 tttcttattt ttcgaatgac aggatgattt tcccagcagg aggatgtcct tcaactagct
      181 gatgtgcttg gatgacatgc ttaagtgaag cagacagatt cattgaaata aaaacatgaa
      241 aggctccttt ttggtaggct ttgattatgc cagcaaaggc ttctgcatct agatattcct
      301 ttcttggaac aaacgattca taaaagcctt cctttttttg ccgcaaatct agatctggga
      361 ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat ctggatgccg gtttctcctt
      421 tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg atcagcaaat tgcagacctg
      481 gatttgtttt atcataagca gcaaatgctg aagctcctaa tttttttacc ttttcctcat
      541 ttttactgga tgcactggtt aagattcgga ttcccttttc atggagaagc tggatcagac
      601 ttgacccgac cgcacctgat gctcctagta tcattacagt atctgtcggc tggatttccg
      661 tcaaatggtt gattagatta taagccgtaa ttcctggcgt gaccattccg gctgcttctt
      721 cccaactcat tttatctgga atttttgcta cttttgctga tggcaacacg acttcttctc
      781 cgtaagtccc gccgactgca tggacagcta ctcgatcacc tacatgaaca gttgtgacat
      841 ctgaaccaat ctctgtcaca ataccagcgc catcattacc tggtacataa gggaatttca
      901 atgttcgtat ctctttcatt tctcctaaac gcaaggcaac atcataagga ttcacactga
      961 atgcttttat ttctacacga acatgtccag aaccgacttc tctgggatga gcatcgattt
     1021 cttcaaaaac atcttgtgct tctccgtaac cgttaattgc aaatcttcgc atggtcacca
     1081 tctccctttt tctttaagtt tacgtttatg cctttatttt gacaaaaaaa atgtcttttc
     1141 gttttggcgc atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt gttaaattat
     1201 ttatctaaaa gcgtaaagat tacgatttac gctaataaaa aataattgga ggaatttgtt
     1261 gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta
     1321 tgctgcttta cgccaggaat taaaagcgca aaaagattac gaaggcttgc gtaaacttcc
     1381 tttagatgct cgtccagaac gattgaaaaa tcgtgattta atcgatggac gccctcgcgg
     1441 atacatgcgt aaattcggga tgtcccgtat aaaattccgt gaattagcac ataaaggttt
     1501 gattccagga gtaaaaaaag ccagctggta atccaagtct ggctgtaatg taaaggagga
     1561 gggcgcatgg accactcctt ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa
     1621 tgctggtact agaagcagac gtatcttttt ttacgcacag ctggtccttt tttatatgta
     1681 aaggctgcat ggttacttcg ctatatttat ttagtaacag gagtcagtgc ttcgataccc
     1741 acactaattt tcaatgctcg gtcaacttct gccataatgg gtcgatccaa atggcagacc
     1801 ttttctttca atcttgattt atcgatcgtt ctgatctgct ccaacaggat aacggagtct
     1861 ctttctattc ctgttccagt agctttgatc ccaatgtgag tcggaagctt aggtttagcc
     1921 atttttgccg ttatggccgc aacaatgatc gtcgggctaa aatggttacc tagattattt
     1981 tgaataacca aaactgggcg tgttccgcct tgctccgatc ccacgactgg agaaagatca
     2041 gcaaaataaa tatctcctct ttttaccata ggatcctcct tattcttgat attctcttgg
     2101 tatccgctgt cctaatccgc aagccacttc atagtggatc gtacctaatt gatctgctat
     2161 atcctgcata gtgatttctt tgtctgcatt tttgccgata agcgtcactt ttgttccgac
     2221 aggaaattct tgaggcaatc ggatcatcag ttgatccata catacacgcc caatcgtttc
     2281 acagtagttc ccttctacca atagagaaaa accctgcatc tttcgtggcc agccatctgc
     2341 ataaccgatc ggtatcgttc cgatccactc tgcttcagga gtgatatatg tttctccata
     2401 gccgattcct tcacctgctg ggagtttttt gacttgaatc aattcagaaa ccagctcaag
     2461 tgctggctgc aatggataaa cttccggtag tgcgtgccca gagggattaa gtccgtacat
     2521 tgctactcca taacgaatca tattgccgat tgtttcatca tgccataaag ccgttgcact
     2581 attgctgaca tgtacatatc gaggaagttc gggaagtttt ttcagcactt ctataaagcg
     2641 ttctttttgc agattccaat atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa
     2701 taaaccttcc cattctaata cacgactttc tttgatgaat gcagctgcct cttttacttc
     2761 ttcaggtgta caaaatccga tacgacccat cccagtgtct gcttttagat gaatcgaaag
     2821 aggcgtctct agcttacttt tctctaaaac agcagctgct gcttgcaacc attcccgtgt
     2881 acctgcagtt actgataaat catgagcaac agctacagtc agtgcgtcgt aaggaaccat
     2941 attcaagata aggatgggct gagtaaatcc agcttctctc aactcgactc cttcatctaa
     3001 atttgaaaca caaaagcccg aagctccacc tgcaactgct gcctcagctg tttcaatagc
     3061 tccatgaccg tatccgttcg cttttactac tgcaaacaat tcttttcctt gcggaagtct
     3121 tgcaacttca tttgctacat tttcagttat agctttttta tgtatcactg ctttcgttgg
     3181 gcggtgccaa gcgacaacca tcgtttttcc ttctttctag tcttccaaaa taatttgtgc
     3241 aaaagctgtt tcatttgtat gagtaatact gacccataca ttgccttcat gaggtgaacg
     3301 cgtcacaaca ggctgaccat tttcattttt taaaatctcg acatcttgaa aggtaacttt
     3361 tccgatccca gttccccaag ctttagagaa agcctctttg caagcgtaac gcccgcctaa
     3421 aaattctact tggcgatgaa aaggcaagga ctggaatagt ttcatttcat cagaggtcaa
     3481 gattcgtgca atgaattttg gtttttcttc gatcagccga gtgatcctag gaagttcgac
     3541 tgcatcgatc ccaattcctt ttatcatatg cttttctctt ttcaataaat ttagaacact
     3601 ctcattctac caaattataa ccattttgcc acaaataagt ccacaaataa caaaaatgaa
     3661 agtttctctt aaaaaagaga aactttcatt ttatgtttaa tcgttatttg aacggattac
     3721 aaatccgcgt tttttatcat tgtgacgttt accgccatct ttttggctgt tattattttt
     3781 gctataaccg ccttttgatt tgtttccacg ataattaccg ccgtcacgac gattacggtt
     3841 gtttccgcca ccacgtttgc catttttatt gtatcctttt ttctgcattg gcaatgggcg
     3901 ttctggtgtg atcttcactg gcactgcatc agctggatct tttgctgtcg tttttagcaa
     3961 aagtgcaact aaatcttgtg cagaatattc ttctaataat ttatctgctg tttgcaggta
     4021 tttatctaga ccattttctg caagttttgt ttcgatttgt tcaactgcag cacctaattg
     4081 acctttgaat gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa
     4141 gttttcaatc acatgcaagt agtccatttc atttggtgta acgaaagtca cagacatacc
     4201 gccttttccg gcacggcctg tacgtccgat acggtgaacg tagctttctg gatcttgagg
     4261 gatatcatag ttgtacacat gagtgacacc tgaaatatct aatccacgtg cagcaacgtc
     4321 tgtcgctaca aggatatcta aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt
     4381 ttgttgagac aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg
     4441 tgccaattcg tctacacggc gttttgtacg tccgaagacg attgttaatt caggtgtttg
     4501 aacatcaaac aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc ggacatagta
     4561 ttgatcgatc aagtcagctg tcatttcttt cgctttgatc ttcacatgat gtggattttt
     4621 catgaattta acgccaatat tcttgatagc tggcggcatc gttgctgaga aaagtaatgt
     4681 ttgtctttga tcaggaactt gtgaaatgat tttttcgata tcttccaaga agcccatatt
     4741 caacatttca tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg
     4801 gcggttaatg tggtcaagca tacgtcctgg tgtacccaca acgatatgtg gacgatcttt
     4861 caagccacgg atctgacggc caatatctgc tccgccgtaa acggcttgta cacgtacttt
     4921 tttgtcgcgc cctaaacgat ataattcttc ttgtgtttgg atagctaatt cacgtgttgg
     4981 tgcgataact aaaccttgta attcgtgacg atctggatcg attttttcta acattggtaa
     5041 tccaaatgca gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc
     5101 taatgctagt ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc
     5161 gactgatttc aacaattctg gtgataattc tagttcttta aatttcaaat gaatcctcct
     5221 atatggttgt taactgagga tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag
     5281 tgtattgttt ctttttgaca gtggccacgc acgtttttat ccattcttag cccctgtggc
     5341 accaccgaaa aatcactttg cgaagtttag gcattagaaa agccaaacgg gtcgcgttca
     5401 gctttatcat tattatgaca tctggcttca acaagttgtt tctatggcaa aaccagtcca
     5461 atttctcttc atctttcggc aataggttat tctaacatag tttttcgttt tctgcaagtt
     5521 tacagatttt tatttcattt gctgaagttg ttctaccact tcaaataagc ccatgctgtt
     5581 gctgcctttt agcataatac tatctgttgc ttcaagattt tgtttgatag catcaatcaa
     5641 tttattcttt tctttttcaa aatagaacac aggtagttgg gggtattttt ggttcaattc
     5701 ttcttttagt gcatgcattt gttccccata aaggaaaatc atggcgaatt tctcaggatc
     5761 gatatgttca ctcatttgcg catgcatatt taaagactct ttccctaatt ccagcatatc
     5821 tgctaacaca gcgattctgc gtccttttgt tggcagattt ttaaaagtat ctaataccag
     5881 tcccatggct gttggatttg cattgtagac atcgctcaaa atagctgcac catttcctgc
     5941 cgttagccat tcggttctgt tttgagtgat agaaacatgg gcgagccctt ggcgtatctc
     6001 aggtatggac agtccaaaga agcgcccgat cgtatacgca atcaaagcat tcgttacatt
     6061 ataacttcca ggcagcggga tcgtaaatgt ttctccttct actacaaaat cggtttgtgc
     6121 ttttcctttt gctgtcactt ccgcatgaac atcgccattt ccaataccga atgttgtgac
     6181 tgtttgtact aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag
     6241 taatccgtct gatacaagtc cgtcagtgat ttccattttg gctttagcga ttccttctcg
     6301 cgacccaaga ttttctatgt gtgcttcacc gatcattgtg attgctgcag cttttggttg
     6361 agccataagt gaaagttccg tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat
     6421 cttttctgta tcttggggca tgtgcaaaat cgtgtaagga agaccaatat tattgttgta
     6481 atttccttgc gtcttatatg ttttgtattt ttcagacaag acagacgcgg ttagatcttt
     6541 cgtggtggtt ttaccattgc ttcctgtaat tgcaacgact tctgcgccta ctttttccaa
     6601 gtaataggtt gccaattgct gcatggcttt caaaggatct gctacaggaa taactggaaa
     6661 attttttgga gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat
     6721 cgcttgctgg ataaattcat gtccatctcg cgtaccagca agaggaacga ataaacttcc
     6781 ttcggttatc ttccggctgt caaattcgac attggtaatc tttgtcagtg cagagcattc
     6841 gctgcccacg gcttgtgcaa tttcttgtat tgttagattc attgagatct ctcctaaaaa
     6901 aggttgtgaa aaaagcgttt ggcctaaacg gcgggttcgc atgactctca cttccaagta
     6961 aatttctcta ttcgtattta gaagctagaa tgaacgcggt tacgcaatca ctccagcctc
     7021 ttctttattt ctctttattc atttttttca aaaaaagatt gacgctgatg gtatcgattc
     7081 attcctaact ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat attttcccat
     7141 aagagtgggt acatactgaa ctccgtaaat cctggcatag agtttaattc attcaggaat
     7201 aattcatttt tatttgtcaa aaagaaatcg caccggctca atccgcttcc acctaacatc
     7261 gtgtaagcta acttcgcgta ctcttgcgct ttttgataaa cttcttctgg cacttcggct
     7321 ggaatctgca tttcgatttt attattgata tattttgctt cataatcata gaatgctacg
     7381 tcttttacga cttcaccagg caaagtcgtc cgaacgtctt catttcctaa tacagcaact
     7441 tcgatttcgc gcgcttcaat tccttgttca acgattgctc gagaatcata ctgataggct
     7501 gttgctaaag cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa
     7561 cccatattcg ccggtttgac aaacatcgga taaagcaaag aaccttcaca ttgatcaaat
     7621 acttttttag gattttcttt ccattgattc ttaagtactg gtacataagg aacttgcggc
     7681 acaccagcag cttgtaaaat atacttggtc atgattttat ccatggcaca tgcactggtc
     7741 aatacgcctg cgccgacata aggcatattc aatgtctcta agaagccttg gatcgttcca
     7801 tcttccccgt ttggcccatg taaaactgga aaaacgatgg ctccttcttc tttgatttcg
     7861 cccggattga tcacttttcc tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa
     7921 agatgcaaga catctttgct ggcaggtttt tctgttagta atggaccttt gacccattgt
     7981 ccttcttttg taataaatac gagttgaact tggtaataat tataataaat ggcatttaaa
     8041 actgaaaatg cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa
     8101 gtaatcttca aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt
     8161 ttagcacaaa acaaaaataa acgaataact tatttataaa aaaacgagcc agcttctaaa
     8221 agaacgagcg tcatttattt tttgtcagct agattatttg tcccgttttt tataaaaagt
     8281 tatgataaag aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag
     8341 aaatcattcg agtgttcatc aattacaatg tagtgccaaa ctttgtacag caaaaaaatc
     8401 ccgaacaggt aaaaaaagcc tttgaagaac taggccccac cttcatcaag ataggacaga
     8461 tgctttctgt tcgagaagat ttgctttctt ctgccttcac tcaaacattc aaaacattac
     8521 aagatagtgt acctagcgac acattttcaa cggtcaaaaa gacgatcgaa acagaacttt
     8581 ctctttcatt atcagatata ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta
     8641 tggggcaggc tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt aagatccaac
     8701 accctaacat cgccgaagag atccgacttg atcttcaact atttgaacga gcgatccctt
     8761 tgatcaaata cattcctgaa actagcgtag ttgatctaaa aggggtactc caagaagtaa
     8821 aacgttcctt gatcaatgaa atggacttct taaaagaaag tcaaaatggc gaacaattct
     8881 atcaaaagaa caatggctgg aaagagatcc gcagccccaa gatttatgac gctttttgtt
     8941 cgaaaaaagt gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg
     9001 ccgaaaacaa aactgaaact tttatcactg gaattcaaaa caagcagctg aaacaagaag
     9061 ttgcaaagct tttagttgag aactttatga aacaggtgtt tgatgatggt tttttccatg
     9121 ctgatcctca tccagggaat ctgctattcc atgtactaac aaaagaagaa cagacacaag
     9181 ctagccgaaa aactgaaaca gtccatgaaa aagagttcgg ttcctttgct tttcgagcct
     9241 ccacttctgc agaagaccct gttgcgccat ataccatcaa ctacatcgat tttggtatga
     9301 tggggcatct ttctgcagga cttaggcaaa aactcacaca agccgtttta gcgctgtata
     9361 cgaaagatgc ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt
     9421 ttgatgaaag tcggttccac caagaactga caagttttct tgaacagtat tatgattctc
     9481 ctattgatga aatcaacttg caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa
     9541 ataatctgca gttcgatcgt gatatcacgc ttttgctgaa agcatttggg acattagaag
     9601 gggtgatccg cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg ccttttgctc
     9661 aacattattt tctcactcat ctggatgtag aagatacatt gaaacaaagc ggattagact
     9721 tattggaagg gatgaaggct gcgcctaaga tccctcaaca gctccaccat ctattagaaa
     9781 tgtggacttc gggacaagga aaagtcaatt tggaattgaa aaagcaagac aaattattga
     9841 gcaggatcga aagtatgatc aatcggcttg ttttcggtat gattttagct gcattgatcg
     9901 tagggtcctc cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc gtttccttat
     9961 tagggatatt tacgtatgct attgccgcat tcgtcattat ttttttggca atcgatgcat
    10021 tgatccaaat gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct
    10081 tcaagaaata agaaggggtt ttacataaat tgtttctcaa atttttgtga aaattcggct
    10141 tatttttcga agaggctact tctgaaacac cgagaataaa aaatgtgaaa aagctgttct
    10201 gcacccagta ataagaacaa tcaaaaaggg aacatggaac acatttatcc tgtttccctg
    10261 ttcccttttt gatgtattcg agcataaata aaataccttc gttacctact atcttctgta
    10321 cttggtaaac cttagtcaat tgcctttttt aacgcttgac gaaaagacat atgatctttc
    10381 tccgtaaagg gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga
    10441 taacgttgtt ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg
    10501 aacactcgca cacccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa
    10561 aagtcattct tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct
    10621 acgtagacga tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc
    10681 acgataacca cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga
    10741 gaaccatctc catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac
    10801 agttactaat ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg
    10861 tacactttct atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc
    10921 tcccatttct tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact
    10981 ccctttattt tgagtagtat acttgacaac gatccggcga tccattggaa tagctgtatc
    11041 agtttgataa tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag
    11101 aaagacacct atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc
    11161 aagcaattgc atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat
    11221 ttttgctgca atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa
    11281 tccgtacagt atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc
    11341 atttagaaaa gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc
    11401 taaaacaaac acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag
    11461 cctttcatcg aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa
    11521 aaataaaaca agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc
    11581 gtaacgcgga taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt
    11641 aaagcttgct gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag
    11701 ccagatagct gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc
    11761 gtgaaaattg accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac
    11821 acttgaaaaa taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc
    11881 accaccaatc agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact
    11941 gttgactaaa aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt
    12001 cagccaatta tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt
    12061 gttacttaag ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc
    12121 gaacatgaca atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt
    12181 aaaggggaaa taaaccaatt ttacagaata tattcttgta aaaaaaaata cagaaagaag
    12241 cataagcttc tttctgcatt tttacggtac ttgattactc aagcagacaa gcccttctcc
    12301 acaaaagtct tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa atcaattatc
    12361 ctgtgcctcc aaaatcaatt aggagctctg taaaccagag tccttctcga cccgtcgcat
    12421 aaatttactt taacacagtt cctatgttta tgcgagagct tttttcattt tataatcgac
    12481 catttgaaat ccttgaccga tatctgaaat aagttcttct gtaatctcaa atccttcatg
    12541 cttataaacc tcgattgctt gatgattccc ctgattcact cgcaaaaaga tctctctttc
    12601 ctttgtttgc gcagcaagtt cttcatacca gcgaaaaact gaagaagtca atccttgacc
    12661 acgtacttta tttagcaaat ataatttact taaataaatt tttccattta tctcttcata
    12721 agcagtgtaa ccgacagtct tttcttcgct ttttaataaa aagtaggaca caccttctgt
    12781 cagttctttt tgaatctgtt tttttgactg ataagttttc agcatgtaat ccacttgttt
    12841 ttgaccaatg atcggcacaa aaacttccgg ccatacttct tgtatgactg ccatcagctc
    12901 atccagttca ttttctgtta cttttatttt ttccatacac ccacgtcctt ttttacatta
    12961 taaatgacct tgcgcttgat ggaataaaaa gagcatgatc atgctataca cacccaccga
    13021 ccacggtttc aagaaaatca caatctgcat aaattcccta aaagataatt tcgtcaaacc
    13081 agctactaga cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa caactagtac
    13141 ttttttaata tctttcgttt ttcttgtcag caattcatca aatttctgat acttttttgg
    13201 cgataaaata agctggacaa aggctacgcc atagtaacgg acaagcaaaa atccgattgc
    13261 ttccccaatc actaggccaa tacaactata taaaagccca aatccattgc caaacatcag
    13321 catgcccgct actgaggaga tccctcccgg caaaatagga atgatcactt ggatcacttg
    13381 caacagaaca aatatcatca ctgcatactt gccaaattgc tggataaacg cttgcaaaga
    13441 cgtgctcgat cgaaaaatcc catgatgata agcataaaga atcaacacaa agaaaaaaaa
    13501 cagaccaata atgggcatga tctgaagaca tttttgtaaa actgatcctt ttttagacat
    13561 ctatattccc cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DAYYAJ010000081        13325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000081 DAYYAJ010000000
VERSION     DAYYAJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.081"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHATZ_001943"
     CDS             5..2377
                     /locus_tag="ACHATZ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8404464.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHATZ_001944"
     CDS             2438..3898
                     /locus_tag="ACHATZ_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8404465.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHATZ_001945"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHATZ_001945"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8404466.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHATZ_001946"
     CDS             6129..6278
                     /locus_tag="ACHATZ_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404467.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHATZ_001947"
     CDS             6353..6946
                     /locus_tag="ACHATZ_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8404468.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHATZ_001948"
     CDS             6959..7858
                     /locus_tag="ACHATZ_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8404469.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHATZ_001949"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHATZ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8404470.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHATZ_001950"
     CDS             8724..8984
                     /locus_tag="ACHATZ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404471.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHATZ_001951"
     CDS             9429..9635
                     /locus_tag="ACHATZ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8404472.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHATZ_001952"
     CDS             9656..9886
                     /locus_tag="ACHATZ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404473.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHATZ_001953"
     CDS             9902..10339
                     /locus_tag="ACHATZ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404474.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHATZ_001954"
     CDS             10332..11039
                     /locus_tag="ACHATZ_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404475.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHATZ_001955"
     CDS             11203..11403
                     /locus_tag="ACHATZ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404476.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHATZ_001956"
     CDS             11419..11598
                     /locus_tag="ACHATZ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404477.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHATZ_001957"
     CDS             11923..12186
                     /locus_tag="ACHATZ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8404478.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHATZ_001958"
     CDS             12180..12530
                     /locus_tag="ACHATZ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8404479.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHATZ_001959"
     CDS             complement(12554..13168)
                     /locus_tag="ACHATZ_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8404480.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4927 a         2201 c         2739 g         3458 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaa
//
LOCUS       DAYYAJ010000082        13234 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000082 DAYYAJ010000000
VERSION     DAYYAJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.082"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..2433
                     /gene="leuS"
                     /locus_tag="ACHATZ_001960"
     CDS             19..2433
                     /gene="leuS"
                     /locus_tag="ACHATZ_001960"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8404481.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2781..4334
                     /locus_tag="ACHATZ_001961"
     CDS             2781..4334
                     /locus_tag="ACHATZ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8404482.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4715..6499)
                     /locus_tag="ACHATZ_001962"
     CDS             complement(4715..6499)
                     /locus_tag="ACHATZ_001962"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404483.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6514..8235)
                     /locus_tag="ACHATZ_001963"
     CDS             complement(6514..8235)
                     /locus_tag="ACHATZ_001963"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404484.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8335..9096
                     /locus_tag="ACHATZ_001964"
     CDS             8335..9096
                     /locus_tag="ACHATZ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8404485.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            10063..10331
                     /locus_tag="ACHATZ_001965"
                     /pseudo
     CDS             10063..10331
                     /locus_tag="ACHATZ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            11003..>13234
                     /locus_tag="ACHATZ_001966"
     CDS             11003..>13234
                     /locus_tag="ACHATZ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8404486.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         4351 a         2270 c         2714 g         3899 t
ORIGIN      
        1 agaacaggag gacagcaaat gagttacaat cataaagaaa ttgagaaaaa atggcaaaaa
       61 tactgggcaa aaacgaatac tttcaacacc catgatgatc ctgaaaagcc aaaattttac
      121 gcactggata tgtttcccta tccatccggt caagggctac atgtagggca tcctgaaggt
      181 tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc aaggatacaa cgtgctgcat
      241 ccaatggggt gggatgcatt cggtctgcct gctgaacaat atgcgctaga tacaggaaac
      301 gatccagctg agtttaccca aaaaaatatc gaaacattcc gacgccaaat caattcttta
      361 ggatttagtt atgactggaa tcgcgaagtg aatacaactg atccggagta ttataaatgg
      421 acacaatgga tctttacgaa gctttatgaa aaaggtctag cttatgaagc agaagtagcg
      481 gtaaactggg tacctgaatt aggaacagtt atttcaaatg aagaagtgat cgatgggaaa
      541 agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc gccaatggat gctgaaaatc
      601 actgcttatg ctgatcgctt attggatgat ctagaattag ttgattggcc agaaagcatt
      661 aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag caaatattga atttaaagtg
      721 gctggtacgg ataaatccta taccgtattt acgacacgac cagatactct attcggtgca
      781 acttactcgg ttcttgcacc tgaactagat cttgttagag aaattacgac ccctgaacaa
      841 aaagctgctg tagaagctta tatcgaagag acagctaaaa aatcagatct aaagagaacg
      901 gatcttgcaa aagaaaaaac aggagtattt actggtgctt atgcaatcaa tccagttaat
      961 ggcaaggaaa tcccaatctg gatcgccgat tatgtattat cttcatacgg cacaggagcc
     1021 atcatggctg ttccagcaca tgatgaacgt gactttgaat tcgctcaaac tttcggacta
     1081 gaaattcttc cagttatcga aggtggagat gttgaaaaag ctgcttacac agaagatggt
     1141 acgcacatca actctgaatt tctagacggc atgaacaaac aagaagcaat cgataaaatg
     1201 aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag tcagctatcg cttacgtgac
     1261 tggctattct cccgtcaacg ttactggggt gaaccaatcc caatcatcca ttgggaggac
     1321 ggaacagtca cagctgtccc agaagaagag ctaccattac ggttgcctaa gacaaaaaac
     1381 atcaagccaa gcggtactgg agaatctcca ttagcaaata tcgaggagtg ggtcaatgtc
     1441 gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga atacaatgcc acaatgggca
     1501 ggaagctcat ggtattacct gcgttatatc gaccctcata acaaaaaaga actagcagat
     1561 tatgaaaagt tgaagcgttg gctaccagtt gacatctaca ttggtggagc agaacatgcg
     1621 gtacttcatt tactatatgc acgattctgg cataaattct tatacgatat cggtgttgtt
     1681 cctacaaaag aaccattcca aaaactatac aatcaaggaa tgatcttagg cgaaaacaac
     1741 gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg atgatgtggt cgaaacttac
     1801 ggtgcagata cactgcgcat gtacgaaatg ttcatggggc cattagatgc ttcgattgct
     1861 tggagcgaaa atggcttaga aggaagccgt aaattcttgg atcgtgtatg gcgtttgatc
     1921 gttgatgaga acaataaaat gcgtgaccgt atcactacat tgaatgatgg caagcttgat
     1981 aaagtgtatc atcaaacagt gaaaaaagtg acagaagact atgaaaatct tcatttcaat
     2041 acagcaatct cccaattgat ggtctttatc aatgaagcct ataaagtgga cgctctgcct
     2101 tatgaatata tcgaaggatt tgttcaacta ctcgcaccga ttgctcctca catgggtgaa
     2161 gaactatggg caatccttgg aaatgatggt ggtatttctt acgcaccatg gccaacatac
     2221 gatgaagcag cacttgttga agacgaagta gaagttgttt tccaagtaaa cggtaaagtt
     2281 cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg agctagaaaa agcagcatta
     2341 gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag tcagaaaagt tattgcggta
     2401 ccaaacaagt tagtcaatat cgttgctaat taattggctc tttgtcaata aggactgatg
     2461 agttgtgcaa aatcaaatct gggccagaat gaacttcatt ctgtctcaga ttttttgtta
     2521 tttcaaattg attaattgat tgatcaaaaa ggttttgatt ttcatgttct caaacacgtt
     2581 caatgtcttt ttcttctgaa tggtcttgta agagatctct gtaatcatca aatttttgtc
     2641 tattaatctt agtatttttt gatgtataaa ctacctgaaa ttgagagtac ataccaaatt
     2701 cataaatttt ttattagtgg gactagtgat tgtatggatt ttgacgcata atgaatgaag
     2761 tatgagaaaa ggagagacaa atggatcaag aactatttat tatcgaagta ttatcagcta
     2821 aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag aataatgttc aagcttttaa
     2881 gcttagtatt tttctctggt ttgctttttt tcggagctgt atcgttccta tatcgccata
     2941 tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt aaataatttg tatcactttt
     3001 cgtggggagt tggcggaata acaattgttg tgcttattgt ttttcttcta cttatttggc
     3061 tccgttacca tcaattgtcg cgtcatttcg atcaatggct tagtcaaaga gcacaacagt
     3121 ttttacataa taaaccaact tctcaagatg atgactatct ttattttgag aagacttcga
     3181 cgagtcaagc ttttcggata caaaaaagtg attgccagtt attgctgaga tcacttgatg
     3241 atttacctat ctacttaggc gaaagcaaat caaaattcgg aatgaacaca ttccatattt
     3301 ttttactaga aaacgaaccc agatgggaaa aaagccagtc ttcgcctggt ggatacctga
     3361 caactaagat gagtacatta gtcactggtt ttttcctcat tcttttagct ggaacaatct
     3421 attatgggag atacactcag ccaaaaatgt cttctcatat aaatgctgct tctcacatga
     3481 caatgaactc aactaacgat acgcatatac aaaagacaaa tgaagggttg ttagctgatc
     3541 ctaagcaaaa ggtaaatttg acaacacaaa agataaatga tctcaaactt gatacagaga
     3601 cgcactctct ctatatgaca acagatagtg gtgctaattg ggtatttgtg ccgattgatt
     3661 cgcaatggtt gcgttttggc gaatacaccc tgacaacggg gctgattcct gaaggttatt
     3721 ggatggacaa cacatatgat atttccgccg atttctcatg gtttatctat tcttctgggg
     3781 atgatgtacg tctgctgact tcaagagata atggtaaaac atggcaaacg tctattatca
     3841 cccaatcagt tcagccgata cgttatcgaa aagcggtatt ttatggcaac gatggtctat
     3901 ttatctgtac atcagccagt gggatgtctg cagaaacttt gtacatttac actactatgg
     3961 atggcggaat cacttggata aaaagtggaa gtacaatgat caatcagcca gtacaaaatg
     4021 tcagcttcgt tacaacttct ttaggttttg tttcgacacg tgaaagtttg ttttatacaa
     4081 acaacggagg acgctcattc aaagaatcag ttctgtcgat tccggaaaac tatcagacag
     4141 gggggataga tctttttcaa tcaccaaatg aagttactca aataagcgcg aatacgttag
     4201 agacaaaatt ttatttgttg aaaaaaggac caatcgatgc agggaaaatg tttgcctgcc
     4261 tcttccgttc aactgataac ggggaaacat ggcaattcgt tcagcaacta tcgcaagtag
     4321 aacaaggaga ttaaagaaga gagattgtga gcctgacgtt cagcaccgag tcacaagaaa
     4381 caaattcaaa aaacagcttc tcatatttgt tgaatagtgt aactcgtgat tcggagttga
     4441 caagagcaac aagaagcgat tttgaagtat agcttctcgt attttacaag atcgtgtctt
     4501 attgccgata ggttgtctct tgaacaccgt ttattcgtaa aaagggattg tgacaagtct
     4561 tttgtcacta cttaccgaac gaaactgaag gagctatcca aaaagtatca ttagtattat
     4621 tctttgtgtt tctagtggca ccactagtac atttaaaaaa gctatcgatc atggactaat
     4681 catacatgat cgatagcttt ttagataatg tttgttacgc tatctgaaat tgactattat
     4741 aaagactggc ataaagcgtt ggtgattgga gtagttcaga atgtgttcct ttttcgacga
     4801 tatcgccatt cttcattaca aggatcatgt ctgcattttc aatcgttgac aaacggtgtg
     4861 caatcacaaa acttgtccta tcttttgtca cagcttccat tgcttgctga atcaatgctt
     4921 ctgttcgtgt atctacgcta gaagttgctt catctaaaat aacgacagga ggattcgcta
     4981 gaatgactcg agcaattgtc aataattgtt gctgtccttg agataacgtg ccgttttcgc
     5041 tagaaatgat cgtatcatat ccgtctggca gtgttcgaat aaagtgatca cattgtgcga
     5101 tttcagccgc ttcgataatt tgttctcttg tggcatccat tttcccgtag gcgatattat
     5161 cagctaccgt tccttcaaac agccaggtat tttgtaaaac catcccaaaa gaacgacgta
     5221 gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa ttcgatcttt cctttattca
     5281 gttcataaaa acgcatcagc agattgacta aagtcgtttt tcctgcacca gttggaccaa
     5341 cgatcgctac cgtttgtttt ggctttactg aaaaatcgac ttgattcatc aatattttat
     5401 ctgatgtgta accaaactgt acatttttga aagctatttt tcctttcggt tgttctaaaa
     5461 gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc taatatctca aatatcctat
     5521 cgattgctgc cattgcggat tggatcgagt tgatcacata agaagcggta gaaattggtt
     5581 cagaaatctg attgatgtac tgaagataag cttgtaagaa gccgatcgtc acaccacctg
     5641 atagaacaag catagcaccg aataccgcac ttatgatgaa ggcgagttga ttaatgaaac
     5701 gaatggccgg atagatagca cagttcataa attgagcacg tttaaaagcg ttgtattgct
     5761 ttttatttac ttcgtgtata gcagcttctg catgagcttg ctgattgaat gttttgacca
     5821 ctagattacc agctaagatt tcttccattc gattatttaa aacacccagt tcatactgat
     5881 tctgttcaga aatctgtttg tttttgttcg caatcttagt tgtaacaatt gaactgccaa
     5941 cgatcagcaa taagacgagc aaagtaagct tgctttcgat ataaaacagc atcagaccag
     6001 cagcagctat tgttacgaca gaagtaaaaa attgattgat acctgtcaat aacacttgag
     6061 aaagttgatt gagaccggta gttgtccgac taatgatgtc acctacttga tgattgtcaa
     6121 aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc ttttcgcacc ctcaatgtga
     6181 tcttttcgct taacgctgcc attgtccgtt cctgaataaa tgaagtgatg ctgcttaaga
     6241 ctgaacagcc gatcagcaat aagacaggga acaacaatgc ttgtttgata tcactgaaag
     6301 aaagctgtcc aatacctgtt tttcttattt ctgataagag atcatcgatc ccgattccca
     6361 tgatcaaggg catggctacg actaataggt tcccgaataa actgcataga aacaatccgt
     6421 aaaaatattt atgttcaggt ttgatcattt tccaaaaacg tatcaaagca cgcgtgttca
     6481 acgttttttt cttttccatc gtatcccttc tttctatatt ccttgtgatt ttgcaaactc
     6541 ctgataagtt tggcaattag acagtagttc ttcatgagta ccttgtccaa cgatttttcc
     6601 ttcatctaaa acaatgatgt tatctgcttg ttggattgta ctgaggcgtt gagcaacaat
     6661 cacgagagtt ttctccgaca tgtgtttatg aagggctgct cttaatgctg catctgtttt
     6721 atagtctaaa gcggagaaac tatcatcgaa tacatagatc tctgctggtt tgatcaacgc
     6781 tcgagcaata catatccgct gtttctggcc ccctgaataa ttagaaccgc cttgagcaac
     6841 aaagctgtcc aatccgtcag gaagggtgtc cactagttct tctaattgag cgatcttgat
     6901 cgctttagac aactcttctg ttgaggcata ttcatttccc atctgcaagt tgcttaaaat
     6961 tgtcccgctg aataagaaag ccttctgagg gacataactg atgtgggagc ggatctgttc
     7021 ttgagtcatt tgagtaagag gaataccacc aagggttact tttcctatcg tagggtcact
     7081 aagtctcaag agaagtttcg ccaaactact ttttccagat ccggtcccgc caacgatcgc
     7141 tgttgttttt cctttaggta ttgtgaaatc aatatcttct agaactggat cagagatttc
     7201 agtatattta aatgtgacat gttcaaaagc agctgcattt gtctgatcaa aggttggttc
     7261 agctactgtt ttgatcgtct cttcctcaat ttcgatctct gcttgcagta tttcttctat
     7321 ccgtcgtaat gaggcaaacg atttagggat aatgaccaat accatagctg cgatcatcaa
     7381 gtaagcaagt gtcagcatag agtattcgac tacagcagtc acgacaccga tttgtaatgc
     7441 tccttctgct acaagatttc cgccaaacca caaaatagaa gcataaacga ttcccatgag
     7501 caaaaaagca attggagtga cccaagcaaa tattcggttc actttgatca tttgattagc
     7561 atagtcagca aagctcttat tcgttcgctt ttcttcatat gattgattat caaaggcacg
     7621 gatcatatta atcccagtaa agaattctct caatgtgatg gtgatccgat ccatttttgg
     7681 ctggatactc aaagataaag gtgcccctcg tttcatcagt cgataaacaa tccatacata
     7741 aaaaacaaca gagatcaatg taacccatgt tagtaataga gaataacgaa acatcatgag
     7801 aatggtgaaa acgcaaataa ccggtgctgg taagatcatt tggcaaaata aaacaatcat
     7861 ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa agagatccag aactaaaacg
     7921 actgatatcc ttcattgaca gctgttggaa tttacggaaa tatttctccc gcgtttggaa
     7981 tccaaaccta gcggaagcca atgctgaaaa gtaactagag gcaactgcag cgattgttcc
     8041 tagaaacgct acaagcagca tttcaactcc aatttttttt ataactgagg cttcgccact
     8101 tgctatacct gtgtcgatca gctttgctac taattttggt acacctaatg taccggcgat
     8161 ttctaaacag atcatcacaa aggtaaggag agcgagtcct ttgtttttct gtaagaatgt
     8221 ttttaagata gacatattat tcctcctttt ttcctgatac ttttcgattc tataccttca
     8281 agtaacttga aggtcaatag aaaaaagaat aaactgaaaa agaggtgaga aaaaatgaaa
     8341 aaaatgtata cgattggcca agtatcggaa ctatttgatt tgccaaaatc aacgatccgc
     8401 tattgggatg aacaaggatt aatccgttca agtagacaag aagagaatga ttatcgattg
     8461 tttgatatcg atgatatttt tatgctttat gatatcgttt tttatagaaa attagatata
     8521 ccgatcaaac agatgaagaa tttatatgga aaaacattga cggagttata tgagacactt
     8581 gatgaaacag aaagaagaat ccataaagaa ctggtggtga tgaaacaaaa acaaaaagag
     8641 ataagggagc aaaaaaaaca gttgaaattg atgatcgata caaacgagga agaatttcct
     8701 gtagaagaga tcccttttga ctgtatgatc tcaacggagt ttgaagatat tgtggaaatc
     8761 aaaaaatttc tacctaacta ttcaagcttt gggatgatga gtatgccagc ttccagttca
     8821 cagacgatgt atggattttt tatcgaccct agtgaagtac atttatttac tcaagacgtg
     8881 atttgggaga aaaaagatac ggctgtttat cgtcgattct tattgaaatc cgagatgaac
     8941 catgcggaac gtaataatat tttagaaatc agagaaagaa tgtatgaaaa aggatggaaa
     9001 acaggagaag tgatcggcca gtatctattg acgaatacgg atgaaaacaa tatcagaact
     9061 gaatattatc atgcatggat agaaatgaaa aaataggaaa tatgtgttta agataactcg
     9121 aaaatgatgg ttgttttagt tataaagaaa aattttctgg ttcgattacg ggtttaatgt
     9181 gggggattat tagtcgtgga tagatagaat tttagttttt tagaggaaat atgtctttaa
     9241 aaatggaatg gttgcaattc ttagcgatat atacgatgta attgtcctct taaaaaagta
     9301 tggaaaaata attgtattgg gtgttatgta taattcatgg tgaactgaga ctaatgagaa
     9361 gaaaaattca tattgttatg agtgagattg tatcagatga gaatgataaa ttaaataggt
     9421 gcataaaagc attacactta aaatacattg ttgcttacat tattacaata tgttaataag
     9481 gcaaaaataa atagatggat tgaataaaca tattttttaa tcaaatagtc cctactgtga
     9541 ctttcctcag ctatcggtga tactaccgat agcatttttt tgcttataaa ataattataa
     9601 gtatttagag atttttcttc taacattctt caaacaagaa aaatcacaaa ggttattgtt
     9661 atttttatat attcttttta agaatgattg tttttttata gaaattttaa aaaataaaac
     9721 acgttattga tggatagtta gtttgttatt tggtaatatt atagaagttg actggtagtg
     9781 atttattttt cgtcgtttta taaaaatgtt attttttgga aaaattcaca aatatgtaac
     9841 gaatgttttt ctggaatttg ctgtgttaaa aagcagtatg actaatataa atactattgt
     9901 catttatgca attttttatg gatagtaatt ttagagggga atcaatgaag gaagaataga
     9961 agggaaaaat agttgcaaat acgccagtta gcaaaaatag caatacatct tcaagcttta
    10021 aaattcgaga gaatcatctt ggtagtggaa tcaactacaa caaaaggatt aaaccaatta
    10081 atagatagaa ttaccaatgg aaaaatagaa aaggtagtta ttctatacaa aagttgttta
    10141 attcgcttcg gttatgaact tagtgaaacc ctttgcaata tatggtacag aatatagaaa
    10201 ttattgacat tacagaaaat gatgaagatt taatacagat tgtcactatt tgtagatacc
    10261 ttttacaagg aaaccgagcc aataaagcta agaagatgat taaggagttg atggaagacg
    10321 atacaagtta agaaagtgaa actacgccca aataaagagc aaaaagagta attggacgtg
    10381 gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt attagtaatg acggtttgag
    10441 aacatccatg acgagaatga aggaacatcc taaatgcgtt tagttgaacc aagtgccaaa
    10501 taatatggcc aaataagcga tcagttatcg atgaatccca agtattctaa actatatatt
    10561 cattttgatg ctaagcattg atatattttt ttgggattga tgaaggttaa ccaatacagg
    10621 gactcactga tgaaattcta gagtctgacc ttggtatcaa cgatttagtg attgtttcta
    10681 atggaaaagt ataaaagtat ataagtacat taacaaaata actattaggg ttacgctcca
    10741 gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata attatggtta agccacacgg
    10801 acaactaaaa gtcatacaaa gctatcgaat caatcaacag aagacaatcg gcaatcatta
    10861 gctttgttta cagcttacat tgcaacgctt gagttgaacc gaaaatacga gggaggtaac
    10921 gcagccattt tgagttgggg aatcttttta gaaaattatc gggtaccttg ttgtttagtc
    10981 aaaattataa gggggagtaa tagtgaggaa caaatttcat aaactgttaa gtatgtttct
    11041 agttatgctt attgtgatca atcaatttgg gaacataacg gttctagctg agtctttagc
    11101 agaacagcca gagcgttcgc tgacgatcga agttgatcaa gaggaggctg ctttgggtga
    11161 gcagttccag ttgcgagtca gaggaacacc agacctgcta aatgcattgg tcattgataa
    11221 gagtgaggat ttgattgaag gggaagtgaa gcaactcaat gattacgaac gtctgattac
    11281 ttattcttcc cagcattcag gtaaatacga attgaccgca agaagcggtg atattcaatc
    11341 aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc acaagtgagt cttttaatgt
    11401 accagaagaa agcacaagtg aatcttttaa tataccagaa gaaagcttaa gtgaatcttt
    11461 taatgaagcc agatcatctg agccatctat aacgcaggaa tctcagattg aaagacaaga
    11521 ggagctcttg aattatgaca tcggcatctc tgctgagcgc cccactttgc cgagcaggca
    11581 tacacaaacg ttaaagggat acattcaagg aggcgctggc gatattcttg aagaatatgg
    11641 ggagatggtc gtacgagttc ctagagcatc gattggctta attgagttca ttagagtgga
    11701 ggtcgctgat gattcacctt taaagccact ggcattttac caagaaggca cagacgttgt
    11761 ttttccattt acctttgatt atgaaggatt aaatgaagcc gctcagtcgt atacattcag
    11821 tatcacgttt gaagttgctt tctttcaatc aggcggtact gagccaaata atgactttca
    11881 agtgagtctt acttatgctg agcgagcagc aaacacggaa ttttcaatta cgcctattcc
    11941 ttcatcaaga cccatttttt cgaaattata ctatggctct agagaaatcg acagccaagg
    12001 aaatcttggg aatctgctga ttgacgggca agacgtgacc aacagtaata aatttatttt
    12061 gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg gttaccgatg tactaccgca
    12121 aggaacaact ttggcaccga atccgactac atggagttcc actggaaata gagaacctat
    12181 ttcaggtggg gttcggatcg cagaagtaac gaatccaggt gaagcaaacg ttagaactca
    12241 ttatgtttcc gaggaatttg tggacaaagt atcttttaat aaggaaacga atacacttgt
    12301 tgtggacttt ggtaatctag cagagggtca atcgtatttt attgaatatg ctcttgaagt
    12361 gaccgatgtc gacttgttca atgaaggaag acaagaggat gggttttctc ggaatcgagc
    12421 gagtatgacc tacaataatc aggtaatgag ccgagactac cgcttgagaa tccctgaaag
    12481 taatcttccg gcaatctctt taagaaaaag tgtggacaaa aatgttatca atctcaatga
    12541 aaatgaacta acgtatactc tgcatgtctc gatgttttct ggaacaagaa atacgcttgt
    12601 tattgaagat gttttggaag aaggcatgga atttgtgtca tttctatcag atattccctc
    12661 tttcattgat gccgaagtca ataatggaat agttcgtttc agtgcgaata atacgattgg
    12721 tggtccgaca agttttaccc tctcttttaa ggtcaatgta gaggcgtatg cggtcggcga
    12781 tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat cgattgaata cgagtactgt
    12841 aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa actgatgaga atggccaaag
    12901 gctggcagga gcaaaatttg aaattttatc aagcgattca caagtgatac aagaaggtat
    12961 aacggcagaa aacggagaat ttttaagtga accgcttcct gtaggtagct atcaagtccg
    13021 agaaacagaa gcaccggaag ggtatctttt agatgcgacg gtacatgatg tttttataaa
    13081 tgaatactcc atagaagaag tgacgttaac cattgaaaac atccttgata cgggtggtgt
    13141 tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt caaggagctg tttttgagtt
    13201 acagaaccga gaaggagaaa cgctacaaac cggt
//
LOCUS       DAYYAJ010000083        13114 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000083 DAYYAJ010000000
VERSION     DAYYAJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.083"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..145
                     /locus_tag="ACHATZ_001967"
     CDS             <1..145
                     /locus_tag="ACHATZ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8404487.1"
                     /translation="LVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRKR
                     VSR"
     gene            complement(207..2018)
                     /locus_tag="ACHATZ_001968"
     CDS             complement(207..2018)
                     /locus_tag="ACHATZ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8404488.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2015..2380)
                     /locus_tag="ACHATZ_001969"
     CDS             complement(2015..2380)
                     /locus_tag="ACHATZ_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8404489.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2543..2938)
                     /locus_tag="ACHATZ_001970"
     CDS             complement(2543..2938)
                     /locus_tag="ACHATZ_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8404490.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2956..3918)
                     /locus_tag="ACHATZ_001971"
     CDS             complement(2956..3918)
                     /locus_tag="ACHATZ_001971"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8404491.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3918..4130)
                     /locus_tag="ACHATZ_001972"
     CDS             complement(3918..4130)
                     /locus_tag="ACHATZ_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8404492.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4151..4849)
                     /locus_tag="ACHATZ_001973"
     CDS             complement(4151..4849)
                     /locus_tag="ACHATZ_001973"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8404493.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4869..5411)
                     /locus_tag="ACHATZ_001974"
     CDS             complement(4869..5411)
                     /locus_tag="ACHATZ_001974"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8404494.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5543..6547)
                     /locus_tag="ACHATZ_001975"
     CDS             complement(5543..6547)
                     /locus_tag="ACHATZ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8404495.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6544..7533)
                     /locus_tag="ACHATZ_001976"
     CDS             complement(6544..7533)
                     /locus_tag="ACHATZ_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8404496.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7530..8336)
                     /locus_tag="ACHATZ_001977"
     CDS             complement(7530..8336)
                     /locus_tag="ACHATZ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404497.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8502..9458
                     /locus_tag="ACHATZ_001978"
     CDS             8502..9458
                     /locus_tag="ACHATZ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8404498.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9535..10053)
                     /locus_tag="ACHATZ_001979"
     CDS             complement(9535..10053)
                     /locus_tag="ACHATZ_001979"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8404499.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10141..10290)
                     /gene="rpmG"
                     /locus_tag="ACHATZ_001980"
     CDS             complement(10141..10290)
                     /gene="rpmG"
                     /locus_tag="ACHATZ_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8404500.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10518..10964
                     /locus_tag="ACHATZ_001981"
     CDS             10518..10964
                     /locus_tag="ACHATZ_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8404501.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11159..13054)
                     /gene="asnB"
                     /locus_tag="ACHATZ_001982"
     CDS             complement(11159..13054)
                     /gene="asnB"
                     /locus_tag="ACHATZ_001982"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8404502.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4072 a         2796 c         2249 g         3997 t
ORIGIN      
        1 attagtagga agtctggcaa cagccgctgc cgttgctggt ttggtcgctt ctgtgaaaaa
       61 aacagtgatc gatccaattg acgaaaaaga agcaatgatc gaagaaaacc gtaaaaaagc
      121 aatgcgcaaa cgcgtttctc gttaataatt atttaaaaac acgcatgaag acttgtgaaa
      181 gaacagataa gtcatcatgc gtatttttat ttgtcagtca atataagttg tgctatttta
      241 tctgcctgtc tcagattcca tggatctgta taaggtctag ctactgctgc agttagccaa
      301 tccacacctt caacaggtat tccatagcaa taaaagtcag actggatttc ccctgaagca
      361 tcaatcacgc gattcgtacc tcgctcaaca tctatagctt ctgaatgata acttcctgta
      421 ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct tcaaattttt tatgacagga
      481 ttacttgaac gagaaaagct tgtttgtggc aatcgagctt caatcaaata gcgactgctg
      541 atttctttat cttgtagaga aataacatag cggttgtgct tttgctgaat atcaaatggc
      601 ggttcgaaca aatgaaaaat acccgcatct atcagtgctg ctaattcttt cgtccgaatg
      661 actggcggac cgatcgttaa aaagctattt agatgagtaa accatcccca tagtatttct
      721 ttgtattcct ttgcagaaaa aacgtgccat tcaatagcct gccggattgg atcacgccag
      781 tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc aattgccttt ttccgcttct
      841 tttatctgcc cttcaataaa tgctcggcta gcttctgcaa aggactcatc ctgataggta
      901 agaggaattt ctaaatacga ccaatcccat ctataaggga ttagtgctgg atgctttccg
      961 agacactcct ctgaagagag ggagagaaaa tcatgctcaa aatttttctt tggaatcggc
     1021 aaatgatatt cttcgatgat tttcttgtaa taaaacaact caacgtcttt tttcagatag
     1081 tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc attgctccag gttttctttt
     1141 gttagtaacc gtggccatgc cattgatccg ccttgctttt ggtttcttcc tcgagggtaa
     1201 taagggagcc cccttcttga tccactgtat acaatcggtt ccttgcctga tggatgatag
     1261 acaagttttc ctttttcgtt ctcgaagcgc ccttctcttg atactgtaaa caatccgaca
     1321 taatcaaaaa aacttaagcc tagacctctt aaaaagacat tttctttagg cggtatcttc
     1381 gaaagatcga catctgcagg attagaagga agctgataca gtaaatcatt tttctttgcg
     1441 tagtctaccc acactctttc ttcttctgtc tcatcatttt cccagtgacc gcttgctaaa
     1501 acaagctgat cgatagaata gctttgctga tctgtctgta aaagataacc atcttttctt
     1561 ttgctgactt tttttactaa agaataaaca aactgaatag aagctggaaa ttcttcttgc
     1621 aactttgaga aaaaccagcg ttgatagagt ccgtataaac atcgagtaga ttgctgatct
     1681 ttttccagac gattgatttc ttctataatt agttgcttat ttttggttac cgtctgttcg
     1741 atataatctg tagcaggtcc tttactccat tgaaacaagt tgggtccctg tgttaccaat
     1801 cctccactgg acaatgtttc atctgtaaaa agcgtcactt gttgagaaac agaattcatt
     1861 aataactccg gtggctgatt caaccgccaa actcgacctc caggaccatt gggatcgaaa
     1921 agaaaaagtt caagagaatg tctgtcctga ttattatgga gaatcctttc tgctatactt
     1981 aatcctctag gtccggcacc aatgatccct attttcattt gcatgcctcc atttcgataa
     2041 attttttcaa taaaacagca tggttctgat gttcattttt tgcaccataa acaaaagtaa
     2101 tgactggatg ttctttcata agttgactga gtttaaaaat agcttgcttc ttttcttctt
     2161 cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc aaatcgttct ggtacatggt
     2221 caaaagtttt gcgtgcttca ttactaggtg caatttcttt caaccacaga tctaactgcg
     2281 ctttttcttt tgaaattcct cgtggccaga acctatccac gagtattcta tagccatcct
     2341 ctttttcagc atcaaggtaa acacgtttta atttgatcat agttgtttcc ttctttctgc
     2401 cttttcgctt agcttatata tgtagataat agcacaaaat aaaaaaaatg aacggcacaa
     2461 ctatccattg cgccgttcat gtgatcttgg tcaaaatcta atgattgatt cggtttttaa
     2521 agctgtcata caatcatctg ttttatactt ttttactttt gatttttcca gaccatccgt
     2581 catatccacc cttaagtaaa taaatctctt gataacctgc tttacgtaat ttatttgctg
     2641 tacggatgct caagcttttc ttttgatcgt aaatatagac aggctggtct tttcgaatag
     2701 atcctaatga atctttcaaa acagtgtaag ggatgtttct tgcacctaaa atatgcccag
     2761 catcaaacac atctttttcg cgaacatcga ttacttgtgc ttttctcatt ccttctcgaa
     2821 attcttcttc tgtcaaagtc gttgctgaac gcttggccat tactcgtaga tagacttccc
     2881 aaaagatgat tgcaagcaca atcaatagca aaatgatatt aattacccat aaaaccatgt
     2941 aatgcctccg tttttttaag caaatttcaa ggctaatgag gcagcaccaa tgacaccagc
     3001 ttcattacca agctctgcta atttgatttt tgttgattct cttacttgcg ggaaagtaaa
     3061 ttctttaaaa tatttctcta cacggctacg taaaaattct cctgctgccg atactcctcc
     3121 acctaacaca atacttgacg gattcaatgt attccccaag ttggcgcaag ctaaacctaa
     3181 gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga tcgtcttctt gagccatttc
     3241 aaagatatct ttacttgtta cttcttcacc gttgtctaac attgctttca gttttgaatc
     3301 tcctgcgtat tcttctgcca agtaacgacc tagacgtacc acaccagtag cgctagacac
     3361 agtttctaaa catccgcgtt taccgcaagt acattcgaac ccttcaggat ccacagtgat
     3421 atggccgatc tctccgcctg ctccgctcac gccgtgaatc aaatttccgc cagcaacgat
     3481 accaccgcca acacctgttc ctagagtaac gaatacaacg tctggatcgt tttctccagc
     3541 acctttccat cgttcaccta aagcagcaac attcgcatca ttatcgatag cgaatttgat
     3601 tcctgttcct gcctcgatca gttttttcac aaattgaact tctgtccagt ttaagttata
     3661 cgcaccaata accgttccag cttcgctgtc cacgcttcct ggggtaccca tgccgattcc
     3721 gataaagtct tcggctttca tattatataa attcaaacga tggttgattg attcaatgat
     3781 ttcaggaacg atatgcttac catcatccaa aatattggta tcgatgctcc atttttgttg
     3841 aatctctcct tctggtgtta aaattgcaaa ttttgctgtt gttccaccta aatcaatccc
     3901 aataatcttc ttgtccatta caaatttcct ttctttcggc tttcttcaat tcggtgctct
     3961 ctttttaata tgcgccacgc tgtcatgtat gtatcatgat caatcaaacg gctatcatac
     4021 agattacgca attcgatagt cattaattca atgtcataga tgcgtgcacc cacatagata
     4081 aagatgccaa attttttgaa cagctgctgt acatcatata agctctccat ctttaagcac
     4141 ttccttcata ttatacaagt aaaccatatt tttttaaacc cagtaaaata catattacaa
     4201 aaagaaatac aaaaactagt gtagaaagaa tcctttggtg tatcgaatag cttgtctgtt
     4261 gtttcggtag acccaaaaca ttccctaaca ctaatccgcc aattagccca ccgacatgtc
     4321 cccaaacatc tacagaggta tccagaagac caaaaacaaa tgtcaacaca ataaataaaa
     4381 gaaaatgtct gactagctgc tggatcgctg ggttatgttt aaaatgaaac cctaagataa
     4441 acaacgcacc gaacaaaccg aatatcgaag tactcgcacc accagacaga acattcgctt
     4501 cgttaaaagc aaagcttgct gtatttccca ttatcccgct gaacatataa atcaagaaaa
     4561 aacgccaatg gccatagatt gcctcgattt gctgccccat aaaatacagt atcagtgagt
     4621 tcattacgaa atgcatcagt ccaaaatgga tgaagattgg tgtcactaaa cgccaccatt
     4681 cattgaagtg aacaagataa ggaccataca ttccggcata ataagggatc tcaagtcgcg
     4741 gaaataattc catcatgagg aagaccgctg tctgtatccc taaaaaagca taggtccata
     4801 acggtcgtct taaccatgca cgcaatttta tttgctgttg ataattcatg aaaacctccc
     4861 atttcatttt agtcaataaa caattgttgg atcttctgat caaagctctc cggttgccat
     4921 tgttcattca attgttcacg aaaaacaagg ctacatgttt ttttatgaaa attatttaaa
     4981 taccggtcgt aataaccacc gccaaaaccg attcgataac cggctctatt gtagataata
     5041 cctggtacaa tgaggagatc gatcatctcc ggcaaataga gcttctctga ttgtggctca
     5101 agaacaccaa aactcgaacg tacaagaacg gactgttcat ccatttcata aaacgccatc
     5161 tgacgtttcg gcaaagtttt aggtacaact attttctttt gttctttttc tgctcgttgg
     5221 ataaccggct gtgtatccag ttcaaattca ttcgataaag tcgtccctat cacttttgct
     5281 gacttccagt actcgctggc gaatagctgt ttatagattc gctgttcttt ttcctttttc
     5341 ttgccacttt tggccagatt tttcagcttt tcgattcctt tccggcgtaa tcttactttc
     5401 aattggtcca aaaactcacc cttccttctc tcataaaatg gtataggaaa aaaagaaaaa
     5461 tagcaatcga actttcaaat aaatgcataa atagaataaa aagaagacct agaaatgatt
     5521 ggccttcctt ttttaataaa tttcaactgt tcagcgcgtg tttttgtctg ataaaaagtg
     5581 catacagaaa atatggcgca ccaaaaaggg caatcaaaat tcctgtaggt acttccccac
     5641 ttggtagaat cactctagca accatatctg cactaagcag aaaaatggaa ccgaacaaac
     5701 cgctcagaac aaaggtccag ttattttctt tacgtacgac tagcttcgca gcatgaggag
     5761 ctagtagtcc tacaaaagat aaactacctg tcaccgctac actaccgctt gccaaagcaa
     5821 cagctaacaa caaaaggatt ttttgacttt tagccacgtt aaggcctaat ccttgcgcgc
     5881 gttcttgacc aaatgatagc aatccaatcc tctgtgcata aagtcctcct agcggaatta
     5941 agataaccgc ccatggaagc aatgcccaaa cataaggcca gctcgcaccc cagattgatc
     6001 ccgctaacca tgcattcaca aatccataat tttccttaga taattggatc gtgcatagca
     6061 atgttaatgc agaaaggccg gcattaacgg caataccgga caataagatt cgattcatac
     6121 ccaaaaagcc atttcgatca taggccgcaa gatagacaca gcaagcagct aaaacaccac
     6181 ctagaaaggc tacaactagc aatagaaacg gcagcgaata gtgcgaaaag aaacctaaat
     6241 aaagcatcac taaaaatcct gctccggcat taatgcccaa tatactagaa tctgccaatt
     6301 catttcttgt tactccttgc aagatataac cagaaacgcc aagacaagct cctgctaata
     6361 aactgatcag taatcgagga actcgaaatt ctctaacaat cagcgccaaa gaagatgatt
     6421 ctttccctat gaacgtggca aaaagctgat ccaggtaaat aaaaggcgta ccaatcatta
     6481 aactacctat agacaggatg acaagtacaa aaagcaagaa atagaacgtc caatatattt
     6541 ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac ggaacgccaa tcaatgcaat
     6601 gactaatcca aaaggtgttt caaaaggggg attgatcatc ctcgctgtaa aatccgccaa
     6661 cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga acataacgat agtccgttcc
     6721 aaccattccg cgaacaactg gcggaaccaa cagcccaata aatgaaacag gtccgactaa
     6781 cgaaacggca atggctgcta acactgcaac tagacagaaa acaatcaacc gcgttcgttt
     6841 aggatgactt ccaagagcta atgcatgtgt ttcactcaaa tgaatcaaag atattgcttt
     6901 tcctaatgaa aaagaaacca aaacagcaag tatatataaa ggaaacaata ctgctaattg
     6961 tttccatgtg atattggcag ctccgcctac aaaccaaaaa gcaagatcct gctgcagatt
     7021 aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa gaactaatag ctacgccagc
     7081 taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac tgcaaaaaac caaatacgag
     7141 caaagttaca gcaaaagaac ctgccaatga cattagaaac acagaaaaat cagaagcttt
     7201 aggccaaaaa gcaaaactaa gggccattgc caacgaagca cccgcgttga ttcctagtaa
     7261 gcctgaatct gctaaaggat ttcttgtcac tccttgcatc aatgcaccgc tcaatgcaaa
     7321 acaactccct acaagaaatg ctcccgccac acgaggcaaa cgaatatttc gtatgatttg
     7381 gtgactttgt tttgatctat caaaatcaaa caacgcctgc caaaaatctg cttttcccac
     7441 agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc aaaagaacaa aacagaccaa
     7501 aagcaggagt ctatttttct tcatagtcct cataattgat cctcctgctc ttttggcaac
     7561 aaatcatagg ttagaaaaac tggtttattt gttcttggaa gcgtgacaat cgttgcatca
     7621 atctcaaata tttttttcat acactctttt gtgatcactt cttcaggtgt accttctgtg
     7681 atcaacttac cgtttttcat tgctaataaa taatctgaaa aacgtgaagc atgattaatg
     7741 tcatgaatcg acatcaagat cgttgtttgt ttcatttgat tgatttcagt caataactgt
     7801 aggatctcta attggtgtgc cggatctaaa aatgttgtcg gttcatccaa gatcaagata
     7861 tctgtttcct gagcaagcgc catagcgatc cagacacgct gggtttgtcc accagacagc
     7921 gttgcgatat cccgcatacg aaatccagct agacctgtag catccaacgc ccactcgatt
     7981 gcttcatgat cttgcgtgtc catgccactg aacagttttt gataagggta tcgtccgtaa
     8041 gatactactt cttctactgt catacctgta gggtgttcac tagtttgtgc taataatgta
     8101 attttttttg caagttcttt tgaactgtat gtagagatat cagagttatt gacaatgatc
     8161 tttcctgatt tcacaggtag tatacgggta aaacttttca ataaagtaga ttttccactt
     8221 ccatttggcc caatcaaact agtgattttc ccttcaggaa tcgtaacaga catgttttca
     8281 atgatttttt tgttttcgta gccggctgca atgtcagctg ctcttaatct cgtcatttca
     8341 cccatccttt cgctgaaaat cattctcaat tatgacgctg tttcaacatt tcgtcaagcg
     8401 ctactttcat atctatagcc aaatgaagcg gttgacagcg ttcgaaatat caggaataat
     8461 aaccaatgag aaagattctc aattatgtta ggaggaaaga aatgaaatta aaaacaaaag
     8521 tattcacatt atctagcata ttgctgctgg ctggagtact gagcgcttgc tccccctctt
     8581 cagataaaga aacagctaca actgccaatt cgacgacttc atccaatagt actcatacag
     8641 tcacagatac acttggacat aaagtagaga ttccaaacgc gcccaaacgg atcatcggca
     8701 gttacttaga agactatctg attgcactag gggaaaaacc tatcgcccag tggactgttg
     8761 gcagtggttc cattcaagat tatctgcaag atgatttaaa ggatgtccca acgatttctt
     8821 atgaccttcc ttatgagaaa gtgttaagct ttgagcctga tttgctgttg atttcttctt
     8881 ccgctactgt cgaaggcggg aaatacgaac aatatagcaa aatcgcgcca acttatgttg
     8941 taaaaaacgg aacagatgtc acatgggaag aacaactaaa agatgtcggc aaagctttgg
     9001 caaaagaaaa agaggcagaa aaaatcattt ccgattatca aaaatccatc aagaaaactc
     9061 gagaagaact agcagataaa atcgagaaca agacagctgc agtgttgtgg gtgaccaaca
     9121 actcagcctt tatggtcagc aaaaatcggt caagtggaag aatcgtatac gacgatctgg
     9181 aatttggtgt tcctgatcta gtggaagagg tctccaaaaa agccacttct gactggtctg
     9241 ctgtttcctc tgaaaagcta gctgaattag atgctgacta catcatcctt gtgaacagtg
     9301 acaaagatgc tgccatgttc aatgaaccaa gctggaagaa tcttaaagca gtgacagaca
     9361 atcatgtact gcaattcgga cctgaatcta gttggctata taacggacct attgccagtc
     9421 aggaaatggt caatgatatc aaagaaaaac taaattgatt ttaactaaaa aaagaatctt
     9481 cacgattagt cgtgagatgc tttttttctt attaacaaac tagacgcata gattttattc
     9541 ttcttttgaa gcaaccctat tttttactct ttcccatttt tgaaaacgat gggcatatag
     9601 attttcttga tccgttatcc cctcatactc ctctataagg gcgaagtctg aaaaatcaat
     9661 attcccaata tacgtatcgc cttcaaacgt gctatcgatc aatgttcgcc agatccgacc
     9721 agtgtaagga agaagttctt gaaaaatccg actaccaccc gaaacataga tatcttctga
     9781 gatcgtttga ctatacgaca aaagttcatc gatcgaatgc attacttccg cattttcttt
     9841 cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg cgataaggaa gtgaaagatc
     9901 ccccattcct tcataggtct ttcttcccat cactaaaatt cgatccattg tatgttcgcg
     9961 aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa ccatttttgc cgatcagacc
    10021 atttttatct tgtgcccaca tggaaataaa catattatcc gctccttatg attttagatg
    10081 ttttgagata aaaaaagtct caattctttg accagcaagg aattgagaac tttaaaaaca
    10141 ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt gggcaatatt tttgtttttc
    10201 caaacgatca ggattgttac gtttgttttt gcttgttaga tagttacgtt ctttacaaga
    10261 agtacactct aaagtgatgt ttacgcgcat gtttttccct cccattttta caagaatcct
    10321 ctaagatgac tctcagactt gattatctta ccatgatttt ttctagattg ctagtgtttt
    10381 tcacgatttt gttaagtaaa actacttgac gataaataca acggttttac ttctcaaaca
    10441 aaatcttttt ctttttgtat aaataccctt acacttgttt atatagatta tgttttttaa
    10501 taaaggggga tactaatatg ccatggaaca tgaaggactt tcctgcatca atgaaaaact
    10561 tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa tgcattatta gatgaagggt
    10621 accctgataa ccgtgcgatc ccaattgcca ttgatcaagc aaaaaaatgg tatgaagatg
    10681 ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa tccaagcaag acggatgaac
    10741 acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga tgtgatcgta gaatatgagg
    10801 aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc aagcaaccat tttgatacaa
    10861 aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa caaagaatca actttgttca
    10921 tttataaaaa agatgggaca aaagaaaaag aaatcgatta ttgaacatat gtaaaggggt
    10981 tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac aatggtgttc ctctgccaac
    11041 tccgaaccat aagtcataat atctataaaa cgggaagagc tgttttttga aattgtttct
    11101 tatcactcag tgttaaacgg taggagaaaa cctctttttt attgatattt gattttcatc
    11161 aaacagtcac atagtcgatt gcttctgctt gcggaatcgt ttcatctaca aagtagactt
    11221 tataccatgt caagaaactg aagattgtcc agatttttct ttgctcttct ttacgtcctt
    11281 cgtgatggtc atctagcaac ttcatgattt tttcttgatc aaaaaattct tttgcaaaat
    11341 ctgcttcgaa taatgctttg acttgttcgt agccgtgttc ttctttcaac caagctttga
    11401 ttggcacagg gaatcctaat ttcacacgat tcgaccattc ttccggaaga tgtttgttcg
    11461 cagcttgtct gaaaatatct tttgtgttgt tttgattcaa taaaaactta cttggtattt
    11521 gttgagccaa tttcatgact tcgatatcca ataaaggtac tcggacttcc aaagaacttg
    11581 ccatagaaag tttatctgcc tttaacaaga tatcttttgg catccattga tgaagatcga
    11641 catattgcat cttattgact tcatctttga ttccttctgt tttctcgtaa tgaacagtca
    11701 tgatttcatc cacagatggt gctgtttgat attttggtgt taacacttct gatgcttctg
    11761 attcttcgaa cactcgagca tgaccgataa aatattcttt tgcaggagca agtgattcat
    11821 ataaatggat ccgtccatgg aaatttttca tttttccgat tttacgtcct aaattatagc
    11881 gtgttccttt tggtaatttt ttcaatcctt gagcaatcac acggatgaat tttgaattcg
    11941 tatggaatcc atacgtttgg tatcccgcaa acagttcatc cgccccttct ccagattgga
    12001 ctacccgcac actttgtgaa gcaagatttg ctaaaaagta caaaggtacg caagaaggat
    12061 ttgaatcagg ttcatctaag tgatactgga tcaaagggaa tgctttgaac gacatgtctc
    12121 cgtcgatcac tgctgctgta ttatctaagt ccaatagttc ggttagtttt ctggcttcaa
    12181 tcgcttcatt ataggtttta tcatcaaacc cgattgagaa agaatgatct ggtttcaata
    12241 cagaggttac ataactggaa tcaaccccgc tggataagaa agaaccaact tctacatcac
    12301 tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc gattttttcg atccattctt
    12361 gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca atattgctga atgtctagtt
    12421 ttccatcttt atacgtataa taatggcctt caggcaaacg atagacatct ttgaagaatg
    12481 tttcgccgtt taatggagaa tattggaaag tcatataagg tttcaacgct tctttgttca
    12541 attctttgtt aaaatcagga tgaggcagaa aacttttgat ttctgaacca aacataaatg
    12601 ttccgttcat ttctgcataa taatatggtt tgatcccaaa gtggtctctt gcaccgaata
    12661 actcattctt ttcattatcc caaatagcaa aagcaaacat tccgcggatc ttttgcaata
    12721 attctgttcc ccattcttcg taaccatgca aaaggacttc tgtatctgca tgtgtttgaa
    12781 aaacgtggcc agctgcaatc aattcttctc tcaaaggctg gtaattatag atttcgccat
    12841 taaaaataat gatctttgtc cgatcttcat tgtaaatagg ctgagtaccg ccctctaagt
    12901 caataatact taatcttctg aatcctaaag caacatgttt atcgatatat tctcctgaac
    12961 tattaggtcc acggtgaacg attcgatcca tcattgtatt aataattgtt tttttgttgt
    13021 ccttgtcgtt aacaaaacct acaataccgc acatttaact ttcacatcct aatataaaat
    13081 tactgtttat ttcattcata tgcatcttat cata
//
LOCUS       DAYYAJ010000084        13084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000084 DAYYAJ010000000
VERSION     DAYYAJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.084"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHATZ_001983"
     CDS             complement(79..2199)
                     /locus_tag="ACHATZ_001983"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8404503.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHATZ_001984"
     CDS             2429..3607
                     /locus_tag="ACHATZ_001984"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8404504.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHATZ_001985"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHATZ_001985"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8404505.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHATZ_001986"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHATZ_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8404506.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHATZ_001987"
     CDS             complement(4960..6213)
                     /locus_tag="ACHATZ_001987"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8404507.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHATZ_001988"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHATZ_001988"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8404508.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHATZ_001989"
     CDS             7790..9136
                     /locus_tag="ACHATZ_001989"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8404509.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHATZ_001990"
     CDS             complement(9444..9830)
                     /locus_tag="ACHATZ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8404510.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHATZ_001991"
     CDS             complement(10098..10247)
                     /locus_tag="ACHATZ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404511.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHATZ_001992"
     CDS             10709..10921
                     /locus_tag="ACHATZ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404512.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHATZ_001993"
     CDS             complement(11199..12998)
                     /locus_tag="ACHATZ_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8404513.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4290 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 ttta
//
LOCUS       DAYYAJ010000085        13056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000085 DAYYAJ010000000
VERSION     DAYYAJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.085"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1080
                     /locus_tag="ACHATZ_001994"
     CDS             157..1080
                     /locus_tag="ACHATZ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8404514.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1086..2339
                     /locus_tag="ACHATZ_001995"
     CDS             1086..2339
                     /locus_tag="ACHATZ_001995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8404515.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2473..3819
                     /gene="gor"
                     /locus_tag="ACHATZ_001996"
     CDS             2473..3819
                     /gene="gor"
                     /locus_tag="ACHATZ_001996"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8404516.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4633..5586)
                     /gene="menA"
                     /locus_tag="ACHATZ_001997"
     CDS             complement(4633..5586)
                     /gene="menA"
                     /locus_tag="ACHATZ_001997"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8404517.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5599..6702)
                     /locus_tag="ACHATZ_001998"
     CDS             complement(5599..6702)
                     /locus_tag="ACHATZ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8404518.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6792..7727)
                     /gene="pplA"
                     /locus_tag="ACHATZ_001999"
     CDS             complement(6792..7727)
                     /gene="pplA"
                     /locus_tag="ACHATZ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8404519.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8029..9963
                     /locus_tag="ACHATZ_002000"
     CDS             8029..9963
                     /locus_tag="ACHATZ_002000"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8404520.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10420..10836
                     /locus_tag="ACHATZ_002001"
     CDS             10420..10836
                     /locus_tag="ACHATZ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8404521.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10850..11416
                     /locus_tag="ACHATZ_002002"
     CDS             10850..11416
                     /locus_tag="ACHATZ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8404522.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11439..12425
                     /locus_tag="ACHATZ_002003"
     CDS             11439..12425
                     /locus_tag="ACHATZ_002003"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8404523.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12580..>13056)
                     /gene="scm"
                     /locus_tag="ACHATZ_002004"
     CDS             complement(12580..>13056)
                     /gene="scm"
                     /locus_tag="ACHATZ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8404524.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4065 a         2369 c         2696 g         3926 t
ORIGIN      
        1 ttttgtcaca atctcttttt atatttggac agaatatatc gaactttatt ttgatttttt
       61 gtgaaataat tttcaaaaat ccgactttaa atagaagaag tattggaaag tttttagagc
      121 tatgctacta ttatgaagtt gtaggaggag aaaattatga tcgaactcat aaccacagta
      181 gaaactattg aacaagcaga acagttgctt gctgcaggtg cagatacact atatttcggt
      241 gaagagcaat ttggattgcg tctgcctgct tcatttacac gagaagaaca gcgacaattg
      301 gtagaattag cacacaagta tggtaaaaaa gcgaatattg ctgtaaatgg gatcattcat
      361 cctgaaaaaa tgaaaaatgt acctgaatat ttggcttttt taaaagaaat aaacgtagat
      421 gtgatcacag ttggtgatac aggtattatt tatacgatga aaaaaaatcc tgaattgcgt
      481 ataccgtata tttatgatgc tgaaacatta gtaacgagcg cacgtcaaat caatttctgg
      541 gcgaaaaaag gcgcagttgg agcagtactt gctcgtgaag ttccttttga agagatgaag
      601 gcgatggaag aaaaactcga tattcctgta gaaacccttg tgtatggtgc tacatgcatc
      661 catcaatcca aacgtccact gctacagaat tactataact atacgaaaca agacgaacaa
      721 aaagatcgtg aaagaggtct ttttatctcg gaacctaaaa aagaagagac acattattct
      781 atttatgaag atagtcatgg cacacatatt tttgccagca atgatctgaa tctctcgaat
      841 gaattgaatg aactttatca gcatcattac tgtacatgga aattggacgg attatttact
      901 ccaggcgaaa aattcgtaaa catcactcga ctattcgccg aagcaaaaaa acaaatttta
      961 gaaggtagct gggatcaaga aaaagcggaa aaaatgacta gcgaaatcag taaattgcat
     1021 cctgaaaacc gtgaattaga tacaggattc ttttatattg atccttctgc tatcaaataa
     1081 ggagtatgat gaaagaaatg acagtaaaaa gaaaattgaa acgaccagaa atcctagcgc
     1141 cagcaggaac actggaaaag ttaaaaaccg cgatccatta tggagcagat gcagtatata
     1201 ttggcgggaa tgcatatggt ttaagaagcc gtgcaggtaa ttttacttat gaagaaatgg
     1261 ctgaaggagt tgcttttgca aaagaacatg atgcgaaggt atacgttgct gctaatatgg
     1321 tcacccatga aggaaatgaa gatggagcag gggaattttt ccgcagtttg cgtgatgtcg
     1381 gtatttctgc agtgattgtt tctgatccag ctttgatcga aatttgtgcg acagaagccc
     1441 caggtctgcc gatccattta tccactcaag cttcagcaac taattttgag acattggaat
     1501 tctggaaaga agaagggttg gaacgtgtcg ttttagctcg tgaagtttca atggaggaag
     1561 tggcagagat tcgtaaaaat acggataccg aaatcgaagc atttatccat ggtgccatgt
     1621 gtatctctta ttctggtcga tgcacattgt ccaaccatat gtcgatgcgg gatgcaaacc
     1681 gtggcggatg ttctcaatct tgtcgctgga agtatggatt gttcgatatg ccttttggtc
     1741 aagaacgaaa cagtttaact gatacaggcg aagtggaaga agaattctcg atgagtgcag
     1801 tggatatgtc aatgatcgaa catattccag aattgattga aaacggcgta gacagcttca
     1861 agatcgaagg aagaatgaaa tcgatccatt atgtatctac ggtgtcaaat gtctataaag
     1921 cggctgtaga cagttatatg gaagatcctg aaaactatgt ctgcaagcaa gaatggatcg
     1981 acgagttatg gaaagtagca caacgagaat tagctacagg tttttactac aatacaccaa
     2041 ctgaaaacga acaattattc ggtgaacgtc gtaaaatccc agtatataaa tttgtcggtg
     2101 aagtattatc ttatgatgag gcaacacaga ttgctacgat acgtcagaga aacttgtttt
     2161 ctgtaggaga tgaaatcgaa ttttatggtc caggattcag tcatttcagt caaaccgttg
     2221 aagttatgca taatgaagaa ggcgaatcca ttgaccgagc accaaatcca atgatgattt
     2281 taactatgcc agtcgcacag ccggtgaaac ctggagatat gatccgaaaa cgtaaataag
     2341 gaaaaagctg atatatccct gttttaagca tcttcgcata caaaattcaa gaaaagtgtt
     2401 acacttgttt tctgtagaaa aaggatcgtt aaataaaggt tttgattgat ctcttcaata
     2461 aggaggaaca aaatgcaaat atatgattac atcgtaattg gtggcggctc tggcggtatt
     2521 gcttcagcaa acagagcagg gatgcatggc gcaaaagttt tgctgattga agcgaatgaa
     2581 ttgggcggaa catgtgtcaa cgtcggttgt gtacctaaaa aagttatgtg gcaagcaagt
     2641 acgattttgg aaacaattga acgagatgcc tcaagttacg gtatccaagc agatttaaaa
     2701 aatgttgatt tcgccggtct agtagaaaga cgagaaaaat atattgattt tctacacggt
     2761 gcttatcaaa gaggattaga tagcaatcat gtcgaggcta taaaaggtta cgctactttt
     2821 atcgatagtc aaacgatcga agtaaacggt gaaacctatc gtgctcctaa aatcttgatc
     2881 gctactggag gccgtgcaag taaaatgacc attcctggtg gagaatacgc aattgattca
     2941 aatggatttt ttgaattaac agagttacct aagagagcaa tcgttttagg tgctggatac
     3001 attgcggctg aactttcagg tgtatttcgt ggtttaggta gtgaagtcat gtgggcatat
     3061 cgaaaagaac gaccacttag aacgtttgat aaaatgcttt cggacaattt gatccaaatg
     3121 taccaagaag ctggaataaa aacttacgca catcatattg ctaaagaaat tactaaaaac
     3181 aatgacgaat atacggtgct atttgaaaat ggtgaaacct taactggtga ttgtgtactt
     3241 tttgccggtg gacgtgtacc gaataccgaa aaattaggtc tggaaaatac aaatgtggaa
     3301 ttggacaaaa aagggtttat caaagtagat aaataccaaa atacaacgga tgaacatatt
     3361 tttgctgttg gtgatgtgat cggtaaatta gatctgactc ctgttgcaat cgcagctggt
     3421 agacgtttat cagaacgttt atttaatgga aagaaagatt cttatcttga ctataaactt
     3481 gttccgacag tcgtctttac gcatccacca attgcaacaa ttggtttgac agaagaagaa
     3541 gcattggaaa aatatggcga aaatgaattg aaagtttatc gttcacgctt taccccaatg
     3601 tactttgcat taaatgatta tcgccaaaaa tgcgaaatga aattgatctg tgtcggaaaa
     3661 gaagaaagaa tcgtcggctt acatgctatt ggtattggtg tcgatgagat gctgcaaggt
     3721 tttgccgtgg ctatcaagat gggtgcaact aaagaagact ttgacaatac agtagccatc
     3781 catccaacag gagcggaaga attcgtaacg atgcgataat ttaatattaa cagttccaac
     3841 agctttttat tgaacaaacc atgtaaaacc agaaaatcag tatactgatt ttctggtttt
     3901 ttgtttttta gttatttatg taagattgct tcgataattc tcaaaacgaa ataagtaggg
     3961 aagttggaga ttttgattat cttcgtaact gacgaagagt aaatgaatcc atttggtatg
     4021 cactcttaag ttcaaaatat tggtatcgat gtttaaaaga aatagaaatg tatcgaagaa
     4081 cagatgttta aaaaagtgtg actttgtata aataaattag aagaagcaaa cgaggatgtt
     4141 aaaagaatac catatagtta ccaaaccaag aaagttctcg tcaagttcgc tggtacgaat
     4201 caaatcaagt gaaaaaaggg actgtgaaaa aaattgtgtc atagttagtc actttttccg
     4261 aacaaatagt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
     4321 gaactatttt tccatttgtt tctctaatac tcaggacaaa acactgattt tacaacctct
     4381 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
     4441 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     4501 ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
     4561 taaggagcta tttttatttg gctgttctta ttacttgatg cagggcagtt ttttcacaac
     4621 ctcttcatgc tgttaaatgg cttgcataat gattgttagt aatagcccca atacataact
     4681 ggcattgaat aagaccatat ttttgatgga atagctgaaa ctgataggct ggggtaacgt
     4741 ttgcttgaat tgatccaaat gcttcttcac aattggcaag gtcaagaagg tgaccaagat
     4801 cggccattca tagataccgc tcaacaaccc cattaaaatc acgaaatagc aagcatacat
     4861 caatagctgg aataagactg ttcccgcagg acgtccgata taaaaaacta gtgtatagcg
     4921 atgattttca atatcacggt caagatcccg caaattatta gccaacatga tattggcaat
     4981 cgtaaacacc aagggtaagg aagctaatac gattgctgct atagcccaaa gatttcctac
     5041 caaagcaaat tgtccagatg aaaagttgac tgtcagatca tagacatctg aaccgaatgt
     5101 attgacataa acgaccatgg caaaaattcc aaggcccata gtcaccccac tgaaaatttc
     5161 ccctaaaggc attcgagata aaggaatcgg tccgaatgta tagaatactc caataaaaca
     5221 acagataaat cccatcaaaa gtaaaagcca acccgtattg atcgttagaa taacgccgat
     5281 aacaaaaaca aatgcaatca tagctaaaat catgtttcga atcaatacag gagatagatt
     5341 tctgcggcca atgacatttt cttcatactt atatacttca gaatgagcct tttcataatc
     5401 catgtaatta ttgatagcgg tagtcgctaa atcaaaaact agcattccaa taaaaaataa
     5461 aaaagtgttt agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc
     5521 aaaaggaaat acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag
     5581 tgtcatggtt cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc
     5641 ctccgataat ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt
     5701 cacgaagatc gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt
     5761 cacacccatt gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat
     5821 gatcgtgaca cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg
     5881 atggtatttt ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt
     5941 tttgttccgt tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat
     6001 gccgactgtc cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc
     6061 gcccaagtca acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc
     6121 cgtaatgtaa cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata
     6181 aacagttttt tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg
     6241 atcgatttct tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt
     6301 gattgcacca atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg
     6361 cttgactaac gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt
     6421 cacttcatcg atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac
     6481 tcgatcgaag gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac
     6541 gtaagtccct aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc
     6601 tgaactttca gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga
     6661 gatgcccagt gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt
     6721 ttgaaaggtg agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta
     6781 tgattatgtg attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct
     6841 ttttcagctg cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta
     6901 gcacctgtaa caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg
     6961 aattcgtcgt tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata
     7021 ttttcattat attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag
     7081 tttgattcag ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta
     7141 gagtagttct tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt
     7201 tcgattgtat ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg
     7261 aagctttcag atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct
     7321 tttaagaatt ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct
     7381 tccatgtttt tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta
     7441 tcatattttg attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa
     7501 ccatttgaat aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct
     7561 acaacttttg cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat
     7621 tcagaagaag agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct
     7681 gaaaatgcta atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac
     7741 cattccattt ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag
     7801 cctataagtc aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag
     7861 caaatttcgt gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac
     7921 aaaagaacag aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga
     7981 attatcatat attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa
     8041 aatattgtcg ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag
     8101 aaactgaaaa aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg
     8161 atgaccgagt tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat
     8221 ctgcagcatt tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt
     8281 atcgataaag aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg
     8341 atccttggaa tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga
     8401 ttcacccttt ggtcatttga agattctgta aggattcggg aacatattga gaagacagta
     8461 gcaaaagctg cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc
     8521 tgtggttctg gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc
     8581 ctagccaaag attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg
     8641 ccgacgatct tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt
     8701 aagcacaacg tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc
     8761 aaattgaaag acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa
     8821 ggaacttctg acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc
     8881 aatgaatata tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta
     8941 gtcttttatg aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa
     9001 acaggacaca cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa
     9061 tttaaaggaa actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac
     9121 ctatttgata agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat
     9181 ttgaaatact tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag
     9241 tttttccata tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat
     9301 gtcttatgga gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc
     9361 aaaaaaatca ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca
     9421 tggttcactg gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc
     9481 agtggtgctt ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc
     9541 ggagaagcag cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca
     9601 atggctgtga ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca
     9661 aacaatgatg tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca
     9721 ttagcattga ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg
     9781 atcacgttct gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct
     9841 ttcgcattga tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg
     9901 ctacaacgta cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa
     9961 taaatagata aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac
    10021 cgtcacagcc tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat
    10081 gagcaagaca caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga
    10141 agtagcttct tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa
    10201 attccttctt atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt
    10261 tccacctgct gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc
    10321 tattcttcgt tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc
    10381 taaaattggg tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct
    10441 gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct
    10501 tttttaccag tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg
    10561 gtactgcgtg tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc
    10621 aacacatata cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat
    10681 cgagtacgga taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca
    10741 acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca
    10801 acagatggag gtgacgaaga cagtctgatt tattagacct tcacacacca tgagtaaatt
    10861 acaaaaaatg atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga
    10921 aaatatgatc ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct
    10981 aatcacgatc gttgccattt ttaccatgaa aaaacgagac agcttcttac tactttggct
    11041 acgactattc ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag
    11101 tggtgctttg cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt
    11161 cagtatcatc ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat
    11221 ggcttctttc attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt
    11281 agggatttta tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca
    11341 gacacttcaa cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat
    11401 gtttaccaaa aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg
    11461 aaaactatcc tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg
    11521 tccgcctgaa aaacaaacct gtcgaacaag caatcaaaga aacgatccat gccggcggaa
    11581 agctgttgcg tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag
    11641 acaaagccat cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg
    11701 atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg
    11761 gtaatgtcgt ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt
    11821 ctgattactc cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat
    11881 tgaatggcga attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat
    11941 atttggaaaa tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg
    12001 cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta
    12061 tcgggatggc ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa
    12121 tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact
    12181 ctttgcaaaa aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag
    12241 aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta
    12301 ggaagttagc ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca
    12361 cctctcttcg aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag
    12421 actgaatagg gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt
    12481 ttgacaaatt gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt
    12541 ccgttagcga cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata
    12601 actcataaaa gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca
    12661 attattcgat tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt
    12721 gcttgtttta ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga
    12781 gacacttgca tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt
    12841 tgatgaatct gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga
    12901 cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggtagaggt
    12961 agtatctgta ctgtcacttg aagctgacgt gtccgtgcta tcacttgttg tggtagatgt
    13021 atccgtactg tcgcttgaag ctgacgtgtc cgtgct
//
LOCUS       DAYYAJ010000086        13002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000086 DAYYAJ010000000
VERSION     DAYYAJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.086"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..654)
                     /locus_tag="ACHATZ_002005"
     CDS             complement(19..654)
                     /locus_tag="ACHATZ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8404525.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(801..2021)
                     /gene="sstT"
                     /locus_tag="ACHATZ_002006"
     CDS             complement(801..2021)
                     /gene="sstT"
                     /locus_tag="ACHATZ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8404526.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            2240..3238
                     /locus_tag="ACHATZ_002007"
     CDS             2240..3238
                     /locus_tag="ACHATZ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404527.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            3434..5269
                     /locus_tag="ACHATZ_002008"
     CDS             3434..5269
                     /locus_tag="ACHATZ_002008"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8404528.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(5329..6045)
                     /locus_tag="ACHATZ_002009"
     CDS             complement(5329..6045)
                     /locus_tag="ACHATZ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8404529.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(6272..6814)
                     /locus_tag="ACHATZ_002010"
     CDS             complement(6272..6814)
                     /locus_tag="ACHATZ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8404530.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(6819..7046)
                     /locus_tag="ACHATZ_002011"
     CDS             complement(6819..7046)
                     /locus_tag="ACHATZ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8404531.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(7125..9005)
                     /locus_tag="ACHATZ_002012"
     CDS             complement(7125..9005)
                     /locus_tag="ACHATZ_002012"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8404532.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(9194..9808)
                     /locus_tag="ACHATZ_002013"
     CDS             complement(9194..9808)
                     /locus_tag="ACHATZ_002013"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404533.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            9950..10510
                     /locus_tag="ACHATZ_002014"
     CDS             9950..10510
                     /locus_tag="ACHATZ_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8404534.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(10815..11129)
                     /locus_tag="ACHATZ_002015"
                     /pseudo
     CDS             complement(10815..11129)
                     /locus_tag="ACHATZ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(11383..13000)
                     /locus_tag="ACHATZ_002016"
                     /pseudo
     CDS             complement(11383..13000)
                     /locus_tag="ACHATZ_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4155 a         2531 c         2083 g         4233 t
ORIGIN      
        1 aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaaccagg caatgggcac
       61 ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta atggccaaga
      121 atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac tgatcacaga
      181 cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga tcaagaaaag
      241 aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat tatggaccca
      301 tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg taaagataca
      361 atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca cacgagatgt
      421 actaacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc ctgaaaccac
      481 agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata cactgatcac
      541 gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag tcaaaaaatc
      601 actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat ccatgatacg
      661 cctccaataa caattaccta tgcttttaat tgttattata aacgacttta tcttgattta
      721 acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga atcattttaa
      781 aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc tttcaatttg
      841 attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa caacagatca
      901 cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat aaatccaata
      961 ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc taatggaatc
     1021 aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc agacaaaata
     1081 cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac agcagtcaac
     1141 gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc taacgggatc
     1201 gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat gtttatcggg
     1261 atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt caaacaaaag
     1321 aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg gtacacaact
     1381 aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata ctctgccaat
     1441 ccagcgattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc tatcggagca
     1501 aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc caccaactct
     1561 tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca gaataagacg
     1621 ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt cgtcaacata
     1681 gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc ttgtaaagta
     1741 ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag caatgttccc
     1801 actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt atgcttggca
     1861 attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa gcaactgaca
     1921 aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc aaacaatatt
     1981 ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa cttctctaac
     2041 aaatgaccac cctttcacaa ttttaacaat cattgtatca ttttatgcaa agaaactaaa
     2101 ggtataattt tctaaaaatt ctaacaatac aaacaaccat ttcttgctaa ttttgtactt
     2161 tttctgtcta cacgttgttt tttgagatat tctaagttag gatgttatta atgggcaact
     2221 tatcaggaaa cgttttctta tgtttttgtc caattttttt gtgaggtgtt tattcttgaa
     2281 aaaaataaag aaggagagtt atggcttcct gacaatcttt gtcgcttgta taatgatggt
     2341 tttacagcgt atattgattt cagcagtcac tattcctaca tatgcaacaa accctggtcg
     2401 attctacatc tatacatttt tacaaacttc cgttgttatt ggtgcaaatt tgatcccact
     2461 ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa gttttacatt atttaagtcg
     2521 ttttgccttt atattcctaa tagccacact agtttgcaat atcttttttt acgtacaagc
     2581 tggcagcttg aatattcgtg attattggct tatcctaaca cctatcagtc agaattactt
     2641 tacttatgct gtttcttgta ctttgctttt ttgtagtatt ccttatacag ttggattttt
     2701 aaataaatta tcgaaaacaa caattaaata tctactatta tttttaacaa ttggattagt
     2761 tggctgctct actctattta ataaagaccc gtttgcatta cagaatggac agagtatttt
     2821 atggattttt tatttatttt taatgggtta tggtttgaaa gaatggaact ggaaagaaaa
     2881 ggtccgctat aagcttcctc aagtatttat cagcttgatt gttttattcg gtttgattat
     2941 tttgatgaca caaatttcac tcatcatcag aggaaataca gatacagcgc tgaggttttc
     3001 taaaccatat tctctcttcg caatgtatta tactttctct tcgtttttat ttttagaaat
     3061 gctatctgat aaattaggta taaaaggaag aggaaatttt cttgctgtat ttttgattgt
     3121 cactcaagtt atatgtaatt tccctttagt ttcttatcgc gtatctacat ttatgaaacg
     3181 tgaatttcca aactcaggga aggcatggct gataaatatc gggcagtttt ttatttgata
     3241 tttattagca gtgtccctcc tagttatctt tttattttat atacaaaaaa tccgtttcat
     3301 tcatcgtttc atcgaatact tcgcttttga ttcgattgaa caattaactc agcgttggaa
     3361 aaaaagaatt cattggcttt ttgttcataa aagttatttt ttagttgcag cattttttta
     3421 ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa gaaggttgga aagaaactat
     3481 ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc atgatcctga caatcatcat
     3541 tattatggga tttttcttct tactattgtt gatttccaat cgtttttggt acgccttgac
     3601 tgctaccttg atcattaact tactgctcac tatttcgaca gtaatcaaaa tggaaatgcg
     3661 ggaagagcct gtctttcctt cagatttaaa aatgctgacc ggattgtcag aactattatc
     3721 gatggttagc ccagtcttat taatcgtggg cggattgatc cttctcctat tattgattac
     3781 tagtattatt gtacaacgta gactacaaaa acagtattcc ttgaagattc attggaaacg
     3841 tcgatttatc agtatcgctg tattattagg atgtttttcg ggtgtctttt tcattaatca
     3901 caaaaattcg ccttctttct tgctgtttaa tttatttaaa gtaaacaaga ctttctttaa
     3961 ccagcaaggc gccgtaaaag ataacggacc agtcattcaa ttcctaaaca atatcgacat
     4021 taaaatcatg gaagaacctg ctggctattc gaaagaaaaa attaatggaa taatgaaaaa
     4081 gtacaacaca aaggcagatg aaatcaacca aacaagaagt gactggttaa acaatcagac
     4141 gatcgttctc aatttaagtg aaagcttcag tgatcctgca cgtgtcccta atctgacagt
     4201 acctacagac cctattcctt acatcaaaag cattataaca gaaggaacag gaggattgat
     4261 gttatccgtc ggttatggtg gcggaacagc taacatggaa tgggaaggac tgacaggctt
     4321 gagtatcagt aacctgtctg cttcgttagt tacaccttat acccagctag tggagcgtca
     4381 gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa atagccatcc atccttttac
     4441 agctgctctt tatcgcagaa aacaagtgtt tgataaattt ggttttgaca ggttctatta
     4501 tttagacagt ccggataaat taacttatac ggataaaata gaacaaagcc cacgcatatc
     4561 ggatgattca gcatacaaag aaaccttgaa agctttacaa agcaatacgg aaacgtcaca
     4621 attcatccaa ttatccacta tgcaaaacca tatgccttat gatgattact atagtgaatt
     4681 gaactacact gctgaaggaa ctgctgtcat tcctaataga agaaaagaat tacaaactta
     4741 tatgcaaggc cttcactata ccgatacagc agtaaaaaaa ttcatccaag aaatcgacaa
     4801 aatcgataaa ccgattacct ttgtttttta tggtgaccat ttacccgcaa tctatagtgg
     4861 gaatgattcg aaaaaatatg gattagaaca gcaccagaca gattatttca tttataacaa
     4921 caagtatagt cgtgaacaag cagctacgtt aaataaagaa gttgttgctc ctaataactt
     4981 tggtgctatg gctcttgagc atgcgaatat caaagtaact ccttactatg cgttgttgac
     5041 agaagtatct aatcatctac ctgcattaac aattgatcct acagaaagct tgtctaatcg
     5101 ttttaatgga aaacaggtct ttgttacaga ggatagtcaa attatccaag aaaaaaattt
     5161 aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg atacaatatg atcttgttgc
     5221 aggtaaccag tacagtgcaa catgggcgac acaaaaaata aaagactaac aaataaactg
     5281 agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt aatttgcttg
     5341 tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct cgttgaagta
     5401 aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg gctgattttg
     5461 ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg catcggaata
     5521 gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt ctgcagaaac
     5581 aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag ggactttaag
     5641 accatataag tccacactca tttgattcgt ataacttttg tattgttgtc ccattgtttg
     5701 gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc acagtgtttg
     5761 ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag aacttgaagt
     5821 agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg tatctgctgt
     5881 ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag ttgcaactgt
     5941 tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg tacaaccagc
     6001 taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa aaactccttt
     6061 cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg atttttcgta
     6121 ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga tgttgtctgt
     6181 aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt tacctcacgc
     6241 tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc tcatccaaat
     6301 cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct ttattgaaac
     6361 ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca gctaatttga
     6421 ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt tccaacattg
     6481 ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa cgcatgactt
     6541 cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag attttattga
     6601 accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct tgcgaagatg
     6661 tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta aaatttgcta
     6721 atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct gcttgttttg
     6781 tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt tacttttgct
     6841 gattggactt cataacctaa tttttccaca actgcagcta attgttcagg gtggacttca
     6901 tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt attgcttacc
     6961 ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca agctagtgta
     7021 tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt gatagtctta
     7081 ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga tttttcttct
     7141 ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat caagatacta
     7201 gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa gccgttgagt
     7261 aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa aacagttttc
     7321 ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa gacaacgtca
     7381 ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc agccgcagcc
     7441 aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc ttgttcttgg
     7501 tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac ttcatcaatg
     7561 cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag catcactaac
     7621 tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg tcgaatcaca
     7681 tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat gaccgagctg
     7741 cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga aagtttgtga
     7801 gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc agctgtgatt
     7861 ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc aatgtctttc
     7921 tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt ttctaatcga
     7981 gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt ggatacagca
     8041 gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat tttggaaaaa
     8101 gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt accgattcct
     8161 gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag aaccaaaaca
     8221 gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat aagtaaaacg
     8281 gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga tttggtatct
     8341 tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc gaccttagtt
     8401 gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc atctccactg
     8461 attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata ccctttcccg
     8521 tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac gatatcgcct
     8581 tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac aacactagca
     8641 gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt caatgttttt
     8701 tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc cttgtattct
     8761 ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga gataactttg
     8821 actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat agaagctatg
     8881 gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa accaaaaata
     8941 atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca cttttgtaat
     9001 ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat acttatccat
     9061 cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt ggtagaatac
     9121 actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac tattgtcgtt
     9181 tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga tctatttctt
     9241 tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca cgtcgttcat
     9301 agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct ttctctcttt
     9361 tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca ggatttgcag
     9421 ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca agcatttgtt
     9481 catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt aaaaaatcga
     9541 atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct ccttttatct
     9601 ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt tgagttgctc
     9661 gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt tcattttcca
     9721 tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg taatctttct
     9781 tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa tgattgtgtt
     9841 atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct ataagtttgt
     9901 cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa tgaaaaagac
     9961 agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca cattattagt
    10021 aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag atgtacatcg
    10081 ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat tagaaagcaa
    10141 cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct atgaggaact
    10201 atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct tatttatcct
    10261 caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag aaaactatct
    10321 gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga gattctttac
    10381 tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa gcaattacaa
    10441 gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca tagagaaaat
    10501 catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct tctcgtataa
    10561 caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa atttctataa
    10621 ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt gatatatctt
    10681 gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa tttattacaa
    10741 cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca tccagtttat
    10801 tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc atctaacatt
    10861 ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc tggatgtttg
    10921 gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg aattggatct
    10981 tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac aaaccactca
    11041 ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg tttaatgaca
    11101 aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat tcaaagataa
    11161 ttttctcaat aaagattttt gattccagta agattccttt actcctttta caacatagcg
    11221 ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag cccaattctc
    11281 tataaagaag attaagcttc attattataa tataaagttt aaatttggtt tattacttac
    11341 tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca ttatttttat
    11401 atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac ataaactaat
    11461 taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga ttgtttctcc
    11521 ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt ccatttactt
    11581 ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct ttagattcat
    11641 gtactgtata gactattttc gaatccatca cttgataagg catagtatgc cattccttgc
    11701 caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc gtgggtgcat
    11761 agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct tgattcatta
    11821 ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt gcagcagata
    11881 cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc ttagctgatg
    11941 tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga aagcctaaga
    12001 ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac catggaaaaa
    12061 atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt tctttgcgat
    12121 taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg attcctaccg
    12181 taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca tttaatgctt
    12241 ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt tgtcggaaca
    12301 tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt gacaactcca
    12361 caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg cctaagacta
    12421 gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa atgagtaagc
    12481 tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt gcgtaagcat
    12541 aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca gaacgatgat
    12601 aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata aaagccgcat
    12661 cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta caaacattca
    12721 atagccaaac atataagata taactaacga ttaaattatt acttatccaa taaaaaatga
    12781 ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca taatgattaa
    12841 acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact ctagcaaaat
    12901 ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa ttgattgaat
    12961 aaagtccagc aataattgct aggagaatgg acaatattat tt
//
LOCUS       DAYYAJ010000087        12930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000087 DAYYAJ010000000
VERSION     DAYYAJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.087"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHATZ_002017"
     CDS             414..947
                     /locus_tag="ACHATZ_002017"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8404535.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="ACHATZ_002018"
     CDS             complement(1437..1961)
                     /locus_tag="ACHATZ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8404536.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACHATZ_002019"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACHATZ_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8404537.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACHATZ_002020"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACHATZ_002020"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8404538.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="ACHATZ_002021"
     CDS             complement(3683..4363)
                     /locus_tag="ACHATZ_002021"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8404539.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="ACHATZ_002022"
     CDS             4489..5358
                     /locus_tag="ACHATZ_002022"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8404540.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="ACHATZ_002023"
     CDS             5384..5899
                     /locus_tag="ACHATZ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8404541.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="ACHATZ_002024"
     CDS             complement(5946..6509)
                     /locus_tag="ACHATZ_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404542.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="ACHATZ_002025"
     CDS             complement(6564..6743)
                     /locus_tag="ACHATZ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404543.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="ACHATZ_002026"
     CDS             complement(6820..7329)
                     /locus_tag="ACHATZ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404544.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="ACHATZ_002027"
     CDS             complement(7425..7805)
                     /locus_tag="ACHATZ_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8404545.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /gene="pnuC"
                     /locus_tag="ACHATZ_002028"
     CDS             8135..8884
                     /gene="pnuC"
                     /locus_tag="ACHATZ_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8404546.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="ACHATZ_002029"
     CDS             complement(9068..10345)
                     /locus_tag="ACHATZ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404547.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACHATZ_002030"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACHATZ_002030"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8404548.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="ACHATZ_002031"
     CDS             complement(11267..12211)
                     /locus_tag="ACHATZ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8404549.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="ACHATZ_002032"
     CDS             complement(12213..12899)
                     /locus_tag="ACHATZ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8404550.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3726 a         2707 c         2295 g         4202 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt
//
LOCUS       DAYYAJ010000088        12926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000088 DAYYAJ010000000
VERSION     DAYYAJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.088"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..565)
                     /locus_tag="ACHATZ_002033"
     CDS             complement(98..565)
                     /locus_tag="ACHATZ_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8404551.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            873..1124
                     /locus_tag="ACHATZ_002034"
     CDS             873..1124
                     /locus_tag="ACHATZ_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404552.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            complement(1157..1444)
                     /locus_tag="ACHATZ_002035"
     CDS             complement(1157..1444)
                     /locus_tag="ACHATZ_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404553.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(1441..1650)
                     /locus_tag="ACHATZ_002036"
     CDS             complement(1441..1650)
                     /locus_tag="ACHATZ_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404554.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(1647..1844)
                     /locus_tag="ACHATZ_002037"
     CDS             complement(1647..1844)
                     /locus_tag="ACHATZ_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404555.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(1841..2167)
                     /locus_tag="ACHATZ_002038"
     CDS             complement(1841..2167)
                     /locus_tag="ACHATZ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404556.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(2164..2445)
                     /locus_tag="ACHATZ_002039"
     CDS             complement(2164..2445)
                     /locus_tag="ACHATZ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404557.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(2458..2676)
                     /locus_tag="ACHATZ_002040"
     CDS             complement(2458..2676)
                     /locus_tag="ACHATZ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFM8404558.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(2673..2984)
                     /locus_tag="ACHATZ_002041"
     CDS             complement(2673..2984)
                     /locus_tag="ACHATZ_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8404559.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(3161..3475)
                     /locus_tag="ACHATZ_002042"
     CDS             complement(3161..3475)
                     /locus_tag="ACHATZ_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8404560.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(3475..3774)
                     /locus_tag="ACHATZ_002043"
     CDS             complement(3475..3774)
                     /locus_tag="ACHATZ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404561.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(3777..3938)
                     /locus_tag="ACHATZ_002044"
     CDS             complement(3777..3938)
                     /locus_tag="ACHATZ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8404562.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(3953..4312)
                     /locus_tag="ACHATZ_002045"
     CDS             complement(3953..4312)
                     /locus_tag="ACHATZ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8404563.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(4309..5160)
                     /locus_tag="ACHATZ_002046"
     CDS             complement(4309..5160)
                     /locus_tag="ACHATZ_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8404564.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(5177..6010)
                     /locus_tag="ACHATZ_002047"
     CDS             complement(5177..6010)
                     /locus_tag="ACHATZ_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8404565.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(6017..6703)
                     /locus_tag="ACHATZ_002048"
     CDS             complement(6017..6703)
                     /locus_tag="ACHATZ_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8404566.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(6709..7455)
                     /locus_tag="ACHATZ_002049"
     CDS             complement(6709..7455)
                     /locus_tag="ACHATZ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8404567.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(7448..7789)
                     /locus_tag="ACHATZ_002050"
     CDS             complement(7448..7789)
                     /locus_tag="ACHATZ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404568.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(7847..7975)
                     /locus_tag="ACHATZ_002051"
     CDS             complement(7847..7975)
                     /locus_tag="ACHATZ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404569.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(7968..8675)
                     /locus_tag="ACHATZ_002052"
     CDS             complement(7968..8675)
                     /locus_tag="ACHATZ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFM8404570.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            8720..9199
                     /locus_tag="ACHATZ_002053"
     CDS             8720..9199
                     /locus_tag="ACHATZ_002053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404571.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(9258..9428)
                     /locus_tag="ACHATZ_002054"
     CDS             complement(9258..9428)
                     /locus_tag="ACHATZ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404572.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(9440..9706)
                     /locus_tag="ACHATZ_002055"
     CDS             complement(9440..9706)
                     /locus_tag="ACHATZ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404573.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(9717..9908)
                     /locus_tag="ACHATZ_002056"
     CDS             complement(9717..9908)
                     /locus_tag="ACHATZ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404574.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            10199..10621
                     /locus_tag="ACHATZ_002057"
     CDS             10199..10621
                     /locus_tag="ACHATZ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8404575.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            10638..11060
                     /locus_tag="ACHATZ_002058"
     CDS             10638..11060
                     /locus_tag="ACHATZ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8404576.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            11089..11289
                     /locus_tag="ACHATZ_002059"
     CDS             11089..11289
                     /locus_tag="ACHATZ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404577.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            11376..11825
                     /locus_tag="ACHATZ_002060"
     CDS             11376..11825
                     /locus_tag="ACHATZ_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFM8404578.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            12127..12264
                     /locus_tag="ACHATZ_002061"
     CDS             12127..12264
                     /locus_tag="ACHATZ_002061"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8404579.1"
                     /translation="MTLQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRV
                     K"
     gene            12402..>12926
                     /locus_tag="ACHATZ_002062"
     CDS             12402..>12926
                     /locus_tag="ACHATZ_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM8404580.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGE"
BASE COUNT         3824 a         2563 c         2018 g         4521 t
ORIGIN      
        1 cataataaat catttaccag gtaagtgatt ggtataaaaa aggtataaaa tggaaaccaa
       61 atgggtaatc aaagggtata aaaagtgtaa aaactggcta cttaaaagca accagttcta
      121 acgatgaagc aaattggatg ataattctgt ttgattctac tttaaccgat tcttcgctag
      181 tattattcct ttgagcagtt acataaatgg gcagaccatt gatataacga tcatagaata
      241 tcttcttgcg cctttctgtt acatcaggtt tatgcggatg ctgaattgct gaatagccac
      301 gaacaaataa tttgtgcaag tactcaaatt cttcttgcgc ttcctctctc tcaatcatca
      361 ttcgttctgc ttcaaatgta ttatttgctg tagaaggtgg aaccaaggag taagatgccg
      421 ttactttagg ctcccgaggt tgacctatcc tacatctagc tgacagatat gctgaaagaa
      481 aaacaccaac attatgtttt gtgcgatcca tatccacatc ttttgcctct ggtgtctcat
      541 acttctttac atcgaaaagt accatccatt gattcctcca tttatgatat aataattgtg
      601 tcagaattat taattaaggt cggaggaatc cggctttttt tatacccatg cttatgctaa
      661 gcttttcaag tagcgaggtt gcactcgtta cccatataca tgttgagcta tctggcggaa
      721 aacagatggc tcactatttc aatattctgc taaggacagc cagtggtcgg ctgtcttttt
      781 tatattttaa tgagaagcct tacttatttt tttatcttta ttacgaaaaa tgatattatc
      841 tattgataat aaagcgtata ggagatggtt gagtgactat gtggagcatg ctgttatttt
      901 ggattcccgt ttgtattggt atcgtcgcat tttgttactt tgtcaaacac tctagaacaa
      961 ataagctcct catgttatct tttttaccta tagtattttt tattgtacaa attgttaaat
     1021 atacctatat tgaatcgcaa gaaatattca ttttttatgt ggtaggttta tttatctctg
     1081 tggtcttttt cataatgata ctttcctatt tttataaaaa ataaattttt ctcttagaag
     1141 tacatttttt cgctgtttat atagccttca tatccaccaa tctcgctaca gctaaattct
     1201 ctttgctttt cgctaaccac ttatcacatt ccatcgtgtt ttcaatacga atgatcgctg
     1261 agtgattata gacgtgttct acatatccac gaaatggata gatgaaccct tctgcttcgc
     1321 agcgaaccat gtcaccgact ttgaattttg gtttcttacg ttttttagga ttctttgtcg
     1381 gcatatctag cattaaaccg ccgataccat gactactagc gtaaaatccg tcttttaatt
     1441 tcatctttct acctcctcat gatcgacagt gaccagttca tatacttgtg ttaatccacc
     1501 aagtcgtcgt gaaacttggt tagcttcttc gaggctatca aaccatctcg cactttggag
     1561 tatgtctaca atgaataatg tgttagcccc tatagagcgt tcatcacttc tataaaattt
     1621 attcccgaac tttactacat aaatcttcat tctgcttcct cctcaatcgt ccaagcattt
     1681 gtaaatttct ctaagcttat tagttcatct tcatttaaat atccgtaacc atataaggat
     1741 tctaaaaggt cttcttttga atagcctcta aaatcaagtc tgagaccgct taggatgtat
     1801 acataggttt tatttccata aaacttgatt gtgtatgctt tcatcccact tcctcctcat
     1861 cgatctctat ctcgacatct ctgccttcta aaatctgttt ggtatcaata taggctacga
     1921 gtatttttcg attttggctt aaagcaatat acggatcaat caaagcaact tctgcgtgta
     1981 gacgataata agcaatacaa tctatttcta aaaatatcaa acgatcaata gcatcattaa
     2041 tcataaaatc tgtaacgttt tctgctgtat ttaaaccatc tgcatattta ggcaggactt
     2101 cattattgat gaactcagct acatgttttg gacataaatc agtcagttct tgttgtgtat
     2161 agttcatttg ctgtcctcct gattaatata tttcctcaaa ctacgtatcc ctttaccacg
     2221 tttttttgtt ttgcgctcaa tccacgttcc tgtagttccc cagtagtcaa taattccatt
     2281 atgggtatta attgcaaatt gatgattgtt tggataatga gtaaattcaa atcccaaggt
     2341 ttcaatattt ttagttgctg aattgcccat ctttctgaca tgttgttttc tacgttcttt
     2401 tagatgcggt ttaatatctt tccagtattc gcccatatcg cccatttcgc tgtcctccta
     2461 tctccttgtc cacaataaaa accacatcaa ctcttctgtc gtaccgttta aataaagatt
     2521 tatttctgca ccaatcgcaa ctaaaattcc gacaacaaac aaaataatcc ctactgctgt
     2581 atattttttg ccttttgcac tctcgtaaat gaggtatgct cctaacatcg ctagtaagcc
     2641 gccaattaca caccaaaata ttgttaggct gatcatttgc tgtcctccaa atcactcgac
     2701 ttcacgaata caccatctac catttttcct gtgcgtcctt tgatttcgtt gtatgcttgg
     2761 ttcagacact cgtacaaatc catatcattt tgcatagcta aaataatcaa ggttactact
     2821 acatctccaa ttccgtctct taaaccatgt tcatcttttc tagctagaga agcggcaact
     2881 tccccgatct cttcgatcgt ttttaacatt tgcttgctgg aatcagcttg atccaatccc
     2941 ttatctttag cccactgctc tacttttgtg attagttcgt ccattattca ttctccttta
     3001 tatatttaag ttggataata caacttgtaa caaatgaagc taatgttaaa attgtgccga
     3061 ttggtgttaa aaaaccgctt tttatcgcat taattaatat agctacaaat atcgttatat
     3121 agaataaaaa gtgaattgac gctaacgttg aattaaacat ctagtcctcc tcgaaatact
     3181 cattcagtat ctctctatac ttttctacaa atttgaaacg atcctgatga agtttcttgc
     3241 tccaatttgt ttgccgatcc agctcacgca tctgatcgaa ccctttttga atttcgttgt
     3301 aataaaattc aatgtttgct gctgctttcc aatgcctgct acttcgcact cctgctcctg
     3361 tttcagccat ttccaactta actaattccg ctcgttcttt tgctttttta tctttctgaa
     3421 tcttcatcat gattttcttg aggatgatat cactgtattg tgtaatgaga tccattattt
     3481 ctcctccaca tacctaaact gtcgcccctt tgaatcaatc cataagctcc tagctctatc
     3541 ccagatgatg tttttgctca gtccagtaat ttcagataac tgttcagctg tacccgttac
     3601 tagaattcga tcaccatgcc agattgcaat ttttctcggc gttttccgtt taggcttttc
     3661 agtccacatt gatttaccga gcttttggac ttctgcaact atttctttgt cttcttgcca
     3721 attctcagaa tgtgtcagtt caatgattcg tttcattgct gctttcttac ccacgctcat
     3781 tcctccaatc tacgaatttc ccttcttaaa ttcactatgt gcaaattgat tgcctttctc
     3841 gccgtttcat tgaccatcac tgcctttatc cgttccagat cgtcaatctc acgttgaagg
     3901 cttcgaatac gcatttgaat cacttcttct gttgtcatga tggaccacct cgttaaaaac
     3961 gctcttcctt gaacgtattc cgatattttt tagctaatat caacggcact tgatattgat
     4021 gacagaacaa ctttgccttg atcttaaagt cttttgtctg cattcctttg acatctacga
     4081 ctttgacaag tttgccgttt ttataaaatg tgaagtcggg aatatactcg atcttgcgat
     4141 acttttttcc atctagttca aatttcggca tcagctcaaa tctttcctga agttttactt
     4201 tccagccgtt cgcttctgct tgccacaagg ctaaatcgta atactctgct tccgcgatag
     4261 aatcaaactt gatacctcga tgaacagttt tcttattacg gtatttattc atgcgatact
     4321 acctttcact ggttttatgc gcttgtctgc tgtttgttgg aatttcagcg cataaccttc
     4381 tgaattctta aatattctag aaacaattct ttcgccgtag gcttctctta gttcaggacc
     4441 ggataagttt gttgtgatga tcgttgcctt gttctgtctg gcttctaaga gcgtgtttaa
     4501 cgtgttgttt gtaaactgcc tactatttga taccccgcta cctaattcag ctccaatatc
     4561 gtcaaaaacc accaaatcag ttgttttgat atcggctata agcgatcctt caatttcttt
     4621 tctcagttca gcattgttat aagaaaactt tatttgctct aataactctt gatagcttat
     4681 aaaaagtatt ttcttgtcat aatttgagcg ctcaagtatt tcccaagctg tcgccattga
     4741 caagtggctt tttccgcttc ctgatttccc tgatagaatg aaatgtgcag gatggttcag
     4801 taagacatca tttacaaagc ttttagctct ttctaaagca attttcgttt cttggtccac
     4861 tacgtgataa ttctccattt tgcatttaaa caaagtttta tctgttaata ccgaaccatt
     4921 ttgaaaaaaa ctcaacgctc gtgcttttaa gctgtcgtta tatatccgtt cggtctgtat
     4981 atcctctttc acacgtaacg ctttataacc acaactcatg catgttggtt tacaacgttc
     5041 tgaaccatcc ttatttttag ctcgccaact atacaaaggt tcgctacatt ctggacattt
     5101 cccgctttgc actaatactc ttcttattag tttctccata gcatttgcta ggctttccat
     5161 gtgatgcatc tcctttttaa attggcaagt cgtcatattc actaggattg ctgtactgta
     5221 gtttttgact ttgcttttta tgattattct tgtctgcttt gatttcgaat ttgagcttct
     5281 caaatttttc tctcaatttc ttagcacttc taatatttcc aaaccaaaat tcatttgtag
     5341 gtagccaatt gatcacatac tcaatcgctt ctatagacgc tttatctctt tcttccatca
     5401 acctgattgt gtctgcccat ttttcgatat ctactttgtt catttctttt ggaaaatctt
     5461 cagttaaatt actttgcaat tttttagcaa ggcgtaagtg ttcgtcagaa tacttacctt
     5521 tcttttcttc tttatctata tctatatctt tctctatctc tatctctaac tctggtgtag
     5581 ttttgtctgg acatttgtcc gacacttgtc ctccagttat taaattccgt tttgcctctt
     5641 ctattttctt tctgtattct ctttttctat ctgcttcagt tgaggatttt ccaatgaaac
     5701 tttgtatatc agacatataa attgctccgt tatctaatac gtcaataagc tgcaaatcac
     5761 ggaaaatttg taccgctttt tctacgactc ctacagaatg tcttgtaata gttgcgagca
     5821 ttgtagagtt aaatggaatc ctgtcattaa acatcaactt accttcgtgt tttagacttc
     5881 ttaaataaag tttgagaaga atattagaat aaatatagcc atctggcata ctttctaaga
     5941 gaaccatctc gtcactatcg aaaaaattct cttttagttt taaatagtag tagcgtttgt
     6001 tgtcagacaa tattttttac cctcctattc taagtttctt aattgtttcc tggtttaact
     6061 tgatcccttt gatttgatat ttatttttga aattgatcac acctatattg tgtttctccg
     6121 tgtgatgaat cctgcagagt gctgcaaatg tgtactctga atgatcaact tctttgcgct
     6181 ttcgtcttcc tagcgctttg tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc
     6241 agcagactct ttttgtgatg catttgtaga agtaatattc ttgattcgct ggtaaaatct
     6301 catagccttc tttgaaagga atatgatgtt caaagatgaa atctaagatg atatttgcta
     6361 agacattagc atcactcaca gttgtattcg attcgtcttt gaggcttatt ttgcgccctg
     6421 tgacgccttc aaaacggaag taaaagaatt ccttccagaa gtccgttggc atgcctgtat
     6481 cgatgaaaat atcgcctatc agcgcataga tgaagtttcg ttgctgcaca gtaaatcgac
     6541 gtggatcaat aaatcgaact tcaataatcc gatcgccatc atatccgtca tacatcgtct
     6601 ttagtcgatc aatgttcact tcttcattga tcgttgcacc tatgtctttt cctttgaact
     6661 ttttcagaac cgctgaatat gaatcgatca atggtttaaa cactcatatc acttctcttt
     6721 tgtttcttct ctgtactgat cttcaatcca gttaacgcct cgttttagaa tgcccaagtc
     6781 tctcttggtc catttactgt catcagcggt tatagaagcc gcatcagtca atgcaacaat
     6841 tgcttcatca attgattttt cgtacttgtt agcaaccagt tgtaaagcat ccaagaatag
     6901 ctttttgctt ctttgagtag ctggttcaag catcgagaca tcttctggca tatcttcgcc
     6961 agcaaatata tatagcccta gcccaaacat tgctagattt tttacaagac agcgcatgat
     7021 cgttttgttg atatcaaaca tagttgctgc ttcaactcgc ttttcgattt ttccaacaat
     7081 ctcttttttc ttcgtttcgt tattccactg ataatcattg acttcgtagg tatatggctc
     7141 atctttcatt gccttgtttg caccatccat gactggtaac cacatgtcac gctttactcc
     7201 gttgactgtg atactggtaa aaaccatata gcctgttttc ttatcaaaga catacggcag
     7261 atatgtttct ggatcatgat agatttcata atctactgcc tcacacactt tgctaacttc
     7321 tgcccaagcc catgcccacg aaagataagt gaggttattt cgtttttcag taacatcttt
     7381 cacatttatt ttgtataagg tattaaaaag cgtattatca cttctatttt tcaaaggttg
     7441 ttccttttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta cctgatccgt
     7501 aataagagat atcaatcaag ttatctctgt catactcttc tagcgcatca atcaagccat
     7561 cttcgatgac atagatatat tcagtttttt tggacttcct cgataaatgg ataagataga
     7621 catgatccca aatactcaca aaatttccca aatcgtcttg atcacatgct agttcttcat
     7681 ccgtcaaaag attacgtctg atttttcgat tgcttgtttc cttgatattc gatttgcccc
     7741 aactaggatc agtcaaatat tgatctagag tggaaagttc ttttttcatg tggtaacatc
     7801 tccttagatg tattttcttt gtgactcatt gctttggtcg gctgagtcac ttttttattt
     7861 gttgccatgc tttttgctta tcaatatgtt gttggcttag gatgtttggt ttattgtgtc
     7921 tccaccagcg attagcaatt attacgccta tttttagcgc ttcagctcta ttcatcctct
     7981 aacaactcca tttgcttaat aattgtcatc gtcgctgtag aaggcatcca attgttaata
     8041 aaatcgataa catcatcgaa ctgttttgcc ttgatacgat ttctagctac tgctccagtg
     8101 attcgcttaa ttccgccatt caagtcttta aacaattctg cttttgcttt gctatttaaa
     8161 tgctgctgat tacaaataac tctaattttg ttgcgaacca tattagaaat tgttcggtaa
     8221 tctggttcag ctagaagttg attgttcttc aaatcgttta aatcagactc gatagcgtct
     8281 actctttcat tcgtttcttc gtttgcagct agagcaagta atgccatttc tcgtttagtt
     8341 gctggaattg atggcaattg gtcttttaat tctttttcca tcttgttgaa ggcctcaata
     8401 tattgaagtt taaaacttaa agctttctta ccagtgaagc ccattgctaa tagagagaag
     8461 ccgtctctat tcatataaat aattcggtat gattgtttat tttgaggatg aacataagta
     8521 tcttcccaaa ataggtctgc ccaattttcg gcaaccccct cttttagttc atcaatagcc
     8581 gtcaaaacat gtctgtgctt tttatcgaag ctttctgcaa cttgtaagct cgtagtcaca
     8641 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttgatata
     8701 attctggtgg ggtgaaaaca tgagcagaaa aattgataat ataatattgt taacaactat
     8761 tttttattta gtaatcggag caactctttc cttactgtca tttttgtcca acagtagttt
     8821 gattgtgtta aatcaaaaaa gcatattaac agaatttgcc cacatagatt acttctcttt
     8881 ttttaaaact ggactaatat gtttatttct atcttcattt atcctctata taggaactac
     8941 attttgtcta atggtgctta atttaaaaat gtttataatt gaaaaatgga tagttattat
     9001 cgcaaagatt ttctcatcga tatcaattat cattccaatg acattgacgt tttctaaaga
     9061 gcaatttaac gtagctacta cattcgtatc ttttatagct ctattttcgt ttgctgttcc
     9121 taaaactttt caaagcaaat ctagcaacgc aaaggttaat gtagaaaaca ctgatagaaa
     9181 aaatgatgaa agttattaaa aaaaataatt ctttcattta ttgtcaaacc tttcttatag
     9241 acattagact tttttagtca gtccctcccg actggctttt tcgctctgta ctcagcttca
     9301 tcaagcccaa tgaaaatcca aagcatgtaa acgatcgtgc cgattaatgc ttgtctacta
     9361 ccccatagac ctaaagcgta gataattagt ggcgcgctga atactaatgt tcgattaaac
     9421 tttcccatgt tgacctcctt taaattttgt atttagccat cagttcatcg atgtcctttt
     9481 tgtcatatct taaaaagcca tctatacgaa tttcttttaa accgtgagct attaacttct
     9541 caaaaccttg atcgtttaca cctcccacat attttctagc ttcgatcttt tttaaatatc
     9601 gtgtttctga caaattcttt tttgaaagtt gactaagtgc gtcctttaca acttcaacaa
     9661 ttgcaattct taaacgagtc aaaaactctt cgcttagaat attcatgact tgcctcctat
     9721 cgaattctgt aatcacggat gatctctaga atagttctgt ttgcctttgg tccactccag
     9781 tgtccatcaa gaatttgttg cattcgtaca cgcgtgtgac cataagcagt agccaaatca
     9841 tccatagtta ttccattctc tttcataaac tctttgatcg ccgcacgacc gttatccaag
     9901 tttgacatta acccacttcc tttttttata aatttgtaag ttaaaatgat agaaaaaata
     9961 taatttccat tgacattttt acactataga gtaaaatgaa tacatagaga aataagccta
    10021 taacaaaacc ttttatttgc acttggtcgc caaactttat gctataaggt gtgtttttag
    10081 tttgcttttt tctatcaaat taacttacgg ataaataata actctatatc gtaaaattgt
    10141 aaagctattt ttactctttt tcgtaaatta ttttttcgtg ttggaaagga acgttggaat
    10201 gacaacattt gaaagaataa aagaactagc gaaaaacaag aataaaaacc ttcaacaagt
    10261 cgctaaagat ttgaagtacg gagagaatta cttttatagt ttaaacgcag gaaaacagcc
    10321 aactgccgaa aagttacaag aaattgctga ctacttcgac gtatctgtag attatttgct
    10381 aggacgtact gagaaaaaaa gatactatga tttaacagaa aaagacgagc gtgatatcca
    10441 aaaagaattg cagtcattaa tcgacgattt atctaatgct gatggaatgg ctttttcgaa
    10501 gaaagataat gaaatgagtg aagcgacacg tgaagctctc attatttctt tagagaatgc
    10561 tttgcgaata gctaaaatag aagctaagaa aaaatacaca cctaaaaaat atcgcgatta
    10621 aaaggagtac gttgtttatg tatttacctc aaattgataa caaaattaat aagctggtaa
    10681 aactatatca aacacgcaat ccttttagaa ttgcacgaga attgaagata ctagttttag
    10741 aagaagattt gggagaaatt tatggttact atagtaaatt caaacagata aaaatgatcc
    10801 acataaattc tagtttagat gagatgggaa aatggataac ttgtagtcat gaattaggcc
    10861 actgtgtttt gcatccgaat gaaaacacac ctatgttgtc taaaaaaacg ctagtatctg
    10921 aattaaaaat tgaaaaagaa gcaaattatt tcgcaactca tctgactatt gatccaactt
    10981 tagacggctt tgatcatatg acaaaatatg aaaagctcgt ctgctttgga ttaccagatg
    11041 aattcgatcg tttcttgtga attataatac aacgtaagga ggatatagat gaaaatagga
    11101 atgagaaaac caagtataaa aaaatccata agcgctagaa ccactggaaa agcaaaaaga
    11161 aaagtaaaaa aagcagttat tcctggttat gggaaaaaag gtagcggctg gattaaagac
    11221 ccaaaaaagg ctgcttacaa taaagtatac aagaaaacta cattcagttt ttgggattta
    11281 tttaaataaa aaaagcccgt gcagcaacac ggactcgaaa ccttatttct aagattcccc
    11341 aataaaaatc atatcataga aatgaggaat aaaaaatgaa aaaagtaata acaagtttat
    11401 tagtttttgc ttctctgtta cttttcactg cttgtggtaa aaaagtaact acagaagatt
    11461 taaaagctaa tgattggttg attgaagcaa ccaaagatga cgaacctaat atgatcgtat
    11521 cattttctga tcacgtcgta tcttttagtg ttgacacaag ttctatgaaa tcaacttcaa
    11581 gtaacgattg ggaggctatg ggagaagaat ttgctaaaag cttagtcgac caaatgaatt
    11641 acaaatttga gtatacactt gaaggcgatg ttatgacttg gaaagatgat aaagacaaag
    11701 ataatgatgc caaatatact gtcagcaaag aagataaaaa tattatttta actccagaaa
    11761 agcctaacaa atctgatgat aatgagaaat tagttttgaa accctacaaa aaggaaaaaa
    11821 actaaagaat ctagaacaac tactagctca acaagtagca acgaacaatc atcttcgact
    11881 acaacagaaa gtactattgc atcttctagt actgtagaac aagctgctat tgcgcagaat
    11941 acgcctcctg aaactagtag tacttactct gctgaaagtg tagaaaacta tgcagaagct
    12001 actcctagtt caagtgttga acaagtttca cccactccca gttcaacgga gcaatcagta
    12061 ccacaagatt atgtaccagc taatcaagct caaccaacgg caccttcttc atctgaaacc
    12121 gaaaccttga ctttgcaacc tggtgaaggg accaaacaaa tagctgaacg agctggaatt
    12181 acggtagatc aactgtttgc attaaacggt atggatccaa acaactacat gctttatcca
    12241 ggacaagaac taagagtgaa ataaaaaaag cacatttgcc gcccgaccaa gaacgcaaat
    12301 gtgcaaagag ctacggtaaa gtaggtctat ttgttgtacc ctattttacc atctcgaaat
    12361 ccgctgtaca accgaacaaa cgtacgaaag gaaagataaa gatggcaaaa aaagaaattg
    12421 ataaacgaat taaaaagcac ttaacaaaaa aaggcgaaga aaaatatcag tttagtctgt
    12481 acttaggagt tgatcctcta acaggaaaaa aaagaaaaac aacgagacga ggttttagta
    12541 cgccattagc cgctgagaga gctttaaaaa gattggaggc agatatacag gaaaaaggac
    12601 tgcaagcttc tacagctcaa aagagcaaga aattcgaata tgtttacggc ttatggtttg
    12661 aaaattataa aaagacagtt aaagaaagca cttggtcctc caccaaacaa atttttgaca
    12721 cacacatctt aaaagtgttt ggagataagt tcattgacaa aatagacgtt tttttctgtc
    12781 aagaagcagt aaacacttgg tccgatagcc atcctaaaat ttttaagaaa ataaaaaatt
    12841 acacatctaa tgtttttgat tacgctgctt ctttacaaat tataactagc aatcctatga
    12901 aattagtttc tattccacgt ggagaa
//
LOCUS       DAYYAJ010000089        12826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000089 DAYYAJ010000000
VERSION     DAYYAJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.089"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..4494
                     /locus_tag="ACHATZ_002063"
     CDS             142..4494
                     /locus_tag="ACHATZ_002063"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8404581.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            4638..5111
                     /gene="rimP"
                     /locus_tag="ACHATZ_002064"
     CDS             4638..5111
                     /gene="rimP"
                     /locus_tag="ACHATZ_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8404582.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            5135..6310
                     /gene="nusA"
                     /locus_tag="ACHATZ_002065"
     CDS             5135..6310
                     /gene="nusA"
                     /locus_tag="ACHATZ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8404583.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            6332..6625
                     /locus_tag="ACHATZ_002066"
     CDS             6332..6625
                     /locus_tag="ACHATZ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8404584.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            6622..6933
                     /locus_tag="ACHATZ_002067"
     CDS             6622..6933
                     /locus_tag="ACHATZ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8404585.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            6946..9252
                     /gene="infB"
                     /locus_tag="ACHATZ_002068"
     CDS             6946..9252
                     /gene="infB"
                     /locus_tag="ACHATZ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8404586.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            9279..9626
                     /gene="rbfA"
                     /locus_tag="ACHATZ_002069"
     CDS             9279..9626
                     /gene="rbfA"
                     /locus_tag="ACHATZ_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8404587.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(9734..10238)
                     /locus_tag="ACHATZ_002070"
                     /pseudo
     CDS             complement(9734..10238)
                     /locus_tag="ACHATZ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10403..12766
                     /locus_tag="ACHATZ_002071"
     CDS             10403..12766
                     /locus_tag="ACHATZ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8404588.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         4451 a         2331 c         2808 g         3236 t
ORIGIN      
        1 aaattggaac tgcgatttga atcgcagttc caatttttta atgtaaaact cagaaaaaac
       61 gagtatacta aaaggtactg aaaaagaaga ggataagatg cgatcaattg cattgtttct
      121 catcagaaag ggcgtatatc tttgtcagaa aaacgagatt tatttgaaaa actgttggaa
      181 caaatccaac tagaggaagc agaaaaaaat catccactgc ttactgctgg tgaaatggat
      241 agtgtagtgg tccatcgtaa atcgcgttta tgggaattta cgctacattt ttccaaaatt
      301 ttgcctatta tgctatatcg gtcattgact cagcatctga ctatcgcttt taaagatatt
      361 gcacaagtca aactgaatat cttagcagat gaccagacat tcgatgaaca gctcttgcaa
      421 gattactggg cccaagcttt ggaaaatcag caatgcgata caccactagc acagcaagta
      481 ttgaaaacgc aggtacctgt gataaaagat cgtaaagtca tcttgccaat cgacagtaca
      541 ggagctattt cttatttgaa gcaacaatac ttgccattag tagaagagtt gttcgtcagt
      601 tacggattcc caaaatttcg tatcgaacca gaagtggatg agcagcaagc ggaacgtgta
      661 ttgaaacttt ttgaagaacg caaacaagaa caggcagaag cctttatgaa gcaagcagct
      721 gaaagtctag ttgtccacga acaaaagaaa aaggaaagaa aagagcaatc gccagctctt
      781 gaaggaccca tccagctagg acgaaatatt ccagccgatg aaccaacgac accaatgatc
      841 agtatcgttg aagaagaacg aagagtcaca cttgaggggt acgtattcga caaagaagtc
      901 agagaactac gttcaaaacg caagatcttg actttgaaaa tcacagatta tacttcatca
      961 tttatcgtca aaaaattctc gaacggcgaa aaagatgaac aagtatttga tgcgatcagt
     1021 gtcggtagct ggttaaaggt acgaggaagt attcaagaag atacgtttgt ccgtgatttg
     1081 gtcatgaatg cgcaagatat catagaagtc aaacatacac ctcgcaaaga ttatgctcct
     1141 gaaggggaaa aacgagtaga gcttcatgtt catagcaata tgagtacgat ggatgcaaca
     1201 aacagcatct ctgatcttgt ggcacaagca ggaaaatggg gacaccgagc tatcgccatt
     1261 acagaccatg gtggtgcgca agcctttccg gaagcacata gtgctggaaa aaaagcaggt
     1321 gtgaaaatct tatacggcgt ggaagcgaat gtcgtagatg atggtgtccc aattgcttat
     1381 aatgatgcac atgaagcatt gagtgaagcc acatacgtcg tatttgacgt ggaaacgaca
     1441 ggattgtctg cagtatatga cacgatcatc gagctagccg cagtcaaaat gtataaagga
     1501 aatgtgatcg aaagctttga tgaatttatc gatccaggac atccattgtc acgaacaacg
     1561 gtagatctga ctggtatcac ggatgaaatg gttcgagggt caaagtcgga agaagaagta
     1621 ttgcggatgt tccttgaatt ttcaaaagat acaattttgg tagcccacaa cgcggctttt
     1681 gatatggggt tcttgaatac tagctacgca aggtatggta ttcctgaagc ggccaatcca
     1741 gtcattgata cgttggaatt agctagatat ctgtatcctc aattcaaacg tttcggatta
     1801 ggtgtgttat ctaaaaagtt tggtgttagc ttggaacagc atcaccgagc aatctatgat
     1861 gctgaagcaa ctggtcactt agcatggatc tttgtgaaag aggcaatgga caatcataac
     1921 atgctttatc atgatcagct aaatgagcat atcggagaag gagattctta caaaagggct
     1981 cgacctttcc atgtaacgat tttagcaaaa aatcaggcag gactgaaaga tttgttcaaa
     2041 ctgatttcaa tgtcgaatgt cgaatacttc gaacgcgtcc cacgaatacc acgctcccaa
     2101 ttgaaaaaga tgcgtgaaaa tctattgatc ggctcagctt gtgataaggg agaaatattc
     2161 gaagccatga tgcaaaaagg ggtagaagag gcaagaaacc gagcaaaatt ctatgattat
     2221 attgaagtta tgccaaaagc ggtctatgcg cctttgatcg agcaagaatt ggtaaaaaat
     2281 gaacatgatt tggaagaaat tatccaaaat ttggtagaaa tcggaaaaag tttggataaa
     2341 atcgttgttg caacaggtaa tgtacattat ctgaacgaag aagatgccat ttaccgaaaa
     2401 attttgatca attctatggg cggagcgaat ccgttgaatc gtcatagttt gccggatgtc
     2461 catttccgaa caaccgatga gatgctaact gcatttcact ttttagggga agaaacagcg
     2521 aaagagatcg tagtagaaaa tacgaataaa atcgcagata tctgcgaaga agtgattcca
     2581 gtcaaagatg aactttatac accgaagatt ccaggttcag aagatgaaat atcagaattg
     2641 agttacacaa aagcaaaaca aatgtacgga gatccgttac ctgagatcat tcaaaaacga
     2701 ttagaaaaag agttgaattc tattaatggt aatggcttct cggttattta tctgatcgcc
     2761 caaaaactag tgcataaaag taatgaagat ggctatctag taggttcacg tggctctgtt
     2821 ggctctagct ttgttgcgac gatgacagga atcactgaag ttaatccatt agcccctcat
     2881 tattattgtc cagagtgcca atattccgag ttttttgaag atggtacgta tggttctgga
     2941 tttgacatgc cagaaaaaca atgtccaaaa tgtggtgcac gcttaaacaa agacggtcat
     3001 gatattccat ttgagacatt tttaggattc catggagaca aagtgcccga tattgatttg
     3061 aacttctcag gtgattatca agcagaagcc cataactata cgaaagtatt gtttggcgaa
     3121 gactatgttt accgtgccgg aacgattggt acagtcgccg acaaaacagc atatggctat
     3181 gtaaaaggat acgaaaggga caataatctg cagtttcgaa gtgcagaagt cgacagatta
     3241 gcaaaaggcg ctactggagt caaacggaca accggccagc atccaggggg gatcatcgtt
     3301 attcccgatt atatggatgt ctatgatttt acgccgatcc aatatccagc agacgatcaa
     3361 aattcagaat ggaaaacgac tcattttgat ttccactcga ttcacgataa tgtattgaaa
     3421 ttagatattc tagggcatga tgacccaact gttattcgga tgcttcaaga tttatcaggg
     3481 atcgacccac aaacgatccc tacggatgac ccggaagtga tgcggatatt tgccggtcct
     3541 gaagtacttg gagtaagtca ggaacaaatt tattccaaaa cagggacatt aggtattcca
     3601 gaattcggca ctcgttttgt tcgagggatg ttagaagaaa cgcatcctac aacttttgca
     3661 gaactactgc aaatttccgg actatctcat ggtacagacg tatggttagg aaatgcggaa
     3721 gaattgattc gacgaggaga tgcgacactg gcggaagtaa tcggctgtcg tgatgatatt
     3781 atggtttatt tgatccatgc tggattagat agcgggatgg catttaaaat catggaaact
     3841 gttcgtaaag gacaatggaa caagatccct gatgaattgc gagaaaccta tctatcggcg
     3901 atgaaagaaa ataatgtgcc cgactggtat atcgattctt gttcaaaaat caaatatatg
     3961 tttccgaaag cccatgctgc agcttacgta ttgatggctc ttcgtgtcgc ttatttcaag
     4021 gtttatttcc ctattttgta ttactgtgca tacttttctg tacgtgcaga cgactttgat
     4081 ctagtctcca tgtgcaaagg aaaagatgct gtgaaacaag caatgaaaga aatcacggac
     4141 aagggattag atgcttccgt aaaagaaaag aatcaactga cagtgctgga gttagccaat
     4201 gaaatgctgg aacgaggatt caaatttgga atgattgatt tgtataaatc ggatgccgtt
     4261 aactttgtta ttgaaggtga tacgttgatc gcaccattta gagctgtccc aagtttggga
     4321 accaacgtgg cgaaacaaat cgttgaagca agaaaagacg gtccattttt atccaaagaa
     4381 gatcttgcca cccgcggtaa agtttcaaaa acattgatcg aatatatgaa cgacaatggc
     4441 gtattgaaag atctaccaga tgaaaatcaa ttatcgttat tcgatatgct ataagcatct
     4501 gttttcctgt ctgcttgtaa ttttaagcag agtatggtat agttcttata ggtaatgata
     4561 ctaactgtga gtgagcggat ttttcgctca ctctttttaa tggaattacg cacttaattt
     4621 ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt cacagaaatg gtgacaccga
     4681 ttttagatga acaaaatttt gaacttgtcg aagtcgaatt tgtaaaagaa ggaaaaagct
     4741 ggttcttacg agtatttatt gacaaagaag gcggtatcga tatcgaagaa tgcgcctttg
     4801 tcagtgagaa actgagtgaa aaacttgatg cagcagatcc agatccgatt cctcaagctt
     4861 atttcctgga ggtatcctcc ccgggagcag agcggccatt gaaaaaagaa agcgactatg
     4921 aaaaggcact tggtgattat gtccatgtct ccttatacca accagtagat ggcgaaaaac
     4981 aatatgaagg atttcttcaa tcatttgatg ctgaacagct tacattgaaa atacgcataa
     5041 aaacacgaga aaaagaattg acttttgacc gaaaaaatat tgcaaaagct cgcttagcta
     5101 tccaatttta atcacggaga ggaatcagaa aaaaatgagt aaagaaatgt taaatgcgtt
     5161 agacgcttta gaagctgaaa aagggatctc aaaagaaatc gtgatcgatg ctttagaagc
     5221 agcattggtt tctgcataca aacgtcacta tggccaagca caaaacgtcg aagtggaatt
     5281 tgatcagaaa aaaggaaaga tccatgtcta tgcggtaaaa gaagtaacgg aagaagtgat
     5341 ggattcacag ttggaagtct cattgaaaga tgcactttta atcaatccag catatgagat
     5401 cggtgatacg atccgttttg aagtaacacc aaaagatttt ggacggatcg ctgcgcaaac
     5461 ggctaaacaa gttattttac agcgtgtacg tgaagcagaa agaacgatta tttataacga
     5521 attcagtgct tatgaaaaag acatcatgca aggaatcgtc gaacgccagg ataaacgtta
     5581 tatttatgtc aatcttggta aaattgaggc agttctttct aaacaagatc aaatgccaaa
     5641 tgaattttat caaccacatg atcgaatcaa agtttatgtt tcccgagtag aaaatacgtc
     5701 taaaggtccg caagtatttg ttagccgcag ccatccagac ctattgcgtc gcttatttga
     5761 acaagaagta ccagaagtct atgatggaat cgttgaaatt gtcagcatcg cccgtgaagc
     5821 tggcgatcgt tcaaaagtgg cagttcgctc aaatgatcca aatatcgatg cagtcggtac
     5881 atgtgtcggg ccaaagggac aacgagttca agcaatcgtc aatgaattaa aaggcgaaaa
     5941 catggatatc gtcgaatgga atgaagatcc agctgtattc atcgcaaatg cattgaatcc
     6001 tgcacaagtc atggatgtga tcttcgatga gacaaatcca aaggcctgca cagttgtcgt
     6061 tccagactac caattatcgc tagcaatcgg taaacgcgga caaaatgcac gtttagctgc
     6121 taaattgact aaccataaaa tcgatatcaa atcagaatct gacatgacgg aattttatga
     6181 aaatcaagaa caacaaaaag aaacagaaga actgcatgat gaagcaatcg ttcagtcaga
     6241 tttaacagac gatgaatatg aaacaattgc attcaacaat gaaacggtcg aagaaaaacc
     6301 agaagcttaa ctttagtaag gaggcaaaga gatgaaacaa aggaaaattc ctttacgcaa
     6361 atccgtggtt tcaggtgaaa tgaaacctaa aaaagagatg gtccgaatca cccgttcaaa
     6421 agaaggggaa gtatcgatcg atccaactgg aaaaatgcct ggacgaggag cgtatgtctc
     6481 acttgagccg gaagaagtac aacaagcttg ggataaaaaa attctcgatc gagtactcga
     6541 aacaacatta acagatgagt tttatcaaga acttcttgac tatgtcactc accaaaaagc
     6601 acgtaaagag ttatttggaa aatgaataca gttaatcggc aaaaagccat gaatctcatc
     6661 ggtctagcga tgcgcgcggg aaaactaatc acgggtgaag aactgaccat tgctgaaatc
     6721 cgcaaacaaa aagcaaaatt ggtgtttgtg gcaaccgatg caagtgagaa tacaagaaaa
     6781 aagataaaag ataagagttc atattatgaa gttccttgct ttgagctatt ttctgaagca
     6841 gaaatcacgc agatgatcgg aaaacctcgc aaggtattcg gaataatgga tgccggattt
     6901 gcgaagaaga ccaaggagct aattgaaggt taggaaggtg attgcatggg caacaaaaga
     6961 atctatgaat tagcaaaaga actcaaaaaa tctagtaaag acgtcgtgga aaaagcacaa
     7021 caacttggca tcgatgtgaa aaaccatatg ggtgcgatta ctagcagtga tgagaagaaa
     7081 ttgcaacaag cgttcaaaaa cccaagcacg aaacaacaag ctgcacaaca agcaagcaga
     7141 aaaccagcca atcaacagcc gaacgatcaa cgtaaaaaag aaacaacgaa caatcaacaa
     7201 aaaaataaaa ataaccgcag ttatcaagat cgcggtcaag ggagcggtca agtgaatcaa
     7261 ggaaaaaaac caacaacaaa ccaaaataat acgggaaata ccagcagcca aaatcgtcaa
     7321 ggaacgagcc aaggcggcaa tcaaaaccgt actaacaata acaacaaacc aaacaataac
     7381 aatcgcggca aatttaataa taataaccgt aatcgttata ataaaaaagg taaaaaaggc
     7441 aaacagcaac aatcaactaa accagctgta ccaccacgca aattccgtga attgccagat
     7501 gtattagagt atactgaagg tatgaacgta gcagatattg cgaaaaaaat tcatcgtgag
     7561 ccagcagaaa tcattaaaaa attatttatg cttggtgtca tggtcaatca aaaccaagca
     7621 ctggataaag atacgattga actgttagcg acggactatg gcatggaacc acaagaaaaa
     7681 gtccaagttg acattgctga tatcgataaa ttcttcgagg cagaagaagt cgatccagat
     7741 aaattagtct cacgtccgcc agtagtaacg attatgggac acgtagacca cgggaaaaca
     7801 accttattgg atacattgcg ccattcacgt gtaacaagcg gcgaagccgg cggaatcaca
     7861 cagcatatcg gtgcctacca gatcgatatt gatggtaaac caatcacttt cttggataca
     7921 ccaggacatg cggcctttac aagtatgcgt gcacgtggag caagtatcac agatatcacg
     7981 atcttagttg ttgctgcaga tgatggcgtc atgccacaaa cagttgaagc aatcaaccat
     8041 gccaaagctg caggcgttcc aatcatcgtc gctgtaaata aaattgataa accaggtgca
     8101 aatcctcagc atgttatgca agaattaagt gagtatgaat taattcctga agcatggggt
     8161 ggagaaacga tttttgttga gatctcagcg aaattcggcc aaaacatcga agaattgctg
     8221 gaaatgatcc tgttagtcgc agaagtagaa gatttaaaag cagatccaac acaacgcgca
     8281 atcggaacag tgatcgaagc acgtcttgat aaaggtaaag gtcctgtgtc aaccttactt
     8341 gtccaagaag gaacacttca cgttggtgac ccaatcgttg tcggtaatac ttacggacgt
     8401 gtccgtgtaa tgacaaacga tcttggccgt cgcgataaag aagcaggacc tgcaacgcca
     8461 gtagaaatca ctggcttgaa tgatgtacct caagcaggtg accgtttcgt agtcttcgaa
     8521 gacgaaaaga cagcacgtgc agcgggtgaa gaacgcgcaa aacgtgcatt attggaaaac
     8581 cgtgctgcca caagccgtgt gactttggac aacttatttg aaagcctgaa agaaggcgaa
     8641 ttgaaagaag ttaacgtaat catcaaagca gatgtacaag gttcagcaga agcactggct
     8701 gcatcattga agaagattga tgtggaaggt gtccgtgtaa aaatcgtcca ttcagctgtc
     8761 ggagcaatca atgaaagtga tgtaacctta gcagcagcca gcaacgctat tatcatcggt
     8821 ttcaacgttc gtcctacacc gcaggcaaaa gtgcaagctg atactgaaga agtcgatatt
     8881 cgtcttcacc gtatcatcta taaagccatc gaagaaatcg aaactgcgat gaaagggatg
     8941 ctagaccctg aatttgaaga aaaaatcacc ggtcaaatga tcgttcgtga aaccttcaaa
     9001 gtttctaaag ttggtacgat tgcaggtgcc tatgtaacag aaggatatat tcgtagagat
     9061 agcggtgtcc gtgtaatccg tgacggaatc gtaatctatg aaggccaact tgcaagtctg
     9121 aaacgattca aagatgacgt gaaagaagtc aaaatgggtt atgaatgtgg tgcgatgatt
     9181 gaaaaattca atgacatcaa agtagatgac gtaattgaag gatttatcat ggaagaagtc
     9241 aaaaatgact aaaaactgat aaaaggaggc caatcattat ggctaactac cgtgatcgcc
     9301 gagtcggtca agaaatctta aaagaagtca atgatatttt gcgcaaaaag gtccgtgacc
     9361 ctcgtgtgga gaacgtaacg atcacagatg tgaaagttac gggggactta cagcaagcaa
     9421 cgatctatta cagcatttta tctgacttag catcagataa gaaaaaagca caagatggat
     9481 tgaataaagc atcaggattg attcgtcgag agttagggca caatctaagt atttataaga
     9541 cacctgaatt gttctttgaa cttgatgaat cagttgtcta tgggaaccat atcgatgaat
     9601 tgcttcgcga cttgaacaaa gactaatcaa aaaatcccgc aaatcctgtg aaatcagggg
     9661 ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg actttagtct
     9721 ggtagcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg actttctttt
     9781 ttctatagta tataaatttt tgcatataga taaagatcaa gataccgata acggaagcta
     9841 gattgtaatc tattttagtg acagtcaggt atagccaagt aactagaaga tcagtggaac
     9901 ctgcaccata aaagtttgag tttgtcggtc cgttgacagt taaaagaaaa atcacgttga
     9961 agttattgat atttccgatt acgccgcttc gatttggtca cgatccaaat tttggataat
    10021 ccctgtagaa attagcatag tagctgggat accaattcac atattgacaa agataacaga
    10081 gacttttaca taaaacggat caatcaggaa aggaattgct cgatccggcc ccagttttcc
    10141 aacaatgtgt taactggcct agcgtcattc agcaaattat gcatgatgag gagcgaaatg
    10201 aactgcggaa cagccattgt gataacgaca agttttttgg atcaatgtaa aaaacgtcag
    10261 cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt ggctagatat aaaatccaaa
    10321 caaatgaaca gaagtgctta ttaggtttca attgggagta tctgcgctag aataagagta
    10381 ataatgaaaa ggagtgttat gtatggatta ttctagtaaa gaatactttg ataaaatgac
    10441 ggcttggtgg cgtgctgcta attatctttc tgttgggcaa ctttatctta aagataaccc
    10501 cttactgaga cgtacactga aaccagaaga tgtcaaaaaa catccaatcg gtcattgggg
    10561 aacgattcct ggccaaaatt ttatttatgt ccatttgaac cgagtcatca ataaatatga
    10621 tttgaacatg ttttatatcg aagggccagg acacggcgga caagtcatgg tatcgaatgc
    10681 ttatttagat ggaagttaca cagagatcta tccagaagta acggaagatg agacaggaat
    10741 gcaaaaatta ttcaagcgat tttcatttcc tggaggcatt gcttctcatg cagcaccaga
    10801 aacaccaggt tctatccatg aaggcggaga gcttggatac tctctttcac atgcagtcgg
    10861 agctgtatta gacaatcctg aagtaatctc tgctgtcgtg attggtgatg gtgaagcaga
    10921 aaccggtcct ttagctggaa gctggttctc aaatgtcttt atcaatccag tgatagatgg
    10981 agctgtttta ccgatccttc atttaaatgg tgcaaaaata gccaacccaa cgatcttggc
    11041 acgtaaatca gatggagaat tagcaaatta ctttaacgga cttggctggg agcctttctt
    11101 tattgaagga aatgatccag aaaaattaaa tcctgttatg gctgaaaaaa tggatcaagc
    11161 aatcgagaaa atcaaatcca tccaaaaaga agctcgcctg aagacagcaa cagatgtcgt
    11221 gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa ggatggaccg gtccaaaaga
    11281 atgggacggg gaaccaattg aaggaacctt ccgagcacac caagtaccga ttcctgtaga
    11341 tcaagaacat atggatcatg cagatgcttt actccgttgg ctgaaatcct atgaaccaga
    11401 aaaattattt gatgcacaag gacgtatttt agaagaaatc agagaaattg cgcctactgg
    11461 tgaccaaaga atggcaaaaa atcctatcac caatggtgga atcgatccaa gaccgcttat
    11521 tatgccagat tggaaaaaat atactttgca atttgaaaaa ccaggctccg ttaaagcaga
    11581 agatatgacc gaacttggta aattcgttcg cgagatcatt gagaaaaatc cagaaaattt
    11641 ccgtatattt ggaccagatg aaacaaaatc aaaccgattg aatcaagttt tcaaaacgac
    11701 aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat gaatggcttt cgccatctgg
    11761 tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa gggttcttag aagggtacgt
    11821 tctgactgga cggcatggtt tttttgcaag ttacgaatca ttcttgcgag tggttgactc
    11881 tatgttgact cagcacttca aatggatacg gaaatcacat gacctttctt ggcgaaaaga
    11941 ttatccttcg ctgaatctca ttgcaagctc aacagtcttc cagcaagatc acaatggata
    12001 tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg agttcatccg
    12061 tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag cattccgctc
    12121 gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat tttactcagc
    12181 agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg cttcgacagc
    12241 aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta acttagaagc
    12301 attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc ggttcatcaa
    12361 tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt tgagcgatga
    12421 agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc acggttatga
    12481 aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga ttcatggcta
    12541 caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg aacttgatcg
    12601 ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag aagattttgc
    12661 ggaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa atggtattga
    12721 tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg ataaaatcga
    12781 tcccgcaaaa cagaattttt tacattgtat tatttgtcaa attaag
//
LOCUS       DAYYAJ010000090        12738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000090 DAYYAJ010000000
VERSION     DAYYAJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.090"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACHATZ_002072"
     CDS             97..540
                     /locus_tag="ACHATZ_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8404589.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACHATZ_002073"
     CDS             complement(546..1730)
                     /locus_tag="ACHATZ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8404590.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACHATZ_002074"
     CDS             1998..2696
                     /locus_tag="ACHATZ_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8404591.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACHATZ_002075"
                     /pseudo
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACHATZ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3506..4039)
                     /locus_tag="ACHATZ_002076"
     CDS             complement(3506..4039)
                     /locus_tag="ACHATZ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404592.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACHATZ_002077"
     CDS             complement(4336..6717)
                     /locus_tag="ACHATZ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8404593.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHATZ_002078"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHATZ_002078"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8404594.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACHATZ_002079"
     CDS             7418..7957
                     /locus_tag="ACHATZ_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8404595.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHATZ_002080"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHATZ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8404596.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACHATZ_002081"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHATZ_002081"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="ACHATZ_002082"
     CDS             9064..10230
                     /locus_tag="ACHATZ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8404597.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="ACHATZ_002083"
     CDS             10276..11772
                     /locus_tag="ACHATZ_002083"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8404598.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="ACHATZ_002084"
     CDS             11853..12536
                     /locus_tag="ACHATZ_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8404599.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12545..>12738
                     /locus_tag="ACHATZ_002085"
     CDS             12545..>12738
                     /locus_tag="ACHATZ_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404600.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4272 a         2230 c         2630 g         3606 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggttga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DAYYAJ010000091        12704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000091 DAYYAJ010000000
VERSION     DAYYAJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.091"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..473)
                     /locus_tag="ACHATZ_002086"
     CDS             complement(237..473)
                     /locus_tag="ACHATZ_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404601.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(656..1027)
                     /locus_tag="ACHATZ_002087"
     CDS             complement(656..1027)
                     /locus_tag="ACHATZ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8404602.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1024..2352)
                     /locus_tag="ACHATZ_002088"
     CDS             complement(1024..2352)
                     /locus_tag="ACHATZ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8404603.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2313..2642)
                     /gene="mobC"
                     /locus_tag="ACHATZ_002089"
     CDS             complement(2313..2642)
                     /gene="mobC"
                     /locus_tag="ACHATZ_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8404604.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2900..3270)
                     /locus_tag="ACHATZ_002090"
                     /pseudo
     CDS             complement(2900..3270)
                     /locus_tag="ACHATZ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3415..3600
                     /locus_tag="ACHATZ_002091"
     CDS             3415..3600
                     /locus_tag="ACHATZ_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8404605.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3758..3925
                     /locus_tag="ACHATZ_002092"
     CDS             3758..3925
                     /locus_tag="ACHATZ_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404606.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4543..5202
                     /gene="vanR-B"
                     /locus_tag="ACHATZ_002093"
     CDS             4543..5202
                     /gene="vanR-B"
                     /locus_tag="ACHATZ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8404607.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5205..6566
                     /gene="vanS-B"
                     /locus_tag="ACHATZ_002094"
     CDS             5205..6566
                     /gene="vanS-B"
                     /locus_tag="ACHATZ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8404608.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6657..7958)
                     /locus_tag="ACHATZ_002095"
     CDS             complement(6657..7958)
                     /locus_tag="ACHATZ_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8404609.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            8237..9043
                     /locus_tag="ACHATZ_002096"
     CDS             8237..9043
                     /locus_tag="ACHATZ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8404610.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9061..9768
                     /locus_tag="ACHATZ_002097"
     CDS             9061..9768
                     /locus_tag="ACHATZ_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8404611.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            9765..10736
                     /gene="vanH"
                     /locus_tag="ACHATZ_002098"
     CDS             9765..10736
                     /gene="vanH"
                     /locus_tag="ACHATZ_002098"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH"
                     /protein_id="HFM8404612.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSTDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10729..11757
                     /gene="vanB"
                     /locus_tag="ACHATZ_002099"
     CDS             10729..11757
                     /gene="vanB"
                     /locus_tag="ACHATZ_002099"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8404613.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            11763..12371
                     /locus_tag="ACHATZ_002100"
     CDS             11763..12371
                     /locus_tag="ACHATZ_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8404614.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(12437..12610)
                     /locus_tag="ACHATZ_002101"
     CDS             complement(12437..12610)
                     /locus_tag="ACHATZ_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404615.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3370 a         2802 c         3176 g         3356 t
ORIGIN      
        1 cctattccta cgcatttaga acccggtatt gtcggggata ggatgaagta ttcgcttgcc
       61 cctgcaaaca tgcgggagaa gccttgcagc gggcggtttt caatccctta aatgcgtagg
      121 ttgcagctat aattagaccc ctgaaacaga tatagccgcg cagcgggtgt atctgtttca
      181 ggggtgtgca ggtgatagcc gcaaagcggc gtaagagggt gcagccctct tgccgtctat
      241 ctctgttcca gcgggaagct cggcaatgct tccagcagtt tcagggaaat gcgctgccgt
      301 atatcttcgt caatgacttt cttctttttg ccgtttgcct gtctgatctc caccgtggaa
      361 agctcgttga tgtagtcagc ataatgggcc aacacttttt ccgtggccca cctctcaccg
      421 gcaacggcgg ctttgatcgt ttcatagtcc agcagctttt cttgtccgtc catcctgata
      481 cccccgtcag actgattttt tctatgatac catattcgga agcatggaac cagagaaaac
      541 ggcgcttttc tgctggtaaa ttctacgcat ttagaacagc acttcggcgg ggataggata
      601 aaacacccac ataccccgct gaccatgccg gaaaagcctt gaaatataag ggcttttaca
      661 gctctaagtg cgtaggtttc cagtatcttt tccgctggcg ctcggcctct ttgcggcggc
      721 gcatccgttt ggagcaggcc gcgcagtatt tggcccggtt agacttcggc gtgaacgtgg
      781 ccccgcactc acagcagcgt ttcacttcgt cccggtcttt ggtgatctcg gcatacaggg
      841 ccgcatccag cgggaggaca gcggccttaa accagcggca cagaagcgaa taggaaatgc
      901 tctgtacgca aatacattct tcgccgtcgt ccagcaacag gcagttgccg tcacaatagt
      961 tgcagcactc atgcgtcagc ttccgggcct tgcgatattg agggtaattc attcgtggaa
     1021 tactcatagc tcttgcgtcc attcctttcc cggcgtcata cgcaaaatgc cgtccacatt
     1081 ctgcttgatg atgccgtatt cctgcatctg ctttttcgct tcgccgtact tctcatacag
     1141 ccgttccttt tcctcggcca atcgcttgta ctcggctttc agggcgtaca gatcggggag
     1201 ctttttcaca ccctgtccct ttagggcctt tgccgcagct tcatacaaaa tcaacgtgct
     1261 ttcatgctca cgccggaatt ttgccttatc cgctgcattg cggtattcca tagccacggg
     1321 ccagagctgc ttgtaggtag aaatattctt tatcagcacc gccatatccg caagccggtg
     1381 ttccatagct ttgagggtag cggcggcctc gtcgctggcg gcggtgatct cggccacttt
     1441 gctttccaaa tccgggtagg tgtctatccc atgttcagtc aggaaattca aagtctttgc
     1501 ggcctgcttc aaattgtgga gcttcgccca cctctcatag cctgccgact gctgggcctt
     1561 aatgttgttt tccagatcaa tccgcaaaga aatccccttg cgctctttgg gcgctctggt
     1621 gataacgcgg cctttgatac gctctttgat ggcttcctcg gtgtaggctt cccccaatgt
     1681 cttgcagcgg gtaaagcgtt cctgtccggg agcgcggaaa gaaataaact ttcccggctt
     1741 gatctcatag ccctgcgcct gcatgagccg caggaaatca tcaaaatctt ttgcctgcgg
     1801 gatagcggcg tcaatggctg ctttgagctt cgctttccag ctcgtccctt tccgctgggc
     1861 gtcccactcg gcatagctct tgcccttact ctggccgggc ttcaccacgg acaatccgtt
     1921 ttccttgcac agcctgtcgc tggtgcgccg gatataggca tagctctgtt tgttggaaat
     1981 gtacttgcgc tgatttacca tatccacggc acagaaaata atgtgattgt ggacgtggcc
     2041 cttatcaata tgggtagtca gcacataggg atatttccct tgcagcactt cctcggcaag
     2101 ctgcttcccg atctcatggg cctgttccgg cgtggcttcg ccgggggcaa aggattgtat
     2161 caggtggtgg cccagattat tgcccttttg gtatgcctgc gccagcagca tttcaaattc
     2221 aatgtccgct gtttcataag agcaggcaaa ggaggccacc agcagcttgc cgtcagtttt
     2281 gtccgggttc tcgatgtagt caagggcttt tttcagcgtt gacttgatag gatgtatctt
     2341 tgtaactgcc atatctgcgc cagcgccccc tttatatcct cgatgtcctg tgcgtacacc
     2401 ggccccatac cgttcagcct ctttgcaatc tgattgacgt tgacgccgat cttctgtatc
     2461 tcggcggtct gcgcccggat gtcgctggtg tccatgttga cgatatagcc gtcaatgagc
     2521 atcttccggg cgtaggctga aaagttcttc gtatgcagca gcgccatttt ctgttcaatc
     2581 atagcccgtt cctccggcgt cacagggacg cgcagcacga tttttcgctt tctgtttgcc
     2641 atgcttcacc gtcctttcca ttcaggggtt tggggatttc cccaacaagc aaaacggacg
     2701 cggcccggca gggacggggc aggccgtttt tccgggagtg gctacactcc ctatgcttgc
     2761 tacgttactg tttctccact aacaagacgt ttccaaagcc ccaaactacc cgcaaaatag
     2821 ccctctggaa agaaaaaagt gcaaaaaaat agaagctacg ctgtgcagct cccgctaaaa
     2881 gtaaaacaga ggatgccagt tacacatcct ccgtttcatt cttcttcggc ttttcaatta
     2941 gtatggcgct gtcaatcgcg ccctcgatga tcggcaggta ttcttcgggg cagagtttca
     3001 gcttgtggct gaccctttgc cgcagttcgc tttcctcccg catgatctcc gggttaaagt
     3061 agcgttccac aggaagcccg caggctttaa taagctgtat gacaaccggc agactgggaa
     3121 tggcccgtcg ttttcgatgt tggcaagata gcgccagtca atatttattt tctctgccat
     3181 tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc acatccgcgc cgaaagtttc
     3241 aaagccggga caatcttcaa tattcgccat atcgcacctc ccacttacat tgtatatttc
     3301 atactctgcc cgtggaatgt tggtatatgc acctttctta aagtttatat ttcacatttt
     3361 gtttctttta ttcttcgagc aaaagaatta taatgtaccc tgtggaggtg tactatggac
     3421 tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc ccccacgatg gataaactac
     3481 tattgtgctg cgggacgcat ccccggcgct gtaaagatgg cgacgatctg gctcattccc
     3541 aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag gaaaggcggt tactgaatag
     3601 tataatgaaa ccggcgcatt ttggatatgg ttttgaagag agagaaagca tggtattttg
     3661 tcacggaaaa actataagat gatagatggc aggctgctcc agaccgataa aaagtattct
     3721 catctgaaac agaaagaaaa gatttacaca tggatgtatg aagaagcgaa actttatcat
     3781 gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct gctgctgtat cagccgtata
     3841 tggtagaatt gaaaaggccg gcatctggat tctttatggt gaagtctcca gacattggat
     3901 aactgctggt ctaatgcgcc tttgagtatg gggtgattga ttttaatacc ggggaggtac
     3961 ggctttttga ggaaaatatt ctgggattta catttggcga ttattacatt tcctgtaact
     4021 gcaaggacac ggatgatgaa gatatgagga atatagactt gctgattttc tactgtcttg
     4081 aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa gagcgaccgg agggagtaca
     4141 cacaggccct gcctgtgtga aaagccatac cgcaaggtgc attcgcaaaa aagaggccgc
     4201 gcattttgct gaggcttggg gtgtccccaa caagcaaaac gagaacgccg caggcgggca
     4261 agtggtggat cagaatatcc gtaaaggata ttctgatcca ttgcttgcca gtattatcgg
     4321 gatgttttga caaccgctgt aatcgaaaca tcccaaacat taagaaggtg ttaattatta
     4381 ttatcttgat ttaattcgtt taaaccaata atatagatac atacaccatg aaaagaagcg
     4441 agtatcccaa tgacggatga ttaaaatccg aaaagaaatc gctttgaaac tacagggaaa
     4501 ctacagactg ttatgttatc ttcttaaaat ggagggattt ttatgtcgat acgaattcta
     4561 cttgtcgagg atgatgatca tatctgtaat acagtaaggg cgtttctggc tgaggcagga
     4621 tatcaggtgg atgcctgcac agatggaaat gaggcataca ccaagtttta cgaaaacact
     4681 tatcaactgg ttattcttga tattatgctg cccggtatga acgggcatga acttttgcgt
     4741 gaatttcgtg cgaaaaatga tactcccatt ctgatgatga cagccctgtc ggatgacgaa
     4801 aaccaaatcc gggcgtttga tgcagaggca gacgactatg taacaaagcc attcaagatg
     4861 cagattttac taaagcgggt ggaagccctg ctgcggcgca gcggtgcgct ggcaaaggaa
     4921 atccgtgtcg gcaggctgac acttctgccg gaggatttca cggtactttg tgacggtaca
     4981 gagctgcccc tgacacgaaa agaatttgaa atccttttgc tgctggtgca gaacaaaggc
     5041 agaaccttaa cgcatgaaat cattttgtca cgtatatggg gatatgactt tgagggtgat
     5101 ggcagcacag tccacactca tatcaaaaat ttgcgggcca agctgccgga aaatatcatc
     5161 aaaaccattc gcggtgtagg ataccgattg gaggaatcat aataatggaa agaaaaggga
     5221 ttttcattaa ggttttttcc tatacgatca ttgtcctgct actgcttgtc ggtgtaacag
     5281 caacactgtt tgcacagcaa tttgtgtctt atttcagagt gatggaatta cagcaaacag
     5341 taaaatccta tcagccattg gtggaactga ttcagaatag cgataggctt gatattcaag
     5401 aggtggcagg gctgtttcac tacaataacc aatcctttga gttttatatt gaagataaag
     5461 agggaagcgt actctatgcc acaccaaatg ccaatacatc aaatagtttt agacccaact
     5521 ttctttatgt ggtacataga gatgataata tttcgattgt tgctcaaagc aaggcaggtg
     5581 tgggattgct ttatcaaggg ctgacaattc ggggaattgt tatgattgcg ataatggttg
     5641 tattcagcct tttatgcgcg tatatctttg cgcggcaaat gacaacgccg atcaaagcct
     5701 tagcggacag tgcgaataaa atggcaaacc tgaaagatgt accgccgccg ctggagcgaa
     5761 aggatgagct tggcgcactg gctcatgata tgcattccat gtatgtcagg ctgaaagaaa
     5821 ccatcgcaag gctggaggat gaaatcgcaa gggaacatga gttggaggaa acacagcgat
     5881 atttctttgc ggcagcttct catgagctaa aaacgcccat cgcggctaca agcgttctgt
     5941 tggagggaat gcttgaaaat atcggtgact acaaagatca ttctaagtat ctgcgcgaat
     6001 gcatcaaaat gatggatagg cagggcaaaa tcatttccga aatactggag cttgtcagcc
     6061 tgaatgatgg gagaatcgta cccatagctg aaccgttgga catagggcgc acggttgccg
     6121 agttgctgcc cgattttcaa accttggcag aggcaaacaa ccagcggttc gtcacagata
     6181 ttccagccgg gcaaattgtc ctgtccgatc cgaggctgct ccaaaaggca ctatccaatg
     6241 tcatattgaa tgcagttcag aacacgccgc agggaggcga ggtacggata tggagtgagc
     6301 ctggtgctga aaaatgccgc ctttttgttt tgaacatggg cgttcacatt gatgatactg
     6361 cgcttccaag gctgttcacc ccattctatc gcattgatca ggcgcgaagc agaaaaagtg
     6421 ggcgaagcag aaaaagtggg cgaagcggtt taggacttgc catcgtacaa aaaacgctgg
     6481 atgccatgag cctccagtat gcgctggaaa acacctcgga tggcgttttg ttctggctgg
     6541 atttaccgct cacatcaaca ttgtaaatat ttaaaactta aatgattttg ggctctttgt
     6601 caaataggac tgatagagtg agataagaac atctaaggta gaattccttt tctactttag
     6661 atgttttttg attcataggt aatcagatta tttagtaaga aaattctggt cttcattttc
     6721 cgaaagcttc gaaagccata agcgactcgt ttcaatgctt tgatgtgggt atgcagattt
     6781 tctattttgg catttgaata aggcaattcg agggctaaac gaatgccgga ttcatagttt
     6841 ataaaagctt tttttataga atgcagttct tttggaacgg aattgggcat cgttcttaca
     6901 agtttaaaga aagtgtccgc ctctttgtcg tgaaacgctt gaagaatttc ttgatagtaa
     6961 aaataggcga ttcgtaattc gtcagagatc gaaagcagac gatccaccac ttcactttcg
     7021 gtgagatacg cccaaccaaa attgcgccat ttggtccatt tgtcataaac aagcttgtcc
     7081 tgtcgtttga tcagaagccg agacaacgcc tttaattgtc gataatattt ggcttgtgtt
     7141 ggatcgtttc gatcaaagct tttcatgatt cgtacgcgaa tatcctcaaa tttacgaatg
     7201 agatgtttga caatgtgaaa acgatccaca acgagttttg cctttggaaa acattccttt
     7261 atcagagagg cataagaaac attcatgtcc gtcacgatga cctttacctt caatcgtgcg
     7321 cgtcgttcaa attgcataaa ataagaaacg agagcctttt tcttccgtga aggaagaata
     7381 tccagtaatt ttccagtatc gccatccata caagaaaagg agtagggacc gacttgattt
     7441 ttcagtgaac gaaattcatc cattaataaa caacgcggta accaatttct ttttactttt
     7501 gtcatgggga tcagagaagc gagtgtccga gaaacggtcg accaagaaac gccgcaatgc
     7561 ttagcaattg tcgtcattgc ttgtttttca gttagctctt ctaaaatttt atagcgcact
     7621 tgtttagcga taaagcagtt tctatcgaca atgtaggtat tcgccgtaaa ataggtgtgg
     7681 cagccacgac agtggtagcg ttgtttcgct aaagcaagat aagtcagttg gttcgcacat
     7741 ttattaagtt gaatgagcga ttttttcttt ccgttcttaa cgatcatgcc ttcatgattc
     7801 atctgtttac acaaaggaca gcgacgaatt tctttactta acgtcgcaga aacaacaaga
     7861 ctcttgatat tcttaatgct cttcgattcc acatttgtga tgaaaatgtt tttatctttg
     7921 attccaagta gtagtttgat atgatgaaga tcaggcatac agaacgctcc tttgattgtg
     7981 ttgtggtgat tcaattctaa cggagcttct gtatgttttg ttttttcatg ctcaaaaggg
     8041 accgatagaa tgattttcta tcagtcctag aaattataga gccgattttg accgacaggt
     8101 ataaccctgc cggtcttttt ttgctgctac aggaaaacta cagactggct acagggaaag
     8161 tacagatgcg cttgccataa taacaatcgt accagccaca aatcgtagtt ttattgcaaa
     8221 ggaggcattc gatcaaatgg aaaaaagcaa ctatcattcc aatgcggatc atcacaaacg
     8281 gcatatgaaa caatccgtgg aaaagcgggc ttttctatgt gcgttcatta tttcgttcac
     8341 agtctgcacg ctgtttttgg ggtggagact ggcttccgta ttggaggcaa cacagatacc
     8401 gcccatccct gcaactcata caggcagcag cactgacgta gtggagaatt tggaggaaaa
     8461 cgctcttgcc accgccaaag aacagggaga tgaacaggaa tggagcctga ttttagtgaa
     8521 caggcagaac cccatccccg cacagtacga tgtggagctt gagcaactat caaatggtga
     8581 gcggatagat attcggattt ctccctatct tcaagatttg tttgatgccg caagaactga
     8641 tggagtttac ccgattgtcg catccggata ccgaacaaca gaaaaacagc aagaaattat
     8701 ggatgaaaaa attgccgaat ataaggcgaa aggctacacc tctgcacagg ctaaagcgga
     8761 agcagaaact tgggtggccg tgccgggaac gagcgagcat cagcttggtc ttgctgtgga
     8821 tatcaatgcg gacggaattc attcaacagg caacgaggtt tatagatggc tggatgaaaa
     8881 cagctatcgt tttggtttta ttcgtcgcta cccaccagac aagacagaga taaccggtgt
     8941 gagcaacgag ccgtggcatt accgatatgt tggcatcgaa gctgccacag agatgtacaa
     9001 ccaaggggtt tgccttgagg aatatttaaa accagaaaaa tgagaaaagg atgttatgtt
     9061 atggacagaa aaagattgac acagcgtttc ccgttcctgc ttccaatgag acgggcgcag
     9121 agaaaaatgt gcttttatgc gggaatgaga tttgacggct gtcgctatgc gcagacgata
     9181 ggggaaaaat cgctttccca tttgctcttt gaaacggatt gcgcattata caaccacaat
     9241 accggatttg acatgatata ccaggaaaac aaggtgttca acttaaagct ggcagcaaag
     9301 accttaaacg gcttattgat aagaccgggg gaaacctttt ctttctggtt gttcctgcat
     9361 acgccattga caattatcca gcgcagaggt cacgaagtaa aggagtttcc agagccaaac
     9421 agtgacgaga ttaaaggggt ggatgcaacc atttcagagg gctggattga tttaaaagtg
     9481 cgaaacgata ccgactgcac ctaccaaata tgggtgacct tagatgatga gaaaatcatc
     9541 ggtcaggtgt tcgccgacaa agagccccaa gcattataca aaattacaaa tggcagtatc
     9601 cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc aggttaaacg gatgcaagtt
     9661 gccttaggta ccggggaaat aatagattgc aagctgcttt atacaaacaa atgcaaaatc
     9721 tgttatcccc tcccggaaag tgtggatatt caggaggaga accaatgaga aaaagtgtgg
     9781 gcattactgt ttttggatgc gaacaggatg aggcaaatgc tttccgcgct ttatcgccgg
     9841 attttcatat tatccctacg ctgattagcg atgcgatatc ggcagacaac gcaaaattgg
     9901 ccgctggcaa tcaatgcgtt agcgtaagcc ataagtccga ggtttccgag gcgacaattc
     9961 ttgcgctgag aaaggtcggg gtaaaataca tttctacccg cagcatcggc tgcgatcaca
    10021 ttgatacgac tgccgccgag agaatgggaa tctcggttgg cacggttgcg tattcgacgg
    10081 acagcgttgc ggattatgct ttgatgctga tgctgatggc catacggggt gcaaaaccca
    10141 ccatgcacgc cgtggcgcaa caagatttca gattggatcg tatccggggg aaagaactgg
    10201 gggatatgac tgtgggagtt attggaaccg gccatatcgg gcaagcggtc gtcaaaaggc
    10261 tgcggggatt tggatgccat gtgctggcct atgataacag ccgaaaaatg gatgcagatt
    10321 atgtccagct tgatgagctt ctaaaaaaca gcgatattgt tacgctccat gtgccgcttt
    10381 gtgcggatac ccgccatctg atcggtcaga agcaaattgg agagatgaag caaggcgcat
    10441 ttttaatcaa caccgggcgc ggagcgcttg tcgataccgg ggcgctggtg gaggcgttgg
    10501 aaagcggaaa gctgggtggt gcggcactgg atgtgttgga gggcgaggat cagtttgttt
    10561 ataccgattg ctcgcagaaa gtgcttgacc atcccttttt gtcgcagctt ttaaggatgc
    10621 cgaatgtgat catcacaccc catacggcgt actacactga gcgtgtgctg caggatacta
    10681 cagaaaaaac aatcaggaat tgtctcaatt ttgaaaggag tttacagcat gaatagaata
    10741 aaagtcgcaa tcatcttcgg cggttgctcg gaggaacatg atgtgtcggt aaaagccgca
    10801 atagaaattg ctgcgaacat tgatacggaa aaattcgatc cgcactacat cggaattaca
    10861 aaaaacggtg tatggaagct atgcaagaag ccatgtacgg aatgggaagc cgacagtctc
    10921 cccgccatac tctccccgga taggaaaacg catgggctgc ttgtcatgaa agaaagcgaa
    10981 tacgaaacac ggcgtattga tgtggctttc ccggttttgc atggcaaatg cggggaggat
    11041 ggtgcgatac aggggctgtt tgtattgtct ggtatcccct atgtgggctg tgatattcaa
    11101 agctccgcag cttgcatgga caaatcactg gcctacattc ttacaaaaaa tgcgggcatc
    11161 gccgttcccg aatttcaaat gattgataaa ggtgacaagc cggaggcggg tgcgcttacc
    11221 taccctgtct ttgtgaagcc ggcacggtca ggttcgtcct ttggcgtaac caaagtaaac
    11281 ggtacggaag aacttaacgc tgcgatagaa gcggcaggac aatatgatgg aaaaatctta
    11341 attgagcaag cgatttcggg ctgtgaggtc gggtgtgcgg tcatggggaa cgaggatgat
    11401 ttgattgtcg gcgaagtgga tcaaatccgg ctgagccacg gtatcttccg catccatcag
    11461 gaaaacgagc cggaaaaagg ctcagaaaat gcgatgatta cagttcccgc agacattccg
    11521 gtcgaggaac gaaatcgggt gcaggaaacg gcaaagaaag tatatcgggt gcttggatgc
    11581 agagggcttg cccgtgttga tctttttttg caggaggatg gcggcatcgt tctaaatgag
    11641 gtcaataccc tgcccggttt tacatcgtac agccgctacc cacgtatgat ggccgccgca
    11701 ggaatcacgc ttcctgcact gattgacagc ctgattacat tggcgttaaa gaggtgataa
    11761 gtatggaaaa tggttttttg tttttagatg aaatgttgca tggcgtccgt tgggatgcca
    11821 agtacgccac atgggataac ttcacaggaa aaccggtgga tgggtatgag gtgaatcgca
    11881 tcatcggcac aaaggccgtg gcgtttgctc tgcgcgaggc acaaatccat gcggctgcgc
    11941 ttggctatgg cttgctttta tgggatggat atcggccaag aactgcggtg gactgcttcc
    12001 tgcgttgggc agcgcaaccg gaggacaatc tcacaaaaga aaaattttac cccaatatag
    12061 agcgagccga gttgattaca aagggttatg tggcctcaca atccagccat agccgtggaa
    12121 gcgcaattga tcttacgctc taccacctgg atacagggga acttgtttca atgggaagta
    12181 acttcgattt tatggacgaa cggtcgcatc atacagcaaa agggataggg gatgcagagg
    12241 cacaaaatcg aagatgcttg cgtaaaatca tggaaagcag cggatttcag tcttatcgct
    12301 ttgaatggtg gcactataag ttgattgatg agccataccc cgatacctat tttaattttg
    12361 ccgtttcata atgaaagtat tggttttgta tcagattttg gatttgataa aatctatgct
    12421 ggtcatggac aatcttttac tggaaagtgg ttaaagcatc ttttgtaata cattgcaaat
    12481 tatagtattc aatctgccca gcggcagaaa agaaaaaaac cgctgttggg cagacctttc
    12541 ccacgcccat tttgtatttt gctggcggcg gttttgagaa atcaaggccg ccgttctttt
    12601 ctggcagcaa aaggtgtggc ggcccatagg aggatgccgc tatgctgttt tgctattgcc
    12661 atgctctaat ccacttccac cactcaaaaa aagccttgcc cctc
//
LOCUS       DAYYAJ010000092        12676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000092 DAYYAJ010000000
VERSION     DAYYAJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.092"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..184)
                     /locus_tag="ACHATZ_002102"
     CDS             complement(<1..184)
                     /locus_tag="ACHATZ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404616.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDE"
     gene            complement(178..579)
                     /locus_tag="ACHATZ_002103"
     CDS             complement(178..579)
                     /locus_tag="ACHATZ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8404617.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(766..2574)
                     /gene="uidA"
                     /locus_tag="ACHATZ_002104"
     CDS             complement(766..2574)
                     /gene="uidA"
                     /locus_tag="ACHATZ_002104"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8404618.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2597..4363)
                     /locus_tag="ACHATZ_002105"
     CDS             complement(2597..4363)
                     /locus_tag="ACHATZ_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8404619.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4376..5170)
                     /locus_tag="ACHATZ_002106"
     CDS             complement(4376..5170)
                     /locus_tag="ACHATZ_002106"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8404620.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5180..6556)
                     /locus_tag="ACHATZ_002107"
     CDS             complement(5180..6556)
                     /locus_tag="ACHATZ_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8404621.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(6676..7323)
                     /locus_tag="ACHATZ_002108"
     CDS             complement(6676..7323)
                     /locus_tag="ACHATZ_002108"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8404622.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(7407..8834)
                     /gene="uxaC"
                     /locus_tag="ACHATZ_002109"
     CDS             complement(7407..8834)
                     /gene="uxaC"
                     /locus_tag="ACHATZ_002109"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8404623.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8908..9987)
                     /gene="uxuA"
                     /locus_tag="ACHATZ_002110"
     CDS             complement(8908..9987)
                     /gene="uxuA"
                     /locus_tag="ACHATZ_002110"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8404624.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9990..11621)
                     /locus_tag="ACHATZ_002111"
     CDS             complement(9990..11621)
                     /locus_tag="ACHATZ_002111"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8404625.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11925..12596
                     /locus_tag="ACHATZ_002112"
     CDS             11925..12596
                     /locus_tag="ACHATZ_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8404626.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         4080 a         2517 c         1649 g         4430 t
ORIGIN      
        1 attcatctac ataggtaacg aattgaccta cctcgttaaa tccatttgtc aaacataact
       61 tagccatata agaatgataa tgtttgagta tcatcttgat cgaaattgga tctccttcta
      121 gagcagataa gataatggat agtggaacta ataggtactg ccgcttttta gatttatcta
      181 ccataggtca taccccattt tcaattgttc aactgccttc tttcgttgat accagatacc
      241 acttgtgctc atattgagaa ttttaccgat ttcagcatca ttaaaacctg caaaataata
      301 aagtaggata atctttcttc ttatctccga taactgatca attgctgctg ataacttttg
      361 atctgaaatt aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt
      421 ttgggaatac ctgccttcat ttgaatctgc taatggttcc tcattatcct ttatgttagc
      481 gtttcgtttt cgaatagact tagcttcgtt tcgtaatact tttttacagt aggcatcaaa
      541 ttggtgttgt atagatatgt cattgtaatg ttgttgcatg ataatatgct cctttttccc
      601 cctttccact aatacaacaa tcagaaagcc cctttttttc gaaaaaacta gaaaaaaaac
      661 ataaaaatat ttatcattct ttaaacagaa aatctttcta taaaaaacaa aaaaaaacga
      721 ttaggctagg ctaatcgttt tttgaacatt cttaaaaaat ccaaattatt ttttataact
      781 gaaattgggt attgatgacc atcgtttctg tagatagtaa gcaatcattt taggttttct
      841 atctctagta aagattcctt tcttatttcc ttgcactctt aaaatccctt gacttgttgc
      901 aaaatctgca aaattccaca cttgttcacc aacaaaattt tcacatgtat ctattacttc
      961 gtgatttgct tcgtaataag ccacttgata ctcttctgta aacattgttg gtactgtatc
     1021 atgaaatcca gctactgtat ctgctccata ttctgtaaac attattggcg tatcaggaca
     1081 tctttccatc catccatcta gctctttttt caacagatat tttgcatgtg ccagatcccc
     1141 tcccgaaaca taccaaccat aataacgatt taaacataaa acatcgacaa tatctcctac
     1201 cttacaaaca tctggcgttg attttagata agtaacgatt gttacaggtc ttttctgcgg
     1261 atcaagttct ttagttaatt caactaaagg ttcaaaatat tctttagcac cttcggaatc
     1321 cgaatctggt tcatttgcaa tagaccacat tacaacactt gggtggttct tgtcgcgttc
     1381 tatcaattcc ttgatcaccg ttttatgaga attttctgtt cgtatctctt tccacgtatt
     1441 atgtttttcc acattatcat ctaataaagt ggccatgaaa tttaaatgta accctactgc
     1501 aggtacttca tcaataacta caataccttc tcgatctgct aatcgcataa tttcttctga
     1561 gtaaggataa tgtgatgttc taaaagaatt agcacctatc catttcatta aattaaaatc
     1621 tttaatattt acagcctcat tcaaaccacg tccattaaca tacgaatcct catgcttacc
     1681 aaatccttta aaataaaaag gtttattatt aatcaaaaat ttgccattgg taacctgaac
     1741 actgcgaata ccaaattctt ctgtataggt atcgatcact ttattatcat caattaaact
     1801 aattttcata ctatataaat aagcgtctag aggttcccaa aaaattggat tcttaagctg
     1861 aatcattcct ttctttcctt cagcttcagc tacaagttga gcctcttcgt catatatcgc
     1921 tattaagatt tttccggtcc attctgtatc gatattgaat tctacacttg catccaaatc
     1981 agttattctt gtattaatag taatatcact tatatagtct ttcggagttg tatataactt
     2041 aacaggtcta tgaatacctg cataattgaa gaaatcaaaa ttaactaagt tttccttttt
     2101 tatatcccca ttttcctgta ctgtttcctt cataatcccc acaggtaaag tactatcgtc
     2161 aataatattg tttacagcaa ctgtaattcg attattcccg tcttttacaa accgattaat
     2221 ttcagactca aatggtgtaa acccaccttt gtgttgagta ataaactttc cattcaaaaa
     2281 aacttttgct tcatgtgttg cagatccaaa acgaagaact aatctttctt ctaataatct
     2341 tttaggaaga gtcacttctt tttcatacca cacccatcca acatgatttc taatgttctc
     2401 tgtttcaaat aaatcattat aagaagaggg aactgaaatc ggataactct ctttttcatt
     2461 tattggttct aaagcaagtt cttcctgtaa gtcatcattt gtatctaatt taaatctcca
     2521 gagaccgctt aagtcaatta cttgcctcgt ctctgtcata atgggataca acatataaac
     2581 cactcctatc taaatattaa ataaatgtat cctttaaacc acaataagca tctattggac
     2641 ttactccctt aaattcagaa agacccatta gtttgtccac caatatttct aaagtttctt
     2701 gttttccatc ataagtatta atataagtct ttacttgagg tacatcagcc aaatgaaacg
     2761 gacactgtac agatacaaaa attgttggaa tttcatgaat gtaaaacggt atatccggag
     2821 ttcctttact tgctggccag acaattcgtt gaactgtgcc tggattaaca ttagctatat
     2881 taatgatcaa gtcatatttt ttagttaaat ttttaattgg tctcttagca gcatatattc
     2941 cagctatatt tttaattttt tcttcttgcg ataatccttt caattcttct tcaggtgaaa
     3001 tgtatttttc aacttcaaat ccttttgctg acaaaatctg tttcaataaa tcaatggctt
     3061 gaggtctatt tccagcaact acttcaccaa acccaccttt taccccatgt acatctacta
     3121 gtaaaatcct tttatatttt tctggtacaa taggccaaat atcttgttta tctttaacta
     3181 atgtaattgc ttggtcagcc acctctttaa aaattgattt attttcaatt aaaccaattt
     3241 tttccagagc ttcttgctta ggtaataaaa tttgttcttt tgcttttttg tgtaatccca
     3301 atttagcttt taaccctaaa attcttttta aggcatctaa taaccgatca tcagtgataa
     3361 ttcctttacg atagccttcc atcatataat taaagtcttc atctggatca ttaaaaaata
     3421 gaaatagatc acatccagct gcaatagaag tgggcaataa ttctgatcgt ttcattgccc
     3481 cagtcattcc taacatatga ctagcatcag taactactaa tccattaaat ccgagttttc
     3541 ctctaagtaa gtcagtgatt aattcttttg ataaagttgc aggaagtaaa tcttcatctt
     3601 tcagatcagg attaaaatgt tttgaataag ctggcatatg gatatgacct gccataattg
     3661 aaggtaatcc agcttctatt aagcctgagt atacttttcc aaaagaatta tcccattctt
     3721 ccgtacctaa actattaata ctaaaagata gatgctgatc tcgttcgtca ataccgtcac
     3781 cagggaaatg tttagctgca ggcaaaattc cactctcctg aatacctttc atataagcta
     3841 aactaagttc taaaacttta tcagggtttt gtccaaaaga tcgactagaa atgataggat
     3901 ttctccaatt tctattaata tctacaatag gagcaaaact ccagttacat ccaatagccg
     3961 atgcttcaat cccagaaact ctccctaatt cataagcata tttcgcatca tttgttgcac
     4021 caatttttac ttctacacca acttcagtac catctgtaca agctccgtta ccacctgctt
     4081 ccgtattagc agcaattaat agaggaattt tactttctgt ttgtaagata tagttttgat
     4141 catatacctc atcagcagtt cctggattat atcgtacagc tccaatatgg tactgattga
     4201 ccatattact caagtaattt ttatcacgac tagatcccat attaacaaac aattgaccta
     4261 ttttttcttc caaagtcata gaatcaatcg tctcttcaat ccaagaaata gcatcttcat
     4321 ctaaattata tggttttttt cgtaaatcta cttttatagt cattttcttt ctcctctact
     4381 tattagtaag atttttccaa aaagtatcta tatatttttg ttcaatatct acataatctc
     4441 ccgataaaca tttaaataaa acatcagttc ttaagttttc ccatgattct ttttctttat
     4501 tgaccaaaat aggataaaag aaaactttat ttttatttgc agcttctaga tcaccaggag
     4561 aatcaccaat cattaatatc ttatccaaat cacaaccatt ttcaattaca cttcgaataa
     4621 tatcttcttt ttttccttta tcttgacaac acagttcatc aactaatgat aataattcat
     4681 gtctttccca ttcttcttga acagcttcct tattagctga gcttacaata attatcttcc
     4741 catatttttt cagagctttt atagtatctc tagcacttaa aaagggttta tctaatcctt
     4801 ttgcttgact aatctgttga ttgacttctt ccgaccattt taaagctttt aataaatcct
     4861 ctgactgaga atattctatt tccctcttca aagatggatt agacaattct tttgttagag
     4921 atacccagtg ctttaatcga ttaatcgatg ttacatcatt tttttctaat gttttaacta
     4981 aacctaaaaa tctgtttatt ccacgagtct ctgaatataa attaatatta ttccaatcct
     5041 gtaggaattt ttctctatga tgaacatcaa aaatctctgc agcaattggt ccgaaaaaga
     5101 gattgtgttt ataagtcatc gtatccataa cacagccatc tgaatcaata caaaatatcc
     5161 aattattcat atttctctcc tatttatgtt tttgttttaa aagctcgcaa ttttctaaga
     5221 ctttatcttt agataatgga taaaggaaaa ataaaattaa tcctacaatc agaaaacaaa
     5281 ttgatggaat taatgttgaa gcgttatata acttttctaa aacatctccc gtttgtacat
     5341 ttgctaaaga gtcatagcct ataatagaaa gagtccaacc tccgacgcct ccagctagag
     5401 cttgacctat ttttctagca aaagaatata ccgcatatac ggtcccatct tctctttgat
     5461 cagactttac ttcttgatca tcaataacat cagtaatatt tgcccaaata acagtattaa
     5521 acacacccaa acccatatag ccaaccgaac taagaattaa gaatacatac atattactag
     5581 cttttatcaa aaataagatc aaatatacta ttgctgaaaa gaacattcct actgatgcca
     5641 gttctttttt tccatatttt ttacttaaaa acaaagttaa aggcgaaaca actattaaaa
     5701 ccaaaatagg attgataaaa ttcattaaaa ctattccctg tgcatcatta tagtaatcag
     5761 gaaatacata attattcata ctcataatta ataattgagc aaggagtaag aaaatagccg
     5821 caccaattat agaaagtaat gccctatttt taactaaccc ttttaaagat tttaacaatg
     5881 aaactttgta ttgagattgg tcaattctta ctcgttcagt agttagttta taacatatta
     5941 aataacaaat aagtgcacat atcgaaaata ttcccgcaac aattgtaaaa gttgatccac
     6001 cacgaactat ttgagcgcct gaatcatctc ttgtataaat aaataatggt gttaatacac
     6061 caatgaccaa acttgctaaa gtagctccaa catttcggaa tgtcgatagc attgttcttt
     6121 cttctgtaat agaagtaata gctgatgcca ttgatccata aggaatattt atagatgtgt
     6181 aaaaaatact cccccataat atatacgtaa catacatgta tactacctta acgctcatag
     6241 aagctgaaat catagaagtt tgatacatta aaaaacttgc taaagcgact ggaccactca
     6301 ttcttaaaat ccacggttta aacttaccct gcttagttgc tttcgatcta tcaacaatac
     6361 gccccatagt tacatcagta actgcatcga taaatcttgc tactaaaaat aatgttccaa
     6421 cggcagctcc gcttatcccc aatacttttg tataaaagat cattaaaaaa gaactagcaa
     6481 aaatgaaagt aaaatcgttt ccaaaatctc caaacatata tcctattttg tctcttatac
     6541 caaattttct ttccatacaa ttctctccct tctgttgtta agcgttttca ttctgatgaa
     6601 agtacgtact atactctgaa gcgtaaagag tcagtattta ttttttacat ttcagagtat
     6661 tatttgtttt ttctcctaat aattctttat ctcataaaac ttatctatat atttactagc
     6721 tactcgactt acttcttcaa aatcaccttt ttcaacagaa gccaataaat tcccacctac
     6781 accaactgca acaactccta cttcaaacca atcttctaag ttctctatac ttactccgcc
     6841 agtaggcatt atatttacgt gtggtaatgg agcttgatag gcttttacaa tggatggacc
     6901 aaaagcagaa cctgggaata gtttaatgat atctacgcca gcagtaagtg cctcctttat
     6961 ctcagtaatt gtcatacaac ctggtaaata aggaatttga tataaattac aaatctcagc
     7021 tgtttgtcga tcaaaagatg gactaacgat atattctgcc ccagccataa ttgcgatacg
     7081 agcagtaaac gcatctaaaa ccgtaccggc tccgattatt acatcttttt gatcagcata
     7141 ttttttagca atttctttga ttatttcttc agcattagga actgtaaaag ttagttcaat
     7201 ccctttaatt ccacccgcaa taattgaatt tattgcttta atggcctcat ccttattttt
     7261 cccacgtaca accgcaatga ctccagccgc ttctaactta cttaaaattt ctactttttt
     7321 catatctatc tccttccttt atggatacat actttgtcta gctgtactaa cttacaaaga
     7381 ataaatttaa gcttttattt caagtattat tttctacttc cgatttcaaa aaaattaaaa
     7441 taattatacg cattacgata acaaatattc tccactatct gactaagata catttcatca
     7501 tctggaattc ggcctgcttt aacccactcg cttagtaatt gacataatac tcgacgaaaa
     7561 tactcatggc gaggatagct tagaaaactt cgagaatcag tcaacatacc tgtaaaattc
     7621 gacaacaaac tttgttgtgc catcgtagtc aactgttgac gcattccttc agctgtatca
     7681 ttgaaccacc aagcacatcc caattggatt ttttgaatca tacccccttg gaagctttgc
     7741 attgctgttg ctagtggaag ccaatcagtt ggatttaatg aatataaaat tgtttttggt
     7801 aagcacccac tcttttcagc ggtatcgaac aatgctacaa cattttgtgt caaactagct
     7861 tgatccccca tcgaatcacc accgacatct actccaattt gttcctttaa tatacttgaa
     7921 ttatttctta aagcatttaa atgcatttgc atcgtccaac catatcttgt atactcagac
     7981 atcaaatgta attgaatacc tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt
     8041 tttcctgcta aagcttttat taacagattg tcgagtattt tttcatctgc tatttcataa
     8101 aaataagtat tcaaaccgtg atctgctaat cgacagccaa tttgatgaaa atgatccact
     8161 cgttggctca atgcttgctt caacccatta aagtctttga ttggtatagc cgctgtttga
     8221 ctaagtttta aaatatagtt cgaaaagcct gatgatttga tactccagac cttatctgga
     8281 cggaaagtag gtaatacctt gaaagaatta tcctgtttag aaagctcagt atgataccac
     8341 aagtcatctg taggatcatc cgttgtacag atggctttca catccatcat ctccaacaat
     8401 tctttcgggc gataggaatc cgtttgaatt ttttcgttag cacgtcgcca aatttctggt
     8461 gcattcttct cagaaattgt caaatcaata tcaaagtaac gttgtaattc caaatgagac
     8521 cattcaaaaa taggattacc tggcgccttc tccactgttt ttacaaaagc taaaaatttt
     8581 tcataatcat cacctggacc actgatcaac tcttcttcta taccattagc tcgcatcaat
     8641 cgccatttat aatgatctcc agctccttta tcatttaacc atacctgagt caaattcttg
     8701 tattgcttat tttctagcaa atatgctggg tctaaatgac aatgatagtc gataattggt
     8761 tggtgttttg caaatttatg aaaaagtctt ttagctattt cattctctaa taaaaagttc
     8821 ttatctaaaa acatatttaa acactccttt ccttccgtta gtttttagaa gagaaaattt
     8881 ttcctctaaa taaactccct tatataatta tttactcgcc cccttttcaa gcgcttccca
     8941 aataccattt aaataaactg cacctagagc tcggtcatat aatccataac caggtctacc
     9001 ggtttctccc caaatcatcc gaccatgatc tggacgaata tagccattaa atccattatc
     9061 gtacattgtt tttaatatct tatacatatc tagagaacca tattctgatt tatgcggcgc
     9121 ttcgtaaaaa tcgagtccat tgtcacttaa atatttgata ttacgaacat gtgcaaaagg
     9181 aatccgatcc cgttgacaaa attcagatag aatttcatag acatcattat ctggtcgttc
     9241 tgcaatactt ccagtacata atgtaatacc attagaagga gaatccacta cattacacaa
     9301 ccaatctaaa tcgtcacgat ttttaacaat acgaggtaat ccgaaaatgg aatatggtgg
     9361 atcatctggg tgaactgcca ttttaatccc aacttcttca catgtcggga taattgcttt
     9421 gagaaaatat gcaaagttct ctcgtaattt ctcttcatca acttctttat aggcttctaa
     9481 taattgctta atttgtgata accgatgagg ttcccaaccc ggcaattcaa aattattagt
     9541 agaattttct acttctctaa taatttcctc cggatcagta gggatatttt ttgaaatata
     9601 ggctaacgta gaagacccat catctaaacg ataatccaaa tccgatttta cccaatcaaa
     9661 tactggcata aaattataac agataacctc aatcccaaat tctgccaaat tgcgaatcgt
     9721 ctgtttataa ttctcaatgt attgatctcg tgttggtaaa ccaattttga tatcatcatg
     9781 aacattaaca ctctcaataa ctttcaaggt aagtccattg gcttcgactt catcttttaa
     9841 agatttaatt cgttctctcg gccaaacctc tccggctggt atatccatta atgttccaac
     9901 aattcctttc attgctggga tctgacgaat ttcttgtagt gagatttttt cttcatttgc
     9961 cccataccat ctaaaagtca tttccatagt tactttcctc caacttctgt tatatattta
    10021 ttgattgttt tacgaactgc ttgaggacct actagcattt cgcaaaagat tttttcagtt
    10081 ttctttccta ccccaacttg atataaatct aaaccaaaga tttctttatt ttctaaaatt
    10141 tcctgtaccg cacaatgaat ctttgaagga tcttgttcac ctaatttcaa tactgataat
    10201 ttatcctgca actctaccaa taacggatca ggacttggca caaaattttc cccttgatca
    10261 tcgactgcca gtaaataacg taaccaagca gcaatagcga taggaataaa tgttagattt
    10321 tctgcatttc cgtctttttc gacatacttt ttaattgttt caccatagcg aatcgctact
    10381 ttttgagaag tatctgttgc aattcgttgt ggtgtatctg gaatataggg attaggcaaa
    10441 cgtttattaa ttacttcatc aataaattgt tttggatcaa taatgcctgg attttcaact
    10501 actggtaaac cttctaaata acctattcct ttgattaaac caaccaaatc atcatcctgc
    10561 atctctgctg aaattgattg atatcctagc agacaacctg ttactgccaa agcagtatga
    10621 agtggattca agcttgtcgt aaccttcatg atgtctgctt tatcaaccgt ctctctatct
    10681 gtcataatca caccagcctt ttccaattta ggccggccat ttggaaaact atcttcaata
    10741 actaaataat gaactacctc agtattagtg aaagcagcaa tattggtatg tttttttgtg
    10801 tgaatgatat ctaaatcctc gagccccatc atcaatagat cttcctttac attctctgaa
    10861 ggatttggcg taattcgatc aatcatactc caaggaaaag tcactttttc tttatccttt
    10921 aaataagcaa taaatttttc agaaacatat ccttcttttt gccaagcttc tgcaatcgta
    10981 aaaatactat cttgaaaacg ttgaccattc tgtgagaaat tatcagtaga aaccatcgca
    11041 atcggcaact ctccagcatt aaatctgcta tataacaagg atgttacaat actcatcgta
    11101 tgttgaggat tgctaggtcc agatttgata tcagctttga ctaccgaaaa gaattgccct
    11161 ttagaatctt ttaaactgta acctttttca gtaatagtaa acgttgtaaa ctgtaattta
    11221 ggatcttcaa aatatttaac cactctttga tagtccgcta tattcttcgg attgcaatat
    11281 aaactatcag ccgttactgc caaaagtctt tttttgagtt caccatctcc actcataaca
    11341 atttccaaaa tgtcattgtt atagtcatga taagcttttt ccactacttc ttcatcaaat
    11401 gtttcacaca caactactcc tgtttctaat tcattttgat ctgctaagca ttgagcaatt
    11461 tctgcatgaa atcctcgata aagatttcct ccaccaaaat gaagccatac tggatgttgc
    11521 tggccttctt gcttaacttc tttgacatca aaacacggca gtagaatctg catttcattg
    11581 aattcttcga tagaattttg ccaattttta atcgaaacca taattaacct cctcaaactg
    11641 atatgttagt tttgatacat tttatatttt aaaacccatt gtaaccgttg tcaatataaa
    11701 aagggaataa acttgctttt ttctaccata tcagaatgat tgtatttttt attcagaaaa
    11761 ataaaatact ccttcttttt ggactgattt tatcgaaact tattttttct ttttttcatg
    11821 tattctttta tttttataac tagtgtatta cttcaaaatt tgatattata tggcatgaac
    11881 agaattaaaa tatcagttaa aataaaagtt aaggagcatt gtgtatgaca attaaaacta
    11941 gtctccaaaa tcaagcttac caatctattc gccgacaaat tatctatgct gacttagaac
    12001 ctggaaaaaa agtatcagaa aaaaaattag aagaaacact ttctattggt cgtacaccaa
    12061 tacgtgaagc actgattcaa ctccgacaac aaggtcttgt tgatacaatt cctcaaagcg
    12121 gaaattatat ctcattaatt gatttagatt cggccaaaaa tgctcgattt gtcagagaac
    12181 atttggaacg acaaattatg ttagagtgtt gtgccaaaat gaataaccaa aaaaaacgga
    12241 ttttagaaac tataatttca gaacaagaaa aagctgttag tcaaaaaaat gctcgtagtt
    12301 tttttcataa tgataattta ttccataaag cttgttttga aattgcagag cgagaagaag
    12361 tatggcaatg gttagaaaat aacaatacac acttagaacg atttcgatgg ctacgagtaa
    12421 aaacggaagg attaaagtgg gaaaaaatta tggaacaaca ttatcaacta tttgaggcat
    12481 taacaaaaca agatccagaa gaagctaact ttctaacatc gttgcatctt catttaatgc
    12541 ttaatgagca aaatattgtt ttagctactc atccaaaata tttcaaaaat atataacttt
    12601 taactctttt taaattaaat ggttgttctg atcagaaaaa tatcgcttgt tttcttatta
    12661 tgtaatatat ttcata
//
LOCUS       DAYYAJ010000093        12452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000093 DAYYAJ010000000
VERSION     DAYYAJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.093"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..812)
                     /locus_tag="ACHATZ_002113"
     CDS             complement(6..812)
                     /locus_tag="ACHATZ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404627.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            950..2464
                     /locus_tag="ACHATZ_002114"
     CDS             950..2464
                     /locus_tag="ACHATZ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8404628.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGEG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2867..3850)
                     /locus_tag="ACHATZ_002115"
     CDS             complement(2867..3850)
                     /locus_tag="ACHATZ_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8404629.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3855..4502)
                     /locus_tag="ACHATZ_002116"
     CDS             complement(3855..4502)
                     /locus_tag="ACHATZ_002116"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8404630.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4587..5219)
                     /locus_tag="ACHATZ_002117"
     CDS             complement(4587..5219)
                     /locus_tag="ACHATZ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8404631.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="ACHATZ_002118"
     CDS             complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="ACHATZ_002118"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8404632.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5783..6520)
                     /locus_tag="ACHATZ_002119"
     CDS             complement(5783..6520)
                     /locus_tag="ACHATZ_002119"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8404633.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6517..7506)
                     /locus_tag="ACHATZ_002120"
     CDS             complement(6517..7506)
                     /locus_tag="ACHATZ_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8404634.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7764..8306
                     /locus_tag="ACHATZ_002121"
     CDS             7764..8306
                     /locus_tag="ACHATZ_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8404635.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8323..9408
                     /locus_tag="ACHATZ_002122"
     CDS             8323..9408
                     /locus_tag="ACHATZ_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404636.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9420..10226
                     /locus_tag="ACHATZ_002123"
     CDS             9420..10226
                     /locus_tag="ACHATZ_002123"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404637.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10227..11054
                     /locus_tag="ACHATZ_002124"
     CDS             10227..11054
                     /locus_tag="ACHATZ_002124"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404638.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11051..12124
                     /locus_tag="ACHATZ_002125"
     CDS             11051..12124
                     /locus_tag="ACHATZ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8404639.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3896 a         2247 c         2569 g         3740 t
ORIGIN      
        1 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
       61 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
      121 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
      181 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
      241 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
      301 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
      361 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
      421 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
      481 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt
      541 aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt
      601 ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc
      661 agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata
      721 acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta
      781 ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt atatccttaa
      841 ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac agagttgata
      901 ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt
      961 agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg gcgcagatca
     1021 gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat catcaggcgc
     1081 ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg tcgtgattat
     1141 tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg ggttgaatcc
     1201 agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat caagcggtat
     1261 gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga atgatgcctt
     1321 ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc ttcgatactt
     1381 tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca aactggacaa
     1441 cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg atggctcggc
     1501 tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg aaatccctat
     1561 ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca atgaagtgac
     1621 tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac
     1681 aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt tagctgatta
     1741 tattacgaca aatcaagaag gatcaactgg agagggtatc accatgattg aaaaattagg
     1801 tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc aggatgaggg
     1861 ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa
     1921 aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta cagcattgcc
     1981 tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca cagctattga
     2041 attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat tagcagaaaa
     2101 aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata cggatgttgc
     2161 ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact tatcgtctcc
     2221 gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg gtgtgaaaat
     2281 caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt tatatgctgc
     2341 tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt cagttgcgga
     2401 gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt cggaaaatga
     2461 atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct aataccgagg
     2521 gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta
     2581 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg
     2641 cagatgcaag cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa
     2701 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa ttccttcgga
     2761 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     2821 ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat tcagttatct
     2881 tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct tgtttgactg
     2941 cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct agaaaaggcc
     3001 cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt ctaggtgtca
     3061 ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct atctcaggca
     3121 cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct atagaagcta
     3181 aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact ccccgacctt
     3241 cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc aattcatctg
     3301 caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg atgattttcg
     3361 cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt gctcctgcat
     3421 ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag
     3481 tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga
     3541 ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt
     3601 atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact acctctccag
     3661 ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa
     3721 tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt
     3781 taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa
     3841 tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg
     3901 ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca
     3961 agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt
     4021 aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa
     4081 tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc
     4141 atggactcag gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga
     4201 gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc
     4261 agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct
     4321 tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt
     4381 tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga
     4441 gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc
     4501 atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa
     4561 aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt
     4621 aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca
     4681 cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact
     4741 ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc
     4801 aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt
     4861 ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac
     4921 gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa
     4981 aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat
     5041 gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga
     5101 atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct
     5161 aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata
     5221 agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc
     5281 gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt
     5341 ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg
     5401 ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc
     5461 gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg
     5521 tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg
     5581 attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat
     5641 tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg
     5701 gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca
     5761 gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca
     5821 atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat
     5881 agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt
     5941 tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta
     6001 atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt
     6061 ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag
     6121 tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag
     6181 tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga
     6241 ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta
     6301 aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt
     6361 tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat
     6421 cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga
     6481 ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac
     6541 tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc
     6601 cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca
     6661 taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt
     6721 tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa
     6781 agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac
     6841 tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa
     6901 ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc
     6961 ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt
     7021 actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg
     7081 acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc
     7141 ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata
     7201 ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc
     7261 ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg
     7321 agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata
     7381 attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg
     7441 cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc
     7501 gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt
     7561 ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa
     7621 ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc
     7681 ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag
     7741 aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc
     7801 aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt
     7861 cccagttgga acgagattta agttcaccat caatggaaac attcttttct attcttgaag
     7921 tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt
     7981 attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc
     8041 tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag
     8101 gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg
     8161 aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt
     8221 atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga
     8281 ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt
     8341 tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt
     8401 gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg
     8461 atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg
     8521 aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat
     8581 gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa
     8641 atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct
     8701 ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt
     8761 gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt
     8821 gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga
     8881 atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt
     8941 tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag
     9001 ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg
     9061 atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg
     9121 cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca
     9181 gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa
     9241 atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa
     9301 gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac
     9361 gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa
     9421 tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga
     9481 tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc
     9541 tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg
     9601 tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt
     9661 ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg
     9721 tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga
     9781 atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt
     9841 ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat
     9901 tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg
     9961 agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag
    10021 cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg
    10081 tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg
    10141 gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg
    10201 agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag
    10261 ggatcgtctt tttattctta tatgcaccga tcttctatct gattttttat tccttcaatg
    10321 aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg
    10381 aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat
    10441 tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac
    10501 gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta
    10561 tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg
    10621 tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat
    10681 tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc
    10741 aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta
    10801 tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct
    10861 ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca
    10921 atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta
    10981 ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg
    11041 cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt
    11101 gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact
    11161 taccatctat aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga
    11221 atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat
    11281 ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat
    11341 gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat
    11401 tgatccacga tttttagact tggattttga tccagataat cagtattcga ttccatattt
    11461 ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca
    11521 ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg
    11581 agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc
    11641 acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt
    11701 ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc
    11761 tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga
    11821 aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg
    11881 tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta
    11941 tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc
    12001 ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct
    12061 aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga tgtataggag
    12121 ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt
    12181 cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt
    12241 gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt
    12301 aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag
    12361 taattggaaa ctatgcaaat atacttaacc cttctgtaat ctattaataa aactaatata
    12421 tgaatccagt tcaatatttg tagctatgaa aa
//
LOCUS       DAYYAJ010000094        12177 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000094 DAYYAJ010000000
VERSION     DAYYAJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.094"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..303)
                     /locus_tag="ACHATZ_002126"
     CDS             complement(139..303)
                     /locus_tag="ACHATZ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404640.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            598..1926
                     /locus_tag="ACHATZ_002127"
     CDS             598..1926
                     /locus_tag="ACHATZ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8404641.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2128..2961
                     /locus_tag="ACHATZ_002128"
     CDS             2128..2961
                     /locus_tag="ACHATZ_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8404642.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2977..3714
                     /locus_tag="ACHATZ_002129"
     CDS             2977..3714
                     /locus_tag="ACHATZ_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8404643.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3869..4837)
                     /locus_tag="ACHATZ_002130"
     CDS             complement(3869..4837)
                     /locus_tag="ACHATZ_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8404644.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5069..5902
                     /locus_tag="ACHATZ_002131"
     CDS             5069..5902
                     /locus_tag="ACHATZ_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8404645.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5936..6775
                     /locus_tag="ACHATZ_002132"
     CDS             5936..6775
                     /locus_tag="ACHATZ_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8404646.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6801..7361
                     /locus_tag="ACHATZ_002133"
     CDS             6801..7361
                     /locus_tag="ACHATZ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8404647.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7560..9281
                     /locus_tag="ACHATZ_002134"
     CDS             7560..9281
                     /locus_tag="ACHATZ_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8404648.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9446..10588
                     /locus_tag="ACHATZ_002135"
     CDS             9446..10588
                     /locus_tag="ACHATZ_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8404649.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10604..11815
                     /gene="thiI"
                     /locus_tag="ACHATZ_002136"
     CDS             10604..11815
                     /gene="thiI"
                     /locus_tag="ACHATZ_002136"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8404650.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2528 g         3456 t
ORIGIN      
        1 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
       61 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
      121 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
      181 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
      241 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
      301 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
      361 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
      421 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
      481 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
      541 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
      601 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
      661 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
      721 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
      781 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
      841 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
      901 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
      961 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     1021 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     1081 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     1141 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     1201 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     1261 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     1321 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     1381 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     1441 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     1501 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     1561 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     1621 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     1681 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     1741 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     1801 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     1861 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     1921 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     1981 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     2041 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     2101 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     2161 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     2221 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     2281 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     2341 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     2401 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     2461 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     2521 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     2581 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     2641 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     2701 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     2761 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     2821 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     2881 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     2941 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     3001 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     3061 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     3121 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     3181 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     3241 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     3301 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     3361 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     3421 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     3481 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     3541 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     3601 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     3661 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     3721 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     3781 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     3841 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     3901 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     3961 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     4021 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     4081 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     4141 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     4201 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     4261 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     4321 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     4381 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     4441 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     4501 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     4561 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     4621 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     4681 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     4741 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     4801 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     4861 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     4921 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     4981 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     5041 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     5101 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     5161 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     5221 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     5281 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     5341 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     5401 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     5461 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     5521 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     5581 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     5641 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     5701 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     5761 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     5821 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     5881 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     5941 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     6001 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     6061 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     6121 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     6181 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     6241 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     6301 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     6361 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     6421 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     6481 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     6541 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     6601 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     6661 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     6721 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     6781 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     6841 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     6901 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     6961 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     7021 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     7081 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     7141 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     7201 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     7261 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     7321 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     7381 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     7441 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     7501 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     7561 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     7621 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     7681 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     7741 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     7801 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     7861 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     7921 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     7981 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     8041 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     8101 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     8161 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     8221 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
     8281 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
     8341 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
     8401 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
     8461 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
     8521 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
     8581 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
     8641 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
     8701 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
     8761 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
     8821 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
     8881 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
     8941 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
     9001 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
     9061 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
     9121 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
     9181 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
     9241 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
     9301 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
     9361 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
     9421 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
     9481 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
     9541 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
     9601 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
     9661 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
     9721 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
     9781 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
     9841 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
     9901 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
     9961 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    10021 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    10081 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    10141 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    10201 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    10261 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    10321 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    10381 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    10441 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    10501 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    10561 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    10621 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    10681 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    10741 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    10801 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    10861 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    10921 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    10981 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    11041 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    11101 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    11161 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    11221 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    11281 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    11341 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    11401 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    11461 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    11521 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    11581 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    11641 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    11701 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    11761 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    11821 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    11881 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    11941 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    12001 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    12061 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    12121 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYAJ010000095        12174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000095 DAYYAJ010000000
VERSION     DAYYAJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.095"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHATZ_002137"
     CDS             <1..610
                     /locus_tag="ACHATZ_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8404651.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHATZ_002138"
     CDS             723..1001
                     /locus_tag="ACHATZ_002138"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404652.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHATZ_002139"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHATZ_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8404653.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHATZ_002140"
     CDS             1458..2213
                     /locus_tag="ACHATZ_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8404654.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHATZ_002141"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHATZ_002141"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8404655.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHATZ_002142"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHATZ_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8404656.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHATZ_002143"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHATZ_002143"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8404657.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHATZ_002144"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHATZ_002144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8404658.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHATZ_002145"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHATZ_002145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8404659.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHATZ_002146"
     CDS             6950..7492
                     /locus_tag="ACHATZ_002146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8404660.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHATZ_002147"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHATZ_002147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8404661.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHATZ_002148"
     CDS             9085..9987
                     /locus_tag="ACHATZ_002148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8404662.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHATZ_002149"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHATZ_002149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8404663.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHATZ_002150"
     CDS             11430..11852
                     /locus_tag="ACHATZ_002150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8404664.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4046 a         2159 c         2687 g         3282 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tctt
//
LOCUS       DAYYAJ010000096        12162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000096 DAYYAJ010000000
VERSION     DAYYAJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.096"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..570)
                     /gene="frr"
                     /locus_tag="ACHATZ_002151"
     CDS             complement(13..570)
                     /gene="frr"
                     /locus_tag="ACHATZ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8404665.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(573..1295)
                     /gene="pyrH"
                     /locus_tag="ACHATZ_002152"
     CDS             complement(573..1295)
                     /gene="pyrH"
                     /locus_tag="ACHATZ_002152"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8404666.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1431..2312)
                     /gene="tsf"
                     /locus_tag="ACHATZ_002153"
     CDS             complement(1431..2312)
                     /gene="tsf"
                     /locus_tag="ACHATZ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8404667.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2411..3193)
                     /gene="rpsB"
                     /locus_tag="ACHATZ_002154"
     CDS             complement(2411..3193)
                     /gene="rpsB"
                     /locus_tag="ACHATZ_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8404668.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3552..4031
                     /locus_tag="ACHATZ_002155"
     CDS             3552..4031
                     /locus_tag="ACHATZ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8404669.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4248..5339
                     /gene="phnW"
                     /locus_tag="ACHATZ_002156"
                     /pseudo
     CDS             4248..5339
                     /gene="phnW"
                     /locus_tag="ACHATZ_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5332..6117
                     /gene="phnX"
                     /locus_tag="ACHATZ_002157"
     CDS             5332..6117
                     /gene="phnX"
                     /locus_tag="ACHATZ_002157"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8404670.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6463..8154)
                     /gene="argS"
                     /locus_tag="ACHATZ_002158"
     CDS             complement(6463..8154)
                     /gene="argS"
                     /locus_tag="ACHATZ_002158"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8404671.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8576..9523)
                     /gene="arcC"
                     /locus_tag="ACHATZ_002159"
     CDS             complement(8576..9523)
                     /gene="arcC"
                     /locus_tag="ACHATZ_002159"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8404672.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9638..10657)
                     /gene="argF"
                     /locus_tag="ACHATZ_002160"
     CDS             complement(9638..10657)
                     /gene="argF"
                     /locus_tag="ACHATZ_002160"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8404673.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10748..11977)
                     /gene="arcA"
                     /locus_tag="ACHATZ_002161"
     CDS             complement(10748..11977)
                     /gene="arcA"
                     /locus_tag="ACHATZ_002161"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8404674.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3331 a         2531 c         2181 g         4119 t
ORIGIN      
        1 tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac
       61 ttctttaatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc
      121 ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat
      181 gttacgaatc gcgatttttg cattttctgc ttcttttttc acatctttag ctaattcttt
      241 tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg
      301 actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc
      361 aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa
      421 tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg
      481 gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc
      541 ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata
      601 ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt
      661 ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg
      721 gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga
      781 tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt
      841 tctgccgctc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct
      901 gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc
      961 tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc
     1021 aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct
     1081 tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccaccc
     1141 acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct
     1201 ttgattactg gtggtttgat tccaaatcct gcatccccag ctaaagcttc tccactcaat
     1261 tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt
     1321 gccattttac catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt
     1381 aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc
     1441 acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact
     1501 tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc
     1561 atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc
     1621 aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt
     1681 tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca
     1741 taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt
     1801 gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat
     1861 gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata
     1921 acttgagttg cttcgatcaa gtcatattca attgtacctt tatctgtttt gattttcatt
     1981 gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa
     2041 tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct
     2101 gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc
     2161 ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct
     2221 tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt
     2281 tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt
     2341 ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca
     2401 tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga
     2461 ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt
     2521 ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat
     2581 cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag
     2641 ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt
     2701 caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt
     2761 caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag
     2821 ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat
     2881 cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag
     2941 ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc
     3001 cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt
     3061 tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg
     3121 gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag
     3181 aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta
     3241 ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg
     3301 atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat
     3361 acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag
     3421 tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga
     3481 cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa
     3541 ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat
     3601 tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca
     3661 cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa
     3721 atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg
     3781 aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact
     3841 ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg
     3901 agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct
     3961 cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat
     4021 cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata
     4081 ttttccttat taaataaacc tttacactct ttacaaaaat acatcatttt ttattcaatt
     4141 ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca
     4201 gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat
     4261 taactcctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt
     4321 gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc
     4381 ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc ggctcctttg
     4441 gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa
     4501 acggcgcata tggacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg
     4561 tttatgaggt atcgtaacat caaatcctcc aagctcatgt gattacacag ctgattgaag
     4621 agaatccctc tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga
     4681 ataatttgga agagattgct gctgtcacac aagaaaaaga tcttttgttt atagtggacg
     4741 cgatgtcaag ctttggtggg ataccgattc gtgtagccga actagggatt gattttttga
     4801 tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata
     4861 aagcggaact agaaaagcga aaaaatgtga gtcgaagttt gtcacttgat ctatatgacc
     4921 agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg
     4981 ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat
     5041 attcacgtta tctcgccaat aatcatgtac tacgacaaaa aatgacagag atggggttcc
     5101 agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact
     5161 ggcgatttga ttttaatcat ttttatcagt atttaaagca aaacggattt gtgatctatc
     5221 ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg
     5281 aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa
     5341 actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac
     5401 gcttttagaa atgcttttct agaaaaagga atccaactga ctgacaaaga aatccgcgaa
     5461 ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa
     5521 aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat
     5581 caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac
     5641 acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc cacgactgga
     5701 tttactgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaagg gtattcacca
     5761 gacttcatgg caacttctga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc
     5821 cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat
     5881 acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa
     5941 ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaaa
     6001 gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ctggcgctga tatttgtgta
     6061 aacaatttaa aagagttaac agaatacatt ttgtacgctg acaagaatcc agaatgactt
     6121 ttatacaaaa tcagaggtcg tgaaaaagct gtactgcatc aagtaataag aacagccaaa
     6181 caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
     6241 gaacaccgtt tattcgaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt
     6301 atttacaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg
     6361 actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct
     6421 ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc
     6481 atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc
     6541 taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt
     6601 gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc
     6661 agcagacaag accgtttcag ggtatttttg aactaattta accacttccc agctggtatc
     6721 gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct
     6781 gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac
     6841 ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac
     6901 tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc
     6961 gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat
     7021 ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg
     7081 gcgcatatcg tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa
     7141 atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt
     7201 acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt
     7261 aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc
     7321 gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa
     7381 agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc
     7441 tttcatcgat tcatcacgga accattgcca taattgaata gcctcttcat cgccttcttc
     7501 cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc
     7561 atggaattgg acatataaac gaagcaattc attgattggt tctgctttaa cagcttcttc
     7621 tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa
     7681 atggttgatg cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc
     7741 gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat
     7801 gtcgattggt actgttcctt gattcccgat attgctatcc ccataatgtt ctttttcttt
     7861 cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta
     7921 aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc
     7981 agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac
     8041 atctccgtgt tcagctgatt ttggattttc tagtaattgt tcgacttgtt ccagtgttaa
     8101 atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat
     8161 tcctcctaaa gtgctccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta
     8221 gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga
     8281 ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc
     8341 ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata
     8401 caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa
     8461 aaaaaaacga aaggctagtc gtttctgata aaaaaacaat ccagatggcc taaaggagaa
     8521 gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat
     8581 cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag
     8641 tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgcc tctactttcg
     8701 gaagcatgct tcctggagca aattgctttt catcaatgta ttgtttcatt tctgaaacag
     8761 taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc
     8821 ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat
     8881 ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc
     8941 caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg
     9001 cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat
     9061 cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt
     9121 agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa
     9181 cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca
     9241 tggcattttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag
     9301 gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt
     9361 gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat
     9421 actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga
     9481 ttgcattacc ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt
     9541 cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat
     9601 gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt
     9661 tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa
     9721 attgacgagc atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa
     9781 agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt
     9841 gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt
     9901 aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata
     9961 catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca
    10021 attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt
    10081 gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata
    10141 aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg
    10201 ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat
    10261 cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct accatttttt
    10321 gactgaatcc acggaattcg attccatcaa acatacgacc tagtacttta gctgtatctt
    10381 ctgtggattc ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac
    10441 ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata
    10501 aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt
    10561 tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg
    10621 ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg
    10681 tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac cgccgtcagg
    10741 aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac
    10801 ggcctcgtga caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca
    10861 attcgttcga tacataattt cggttataag tgactacaac acctggagcg attgccaatg
    10921 tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg
    10981 ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg
    11041 aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt
    11101 ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg
    11161 tatccaaatg catcattgca cggttgtttg gaattttgat tgctaacact ttttcaaatc
    11221 ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt
    11281 gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat
    11341 gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat
    11401 gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat
    11461 tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca
    11521 ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcgtataga ctgctagaac
    11581 gtacttcaat atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt
    11641 ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt
    11701 catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg
    11761 ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttt gcaaagttgt
    11821 catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa
    11881 tatctggtgt caaattttcc acttcttgtc caggcctttt cagtaaaact gtctttaatt
    11941 tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg
    12001 attacatgta taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa
    12061 tgagacattc ctcacattaa tattcgataa ttaaataatt aaacgttttt ctatttttaa
    12121 gcgtaattta ttcatttttt attctgtata cactttcaaa aa
//
LOCUS       DAYYAJ010000097        12087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000097 DAYYAJ010000000
VERSION     DAYYAJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.097"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACHATZ_002162"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACHATZ_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8404675.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACHATZ_002163"
     CDS             complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACHATZ_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8404676.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5535)
                     /locus_tag="ACHATZ_002164"
     CDS             complement(3220..5535)
                     /locus_tag="ACHATZ_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8404677.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="ACHATZ_002165"
     CDS             complement(5720..6580)
                     /locus_tag="ACHATZ_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8404678.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="ACHATZ_002166"
     CDS             complement(6639..7271)
                     /locus_tag="ACHATZ_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8404679.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7438..8199
                     /locus_tag="ACHATZ_002167"
     CDS             7438..8199
                     /locus_tag="ACHATZ_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8404680.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="ACHATZ_002168"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="ACHATZ_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8404681.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..>12087)
                     /gene="tuf"
                     /locus_tag="ACHATZ_002169"
     CDS             complement(11004..>12087)
                     /gene="tuf"
                     /locus_tag="ACHATZ_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8404682.1"
                     /translation="LGKKGLANPQDYASIDAAPEERERGITINTAHVEYETEKRHYAH
                     IDAPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNK
                     VDLVDDEELIDLVEMEVRELLSEYGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDT
                     VDEYIPTPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQ
                     KAVVTGVEMFRKTLDYGEAGDNVGVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVY
                     VLTKEEGGRHTPFFNNYRPQFYFRTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIA
                     VENGTTFSIREGGRTVGSGIVTEIEA"
BASE COUNT         3402 a         2570 c         2149 g         3966 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaat
//
LOCUS       DAYYAJ010000098        11997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000098 DAYYAJ010000000
VERSION     DAYYAJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.098"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..952
                     /locus_tag="ACHATZ_002170"
     CDS             56..952
                     /locus_tag="ACHATZ_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8404683.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1095..2036)
                     /locus_tag="ACHATZ_002171"
     CDS             complement(1095..2036)
                     /locus_tag="ACHATZ_002171"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8404684.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2162..3283
                     /locus_tag="ACHATZ_002172"
     CDS             2162..3283
                     /locus_tag="ACHATZ_002172"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8404685.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3541..5043
                     /gene="eat(A)"
                     /locus_tag="ACHATZ_002173"
     CDS             3541..5043
                     /gene="eat(A)"
                     /locus_tag="ACHATZ_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8404686.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5110..5637
                     /locus_tag="ACHATZ_002174"
     CDS             5110..5637
                     /locus_tag="ACHATZ_002174"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8404687.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5849..6910
                     /locus_tag="ACHATZ_002175"
     CDS             5849..6910
                     /locus_tag="ACHATZ_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8404688.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6913..7809
                     /gene="murQ"
                     /locus_tag="ACHATZ_002176"
     CDS             6913..7809
                     /gene="murQ"
                     /locus_tag="ACHATZ_002176"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8404689.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7823..9805
                     /locus_tag="ACHATZ_002177"
     CDS             7823..9805
                     /locus_tag="ACHATZ_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8404690.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9875..10321)
                     /gene="nrdI"
                     /locus_tag="ACHATZ_002178"
     CDS             complement(9875..10321)
                     /gene="nrdI"
                     /locus_tag="ACHATZ_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8404691.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            10532..11956
                     /locus_tag="ACHATZ_002179"
     CDS             10532..11956
                     /locus_tag="ACHATZ_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8404692.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         3809 a         2124 c         2644 g         3420 t
ORIGIN      
        1 actatttaaa caggctatca taggaaaaag tgacagagaa agtgaggcgg aacagatgaa
       61 caaaaacgac gtattgctaa ttgcgaaaaa taaatcactt tatttttttc cttccatccg
      121 ctctttagat gtttggtccg gaaaagattt actacagtcg gaattcatta aatcagctcg
      181 acaacagttg gcaaattatc agctgatcgt ttttttagat tatggatttg atatggagat
      241 ggctcgacgc gtgcgtccat ttaccaatgc aaaaatcgtg ttattttttt ggaaccactt
      301 taaagcagaa cacgaggcga tgctgaagca ggcacaagat gaaccggcaa tcgatgagat
      361 ttatcatttt gattttttag aagcgaaaga acttggacta aaacataata gttcctttta
      421 ttcgaaacat atggattttt cttctagcga accaaagacg gatctatttt ttggtgcaac
      481 aaataacggc agaaaagaac gggcagaaaa atataagcag gaatttacaa aaagaggtgt
      541 tacgaccaat tactttattc ttccgcttcg aggaaatgaa caacctggat accttactta
      601 tgaagaatat ctgaaaaaaa ccggggaatc gagggggatt ctagaactgt tgcgggaagg
      661 gcagcaagga gtgactttgc gaacgtttga atctatgtat tttcaaaaga aactagtgac
      721 agataatcaa gccatagctc attatcagtt ttatcattct gaaaatattt ttttgcttca
      781 agaacgtgat ttagatgaat taccggaatt tttgcatacg ccatttcaac caatcccatc
      841 agaactgttg gaattttttg atgcagaaaa ttgggcaaaa cgttttttca ctactgactc
      901 cgctatttat gaacaatatg aatacgatct ttcctctgtt aaaaataaat aaaagtgcca
      961 accaatcata gatgaaaata aacaagcgca attcttatgg caacatgaga attgtgtttt
     1021 tctttttgaa ctttcatggg ggtaaaaaaa tgagaaccag ctgctgatcc agctgattct
     1081 catcagagag ccttttattc tgcgccaagg attccttctt ttgcatggac taattgggtc
     1141 aaaaaggcac aatatggtgt agcaatatca tatttttgtc cttttcgcca aacggcacca
     1201 ttgatgtagt cgatttcagt caaacgatgg tttttgatca ggtcttggta catagatggg
     1261 taatgcaacc cgatgttgtc aggatcataa gtagaagcaa tatgttggta cacttcttct
     1321 tgatctaatg tgattccttc ttttgctgcg atagaagcaa actcagatac aatcgttcga
     1381 accatgccct ctgccgcttt ttgcgcacct aattcagcaa tattacaatc caatatcgtg
     1441 cataatccat ttaatgttcc gtttacacaa gcttttcgcc aaatggaata acgaacatta
     1501 tgactataga ttggattcaa ttttgcttct tgaaagactt ctacaacttt ttttgtaaat
     1561 gcttcgcctt ctggttccaa gttctctagt tcgatttctc catcacctaa taatgtgact
     1621 tttccgggac cagctagacc agctgtccac atcgtgatcc caaataagat atttttcttt
     1681 ggtacatatt tttccagtac atcttcatga cctaatccat tcagtaaaca caaaacataa
     1741 gtattctcag taataatgga ttggatcgaa cgaaacatat catctaattg attcgctttt
     1801 gtcaaggcta cgatcaaatc cacgtgtttg ttgctttcga tgatttcttc tggtgaatag
     1861 attggcagtt ttgcaactac ttctttgcca ttaaagtcag caatcaatcc atttgtacga
     1921 atggcttcaa tgtgagcagg ccagcgatcg atcaagacga cctcatttcc gctttgatgc
     1981 aacattaatc cgattcggct gcccattgct ccggctccag ctattgcgat tttcatgttt
     2041 tttctctcct ttattcaatg agttcccttt tagtatatag aaaaacagaa gaaaatagca
     2101 ttgttttgtt tgaaaaaagt gaaaaaaagt gtcttactta agaagaaagt gagggatata
     2161 gatggatatt ttggcggcaa tcgattcatt taaaggaagt gcttcttcat ttgaattaaa
     2221 tcaggcagtt ctaaatggtt gggaagaccg taaaggaaga aaaatcaatg tgccgattgc
     2281 agatggcgga gaaaatactg cagaagcaat ctattacgcg ctcgggggaa cctggcgtac
     2341 gattgatggg atggatctct tatttcgtca gcgaaaaatc agttatttat tgacgaactg
     2401 gcaaggaaaa aataccgcag tcattgaatc tgcacaagta ttaggattag atttacttga
     2461 taaaccaacg gatcaaacca tccgacttgc ttctagttat gccttgggag agcttatcaa
     2521 agatacactg cttcaagaag tggaacaaat cattgtcact ttaggaggaa gcggatgtag
     2581 cgatggtggt ctaggattac tccaatcttt gggtgcttct ttagaagggt acacagaagg
     2641 aaatcctttg ttatctacta gaaaacttca tttggatcga aggaacgttc ttgtttctaa
     2701 agtaaaagtg atcatcgctg cagacgtgac caacccatat acaggtcaac aaggagctgc
     2761 ttggcaattt ggaagacaaa aaggaggaac ggatacgacg ttagctttct tggataaaca
     2821 agctgaaaag attgctcagc agataaaaga tgtttgggag gtggatttag atgacatccc
     2881 aggaagcgga gcagcaggcg gtattggcgg agcccttttt ttgttaggag cgaagatggt
     2941 ctctggtttt gagttggtca gccaaatcac tggattagaa aaacagatcg aacaagcgga
     3001 tgtcatcatt acaggagaag ggcggattga tcagcaaaca atccacggaa aagttccata
     3061 tggcgtagca gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt gcggaagtag
     3121 agaccgagag ctggaaggac tctttgattt tctccccatt gtcttaagca ttcaattagg
     3181 accaatcgat ttagaggaag caatggaaca caggcaaaca ttagaaaaaa cagaaatctt
     3241 aggacagaca ctcagcagat ttttcaacat tctcccgaag tagttgacaa aaacaatttc
     3301 tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat tgaaaaatta
     3361 agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggga gcagtgtgca
     3421 gattttcggg attggcttgg ttctttcgat gtatgggtct ttttgtgtgc cccaaccctt
     3481 gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaata
     3541 atgtctaaaa tcgaaataaa aaatctgaca ttcggctatg acagccaagg cacattatta
     3601 tttgaacaag ccaatctaaa ctttgacaca caatggaaac taggacttat cggacgaaac
     3661 ggtcgaggaa agacaacttt actgaatatc ctgcaaaaca aactccctta tcaagggcaa
     3721 gtgatccatc agcaagcgtt tgtctatttc ccgcagcaaa caaaagataa agagcgtttg
     3781 acctattatg tgttaaatga gattatggat tttgagatat gggaaattga aagagagctc
     3841 caactgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact atcaggagga
     3901 gagaagacaa aagtcctact ggcactctta tttgtggatg acactcattt cccgttaatc
     3961 gatgaaccaa cgaatcattt ggatatctct ggcagaaaac aagtagcggc ttacttgaaa
     4021 aagaaaaaac aaggattcat cgtggtgagt catgatcggg gatttatcga tgaagtagtg
     4081 gaccatgttt tagcaatcga aaagagccaa ctggaacttt atcaagggaa tttctctatc
     4141 tatgaagaac agaaaaaact tcgtgatgaa tttgaaatag ctaaaaatga aaaattgaaa
     4201 aaagaagtca gcaggctaaa gaaaacagca gctgaaaaag cagaatggtc tcgttcccga
     4261 gaaggggaca aaacaaagaa aaaagtcgga ttcatcgata cggaatctag gcgagtgaat
     4321 aaaggagcag taggcgctga tgctgcacgg acgatgaaac gatctaaagc aatcgtgaat
     4381 cggatggaaa cccagatcag tgagaaagaa aaattattaa aagatatcga gtatatcgat
     4441 gctttgacga tgaatagcca agtgtctcat cataaacggc tgttaagcgt agaaaatctt
     4501 caattagggt atgaaaatct gttattcgag ccagttcatt ttacgatcga acctcatcag
     4561 cggatggcaa tttcaggtcc taacggcgcg ggaaagtcat ccattatcca ttatcttctg
     4621 gggacattca acggcaaggt tgtaggagaa aaatcccagc ccaaacattt gaccattagt
     4681 tacgtacgcc aaaattatga agacaatcga gggacattgg cggaattcgc gaagaaaaac
     4741 cgagtagact atcaagcatt tttgaataat ctccgaaagc ttgggatgga aagagatgtt
     4801 tttcataata aaatcgaaca gatgagtatg ggacaacgga aaaaagtgga attggctaaa
     4861 tctttatccc aaccagctga gctctatata tgggatgaac cattgaatta tttggatgtc
     4921 ttcaatcaag aacaattaga acaactgatt ttgaacgtga aacctgctat gttactagtg
     4981 gaacatgatc aaacctttct agataaagta tctactgaga ttatttctct tgagaaaatc
     5041 taaaccttgt aatctcaaag acaacaaggt atggttaagg agaactttgt caacgaagga
     5101 gagaatcaca tgctttatgt aacgagacac ggagaaacaa cttggaacgc acaaggatta
     5161 gtttgcgggc gagcagatgt gccgctgact gaaaaaggac agatgcaggc acaggcacta
     5221 gcagaaaagg tagtagattt gcctgttcca atcacgaaaa tcatccattc ccctcttcaa
     5281 cgagcaagag atacagcaca agcagtagtt gatcgtctca atttgccgat gaccgctgat
     5341 gagcgattgg tagaaatgga ttttggggat tatgacggac tgccaagcaa agatgaaaac
     5401 tttcaaaaag cgcgtttagc gtttgctgta cgtttcccaa atggagagtc ggtcttagat
     5461 gtgtacgcac ggatcgttcc tttattgaaa gaatgcatgg aagacgaaga aaatgtctac
     5521 ttgttagtct gtcataatgc cttgataaga gtgatcaatg cttattttca tccaatgcct
     5581 aatgaagggt tctttacatt catggtagac aatacagaat taatttctta tgagtaatta
     5641 gacttttgtg ttatttaaat aaaatgtttc aaggcactgc ttttgcttag cagtgccttt
     5701 tttgtggctg tgtcaattct tttggcatta taaatccaat ttttagaaat aaaatttaat
     5761 tattttaaca aaagtattga aattttgttt ctttagatat atactctagt catagaaatg
     5821 aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta tgaatgattc
     5881 tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat
     5941 tttcacttcc atgcatatac cggaagatga tgtttcggct tatcaaaatc gattgatcga
     6001 actaggaagc gaagcgaaaa agaataaact agagttgatg gtcgatattt caggagaggc
     6061 attgaaacga gctggctttt cattagaaga tctaacaccc ttgaaagcaa ttggtgtaac
     6121 agggttaaga atggattatc atatttctaa tcagcagatc gccgactggt cacatcaatt
     6181 gaaaatcagt ttgaatgcca gcacgatcac gcctaatgat atcgatgagt tgaaagaagc
     6241 agcggctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc ttgaaacagg
     6301 gttggacaaa gaatggtatc aggaaaaaaa tcaatggctc aaaactcaag gattccttgt
     6361 acaaggcttt gtaccaggag atacagagtt gagaggtcca ctgtatcaag gattaccaac
     6421 tttggaagaa catcgaggga tccatcctct ggcaggtgct cttgatctat tagcaagtaa
     6481 cacggatatt gtttatatcg gagatgcagg tctgtcagaa cacgttcagg agcaatttgc
     6541 ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagtttttta
     6601 cgaatatata ttaggaaaac atacgaatcg gcaagatgat gcaagagatg tcatacgaag
     6661 cgcagatgcg cgttttcgag agattccgcc aatcccagta agaaatacag ctaagcgtat
     6721 gaaaggatct attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt
     6781 aactaaatat gatttgccag cggatgaaaa agtgaatgta gtagccaaag tcatcaaaga
     6841 agaactaccg ctgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga
     6901 aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa
     6961 gaaacttttg gattagatga aatgagtatt tctcaagcag tccaactcat gaacaaagaa
     7021 gatcagaaag tggccgctgc tgttgaaaaa gaactgccaa gtatcgagcc agtgatcgcc
     7081 aacacgattg aatcctttaa aaaaggtggt cgcttgattt atatgggagc tggaacgagt
     7141 ggtagacttg gtgtattaga tgctgctgaa tgtgtaccga cattcggtgt agaaccggaa
     7201 atggtcatag gcctaattgc tggtggagaa gaagcaatga ctgtagccgt cgaaggagcc
     7261 gaagattctt atgaattagg gcaagaagat ctaatcaagc tgaatttaac aaaaaatgat
     7321 atagttattg gtatcgcagc tagcggtcgt acaccatatg tgattggtgg attagattac
     7381 gcaaaatcga ttggtgctgt tacaggaacg atttcttgca ataaacaagc agaaatcagc
     7441 gagcatgcgg atttcccaat cgaagtcgat gcaggaccag aatttttgac aggctctact
     7501 cgattgaagt caggaacagc acaaaaactg attttgaata tgatttctac tatcagtatg
     7561 atcggtatcg gtaaagtatt caataacttg atggtcgacg tcaaaccaac aaatgaaaaa
     7621 ctaattgaac gctcaaaacg aatcatcatg caagcgacag aagctgacta cgaaacagct
     7681 gaaaagtact ttgaggaagc agatcaaaat gtcaaactgg ctatcgtcat gattttgaca
     7741 aatagtgata aagaggcagc tcaagcaaaa ttagctgaag caaacggttt tgtaaaagga
     7801 accttatgag ggggaaaaga ccatggcaaa aaacgaattg actttggaag aactggcaaa
     7861 acaaatttat caaggagcag gcggtatggg caatgttgaa agcattgtcc actgcatgac
     7921 tcgtgtacgt atgaaggtca tcaatgatga tcttgtaaat gtctcggaat taaaagcgat
     7981 tccaggagta cttggcgtag tagaggatga acagctccaa gtcatcatcg gacctgggaa
     8041 agtgaacaaa gtagcgcagg aaatggttga cttagcaggc gtgaaattag gagaaaaact
     8101 tccttctgct agtgcaacta cagcaagttc ttctgcatct ggaaaagacc gtgtaaacga
     8161 acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa aaaccctcta aaatcaaatc
     8221 ggtattaaaa gacatctcta atatctttgt accaatgatc cctgcatttg tcggcactgg
     8281 gatcgtggca gggattgccg cagttttagc aaacttagtc acagctggaa cactagacgc
     8341 aggaacatgg caacaatata tcgatgtgat gaacatcttg aaaaacggga tgtttagcta
     8401 tctagttatc tatacaggta tcaatgctgc acaagttttt ggtgcaacgc caacgcttgg
     8461 tggagttatc ggggctgttg tgatgctgac agggatgaat ccagaagcac cactgaaaaa
     8521 cttgtttacc ggtgaagctc ttgcaactgg acaaggcggg atcttaggtg ttatcttcgc
     8581 agtttggtta ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg atgcggtaga
     8641 tatcatcgtt acgccaactc tttccctgat tgccatcggt ctgatcgaaa ttttcttcat
     8701 catgccttta gcagggttca tttctgacgg catggtcggt gggatcaact gggtacttgg
     8761 tgttggagga gcatttgccg gcttcgttct aggtacatta ttcttgccaa tggtcatgtt
     8821 cggactacat cagatcttga cgccaatcca tgtacaaatg attgatgaaa ctggtcgtac
     8881 cttattgctt ccaatccttg cgatggccgg agcaggacaa gtgggtgcag cattggcatt
     8941 atggatccgc tgcaaaaaag acaaagaatt aacagaaatg atcaaagggg ctttaccagt
     9001 cggaatctta ggaatcggcg aaccattgat ttacggagta acattacctc taggacgtcc
     9061 atttattact gcttgtatcg gtggaggtat cggtggggca gtgatcggaa tgatcggaaa
     9121 tgtcggagca atcgctattg gaccttcagg agccgctttg attccattga tctctgatgg
     9181 taaatggtta ggctatatcc ttggtttatt ggctgcttac gctggtggct tcgttgctac
     9241 attcttcttc ggtattccta aagaccgttt agaaaaagaa gaactagcga ccgaaacagc
     9301 cacagtaaat gaagtacctg aaaaagcagt acctcaagct tcagctgaaa gtgtgactat
     9361 cttatcatca gttgctgacg gaacagttgt ttctttagat aaagcaagtg acccagtttt
     9421 tgcacaaaaa atgatgggtg aaggttactt tgtagaacca gaaaacggac atatctattc
     9481 cccggttact ggagtaataa gttctgtttt cccaacaaaa catgctatcg gcattacgac
     9541 accaaatggc ttagaaatct tgcttcacat gggcatcaac acagttgact taggcggtac
     9601 gccattcgat gtgaaagtaa cagaaggaca gcaagtgaca cctgatacgc ttgtagcaga
     9661 agctgactta gaagcaatca aagcagcaga caaagaaacc tccatgatcg tcttggtaac
     9721 caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcga aagcaaaagc
     9781 acctgtgatg gatgtcaaag cttaataaga atagaaacaa tagagaacat aaaaagaaga
     9841 agccgaaatt tatttttcag cttcttcttt tgtgttattt atttttttct gccaaaattt
     9901 gataaacgcg tgtcacatca gatggtgttc cccgtaattc ataatctgcc aagaaaggaa
     9961 agccgaatct ttgtgcgtat tgtttggctg tcagacaata ttgattgttg aagtttttat
    10021 taccgctacc gactacacca aggcaaagtt tatgattgtc ttcgtattcc agatattcgc
    10081 gcatcgtttc ggtcaagatt tctgtgtcac cgttgtctaa cccattcccg ccgtctaaat
    10141 aagtaggaac gaaagtaaaa aatggttctg tttctttcgt aaaatcacta ttttcatgga
    10201 tttcttttaa agtaacttca ggattgacag gatcttctgc atgcattttt tcagcatatt
    10261 ctgctagatg tttggcaaaa gcacgcgtat ttcctgaaat cgatatatat aaaatgttca
    10321 tggatcatcc cccaattctt tccctttagt atagcatctt tttaagtttt tttggtggtg
    10381 taatttggaa agggactagg cgctttgctt gtataatgag aaaaaagtcg gtagaattat
    10441 atttctatta aagtttttat taattaagta aaaataaaaa aggtttgtta tactttttga
    10501 taaaagcggt tttactttgg aggatgctta catggataca gagcaacaga aaaaaggaat
    10561 tggtttgatt ccacttgctg ccttagtgat tggttcagcg attggaggtg gcgtatttgg
    10621 tattatgacg gacatctccg gatcggctgg tggcccagct atcttcagct gggtactcgt
    10681 aggaacagca atgttgatgc tctccttatc gatcaataat atcaataaaa aacgtccaga
    10741 tttaaaaggc gggatcttta gttacgcaga agaaggtttt ggccgtttcg ctggttttat
    10801 cagcggttgg ggttactggc tcacatgctg gttaggaaac gtggcgttcg caacattgtt
    10861 gatgagtgca ttaggttatt tcttccccgt attcgaaggt ggtcaaaatg tggcatcgat
    10921 cgtgatgagt acgatcttct tatggggata tgcatggcta gtgaatcgtg gagttgaaaa
    10981 tgctagcttt gtcaatgcta tcgtcacatt ttgtaaatta gtgccattat ttttatttat
    11041 tgtagtagct atcagcacct tttcgatcca acaattcatt aataactttt ctagcgatat
    11101 catattgaat gcgaatgggc agccgatccc gttttctagt caagtattga gttgcatcat
    11161 ggtgatgctc tgggtattcg ttggtattga aggagcttct gtcgtaggtt cacgagcaaa
    11221 aaaacaatcg gatgttggga aagcaacgat tctaggaata ttcggtttga tcattattta
    11281 cgtcttggtt tccatgcttc catatggcac aatgacgatg aatcagctgc aaacgatcaa
    11341 agcgcaacct gctatgggat atgtatttga aatgatggtt ggtaaatggg gggcggtcgt
    11401 gatcaacggc ggtttgatca tttctttagt tggggtttgg ctctcatgga caatcttgcc
    11461 gattgaaaca atggggaata tggctgagga cggacttttg cctaaagcat ggggaaaact
    11521 caacaaaaat ggggcaccaa cttatgctat cgtattgacg acgttatgta cgaatgtctt
    11581 tttactttca ttattattca ctgacagtgc atataacttt gcttatacac ttggtacagc
    11641 agcgattttc ttcacgtggc ttttcttagg gctgtaccaa atgaagcttt cttatcagcg
    11701 gaaagagtgg gtccaatttt tagtagggtt tttagcaagt gcttttcaag tatgggccat
    11761 gctagttgta gcctttaatg aagtgatgct tgttcttgtt cttttcttac caggtattta
    11821 tttttacctc aaagcacgta aagaacaaaa cgaacctgta caacatatcc acttagataa
    11881 gattttactt atcggtgtga cgatggtcgg agtaatcgca atggttttat ttgccactgg
    11941 gatgcttcac gtatagaagt ataaagagag gctgtgaagc ctgtggttta ttcaaaa
//
LOCUS       DAYYAJ010000099        11908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000099 DAYYAJ010000000
VERSION     DAYYAJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.099"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHATZ_002180"
     CDS             <1..465
                     /locus_tag="ACHATZ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8404693.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHATZ_002181"
     CDS             complement(1080..2120)
                     /locus_tag="ACHATZ_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8404694.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHATZ_002182"
     CDS             complement(2469..2795)
                     /locus_tag="ACHATZ_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404695.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHATZ_002183"
     CDS             complement(2782..3570)
                     /locus_tag="ACHATZ_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8404696.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHATZ_002184"
     CDS             complement(3938..4228)
                     /locus_tag="ACHATZ_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8404697.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHATZ_002185"
     CDS             complement(4364..4543)
                     /locus_tag="ACHATZ_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404698.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHATZ_002186"
     CDS             complement(4540..4890)
                     /locus_tag="ACHATZ_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404699.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHATZ_002187"
     CDS             complement(4883..6208)
                     /locus_tag="ACHATZ_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8404700.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHATZ_002188"
     CDS             6658..7272
                     /locus_tag="ACHATZ_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8404701.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHATZ_002189"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHATZ_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHATZ_002190"
     CDS             complement(7883..9202)
                     /locus_tag="ACHATZ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8404702.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHATZ_002191"
     CDS             complement(9336..9944)
                     /locus_tag="ACHATZ_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404703.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10582..10839)
                     /locus_tag="ACHATZ_002192"
     CDS             complement(10582..10839)
                     /locus_tag="ACHATZ_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8404704.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="ACHATZ_002193"
     CDS             complement(10832..11101)
                     /locus_tag="ACHATZ_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8404705.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="ACHATZ_002194"
     CDS             complement(11232..11411)
                     /locus_tag="ACHATZ_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404706.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11509..11745
                     /locus_tag="ACHATZ_002195"
     CDS             11509..11745
                     /locus_tag="ACHATZ_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404707.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DAYYAJ010000100        11755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000100 DAYYAJ010000000
VERSION     DAYYAJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.100"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHATZ_002196"
     CDS             <1..177
                     /locus_tag="ACHATZ_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404708.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHATZ_002197"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHATZ_002197"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8404709.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHATZ_002198"
     CDS             1199..1771
                     /locus_tag="ACHATZ_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8404710.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHATZ_002199"
     CDS             complement(2023..2280)
                     /locus_tag="ACHATZ_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404711.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHATZ_002200"
     CDS             complement(2406..3221)
                     /locus_tag="ACHATZ_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404712.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHATZ_002201"
     CDS             3738..5063
                     /locus_tag="ACHATZ_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8404713.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHATZ_002202"
     CDS             5056..5406
                     /locus_tag="ACHATZ_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404714.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="ACHATZ_002203"
     CDS             5388..5573
                     /locus_tag="ACHATZ_002203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404715.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHATZ_002204"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHATZ_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8404716.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHATZ_002205"
     CDS             complement(6724..7677)
                     /locus_tag="ACHATZ_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8404717.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACHATZ_002206"
     CDS             8289..9782
                     /locus_tag="ACHATZ_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8404718.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACHATZ_002207"
     CDS             9917..10213
                     /locus_tag="ACHATZ_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8404719.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACHATZ_002208"
     CDS             10314..11165
                     /locus_tag="ACHATZ_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404720.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACHATZ_002209"
     CDS             11186..11398
                     /locus_tag="ACHATZ_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404721.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4041 a         1811 c         2179 g         3724 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DAYYAJ010000101        11597 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000101 DAYYAJ010000000
VERSION     DAYYAJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.101"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHATZ_002210"
     CDS             6..305
                     /locus_tag="ACHATZ_002210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8404722.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHATZ_002211"
     CDS             378..1739
                     /locus_tag="ACHATZ_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8404723.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHATZ_002212"
     CDS             1761..2021
                     /locus_tag="ACHATZ_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404724.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHATZ_002213"
     CDS             2035..2787
                     /locus_tag="ACHATZ_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8404725.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHATZ_002214"
     CDS             complement(2881..3486)
                     /locus_tag="ACHATZ_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8404726.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHATZ_002215"
     CDS             complement(3539..4513)
                     /locus_tag="ACHATZ_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8404727.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHATZ_002216"
     CDS             complement(4891..5610)
                     /locus_tag="ACHATZ_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8404728.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHATZ_002217"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHATZ_002217"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8404729.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHATZ_002218"
     CDS             7161..8558
                     /locus_tag="ACHATZ_002218"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8404730.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHATZ_002219"
     CDS             8639..9079
                     /locus_tag="ACHATZ_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8404731.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHATZ_002220"
     CDS             9227..9553
                     /locus_tag="ACHATZ_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8404732.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHATZ_002221"
     CDS             9554..10303
                     /locus_tag="ACHATZ_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8404733.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHATZ_002222"
     CDS             complement(10383..10838)
                     /locus_tag="ACHATZ_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8404734.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHATZ_002223"
     CDS             complement(11265..11540)
                     /locus_tag="ACHATZ_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404735.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3659 a         2013 c         2356 g         3569 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatg
//
LOCUS       DAYYAJ010000102        11208 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000102 DAYYAJ010000000
VERSION     DAYYAJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.102"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            348..617
                     /gene="rpsN"
                     /locus_tag="ACHATZ_002224"
     CDS             348..617
                     /gene="rpsN"
                     /locus_tag="ACHATZ_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8404736.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            632..811
                     /gene="rpmF"
                     /locus_tag="ACHATZ_002225"
     CDS             632..811
                     /gene="rpmF"
                     /locus_tag="ACHATZ_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8404737.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            843..1220
                     /locus_tag="ACHATZ_002226"
     CDS             843..1220
                     /locus_tag="ACHATZ_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404738.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1241..1390
                     /gene="rpmG"
                     /locus_tag="ACHATZ_002227"
     CDS             1241..1390
                     /gene="rpmG"
                     /locus_tag="ACHATZ_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8404739.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1414..2397
                     /locus_tag="ACHATZ_002228"
     CDS             1414..2397
                     /locus_tag="ACHATZ_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8404740.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2427..2549
                     /locus_tag="ACHATZ_002229"
     CDS             2427..2549
                     /locus_tag="ACHATZ_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8404741.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2567..4090
                     /locus_tag="ACHATZ_002230"
     CDS             2567..4090
                     /locus_tag="ACHATZ_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8404742.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4114..4353
                     /locus_tag="ACHATZ_002231"
     CDS             4114..4353
                     /locus_tag="ACHATZ_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404743.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4546..5007)
                     /locus_tag="ACHATZ_002232"
     CDS             complement(4546..5007)
                     /locus_tag="ACHATZ_002232"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8404744.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5202..5420)
                     /locus_tag="ACHATZ_002233"
     CDS             complement(5202..5420)
                     /locus_tag="ACHATZ_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404745.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5538..7154
                     /locus_tag="ACHATZ_002234"
     CDS             5538..7154
                     /locus_tag="ACHATZ_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8404746.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7395..8735)
                     /locus_tag="ACHATZ_002235"
                     /pseudo
     CDS             complement(7395..8735)
                     /locus_tag="ACHATZ_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9173..9319)
                     /locus_tag="ACHATZ_002236"
     CDS             complement(9173..9319)
                     /locus_tag="ACHATZ_002236"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404747.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9358..9552)
                     /locus_tag="ACHATZ_002237"
     CDS             complement(9358..9552)
                     /locus_tag="ACHATZ_002237"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8404748.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9462..9794)
                     /locus_tag="ACHATZ_002238"
     CDS             complement(9462..9794)
                     /locus_tag="ACHATZ_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8404749.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10308..10499)
                     /locus_tag="ACHATZ_002239"
     CDS             complement(10308..10499)
                     /locus_tag="ACHATZ_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404750.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10593..10979)
                     /locus_tag="ACHATZ_002240"
     CDS             complement(10593..10979)
                     /locus_tag="ACHATZ_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8404751.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3724 a         1770 c         2164 g         3550 t
ORIGIN      
        1 attggcgata aaccagttaa acataaaaga ttgaataaaa agttatagtt tctttcatat
       61 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
      121 aaagtgaatt cttatttttc agagcctaaa ctgcatattc ttttaaatga tttgattatc
      181 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      241 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      301 ttacgatttg aggtgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      361 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtatgca gataaacgtc
      421 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      481 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      541 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      601 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      661 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      721 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      781 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
      841 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
      901 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
      961 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
     1021 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1081 acgaggagtt atttcaatgt atcgatggtt attgggagat acctgttgat atgaaagaaa
     1141 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1201 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1261 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1321 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa tttttaaaga
     1381 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1441 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1501 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1561 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1621 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1681 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1741 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1801 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     1861 gtcatggcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     1921 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     1981 agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca tcccttagtt
     2041 tttgaaagtg attcatcaga aaatcactat aatggcaaat actatcatca acaccatcaa
     2101 gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta
     2161 ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga
     2221 ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa cgaacaggtt
     2281 aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat cggaaaaatg
     2341 ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga
     2401 taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac
     2461 gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc aaagaacata
     2521 aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag
     2581 tgtaggactt attgggatag cagctctagg attattggga gcttgctcca gcactaatga
     2641 tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat
     2701 gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc
     2761 gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc
     2821 tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag
     2881 ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg
     2941 tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca
     3001 tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt
     3061 taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa
     3121 actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt
     3181 tgtgacgcaa cacgcagcct ttggatattt agctttggat tatggattga aacaagttcc
     3241 tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga
     3301 gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat
     3361 tgcaaaaact ttggccaacg aagctgggat aaaattggaa gtactgaatc ctcttgaaag
     3421 tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct
     3481 aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc
     3541 gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga
     3601 attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt
     3661 ggatgaagtt ttcgattaca aagcaaaaat caataaagac atgacagctg ccgagtacaa
     3721 agattattat actactggct ataagacaga tattgatact atcaacatta aagacaacac
     3781 cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa
     3841 aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc
     3901 aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac caagtaaggc
     3961 agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata atgaattgca
     4021 taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc aagaaatgat
     4081 ggctcattaa aattcaattt ttggaggaaa tacatggtaa gaaaagtatt attgtctatg
     4141 gattccaaga atattgatgc ttatcggcgt tatcgatttg caaagcaaat gagattaaat
     4201 gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga
     4261 gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt
     4321 ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta gatcattttt
     4381 cccttatttg gatttgatct gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca
     4441 ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat
     4501 tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt
     4561 ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc
     4621 atgaggatct ggaaaataaa agggcatatt attcaatgct tctgtaactg cgaaatgatt
     4681 ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct
     4741 taaaagttca atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc
     4801 tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac caccagtttt
     4861 gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg
     4921 acggccatgg atcgaatgag taaggcaaat ctttccacga gactcgacat gattttttgt
     4981 atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag
     5041 gggttctgta tcgaaaagat cattgcaaat tgcacgttag atcgtgataa aactaattga
     5101 aaagtccata tgttcatgct tcaaacgatt cgagatttcc tctggcgacc actgaaaatc
     5161 gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc
     5221 gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgaat agtttttttc
     5281 ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt
     5341 atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttc gagtatagta
     5401 agatgacgat actggctcat aattattttc cttctaggag tgttctcgac aaacttatac
     5461 tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt
     5521 atagaaagag gttccctatg aaaaaaatac ctattacaat cttaactggc tatcttggtt
     5581 caggaaaaac gatacttcta aatcatattc tgtacggtga tcacggaaag aaaattgctg
     5641 tcattgtaaa tgacataggg gaagtaaatg ttgatggggt attagtcgac aagaaaggtt
     5701 ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt tgtacattac
     5761 gcgaagattt gattgttgaa ctagatcaat tagtgcaatt ttctgacttg aatcaaattg
     5821 ttattgaagc ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt atattagcta
     5881 aaaacccttt agacattgat ttgacccaac gattagagat tggtgccatt attatagtag
     5941 tggatgtaaa acgtttttgg gataatttcg aaacgggtga aaagattcag gaaagaaaag
     6001 ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg cttgttaatt gatcaattcg
     6061 agtgttgtaa catattactt ttaaataata gtgacttagt gtcaaccaag acgatttaca
     6121 aaattaacgg tctaattgct aagttacagt ccaatgccaa aataattagg acaattaaag
     6181 gtgaagtaga cttatcgaaa attctaaata caaatctttt taatatagat gagacttcct
     6241 attcagtggg ctggttcaaa gagttagagc ttggctatca aaatcatatt cctgaaacag
     6301 aagaatatgg tatctcaagt tttatctatc gaaatcggtt actccttgac gcacaaaaat
     6361 ttgcccatct tacggataaa aattttcctg gcaatattac aagagcaaaa gggtttcttt
     6421 ggtttgccca acatagtgat ttttcgacga tctttgaaac atcgggtagc tataaaagta
     6481 ttgaatcatt aaattattgg gtagctagtt tacctaaaat tgagtgtcaa atgattatac
     6541 aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc attaataaac
     6601 tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt gagggttgtt
     6661 taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca tggaaaaagt
     6721 ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt gtcaagatag
     6781 gcgatgtcat aattattggc attttgatta ttacctcttt tattccctta tttgtattct
     6841 ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc gttcgaattg
     6901 atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc gtgaattatc
     6961 atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt cgagttaaag
     7021 aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa cccggtcaaa
     7081 cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa agcacagatt
     7141 actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa gatattatta
     7201 ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg
     7261 gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa cagtcggtta
     7321 gattacttat tttggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg
     7381 agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc
     7441 agttgggtag tattcttagt tttcttaaaa tattttcctc gatataaaaa gattcgttct
     7501 cggtagtttt gaaagtttct atagccatag gcaattcgtt ttagtacttt gatatggtta
     7561 ttcaaacatt ctatagggcc gttggaatag ggaacgcgca acgctcgttt gatttctgtt
     7621 tggtatttct tgaaggtcgt gaacactggt tggtagtgct ctggcaactc cttgtagtct
     7681 tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc
     7741 tggtagactt cgtagcctcg ctttaaggaa tcatcataag ataatagccg atcgactatc
     7801 tctgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg acctttcaat
     7861 tcccacgcat tcttttgaag tagtttccag tatttcttta agtgcttcga ccgacgatcc
     7921 ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaagcccg accaatgtgt
     7981 tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat
     8041 gtgcgatagg gtgtatagaa atctgaaacc actagacgca cttgttcacg gacttttata
     8101 gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc
     8161 agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac atttttcact
     8221 gatttgaatt catcgaaaca caccacttct ggtaacagct ggctcaaaga aatggtttta
     8281 ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt catacgtgcg
     8341 atcaaagaca tagaaagagg ttcgcttaat agctcaagaa tggcttgttt gactctgcga
     8401 gcaatggagc agcgacgatc actaactgaa tcttctgcta aaaaagtctt tccacagtgt
     8461 ttacatttga agcgctgttt gttaatccgc agaatggtct tgtagttact gacatcattc
     8521 aataagatgg atactttttt ccagccctat ttaatgatcg tttgttcatt ttgatagtgg
     8581 cgatgaaagc attcctccgg ttgataggtc aatgtgccat caaaaacaaa acatgtttcg
     8641 tcattgattc gctctttgcg aaaggcatct tcatgaaaga taagatttag gtctagaata
     8701 tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt
     8761 tcgtcgactt tattctaaag gattttaagg tgtctgtttg cgtttattag aaaaaagaaa
     8821 aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatccctt attttcttcg
     8881 cttcaaattg tcaaggagtc atcataaatt ttgtatggat tcttttgttt gggtagtagt
     8941 cttctaaata atcttggatt aacaccttca tctctatttt acttttatat ttgttggagt
     9001 cgattaattc tcatttgatt gatcgatgaa acgcttcaat tactgcattg tcaacaggag
     9061 tcccagtttg gctcactgaa tgtgtgatgt ggtggtcgtt caacagctct tgataaataa
     9121 aagaccgatg ctggctacct ctatcggaat gaattaccgt aggcaattct ccttaataaa
     9181 tttgtaatgg acggacgaca atctcttttt tcatgtgtga atctaccata tagtaacgta
     9241 gaatacgagt tgctaagtca atataggtgg acaaatagag gcgaccgtca ttacatggaa
     9301 tgtaagtaat atccatcacc caaacagcat caatttggtt ctgttcaaat tatcgattca
     9361 acattttttt accatttctt tgattgtttt ttctctacca tgagaagatt tgcgtcgatg
     9421 aaatcctctt gtgcataaat tcatttcacg cataagaaca gctacaacac gtttattgat
     9481 ttcgattcct tcatcgtaaa gtttttgagt gatccctgga gatccgtatc gatctttgtg
     9541 ttcgtagaac actcgtcttc ctcttttagt cgtttatttt cctgttcaag aatgtagaaa
     9601 tctgctttat caggtaatct ttttccttgt ccagcaaaag catttttccc gtatcgacga
     9661 tattctgaaa cccatcggga aattgttgga cgagagatat tgtattcttc agcaattttc
     9721 ttggtagtta cttttttctc aaggatatcc tgtgaaatct ttagtttaaa tttacgatca
     9781 tatgtttttc tcaatagagt tcattccttc cggtaacgta actgatagtc aagaaaagtg
     9841 tatcatagcc caacttagta tcagggtttg tcaactaaac tgtggaagtt gatagataag
     9901 agtttttaac aaccaaaatt ctcttgacta tttttgaaat cagacaaatt ttgattggac
     9961 acatagttga ataaatctac cgtcgttacg gaaggtaaat cgcatgcttt tcaattctag
    10021 aggagaagtc ctcgttgaag taccatagcc cagttctgga ttcgacctcc tgcccacttc
    10081 atacgtaaat aaagtaattt taggagtgag actgtgacaa aagttatttt taagctaaaa
    10141 acgaattaaa aaacgttgat aattaaaatc attcgtttta gattctatga atcattatag
    10201 aacgctgtaa aatcgatact ttataattat aaagaagata gtttcaaatt tattgttcga
    10261 aatttgaaac tatcttttta cttatgtcac agactccctt agttgagtta ttctttaatt
    10321 tcgaatttcg agatagctag atatattgca agcatacatt cttgataaca atcttgatca
    10381 tattttccag ttctccagga ttgcttatga atcaaagagc taaatctttt gagaattgcc
    10441 tcaacagctt cttcatttcc agcctttgct aaaatgaaat cttcttttaa agaattcata
    10501 aatctacctt cttcccatgg acttataatc tatttctttg ggaaggagga gaacgagcaa
    10561 agaagcatac aaaattagtt attatgcaga tattaaacca gcattttttg aatttttttg
    10621 taaagtcgat gttttaaatt tgtcgcaccc tgtcttgtaa tccccagaac ctttccaatt
    10681 tctttatcag ttttatcaaa gataaatttt tcgataagaa aaatttgttc aggttctttc
    10741 aacaaaggaa ggatttttgc taattttata tcttcaacaa ggagagtcac atttaaaaca
    10801 ttaatcggtt gtttaaattt tatagtatct ttagtaaaca aatatggtgg gagttgatca
    10861 agttgttcat gtttttgaat ttcttcttgt tttttataat aagtattagc ggtgttcaaa
    10921 atgacctttt caaagaatcg aatcaactgg gcatttacgc gttcgtcgtt atctgacata
    10981 ttgcacacct cctccattaa gatttaaagg gaaaagaggc aaaaaacacg aaaagaagct
    11041 aatgcaatta tgctgcgctg cgaataactt ctataagctc acatgcattg ataggactgc
    11101 tgatgtttat tttcaaaaat gtttttttag ttgataatgg atagttatct ttatacttag
    11161 aaaagaaaat tcatatatct tcttaaatat caaacatgtc cttatggc
//
LOCUS       DAYYAJ010000103        11150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000103 DAYYAJ010000000
VERSION     DAYYAJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.103"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..439
                     /locus_tag="ACHATZ_002241"
     CDS             <1..439
                     /locus_tag="ACHATZ_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404752.1"
                     /translation="LMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGN
                     DHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDA
                     VLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(500..865)
                     /locus_tag="ACHATZ_002242"
     CDS             complement(500..865)
                     /locus_tag="ACHATZ_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8404753.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(941..1186)
                     /locus_tag="ACHATZ_002243"
     CDS             complement(941..1186)
                     /locus_tag="ACHATZ_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8404754.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1256..1510)
                     /locus_tag="ACHATZ_002244"
     CDS             complement(1256..1510)
                     /locus_tag="ACHATZ_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404755.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1674..1997)
                     /locus_tag="ACHATZ_002245"
     CDS             complement(1674..1997)
                     /locus_tag="ACHATZ_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404756.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNVIVLFLYVLNYLDINP
                     LIFSR"
     gene            2184..2257
                     /locus_tag="ACHATZ_002246"
     tRNA            2184..2257
                     /locus_tag="ACHATZ_002246"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2218..2220,aa:Ile,seq:cat)
     gene            complement(2642..3139)
                     /locus_tag="ACHATZ_002247"
     CDS             complement(2642..3139)
                     /locus_tag="ACHATZ_002247"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8404757.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3179..3988)
                     /locus_tag="ACHATZ_002248"
     CDS             complement(3179..3988)
                     /locus_tag="ACHATZ_002248"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8404758.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4322..5179
                     /locus_tag="ACHATZ_002249"
     CDS             4322..5179
                     /locus_tag="ACHATZ_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8404759.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5172..6074
                     /locus_tag="ACHATZ_002250"
     CDS             5172..6074
                     /locus_tag="ACHATZ_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8404760.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6107..6457
                     /locus_tag="ACHATZ_002251"
     CDS             6107..6457
                     /locus_tag="ACHATZ_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8404761.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6506..7429
                     /locus_tag="ACHATZ_002252"
     CDS             6506..7429
                     /locus_tag="ACHATZ_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8404762.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7507..8400
                     /locus_tag="ACHATZ_002253"
     CDS             7507..8400
                     /locus_tag="ACHATZ_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8404763.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8438..9001
                     /gene="amaP"
                     /locus_tag="ACHATZ_002254"
     CDS             8438..9001
                     /gene="amaP"
                     /locus_tag="ACHATZ_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8404764.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9015..9203
                     /locus_tag="ACHATZ_002255"
     CDS             9015..9203
                     /locus_tag="ACHATZ_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8404765.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9216..10118
                     /locus_tag="ACHATZ_002256"
     CDS             9216..10118
                     /locus_tag="ACHATZ_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8404766.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10150..10392
                     /locus_tag="ACHATZ_002257"
     CDS             10150..10392
                     /locus_tag="ACHATZ_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8404767.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10648..11019)
                     /locus_tag="ACHATZ_002258"
     CDS             complement(10648..11019)
                     /locus_tag="ACHATZ_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404768.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3945 a         1918 c         2115 g         3172 t
ORIGIN      
        1 ccttatgata aaaaaactat ttttactatc aatattaacg ctagcaatgg caggttgttc
       61 tgactctggc ttagcaactg aactgatcac aaaatcaact gaagccacga cagaggagtt
      121 aaaatgtgga aacgatcaca gtgactacag aggcttaaat cgacgatgca tgggaatatt
      181 aggcagaagc aattgtaagc atcgatgtag taatcgaaga aggtgtagaa gaagcaacag
      241 aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga
      301 agatgcggta cttttaaacc acgtcaggag atgttcattt atgtctaggg aagttggcga
      361 tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag gatgaagata accctaaaac
      421 ttacgacatt ttagcttgat ctaagatggt taaagctttt taatgaagta agaggagata
      481 gaaggctttt ctagttatat taggtaagat aaattccttt ttgctccaaa tttgctaaac
      541 attctgctag ataaacttca tcgtgttccg gataaatcgg cacgttgcgc tctacagtag
      601 gtaaatcaaa aaatgcttcg catgatttgc cacgatacat aggatttttc cactctggtg
      661 ctgggctgta accacgtttt tccatttcat ccatgacaag ctgatgatat tggaataatc
      721 tataaggaga atagtgaaaa acataatcca cagtagcatg tttctttccc cagccatttc
      781 ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa
      841 tcaatttttc atgccataat cgcatagaaa tgtctccttt atatatagat tgttaacctt
      901 agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg
      961 tttctgagcg accaattagg taatcaacgg aaatatcgta aaagtcagcg atatagatta
     1021 attcaatgat gcttggaatt tcttctcctt cttcgtactt acgatatttg aataaaggaa
     1081 tacttaacgc gctagaaaca gctacttgag ataaagatcg gccagttctt aagaaattca
     1141 gacgacaacg taaagtatcc agtaaaatag cttcttcgat actcatttaa tcacctttca
     1201 ttatagtaga atagttataa tgactataga aagctacaca tgcaatcaat agtactcaat
     1261 gttttttatt taaacgtatc aaataaagaa tgacgatcaa taaagacaaa accagtcctg
     1321 ataattcttc tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa actttccaca
     1381 taaagtagat agccggaaga gtcaaaatag ctaaataaaa ccgttctttg ttccaattca
     1441 taagataaac aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa actctcacca
     1501 acgtaatcat gtaacggact cctcaacgat tcatataata aaagataaca tgttattgca
     1561 tacatcacaa atgaaatgct aggaaaataa aagaagaata gatcaatgaa aaatgaaaaa
     1621 gcagaacagt tttctgctga taagcaggaa ctgttccgct gaatgtgtct tatctatcga
     1681 gaaaaaatca atggattgat atctaagtaa ttcaatacat ataaaaagag gacgatcaca
     1741 ttcactaaaa aggaaagaat gatgatccca tagcgaatgg aaacatattt aacaaatact
     1801 tttgtttgaa tacgggcata tctttttcct ttatagctga gcatcatgac aagaagcaaa
     1861 agggtaatca caccttgaaa aatcattcca ataaacagaa ttcctgaggt ttgataattt
     1921 aattggagaa aataagctgc tgccgcaact aaatcgaata aaatcgcaat aaatggcaaa
     1981 ataatcactc ctaacattga gaataaataa aagaataaca ttttttctaa aataagcaaa
     2041 taaaaattaa agtaacaaaa tagttatgac acaagtaaaa gaaataagaa taacaatatt
     2101 attttactta tattttgaaa cttgtgagag tttgtcattc gtgctatagt tgaaaagtaa
     2161 caaaaataaa ttttatttat gatgtacctt tagctcagtt ggttagagca gacggctcat
     2221 aaccgtccgg tcgtaggttc gagtcctaca aggtacataa gaggttgtga aaaagctgcc
     2281 ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt acttggctgt
     2341 tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg tgaaatcagc
     2401 gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
     2461 ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt cgtgagcctg
     2521 ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc acgtaggact
     2581 aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa gatagatatt
     2641 tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg gattctccgt
     2701 gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga aaagcactct
     2761 gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact acttcgatct
     2821 ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa gtttcaggtg
     2881 gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa agaagaggga
     2941 tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt atatcccgtg
     3001 tgaagagaat atattcatta ttttggcgtg ctttagctaa tctttgagca atacgttctg
     3061 ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata tgattttgca
     3121 tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt tattcttttt
     3181 aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt gttacttggt
     3241 ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac ccgctgtaat
     3301 tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc gattcgattc
     3361 caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta ggcgtgattt
     3421 ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga tctctttgta
     3481 gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag ctaagagaag
     3541 gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg aaatgttcat
     3601 aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca tcttgggttt
     3661 ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct tttctcagtg
     3721 tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc ttcatttcag
     3781 caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg gaaaaaatat
     3841 ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca ttttcatcaa
     3901 atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca agcaaggtac
     3961 cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt acatatctat
     4021 tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat gaaactaact tgctttgtca
     4081 ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg ctatatatag tagtttttgg
     4141 tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt
     4201 gtgataaaaa aattttaaaa tattcaatga aaacaaagca tatattttgc tttaaaacca
     4261 aatgaggaat attctagatg tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt
     4321 gatggagaag agatacggcg tagttcctgt ggtaactgct tcagacgaaa attatgctcc
     4381 ttacttgagc gtgatgatcg caactgcatt agaaaattgc aataaaacaa gacgcataaa
     4441 attttatgtg attgacgatg gtctatcaga atatagtaaa gaaggattgg aagaaacggt
     4501 aaataaatat tcaagtaatg caagtatcca attcctgaca gtggaaaaag atatctacga
     4561 agacttccta gtcagtgacc atatcacaac taccgcttac ttaagaattt ctttaccaaa
     4621 cttattagct aaagaggatt ataagaaagt cctttactta gattcagatg tgttagtact
     4681 agacgatatc gtaaaactat atgatgagcc gttaaatgga aaaacaatcg gcgctattat
     4741 cgatccaggc caagtgaaag ctttggaaag attaggaatc gattccgatg acttgtactt
     4801 caattcagga gtgatggtga ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa
     4861 aactatccat tacctcagtg aaaatggcga tcgaatcatc tatcacgacc aagatgcttt
     4921 gaatgctgta ctttatgaag attgggaaca acttcatcca aaatggaaca tgcagacttc
     4981 gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg
     5041 aaatgaaaaa ccttcgatcg ttcactttac aggacatgat aaaccttgga atacgttgaa
     5101 agatcatccg tataccaatt tatacttaaa aaaattagca cacagtactc taacgaaagt
     5161 aggcgagatc aatgaataaa aaagaaatag cagtagtcgc aagttgtaac acaaaattcg
     5221 taccacattt agctgcatta tttgtctcag tattagataa ttgcaacccg tctaaatttg
     5281 ttcggttcta tgtgattgac gatgatatcg attttgaaag taaacagctt ttacgcttct
     5341 cagtgaaaaa cgcacgaatg aactcagatg tggaattctt gaaaatcaat aaagaattct
     5401 ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc ttattatcgt attgcgattc
     5461 cggaattgtt ccgcggcaca gaagttgaac gtatcttata tatggattgc gacatgatcg
     5521 ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg
     5581 ttgaagacgc tgggttccat caacgactgg aaaaaatgaa aatttcagct aaatcaatgc
     5641 gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa atggttagat gaaaatatta
     5701 cgcaaaaagt attagatttc atcgaacata atccagaaaa attacgattc catgaccaag
     5761 atgcattaaa tgctatctta catgaccgtt ggttaccact tcatccgcgt tggaatgcac
     5821 aaggctatat catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg
     5881 aagaaactcg caacaatcct tacattatcc atttttcagg acatgtgaaa ccgtggagca
     5941 aggattttga agggccaacg aagaaatatt acgaaaaata tgcagggatg acggctttcc
     6001 gttgtgtagc taaattccct aaatatccaa aatatgcaaa agttcaacca aaaacagatg
     6061 tagttgttaa ataatagaag accacaactg aagaggggga gtaggaatgg aaattacaat
     6121 gtctaaaaat gcagtagaga cgttaattga aaaggttggg gaaaatacaa aaatcgcact
     6181 cgcgcttatc aatgattccg atccatttct aagggacaaa ggtgcttttg caaaaggcag
     6241 cttttttcaa atcattcctt ttgtttcgga atttggagaa tatgcgacta aaatcgagca
     6301 tccattattg gacatctata catctaaatt agagcaaaat tatttcggta aacgattgaa
     6361 catggacttc aacaaacagc ttgacagctt ttcattagaa aatgaaatcg cagtactcga
     6421 ttataatatc aaattaaaga actgcttctt cagctaatag tttcgagaaa gtccaaaaag
     6481 gaggtataca ttgagatact actttgtgga tgaagaaaag aatgtaatag aaacagatga
     6541 aaaaaaatat aattggctcg tacttgatag tgcaaagccc gaagagattg agcaagcaat
     6601 cgaagaattc gagcttcctg atgatatttt cgtaggaacc gaatatccgg aagaagtttc
     6661 tcggatggag cgcctttatg agacgaaact agagcatcca ttttctttgg tagtgattaa
     6721 tctagatcag tcagataatc gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc
     6781 cgataatctc ttgattactt gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac
     6841 aaagcatgga aatcatttaa actcgtttga aaagatcatc acgtatacat tattagatat
     6901 ttacacccat tacgtgaaag aactaagaga gatgaaaaag cgaattgacg ctttggataa
     6961 agcagctcgt aaaacaacgg aaaatgaaga actgtataaa caggcagatc ttgaaagaga
     7021 catcgtatat atcgatcata ccttaagaga tcaacgggaa acaatggatc aattatggaa
     7081 ttctgaaact ttcatcaaac gtttagacga tgaacagcta ctctatgatg tccatttgag
     7141 acagcgacaa acagaaaaaa tgatcgagat ctatcgagat ttattagaga gtattggtga
     7201 tctattcaac gggatgatgg acaataatct aaatcatatc atgaaatatc tagattcagc
     7261 tgcattaatc atttccgttc cggcgttgtt cgcagggatt tggggaatga atacaggagg
     7321 attaccgggt aaaagttctg ctctaggctt tatactcgta gtctttggct ctttgatagc
     7381 agcagtagtt ttaggttacc atttataccg gaaagattat acaaattaaa gaatctattt
     7441 aaaacaggta tcaaacaaag aaatttgttt tggcaataca atttaaaaac taggaggaat
     7501 acagcaatga cagaaccaaa attaatcgac ccgcgaaact tgtaccatac ggagaaattt
     7561 cctaaacaag atcaggatac accagcttta cagcagaata tgattcctgt tcctgattgt
     7621 ggagaagaat cctatgaagg caataatcaa ctagaaaacc gccgtgtact aatcactggt
     7681 ggagattcag ggatcggtcg tgcagcagcg attgcatttg ctagagaagg ggcagatatc
     7741 gctcttcaat tcttcccagg agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa
     7801 gatgcaggac gtaagattgt cttactacct tttgatcttc gtgacgaaca agcacctaaa
     7861 gaaatcatag ataaagccgt gagtgaactt ggcgggctag atacgatggt actaaacgca
     7921 ggacagcaaa tttcttgtca gtctattgct gagttaccac ttgagcaagt caaagacaca
     7981 tttatgataa acatcatcgc tatgtttgct ttagtgaaag aagctgttcc gcatatccca
     8041 gcaggaggag caatcgtaac aacaacttct gttcaagcat tcaatccaag cgaacattta
     8101 ttagattacg cagcaacaaa agcttcaatc gctaacttta cgatcggttt agcaaaacag
     8161 cttgcaccta aaggaatccg tgtaaatggt gtagcacctg gtccaatttg gacaccatta
     8221 caactagacg aaggacaacc ttctgattcg atccctgaat ttggtcagca aagtttacta
     8281 gaacgtgcag gtcagccagg agaattagca ccagtttatg ttttcttagc atcgaataaa
     8341 gcaagttatg ttacagccca aatttacgga gtaactggtg gagaagcgat caacttataa
     8401 gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat
     8461 cggagtgatc ggaacattac ttttgctatc tgtgcttttg tttgcgatac tattaagtgc
     8521 aacctatcca ttgccttttc agctagagaa gtttcgattc ttttccatca ctaattcata
     8581 cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct attttgacaa ttatcacgtt
     8641 acttgttttc ctgttttatc caaaaacaat cggaaacttt ctactgaaac atggtgatgg
     8701 ggagttgacg ttggataaaa aagcaattga aggaatggtt cgttctcact tgcatgaaga
     8761 tgaattcatc cattcaccaa aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa
     8821 tgtaaaaggg gagttgaaac gaacttcagc actcattggt aaaacaggaa cattgatgaa
     8881 agaaatcgaa aatgaagtac aacacatatt gggaacaaaa gagccagtga aagtatcggt
     8941 gaaatacaca ggctttgaag aaccagctaa acgaagcaat gaccgcaaac gagtagaata
     9001 gggggggacc aaaaatgaaa gaactatttt cgacatacaa gctccctatc atctttgggg
     9061 tacttggatt gatattagcc gttttatgga tcaccgtggg ctttttcaag accttattga
     9121 tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta tctgaaacaa acgaaaatct
     9181 tagatcacta ttttaaatct taggaggaaa catccatgaa taacaataac aaaaataata
     9241 acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc agacccaacg acaccaaacc
     9301 aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc agtatctgca catccagatc
     9361 cagtagtccc taacgttcaa caaggagtag aagagaagaa acatgatgca gaaaagcatg
     9421 cacctcatca tccaggaact ccagaatcta aagttccaga acaacatgat gcaatcgatc
     9481 cttctaaacc tgtggaagag caaaaacatc cttctgacca tgcgaaagca caagaaaaac
     9541 ttagttctga ccacacaaac ggacaaggaa aacttggttc tgatcataca aataaccaag
     9601 gaaagcatga ttctgaacag gaagattcaa tgcgcggtga gctgaattat gaagataaag
     9661 ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga cggtttatta tctgcaaacg
     9721 gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac agataatgta acggctggga
     9781 tcgaaacaga agtcggtaaa aaacaagtag cagttgatat ggatgtcatt gtggaatatg
     9841 gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt aatcggcaaa gaagtaaaaa
     9901 atatgacaca tttagaagtg atcgaagtca acgctaacgt agtggacatc aaaacaaaag
     9961 aagaatttga aaaagatcaa gaaacggtgc aagacaaagt aactgacgca gcgaaaaaaa
    10021 ccggtgaatt tgcttcgaat caaacagata aagtaaaatc agcagctgga agcggcgcac
    10081 aaaaagtaaa agaaaataca gaaccacggg ttcagtaata aggaagtaaa acattaggag
    10141 gaacgaaaca tgggatttat ttggtcactt atcgtcggag gaattatcgg agcaattgca
    10201 ggagcaatca cgaataaagg ttcttcgatg gggattattg caaatgtcgt agcaggttta
    10261 gttggatcag cacttggtca agcattatta ggttcttggg gaccaagtat tgcaggaatg
    10321 gcaatcgtgc catcattgat tggtgccatc attttagtac taatcgtatc attctttgct
    10381 cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa agctgttctt catcaagtaa
    10441 taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttgggc atattatcga
    10501 agatgcaagc ttttgaacac tgtttatacg gaataagagg ctgtgacaag acttttgtca
    10561 cagcctctta tttatgcggg caaattccca attagatttt taaaagtttt tccttaaatg
    10621 tgtctttatt gatacagaga agcttcttta ataaaacaat gcctccctaa attttcttga
    10681 gataggaaag gtgaacatac aaaataatac gaccactttg aaaacccaga tgatccacaa
    10741 atggatcgta tgatactgat gaagtaaaaa gcaatggatc agaaactcaa agagattgaa
    10801 aaagacaagg aagaaataat atctcttttt tactcttgtc tgttttagcc aataatagct
    10861 cgtaaagtaa tctagacaaa caaatagaag taaacaaagt ttcgtaagag cagcaatcgt
    10921 atcatgttgc tctgctagac gattgacgag caggaaaaat gttggtaatg gaatatagat
    10981 cacgagtaat gaaagaaaaa agtaaacaat aaatggcata ctattcctcc taattgtgtg
    11041 agttcgccct cattttaaaa taacgatatt tgtttgtgaa gctttttcgt gtcgtaacaa
    11101 atgttatcta atgtattttt aaataaataa tcattattat caaaataatg
//
LOCUS       DAYYAJ010000104        10851 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000104 DAYYAJ010000000
VERSION     DAYYAJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.104"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHATZ_002259"
     tRNA            12..84
                     /locus_tag="ACHATZ_002259"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHATZ_002260"
     tRNA            88..160
                     /locus_tag="ACHATZ_002260"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHATZ_002261"
     tRNA            168..249
                     /locus_tag="ACHATZ_002261"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHATZ_002262"
     tRNA            267..339
                     /locus_tag="ACHATZ_002262"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHATZ_002263"
     tRNA            351..422
                     /locus_tag="ACHATZ_002263"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHATZ_002264"
     tRNA            439..524
                     /locus_tag="ACHATZ_002264"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHATZ_002265"
     tRNA            535..608
                     /locus_tag="ACHATZ_002265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHATZ_002266"
     tRNA            618..691
                     /locus_tag="ACHATZ_002266"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHATZ_002267"
     tRNA            711..783
                     /locus_tag="ACHATZ_002267"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHATZ_002268"
     tRNA            811..884
                     /locus_tag="ACHATZ_002268"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHATZ_002269"
     tRNA            898..971
                     /locus_tag="ACHATZ_002269"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHATZ_002270"
     tRNA            995..1084
                     /locus_tag="ACHATZ_002270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHATZ_002271"
     tRNA            1097..1170
                     /locus_tag="ACHATZ_002271"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHATZ_002272"
     tRNA            1189..1264
                     /locus_tag="ACHATZ_002272"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHATZ_002273"
     tRNA            1266..1336
                     /locus_tag="ACHATZ_002273"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHATZ_002274"
     tRNA            1350..1423
                     /locus_tag="ACHATZ_002274"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHATZ_002275"
     tRNA            1448..1535
                     /locus_tag="ACHATZ_002275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHATZ_002276"
     tRNA            1557..1628
                     /locus_tag="ACHATZ_002276"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHATZ_002277"
     tRNA            1651..1740
                     /locus_tag="ACHATZ_002277"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHATZ_002278"
     tRNA            1753..1826
                     /locus_tag="ACHATZ_002278"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHATZ_002279"
     tRNA            1845..1917
                     /locus_tag="ACHATZ_002279"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHATZ_002280"
     tRNA            1921..1993
                     /locus_tag="ACHATZ_002280"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHATZ_002281"
     tRNA            1998..2068
                     /locus_tag="ACHATZ_002281"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHATZ_002282"
     tRNA            2081..2154
                     /locus_tag="ACHATZ_002282"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHATZ_002283"
     tRNA            2166..2237
                     /locus_tag="ACHATZ_002283"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHATZ_002284"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHATZ_002284"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8404769.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHATZ_002285"
     CDS             3826..5298
                     /locus_tag="ACHATZ_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8404770.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHATZ_002286"
     CDS             5381..6169
                     /locus_tag="ACHATZ_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8404771.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHATZ_002287"
     CDS             complement(6236..6904)
                     /locus_tag="ACHATZ_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8404772.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHATZ_002288"
     CDS             7019..8731
                     /locus_tag="ACHATZ_002288"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404773.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACHATZ_002289"
     CDS             8734..10506
                     /locus_tag="ACHATZ_002289"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404774.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10851)
                     /locus_tag="ACHATZ_002290"
     CDS             complement(10588..>10851)
                     /locus_tag="ACHATZ_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8404775.1"
                     /translation="KNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTEL
                     GIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3111 a         1960 c         2501 g         3279 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt t
//
LOCUS       DAYYAJ010000105        10724 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000105 DAYYAJ010000000
VERSION     DAYYAJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.105"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACHATZ_002291"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACHATZ_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8404776.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACHATZ_002292"
     CDS             1995..4661
                     /locus_tag="ACHATZ_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8404777.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACHATZ_002293"
     CDS             5025..5777
                     /locus_tag="ACHATZ_002293"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8404778.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACHATZ_002294"
     CDS             complement(5799..6953)
                     /locus_tag="ACHATZ_002294"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8404779.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACHATZ_002295"
     CDS             7138..9579
                     /locus_tag="ACHATZ_002295"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8404780.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="ACHATZ_002296"
     CDS             complement(9909..10613)
                     /locus_tag="ACHATZ_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404781.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3420 a         1928 c         2263 g         3113 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcg
//
LOCUS       DAYYAJ010000106        10309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000106 DAYYAJ010000000
VERSION     DAYYAJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.106"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..289
                     /locus_tag="ACHATZ_002297"
     CDS             140..289
                     /locus_tag="ACHATZ_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8404782.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            300..1814
                     /gene="dltA"
                     /locus_tag="ACHATZ_002298"
     CDS             300..1814
                     /gene="dltA"
                     /locus_tag="ACHATZ_002298"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8404783.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1811..3016
                     /gene="dltB"
                     /locus_tag="ACHATZ_002299"
     CDS             1811..3016
                     /gene="dltB"
                     /locus_tag="ACHATZ_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8404784.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3061..3294
                     /gene="dltC"
                     /locus_tag="ACHATZ_002300"
     CDS             3061..3294
                     /gene="dltC"
                     /locus_tag="ACHATZ_002300"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8404785.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3297..4565
                     /gene="dltD"
                     /locus_tag="ACHATZ_002301"
     CDS             3297..4565
                     /gene="dltD"
                     /locus_tag="ACHATZ_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8404786.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4706..5788
                     /locus_tag="ACHATZ_002302"
     CDS             4706..5788
                     /locus_tag="ACHATZ_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8404787.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5788..6591
                     /locus_tag="ACHATZ_002303"
     CDS             5788..6591
                     /locus_tag="ACHATZ_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8404788.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6661..7401
                     /locus_tag="ACHATZ_002304"
     CDS             6661..7401
                     /locus_tag="ACHATZ_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8404789.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7412..8416
                     /locus_tag="ACHATZ_002305"
     CDS             7412..8416
                     /locus_tag="ACHATZ_002305"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8404790.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8461..10305)
                     /locus_tag="ACHATZ_002306"
     CDS             complement(8461..10305)
                     /locus_tag="ACHATZ_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404791.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3314 a         1703 c         2084 g         3208 t
ORIGIN      
        1 cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag taaaaaaacg
       61 gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt
      121 tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga attattgggg
      181 aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga tttatctgta
      241 tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg aggaagagta
      301 tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa cctgatcgag
      361 ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca tcagacgcac
      421 ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt tttggcaatt
      481 tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat gcctatttgc
      541 cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg gctaaaccat
      601 ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct gtcataacaa
      661 aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga aatagcgtaa
      721 aaggtacaga aaatttttat attattttta cttctggtac tactggagaa ccaaaaggcg
      781 tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa gactttggga
      841 tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta tcggtatttt
      901 ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa gcagttgttc
      961 aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg tgggtttcta
     1021 ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa aatgtgccgg
     1081 aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca gaaagtctac
     1141 ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa gcaactgtag
     1201 ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga ctaccgattg
     1261 gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag
     1321 gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac ctgaacaatc
     1381 ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac catacagggg
     1441 atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg gatcagcagg
     1501 tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg ctccaagaca
     1561 accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta cagcagctgg
     1621 ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg actaaatcta
     1681 tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag ttccattatg
     1741 tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta acagctgagg
     1801 tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt atttttttct
     1861 tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa aaagattacc
     1921 agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag gtaattattg
     1981 gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg tatggggcta
     2041 ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg ttattttagc
     2101 gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa aagcatcgtt
     2161 acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga tcatggaaat
     2221 aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc tgctgttctt
     2281 tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag atcagctcca
     2341 tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa atatttttgt
     2401 cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc ttcctgtagt
     2461 gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt atatgtacgt
     2521 gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag tcggtacaag
     2581 ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat catggaatat
     2641 caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg attatgtata
     2701 tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc
     2761 aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat tgacgtggta
     2821 ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg catggatccg
     2881 atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg gcttagccgt
     2941 atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat ttttagataa
     3001 actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga gaaaaggaaa
     3061 atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga agtaagaaaa
     3121 aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta
     3181 ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt cgagcgagag
     3241 gattggagca caccgaataa aatcattcaa caagcaactg cattgaaggg ttgagtatga
     3301 aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt
     3361 tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga cgtgcctctt
     3421 cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca ttatctgaaa
     3481 aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca tttcatccgt
     3541 ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga gcacccggga
     3601 cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg aaaaacaaga
     3661 aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa actgattact
     3721 ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg aagaaagtaa
     3781 cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta aaagagaatg
     3841 atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta cctgaatctt
     3901 tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta ttcagcaata
     3961 ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat
     4021 accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa acgacgaata
     4081 atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac gagccagagc
     4141 tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct gattttcagc
     4201 tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc ccaccggtta
     4261 acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga tttgccaaaa
     4321 agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt gtcaatcaag
     4381 cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc tggttggctg
     4441 cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt aagtatcatc
     4501 ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa ctacaattaa
     4561 aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat attctgaata
     4621 aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat aatcacaata
     4681 tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac ttattgattt
     4741 aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat tcagagaata
     4801 tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga ttgccaaaca
     4861 tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag ttggttttat
     4921 ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag gttggtgggg
     4981 gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg
     5041 tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata aaccttatga
     5101 aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca aagagtgggg
     5161 catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga atggcagtaa
     5221 atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc tgcgagtatt
     5281 agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg atgtgcaaga
     5341 agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg atattgcttt
     5401 tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag aagcagattc
     5461 gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc cgcataaaaa
     5521 attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc aatatacagt
     5581 gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg gtgttccgtc
     5641 tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga ttcatgaaga
     5701 tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg atagtgaaac
     5761 agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc tgaatatcgg
     5821 aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct tgttttctta
     5881 tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc ttttccggaa
     5941 gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt attcgcattc
     6001 aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc aaaaagtaac
     6061 cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat caatttgatc
     6121 aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt tttaatcagc
     6181 ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac gggtgtcatg
     6241 ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat ttttgtttca
     6301 gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac aaagttcgac
     6361 tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt gaaagataga
     6421 attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct aggagcaatc
     6481 gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt
     6541 tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta gcatgtaagc
     6601 agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg ggaatgtacg
     6661 atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca agggacaaca
     6721 ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag tgctacgctg
     6781 atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct tagccattat
     6841 catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact tcatcctaag
     6901 agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat tcactttgaa
     6961 gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga agaattaaaa
     7021 attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg ttacgccatg
     7081 cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc aggttatttg
     7141 gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac ctatcttttt
     7201 gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga acttgcgaaa
     7261 tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga tttagaacaa
     7321 ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact tgctgcagtt
     7381 gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg aaaaccacga
     7441 cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc
     7501 tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc aaaatacgct
     7561 tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc gtcgcttaag
     7621 tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta tcatgcctga
     7681 tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc aagcacttca
     7741 tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca ttgatggcat
     7801 gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc atggaacaat
     7861 tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa aagacaaaat
     7921 cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac catttcttcc
     7981 agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt
     8041 cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag attggaaaaa
     8101 aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg ggaagtctcc
     8161 agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag tccatttgaa
     8221 tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg gactaggaga
     8281 tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat ctctatcaaa
     8341 aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag aagaattgct
     8401 taccttactt tattgaaaat aaaaaatagc ggaatccaca tatatggatt ccgctatttt
     8461 ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc
     8521 tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca tcattaagtc
     8581 aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt ttgtatcgta
     8641 atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta tcgacaaata
     8701 aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat gctctaaagc
     8761 gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg gaagatctgc
     8821 ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg ctaagataag
     8881 atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg cttcttctaa
     8941 tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat cttgcagatt
     9001 ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat gctgtcgaag
     9061 ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta cttgtctccg
     9121 atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa cgattagaat
     9181 gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta atgccgtttg
     9241 gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac aagctacata
     9301 aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta aagtgcttat
     9361 aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag taaaagttgc
     9421 ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc cgtaccattt
     9481 tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg ttaagataat
     9541 aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac ttaaaatatc
     9601 ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa taagtgagta
     9661 ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc ctatagcgat
     9721 aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa taaaactaat
     9781 caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt tctgataagg
     9841 agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat ctatcacagc
     9901 caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata aaatcttttc
     9961 gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg cttcgtccaa
    10021 cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg taggaatcgt
    10081 acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga aaacccacga
    10141 atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat aaaagaaata
    10201 agcaattcct gttaaaaata acaatgataa tataatgatt atgctccact ttatacgtaa
    10261 ataaaaagat tcttttttat tatatatgtt attaaaaaaa gacataaag
//
LOCUS       DAYYAJ010000107         9991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000107 DAYYAJ010000000
VERSION     DAYYAJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.107"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..206)
                     /locus_tag="ACHATZ_002307"
     CDS             complement(9..206)
                     /locus_tag="ACHATZ_002307"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8404792.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(352..1230)
                     /locus_tag="ACHATZ_002308"
     CDS             complement(352..1230)
                     /locus_tag="ACHATZ_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8404793.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1269..2387)
                     /locus_tag="ACHATZ_002309"
     CDS             complement(1269..2387)
                     /locus_tag="ACHATZ_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8404794.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2384..4483)
                     /locus_tag="ACHATZ_002310"
     CDS             complement(2384..4483)
                     /locus_tag="ACHATZ_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8404795.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4534..5355)
                     /locus_tag="ACHATZ_002311"
     CDS             complement(4534..5355)
                     /locus_tag="ACHATZ_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8404796.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5352..6161)
                     /locus_tag="ACHATZ_002312"
     CDS             complement(5352..6161)
                     /locus_tag="ACHATZ_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8404797.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6182..6676)
                     /locus_tag="ACHATZ_002313"
     CDS             complement(6182..6676)
                     /locus_tag="ACHATZ_002313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8404798.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6688..7188)
                     /locus_tag="ACHATZ_002314"
     CDS             complement(6688..7188)
                     /locus_tag="ACHATZ_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8404799.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7253..9949)
                     /locus_tag="ACHATZ_002315"
     CDS             complement(7253..9949)
                     /locus_tag="ACHATZ_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8404800.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3033 a         2259 c         1647 g         3052 t
ORIGIN      
        1 aaaatctatc accaaaaaaa ttcaaacatt aagccaagga ttatgaagtc aacatttcga
       61 aaaatgaaca tacattggta agcaactata actattacca cagacataaa gtaacacaat
      121 atattactat ttaagtgtac gccaccagtg aatatcctca aggtatacag cgcacaacaa
      181 aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat ttaatgaaaa caacacgatt
      241 tgacttagag cctttttatt tgaattcatt ttcgctttat ccaacttcat ttgatcattt
      301 gattactcgg ttaaatactt ttgacatgag tatcaatgaa acattcatta attatggttg
      361 cttatttctt accgcttttc tggccaatgc taatccgccg attattccgg cattatcacc
      421 taattcgggc gtaataatgt agctatcaat tggtggtatt tcaacatatc catttataag
      481 ctcaacaaat ttatcctgaa cttttttctt catgtgttct tgtttcatta caccaccgcc
      541 aaaaataatt ctatctggcg aaaacatcag tgttgtattg tatgcacatt gggcaaggta
      601 aaaggcttca atctgccaaa acgaatcgtc agccaataaa ttctggcctt ttacacccaa
      661 ccgtttttca atcgctggtc ctgctgccat cccctctaaa caatcttggt ggaagggaca
      721 gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac atatgcccca tttccggatg
      781 actgaaccct tccacgaaag tcccattgaa caaggcacca cctccgatac ctgttcccac
      841 agtgaagtat gcaacacctg ctaacccttt gccagaacca gcaacatatt caccatagca
      901 agccgcattg acatctgttg tccattcaat cggaatattg aaatattgct ttagcgttcc
      961 tacaaaattg aaattttgcc aagcaagttt tggtgttgat gtgatatgac cgaaactcgt
     1021 tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc ccgatgcctg ccaattgttc
     1081 cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt tcaactggag ttgttgtggg
     1141 aaaactgact ctttccaaaa tcgttaattc ctcagtaccg actgcacaga caaatttagt
     1201 ccctccagct tcaattgccc catatatcat cataggttcc tcccttcgaa tatgtgaaaa
     1261 acacttcatt aacgcggcaa gcgttctcga tcagtgaaat gacgtttagt atttcctgaa
     1321 gcttctgttg aataccagta agcgacacta gcaacatcgt cttgccgctc aaataagcga
     1381 acatcatcgt tgccgatttg ttgtaaagtg accgataaat cttcggtaaa acagatgggg
     1441 tcttgaagat gccaacgata aagaccatgt cgtggcattg cgtcgtcacc aaaggcatga
     1501 acggcattca attttcccgt ttcaaaacga tctcttgtgg catctctatt tgattggtaa
     1561 ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg gacgtccgaa ctcatcgtac
     1621 tcatgaaagg cccacgcccc gccaaaataa tcttctgaac ctgttgaggt tacggtcgga
     1681 tactcactat ccccatcaat gtagaattta aactcgcctt cgccccacca ataacgttct
     1741 aggcaggtaa gcgcaaggta agtacccacg taatagcctt gaccctttaa tcgatcaatt
     1801 aacttataat cctcctgtaa tttggtaatg cgttcccgat gccaatttgc gtgaaaatat
     1861 aggctatttt cttccaattc gtccaccaaa gcgtaattta tcgtataaaa aaatgaggac
     1921 atatccttcg gatgtaaatt ctcgatcgta attttagcgg atgtttggaa aggcatttcg
     1981 aaataggaat taaagccccc tgtaggatta accactatcg gcaacgagtt cacctcgcaa
     2041 cgctctccaa acccattaca aaagaaatcc cctaaaggac tttcaacagc agggctatcc
     2101 gacccatccc aataaattct taagattaaa tcacgtaaaa caaaactgcc aaattctgtt
     2161 cgatcacgaa tcgtcatcca catatgacga attattcccg ttccagtaat atccgcaatc
     2221 acagtttctt caccagaagt cagcggaatt gcgccacgac ctttgcggct tggtcctagc
     2281 tcgcttggca acatcgcagc tttacctttc tcgcctgttg gattttcggg agtgattgct
     2341 cgactctgga tttttttaaa ttgggtaata ttttttagta tactcatctt tttctccttt
     2401 gaatgtgaat cgtttttccc ttttctacgt gaagaatttt tttcgtatca cttattttca
     2461 atggcttttc tgacaaattt gaaatactca actcggattg cgtacattca atcgaaatga
     2521 aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa attatcaaca ggatcaattt
     2581 gcaatactcc ttttttgaat ttcaatccga aatattgact gatcattagg ctgacaaaaa
     2641 gacccgatgc ccagccgcat ttgccgcagc caaagtctcg cacgacttct ccttgttgag
     2701 cctctaagcg atacggccac caccaccacg aaccgtctaa atctgccaaa caaattagtt
     2761 cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc gctcattaaa acagtgataa
     2821 agccaggaaa agtggcgccg gattctaatc cccaagtgat gcctttgatt tcctcgctat
     2881 aggttggatt gaaagaacta gcagaaaagg ccatggtgtt ctgatataaa tgatctttat
     2941 tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga ttcttctcca tcatgcatca
     3001 ataaattaat ttttccatcc acaatcacat cacttaaaaa gattaaccct tgctcaagaa
     3061 tcggtttttg atagtgcttc actgaatagg ttgttttctc ctcatcaccg attccctcca
     3121 aaaattgctc accaaaagga ccatcgatag tcatttgttt acaaattgat tcctttagtt
     3181 tacaggcaat gtttttgtat cgcttgctaa gactcagttg ttctagggct tcaaaaattt
     3241 ctgcaagtcc actacaggcc ttccaaagac aaagattgct gccggtatga tactttccaa
     3301 gtgcaaaggc gtctgataac caaacagatg gaaaaagcat tggttcccct gaccgacgtt
     3361 gagctaatat gtcctcaata attcttgaag cattcagtag aacctgcgga taccgctcaa
     3421 agaattttgt atccccagta tgttcaaaat aaagactagc cagtaacgca ctggataacg
     3481 aattactcag agaatgattg atacctcctg taaattttga accaggaaac ttaacgccat
     3541 attgatcaaa ccatagaacc gttttttgac ataactccgg ttcaaacatg gtgaatggca
     3601 atgatgcgta atacatatcc ttattccaaa ttcgattagt agcaggatag ctgccccaat
     3661 tgctgccgac gacctgtccc tgtcgattca taccgaaact agaaaagctt tgataaagtg
     3721 cacgacgata gagatggaca actctatcgt ttggcattga cagctgccca aatagctgag
     3781 caaaatagtt caatgtttcc ttcagccatt tttcgatatc agattcctga aaagtcatta
     3841 cttcttcaaa agcatttggg tccacaaaag caatggcgaa gcttgccact tcctgttgtg
     3901 cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt ttgctgatcc gaatacttct
     3961 cttggtacaa aggcacatct ttcactgaaa cttgaaggct ttcgtgtgtt tcattcacaa
     4021 tgtaaacctg ttgaacaagc atacttaaac gcttattagc cagtattgga gcaaaggata
     4081 ctacaatgac cttaaacgtc gaataatgat gaatatatct aggcaaacag tcttgaatca
     4141 catcgacttc aatacgatga tccgattctg acaaatggta gacaatcccg tttacttcaa
     4201 cctttaagga taacgaattg gtttgagaaa gctgtttatt gacccaaaca cctggttttt
     4261 cctcagttag taaattttct cgataaaagg tcaacgtttg aatcgtcccg caaacatcaa
     4321 ggtttcctaa tgtcttttga ttacttacat cgaaggtcaa tccctttaca tcaggaatat
     4381 tccgatagta gctgtattcg tttgaagtag tcacatttcc gtcgtttaac tcagcattaa
     4441 ataatgggtg tttgggtgaa taaattactt cattaatagt catcttttcc ctccttgcta
     4501 aaaaaactgc agcctagtag ctgcagcttc ctactatgcg attccaagta atgacaataa
     4561 aattcccaag acgaccattc caaaaataat tttgatgacg ccgacttttt tatctagtaa
     4621 tttgaaacag cccaatgtta ccaataacgg tacaattcct ttaaaaatac tatcgaggat
     4681 ttcttggaca ttcattagtt cttcgccctt cattgaaaag ctcaaaacgg tattgaattt
     4741 gaccatactg atcgtcattg cgcctaccat gatcagaccc aagatactag cagcttttgt
     4801 cagaatttca attaacccgt tcgcatacag cttttcaata tatttagagc ctaatgaata
     4861 gcctaaaacg ccgccatagt atcgcatcaa ataattaggt atattgaaca taactaaaaa
     4921 gataattggt gccaaaatat ttccggcatt ggctaagttg atcgctagtc ccgcggtaac
     4981 gacacgccaa actccccaga acaatgaatc gccaattcca gccaatggtc ccatcaaact
     5041 cgttttgatc gcgttgatac tttccacgtc aaaaccagct ttttcagaat tttccttctc
     5101 catcgatgcc gcaatcccca tgacaaagga cgcgcaagga atggtcgtcg aaaaatactg
     5161 tgtatgacgt tccagcgccg cagctcgatc ttcttcactt ttgtagaaat gattaatgaa
     5221 gggcatcata gaataggcga aaccaccgcc gcccatcgtc accggattca ccgaagcata
     5281 catagtcatc gagcgccaaa acatcttccg aaccaatttt ttctcatctt tagaaattcc
     5341 atcagctaat ttcattgaaa gaaatcctcc tcttcttggt caagcgttgc atttacagca
     5401 gcatcgcttt gaactgcatg aacagatata ttttttagtt gtagatctct ttgggctata
     5461 acaattgcaa taatcgttcc aactgcggca attgcaatca atggtaactc gagatacgcc
     5521 ataacaacgg ccccaaagaa aaaccacaca gcaatatttt tactccaaag catccgtaac
     5581 agcatagcca tcccaactgc cggcagcaga tttccagcga ctgttaatcc gttcatgatg
     5641 acctctggaa tattatccaa gacgcttttg acaacatctg ctccaaataa aacggcaaag
     5701 aatggaataa gagcataaag cgcatagtgg acaaaccaca taacgaagtg cagattgatg
     5761 attccttttt gatcaccaga ttttgcaaat ttgaaaaatt tagcagccat cggtccacat
     5821 acaaaagaaa aaatcatatt cattaactgt aatcctaata ctgctagtgg aaagacaatg
     5881 ggaataatcg catccacgtt tcctcccatt ttgattgcga acgctgtagc cagcccagca
     5941 gccaaagaag gctccgccgt actagagatt ccgatattga cgacacccat aaagatggct
     6001 tctagtgacg ccccgacaat caaccccgtc tgcaaatccc ccatcaacgc tcctgctaac
     6061 ggagcaacaa caatcggacg actcagcatg gacatcccga acatttcatg tccagcaact
     6121 ccaataaaaa cggccaaagc gactacaaat gcatcataca taatttttct ccttcctttt
     6181 tctaaaaatc actcaaaata gagatagctg aacgtttttc catcgccggt gtaggctgaa
     6241 aaacggtatc tggataaagt tcaactaatt cttttaagtc ttgaatttct gacgtcgtta
     6301 acatcacttc ctttgataag gttttcttat cttccgatgg cggtgtcatt ttcccagcat
     6361 tccccacgtt cattaattcg atttccgttg ggaaagcttt cgcaactttt accgcatctt
     6421 tgacattagg gactaaaacc atcaagcgca tgtccttcgc ccgcggatct tttaacaaat
     6481 gaatcgcgtc atcaactgtt ttaataatga ctttgacatt cgatggtgca gccatcttca
     6541 atgtcatctt ttgaatatcg ttggtagccg cctcatcgct tgctactact aacccggtta
     6601 aatttagact ctttgtccaa gtcatcgcta cttgtccatg aattaatcgt tcatcaattc
     6661 gcactgcttt aatcattttt cttcctccta aaaaaaatct tcctcttcat ttactacatg
     6721 tacctcatca agaatggtca tctttaattc ctgttggcat ttatttacca gttcctgaat
     6781 atcctccttt gttaattcct tggttgccaa tactatttcg agcagaattg gcagattaaa
     6841 gccagacaaa atagttacag caatctcttt ctccaaaaag gttttggtaa tcttctgatt
     6901 gacacttccg ccaaataaat cggtaaaaac aaaaatctga tcctgctcgt tcacctggtt
     6961 tacaaataca ttcaactcct gttcaaaatc acactcatcc acataagcat taatcaccgt
     7021 aatctcttga accttccccg tcagtaattg aatactgcta agaaagcctg aagctaagtg
     7081 accgtgagtc gctattagaa ttttcttcat atggttaatt cctttctttc tgttcacttt
     7141 tatataagca attcctgtgc caacttttaa aaacattggt ttaatcacgt taattcacat
     7201 taaaaaaagt gtctaaccta aaaaaaacag attagacact tttttagaca ctttattttt
     7261 gaatatacgg tcctaataat tcatagagca gcgaaagctc ataagcattg actttaaaat
     7321 tatatttcat ctccattgat tgaaaaatac ttctagaaac ataataaaat tcatcttcat
     7381 ctggtttcaa atcataattc ggttcttcag atttttctgt ccgagccaag aataaacgtt
     7441 ccatcatcaa tgccgtatgc atatagagat ttagttttac acggccattt aaagtgaaat
     7501 gatagtaatt ttcatattta gaaatgactg tttccacttc actcatcacg atcgttgggt
     7561 tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa gaattttact agctcctgaa
     7621 tcaagcggtt aaattctgcc ggcttcattt caggactcaa aatatgctcc aaattggctg
     7681 ctccatcacc gtctaagatg tcataaacat taatattagg aattgaaaag gaacgtggaa
     7741 gatccgaagt cgtgatgacc aattttgtct tgtcaaaata agtttcatca ttttggtcga
     7801 ttaattcacg tagttctctg tagtccttcg tgaaaacttc gatggattca tccgataaat
     7861 gatgacgaaa aacatctttc aatttgtccg aaatccccaa acccgacata caggaaatga
     7921 taatgttgct actttgagaa aagccttcaa agtattgaac attgattgaa tacttgtctg
     7981 ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt catcttcaag ccaatatcaa
     8041 gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc cccttttaaa tcgttcttga
     8101 tctgtgtgta aatttgattt aaagagccca tatctacgat cagcactaga ctatctgtat
     8161 tcacttgccg tttcacgaaa ttccttgtcg attcgacaat ctctgacaca ttactctcta
     8221 ctggcatatc caacgcttca aaaacataat tgccgcataa ttgattcact actgcttgaa
     8281 tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc cttcagctcg atggattcat
     8341 ctacataatc agataaagtc acgacaataa gcagtcttag aaagttctcg tccttttctg
     8401 agagcggtgg taaatgctcc aagaactgat tacataagta agtcgtccga ggtagatttg
     8461 cagctaatgt acgatttatc ccggttaagt ctgtcttata gcttggggaa atgacatagg
     8521 atctgctaat aatttctgca acaggtccac tcgtcgtcaa tccgtacgtt tttcctaaaa
     8581 cggataccca gacctttcta aacatctgaa tcatcaattt tctactcatt gataaaaaag
     8641 aatccttatc aatgtgatgc accaaatgct gtacttcatt ctttatcgaa gaaaaatttt
     8701 cattgaaatt accttcattt atcagctgag taattttttc caaaatcgaa tcataatcaa
     8761 acggtttgat tcgatccgtt tttatctttt gatttttctt tatcaataaa ctagcaatcg
     8821 gcaagctttc tacttcggat tccagcatat ttaactgaat caagatctca tttttcccaa
     8881 tcgttaatgg ttcttcttgt gttcgactat acacatccgc acaactgatt ttaatgatat
     8941 tcttcagctt accaatattc ccactaaagg gatgagataa cattaaagac aaggcttctc
     9001 ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt ttgaaaaaat aattgaatca
     9061 gctctaatcg ttctcgaatg gggcgatctt caaaagttgg caacataaca ctaatttgaa
     9121 tacgccgatc aaaggtgtct aataaataat tttcaccttt ttccgtcgtg gcgaaaataa
     9181 aacgaacctt agcagaatgc caatttacat tttcaccgac tggacgatag tttccagtat
     9241 caataaatag aaagagcttt tcttgatttt ctttcgatag gcgatggacc tcatcgagaa
     9301 aaagatagcc gttgttggcc gttgccaata aaccatcggt atccttttca gcccccgtaa
     9361 aggccccctt cttgtatcca aacagtgttg ccgaaagcag ttcgggattg tccgcataat
     9421 cggcacagtt taagatagca aatggtgcat ctttttcaat aatttgctca tggatagcgt
     9481 attcaaaaat ttttgtggct aaaaaacttt ttccaacacc cgtggctcca tttattaata
     9541 cactgagtcc gttaggcgga tatttgacag cggctataca tttttgaatg acctctctca
     9601 gacttccgtc atgaccaact acagaatgaa aggcttcgtt tatttgctcc gtattatcta
     9661 caaccgcata ttcatctaaa ggcttccaca aaacaggctt tcccggtaat ttctcgacct
     9721 ttttatcttc cactaatctc gtcaaataat ggctggcatt ctgacgagag atacctagat
     9781 tcttcgaaat tttctcggca gtgacagaag acatattccc tgttagtcgt atcagctcat
     9841 caaaaactcg atctttagcg ctctttttca ttttttcctc cagtttaatg tgtctaaaag
     9901 cataataaac tatcggctgc aatatgtcta ttctatattt gtattccaat ttttactttg
     9961 aaacacaaat agtcgaatat ttgcctcaga t
//
LOCUS       DAYYAJ010000108         9989 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000108 DAYYAJ010000000
VERSION     DAYYAJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.108"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..185)
                     /locus_tag="ACHATZ_002316"
     CDS             complement(42..185)
                     /locus_tag="ACHATZ_002316"
                     /inference="COORDINATES: protein motif:HMM:TIGR03949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HFM8404801.1"
                     /translation="MTLTKSYKELSSEDSKQITGGVIPLGVWLVGSFMAGYTVQSGYW
                     KRK"
     gene            complement(205..360)
                     /locus_tag="ACHATZ_002317"
     CDS             complement(205..360)
                     /locus_tag="ACHATZ_002317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8404802.1"
                     /translation="MKKYTELSVKETQNITGGGTFLNNFSAIKTTYDFGKWCADTVFG
                     AMGFKKR"
     gene            complement(822..992)
                     /locus_tag="ACHATZ_002318"
     CDS             complement(822..992)
                     /locus_tag="ACHATZ_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404803.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(1008..1544)
                     /locus_tag="ACHATZ_002319"
     CDS             complement(1008..1544)
                     /locus_tag="ACHATZ_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8404804.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQKLLDRLHEYEKENDKNIYISSKNVVVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKQKWWQLWK"
     gene            complement(1572..2309)
                     /locus_tag="ACHATZ_002320"
     CDS             complement(1572..2309)
                     /locus_tag="ACHATZ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8404805.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKNRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            2952..3038
                     /locus_tag="ACHATZ_002321"
     CDS             2952..3038
                     /locus_tag="ACHATZ_002321"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8404806.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
     gene            complement(3159..3848)
                     /locus_tag="ACHATZ_002322"
     CDS             complement(3159..3848)
                     /locus_tag="ACHATZ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404807.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKIEEFTA
                     CYEDMNDIFVKQEKRLTDKNMETFTRLYRKLPEKQQEEYQEKYEQVKKDMQYFKDTQT
                     EESCYDLFSDTIPFSTSEQERKERQQTVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4088..5002)
                     /locus_tag="ACHATZ_002323"
     CDS             complement(4088..5002)
                     /locus_tag="ACHATZ_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8404808.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHN
                     HIVINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            complement(4984..5364)
                     /locus_tag="ACHATZ_002324"
     CDS             complement(4984..5364)
                     /locus_tag="ACHATZ_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8404809.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(5652..5816)
                     /locus_tag="ACHATZ_002325"
     CDS             complement(5652..5816)
                     /locus_tag="ACHATZ_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404810.1"
                     /translation="MKLTKIVVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCCST"
     gene            complement(6307..7665)
                     /locus_tag="ACHATZ_002326"
     CDS             complement(6307..7665)
                     /locus_tag="ACHATZ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HFM8404811.1"
                     /translation="MKQDWENSSSVYSQKHQTFYRWILYPVIMFILLLGLFLTFAKKE
                     VVIRTSAKITANACKLEVPIDTKIIENQLVENKEVKKGEKLVTFDAQNLQLQKAPLET
                     ENEQISKEKESAQRLIDSLNTDSNQFQQPDPFGYEDQLKSILSENTTNQLEIEQKKLA
                     NQKEQEAYQKANNQLTKQLQSYKNQVTEWEQVRTAWQNQQSLQGFPTEIMTEYQAWQT
                     QVKEVSEEQKEQTKASITLQINERITQIKSMIEQLENEQVKLVEPTSIEPEIQKQTET
                     YIQIKEQAITATKQKIKELSSTENKNKQTIQLLNEQIQQAVIKAPITGKIHLNDEVKG
                     LTEIPKGTVIAEIYPALNKEKQLFTAYIPSSDMTRVKVNMPVHLKIDNKGVKTEILNG
                     KLTQIAETSTPTEKGDFYLVKGTISIPKKLDIRYGITGELSLIIGKKTYLQQIKDWIF
                     NE"
     gene            complement(7681..9825)
                     /locus_tag="ACHATZ_002327"
     CDS             complement(7681..9825)
                     /locus_tag="ACHATZ_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM8404812.1"
                     /translation="MRNYISQQDEKDCGVACLAMILKHYKTEIPLHKLRELSGTDLEG
                     TSAFGLKKTLEKLDFDCLAIQADSDLWEEKELPLPLIAHVIIDNKYPHYVVIYGIKDT
                     ELLIADPGRGKLKKSVIEFNEEWTGVVLIPTPKEIYQPTKEKIAGLNSFLPLIWKQKG
                     LVFHIILASTFITLFNIGSSYYFQGLLDYFIPNQAKSTLNIISIGLIAVYLFRILFEY
                     SRSYLLLILGQRMSISIMLSYFKHVLSLPMTFFATRKSGEILSRFLDANKIIDALASA
                     TLSVFLDIGMVLIVGVTLAIQNHILFFITLASIPFYLLTILAFVKSYEKANQEEMNAG
                     AILNSSMIESLKGIETIKAYNGEEQVYQKVDTEFIKLMKKSFKTVTLDNVQQGLKHGI
                     QLINSALILWAGSYYVMNGTISLGQLITYNALLVFFTDPLQNIINLQVKMQTAQVANK
                     RLNEIFAISPEQKNRTEKRKYSTSTFQQGISIENLSFSYNMKAPTLKNISCTILPNSK
                     IAWVGLSGSGKSTLAKLLVHFYEANEGSISYGKINGLDISHEELRNHVTYIPQESFFF
                     SGTIFENLIFGLTKIPSFEQILEVCDAVQLTEFINQQTLRFDTVLEEGATNLSGGQRQ
                     RLAIARALLKDSPILILDEATSGIDTLLEHKIMDHLLNLEDKTIIFIAHHLSIAKSCD
                     KIMVLHEGELVEEGTHESLRYNGGIYQKLWEV"
BASE COUNT         2849 a         1721 c         1581 g         3838 t
ORIGIN      
        1 aaaaaaaaca gccaagagag agtctctctc ttggctgttt tttatttacg tttccagtaa
       61 cccgattgta ctgtatatcc agccatgaaa cttcctacta accaaacacc aagaggaata
      121 acacctcctg tgatttgttt tgaatcttct gaggataatt ctttataact tttcgttaag
      181 gtcattactg agttctcctt ttaattatct ttttttgaag cccattgctc caaaaacagt
      241 atcagcacac cacttaccaa aatcataagt agttttgata gcagaaaaat tattcaaaaa
      301 agttccgcca ccagtaatat tttgtgtttc tttgactgat agttcagtat attttttcat
      361 tatttttctc ctttccacta ttttttgaga ttgtttgtgt tattaactaa taattgtttt
      421 agttaacaat aaatatatta aaatattttt atttaggaat ggtaatcgat gtcataaaaa
      481 gttgttttag attcataatc gtttaaatta aattataaaa tcgttatatt aagatctagt
      541 agacataatc gctactatgc caagtagccc ttgtatcaac cgatacaggg gctcactttg
      601 tgatttttaa ttggtctagg taaaaagctc tttttaaggc ttgattttct gcagttgctt
      661 ctttccgttt ctttgtgaat ttttaccagt cattttttac tgggttgtta tttttcataa
      721 aatatgataa atttttggta gcgcttcata agcgcgttgc tttttcaaga ataaacactc
      781 attatttttt aactggctgc tcgaaagagc agtctttttt attatttatc cgtttctttt
      841 acaaacaatt cttcgttagg actgttgtaa taatttggat tcaatttcaa ttcaccatgt
      901 ttattccctt tattgatcac tgacaataac atcggactga agcctagaat tcctagtaaa
      961 atagaaccga atagaagatt ttttcttgtc atgaatattc cctccagtta tttccaaagt
     1021 tgccaccatt tttgcttctt ctttgtgtgt ttttctatcg attctgtttc ttcagaaaga
     1081 atagtagttg attcatcttc tggagtttct tccgtataag ctaattgtaa ctgttcttgc
     1141 agtttttcga tctgttgatt tgcctgtagg gtgagctgtt gttgctggtc gagtaatttt
     1201 tgcatattag aattattttg ctctttaagg gaaagttgtt ctcgtaaaaa ttcattttct
     1261 tcacgcaact tatctagcaa ctcgtttaca accacgtttt tgcttgatat ataaatgttt
     1321 ttatcgtttt ctttttcata ctcatgcaac ctatctagta acttttggtt acctaagttg
     1381 ctagcaagtt gcaatactcc agaacgtaat acaaccttat aaccacgctc atttttagct
     1441 aataaggacg ggtctaattt atcaacatgt ttagagattg cttgcctact tatttcaaat
     1501 aattcagata attcctttat tgtaaaggtt tctttgggtt gcatgataat tgcctcctaa
     1561 attgttaggt attatttctc taaccaatta tctaaggtaa ttgaaggtat attttcttca
     1621 aaataaattt gcagagatcc tatcttattt cctttgtagg ctttaatttt tttgatttca
     1681 aaagttttaa aaatagcagg gtgagttgaa ttaacgttta aaaattcttt tttcgcttgg
     1741 gcaaaaattt gtttatcaat atctctcatt cgataacttt ctggtattgc taatagagct
     1801 ctaaaatctt ctatttttac aaaccagtga ccttttttag atcttctgtt tttatgagcc
     1861 ataagttggc gatataactc tttaacgtag ctactcttta agtttgtcat attttctaat
     1921 tcgaaacgag taaaattagt tccgattgaa ttaagtatat attcaaattc ttcagaaacc
     1981 tttattaata atgtttcatt cactttatcg attttaaatt tttgaaacag atttaattgc
     2041 tcatatgttt tttcgtcttc ataaataaaa gacaaagtaa tgattttttt tgacattttt
     2101 attaaatcat tagcaaaacg gtcgccacgt gcatttatat tgtaatcaat taattctttt
     2161 atttgtttaa atgtaaaaat ttcttctttt gtaccttttc ttttcatttt agaacatatt
     2221 ccccaaaata aatccatatc tataggagta aattttctta aagggacaga atttaatcca
     2281 ttattatatc gaactatatc gttactcaat aaaaatcacc tcaataaatt atgttatcat
     2341 agacataaga ggatgtaaaa tacaatttat cccccatagt gtgacaattt atcccccata
     2401 gtgtgacaat ttatccccca tagtgtgaca atttatcccc catagtgtga caatttatcc
     2461 cccactacct atgtcaatac ttactctccc atgaggttgc taatacctaa cagttattat
     2521 taacagtata tctaacagta tatctaacag tatatcttaa gagcacattt aatttcccgc
     2581 ctaaaaaact actacctttt actcaaacag gttcgcgaat ttttctaaac tggaaaactt
     2641 gctccctacg gtcgctaaaa aggtgtgatc gctatgctct cacattgtac tgcgcagtcg
     2701 cctaaaggct cctttgccaa aaccgttgtt caaagagaac cttgtggggc tctgagagct
     2761 catctaaacc gttttgagac agttctaagg gaaaacctta tcagttccta aagcgcctta
     2821 cagacggtca gggcaagccc taacaaccct aaaacaagca atacttaaaa acaccgcaca
     2881 aatttaattt tgtaaggtat aataacttgt cgcctatccg acagtcttgc gactggaagg
     2941 aggtgtatct aatgtttgaa cagttcattt gcccccttct tgtaggagtt tgtgtctctt
     3001 tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcattaaagg cgacatcaac
     3061 ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct aatgtttgat
     3121 catttgcaca ttcagtttaa catgatttta aatcaaattc aattttgctg ttgctgatct
     3181 aaccattcct tgacactttt taaataattt aatgccgttt cttttttagt cggattttgt
     3241 atgttagttg cttgctgaag tagggcttta taacgttcgt atgtgacagt ttgttgccgt
     3301 tcttttcgtt cttgttcact ggttgaaaaa gggattgtat cactgaataa gtcataacac
     3361 gattcttccg tctgcgtatc tttaaaatat tgcatgtctt tttttacttg ctcatatttt
     3421 tcctggtatt cttcttgctg tttttctggc aatttccgat aaaggcgagt gaatgtttcc
     3481 atatttttat ccgtcaatcg cttttcttgc ttcacaaaaa tatcattcat gtcttcataa
     3541 caagcggtaa attcttcaat tttttgtgct aatgcttgct cttttttctt tttatcacgt
     3601 gctgattcat acttttgagt gacatttcct aataaaatac ttctagaaac gcgcttatta
     3661 ccctctaaaa cgccgaataa gccgcttgaa acgattatca aggaaaatat acagccaagc
     3721 aagaaaaatc gcttaaatcg aattttagag ccttttaatg aatatcttgt ttgaaaaatt
     3781 ttatagatga gccaaatact tcctgaaata ctaacaaaaa tgagtagata gggtaaataa
     3841 aatgccattt tagcctcccc atactaacca aaatgcgaac agtgtcagga ctaagcaaca
     3901 taagggggca agaaaatcca ttgatcgttg aatgaacgcc tttttttgtt ctttatttcg
     3961 ctgatctaat agctcttgat ggagtgtttt aacttgtaaa tcaatcgtag tttgtatcct
     4021 ttcttgtgtg tagggagcaa gaaaagcacg ttgtttcttt atttcgtttt ctatgtcttt
     4081 taacgtttta gagcgataat tcttgttggt ggtctgttcc tctttcaacg tgttgttcat
     4141 ggtttcttcg agccggtcta atttccggtt gttcaggcgt aagagttcgt tgttctcttc
     4201 gtacttcttc tgcagtttcg cgaatgctag ctcgagctct tcgtagctcg ttggttcgct
     4261 gttggttata ggttcgttct cgtctgtcca gctcatgtaa aatcgtctcc ttttcgtagt
     4321 cctttcctag tctgttggat cgtacttttt tctttttgtg gatcaattcg taagtgaaat
     4381 ttttcccgcg attgaagcaa tgaatgccga atgttttgag ggaatcacaa aattggtcat
     4441 aggttctggt tttcttgttt ttcatggttt gatcaatcgc aaaccgaatt tcatctttcc
     4501 aaacaggttc atttttttct tttaatttga tttcagccat ggttcgtttt tcttcttttt
     4561 ttcctcgttc agtgatactt aaaccatgag ttaaacaaat ttcgtcgttg atatcgtaac
     4621 acttatctag gaatgcttga ccatgtgctt gaaacttttt tcctgtttct aaattgaccg
     4681 aattaatgac aatgtgattg tggatatgct ctttatctgt gtgcgtgtaa atcgcgattt
     4741 ggtattctgg cgcgattttt tcagcgtatt ctttccctag ttggttggct ttttcagcgg
     4801 taatttctcc tggtcgaaag gactgaatca ataaatgtgc ttgtacacga tcgtcttttc
     4861 cataaagcat gcgtatttgt ttcatttcac ttttggcata atcaatgtca cagtttattc
     4921 ctgttttgac ggttgctcgt ggttctgcgt aattgattgc tcggctacat gaagtggttc
     4981 gacttacttt aactgttgcc atatcttttg tagctccttt tgcatgattt ttaggttttc
     5041 ggataagcgt tgggcgtctc caggagcaac gtcgtgttgg tgtgtattac accacttagc
     5101 aatttgattg atattccctc cagctttggc taaatcgcgc gcgattgcct gggcaatgtc
     5161 tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttta cgaaagccgg
     5221 cacactcatt tgcaaagctt cagccgactg cttcagcttt gcaaattcgg attcgctcac
     5281 tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaat gatcgctagc
     5341 caaattttga ttttgttcgc tcataagtcc caccctttct gtacgtccta cggacgtatc
     5401 gttcgcaata tcattgtacc taaaaaggta caatgagtaa agctcactaa aagggtttgg
     5461 caagctctat atttgctagc caaataccta cgtatttggt gcaaataatc gcttgtcaaa
     5521 ggggcgcaag ctcccccttg aatccccctc aaaaaccacg ctccacaata caaaaaaggc
     5581 tagtaaatgc tagccacttt ttgtaaagtt tcgcttattt caccttcggt gaaattgttg
     5641 ttttacaacg attaggtaga gcaacatttt tttcgttgtt caaagattct tacgccattt
     5701 actattaaaa tcgcaggtat tccaaagtaa aggaagaatt gattagcagg caatacaaaa
     5761 tataaaataa tgccagcaat aacagctaaa attacaacaa tttttgttaa tttcataaag
     5821 cattcctcct attttctcct agcggatgta ataaacgccc taactgctga tccgaaaact
     5881 aatgatatca ttcctagtgt taattcggat agttccataa agcttagctg agacgttgtt
     5941 aagtattaat ttttttcact ttttatcctt cataaattat attatccaag taagaaagca
     6001 gataattatt atttgaataa ttatttctaa ccaataagaa taccgaatag agttgggtaa
     6061 aaaaacactt ttgttttcca tgttatcagt tatttggtat tgagaaggta atagtgtgtc
     6121 taacaaccag acagaaattg taagcaatag tgtgataagt aagaaattct ttattaattt
     6181 aaattcacag gcaattagaa ttatttgaac gattttaata tgtttgacca ttttttcttc
     6241 gtttttcatt tttttaatcc tctttatttt ttattaaata aacatatact ttcttgtcta
     6301 ctctactcat tcattaaaaa tccaatcttt tatctgctgt aaatatgttt ttttgccaat
     6361 gataagtgat aactctcccg ttatcccata tcgtatatca agttttttag gaatagaaat
     6421 tgttcctttg actaagtaaa aatcaccctt ttctgttggc gtacttgttt cggcaatctg
     6481 agttaatttc ccatttaata tttctgtttt tacgccttta ttatcaattt ttaagtgaac
     6541 aggcatattg acttttactc ttgtcatatc agaagaagga atataggcgg taaataattg
     6601 tttttcctta tttaaagcag gatagatttc cgcaatcact gtccctttag gaatttccgt
     6661 caatcctttt acttcgtcat ttaagtgaat ttttccagta ataggtgctt ttataacagc
     6721 ttgctgaatt tgttcgttta aaagttggat tgtttgttta tttttatttt ctgtagaaga
     6781 caactctttg attttttgtt ttgttgctgt aatcgcttgt tcttttattt gaatataagt
     6841 ttctgtttgt ttctgtattt caggttctat tgaggttggt tcaactaatt ttacttgttc
     6901 attttctaac tgttcgatca tacttttgat ttgtgtgatt cgttcattga tttgtaaggt
     6961 aattgatgct tttgtttgtt ctttttgctc ttcacttact tcttttactt gtgtttgcca
     7021 cgcttgatat tcggtcataa tttctgtagg aaatccttgt aaactttgtt ggttttgcca
     7081 agcagtacga acttgttccc attctgtcac ttgattttta taagactgta gttgttttgt
     7141 taactgattg tttgctttct gataagcctc ttgttctttt tgatttgcga gttttttttg
     7201 ttcgatttct aattgattag ttgtattttc agatagtatg ctttttaact ggtcttcata
     7261 tccaaatgga tcaggctgtt gaaattggtt cgaatcagta ttcaagctat caattaaccg
     7321 ttgtgctgat tctttttctt tcgagatttg ttcattttct gtttctaagg gtgctttttg
     7381 tagctgaaga ttttgagcgt caaatgtgac caatttttct ccttttttca cttctttatt
     7441 ttccactaat tgattctcaa taatttttgt atctattggt acttctaatt tacaggcatt
     7501 agctgttatt ttcgctgaag ttcgaatcac gacttctttt tttgcaaaag taagaaacag
     7561 tcctaaaaga agaatgaaca taattactgg atagagaatc catcgataaa atgtctggtg
     7621 tttttgacta taaacactag aagaattttc ccaatcttgt ttcattttat tttctccttg
     7681 ttagacttcc caaagttttt gataaattcc accgttatat cgaagtgatt catgtgttcc
     7741 ttcttccact aattctcctt catgtaaaac catgatctta tcgcaagatt ttgcaatcga
     7801 taaatggtgt gcaataaata taatcgtttt atcttctaaa tttaaaaggt gatccataat
     7861 cttatgttct aatagtgtat caattccact tgttgcctca tctaaaatga gaataggcga
     7921 atctttcaat aatgcacgag caattgctaa acgttgtcgt tgcccacctg acaaatttgt
     7981 tgcgccttct tctaacacgg tatcaaatct tagtgtttgt tgattgataa attcagttaa
     8041 ttgaacagcg tcacacactt ctaaaatttg ctcaaaactt ggtatttttg ttaatccaaa
     8101 gattagattt tcaaaaatag ttccactaaa gaaaaacgac tcttgaggaa tataggtaac
     8161 atgatttctt aattcttcat gtgagatatc taaaccgttg atttttccat agctaatact
     8221 tccttcgttc gcttcgtaaa aatggactaa tagtttagct aaagttgact taccagaacc
     8281 acttaaacct acccatgcaa ttttgctatt tggtaggata gtacaagata tatttttgag
     8341 tgtaggagcc ttcatattgt aagagaagga gagattttca attgatattc cttgttggaa
     8401 agtactagtt gaatattttc gtttttctgt acgattcttt tgttctggag aaatagcaaa
     8461 aatttcgttt aatcgcttgt ttgctacttg tgcagtctgc attttcactt gtaaattaat
     8521 aatgttttgt aaaggatccg taaaaaatac gagtaaggca ttatatgtga ttagctgacc
     8581 tagactaata gtgccattca taacataata agaacctgcc cataaaatta aagcactatt
     8641 tatcaactgt atgccatgtt ttaatccttg ttgaacattg tctaatgtca ctgttttaaa
     8701 agattttttc attaatttaa taaattctgt gtctactttt tgatacactt gttcttcgcc
     8761 attataagct tttattgttt caatcccctt taaactttca atcatactag aatttaatat
     8821 ggcgccagca ttcatttctt cttgattggc tttctcataa ctttttacaa atgctaggat
     8881 agtgagaagg taaaagggaa tagaagctaa agtaataaaa aatagtatat gattttgaat
     8941 agctaaagta actcctacaa ttagtaccat accaatatct aaaaaaacag atagtgtggc
     9001 gcttgctaaa gcgtcaatga ttttattcgc gtctaaaaat cgagaaagta tttctccaga
     9061 ctttctggtt gcgaaaaatg tcataggcaa actcagcaca tgtttaaaat aactcaacat
     9121 gattgaaata ctcatacgtt gtcctaaaat tagtaacaga taactccgac tatattcaaa
     9181 taatattcga aacaaataaa ctgcaattag tccaattgaa ataatattta aggttgactt
     9241 tgcttgattt ggaataaagt aatctaacaa gccttgaaaa tagtaggaac taccaatatt
     9301 aaataaagta ataaaagtag aagctaaaat aatgtgaaag actagtcctt tttgtttcca
     9361 aataagcgga agaaaagaat ttaagccagc tattttttct tttgttggtt gatagatttc
     9421 ttttggagta ggaattaaaa caactcctgt ccattcctca ttaaattcga taactgattt
     9481 ttttaatttt cctcttcctg gatcagctat caacaattct gtatctttaa taccataaat
     9541 cactacatag tgtggatatt tgttatcaat tattacatgc gcaattaatg gtaatggtaa
     9601 ttctttttct tcccataggt cactatctgc ttgaatggct aggcaatcaa aatctaattt
     9661 ttctaaagtt ttcttcaatc cgaaagcaga agttccttct aagtccgtgc cagataattc
     9721 tcttaattta tgtagaggaa tttctgtttt atagtgtttc aaaatcattg cgagacaagc
     9781 tacgccacaa tctttttcat cctgttgtga aatataattt ctcataaatt ctcctttaag
     9841 atataattat ttaaaaaata gaaagggaag taatatgaaa aaaaatgata tttggttaaa
     9901 agttggaaaa gtactcttaa taataattgt ttttatatta gggtcgttat ctgctaaaat
     9961 gatattttct ttatttttag aacataggt
//
LOCUS       DAYYAJ010000109         9787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000109 DAYYAJ010000000
VERSION     DAYYAJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.109"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..1779
                     /locus_tag="ACHATZ_002328"
     CDS             16..1779
                     /locus_tag="ACHATZ_002328"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8404813.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            1863..2321
                     /locus_tag="ACHATZ_002329"
     CDS             1863..2321
                     /locus_tag="ACHATZ_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8404814.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2430..3113
                     /locus_tag="ACHATZ_002330"
     CDS             2430..3113
                     /locus_tag="ACHATZ_002330"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8404815.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3260..3874
                     /locus_tag="ACHATZ_002331"
     CDS             3260..3874
                     /locus_tag="ACHATZ_002331"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8404816.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            3890..4096
                     /locus_tag="ACHATZ_002332"
     CDS             3890..4096
                     /locus_tag="ACHATZ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404817.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      4321..4487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            4635..5192
                     /locus_tag="ACHATZ_002333"
                     /pseudo
     CDS             4635..5192
                     /locus_tag="ACHATZ_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            5250..7289
                     /locus_tag="ACHATZ_002334"
     CDS             5250..7289
                     /locus_tag="ACHATZ_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8404818.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(7398..8549)
                     /locus_tag="ACHATZ_002335"
     CDS             complement(7398..8549)
                     /locus_tag="ACHATZ_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8404819.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(8703..9233)
                     /locus_tag="ACHATZ_002336"
     CDS             complement(8703..9233)
                     /locus_tag="ACHATZ_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8404820.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(9332..9682)
                     /locus_tag="ACHATZ_002337"
     CDS             complement(9332..9682)
                     /locus_tag="ACHATZ_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8404821.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         3522 a         1708 c         1923 g         2634 t
ORIGIN      
        1 aggagagaat gtattatgag tacagtatta catgtattgg atgcaatcaa tagccccgca
       61 gatgtcaaaa aattatctgt tgaggaaatg aaacagttgg cagaagagat gcgccaaaaa
      121 atcttgcaaa aggacagcga tgtaggtggg cacgttggtc cgaatctagg tgtagtagaa
      181 ttaacgattg cgtttcatta tgtatttgat tcaccaaagg ataaaatcgt ttgggatgtt
      241 tcccatcagt cttatggtca taagctttta actggtcgaa aaaatggttt tgaaaaagga
      301 cattaccgtg aagtgagcgg atattccaac caacatgaaa gtaaacatga ctattttaca
      361 gtggggcaca catctacatc tatcagctta gcaacaggtt tagctaaagc gagagatctg
      421 aagaaagaat caggaaatgt ggtagcattt atcggtgacg gctccctaag tggtggctta
      481 gcttttgaag gattgaacaa tggtggtgtt ttacaatcca atctaatcgt attagtaaac
      541 gataatgaga tgtctattga tgagaatcat ggcagtttat ataaaaactt agctgagctt
      601 cgtcaaacca acggtaaaag cagcaataac ctatttaaag caatgggatt tgattatcgt
      661 tacgtggata atggaaacga tctggaaacg gtgatccgtt tatttgaaga agtaaaagat
      721 attgatcatc cgattgtact tcacgtccat acggaaaaag gtcgcggtta tcagccggca
      781 atcgataata aaatgaaata tcattggcat gtaccatttg atctgtccac cggggaggca
      841 aagcaagtaa cttctggaga aacatttaat atgatcatca tggattattt ggataagaaa
      901 atgcaaacaa gtacagctcc tattgccgca gtcaatgcag cgataccagg cgcctttgga
      961 ctgaaaacat tccaagaaaa acatccagat cattattttg acgtggggat cgctgaacag
     1021 cactctatca ctttcgcttc tgctttggct tctcagggag tacgtccagt cgtcttccaa
     1081 tcaagtactt tcttgcaacg ggcatatgat caattatcac atgatttagc gatcaacgag
     1141 aatccagtga ttattttagt cggtggcgga acgatctcag ctagtgaccg aacacaccaa
     1201 ggaacatttg acattcctta cttatcaagt atcccgaaca tcaaatatct tgctccagca
     1261 acaaaagagg atatgatctc tatgctagat tgggcgttcg aacaaacagc gcagcctgta
     1321 gcaatccgaa ttcctacaca tggtgtagaa catggtacag ctatcgcaaa tgattatagt
     1381 attccagcct atcaaacagt aaataatgga ggaaaagtag caattttagc tttaggcggt
     1441 tttcttgaat taggaaaaga aacagtaaaa gcttttagaa aaaaaggaat cgaagcaacg
     1501 ttgatcaatc caggaacgat cacagaaaat gacgtagaaa cattagataa attaaaagaa
     1561 acacacgaat tagtcatcac attagaagat ggtagtttgt caggaggatt tggcgaaaat
     1621 atcagtcgtt attatggtga ttcttcaatg aacgtattaa attttggtgc agagaaagaa
     1681 ttcacggata gcgttcctgt agggcagctt tatgaacgtt atcatttaac accaaaacaa
     1741 atcgttgaag atagtctgcg tgtcttgaat attcaataat tcattatgct caccatccag
     1801 ataattgttt atactagtat ctggatggtt ttttcattat aaaaagcaaa ggggtggatg
     1861 ttatgtatca gcaagtagaa gaatatgtca aaaagttcca agaaaaatat cctagttatc
     1921 aatcagaaaa attaggtttc ttttcatttg gggatacaga aaaaatggca gacgagttga
     1981 gcgaactagt tgtaaaaaag ataaagactg gtacatcaag tggttacgat ctgtataaac
     2041 aagagaatga gcctttacca aaagttggtc aattagacgt ggtcctgaat ggaaaaaatg
     2101 aaccagtttg tatcatcaaa aatataaatg tgaccatctt gccttttagt gaagtcacga
     2161 aagagcatgc ttggaaagaa ggagaagggg atcggacatt agcttattgg cggaaagtcc
     2221 atgctgagtt ttttaaacat gcttataaag aagcgttgaa catagaattt acagaagaga
     2281 agctggtcgt atacgaagaa tttgaagtaa ttttctttta gtaagaaagg tctgctgaaa
     2341 gaaatagctc attattgtct cctgaataga aaagggttac atttttatat gaagctgtat
     2401 ttttatataa cattactgga ggagatattg tggatacact ggaacaaatc aaaggcggag
     2461 tcgtcgtttc ttgtcaagct ttggaaaacg agccgctaca cagttctttt attatgggga
     2521 gaatggcttt ggctgcaaaa gaaggcggag cagttggtat tcgagcaaat acgacagaag
     2581 atatcaatga aatcaagcga atcgttgatt taccggtaat agggattatc aaaagagatt
     2641 acgatgggtc agaagtgttt attactgcga cagaaaaaga agtggaagaa cttttggaga
     2701 cgcaagcaga aatcattgca ctggatgcaa cttctcgtaa acgtccgaca gaaaaaacaa
     2761 ctgctgaact tgtccaaatg attcacgaac acggtcggtt agctatggca gatatttcga
     2821 cttttgagga agcgcttcaa gcgcaagaag atggttttga tcttttgtct acgactttag
     2881 caggctatac agaatattca cgaaagacgc agacacctga ttttgattta ttgaatcaaa
     2941 tcatagaaca agtcgatatc ccagtaatta tggaaggaca tactgactct cctgaacaag
     3001 tagagaaagc atatgaatta ggtgcttttt cagtcgtagt tggatctatt atcacccgtc
     3061 cgcaattgat cactaaaaga tatgtcgaag ccgcaagtaa agcaaataac taaaaaacac
     3121 gcacggcaca gattcttgac aaaaaagaaa aattgataga tagtttaaag cagattctaa
     3181 gaagatgaaa aaagcattac ttttcatttt gtcttcaaga tctgcttttt aacgaatttt
     3241 atagaaagaa ggcgaaacca tgtttgaacg gagtccttca gaattgacag aacgtgaaaa
     3301 ttacaagttg ctgattggtt cagtcatccc tagaccagta gcagtagtaa cgactaaatc
     3361 agcaaaagat atagtgaata tcgcaccctt cagttacttc aatatcgtca gttcgaatcg
     3421 acccatctta tccttggcga tccagagaaa acaagatgca gtaaaagaca cagcaaagaa
     3481 tattcttgag acaagagaag ctgttatcca tatccttgat gaggacaacg tagaagaagt
     3541 aaataaaaca gcagccaatc tgcctaatga ggaaagcgaa ctgtacctga ctgacttgac
     3601 tttaactgat tcagtaatca tttcagtacc agcattgaca gaagcaaaag ttcgttttga
     3661 aacatcctta tatgaacatg tcgagatacg gaataaaaat aaagtaacag ctgatttatt
     3721 tttgctagaa gtacagaaat atcatttaaa tgaaacagtc tatgatgata agacaggaag
     3781 gatcgatcca agaaaattga atgctgtaag ccgtttggca ggaaatgatt atgcagcaat
     3841 aggagaaatt ttcacgattg cacgtcccga ataaaaagga gcactagtta tgttaaaaat
     3901 aagctataaa ccatcgacag attcaaaaga aatgaaaaaa gaatatgaaa cagtgaatga
     3961 ttttctgcaa gggcagtact tagaagttcc gccattgcaa gaccattttg tcgttactac
     4021 tgttacttta gatggtaagg aaatagaaat gccagatcaa acgattagcg gattgtttaa
     4081 ttactttaac aaataaatat ttattccgaa aagtgaaaaa acatataaaa aaacacataa
     4141 aactaaaaaa ttatcaaaga atcctggtgc aactgaaaga atttgcaacg ggattctttc
     4201 aacttatttg ttattttttt ctaaaaaata ttaaagattg ttaaatttca ctatttcatt
     4261 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
     4321 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
     4381 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
     4441 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
     4501 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
     4561 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
     4621 gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac
     4681 aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa
     4741 tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa
     4801 aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg
     4861 attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt
     4921 tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt
     4981 atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag
     5041 tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag
     5101 cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt
     5161 gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa
     5221 ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc
     5281 gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt
     5341 tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc
     5401 aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa
     5461 aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agatcaatac cagaatatat
     5521 acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg
     5581 attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag
     5641 atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac
     5701 ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag
     5761 cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa
     5821 aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca
     5881 aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa
     5941 gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac
     6001 ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag
     6061 ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat aagaatccag
     6121 aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc
     6181 ttcgtgggac aacaggtgag aaattaagca ttacagatgc agacggtgta gagaaaaaag
     6241 ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg
     6301 cacaaaaaac agcttttgac agtctaggag gaaaagctgg atcaactgtt gcgacaacgc
     6361 caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga
     6421 caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca
     6481 tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaataatt acagcagcaa
     6541 tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat
     6601 ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt gatgtatcac
     6661 aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt
     6721 tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc
     6781 ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag
     6841 atatcttgct agctgatact ggatatggac agggcgaact tctaattact cctatccagc
     6901 aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct aaattgattg
     6961 cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg
     7021 tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt
     7081 tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa
     7141 aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg
     7201 tcagcatgtt ggaaaataaa gatgatgatg attcagcaac caaacgagca tcggaactat
     7261 tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact
     7321 gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca
     7381 acttcttttt gatttactca cacttcataa actttttttg taaaatattt gagaacaagt
     7441 ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg
     7501 atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc
     7561 ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa
     7621 taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt
     7681 tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata
     7741 aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga
     7801 ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct
     7861 acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac
     7921 actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa
     7981 acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc
     8041 cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca
     8101 gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact
     8161 tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt
     8221 cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca
     8281 agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc
     8341 agaccagcta aaaacattaa taagaacaca ccaatctccg aaaagtcatg gactagaata
     8401 tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca
     8461 atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat
     8521 atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa aatatagccc
     8581 gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac
     8641 aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat
     8701 gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg
     8761 aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac
     8821 aataagacga atccgccaat caacaacaga atactactat aaacactcca acttttagct
     8881 tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga
     8941 agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc
     9001 aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg
     9061 taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg
     9121 ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca
     9181 gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa
     9241 aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa
     9301 tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt
     9361 tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat
     9421 cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat
     9481 tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt
     9541 ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta
     9601 tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg
     9661 taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact
     9721 tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa aaggattgtg
     9781 acaaaat
//
LOCUS       DAYYAJ010000110         9635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000110 DAYYAJ010000000
VERSION     DAYYAJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.110"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1207
                     /locus_tag="ACHATZ_002338"
     CDS             83..1207
                     /locus_tag="ACHATZ_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8404822.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1434..2351
                     /locus_tag="ACHATZ_002339"
     CDS             1434..2351
                     /locus_tag="ACHATZ_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404823.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2344..3177
                     /locus_tag="ACHATZ_002340"
     CDS             2344..3177
                     /locus_tag="ACHATZ_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404824.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3260..4378
                     /locus_tag="ACHATZ_002341"
     CDS             3260..4378
                     /locus_tag="ACHATZ_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8404825.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4399..6270
                     /locus_tag="ACHATZ_002342"
     CDS             4399..6270
                     /locus_tag="ACHATZ_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8404826.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            6264..7487
                     /locus_tag="ACHATZ_002343"
     CDS             6264..7487
                     /locus_tag="ACHATZ_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8404827.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7573..9507)
                     /locus_tag="ACHATZ_002344"
     CDS             complement(7573..9507)
                     /locus_tag="ACHATZ_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8404828.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
BASE COUNT         3125 a         1585 c         1925 g         3000 t
ORIGIN      
        1 aaatatttgt gttactaatt ccgttaatat acataagtga aagcgtttta tataagaact
       61 tgaaaagaaa gaagggatag acatgaaaaa aagatacaaa ttggtgatag ctttgctaaa
      121 cattacccta ttattcagct taagtgcatg tggaaaaact aacgaagaag taacagcaaa
      181 catcgcagat ccaatgataa aaaaagccac tacattagaa gtatatgtcg caaacggcaa
      241 actgacaaac tatgttgaaa atgctgcaaa aatctataac cgcaaaactg gagcaaatat
      301 tgacttaaaa attgtgaatg tagcttcggg aacagcaaca gtacaaatga tcaccccaaa
      361 attggtaaca cacgaagata tgccggatat tatctccatg gcagacactt cagcagcagg
      421 tgtgctggaa aaatttcaag atgcttttta tagtgctgat gaatatggat tttatgacaa
      481 atatggcgat cagttttatg atcaaaaatt gaatattttg cgtcaacaaa cgagtaatga
      541 aacgattatt ccttgggcta gtgattttac cccagcactt tgttattatc aaccaaaaaa
      601 atttgctgaa ataggtgtag attttgaaga aatcagttct tgggaagaat atatccaggt
      661 ggcaaaagca ttaaaagaaa aaactggtag ttttggaatt gccttgccag aatctgggga
      721 ccaagaattg tttatcaaca tgctagccca gcagaatcaa tctttgcttg atgaagaagg
      781 aaatatccgt ctgaatacta aagaagcgaa acatgctgca aagttaatca aagaaatgat
      841 cgactcagat attgtacatt tctacggtgc acaagatgct gagaaagcat ttcaagaatc
      901 tgcaatgttt gttgctggcg gctggtatgc tacgaatatg tctttgaatt ttcctgatgc
      961 atctgacaaa tggcgcatgg ctccactgat tcctttttca aaagaaaatc caggaaaagg
     1021 tgcagtatca ggaggatcaa gtttttatgt ccctaaagat gcaaaaaatc cacaagtggc
     1081 acaacaattt cttacattta tgctgacaga tgaagaatgc ttagccaacg cactggagct
     1141 gggcgtggct acatctaact cattggccta tcaaacagaa gcagctgaga aaaagtttga
     1201 ctactaagga ggacaaaaat actatgaact attgaataaa atcaatgaaa atacatcaaa
     1261 tactgtttta ccagccactt attcagatgc tacgtcttat ttgactaccg ctgcatataa
     1321 ctattggcaa acaggtgatt ttgaaaattc atatctaaaa gaagcagaaa attttgctca
     1381 taagtacggc gtaaaattaa ctggtgatta aaaaatatta ggaggactta acgatgatgc
     1441 aatcttcgtt accaagcaag acaataaaga acagcaagaa aaaagcaaca gatacgtctc
     1501 ataaaaaaag tgcttatgga ttggtcacac cttttgttat tttctttatt gtgacgattt
     1561 tgattcccat cattatctcg tttgctttta gttttatgaa aattggctac tcatttgaat
     1621 ttgttggatt tgacaattat ctagctatct tccgagatcc attgtttatt aaatcttaca
     1681 cgaatgtttt gattatcatg gcaggatcaa ttccaataac gatgctatta gctatttact
     1741 ttgctgtttt attgaattca gcacagttaa aaggaaaagg attttttaga actgtctatt
     1801 atattccaac cattacatca acggtggctg tagcttccgt attcatgact tttttcaatc
     1861 cgactgggtt attcaatgga tttttgaatg cgctaggaat taacggtgta cagtggttaa
     1921 caaatccttt ttggatacga atatcaatga tcatcacgat gatttggctt agtgtaggat
     1981 ataacactat tttattttta gctggtcttc aagggatttc taaagagata tacgaaagtg
     2041 ctgaaataga tggagcatcg aaatttcggc aattcttttt attaacgatc cccatgctaa
     2101 agccaatcat tttaatggcc acagttttag ccactatcaa tggcttgggt aatttcaata
     2161 tcccaaatat attctttggg acttccaatg gaccagaaaa ttcagctttg gtcgtaggag
     2221 tcaatttata taaaacaagt tttgaaatgg ttgattttgg aaaagcctct gctattgcct
     2281 ggacgatggt gtttgtatct gttatattat cggctattca atttaaattc ggaggtgata
     2341 aaaatgacta aacaaaagat acaaagcaga atttttacgt acgtcattat tgtgtttggt
     2401 gccttaatat ttttgatgcc atatttatat atgattgtag cttcaacgca aagtaatcaa
     2461 gaaattttaa gtggcggaat caatttcaag tttggggata tgctattaaa aaactggcaa
     2521 gccatcatgg cgagatataa ttatcccttg attatttgga attcgattgt tattgctgta
     2581 atcagtacag ttttagctac agccagttct acaatggcgg gttatgcatt agcaaagtat
     2641 cgttttatgg ggaataaact gttgttcaat acagttatgc tatctaggat ggtgcctttt
     2701 tttaccacat tgattccttt attttacatt atgagtcgtt taggattatc gaatacttat
     2761 gcaggtgtga tcatcccttc gattgccagt acaacttcag tgtttatgat gcgacaatat
     2821 agcagtcaat ttcctacagc actgatggag gctgccagaa tcgatggtgc caatgagtgg
     2881 gtgattttta cgaaaatcgc tgtaccgatt ttatcaccta cgattattac tagtggttta
     2941 cttatcttta tgggaagttg gaatcaatat ctatttccat tagtcatgct ttcagataaa
     3001 gataaatata cggttccctt aattatcaaa aatatgacgg tttctagtac aggtgagcca
     3061 attaattatg gcgcaattat gctaatttta gcaacttcag tgatcccaat ggtcttgatt
     3121 tatgtgtggg cgcagtctaa atttaaggag aatgacattg gagctgcaaa taagtaaaaa
     3181 aaataacgaa tgttaaccat ctagttcgtt ctaggagcaa ctccacattc ataaaaatta
     3241 aaagaatggc gggatatcca tgaatgaaca gacgaaggaa tggtcaagaa aaattttgaa
     3301 tgtagcttct gaaaagatag atgcggaatt agacagaatg gggaataaaa ttccatatat
     3361 ttcttacaac ggaaaatata cagacgtaat aaaggacaga ggaatcgact ggtggaccaa
     3421 tggtttttgg agtggtattt tatggcagct ctttcacaag acaaaacgag aaaagttcaa
     3481 agaggcagca atacaacaag aacaattatt agaatctgcc ctgtaccatt tttcagacgt
     3541 tcatcatgat gtaggttttt tattcttacc atcggctatt gcacattatc aacagtctca
     3601 aaacaaacat agtcgacaac tagggttaca tgcagctacg gtacttgctg gacgctataa
     3661 tatttcaggg aactatttag tagcatggaa tgaccataaa cctggttgga tgattattga
     3721 tagtatgatg aatttaccat tactgtattg ggcttcgagg gaaactggtg atccgaggtt
     3781 ctctaaaatt gctcggcagc atgcagatac aacagctaag tatctagtga ggaaagatgg
     3841 atcaaccggg catatcgcag aatttgatgc agaatcaggg aaatatattc ggttacttgg
     3901 cgggcaagga tacaatgaga attcttgttg gagcagagga aatgcatggg ctttatatgg
     3961 attcgcgtta agttatcgtt acacaaaaaa tccgtactat ctagctatag cagaaaagat
     4021 agcagtaaat tttattcaga aaatcgaaaa aactggttat attcctctca ttgattttgc
     4081 tgtaaaagat tcgaatattg ataccagtgc tggagtatgc gctgcatgcg gtcttctgga
     4141 attagcagga tatgtttcct ctgtacgatc aaaaatgtat catgaagctg ctatcaatat
     4201 actccaaaag acagaaaagc actatgcaaa ttggaatatg gatgaagatg ggatcattgg
     4261 aggcggagca gaagcatacc atagagaact gacttataat gtgccactga tctattctga
     4321 ttattttttt attgaaggct tacttcgatt agaacagtcg gaattagcta tttggtagta
     4381 aaaagaggag aaaatacaat gaaacatcca gtaccactat taaggaccag acaggattac
     4441 tttgacttat ttaaaaagat aaatcaacca ctccagccgt tttatcgagc aagtggcgca
     4501 caaattgatt atatacatca aggggtagga tatggaaata aaattgcagg aatggaagga
     4561 ttctctcggg ttttatgggg agcggggccc gcaattgacc aattagatga acgatggttg
     4621 aaattaattc tatcaggaat aaaagctgga acagatcctt cccatcccga ttattgggga
     4681 gaaatacatg aaagagacca gcgtatggta gaaatgcccg caatgttatt agcgttatat
     4741 catcatgaac aattattatg gaaaaaatta gatacaggag aaaaaagcca gattattcat
     4801 tggttctcgc aaatttttga atatgaatgt gcagatggta attggcaatt ttttaagata
     4861 attgtcggaa caattatcca aaaatttggt tatccagtta aagaatcagc actgaaagaa
     4921 gcatttgata atatagaaaa ttgttatctt caaaatggct ggtatcgaga cagttcaaga
     4981 ggtcgccaag attattataa tccttttgca tttcattatt atggtttgat ttatagcgta
     5041 ctagaaccag aaaagcccat tagccagata tataagaacc gagctattga tttttctaga
     5101 gactatattc atttttttgc ggaagatggt gcaaatgtgc cttttggtcg aagcatgatt
     5161 tatcgttttg cagtttcaag cttttggtca gctatgttgt gggccgatct tttgcctttt
     5221 aaattaggtg aaatcaaggg gttagtcaat cggaatataa gatggtggat gcaaaaagaa
     5281 atatttgatg aaaaaggtat cctgaatatt ggttatacct accctcaatt gacattgact
     5341 gaaccctata atagtacgtt atctccatta tggctgaata agattttttt attgttggcg
     5401 ctacctgaag accatgagta ttggacggct aaagaaatgc ctatgcctat tcgtgaagga
     5461 actaaacttc tttcagaagc caatttgctc atacaacatg ataatggaca tacatttttt
     5521 ttgaatgcgg gacaattagg gccaaatttc catacattga ccaatgaaaa atatttaaaa
     5581 tttgcttatt ctagtcaatt tggtttttcg ataccgcgag cgaatcaatt gaaagcagaa
     5641 atggcaatgg attcaatgct agggattcag cgagcggaca catatatcac gaccagtttt
     5701 aagaatgaga gcatcaaaac ctatggacag ttcatcgttc gaaataatgt acgagacatt
     5761 gtatgtaaga aagaggtact tgcctctaca tgggaaccta ctttacaggc aaaaatcaga
     5821 acttggctta ttccttttga aggctggcag attcgcgtac acaaagtaga attagataca
     5881 gaatatgttc tatatgagac agggtttgct attgaatgtt ctcctgagaa agaaggaata
     5941 atcatagaag aagacaagga aaactatagg gtgagtgaag ctggcttttc aggtattatc
     6001 tgtttaagtg acgatactat taaacgaaaa tccacagcta ttatgggttt gcccaataca
     6061 aacttgatga catgggaaaa cacttttatt cctggattag agggtacatt tggaaaagga
     6121 acccattggc tcggaacagg agtggttgct catcaagatc gggattatag tttagaagta
     6181 tggaacaatc gtcccaaaat tgattttgat gggacaaccg ggctaactat ccaaaataaa
     6241 aacaataaaa aaagactaaa attatgctaa aggctggtgg ggaaatgctt gataaagtga
     6301 tgtttgaaga gataatacat ccagaaaaat ggataaatga atgttataaa gaattaatgg
     6361 aaaaagaatg gaataatgag atcgagcgtt gtcgcagaca cacggagagg ttaatcaaac
     6421 gtatcagtaa cagtcagtat caattcccaa gcgagtgtcc tataaaagga cgttatccat
     6481 taagagacaa tattggttgg acagaaggat tttggacagg aattaattgg ttgttatttg
     6541 agaagacggg tgatccttta tttcaacaaa cagctgaaaa agcagatata gattttatcc
     6601 acaggattga ctgtcaaatt atcactaatc atcacgatct tggttttttg tattctttat
     6661 caaccgtagc agattataaa ataacgggaa atcgaaaaat gaaggaagct tcaattgatg
     6721 ctgcagaaac attacttcag cgtttttggg agaaggcagg agttatacaa gcttggggag
     6781 atcctagtga tccttcacaa caaggtagaa tgatcatgga ttgttgcatg aatcttccat
     6841 tattgtattg ggcgagtgaa gaaactggaa atcaaaaata taaaaaagct gctcaggctc
     6901 atattcaaaa tgcagcagat tatttagtaa gagaagatgg ttcaacttat catacatttt
     6961 atatggatgt caaagatgga aaaccaagat atggaagtac gcatcaaggt tatagtgata
     7021 attcctgttg ggctagaggc caagcatggg ggatttacgg ttttgcatta agttatcgtt
     7081 atttgaaaga acaaaaatat tttgatttaa gcaaaatatt agccaattat tttatgaatc
     7141 ggttgccaga agataccatt tgttattggg atttgatctt tgatgattct agcagagaag
     7201 aaaaagatac atctagtatg gtgatcatgg tttgtggatt attagaatta atagaatcag
     7261 gtctattaga caaaacagaa aagaacttat accagtctgc tgtttggaaa attatgcgac
     7321 aattgaaaga aagatatgct acaacggaag aagaagatgg ctttttaaca cagtctgttt
     7381 ataataagcc caaaaataat ggggtaaata caccttgtat ttggggtgat tactattatt
     7441 tagaagcttt agtgagaatg actagtaatt ggtcttctta ttggtgaaaa aggtaaatat
     7501 ggaatttttg tttgataaaa taaaaaaaga aaaaccaaaa cagcatgaat ggtttctgtt
     7561 ttggtttttt cgttaatttt catattcttc taattccaag cttagttctt cccattccgt
     7621 caattttgtt tcttgatctt gtctagcagc ttctaaatct tggttcagct cattgagttt
     7681 tacgtgatct tccaggtttt ctggcgcgac catttcttgt tccaactgat cgatccattc
     7741 atcgatccgc gccatttctt cttcaacctg agtaattttt cgttgaaggc tacggataag
     7801 cttttgctgt tctttgcttt ggtaataatc agatttgttc gtgggagcag gagcagattc
     7861 tggctcatct tgtgccaaca attcggcgat ttcttcttct tcctgctttt tctctaaata
     7921 ataatcatag tctcctagat agactttgct gccattttca gagagttcga cgacttttgt
     7981 cgcgatccga ttgatgaaat atcggtcatg ggaaacaaaa agcagtgtgc cgtcataatc
     8041 gatcaatgca ttttctaata cttctttgtt gtcaatatct aaatggtttg ttggttcatc
     8101 caaaatcaaa aaattttctt tgttcatcgc taacttggct aaagcaaccc gtgctttttc
     8161 accgccactt agcagaggga ttggtttttc tacatcttcg ccagaaaata agaaagcacc
     8221 taaagcactt cggatctctt tttctggagt ggtaggatgc tcatcccaca attctgctag
     8281 gatcgtcttg tttgtatgca gatcagcttg accttgatcg taatagccga tggtgacatt
     8341 tgtccctagc gtttcttttc cacggataaa agggatctgg tgaatgattg atttcagcaa
     8401 cgtcgatttc ccgataccat tcggacctac tagagcaatc gcttcttgac gacggatatc
     8461 tagattgatt ggttcagata aggtgacatt atcatatccg atagcggcat cttcgatttg
     8521 caagactaca tttccagaag gcttttctat actgaataga aagtttgctt gtttttcatc
     8581 cccttgagga cgatccagcc ggtccatttt ttctaatgtc tttctgcggc tttgcgctcg
     8641 ttttgtggta gaggcgcgaa caaggttccg agcaacgaag tcttctaatt tatggatttc
     8701 tgtttgctgc ttttcataag ccttccattc acttgctaac tgttctgctt tcaaatctag
     8761 atatttcgaa tagttacctt tgtaatggcg catttttctt ctgcttaatt catagacttc
     8821 attgactact ttatctaaaa aataacggtc atgggaaaca atcaataatg caccagtata
     8881 gctttgcaga taattctcaa gccaggaaag tgtctcgata tctaaatggt tcgttggttc
     8941 atctagaatc aaaatgtcag ggtgttgcaa aagcatgcga gctaaagcta aacgtgtgcg
     9001 ctgtccgcct gataaagtat cgattttctg atcataaaat gaagaatcga atttaaaccc
     9061 atgcaatacg gaacgaattt cattttcata accatagccg tttcgatctg aaaaatcgtg
     9121 ttgcagccgg tcgtattctt ttaaataaga actataagca ggtgtgtctg gcgtaccttc
     9181 gctaatgaaa atctctagct cgcgcatccg ctcttccatt tgtctaactt gttcaaaagc
     9241 tttcagcatc tcgttccaaa ctgtttcatt ggaatcaagt cctgtgtcct gagcgaggta
     9301 tccgagagtc gctgttttat ttttagcgat ctttccttca tcaggtgctt caattccagc
     9361 aatgatcttt aataaagtcg attttccagc gccatttctg ccaactaaag cgacacgagc
     9421 gcctgtactg atttctaaat gtatgttttc aaataagact tctgcaccaa aatggcgagc
     9481 aatctgattg gcttgtaata atatcatgga atcctctttt ctactttgtg aattctcctc
     9541 aaataaaaaa ctatttttca attagtagtc taccataaat tccattatac tgcgagattt
     9601 gcagaaatga taaataaatg aataaagaat aaaat
//
LOCUS       DAYYAJ010000111         9484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000111 DAYYAJ010000000
VERSION     DAYYAJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.111"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..916
                     /gene="gnd"
                     /locus_tag="ACHATZ_002345"
     CDS             14..916
                     /gene="gnd"
                     /locus_tag="ACHATZ_002345"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8404829.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            918..1919
                     /locus_tag="ACHATZ_002346"
     CDS             918..1919
                     /locus_tag="ACHATZ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8404830.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1916..2200
                     /locus_tag="ACHATZ_002347"
     CDS             1916..2200
                     /locus_tag="ACHATZ_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8404831.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2233..3030
                     /locus_tag="ACHATZ_002348"
     CDS             2233..3030
                     /locus_tag="ACHATZ_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8404832.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3044..3877
                     /locus_tag="ACHATZ_002349"
     CDS             3044..3877
                     /locus_tag="ACHATZ_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8404833.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3895..4752
                     /locus_tag="ACHATZ_002350"
     CDS             3895..4752
                     /locus_tag="ACHATZ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8404834.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4774..5283
                     /locus_tag="ACHATZ_002351"
     CDS             4774..5283
                     /locus_tag="ACHATZ_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8404835.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5320..5721
                     /locus_tag="ACHATZ_002352"
     CDS             5320..5721
                     /locus_tag="ACHATZ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404836.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5833..6186
                     /locus_tag="ACHATZ_002353"
     CDS             5833..6186
                     /locus_tag="ACHATZ_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404837.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6220..6663
                     /locus_tag="ACHATZ_002354"
     CDS             6220..6663
                     /locus_tag="ACHATZ_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8404838.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6680..7765
                     /gene="uxuA"
                     /locus_tag="ACHATZ_002355"
     CDS             6680..7765
                     /gene="uxuA"
                     /locus_tag="ACHATZ_002355"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8404839.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7758..8471
                     /locus_tag="ACHATZ_002356"
     CDS             7758..8471
                     /locus_tag="ACHATZ_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8404840.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8678..8827)
                     /locus_tag="ACHATZ_002357"
     CDS             complement(8678..8827)
                     /locus_tag="ACHATZ_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404841.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9223..9423
                     /locus_tag="ACHATZ_002358"
     CDS             9223..9423
                     /locus_tag="ACHATZ_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8404842.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3182 a         1496 c         2107 g         2699 t
ORIGIN      
        1 tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc
       61 tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt
      121 tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac
      181 ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat
      241 gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc
      301 ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt
      361 ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg
      421 aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag
      481 aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg
      541 aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga
      601 gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg
      661 ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg
      721 gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc
      781 tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga
      841 tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt
      901 tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg
      961 gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta
     1021 ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca
     1081 ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc
     1141 tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac
     1201 accagacaat cgtatctatc atacctgctt taattgaacg aaatgctgta gcagaaaata
     1261 atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg
     1321 acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg
     1381 gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc
     1441 gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg
     1501 caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga
     1561 atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa
     1621 atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg
     1681 gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac
     1741 gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct
     1801 acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca
     1861 gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat
     1921 gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga
     1981 aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt
     2041 cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga
     2101 aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg
     2161 aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa
     2221 ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct
     2281 actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga
     2341 caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc
     2401 caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca
     2461 aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg
     2521 aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa
     2581 gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt
     2641 atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag aaatgcggca
     2701 gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat
     2761 ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa
     2821 atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg
     2881 ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa
     2941 gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg
     3001 gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt
     3061 agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg
     3121 aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat
     3181 catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg
     3241 ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt
     3301 accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt
     3361 cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa
     3421 atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct
     3481 ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt
     3541 aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat
     3601 tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat
     3661 cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa
     3721 atctttagat attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt
     3781 tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc
     3841 ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct
     3901 gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa
     3961 gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg
     4021 ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct
     4081 ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc
     4141 ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa
     4201 gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg
     4261 gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca
     4321 atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc
     4381 tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc
     4441 cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga aatgctatca
     4501 gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca
     4561 ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg
     4621 ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga
     4681 ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc
     4741 ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg
     4801 gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat
     4861 tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa
     4921 aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc
     4981 tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa
     5041 acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt
     5101 gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca
     5161 gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa
     5221 gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca
     5281 taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt
     5341 cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa
     5401 cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc
     5461 attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa
     5521 gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc
     5581 atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt
     5641 ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta
     5701 ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag ctactttttc
     5761 ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca
     5821 tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg
     5881 tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa
     5941 aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct acagttttcg
     6001 ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa
     6061 aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa
     6121 acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt
     6181 cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt
     6241 acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg
     6301 agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag
     6361 gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta
     6421 attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag
     6481 aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat
     6541 catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa
     6601 gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt
     6661 tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc
     6721 gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct
     6781 gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt
     6841 tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa
     6901 ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt
     6961 gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa
     7021 aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat
     7081 cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt
     7141 caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta
     7201 tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat
     7261 tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga
     7321 actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc
     7381 agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga
     7441 aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag
     7501 aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg
     7561 ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat
     7621 acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta
     7681 tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa
     7741 accactggga gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt
     7801 tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt
     7861 atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag
     7921 aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca
     7981 atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag
     8041 aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata
     8101 cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa
     8161 tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt
     8221 gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga
     8281 tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt
     8341 tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata
     8401 aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt
     8461 tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg
     8521 agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga
     8581 acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga
     8641 gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt
     8701 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
     8761 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
     8821 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
     8881 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaatttcaa
     8941 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
     9001 tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc
     9061 attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg
     9121 taatttgagc gatatatcta catagataaa actccttttg cctatcaaat atttattgca
     9181 acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta
     9241 aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta
     9301 tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca
     9361 gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct
     9421 taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa
     9481 aatt
//
LOCUS       DAYYAJ010000112         9404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000112 DAYYAJ010000000
VERSION     DAYYAJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.112"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(402..1271)
                     /gene="scrK"
                     /locus_tag="ACHATZ_002359"
     CDS             complement(402..1271)
                     /gene="scrK"
                     /locus_tag="ACHATZ_002359"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8404843.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1291..2412)
                     /locus_tag="ACHATZ_002360"
     CDS             complement(1291..2412)
                     /locus_tag="ACHATZ_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8404844.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2414..2809)
                     /locus_tag="ACHATZ_002361"
     CDS             complement(2414..2809)
                     /locus_tag="ACHATZ_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8404845.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2828..3625)
                     /locus_tag="ACHATZ_002362"
     CDS             complement(2828..3625)
                     /locus_tag="ACHATZ_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8404846.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3627..4415)
                     /locus_tag="ACHATZ_002363"
     CDS             complement(3627..4415)
                     /locus_tag="ACHATZ_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8404847.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4429..4905)
                     /locus_tag="ACHATZ_002364"
     CDS             complement(4429..4905)
                     /locus_tag="ACHATZ_002364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8404848.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4977..7634)
                     /locus_tag="ACHATZ_002365"
     CDS             complement(4977..7634)
                     /locus_tag="ACHATZ_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8404849.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(7698..8057)
                     /locus_tag="ACHATZ_002366"
                     /pseudo
     CDS             complement(7698..8057)
                     /locus_tag="ACHATZ_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(8235..8555)
                     /locus_tag="ACHATZ_002367"
                     /pseudo
     CDS             complement(8235..8555)
                     /locus_tag="ACHATZ_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(8671..>9404)
                     /locus_tag="ACHATZ_002368"
     CDS             complement(8671..>9404)
                     /locus_tag="ACHATZ_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404850.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT         2971 a         1885 c         1316 g         3232 t
ORIGIN      
        1 tagtataact taaattttaa ataaatcgtt atttattttt actctcactt aaaaaagcac
       61 taaaattata agagagggag gtaatggtct gaacaccaag aattgttcca acatcgaatt
      121 gcttaactaa tgagttcaaa gcttaattat gtggctctta tatttaccga gcgtggattg
      181 cgatctcttt agcgaaacaa acatctttca taaaaaaaac agccatatcc gctttcttgg
      241 atatgactat ttactactac tttactatct ttcattcttc ttgtctcact tatgataagg
      301 cgctgggtat catgagtgaa tgatattgct gaatacaaat agtccgacca cattaagttg
      361 atcggacttc ataagcattt tattgactaa tacttttttg cttaagaatt gattctaaca
      421 tcttttgcta aagttaagca tcctatagtt cctgcattat ctgctaactt aggtgtgaca
      481 atgtattctt cgatatttgg cgtatcgaca tagccgttaa ttagttctaa aaaacgtctt
      541 tgtactttct ttttcagatg ttcttgttgc atgacaccac caccaaaaat tataatatct
      601 ggtgaaagca tcagggtcgt attacaggca cattgtgcaa tataatccgc ctcgatttcc
      661 cagaacggat cttctataga aagatcttgt cccttggttc ccgtacgcat ctcgatagcg
      721 ggtccagatg ccaacccttc gagacaatca tgatgaaaag gacaagtacc agagaaagta
      781 tctcctgaat gtctcttaac taacgtatgc cccatttctg gatggctaaa cccttcaaca
      841 aaaatcccat cttgtatagc tcctccacca attccagttc caacagtgta ataaacaaca
      901 ctggataaac cttttccatg acctgaaaca tactccccgt aacaagcagc attcacatcg
      961 gttgtccagg caattggaat ttcgaaatgc ttattcaacg ttccaacaaa atcaaaattt
     1021 ttccaggcag tttttggagt agaggtgata tgtccatagg tctttgattc acgatggata
     1081 tcaatcggtc caaatgaccc tactccaatg ctttctaatt gttctttata gtgattaaag
     1141 aaatcaatta ccaaactcat tgtttcattc ggtgtagttg taggaaaact cactctttct
     1201 aaaaccttta aatcatcatt gccaatggca caaacaaatt tagtcccacc tgcttctatt
     1261 gagccataca tttcttactc caccgtcctt ttatctagga attcgttctt ccctgtctaa
     1321 tagtttttcg aaaaactcat gtggttcctt ttggtaccaa taggcaacag aagaaatatc
     1381 gtcttgacgc tcataaagct tgatgtcatc atttcctagg gtttggaaag taacacgtaa
     1441 attttctttg aaactaatgg ggtcgggtaa atgccagcgg tacaacccat gcatcggtaa
     1501 agcatcatcg ccaaaaccat gaactggatt tggattaggt tttccagcac taaaataatc
     1561 ccgggtgtga tctcttttag attgaaaagg atagcccata aatagcgtat taaaattttg
     1621 agcatatgcc ctttctccaa agttcttttt atggaaagcc caagcgccgc caaaataatc
     1681 ttctgctcct gtagatgtta ttgtagggaa atcttcatct ccatctatat aaaacttaaa
     1741 ttctccttcc ccccaccagt atctttccaa tgcgcacaaa gcaaagtagg ttcctacata
     1801 atacccagtg cctttgattc catcaataag ggtgtaatct tttccttttg tcgtgatggg
     1861 ttcacggttc catttagcat gaaaatacat cgcatcatcc ggtacttcag tcaatgcata
     1921 attaaatgta taaaaaatgt gcttaataaa ctcaggatgt tcattcgtta aagtaatctt
     1981 agcttttttt cgaaatggca tctcaaaata gcaattcatc cctgatgttg gattcactac
     2041 gattggtaag gaattcactt cgcatcttgt cccaaaccca ttacaaaaaa aatctcctaa
     2101 tggactttct acagaaggag tggattcatc atcccaataa atacgcaaaa ttaaatcacg
     2161 taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc atccaaaaat gacgtaaaat
     2221 tccagggcct tccatttctg ctaaagtaat tgtctctcca gcatttatcg gaacagaagg
     2281 tgaccctttt ctcccaattc ctagatgact cgctgtcatt gccgctttcc ctttttcacc
     2341 atttttattt tctggtgaaa tagttctact ttcctctccc ttgtggagcc atatattatt
     2401 taaacaattc attttattcg tcctctatcc tttcttcact tttttgatta ttcatataga
     2461 tgatttgttg ttgtgcctcc gcaataattg ctccaatatc taaattatca tttcctaact
     2521 gcaaagcgat tgacattgcc aagggaagat tcattcctgc aattacgtgc actctgtcag
     2581 aaatataggt aaaaaacttt tgatttacac ttcctccagc catgtcagtt aaaacaatta
     2641 tttcgtctgt agtatccact aactctttaa tttgttcatc tatatcatca atttcttcga
     2701 catatgcaca taagctacta atttgattat gcatacctgt taaaaactct agtgtctctt
     2761 taaaaccctc agctagtatt gaatggctag caatgatcaa tcttctcata attcaaatcc
     2821 ttttccttta cgctaatatt ccaaatgcag ccataaccat cgcaaaaaca ataacgagta
     2881 gaatcaaaat ggtcatactg atttttttct tactgagtag ataatatgct gcagcagtaa
     2941 taattactgg aattaatgtt ggtaaaattt tgtccaacat tggttggatt ttcatactaa
     3001 cttcaccaaa agtgaattta agtggcgttg aaacactgat aactgaagca attaatgatc
     3061 caactactgt tagccccaat actgaagcag cttcagttaa ataattaatc ttttcaccgt
     3121 attcagtaac aatcttcatt ccctgcttat aaccaaattc aaagcctctc attcggaaaa
     3181 agaagattac catgattaaa atgaaccata aaaagattcc tacaggatta ttttctaaag
     3241 ccatataagc cgcaattgat cccatgatgg ttgggatcat cgtcatcaaa agagagtccc
     3301 caataccagc aattggaccc atcgtaccaa ctttaaagtc attaatcgca tcttttgctt
     3361 ctaatccttg cttttcctcc attgcaacac aagcacctaa aataatggct gccagccaag
     3421 gctgtgtatt gtaatactga aagtgattat ttaaagcttc gatgtattca tcatcatctt
     3481 tgtataattt tcttaaaata ggtgacaatg cgtaagtcac agatgcacct tgctgtgttt
     3541 gataattaaa ggtacaaact gacatcaacc aacgatagtt cgctttccta agatcctttt
     3601 tcgttacttg atatttttcg ctcattttat tcatcctccc caataaaccc aacatcagaa
     3661 gctcccgcag aagcagtgat ttgtgcatct ttttcagctc tatgatacac attaaatgct
     3721 aaagcgaatc ctatgatcgc cgctccaata ataggtacct tcaaaaaagc ggctaagaaa
     3781 aatccaacaa gtaaataact aatgtatcga cctgtaggca taaagcgtaa caacatcgca
     3841 ataccaacag caggcaacat ttttccggct aatgttagcc catccataaa ccaattaggt
     3901 aaaacatcca aaacgtaatt aacagcagac tgtcctaaag taacggcaat aaatactgga
     3961 attgctgcag ataaaccaaa gaaaattggc cccaaccaca aaacactatt cattttctta
     4021 aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa caaaaccatt taagattttg
     4081 acaattacat ctagttgcac ccctaacata cctacaggta gtcctattgc taatccggct
     4141 tctactccaa gctggctacc aaatgccgtt gcaataattg cgcacgttcc atagtccggt
     4201 acagatgagc caccaaaacc ggcaactcct aatgacatca attgaactgt accgccaact
     4261 atcaaacctg tttttatatc acccatcaaa attcctgtga ataatcccca aaaaactgcg
     4321 taatttacaa agatcatcgg aatcccataa taatcaacat gtttaataaa tgctaatata
     4381 gtcagcataa taatttgaaa aatagtaaaa tgcattatcc ttactccttt atcctaattt
     4441 tgattgaatt tcctgtgcct tatccgccgg aacaaattgt gtaacaattt ctacaccaga
     4501 atccaacaat tttttatacg tgctgatatt ttcatctgat gcataggctc tagaagatag
     4561 ctgtaatgca ttatctttaa caacagtacc gccaacaatt agtcggtcaa ttctaatacc
     4621 gttatctact aaatgaagga ttgtttccgg ctctttcacg atgacaaata ctttttcctg
     4681 atcgtatttc ccctttttaa aattcgcaat cgctgtttct tcagtgataa ttgatacagc
     4741 cattccagca ggtctagcta aacgcatact tgatttttgg atgtcgttat ttgcgacaga
     4801 atcatcgatc accatcactc tgtttgcttt agacattggt gcccattgcg tgacaataat
     4861 cccatgcaat aaacggttat caactctcga cataacaata ctcataaata cttcctcctt
     4921 tatctttcat tttcttacaa gcaattacca tgccaaaaca aaaagaactt gataaatcaa
     4981 gctcttttta cataatcata aacgtattta acctcatgaa tcgggatgtc gacactataa
     5041 taatgttcaa cgacactgaa agattgctta aaatgtgtct gaaatttttg aaattcctca
     5101 ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt gttttactaa tcgttcaatt
     5161 aagcaactaa tgtgaacata taatccgaaa cacgtattat tatcaaatga aattcctaga
     5221 tcattttgta agatgtatat agcatctgaa acccgttcta ataatttagt cggatttaat
     5281 atggtcaatt gctccattaa attcgttaga ctaaaattct tcagaatatt attgttcaaa
     5341 actttcagtt cattatcatt aaagtgtcta ttcaattgat tggagaattt tcttatattc
     5401 attcctaaaa tcatatcttc aattgaaaag aattctacat cttctagatg tggatttagt
     5461 gttccaacaa taaaaagaat attgttctct ttacgaagct ccgataaata gttttcattc
     5521 actaaagagt aatagtcaca cgtttctatg acaagatcag tatcccttgg aaagctatct
     5581 tcaagtatcc tttttaattt aaacgaagtt cctaatccgc ttgcacaaga acagacaatc
     5641 atatccttct taatatttga tgattcataa atataatcca cattttgatt ttcttttact
     5701 tgttcaagaa tctgtttaag cggtaaatct aataatatct gatttgcaac atcaatagcc
     5761 attcttgttg aaacattgtt tagtagtccg aatttttttt gagcgtctag ttccaaattt
     5821 agataaattt tctccagtga acccatatca acaagtaaaa taatttcttc atatccggta
     5881 cactcttgta agtatctatt aattttgtct gcaatctcac tacttgaagt cattagtggc
     5941 atatcaatgg cgtcgtatat atatctctca acgatcctat tagcagtact tgctatacag
     6001 gttgccgtta gatctccatg agccaaaatc agtgctaaac gtttttgctg aaattcttta
     6061 ctacacatta acttaaataa aataaccaaa acattttttt gaatttttgt taaataaata
     6121 tcccctattt gaataaaacg attgactaat tgaacttctc gactacacca actattaata
     6181 tcattgatga aggaatataa tttatcgtcc gatgttaatt ggatttgata aatccaagaa
     6241 ttcaaaaaga tagacgttat ttgaagataa taatagtcaa attctatttg aaaatctctc
     6301 tctatttcct taacatactg tctaaatcga atctcttgat aggtgggata tgttaaagct
     6361 gaattatttt ggatatcaac attcttttgt ctaaaatatc tatcatattt ctcaaaaagc
     6421 tgctgcttat cagtgtcttt cttccacgtt tgataaagat actctataag ttcgttgata
     6481 ttaaaaactt cgtctacttt ttccaaatca tccatttgaa tatattttaa ttcattctgt
     6541 cttttcataa ccttcatgcc gttgaaattc acccattgtg gtaaatgata catctgaata
     6601 taaagttctt ccaaatcatc taagaaataa aaggcattgg cacaggtcaa ttgaatgata
     6661 tttttcaaat tgccaacatt ttcactaaag cgatagttta ataaaaaatc taataattgt
     6721 tgagatatca gtattttctt tgctagtctt tcttcttctg atttaaaaaa tatcttgatt
     6781 aactgttttc tttcataaag agaacgactg tctaatgttg gaagtgttag ctttacaggt
     6841 attcttctca acaaagttga taatagttta tcctcaggtg gttctgatgt tgcaaatata
     6901 atccagcaac ttgatgtgta ccagttttca ttatcaccca tccgatgaaa tttcctcgta
     6961 tccataaact gaaaaagttt ctcctgacat tcattgctca atgcatgaat ttcatctaaa
     7021 aataagactc cattattagc taattctgct aaaccctttc ttccatcttc ggctcctgta
     7081 tatgcacctt ttacatgccc aaaaaaattg gacaagaaaa gttctggatt attggcatat
     7141 tcactacaat taactgaaat gaaagatgcc ttctcttcta tataattatt ggccaacaaa
     7201 tccttataca cataatttgc taaatagctt tttccagttc cagtctcgcc atgaagtaaa
     7261 atgggtaagc catttggcgg atagtagatt gctgctttaa tttgagcaat tacatttcgc
     7321 agacttcctt gacttccgat aaccctttct attccaaccc taaaagagct atcataccat
     7381 ttttccgctt cctctgaaga gctaaaaatt tgttgatttg gaactttctc cggtaaagaa
     7441 aagaagatta ctggacgagt attgattttt ccaaattctc catcttttac gaattcattc
     7501 aaatattgag agacaatatt acgactaaca tttaattttt cagatatttt ctgtgctgta
     7561 ttgttccccc atttattttt aatctcagag ttctctatga cataattgac aatgcgttct
     7621 tttaattctt ccatcacagc acctcctcga cactattcga attttgccga cagtattttt
     7681 ccataaatat gacttgctaa aaaggctttg tttagatcat tcaattggaa cgttgcttgt
     7741 tttgcttgat ccgctaagac gttgaaaaga tactctggcg aaatcgcata ccccaaaata
     7801 tcaacgattg ttgcaatcag atcttctttt gattcttcat ctgaaagtaa ttcacgatac
     7861 aacatcgttt gtttgcttac tgtgtcatat tcctctaagg attcatctgc taaaagaacc
     7921 acttgttcca acaaacggtc ggataaatat ttatagaaaa tcagccccaa taaataattt
     7981 ttatattctg acgcatccat tttactacgg agattatctg ccgcactaaa taacttctga
     8041 tttaattctg cacccaaaaa gatacactcc tatctcttat ttaaagttgc tatataccct
     8101 cttattttag catactcagt tttagaaaaa gcaaaaaaat aaagaaactt attcttgata
     8161 acaagctacg tgacgtatat ccctaacaca aaaatagcca tatcctttgg tttggatacg
     8221 gctatttgat attactcatt caattatcaa atcacagtag gtgtgaatct catcgggatc
     8281 ggcaatatct acttctgatt aggatcaaag gtgattttct caataaaggt gtgatacaaa
     8341 gctttaactt gcttcttctc actctggata agaaagcaat ccaaactagt caatatctgt
     8401 tggacatctt tgacttgaat cggttcccct tgttgttgaa agatccctaa gatttcattc
     8461 tctcgaattt gtaactgtct tcgtttttca gtcaattcat ttaaacgatc ttttaacatc
     8521 ggaaacaatt cgggactatc ttccaaagca gcttcctgtt tttgctctaa tagttagatt
     8581 ttgcgtacca ttttcttctt ttattttcct tcgattgctg attttaatat agtctaaagg
     8641 aaatttatat ttattgtatt ctttataact tcaagagaac ttcgtttgag tattactgta
     8701 tttttttaat tccataagaa aattagttat tacctgccac ctaatcagca aatctgaacc
     8761 tgagtcaata taattaatat tattttccat ctccaacatt gtaagtactg ttggtgttaa
     8821 tatagggact ttgtacaatt gtattttttt gattttatca tctgcacaaa ggagattttt
     8881 taaatctaat ccttctttta tttcaaaagt tgaatttttt aaatctataa gagtaaaacc
     8941 aattccttca tactgaattt ctgaaaatcg aatttttctc ccagttgata gatcaagggg
     9001 cagaaaacct tttattacat tgtgagtgtc accatcattt acaatgtcat taggaatttt
     9061 gattttagaa ttctgtggag cacctattgg aagataataa ttcattataa ttttgtagtc
     9121 ttcatgagga aataaagtac ttttaggatt gctaagatat tctcgtaccg aatctaagaa
     9181 tggcttatcc attgaatctt catccccatt taaactaaaa aacattccta caattgcttt
     9241 tgcaactcta agtgaattca ttttctttat tgaatgtgtt aaatcagact taccattctt
     9301 taaacttgta ttgttataaa ggaaattttt attgtcttct gcaaatctaa tatattcctc
     9361 aacatactca ctaccaaaaa aactattaca tttactacat aacg
//
LOCUS       DAYYAJ010000113         9349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000113 DAYYAJ010000000
VERSION     DAYYAJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.113"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..459)
                     /locus_tag="ACHATZ_002369"
     CDS             complement(145..459)
                     /locus_tag="ACHATZ_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404851.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            745..984
                     /locus_tag="ACHATZ_002370"
     CDS             745..984
                     /locus_tag="ACHATZ_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404852.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1346..1606)
                     /locus_tag="ACHATZ_002371"
     CDS             complement(1346..1606)
                     /locus_tag="ACHATZ_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404853.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1791..2288)
                     /locus_tag="ACHATZ_002372"
     CDS             complement(1791..2288)
                     /locus_tag="ACHATZ_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8404854.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACHATZ_002373"
     CDS             complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACHATZ_002373"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8404855.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACHATZ_002374"
     CDS             complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACHATZ_002374"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8404856.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5011..5686)
                     /locus_tag="ACHATZ_002375"
                     /pseudo
     CDS             complement(5011..5686)
                     /locus_tag="ACHATZ_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(5696..6565)
                     /locus_tag="ACHATZ_002376"
     CDS             complement(5696..6565)
                     /locus_tag="ACHATZ_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404857.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            complement(6576..7472)
                     /locus_tag="ACHATZ_002377"
     CDS             complement(6576..7472)
                     /locus_tag="ACHATZ_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404858.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            complement(7525..8784)
                     /locus_tag="ACHATZ_002378"
     CDS             complement(7525..8784)
                     /locus_tag="ACHATZ_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8404859.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            complement(8813..9127)
                     /locus_tag="ACHATZ_002379"
                     /pseudo
     CDS             complement(8813..9127)
                     /locus_tag="ACHATZ_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            9184..9330
                     /locus_tag="ACHATZ_002380"
                     /pseudo
     CDS             9184..9330
                     /locus_tag="ACHATZ_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         2936 a         1925 c         1434 g         3054 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     1621 taatgttttt tcattattta aaatgataat aacattaaaa tatgattatg aaaatcaata
     1681 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1741 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
     1801 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
     1861 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
     1921 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
     1981 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
     2041 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
     2101 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
     2161 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
     2221 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
     2281 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
     2341 tgtaaacgct aggttttctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
     2401 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
     2461 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
     2521 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
     2581 tgtgtatcat cgataaaatg caggtgaaat cccgctgcag caactccttg gaataattca
     2641 ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc tggttgagac
     2701 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     2761 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     2821 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     2881 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     2941 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     3001 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     3061 gttccatcca tcaagccggc catcaaagct cctaatgtcc cgtgctggta taagattttt
     3121 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     3181 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     3241 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     3301 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     3361 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     3421 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     3481 tcgccggaaa ctgatacgat ctgtgtattt ggacgcacca atgcagcaga aatcgcccat
     3541 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     3601 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     3661 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     3721 tctccttttg attgaacaat gtcccgctcc gttaattttg cttgaagaga tgctaaatat
     3781 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     3841 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     3901 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     3961 tcgattggat catagccaat tgcaataact agatcacttc gtttcaaaag catatctcct
     4021 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     4081 actccagctg cttggaatgt ctctacaaca ggcaatgctg tcttttcaac taattgcctg
     4141 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     4201 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     4261 ggatcgctca atggcttgat gacattacct tggacaggtg catccactac atcttgcggg
     4321 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     4381 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     4441 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     4501 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     4561 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     4621 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     4681 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     4741 tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt cattttttca
     4801 gccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa
     4861 ataagtgata cagtttctaa acctgtttca ttcaaaagta tagcatgttt tatttcattt
     4921 gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa aaaactgtca
     4981 ttcttaattg aaagtttcaa tttataaagt ctacagatcc atagttctca tcaatttacg
     5041 attgttgaag taaatcaaac caaatacgat agaaggacct agtagaatac accattgggg
     5101 ctttacgaaa aagaagagaa acaaacttgt caatagtaaa aaatctagta aactcacata
     5161 ccattttttc aacgtatagt agaacgctat ccgtagaact tgatttggtt tcgacgctgg
     5221 atttctcacc tgaaaatacc agcagttgat gctaaaggaa actgataaac aagcaagtaa
     5281 aacaagcaag taaaacaaac aacggaatca accattttcc ataagaaaaa cgtataaaaa
     5341 agaaacaatc aaaaatcatt atcacactaa ctatccaagc aaatagccaa taaaaaaatc
     5401 cctttagcca aaattggcga aacgtcttta tgaacaaacg tactggtgaa aaactttttt
     5461 cttttgctaa ttcttctgtc atacctatca tcacgatgca acctggacca ataaaaagta
     5521 aagaaaaaat aaaggaaaag acattccttc catctatcga caaaaggaac gcagctgcta
     5581 tatacaagat attgacacac caaaaacaaa ggctacttgc caatgattga tacaaccaat
     5641 aaaagaattc catataaaca tttttgtcaa aacgctgctc tttcataatc acctcctatt
     5701 ccttcattcc tgatgaagcg atcccttcta caaaatattt ttgcatcgct aaaaatataa
     5761 aagcaacagg aagaacagaa acaactgagg cagccatgat taatgcgtac tctgcatcaa
     5821 atagcccaat aaacattttc aaccctaact ggattgtttt attttccgta gaagacaaat
     5881 aaatgaatgg ccccatataa tcgttccatg tattaacgaa tgtgatgatc gtcaaactgg
     5941 caatcgccgg ttttgtaaga ggtaaaatga tcttgcgata gatttcccac tctgataagc
     6001 catcgatccg cgcactctca cataaagaat ctgggatact actgtaatat tgcttcaaca
     6061 agaacacacc aaaagcattg aacgcttgta atagcaccaa cgaccacaac gtatctgtta
     6121 aacccacttt tctcatcata atgaactgcg ggatcatata agactgccaa gggacagcaa
     6181 tcgtgccgat atatgctaaa aataaaatat cgcgcccttt aaaatttagt tcagaaaacc
     6241 cgtaaacagc aaaactagaa gtaaacaact gaatcaacgt gatcacaacg cttaaaaaag
     6301 ctgtattctt aaaaaatgtc actaaaggaa tacgagacca aataaccaga taattctcta
     6361 aatgccaagt tttagggatc cattgaatcg gaatcgttaa cacttcatta tttgctttga
     6421 atgaagcaga aaccatccat aaaaaaggaa tgatcgtcaa taaagctaat acaatcagca
     6481 gaatcatacc caaccattga ataaagggca tgttttttga acgaattttt gctttttttc
     6541 tttcatttcg aacactctct tccatatcac acctcctaag tacgacgctt attccaagaa
     6601 aattgaacaa ccgtaacgcc caaaacaatt acgaacaaaa caagtgaaat agctgaagca
     6661 taaccaaaat caaatttttt gaatgcttca ttgtagattt ggtaaaccag cacatttgtc
     6721 gctcgaccag ggccgccatc cgtcatcact tgcacaaggt caaacacctt gaaacaattg
     6781 atgacaagca tgatcgttac aaagaaggtt gttggtctca acgaaggtaa tgtcacattc
     6841 aaaaattgtt tccaacggtt tgctccatct agcgaagacg cttcatatag ttctttttgg
     6901 atactttgta gtcctgcaag gtatagaatc atgtaataac ccatccctcg ccaaatactg
     6961 acaattacaa ttgcccaaag cgcccaattt gaacttgaag tccaacctgg aggatgttca
     7021 ataaatcgtc ttaaaaagat attaataggt cccactgttg gatgaaacaa catattccaa
     7081 acaacagcaa ttgctactaa ggaagtaaca tacggaaaga aaaatatcgt acgaaaaagc
     7141 ttcatccctc taattttctg atttagtagt aaagctagcc ctaacgaaca aacaagtgtg
     7201 ccaggcacta cagcagcggt atataaaaaa gtatttttca aagaaatcca aaatgtctca
     7261 tcgttggcca ttttacgaaa gttatctagt ccagcaaatt ctggcgttga aaaagaatcc
     7321 catttcataa atgctagtac aagcgaaaac aggattggta ttaatgtaaa aacgaaaaaa
     7381 ccaatgaaat tcggggcgat aaacgaataa gcgatccacg ttcgttttct acgcaaatga
     7441 tttgtagctt tcttatttat actattctcc atttcgatga cgaaaccaag agtgttttat
     7501 ctcttggctt ctctcctttc atgattattc tttttccgca gctacacgtt tctccatatt
     7561 taccgttcct ttttccggcg tttcatctcc aaccaaaact aagtcatatt cttcttgtaa
     7621 aatcttatcg attgcaggcc catacgtatc aatcggaaat tcaagtttga tttcatcagg
     7681 agaaaaagct tttttagaaa tttcatccgt tggcattcca gcgagattaa aataaagctg
     7741 atcaatctca tccgtacgat atgaaggaac tactcctact cctgctaata cttttgctcc
     7801 ttcttcactg gctgcaaatt caataaattc ttgcgccgct ttttgtttct ttgagttttt
     7861 attgacagca aatgctgttg gtgagccaaa cgtcgtgacc gattctcctt ttttcttctg
     7921 aggaatagct gtaattcccc attctacttc tgtcttactt gcttctttgt ttgcgagtat
     7981 gccagccata taccaagttc ccatgtacat cattgctgct ttttgttctt caaattgaga
     8041 ctgataggtt actttcgttg atttagccgt gccaaaatcc atctgcgcgc cttctttttg
     8101 catcctcaat gctcgatcat aataatcagt catatattga taatctggtt caacaagatt
     8161 cgcctcgttt tgagcagctg caatagcttg gattgtcgaa cgccagatat gttgatatgc
     8221 tccgtaaact tgtccgtcat ttttactaag tttttttgct aaatcttcat attcgtccca
     8281 agttagattt tttggatatt cgattcctgc atcatcaaac attttcttat tgtaatatag
     8341 tacccaaaag tccgttcgat atggcaatgc atatgttttt ccatcgatat catacatttc
     8401 atatgtttct tcagcagcat cagtgtctaa ttctctaatt cgttctgttt gatcgactaa
     8461 ctgatctcgt aaagcataat ttgaatacga caaaagattt ttcatcgtca atacatctgt
     8521 cgtgtctcca gaagaaagca tcgtggtgag tttcgtatca taatcatctg aagcaatatc
     8581 tactgctttc actttgattc ccgttttctt ttcgaatgct tcaaagagcg ctttgaattc
     8641 aggtgttgtt tcataattcc atgtcgaaac ggtaatttca tttgctgatt cttcactact
     8701 gtcttcttga ttaccacaag caccaaatac gagtgacatt cctaaaacta ttgatccaat
     8761 catcattttt ttccatcgct tcattatatg aatcctcctc ttctcttata catcaccagt
     8821 aacagtacca tgttttagct agacgagtga ttccttctac ataaaagtaa tcgccccaga
     8881 tcacacattc atctacacct tttaagctgt ttttatcata aacggcatgg agaagcagac
     8941 cgtttgatcc gttaactcgt ttcgttgtat agtttttctg taattcttcc aagattttga
     9001 ttgctatctc ttcgtaatcg aaatgacgag gatctgaaac aggcagttgt ttggaaagtt
     9061 ctagcaatcc acatgccgct atagctgctg cagaactatc tttttcctct ttgctatctt
     9121 ctttgaatct aattttagga ggtacgtttt ggtatttatt cgtacaaata aaagagaatt
     9181 atctttattc atgtaacgca tccaattgat tttttcatgt acgctcctgg aacgatttta
     9241 ctgacgattg cttatttaac tgcgaatcgc tcattagctt ttatcatagc tatccatatt
     9301 ttgaataacg ttcttggctt tgtattatag atacagatga caaaaaaag
//
LOCUS       DAYYAJ010000114         9311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000114 DAYYAJ010000000
VERSION     DAYYAJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.114"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..710)
                     /locus_tag="ACHATZ_002381"
     CDS             complement(42..710)
                     /locus_tag="ACHATZ_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8404860.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            844..1131
                     /locus_tag="ACHATZ_002382"
     CDS             844..1131
                     /locus_tag="ACHATZ_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404861.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1547..3085
                     /locus_tag="ACHATZ_002383"
     CDS             1547..3085
                     /locus_tag="ACHATZ_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8404862.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3082..4047
                     /locus_tag="ACHATZ_002384"
     CDS             3082..4047
                     /locus_tag="ACHATZ_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404863.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4034..5356
                     /locus_tag="ACHATZ_002385"
     CDS             4034..5356
                     /locus_tag="ACHATZ_002385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8404864.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5419..5523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5597..6340
                     /locus_tag="ACHATZ_002386"
     CDS             5597..6340
                     /locus_tag="ACHATZ_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8404865.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6355..7347
                     /locus_tag="ACHATZ_002387"
     CDS             6355..7347
                     /locus_tag="ACHATZ_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8404866.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7359..8096
                     /locus_tag="ACHATZ_002388"
     CDS             7359..8096
                     /locus_tag="ACHATZ_002388"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404867.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8086..8808
                     /locus_tag="ACHATZ_002389"
     CDS             8086..8808
                     /locus_tag="ACHATZ_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8404868.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8892..>9311)
                     /locus_tag="ACHATZ_002390"
     CDS             complement(8892..>9311)
                     /locus_tag="ACHATZ_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8404869.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3050 a         1604 c         1967 g         2690 t
ORIGIN      
        1 tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca
       61 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
      121 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      181 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      241 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      301 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      361 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      421 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      481 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      541 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      601 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      661 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      721 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      781 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      841 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      901 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      961 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
     1021 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1081 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1141 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1201 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
     1261 tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata
     1321 gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga
     1381 accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt
     1441 atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa
     1501 aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat
     1561 acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat
     1621 ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc
     1681 caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc
     1741 ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt
     1801 gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc
     1861 tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg
     1921 cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc
     1981 cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg
     2041 gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat
     2101 cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa
     2161 acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt
     2221 atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga
     2281 acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt
     2341 tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca
     2401 aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga
     2461 agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt
     2521 gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt
     2581 ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc
     2641 tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata
     2701 taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag
     2761 tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga
     2821 tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc
     2881 ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa
     2941 agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca
     3001 agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg
     3061 attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag
     3121 tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt
     3181 gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta
     3241 tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg
     3301 cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg
     3361 acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga
     3421 gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat
     3481 cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt
     3541 gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca
     3601 agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt
     3661 ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag
     3721 gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac
     3781 tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta
     3841 gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt
     3901 gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta
     3961 gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga
     4021 gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt
     4081 aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct
     4141 atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt
     4201 agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc
     4261 caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg
     4321 aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga
     4381 tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac
     4441 cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga
     4501 ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc
     4561 ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa
     4621 tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga
     4681 agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac
     4741 tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac
     4801 tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa
     4861 acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg
     4921 agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta
     4981 tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac
     5041 gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc
     5101 gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga
     5161 accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt
     5221 gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt
     5281 gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc
     5341 ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc
     5401 tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt
     5461 gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac
     5521 gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa
     5581 gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt
     5641 aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc
     5701 tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac
     5761 gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc
     5821 cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc
     5881 acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat
     5941 gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc
     6001 ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga
     6061 tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg
     6121 tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc
     6181 tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt
     6241 ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt
     6301 attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa
     6361 aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa
     6421 acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta
     6481 ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct
     6541 ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt
     6601 gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat
     6661 ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc
     6721 gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca
     6781 ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg
     6841 gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa
     6901 tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt
     6961 attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg
     7021 gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg
     7081 gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg
     7141 ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg
     7201 tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc
     7261 aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa
     7321 ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact
     7381 tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag
     7441 atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt
     7501 taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg
     7561 gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg
     7621 tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag
     7681 taagggatta tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat
     7741 tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt
     7801 acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag
     7861 actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata
     7921 tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg
     7981 tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat
     8041 cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct
     8101 cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg
     8161 acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt
     8221 aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa
     8281 caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt
     8341 tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca
     8401 gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat
     8461 tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa
     8521 caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca
     8581 gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga aacggatgga
     8641 ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg
     8701 cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa
     8761 aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg
     8821 acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta
     8881 ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa
     8941 ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca
     9001 ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg
     9061 taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga
     9121 gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt
     9181 ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga
     9241 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
     9301 tgtcctgagt a
//
LOCUS       DAYYAJ010000115         9276 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000115 DAYYAJ010000000
VERSION     DAYYAJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.115"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..687
                     /locus_tag="ACHATZ_002391"
     CDS             <1..687
                     /locus_tag="ACHATZ_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8404870.1"
                     /translation="WKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENA
                     WTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAG
                     NIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPV
                     VNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDA
                     GAVGGTLYAT"
     gene            677..1417
                     /locus_tag="ACHATZ_002392"
     CDS             677..1417
                     /locus_tag="ACHATZ_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8404871.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1414..4176
                     /locus_tag="ACHATZ_002393"
     CDS             1414..4176
                     /locus_tag="ACHATZ_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8404872.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4189..5097
                     /locus_tag="ACHATZ_002394"
     CDS             4189..5097
                     /locus_tag="ACHATZ_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8404873.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5097..5714
                     /locus_tag="ACHATZ_002395"
     CDS             5097..5714
                     /locus_tag="ACHATZ_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404874.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            5718..6167
                     /locus_tag="ACHATZ_002396"
     CDS             5718..6167
                     /locus_tag="ACHATZ_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404875.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            6167..6661
                     /locus_tag="ACHATZ_002397"
     CDS             6167..6661
                     /locus_tag="ACHATZ_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404876.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            6675..7106
                     /locus_tag="ACHATZ_002398"
     CDS             6675..7106
                     /locus_tag="ACHATZ_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404877.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            7108..7245
                     /locus_tag="ACHATZ_002399"
     CDS             7108..7245
                     /locus_tag="ACHATZ_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8404878.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            7282..7575
                     /locus_tag="ACHATZ_002400"
     CDS             7282..7575
                     /locus_tag="ACHATZ_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404879.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            7572..7796
                     /locus_tag="ACHATZ_002401"
     CDS             7572..7796
                     /locus_tag="ACHATZ_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8404880.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            7793..8818
                     /locus_tag="ACHATZ_002402"
     CDS             7793..8818
                     /locus_tag="ACHATZ_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8404881.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            9008..9081
                     /locus_tag="ACHATZ_002403"
     tRNA            9008..9081
                     /locus_tag="ACHATZ_002403"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9042..9044,aa:Ile,seq:cat)
BASE COUNT         3317 a         1306 c         1922 g         2731 t
ORIGIN      
        1 tggaaaaata ttaaagatac ggcatacaat acgtggattt ctataaaaaa taccatgatt
       61 aatacatgga ataacattaa ggactctttc tggaatattg ttactggaat tgttaattct
      121 gctgaaaatg catggacaaa tctaaaaaat ggtgtttcaa atgctattaa tcgggtaaaa
      181 gaaatcttta attcgttaag ggaagtcaat ttatttgaaa ttggcaagaa catcattgat
      241 ggacttataa atggtgtaaa agaaaagtgg aatgctttga aagatactat caaaggtatt
      301 gcaggtaata tcaaaaattg gattaaagga gctttagata ttcattctcc atcaagatgg
      361 atgcgtgata tggttggtaa aaatattgtt caaggtatta tcgttggtat tgataaagag
      421 caaagtaagt tagatcaaac catgacaaat ttagtaaaaa caccgtctgt tcaacctgta
      481 gtaaatggaa taagtcacct accaattaca caaacaaagc aaaatatatc tgctagtgaa
      541 acaaaagaaa ttcatttaca tttaaatgtt tatggagatt tacctgattc aatggttcga
      601 aagattacta caaaaattaa aacagaattg acaagacaaa tgaaacgaga tgctggtgca
      661 gtaggaggaa cattatatgc aacttaagag aggacaattt tttattaatc aacattattc
      721 ttctgaattt aatgtgtata ttcaaaacag gcctgcttct gtttcagcta gtcgagtaat
      781 tgaattgaga gagcgagaag gaaatgattc tatcattatt gataaaggct attacaaaaa
      841 tgtgactaga aagattgagt gttactataa agcaccttca attgatatgg tgcaggagtg
      901 ggaagatcga atcactgaat ggttagatat gtcgtcttat agtgatttta ttctttacta
      961 tgatgaacaa tatatttatc aagctgtagt aatagaagga cctgaattta aaggaacaag
     1021 aaaaacagga aatattgtcc cttttgaatt tacagtgagt attcgaccat ttaaggaaaa
     1081 ttacaatggt agatttacta ttcgacaaac agaaactttt gaaatttata acccagagaa
     1141 gtattcttca aaaccgctta ttaaattgag tggttctgga gatgcttctt tttatattaa
     1201 taaggataaa tatgatttga aatcgttgga cagagaatta tttatagatt ctaaattaga
     1261 agaggcttat cgaaaattag atggtaattt agaacatcag gatcaggtca ccttattttt
     1321 agattttcca tttttatatc ctggaaaaaa tgaaattaaa tggacgaaaa atattcattc
     1381 atttgaaata atgcctaggt ggtggagaaa agtatgaaac caagaatata tagtcctact
     1441 gaaacagatt tctcaacgaa tggcttagga attttaaagg attgtacaag atgtgaaata
     1501 tatgaagtag ctaatggaaa atatgaatta gagttggatt atcctttagg aactagattt
     1561 gatgaatatt ttgaaaatga ctatcaaata aaagcaaagc caaacgatca agaagagtat
     1621 catatctttt ttattgatga taaagatata gatacttttt taaatacagt aactatttat
     1681 gctcagagtc gtacaaatcg acttggaaga cgggtagtca ctcttgtgga agtggactct
     1741 aaaactggtc aagaagcgat atcaattatt gaaactaaaa tggataaaaa atctgacata
     1801 cgactttatt ctgatattac ggctgtttct agcacaatct ttgaagcgag aaatgtttta
     1861 aattgtattg ctggtgaaca aggatcattg cttcaatatt ggggcggaga aattaaacgt
     1921 gaaccattta agctttcttt gttaaagcga agaggtcgca ataatgttgg aacgattcgg
     1981 tatggaaaag atatgtctgg tttgaaggtc aaattagatt ggacaggtat aaaaacaaga
     2041 attattccat atgctgatcc tcaaagtgaa gtaggtacga ctaatcgaat ttatggttcg
     2101 ccagtagata gtgcctatat taataactat cctgatgtct atacagaaca tgttcagttt
     2161 acagaagaac aaggagtaaa agacattaag agcttaaata aaatagctaa aaattacttt
     2221 aaaacgatca atcctggctg tgataagcct aaagtttcta ttacggttga atttgataag
     2281 ttgactgata gtgaagaagc gaaagaattt gcgaagatta gaaattatgg tctgtttgat
     2341 acatttaaaa tatatcataa aaaatataat atttatcttg aatcaaaggt tagtggacta
     2401 caatacgatt ctttatctga aaaaactttg aaattagaag caggagacat tcaagttgct
     2461 ttttatcagc aacaagctgt aactattcag gataaattaa aagattatgc aacgaataat
     2521 tatatgagtg attttaatga ttatgtttct tcaatgatta caggtcaagg cagtgcagga
     2581 ggatatgtag ttttatggcc aaaagaaaaa ccttccaaca tctttattat ggataatcca
     2641 gatttagaaa aggcaagaga agtactaaga atgaataaaa atgggattgc tttttctaag
     2701 aaaggatggc gggggccttt taattcagct tggacattag atagtatatt taatgccaat
     2761 tttatacaaa ctggattaat caaagcagat atctttcaaa attcttttaa caagactgga
     2821 gatgtattga aattagttaa tggactactc caaatttgga acaacaaaaa gaaaataatg
     2881 gagttaacta aaaaaggaat ggaattttgg aattctaagg gttcaattgg aacgattgga
     2941 acaactgatt ctgttggtaa tccttttcct ggggcttcta ctccaacccc tattgaagat
     3001 aattctttag ttattcgtac aaatggagac ggcaaatata ttttgatttc tcctaaagta
     3061 ggaaaaggat tagttttatt aggaaatggt aaagcaattt attttggaga cttagatgta
     3121 caaggaaaac ttacagttaa aggaaaagaa ataattcctg gacaaggtgg tggtccatcg
     3181 ggaggtggag aatctactgg tgggtatcct agtgaattaa aaacagatgc agaaaagaga
     3241 gcttggagaa tttacgatat tttgtgtaat aacggattta cgaaacaatc tgcatgtgga
     3301 atattgggga acattcaaca agaaacagga ggaacttttg atcctgatac tgttcaaata
     3361 ggtggaccag catacggatt agttcagtgg gatggttctt catatcctct tgttggtcca
     3421 gcaacatggg atggaaaagt ttatgttcaa aacttattta atgctgcagg tattaaagaa
     3481 ccaataacga gcttagatgc acaagttcga ttgcttattt ggacatttac aaatggacaa
     3541 tggatgggcg tagtacaacc tacgacggtt gatggattta aggcctgtac tgatccaaga
     3601 caagcagcat atgcttttga acgaaactat gaacgtccgg cagcgacaca tcctgaacgt
     3661 caagattatg cagttaactg gtataacaaa tttaaagatt taaaaccagg aggagctact
     3721 ggagaagcag gactaaaaca tttagagtct ttgattggac aacgaattgg caatggtcag
     3781 tgttatggct tgtctgcaga atattcagga tatctgggtg gttgtggcat gggtgctgga
     3841 acaaaatatg gtttaactca tgtgatagga aatacttctg cagcatctga tattggtatt
     3901 gcctatgatt ggtctgctgt tggttggaaa gtgattcaaa accctagata tgatcaatta
     3961 gtagttggtg caattattaa ttgggcaaga ggtggacaag tgggttcatg gtttgcagat
     4021 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
     4081 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgacagta ttatagtgca
     4141 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
     4201 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
     4261 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
     4321 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
     4381 gaaacaaaaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
     4441 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
     4501 ggaacaactc aagacttctc ttattttgtc atcaatgctg tttcaaaaac agaaggagaa
     4561 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
     4621 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
     4681 agagaggaac aggagaatag tttccaagat tggagagaag gacaagaatc agattacttg
     4741 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
     4801 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
     4861 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
     4921 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
     4981 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     5041 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     5101 ttaaagtaaa gagaatgatg gaaactgatg aatctggtgt taaacgtcaa tttatgccaa
     5161 ttacacatgt ctctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     5221 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     5281 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     5341 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     5401 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     5461 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaaaggaaaa ttagaatctc
     5521 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     5581 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     5641 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     5701 ttggggaggt atagagaatg acagatatcg taaaaataaa acaaagcaat gtacaggttt
     5761 atccacaaac tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag
     5821 gcgatcctgg ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt
     5881 ctgttacgaa tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag
     5941 ataaaggtga tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg
     6001 ggttgatgtc gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa
     6061 atccaggtaa tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac
     6121 ttgatggatt agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt
     6181 tcaattaaaa gaagacggag ttgctaaata cttaaaaacg catgtagaag ctattgatgg
     6241 taaagaagca ttggtgcaga cagatggaga ccaagccatt gcaggacata agaatttttc
     6301 aggttctgta acaatcaata ataaacgtgt tttgacgact gatgataaca gatatgaagt
     6361 ggtaaatctg gttgtcacta acggtaatac agggacagca aagctttatc gtgaaggaaa
     6421 aacagtttct atatattttg tggctttaaa cggaaaaagt agtggcggga atgattcggt
     6481 tattttaact gttccagaag gctatcggac accaattagt tttgaacaac tggttgggtc
     6541 aatagaccgt tctactttga acagtgctca actatctatt ggcgcagacg gagccattaa
     6601 atggagaaga aattcaagtt atggatcagc ttattcattt gttatcactt attcaattta
     6661 agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa gccttacgga ttcgagcaaa
     6721 tcattttgaa caatcaagaa aatatccctg aaaactgtac agagattaaa ccaccagttc
     6781 ctaactggag accaagattt gattttgata aaaaacagtg ggttgagtta gctactgaag
     6841 aagagaaaaa agggaatgct gttgatagcg tagatgaatt ggctaatatt aaggcattat
     6901 acgaaacgct gaaggcagaa aatgatgaat tgaaacagct aaattctaaa gcaatgctaa
     6961 acaatgtagc aatcaagcaa gaaaatgtct tattgaaaga aaagtcagaa agtttagcgc
     7021 agttgaattc aaaaacgatg cttgcttctg tacaaaatac caaggaaatc gaagaaatca
     7081 aaaaacaatt acaaggtggg aagtaacatg tattcatatg aagatatcaa actaatgtat
     7141 gactggggct gtttcactaa cgaacaagta atggttttcg ttccattatg cattacagac
     7201 gaagaagcag ataaaatcat taataaagat aagagcgcat cttaattgat gcgtttttat
     7261 ttaaggtaaa ggagttgtca catgattaat ttaggggaat ggggaatgat agcaggatca
     7321 ataaccgcta tcgtttcttt gattttatta gtaattagac cgattgctgc atctttctcg
     7381 aaaattactg agactctttc aaaagtaagc cacaatttag atttgctgac taaagattta
     7441 gaatcgagca aatcagatcg attgatgatt catgaagaac taaagaaaca cgatgaaaga
     7501 ttagatacac atacagaaaa attggtggaa cacacgcaac aaatcaaaac tttgtttagg
     7561 gagagaagaa aatgaataat aaaacgttcg aagtactaaa atggttcgca ctggtaatta
     7621 ttcccgcact agctactttc gtggggttag ttggtaaagc gctcaattgg cagtacacag
     7681 atatctgtgt tgtcatcatt actggttttg gcgcgttttt agggagtgtg ttgggtgtat
     7741 caaatcgaac ctacaaaatg ttctcggctg aaagcgaaga aggaggaaac aaatgaaaaa
     7801 gaaaattact attactgcga tgagcctgtt aacggctctt tttttattac ccattaatac
     7861 gtttgcttat actattaatg acgagtataa tttagcgccg aatcaaggag actccagatt
     7921 agcaattcct aacaaaatta ttttgcacga aactggaata gatgcaccag caagaaacgt
     7981 agccgccaac atgaaaaata attataacgg aagcaattct tatactacag atgttattgg
     8041 tgacggtggg attgtttacc gtgtgggtga gcaaggatat gtttcgtggg gagctggtaa
     8101 cgctaatcct tatgcgcctg tacagattga attacagcgc acatatgata aagcattgtt
     8161 tgaaaaaaac tatcgagctt atattgaata tacaagagat agtgcaaaaa aatatggaat
     8221 tccattgact cttgatcaag gaacttcttt atttacaaaa ggaatcattt ctcatttgtg
     8281 ggtgacaaat tatgtttggg ggaaccacac agatccatat ggttacttat cgcaaatggg
     8341 agttagcaaa gaaaagcttg cttatgattt agctcatgga tttaccgatg aaaatccaac
     8401 aacttctgaa aacaagcctg tcattgatcc aacacgagct ggtgcagcta atcctacgct
     8461 gacagatgga acaaatcacg cccacattga tcagtttggg gaaatcgaaa acgcaaactt
     8521 gcatgtggct ggatggcaca ttgctaacta taaatacgag tatattttca ttatggacta
     8581 caatactggg aaagaattag ctcgagtaag agctgatgga atttatagac cagatgtaaa
     8641 ccaagcttat aatacttctg gaaatgttgg ttatcatgta tctttcaata tgcgtaattt
     8701 tcctagtaag aaagtctatg taatgatgcg ggcaacgaat gatccagagg gaaacactaa
     8761 aggcggtgcg caagatttcc atgacaaacg ttggtattta aatattccta aacgataaaa
     8821 atagctcctc gttgaggagc agtacataac tatattgaca actataaaaa ttattcgata
     8881 aaatagtgat gttatcgcat atcttcacta tcacccataa atagtcacac tccaagctat
     8941 gcgataacag gtttgttgcc acacattcta ctggttgatt gtttatggct ttatgtggca
     9001 acaaccagta cccttagctc agttggtcag agcagacggc tcataaccgt ccggtcgtag
     9061 gttcgagtcc tacagggtac attaacgtag ccatttgaat cgttctgtgt tagaattttt
     9121 ttgaagagta ttatacaagc taaagctttt cttcattgcc actcaaatga gtggcttttt
     9181 tatgtatcct tttatggatt aatgaaagga tgtttcacat agttatactt ctgtatattt
     9241 gaaaagtttt actttgattt ttaaatagaa agacat
//
LOCUS       DAYYAJ010000116         9237 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000116 DAYYAJ010000000
VERSION     DAYYAJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.116"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..659
                     /locus_tag="ACHATZ_002404"
     CDS             12..659
                     /locus_tag="ACHATZ_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8404882.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            743..1687
                     /locus_tag="ACHATZ_002405"
     CDS             743..1687
                     /locus_tag="ACHATZ_002405"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8404883.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1822..3273
                     /locus_tag="ACHATZ_002406"
     CDS             1822..3273
                     /locus_tag="ACHATZ_002406"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8404884.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3294..4130
                     /locus_tag="ACHATZ_002407"
     CDS             3294..4130
                     /locus_tag="ACHATZ_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8404885.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4130..4789
                     /locus_tag="ACHATZ_002408"
     CDS             4130..4789
                     /locus_tag="ACHATZ_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8404886.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4915..5604
                     /locus_tag="ACHATZ_002409"
     CDS             4915..5604
                     /locus_tag="ACHATZ_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404887.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5610..6566
                     /locus_tag="ACHATZ_002410"
     CDS             5610..6566
                     /locus_tag="ACHATZ_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8404888.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6610..7122
                     /locus_tag="ACHATZ_002411"
     CDS             6610..7122
                     /locus_tag="ACHATZ_002411"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404889.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7378..7926)
                     /locus_tag="ACHATZ_002412"
     CDS             complement(7378..7926)
                     /locus_tag="ACHATZ_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8404890.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8015..9196)
                     /locus_tag="ACHATZ_002413"
     CDS             complement(8015..9196)
                     /locus_tag="ACHATZ_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8404891.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         3056 a         1698 c         1867 g         2616 t
ORIGIN      
        1 ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag
       61 ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac
      121 agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga
      181 caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga
      241 tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg
      301 attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa
      361 gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg
      421 aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg
      481 aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt
      541 tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta
      601 catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag
      661 aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa
      721 cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc
      781 caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact
      841 ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg
      901 aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc
      961 aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa
     1021 ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat
     1081 gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc
     1141 caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa
     1201 agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt
     1261 tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc
     1321 tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa
     1381 accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa
     1441 tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat
     1501 tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg
     1561 taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca
     1621 gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat
     1681 ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg
     1741 tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac
     1801 agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa
     1861 aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat
     1921 catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg
     1981 attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt
     2041 caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct
     2101 tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa
     2161 accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca
     2221 agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca
     2281 gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata
     2341 cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt
     2401 ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg
     2461 agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa
     2521 gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt
     2581 gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct
     2641 gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt
     2701 cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc
     2761 tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg
     2821 actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta
     2881 tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca
     2941 ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga
     3001 gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc
     3061 atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa
     3121 ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt
     3181 atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat
     3241 catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga
     3301 aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag
     3361 ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag
     3421 tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg
     3481 gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta
     3541 aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg
     3601 cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca
     3661 aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg
     3721 aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg
     3781 aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg
     3841 atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg
     3901 tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg
     3961 aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat
     4021 taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg
     4081 atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc
     4141 tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata
     4201 cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt tgcttttgac
     4261 agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt
     4321 ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt taccttatca
     4381 attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg
     4441 tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc
     4501 ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat
     4561 ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc
     4621 aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga
     4681 ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga
     4741 aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa aaatagaaat
     4801 ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt
     4861 ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag
     4921 accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc
     4981 ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc
     5041 ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca
     5101 gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca
     5161 agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat
     5221 cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa
     5281 gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct
     5341 ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaca
     5401 gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa
     5461 cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa
     5521 gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg
     5581 ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat
     5641 ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac
     5701 cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa
     5761 aaaagcacca tcgaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg
     5821 atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct
     5881 taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg
     5941 gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat
     6001 ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc
     6061 gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt
     6121 ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga
     6181 tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa
     6241 cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact
     6301 ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca
     6361 tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc
     6421 agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg
     6481 aacttattag tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc
     6541 aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga
     6601 ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc
     6661 attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag
     6721 aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata
     6781 gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc cgtttacaca
     6841 aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct
     6901 ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg
     6961 attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg
     7021 atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt
     7081 gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc
     7141 aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa
     7201 cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg
     7261 tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc
     7321 gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc gatacattta
     7381 tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac
     7441 gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac
     7501 cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa
     7561 tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa
     7621 atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt
     7681 aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc
     7741 atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc
     7801 tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa
     7861 aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata
     7921 attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc
     7981 aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca gtcacaatag
     8041 cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa
     8101 gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga
     8161 agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga
     8221 aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca
     8281 tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg
     8341 caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat
     8401 tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat
     8461 ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt
     8521 caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa
     8581 tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg
     8641 ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga
     8701 acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt
     8761 gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt
     8821 tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa
     8881 taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac
     8941 ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa
     9001 gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata
     9061 atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg
     9121 cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac
     9181 ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt acctgtt
//
LOCUS       DAYYAJ010000117         8942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000117 DAYYAJ010000000
VERSION     DAYYAJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.117"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACHATZ_002414"
     CDS             complement(15..1154)
                     /locus_tag="ACHATZ_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8404892.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACHATZ_002415"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACHATZ_002415"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8404893.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACHATZ_002416"
     CDS             complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACHATZ_002416"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8404894.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACHATZ_002417"
     CDS             complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACHATZ_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8404895.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACHATZ_002418"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACHATZ_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8404896.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="ACHATZ_002419"
     CDS             complement(6168..7259)
                     /locus_tag="ACHATZ_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8404897.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACHATZ_002420"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACHATZ_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8404898.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="ACHATZ_002421"
     tRNA            8802..8873
                     /locus_tag="ACHATZ_002421"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3081 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aa
//
LOCUS       DAYYAJ010000118         8784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000118 DAYYAJ010000000
VERSION     DAYYAJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.118"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHATZ_002422"
     CDS             complement(<1..244)
                     /locus_tag="ACHATZ_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8404899.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACHATZ_002423"
     CDS             complement(237..1829)
                     /locus_tag="ACHATZ_002423"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8404900.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="ACHATZ_002424"
     CDS             complement(2339..3064)
                     /locus_tag="ACHATZ_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404901.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="ACHATZ_002425"
     CDS             complement(3218..3544)
                     /locus_tag="ACHATZ_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404902.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="ACHATZ_002426"
     CDS             complement(3581..3946)
                     /locus_tag="ACHATZ_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8404903.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="ACHATZ_002427"
     CDS             complement(3983..4198)
                     /locus_tag="ACHATZ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8404904.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="ACHATZ_002428"
     CDS             4327..4662
                     /locus_tag="ACHATZ_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8404905.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="ACHATZ_002429"
     CDS             complement(4738..6735)
                     /locus_tag="ACHATZ_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404906.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="ACHATZ_002430"
     CDS             complement(6833..7108)
                     /locus_tag="ACHATZ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404907.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="ACHATZ_002431"
     CDS             7340..8074
                     /locus_tag="ACHATZ_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8404908.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..>8784
                     /locus_tag="ACHATZ_002432"
     CDS             8113..>8784
                     /locus_tag="ACHATZ_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8404909.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNK"
BASE COUNT         2846 a         1664 c         1266 g         3008 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaa
//
LOCUS       DAYYAJ010000119         8764 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000119 DAYYAJ010000000
VERSION     DAYYAJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.119"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..2632
                     /gene="adhE"
                     /locus_tag="ACHATZ_002433"
                     /gene_synonym="adhC"
     CDS             35..2632
                     /gene="adhE"
                     /locus_tag="ACHATZ_002433"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8404910.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2861..3967
                     /locus_tag="ACHATZ_002434"
     CDS             2861..3967
                     /locus_tag="ACHATZ_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8404911.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3984..5012
                     /locus_tag="ACHATZ_002435"
     CDS             3984..5012
                     /locus_tag="ACHATZ_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8404912.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5018..5497
                     /locus_tag="ACHATZ_002436"
     CDS             5018..5497
                     /locus_tag="ACHATZ_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8404913.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5513..6808
                     /locus_tag="ACHATZ_002437"
     CDS             5513..6808
                     /locus_tag="ACHATZ_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8404914.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6819..7643
                     /locus_tag="ACHATZ_002438"
     CDS             6819..7643
                     /locus_tag="ACHATZ_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8404915.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7808..8707
                     /locus_tag="ACHATZ_002439"
     CDS             7808..8707
                     /locus_tag="ACHATZ_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404916.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2796 a         1531 c         1956 g         2481 t
ORIGIN      
        1 taacaaataa ccatgaccca ttaggaggat aatgatggct aaagtaatag aaaaagaaaa
       61 aacgaagaca attgacgtac aagcaatgat tgatgatttg gcagaaaaag caaatgcagc
      121 attgaaagaa atggaaagct ttgatcaaga aaaagtagac catatcgtgc atgaaatggc
      181 aatggcagct ttggatcaac atatgccatt agcgaagatg gctgtagagg aaacaggccg
      241 cggtgtttac gaagataaag caatcaaaaa tatgtatgcg tccgaatata tttggcataa
      301 tatcaaacat gataaaacag ttggtgtgat caatgaagat gtccaaaaag ggttgattga
      361 aattgctgaa ccagtcggtg tggtttgcgg agtaactcca acaacgaatc cgacttccac
      421 aacgattttc aaatcaatga tcgcgttgaa aacaagaaat ccaatcgttt ttgctttcca
      481 tccaagtgcc caaaaatctt ctgcagaagc agcacgagtg gtacgtgatg ctgctatcgc
      541 tgccggagct cctgaaaatt gtatccaatg gatcgaacat ccttcgatcg aagcgacttc
      601 tatgctaatg aaccatccag gtatcgcgat cgtcttagcg acaggtggag ctggaatggt
      661 caagtctgct tactctacag gaaaaccagc tttaggtgta ggtccaggta acgtaccagc
      721 ttatattgaa aaaacagcaa aaattaaacg tgcagtgaac gatttgatcg tttcgaaaac
      781 atttgataat ggaatgattt gtgcttctga acaagctgtg atcgtggata aagaaatcta
      841 tgcagcagta aaagcagaat tccaagcaca ccaagtttat atcgtaaaac cagatgaatt
      901 gcaaaaatta gaagatgcag tgatgaacga aggaaaatat gcggtcaatc catcaatcgt
      961 tggacattct gcgatggaaa tcgcaaaatt ggcaggaatc agtgtaccaa aaggaacgaa
     1021 aatgctgatt gctgaattgg aaggcgtagg cccagattat ccgctatcaa gagaaaaact
     1081 ttctccagtt cttgccatga tcaaagctaa caacacggac catgcgtttg atttgtgtga
     1141 aggtatgttg aatcttggag gattaggaca tacagcagtt atccattcag aaaacgaaga
     1201 gttgcatgtc aaattcggtc ttcgaatgaa agcttgtcgt attttagtga atacaccatc
     1261 tgcagaaggc ggcatcggtg atatctataa cgaaatgatt ccgtcattga ctttgggatg
     1321 cggttcttat ggtaagaact ctgtttctcg taacgtctct gctgtaaact taatcaatgt
     1381 caaaactgta gcgaaacgga gaaataacat gcaatggttc aaattaccgc caaaaatctt
     1441 ctttgaaaag aactctctat tgtacttaga gaaaatggaa aacgttgaac gtgtcatgat
     1501 cgtttgtgac cctggtatgg tccaattcgg ttactgcgat acagttcgtg aagtattggc
     1561 acgacgtaaa aatgatgtga agatcgaagt cttctcagat gttgaaccga acccatcgac
     1621 aaataccgta tacgcaggaa cgaaactgat ggcagacttc aagccagata cagtaatcgc
     1681 tcttggaggc ggttctgcaa tggacgcagc taagggaatg tggatgttct atgaacaccc
     1741 agacacatct ttctttggtg caaaacaaaa attcttggat atccgtaaac ggacatataa
     1801 gatcgacaaa ccagaaaaaa cacaattcgt atgtatcccg acaacatcag ggactggttc
     1861 tgaagtaacg ccatttgcgg tcatcactga cagtgaaaca catgtgaaat atccgttagc
     1921 agattatgca ttgacgccag atgtcgcaat cgtcgatcct caattcgtca tgtcagttcc
     1981 agcatctgtc acagcagata ccgggatgga tgtgttaaca catgcaatcg aatcctatgt
     2041 ttcagtcatg gcttcagatt atacacgtgg attgagccta caagcaatca agttagtctt
     2101 tgactattta gaaaaatcag taaaaacacc tgatatggaa tcacgtgaaa aaatgcacaa
     2161 cgcttctaca atggctggta tggcgtttgc taacgcattc ctaggtattt gtcactcagt
     2221 cgcacataaa atcggcgggg aatacgggat ccctcatgga cggacaaatg cgattctttt
     2281 accgcatatt atccgctata atgcaaaaga tccacaaaaa catgcgatgt tccctaaata
     2341 tgactatttc cgtgcagata cagattacgc agatatcgca aaattcttag gtctgaaagg
     2401 cgaaacaaca gaggaattag tcgaagcttt agcaacggct gtttatgaat taggaaaatc
     2461 tgtcggcatc aacatgagtc taaaagctca aggagtcaca caagaaacac tagatacaac
     2521 cgtagaccgt atggctgaat tagcttacga agaccaatgt acgacagcta atccgaaaga
     2581 accattgatc tctgaattga aacagatcat cattgatgct tacaacggat aagcaaaaga
     2641 aagcgtagaa aaaagataag aaaatagatg aaaaagaact tggaaaacgt tgtttctcca
     2701 agttcttttt ttaatttcca agtaactgaa agttactttt gttatttaac atttaggtca
     2761 attttagaca gtttttgttc aaaaagcgat atttatagtg gacggtatta ctgctatatt
     2821 aaggatataa aatgaaagcg tttaaaaagg tggcgtaaag atgattatta aagaagagat
     2881 ttttgtacct aaagaattag tgaaagaaaa aattgacctg ctcgttgaaa acctgacaac
     2941 gatcaaagac gataatggtg aatttttgtt ggactttgat ggactgaaag tagatgacaa
     3001 aagctggaca gtgtggaatt ggccacaagg tgtcggtcta tatggtattt ataaaaatta
     3061 ccagatgaca aaaaatcctc gcgcttatca agtggtcaat gaatggtttg aggatcgtat
     3121 ggaagaaggc gctccgccca aaaacgtcaa tacaatggca ccgctattga ctatggctta
     3181 cttgtatgaa gacacgaaag attcacgcta tttgccttat ttagaacaat gggcagaatg
     3241 ggtcatgaat gacatgcctc ggacaaatga agacgggtta cagcatgcca catatggacc
     3301 ggaaaataag aatcagttgt gggacgatac attgatgatg acagtgcttc ctttggcaaa
     3361 aatcgggaaa ctgttgaatc gcccagaata tttagaagaa gctcgttacc agtttatgat
     3421 ccatatcaaa tacttgatgg ataaacgaac aggattgtgg tatcacggct ggacgtttga
     3481 aggaaatcat aactatgccg aagcattttg ggctcgtgga aactgctggt tgacgattgc
     3541 tattccagag atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt
     3601 gatcgaaaca cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt
     3661 atggcatacg ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt
     3721 tgcttacggt attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt
     3781 cgcttatcgt gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa
     3841 cgtttcaatc gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac
     3901 agcaatgcct tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta
     3961 ttgttaaaag aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc
     4021 ttagcctagg tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg
     4081 cagatgcaga tgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagc
     4141 cagtaattga tgcaatgaat gaatttgggc gtttagtaaa agaaaaaaca gatggttccg
     4201 tacagatcca gtatttccca gatggacaac tgggtggaga acgtgaactg atcgaattga
     4261 cacagtcagg agcagtagat ttcacaaaag taagtgcctc agcgttggaa agtttctcta
     4321 aagattattc gattttttca gttccttatc tattcaatga tgaagaacat ttctttaagg
     4381 tgatggataa tgaagaaata atggagccaa tctaccaatc gaccaaaaat ttaggattta
     4441 ccggattgac ctattatgat tctggtcagc gcagctttta tatggtagac ggtccgatcc
     4501 atacgccgga agatttgaag ggaaagaaga tccgtgtgat gcaaagcgaa accgctatca
     4561 aaatggtaga gcttttaggc ggttcgcccg tacctatggg aagtgatgaa gtgtacacgt
     4621 ctcttcaatc caatttgatc aacggatcag aaaacaatga atttgtcttg tatacagctg
     4681 gacatggtgg tgtggctaaa tactattcct atgatgagca tacacgtgtg ccagatatca
     4741 tcatcatgaa tgatgccatc aaagaacagc taactgatga gcaggaaaaa gccattgaag
     4801 aagcagcgaa agaatcaacg gtttttgaaa ttgaagcttt tgcaaaagca attgaagaag
     4861 aaaaagcttt agcacaagaa gaacatggag tccaattcaa tgatgtagat aatacaccgt
     4921 ttcgagaggc agttgcccca cttcatgata tattcaaaaa cgatcctacc tacaaagacc
     4981 tttatcaaaa gatccaagag caaggagcgt agctgtgatg aaaatgattt taaacaaggt
     5041 attagagatc ctgggagctg ttttgcttgt tgggatggtc gctgttgttt tatggcaagt
     5101 attttccaga acggttttag gaaatccaaa tacggtcacg gaagagcttg ttcgattcgg
     5161 tcttgtctgg tttgccatgt tgtcttccgc gtatgttgtg ggacaaaaag cacatttagc
     5221 cgtaacggtt ttaagtgaaa aattaactgg caaaaaaagt aaaattttag aagttgttgt
     5281 tcaaagttta tttctggtat ttgctgcgat gatcatggtg tatggcggat ggaatgctgt
     5341 cacattgaca atggggcaga tctcgccttc tttaagtatt cctatgggat atgtttatct
     5401 gtcagtacct gtatcggggg tactcatcat tatctatagc ctgatcaatc taatcgatac
     5461 attaagcaac aagccagcaa ttcaagaaaa aatataaaaa tggaggaata aaatggcaat
     5521 agaagccgga ttagtgttat tttttgtatt tgttgtgttg cttgtattag ggatgccgat
     5581 tgctataagt attgcattct catcgatgat gactttattg ttagttatac cgtttgatgt
     5641 ttctgctttt agttcagctc aaaaaatggt gggaagtatc aatagttttt cgctgatcgc
     5701 tattccgttt ttcgttctct cgggcattat catgaataat ggagggatag ctaagaaact
     5761 agtggatttt gcgatgttgt tcgtcgggcg cgtgccgggg gctttggctc atacgaatgt
     5821 gttagggaat gctttatttg gttccatctc tagctcggcc attgccgctt ctaccgcgat
     5881 tggcggagtg ttgatccctc agcaagtgga agaaggatat gaccggaagt ttgcgacagc
     5941 ggtcaatatc gcgtcagctc cgacaggtat ggtcattcct ccaagtactg cttttatcat
     6001 gtattcactt gttgctggtg gtgcttcgat ctctgctttg tttatgggag gttatctcgt
     6061 aggcgctttg tgggcgctag gaatcatgct agtagcattc atcgttgcta aaaagaataa
     6121 ataccctgtt gtcgaaaaag gacagatggc tcatgctgga aaaataataa aagaggcgat
     6181 tcctagcctt ctattgattg tcatcattat cggtggtatc ttgacgggga tatttacagc
     6241 agtagaagct tcagcgatag ctgtcattta ctccctgttg attgctatgt tttactataa
     6301 aacggtaaaa ctaaaagata tacctaatat gttgtttcaa tcagtagcaa tgtcagggac
     6361 gatcatgttc ttattggcta cttcttccat gatgtctttc gccatggcgt ttaccggtat
     6421 tcctcaggca attagttcgt tgattatggg cgtaacggat aataaattta ttattctgtt
     6481 attagtcaat cttgtgttat tactggtggg tatgtttatg gatgtggcgc ctgctatctt
     6541 gatttttaca ccaatatttt taccaatcgt cacaagtgta ggggttgatc ctgtacatta
     6601 cgggttgttt tcaatcatga atctgtgtgt aggctccatc acgccgccag taggtacagg
     6661 actatatgtc ggtgcaagtg taggaggcgt aaaagcagag gcaatgctga aaccgcttct
     6721 tcctttttat ggggttatct tagtcgtctt gtttttgatt acttacttcc cgcaaatcgt
     6781 catgtggcta cccaatatgc tgagttagga gaatagttat gttgaacatt acaattagag
     6841 gacatgatct ttccgaagtc caaacgatag aagatcttgc agaaaaaaca aaagaacaag
     6901 ggattcatac actgcagctc gccttaggtc tttcgtttcc tgaaatacct tctggcgcaa
     6961 atgagatcaa ttctgggatg ggaaattatg tcaaacgagt tttagaaaag caagaagtga
     7021 ccgtaggaat tttaagttgc tatatcaata tgattcatcc agatttaacg atacgacaag
     7081 agttgctgac aaagttcgag cagtatcttc gctatgcttc ttcctttggt gcgtcgatgg
     7141 ttgcttcgga aactggatgt gttttgccgg aaatccaata tacggaagaa aatttcacag
     7201 atgaagcatt tgctgaagct gtgtccgtta tccgtcgctt ggtaaaagct ggagaaaaat
     7261 accaaatgat ggtggggata gaaccaggtt tgaatcatcc ggtgtattct ttagcaaggg
     7321 tagagcagct gatccaagca gtagattcgg actatctagg aatcatttta gatccgacca
     7381 atttgatcac aagtagtgat tatcaagaac aagtccaatt ggtagaagaa gcttttgaac
     7441 gttttggtga aaagatatgt gcggttcatt tgaaagattt tcgtgtcgaa caggaaaaaa
     7501 tcgtgccggt aaatttggga gacggtgtga tcgagtacac aaaaatcaaa gaaatcatca
     7561 tgaagaatcg tccgtacctg tatgtggttc ttgaagaaac aaaagatgat gggattcgtt
     7621 atggaagaag tcttcttgaa taatgtaaag aagacgttgg ttaactaata tttagaatgg
     7681 agggggcggg gctagaagat gccacgtctc ttctttttta taagagtata taaaagcagc
     7741 tctttttgat tacaaaaaac gtggtataat aaaattgtgc ataagtgaac aaaaggaggt
     7801 gcggcacatg ctccaaaaga aaatcagcgg tgaacaagtc aatacgatgg tttctgatca
     7861 gtatgaaatc tatcatgtca aagatgtcgt cagccaaaat aaaacgatct atcatcatca
     7921 tgatttctat gagatccatg ctacattaaa gggagaagcg actttttatt tgaacggacg
     7981 acaatttacg atcaaagcag gaagtgtgct gctcatccat tccaatgatt tgcatcgtat
     8041 cgtccgccaa tcttcagatg tattcgagcg tgtctatatc tttgtcaccc cttcttttct
     8101 agaaagtcgt tccacgccat ggtctaatct atcggcttgt tttcaaccaa tgggagagaa
     8161 gaggagtcgt gtattgcaaa tagaacagga agtattgaag caaaaattat catttatcga
     8221 ccatcctcct gataaaaaaa tttacggtga agacataaaa tatgaacaag ctctggtaga
     8281 atacttgatt tttctcaatc aagttgtaca aaaagaagag agtgcgcaag aacagaatgc
     8341 tgtagttcct aatgagcgtg tggaacaaat gttacaatat atctcaaaaa atttatctca
     8401 tccgctttct ttagaggaaa tggaaaagca gttttttgtc agcaaatacc atatcacacg
     8461 agaatttaaa aagtacactg gatttacgtt tcatcagtat gtaatgaaga aaaagctgat
     8521 ctatgccaag cacttgttgc gagaataccg cagctctagt gctgtatata gtaaaagtgg
     8581 tttttcttct tatccgcatt ttctgaaagc attcaagaga gagttcggca taacgcctaa
     8641 agaatttttg aaacgtgata cacagaaaca gttggttcat tatgaacatt ttgaagatga
     8701 acaatgagaa tgatcactaa aaaataaccg cagtagctgc tgttgtttac atagaagcag
     8761 aaac
//
LOCUS       DAYYAJ010000120         8675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000120 DAYYAJ010000000
VERSION     DAYYAJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.120"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHATZ_002440"
     CDS             <1..1101
                     /locus_tag="ACHATZ_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8404917.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHATZ_002441"
     CDS             complement(1280..1615)
                     /locus_tag="ACHATZ_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8404918.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHATZ_002442"
     CDS             complement(1599..1862)
                     /locus_tag="ACHATZ_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8404919.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHATZ_002443"
     CDS             complement(2322..3125)
                     /locus_tag="ACHATZ_002443"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8404920.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHATZ_002444"
     CDS             complement(3103..4227)
                     /locus_tag="ACHATZ_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8404921.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHATZ_002445"
     CDS             complement(4243..5058)
                     /locus_tag="ACHATZ_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404922.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHATZ_002446"
     CDS             complement(5048..5920)
                     /locus_tag="ACHATZ_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8404923.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHATZ_002447"
     CDS             complement(6005..7294)
                     /locus_tag="ACHATZ_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8404924.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHATZ_002448"
     CDS             complement(7438..7950)
                     /locus_tag="ACHATZ_002448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404925.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHATZ_002449"
     CDS             complement(8017..8160)
                     /locus_tag="ACHATZ_002449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8404926.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAYYAJ010000121         8656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000121 DAYYAJ010000000
VERSION     DAYYAJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.121"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..328)
                     /locus_tag="ACHATZ_002450"
     CDS             complement(35..328)
                     /locus_tag="ACHATZ_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8404927.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(356..1021)
                     /locus_tag="ACHATZ_002451"
     CDS             complement(356..1021)
                     /locus_tag="ACHATZ_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8404928.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1161..1793)
                     /locus_tag="ACHATZ_002452"
     CDS             complement(1161..1793)
                     /locus_tag="ACHATZ_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8404929.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1800..2867)
                     /gene="liaS"
                     /locus_tag="ACHATZ_002453"
     CDS             complement(1800..2867)
                     /gene="liaS"
                     /locus_tag="ACHATZ_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8404930.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(2864..3595)
                     /gene="liaF"
                     /locus_tag="ACHATZ_002454"
     CDS             complement(2864..3595)
                     /gene="liaF"
                     /locus_tag="ACHATZ_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8404931.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(3763..4242)
                     /gene="greA"
                     /locus_tag="ACHATZ_002455"
     CDS             complement(3763..4242)
                     /gene="greA"
                     /locus_tag="ACHATZ_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8404932.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4378..5553)
                     /gene="mltG"
                     /locus_tag="ACHATZ_002456"
     CDS             complement(4378..5553)
                     /gene="mltG"
                     /locus_tag="ACHATZ_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8404933.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5718..6878
                     /locus_tag="ACHATZ_002457"
     CDS             5718..6878
                     /locus_tag="ACHATZ_002457"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8404934.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6918..7865)
                     /locus_tag="ACHATZ_002458"
     CDS             complement(6918..7865)
                     /locus_tag="ACHATZ_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8404935.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7898..>8656)
                     /locus_tag="ACHATZ_002459"
     CDS             complement(7898..>8656)
                     /locus_tag="ACHATZ_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8404936.1"
                     /translation="VLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSS
                     IGSVLILFALFNATKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLV
                     RKDVAENTDISNARITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAI
                     HNAYLNVLTSTGILGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALY
                     AALNNELVYENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         2608 a         1788 c         1341 g         2919 t
ORIGIN      
        1 gaaggtgaca aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg
       61 gctcctccag gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat
      121 ctgcttcctc gataaaaaaa agtgtcccat ccatttccac tttctttaca ggatgttctg
      181 gctgttcaac tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt
      241 ttccaaaaaa acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt
      301 gtgcttttgg cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt
      361 gattcatctt atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat
      421 cattttcacg aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc
      481 gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt
      541 tgaaaaattt aggattggag actcggattt ctgctaaaga atatttatct gacagctcta
      601 agtagtctag aatattcttt gatactaagt tatgtgcaat ccgtatcccc atgtctcgct
      661 cagggtgtac gacacgatct gctccgatct tctctagtac acgggcatgg tactcatttt
      721 gggcttttgc taaaatattc ggtacgccca tttcctttac catcagagta actaagatgc
      781 tggcttggat atcttctcca atagcgacaa ttacatgatc gaaatttctt atacctaatg
      841 aacgaagtgt catctcatcc tgtgcatttg ccacaacagc atgggtggct atattcatat
      901 attcattgac tcgatcttca ttactgtcaa tagccagtac ttcttgtcca gcttcgatca
      961 gtgtacggca gatgcttccg ccaaatcgtc caaggcctac aattgcaaaa ttttgtttca
     1021 ccattttcct cctttagtat cttcttttct cagtataaca aagcctttcc aaaaacaaaa
     1081 gcaagcatca ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat
     1141 tatcctcttt atcatcgttt ttatttcacc aaaccatgtt taaacgcata gatagctgct
     1201 tgagttcggt cttctacctc taacttagct aatatattcg acacatgcgt tttgactgtt
     1261 ttcagtgtga taaacaattc atccgcgatt tcctgattac ttttcccttc agaaatcagc
     1321 atcaaaattt cattttctcg attcgttaat tcttcatgca atactggatc atttcgccga
     1381 ctcatttttt ccatcatttt tgttgtcact tcaggttcta gtactcgctc gccgcgttgc
     1441 gtagccctta tagcatcagc tatttcatgt gctgtagacg tcttcaacaa atatcctgct
     1501 gctcctgctt caattgcagg ataaactttt tcatcatcaa taaaacttgt gacgataatg
     1561 attttggctt ttggccaatc ttttaagatt gcttttgttg attcgatgcc atccatttct
     1621 tccatgacta gatccattaa aatgacatcc ggtcgaagtg ccatcgcctt ttcatagcct
     1681 tgacgtccat tttcagcttc tccaatcact tcaatgtctt cttggataga taaataagaa
     1741 gagacgccta agcgtaccat ttcatggtca tctactaata aaacttttat catctttatc
     1801 tatgcctcct tcatcaaagg gaccttggtc tccacgctcg taccttgccc tttaaaacta
     1861 atgattttta ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa
     1921 cttcccgttt tggtttcatt catattaaat cctgtcccgt cgtcaatgat acgtaataaa
     1981 agattgttat ctattttatg caaatagact tctaattcat tagccttagc atgtcttaat
     2041 gtgtttgata acaattcttg aacgattcgg aacaagtgat cctcaatgga actagttaat
     2101 tttacatctt ctacctccca ttttagcgaa atctggattt tattctgcaa ttccttcaat
     2161 aactgctcaa tgccttgttt taaacttttt tcttctaaat tgactggacg cagatgaaga
     2221 agcagcgcac gcatctcaga ctgggaagcg ttgatgatct ctgccaccat tttcaactgc
     2281 ttacggaaca tttctggtgt ctcacttttt tctgcttgct ccgtcaatgc cgacatcatc
     2341 atcatcgctg caaataattg ctgtgaaact gaatcatgaa gctcacgagc taatcgatgc
     2401 ctttctaatt ttaaaatctc ttctttggtt tgtccatcta catattgagg acggctagtc
     2461 ataatctgta attcactgga tacttcgatc attttttctt ttattttagt aatttccata
     2521 tcaatatctt tgatgtacag atcatccgac gcgcttggga tcggctgatg caaaagtttg
     2581 gattcataat tgccagaaga aagtgcacga agcttttctt cgattttccc gtattttgcc
     2641 ttttgaataa gtgataacaa tagaaatacg atcaatccta cacctaatga aattgccatc
     2701 aaatggaaga taagcggaac gtataataaa cgcgcacgca gcaattctcc ccaccaatga
     2761 ctttggttac tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca
     2821 atccccgaat aaacagctag cattgctttg gatattcttc ccatcatatt cgtatcacct
     2881 cgatatctcc aaccaaagta ttggtaatga tcttcaatct gcgaggattt tcatcataat
     2941 cactgctgca gattttgatc tgttcgtttt ttaaggaaaa ctgctcttct tcaaacaaga
     3001 catttcctac taatgtcgca tgctctaaac gaatagccac tcccaggggt actaagatcc
     3061 gtgttctgcc tatccctttt cttacaatca caatattatc atcttttggc aaaagtgtat
     3121 tgcccaaatc gatgatagta tcaccagaaa tgatggcaat gttgatatca tcccattcat
     3181 agacgtcgtt tccaatacgt tggttaccaa ataattgctg tcttttacgc tcattattat
     3241 gtgtttttaa ttgctctgtt tggaccatca tgatctgttt ttttcgccaa aaagcatttt
     3301 ttgtcaaatc tattcctgaa atctctacac ctttcagccc gataaataaa acggccaata
     3361 caaccatcat ccataaagca ggactgttga ctaagctaaa aaaaatgacc aagctcccta
     3421 agatcaattg aaaattttga aatttagtac gaggatattt gcgcatagct agatatatat
     3481 tgaaaatacc gaatattact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag
     3541 caaataacag cagcagtgct tcgactacca caaaaaaacg ccaagaacta ttcatcgctt
     3601 tttctccctc ctgacacatt ctcatttcat catacctatt tttttgactg ttggctatag
     3661 gactttagat gtaagatcgt tcagtgaaac ctgcaatact caaagagaaa agatctagag
     3721 tgcgtcatca gtaagatgaa ctctctagat ctttcagtca gactacgctt gttctacttt
     3781 gacgatttta actaacatat cgccacctgg tgtagcaatg gttacttcat catttttacg
     3841 tttgccaatc aatgcttgcg cgataggaga atcattggaa atctttccag aaaacgggtc
     3901 tgcttcagca cttccgacaa tcgtgtactc ttcttcatct ccatcaggca actcgatgaa
     3961 cgtaactgtt ttaccgatcg atacctcatc tgcatctacg ccgtcattat cgatgatttg
     4021 tgcaaaacgg atcatatttt caattgttgt gattcttcct tcaacaaatg cttgttcatc
     4081 tttagctgat tcgtattcgg agttttcgga aagatctccg aaaccgcgag cgattttgat
     4141 tcgttcaatg atttctccgc gtttaactgt ttttagttct tctaattctt tttctaattt
     4201 ttctttacct tctagagtca ttggataaac tttctctacc atgtgtaacg tctccttttt
     4261 tcgataaaat accgcaaaag gtaatctttt gaggaatata gtccaaaaaa gattaccacg
     4321 cttttcttaa aatgtaaatg tttttctaaa aatgatcaat gactagcccc tgtttttcta
     4381 agaattattt acatattttt gcactagttc catatgttct tcatacgttt tagcgaaata
     4441 aacatttcca gtgctcgtat ctgctacgaa ataataatag tcattttctg ctggttctaa
     4501 tactgccttg attgcctctt cgcttggatt gtcaaaagga ccaggcccat agccggtatt
     4561 ggtatacaaa ttgtatggtg aatctacagc tgtatcttca tacgtcacca attctttatg
     4621 ttctcctaat gcatataaaa cagagatatc tgactgaagt ggcatatttg ccttgattcg
     4681 gttaaagaag acttgtgcaa tatttttacg atcattttct tttacgcctt ctttttcaac
     4741 tagagaagcc aaagttaaaa cttcttgtac agtcaaattt ttctgtttga tagctgaata
     4801 atagttttgc atgaccgtat ttgttttgtt caccatctca atcacaagat ccttaagcga
     4861 agtactcgtg tagtaatcat aagtagctgg gaataagtag ccttccaaac ggtattttgt
     4921 gttctcagct ttagaggcac tttccaacaa ttctgggaat ttttgataca actcgttaaa
     4981 gaaagtctca tcatttacta aatctaaaaa ttctttcttg tctttaccag ttaccttcgc
     5041 tacccgctca gcaatctgat cgatgtcgta gccttctgga atggcaattt tcccatctgc
     5101 aacttttgtt ggctcagatg tgccgccttc ttgcaactgc ttgccaatct catcaagagt
     5161 catattaggc gctaactgat aataaccagc ttgaaagcca gttaagtttt taaattttgt
     5221 ataataattg aaaacaatcc cacttttaat aatattgtct ttctccaaaa tttcgccaat
     5281 ctgcttatta gaagagccgc ttgggatttc cacttggact aattggtcgt ccgatttatc
     5341 taaaggcttc aacccgctat ctacataacg atatacggta aacccgagaa atccgccaat
     5401 aatcaataat gtaagagcga tcactaaaat gatcttcctc acgatcttat cttctttttt
     5461 acgcagattt ctccgcttgt cgattatatc tgctgtagct gccttactgg cttctttttt
     5521 gtctttgctt gggtgttgtt tgtttcctga caaaacaact cctccttact atcatcttcg
     5581 taactctgtt tattatacat gaaatttcta ctgccgtgaa ttattttacc aaatattatg
     5641 tcaaaattgt aagtggcaaa tgctcttttt gaggattaca atgaaaaata gttaaaaatg
     5701 gataggaagg atttaatatg gcaaaaggaa aacatcaaaa aaaacagcag atctctcttt
     5761 cttttctcgc agttttgttg ctggctgtca gcttcttttc aacaggcatc attacaggat
     5821 ggcaattaac aaaacaacct caaccacaaa tttataaaac ggaacccgca caacttgctt
     5881 ccaatttaac atctaatgag cagatgcttg ccagcaaaat tgatcaatta ctgcaaaaat
     5941 cagattatat cggttcgatt tatgtacgca aaaataatcg agtgatatta gaaaaaggat
     6001 atgggtacgc aaatctacgc ataaaagaat cgaattctcc ttctctttac tatcaaatcg
     6061 gttcgatcca aaaagcgatg actgctttac ttatcctaaa acaaattcaa tctggcaaga
     6121 taacttttga cacaaagcta tctgaatttt atcctcagat tcctggaagt cagcaaatct
     6181 caatcaaaaa tatgctttac atgcgctctg gtcttcatcg aaccgcttct ccaaaaaaac
     6241 ctatgtcaga tagtgagatc attcagtttg cccttcatca tttgaaattt actggttatg
     6301 atacgtatca ttatgaacca ttaaattata cattgcttac aggggtattg atcaaactaa
     6361 tgaatcagcc ttatgaaact cttttaaaaa aagaaatcat tcggccactc catctagaac
     6421 atacagattt ttatgaaaat gtaaaaaatt cccctcagca tgctgtatct taccaaatgt
     6481 ctgcaactga cgattattca aaagcattgg tcgagcctga aacagacatc cgaaatgaat
     6541 taggcacagg aaatataagt atgtctgttt atgacttgaa caaattcttt accagtgttc
     6601 tgtctggcga tattatacca aaagaactac ttttctcatt gtggcaaaat aatggtgata
     6661 aacatcctta cagcggcggt gtttacagtg gaaatgatta tatccttgct cagggaaata
     6721 tcaatcgatt ccatgcagta gcggcgatga aaaaagacac aaaagatgca atcgtcatgg
     6781 aaagtaatat ccaatctgat aaaaacatca aattgccggc cactgattta agaaatcaga
     6841 tttacgaact gatggaaggt gttaatttaa aaagctaagt cgagacaaaa aaaccagcag
     6901 gatgctggtt ttttgtttca atcgatttct gtctttaaat aacgttccat tccgttcaat
     6961 atatcaaatt tgggttgata acctaaagaa cggatctttg aaatatccgc taaagaatca
     7021 cgaatatccc ctgaacgctc cgcttcatat ttaagcgcta gctctgttcc taagatttga
     7081 tcaattgaat gaatcaattc aagtagcgta gtgctttttc ctgtaccgac attgaattgc
     7141 tgtcctaaag ctttttcttc cttagcaaca agtaaaagtg cttgtataac atcttctata
     7201 taaacgaagt ctctagattg gctaccatca ccaaataaag taaattctgt tttttctcct
     7261 gccaattgtt ttttgtatcg gtccactaga atcgaaataa ctccgctata tggagagtta
     7321 ggattttgat ttggaccata aacgttaaaa aatcggacag cactagtcgg aacatcatat
     7381 aaatgacagt aatccaaaac atattgttct gctgcaaact tatcgatggc atatggtgtt
     7441 aaaggacgga tgaccgattc ttcttttttt ggcaacgttg gttcatcgcc ataaactgca
     7501 gctgatgaac taaataccaa tcgttttaaa ccagattgat attttcgtac caattcaagc
     7561 aataaaagcg cactgtcaaa atttacacga tgagtttcta gaggacgttc tacagaatct
     7621 gccacactag ctacagcagc tagatggaaa atataatcga aatggtattc tttcatgacc
     7681 ttttccatca aatttttgtc agcgacatcc ccttcaataa acgtgatgtg cttcgattca
     7741 tccagattct cttttttccc catcgataaa tcatcgatca caactacatt gttttctttt
     7801 cctagataat tcgcgagcgt cgagccaatg aagccggcgc caccggtaat caagtaattc
     7861 aacatttttt ctcctctttt caagataaaa tttattacta aaaatagcta gaattatttt
     7921 tgctattaac tcttcctaaa aatagccaaa aaacaaacgt tcctacagtg ttttcataga
     7981 ctaattcatt gttaagagct gcgtatagtg cataagataa aataactaaa cagtaaacaa
     8041 aatgataatc tttaccaatg catttattat agatgattcg ataaacattc agcacaaaac
     8101 tctttattaa aaaagccatg aaaggaatta ttcctagaat acctgtcgat gttaatacat
     8161 tcaagtatgc attatgaatg gcaattccta ctttcgcaat atatccatct ggaataattg
     8221 actgtgcata agatactaca tttttcggag agactccgat aagccaactt gttttaaata
     8281 tttcccatgc acttttccat atagtaattc tagcattaga aatatctgta ttttcagcta
     8341 cgtctttcct aacaagagta ttatcaggtg tttgagtatt attaatatgt tgactattat
     8401 tcattaattc tttatgacgc tctgtaaaat taaaaaaatt tatttctttt aaatgaggta
     8461 ataattcttg aaaaagtatc ttagttgcat taaacaaagc gaataaaatc agaacagaac
     8521 caatagaaga tactatagca aggatatagt tttttaaatc tttttttgtt ccatttcgtt
     8581 ttaacacaac aataaaagat gatatggcaa ctattattat acttaccaca taggtagatc
     8641 gtgacccact taatac
//
LOCUS       DAYYAJ010000122         8399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000122 DAYYAJ010000000
VERSION     DAYYAJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.122"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..447)
                     /locus_tag="ACHATZ_002460"
     CDS             complement(85..447)
                     /locus_tag="ACHATZ_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404937.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(521..901)
                     /locus_tag="ACHATZ_002461"
     CDS             complement(521..901)
                     /locus_tag="ACHATZ_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8404938.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            983..2158
                     /locus_tag="ACHATZ_002462"
     CDS             983..2158
                     /locus_tag="ACHATZ_002462"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8404939.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2235..2819)
                     /locus_tag="ACHATZ_002463"
     CDS             complement(2235..2819)
                     /locus_tag="ACHATZ_002463"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8404940.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3020..4018
                     /locus_tag="ACHATZ_002464"
     CDS             3020..4018
                     /locus_tag="ACHATZ_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8404941.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4130..4639
                     /locus_tag="ACHATZ_002465"
     CDS             4130..4639
                     /locus_tag="ACHATZ_002465"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8404942.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4669..5328)
                     /locus_tag="ACHATZ_002466"
     CDS             complement(4669..5328)
                     /locus_tag="ACHATZ_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8404943.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5330..6094)
                     /locus_tag="ACHATZ_002467"
     CDS             complement(5330..6094)
                     /locus_tag="ACHATZ_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8404944.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6102..6779)
                     /locus_tag="ACHATZ_002468"
     CDS             complement(6102..6779)
                     /locus_tag="ACHATZ_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8404945.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6816..8201)
                     /locus_tag="ACHATZ_002469"
     CDS             complement(6816..8201)
                     /locus_tag="ACHATZ_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8404946.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
BASE COUNT         2535 a         1653 c         1456 g         2755 t
ORIGIN      
        1 acatttcatt gtattttttg gaccaaacaa taaaaaaaga agtttgaaaa aaattcaaac
       61 ttctttgttc gcttaatctg tattttagaa gaaaggcact ccctctactt cgtaagttcc
      121 aagaacagag cgcatttgac ctggttcttc ccaaatatat ccttcgccta attcttctct
      181 ttgattcttt tgttcagttt caaagacaaa caacggaaca ttttcctctt taatatgtgt
      241 atttgtcaat tccatcaaac taatcttgct aacatcgata tgaactattt cttttaagaa
      301 attaagtata ttcgctaaac cagtctttcc ttcagaaagt tccgctaacg caaattccaa
      361 cttgtcatca attgaatgga ccaaaaattt tttagtacca tcctccaaat gcaacataat
      421 tgtaccagca actcttttgc ttgccaaaat ttccacctcg atcaacttgt tctatggctt
      481 acataaaaat tctagcacat tttcagaaaa aatacacttt ttatttctta ttttctttcc
      541 gttctagttc agataatgct tcttctatcc aaataggtaa ctgctccgca atagtggtaa
      601 atccgatctt acgattttta ttcgtgaaat acctttttct gcgatatgga acagctggat
      661 ttgttttttc taacgttctt aaagataaac tgattttttt ggaatattca tcaatgtcga
      721 tgatctgaac tgttactggc tgtccgactt ttaacaatga atggatattt ttcgtataac
      781 cggattgtac ttctgaaaca tgaataagtc cttgcgtctc gttatcaaga gaaacgaatg
      841 cgccataagg ctgtatccct gtcacttttc cttcaattat ttgtccaatt ttataagtca
      901 tctacttccc tctcttcgca tttatcatta tactgtaaat agctcaaaaa aagaagcgac
      961 gaaaatgact ggaggtctta agatgatcga atttgataaa cagatttcaa gaaaagatac
     1021 agaaagtgta aaatgggacg caattgctga gacatatcaa atggatgact tacttccttt
     1081 gtgggtagca gatatggact tcttgtctcc cggtggggta gctaaagcgt ttagtgaata
     1141 catcaaacat ggaatctttg gttatgccac tctgtctgat aaactatatc aggctatcat
     1201 tcattgggaa cgccaacacc atgctgtgga gttgacgaaa gaaaatatcg tttttacaag
     1261 tggtgtgtta accagtttag ctgcagcagt acaaactttt acaaaaccag gcgattctat
     1321 tttgattcat gacccagttt atcctccatt ttcctctatt atagaaacaa atcaacgtca
     1381 catcgttcga agctctttgc tagaaaaaaa taatcatttc atcatggact tgtctgacat
     1441 ggagaaaaaa atcgaagaaa acaatgtaaa agcaatgatt ttatgcaatc ctcacaatcc
     1501 tggagggcgt gtttggagca aagaagaatt gaaacagtta agcgaattgt gtttaaaaca
     1561 tcaagtgatt ctgtttagtg acgagatcca tcaagattta actttattcg atcatacatt
     1621 tacttccatg ctaacgattg atcctaattt agatgatcta ctagttgctt ttacttccgc
     1681 aaccaagact ttcaatctag ctgcaatcaa aaattcaatg gtatttatca aaaatccaga
     1741 gatgaaagct gattttgaaa aacatttagt gatgaaccaa cagcacgaaa tcaacacttt
     1801 tggactgatt ggaacccaag cggcttatga gacgggtgga gaatggttaa gccaactgat
     1861 tccttatcta gaaaaaaatg ttcaaacagt taatcagttt tttgaacagt atcttccgaa
     1921 agtgagaata atgaaccctg aaggaactta cttaatgtgg ttagatttta ccgcctatat
     1981 ttcagacgac caagaattgg agaacactct tgttcggaaa ggaaaagtcg tattaaatcc
     2041 tggcatcacg tttggtcctt ccggtcatgg gcatatgcgc ttgaatattg cttgtccaga
     2101 agaaacttta ctggaaggac ttaatcgaat caagtatgct ttagagacaa tcgattaaat
     2161 catttattca aaataaaagg acgagacctt actaggtttc gtcctttttc ataattactc
     2221 cgtaccatac caaactattc tgaaatttca attgtttcaa tcactacatc atgtacaggt
     2281 cggtcttgtg gaccacgttg tacatttgcg atttcatcta ctgtgtccat cccttctagt
     2341 acatgtccaa agactgtatg tcggaaatct agccaaggag ttccgccacc tttatacgct
     2401 tcaatgattt ctgatgggaa tccagcacct tctagttgtc caagcatatt agctggcaca
     2461 tttgtgttat ttacaataaa gaattggctt ccgtttgtgt ttggacctga atttgccata
     2521 gataaagctc cgcgaaggtt gaacagttca cgactgaatt catcttcaaa actttcgcca
     2581 taaatacttt cgccacccat tcctgttcca gttggatcgc cgccttggat catgaaatca
     2641 ggaataacgc ggtgaaaaat cacgccatca taataacctt ttttggaaag ttcaatgaaa
     2701 ttttgaaccg tcttcggtgc tagttcaggg aataattgaa cagtaatgtc accacggttt
     2761 gttttgataa cagcttttgg tcctttttca ctttttaaat ctagttgtgg gaatgccata
     2821 ttgtttcctc ctaatatttg catacttcat aataataata acataatacc taacctctcg
     2881 taaaaatact agatttgttt tcttttattt ttcatcacta gatacattct ataacttacc
     2941 gtttcgattt cgaattcaaa catttttgag aagcattctc aattaaattc tgatatagtg
     3001 tgaaaaggga ggaattttta tggaaatcta tgacattaca atcatcggtg caggtcctgt
     3061 agggatgttt gccgcttttt acgcaggaat gcgccaagct aaaacaaaaa tcattgacag
     3121 tctacctcaa cttggtggtc aattagcaac tttgtatccc gaaaaatata tttacgatat
     3181 tcctgggtat ccagcaataa aagcaagtga attaatcgat caattagaaa aacaattaac
     3241 tacttttaac catacattcc atttaaaaga agaagttctc tcgcttactc gtgaagacga
     3301 agttatcgag attactacaa ataaaggcat ccattattcc aaagcagtca ttttagcttt
     3361 aggaaatggt tcattccagc caagaaaatt gaatttagat aatgctgaat catttgaaaa
     3421 ccacggattg gattatttcg tgaatgattt gatgaagtat gctggaaaaa aagtcgctat
     3481 tgctggaggt ggtgattctg ctatcgattg gacgttgatg ttagagccta ttgcaagcga
     3541 agtttacttg attcatcgtc gcccggaatt tcgtgcgcac gaacatagtg tatcacgctt
     3601 gaaatcttca tctgtcaact tgcttactcc ctatctcatc gatggattat caggcaataa
     3661 tggggaactg acagatatcc ggttaaaaaa agtgaaaagt gacgagacga ttgatttgat
     3721 gattgattct ctgatcgtca attatggatt ctcttctaat ttagaacatc tttcttcttg
     3781 gggacttgat agtaccagaa atgccattac tgtaaaatca gatatgtcta cttctattcc
     3841 aggcgtttat gcagctggtg acatctgtac ctatgaagga aaagtgaaac tgattgctac
     3901 tgggctcggt gaagcaccta ctgcagtgaa caacgcttta cactatatta atccaaagga
     3961 acgaactcag cctggtcaca gcacaagctt atacgataaa aatcttcgtc ctagttgatt
     4021 ttttatatct ctttgcattt caagttaaac ccgaaagcag tttcttgcat ttttttcatg
     4081 ttccgttaac atgaaataga gaaagaaaaa ataaaggggg agcaataata tggtcgtcaa
     4141 aactgagact ttagaagaaa aggcaagaga actattgaag gaacgtggcg taactgtaga
     4201 agatatcgca caacttgtca tgtttttaca aaaagactat atttctgatt tgactttggc
     4261 ggattgtact gaaagtgtca acagcgtgct aacaaagcgt gaagtacata acgccattat
     4321 cactggaatc caattagata tattagcaga aaagaaaaaa ttgatgagtc cgttgcaaca
     4381 tattatcgaa gatgacgaag gcctttacgg tatcgatgag atcctagcct tgtcaatcgt
     4441 gaatgtttac ggtacgatcg gctttacgaa ttatggttat atcgacaagg tgaaacctgg
     4501 tattttaaaa gaactaaaca gccacgatgg cgttcatgtc cataccttct tagatgatat
     4561 cgtcggagcg atcgccgctg ctgctgccag tcgattagca cacaaagaac cagaaaaaag
     4621 aagtcgcctt actcaataat tcaacaaaaa aagaaagaaa acgtctattt agacgagttc
     4681 tttctttttt gtttgtttca agctattttt tcctaagaaa agaaaaacag caaaaaataa
     4741 ttctaataat acaaaaaata agataaagct atgcatgaaa aattcttctg gaagtacatt
     4801 gatgattgga tcagtaacgg gatcgaataa ccagtcatcg ttgttgaaaa acgtttcatg
     4861 aaaaaggata aaaaagcgat caaatcctat catcatgaaa aaaccgaaaa caactggcag
     4921 taacatgccg atttgaaacg gacgaatcaa ccgccaaagc cgatcattct tttgcaaata
     4981 tttgataaac aaaatactcg ggatcaaagt gatgaaaaac acagcataat ccactaaaaa
     5041 caaaattttc acttcataaa agtgatgggc tccgctagca gaaactggga aatctggcaa
     5101 gctgaggatg gttttgaagg gattattgag ataattcatt aggtgatcaa aatttttcag
     5161 taacgtctct tgatctaaag aagtataatc caaaatttgc aaatgtccga tatcaaatac
     5221 gtatagtggt cgaaagttga tcgtcacacc aactgccaga ctgatcagtg tcagaaatag
     5281 gctgaacaat ccagcatatt ccatccactg ccatctcgaa tttttcatat tagaagctcc
     5341 attcatccag tgattgtttc acataggtag ctgggatagg caacccaggt acatcttctt
     5401 cttttgtaaa acctgataaa acgagcaaag tatctatatt attgcgtatc ccagcttgga
     5461 tatctgtttc atagttatct ccaaccatga ttacttcttc tttttgcagc cctaatactt
     5521 caacggcttt atccataatg atggcttctg gttttccgat gtagatcggc ggcgtttggg
     5581 ttgctgtctc tacaaaagag atgactgacc ctgctccagg aagaaggccg cgttcagtag
     5641 gtatattctt atctgggttc gttccaatga aagtagcacc tttttgaata gcaagtgttg
     5701 ctttaactac tttttcatag gtcagataat tatctaagcc taccactaca tagtcaggag
     5761 cctcttcttc ccatgtaaat ccggcagcta aaatcaagtc aatcaatcct gcttctccga
     5821 taacatagac ttttttccct tttccatctg ctttcataaa atcgattgtt gccaaagtag
     5881 ctgtgtagac tgtctctgga gctacatgaa tgtcaaactc atctgctaga cgattagcta
     5941 ctgtttctgg tgacttagtc gtgttattgg taacaaacaa gaaaggtaat tttctttttt
     6001 gcaattcttc cacaaatcgt ttaccggccg gtatgggttg tgtcccacga taaatcgttc
     6061 cgtctaaatc aattaaatat cctttatagt ccattcatcc actactcttc cctttttctg
     6121 atcgtaaatg ctgtttttgt tttttctact tgtttaggtt gttcttgttt ttttactttt
     6181 tcagaggttt ttgctgactc tgtttttcga tttttaataa ccggtttttt gcgattagat
     6241 gcgactggtc cttttttctc atcgatatac gcacgattat ggtttttttt cttttttcgg
     6301 cgctgagttg tcttttcttt tttaccgccg attctctcga tgacaaaata cgcacaacca
     6361 aaattgcaat attcgtaaag atagtcttcc aaggaatcga ttcgctgatc tggatgcgcc
     6421 cgtcgattat ccgcttggaa aaatcctttt aggcgtagtt gttcataacc ccaatctcct
     6481 acgatataat catatcgggc tagtacatca ctgtaacgtt ctccaagctt ttctgcatta
     6541 aatccttcac gataatcgga cacaagccga taatgtcttt cacctatcaa aaaattactg
     6601 tcatctaaca gtgtcactag ctcgcctttg actttttctg ctttaggctc ctcaacgatt
     6661 tcttccaaag cagctgtcag ttcttcggtg actgttgttt cctgttcagc tgatgatttt
     6721 ttcttagcaa tttctgtttt atgctcaacg ttctgctgtt cattttgttt tgcagacatg
     6781 cgttcaccac ctatcttttt tattatacct tgtttttatt tgattgggta gtgagctttc
     6841 aagtaatctc ctaccacact tcggataaat tcagggaaca aaaattcatt ttttccagca
     6901 atctcaatcg ttggaaaaaa tggaacaaac atcaaatgat ctacggtagt aaaagtataa
     6961 tttttattca gatcgattgg cttgttcttc caaaagactt gatggttaat agaatcatat
     7021 gtgattccat tatagaccat ctgcccaaaa atcttaccgc gaaatcccat tcccttcata
     7081 ggatagttac gtaaaaagtt acgatttttt tctatttcta aaactaagcg gatcagatca
     7141 tttccaccca atgtgacatt taacaagtgc atcggatgcg gcaacgcagt gtgaagctga
     7201 tcctgattga cccgcccttt tggaatttca gttaaaaaca gaccactgtt taatattgct
     7261 gcttccgtct ttcctcgttc tttcaccgcc tcaaaggcta cttggataaa cgaatgctca
     7321 ttgaagatat ctagactcag agaaaatggc aaatctgcta cttctttttc ttttaataat
     7381 tcatgcccac gttctagaca actatttatt tcttgttcat cttctgtaaa agctgtcatt
     7441 gtttcggtag ggatggcttt ggctgaatgc ttaaggattt ttttctcttc atcgatggtt
     7501 aaatggactt caccgacata ctgcccgtac tttccagcgg ctgctatttg gacatcattg
     7561 attacgtgac cttctctgaa taaatgatgg gtgtgcgatc ctaaaataac atcaattgct
     7621 ggtatttctt ttgcaatttg gaaatcatcc tgaatcccga gatgactcat caaaactaaa
     7681 acatccactt gtgaacgtaa atcttcgact aattctggca aaatatcata aggattccgt
     7741 atgtcccagc cattcggatt ataagtaagc ggaaatggag cagtaaatgc aattagtccg
     7801 attttcgtct ggtgtttcgt tgtaatgatt ttatatggtt tcgtccatgc cggtggctgg
     7861 agcgtatttt tatcgaataa gttatccagg atgatatcaa actcagcatg atcgtataaa
     7921 tgattcaact cttctttgga atttcctacg ccttcattgt ttcctatcgt cgctgcatca
     7981 taaccaactt cattcatcag caagatattt tcctgtccat tcgttgcttc tgacaatgga
     8041 tgccaacgat ccacaaaatc acctagatct actgcaatcg ttgtcgtttt ttctttcttt
     8101 tcgttttctc gtttacgttg atccaaaaac cttctgatcc ttggccaatt ttctaaatga
     8161 gaatgcagat cattcgtatg taaaatcact attttttcca tgtaccctct cgcctctctt
     8221 tatctttatc ataccatagg cagctttttc ttccattgaa acaagctcta tttcagagtt
     8281 tttcctgcct tgaagcaaat tgtataagta caatattcga taaatcagtc tggctaaaaa
     8341 atcaggaaca ttcattttta cagtactata aaaataaaaa aaacagaccc gaaagtctg
//
LOCUS       DAYYAJ010000123         8365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000123 DAYYAJ010000000
VERSION     DAYYAJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.123"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..517)
                     /locus_tag="ACHATZ_002470"
     CDS             complement(<1..517)
                     /locus_tag="ACHATZ_002470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404947.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFI"
     gene            complement(510..1139)
                     /locus_tag="ACHATZ_002471"
     CDS             complement(510..1139)
                     /locus_tag="ACHATZ_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8404948.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(1132..2250)
                     /locus_tag="ACHATZ_002472"
     CDS             complement(1132..2250)
                     /locus_tag="ACHATZ_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8404949.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(2252..2884)
                     /locus_tag="ACHATZ_002473"
     CDS             complement(2252..2884)
                     /locus_tag="ACHATZ_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8404950.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2895..4712)
                     /locus_tag="ACHATZ_002474"
     CDS             complement(2895..4712)
                     /locus_tag="ACHATZ_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8404951.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4725..5489)
                     /locus_tag="ACHATZ_002475"
     CDS             complement(4725..5489)
                     /locus_tag="ACHATZ_002475"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8404952.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5517..6215)
                     /locus_tag="ACHATZ_002476"
     CDS             complement(5517..6215)
                     /locus_tag="ACHATZ_002476"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8404953.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(6227..7006)
                     /locus_tag="ACHATZ_002477"
     CDS             complement(6227..7006)
                     /locus_tag="ACHATZ_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8404954.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7022..7966)
                     /locus_tag="ACHATZ_002478"
     CDS             complement(7022..7966)
                     /locus_tag="ACHATZ_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8404955.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8012..>8365)
                     /locus_tag="ACHATZ_002479"
     CDS             complement(8012..>8365)
                     /locus_tag="ACHATZ_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404956.1"
                     /translation="VSNWQQAVDDVVIKEKVTSADVARVRENMSFFKDSPWKTVVMQY
                     LGFADTQIAQVTQLDQLFDTMLKDGQVTATATYDQYLTALSQIEQIRNEKISAAYATK
                     AQTVAQQMGYSNISY"
BASE COUNT         2270 a         1736 c         1407 g         2952 t
ORIGIN      
        1 ttatgaattt tttgaaataa tcgcattctg ttataactag actagaatta ctgagatatt
       61 gatctcgtag ctttttccat aaatcagtaa aaaatttata tttgttccct attcccggca
      121 ttgactctgg ccataaatca tacacatcaa aaataactcg attgcatcta ctaaacttag
      181 caataaaaaa aactaaggta ttaggtggta ttctacaata aataatttca ggcttttctt
      241 ttcgaataaa taataatgtt tttattgaaa accatatatg agataaaatt cgcattacgg
      301 atatattttt tttatatccg atcgttttta tcacatgacg atttttaatc atatgccttt
      361 ctttactgga tagaaattgt tttcttatat gtgagtaatc agatacaaca ttaaaatatt
      421 cgatactgtt ttccgtcaga atttcctcta atatattcgt tcgactgata taatcaaaag
      481 gatgaataag cagccacttt tttttattct cactcatcta tcttcgcacc ctcaaatttc
      541 tcagtagtcc cacttgctac tgatgttatt ccatcacgga caataacttt ctttagcgtt
      601 aaaaaaaata tttttatgtc tttcatcatt gaaatgttct ccacatattc aatatctttt
      661 tcaaattttt ctttccaact aattgcatta cgaccactga cttgagctaa tccggttaaa
      721 cctgggcgca caaaatgtct ttttcgttgt tcattactgt atagtgaaag atactcaact
      781 aacagaggac gcggtccaat aatactcata tctcctttta aaatattcca taattctggt
      841 agttcatcaa gactagtcga acgcaatatt ttaccaaatt gagtcaatct gatatcgtct
      901 gataataaat taccttcttc atctctttga tctgtcattg ttcgaaattt atacatcaga
      961 aaaatctttt catgttttcc aggtcgttgt tgtttaaaga tcactggcga tccaagcttt
     1021 attcgaacta ggattgcaac aaacgtcatc agtggccata acattattaa acctaaaatt
     1081 gaaaaaagaa tatctatcaa acgtttaaag aacttttcgt agatacctct attagacatt
     1141 ttgcatcact cgcttaattg tattagttac tcgatacaaa tcatcatcgg tcattttaga
     1201 atctgaaggt aaacagatac ctttttcaaa caatgtctga gcaacatctg taccaatata
     1261 ctcatactta tcgaaaactg gctgtaaatg cattggtttc catatcgggc gggcttctat
     1321 attattttta tctaactcat caataagatg attcgtattt attcccttta ctgttatgac
     1381 acttaaccaa taatttggtt cattccaatt attttctggc atgaattgta tatttgacaa
     1441 tttagacaat tcttttttat aaaaactatg aatatatcgt tttttctcaa ttctttcatt
     1501 taaaactttc aattgtcctc gaccgatacc tgcaacgata ttgctcattc gataattata
     1561 gccaatttca ttgtgttgat aatggcgtgc cttctcacga gattgcgtag cccaaaacct
     1621 cactttttcc accttttctt tgtcattaga cacaagcatt ccgccacctg aagtcgtgat
     1681 tatcttattt ccattaaaag aataaattcc atagtcgccg aatgtaccag tatattttcc
     1741 tttatatgtt gttcctaatg attctgcagc atcttcaatt aaaacaacat tatgttcttt
     1801 gcatatttta gtaatttcat ctagttcagc tgataaaccg tacaaatgca ctactaatac
     1861 agcttttgcg ttaggatatt tttctagcgc tgttcgcaaa tgttcagggt tcatattcca
     1921 agtttcgtaa tcactatcaa caaatactgg aatagctcct tgatagatga ttggattggc
     1981 agtagctgag aacgtcaaag actgacaaat cactatatca tctttaccga ccccagccgc
     2041 ttttaaagct agatgtatag ctgctgttcc agaagatagt gcagctgcat gactagcacc
     2101 gacatattct gctaattcct cttcaaaacc attgacattc atacccaaag gagccaccca
     2161 atttgtatca aatgcttctt ttacatactc ttgttcatat ccttcatcac tcatatgagg
     2221 agatgctaat aatattcgtt tattttccaa cttaatccca tctttctatg actttggtag
     2281 gtacgccaac atttttagtc ttattattaa tattttttat tactacactt cccgcaccta
     2341 taatgttcat atttccaatt cttagatttt gtattatttt agtacctact ccaatttcat
     2401 ttttatctcc taatagaaca tgtcctgaaa tatttacact tggaaaaatt gaattataat
     2461 taccgatttg agtatcatgt ccgatagtag agccgatatt gatcatattg aaatttccta
     2521 aaactacgtc agcagtgtaa gtcgtatagg gcatcaaaat attgccaatt cctaactgaa
     2581 catttatgcc cactagtgca cttgatgcaa ctaaattagg gaaaatcaat ttattatttt
     2641 tatttatctt ttgatatatt ttctctttga tatctggcga agcgattccc agaaaaatag
     2701 cagtttcttc ttctaattct aataacgagt ctattgttcc caatacagtt tttcccacta
     2761 attcttgatt ttgcattttt aacgaatcat cgacaaaacc aatcaattca tatctagaga
     2821 aactttctag tagaaaagca atctctttgc caaatccacc agctcctaca ataattgctt
     2881 tcactttact ccccctactt attcgaagca ttcgcaaaag tgacaatgtc tttagccaat
     2941 tgttcatcgt cttgcggcag gctcttgaca aattccatca ctgtttgcat ggaataacct
     3001 ttgatgtttc ccacaaagat cttctcgtaa actgcttcat cgtttctttc cttatctaac
     3061 aatagttctt catatagttt ttcccctgga cggatacctg tttcaatgat ttcgatctcg
     3121 tcttcgctat atccgctcaa tcggatcatg ttttttgcta gatctacgat tttcactggt
     3181 tcactcatat ctaaaataaa gatttcgccg ccttttgcta acgccccaga ttgaataact
     3241 aaacgacttg cttcaggaat cgtcatgaaa tatcgggtca tacgaaagtc tgttacagtg
     3301 actggccctc cttgagcaat ttgttcccgg aatactggaa tcacactacc gcgagaacca
     3361 aggacattcc caaatctgac agctgagaat ttcgtacatc cttcttcatt caacccagtc
     3421 acgatcattt cagcgattcg ttttgttgaa cccatgacgt ttggcggatt gttggcttta
     3481 tctgtcgaga ccatgacgaa gttttttact ttcgctgctt tggctgcttc tgctacattt
     3541 tttgtaccga aaatattgtt tttgactgct tcttttgggt tatattccat caatggtaca
     3601 tgcttatgag cggctgcatg gtagacgata tctgggtggt attgttccat gatctcattg
     3661 attttcttgc gatcttggat atccgcaatg atcggcacga tttctgtagg acacttttga
     3721 tgatgcgtgc gtaattcacg gtcaatcaag taaatcgagt tctccccatg acctaaaagt
     3781 aatagttttg cagggtcaaa ttggatgatt tgccggcaga tttctgaacc gattgatcct
     3841 cctgcaccgg tgacaagaat gactttgttt gtgatctggt ctttgatgga ttcgatatcc
     3901 aactctacct catctcttcc cagtaattcc actacgtcga tggtttttaa ttttgatacg
     3961 ctgattttcc ctgaggcaag ttcttccata gaaggcatcg tattgacttt cacatgagca
     4021 gattcgacta actcgaagat tcttcgtaat ttttttcttg ataaagaagg gatagcgatc
     4081 gttaccattt ggatgtcata tttttcgatc aattccggta tatcttttaa tgaccccagt
     4141 actttttttc ctgaaagata ggtatttctt ttattcggat catcatccac gaatccaatg
     4201 acatggatat cttgagccgt tttagaacca agaaagctgt tgtataaaat acgtccacct
     4261 tcgccggccc caacgatcaa ggtattttta gcggaatctg ttgatagata cctcatattt
     4321 tttgtttcga tatagatacg ccagatcaat cggctgccaa tgatcagtaa tagcgaaagt
     4381 aggtatgcaa agatcactaa acgtaaactg tattgtttat ttatgaaaag caatatgatg
     4441 atacttgagg ccgcacttgc agataacgaa ccaaaaatcg caatgatctc tcttaaattt
     4501 gtataacgat tgattcgagt aaaaactttg aataaactac catagaataa ataaaaacca
     4561 atcgacaaac cgatagagat taaaataagg tccattggta tagctacaaa cggcttcatg
     4621 aatttagctg ctgcaatatt cgcagcaatc aacaacaagc tgtccacaaa taccaatgtg
     4681 gcaatttttc ttcttcttgt taatacaaac accctacatt ccccctaaaa taaaccaaac
     4741 ttcttctttt ttacctcttc atagactgga taactgatct catccccgtt gatcaggtcc
     4801 tttgcgactt gtttcatctg tgctactttt tccttaccaa agtccttggc gatttgctca
     4861 taacattctt ttaagtaaaa ggtacgttta gagaccccat gggcatcgga agcagccatt
     4921 tgcacgaggt tatgtttgac catcattttg gctgttttct ggatgtcttt tccaaataca
     4981 ccgacataac ttggtgcggt gagttgagcc aagcatccca tatctaggaa tggcagcaaa
     5041 tgatttgggt cttctcgaaa atgcgcgttg cgttctggat ggacgatcac tggtgtcttt
     5101 cctagttgta acaactcaaa aaagagccat tctgcatata acggcacttc cagtgtcgga
     5161 aattcgatta gaagatatgt atcatctaaa tctgtaaata aaatttcatc tcggtggatg
     5221 tcttcaatca gtgttccggt aatacgtact tcttgtcctt ccaataacgt caaaggcaat
     5281 tgtcttttct ctaattctgc ttgtaacgaa gcgactagag aaatgacttg tgatttttca
     5341 ttgctgtatt ttccattatt gtgatggggt gtgcataaaa tatgtgtgat cccttgttga
     5401 atcgcttttt cagccatggc gatactggca tctatgtttt ctgctccgtc atcgattcct
     5461 ggcaggatat ggcaatgtag atcgatcatt tctcttgtct cctctctttt tatttcttaa
     5521 ttcccgtagt aatagccagc atctctgctg tgttctgctc cattgtatac aacaccaagg
     5581 actcgcgctt gtaccatatc taacagttct tttgctttag tcaatgattc ttttcgtgac
     5641 gtattttccc gtacgaccaa taaggtgcca tccgtttttg aagacatgat ctgtgcatct
     5701 gttacggcaa cgactggtgg catgtcgaaa atcactacat caaatagttg tcttgcttct
     5761 gctaaaatct gatccattct tggcgaactt agtagttctg atggatttgg tggtttaggt
     5821 ccacttggca gaatcgacaa attgtcgact ggtgttcgtt ggatctcatc tgctacactt
     5881 cctgatgaac taagaacagt acttaatccg cttgcgttgt tcaacttgaa cgttttatag
     5941 atgacgggct tacgtaagtc tgcatcgact agcagtaccc gttgaccaga tttggcaaaa
     6001 acaacggcaa tgtttgctgc agtggtggat tttccttctc caggacctga tgaagtcacg
     6061 acaatcgttt tgatttgctg accagctgcg gaggcaaact ggatattcgt ccggatcgta
     6121 cgatattgtt cggcgattgg tgaagatggg tttgttaatg tgatcaaact gactgcatga
     6181 gtttgagttg ctttttgttt ctgtgttcgt gccatttttc ttcctcctag actcttgagc
     6241 ggcttctgcg tgatggggct gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc
     6301 cttttttacg gataccagcg ttcaattctt ttgctgacat ttctggaact gttcctaaga
     6361 ttgggaatcc taatgtatcc gtcacatatt tttcatcttt cactgtccga tcaagtaatt
     6421 cgaataagaa tgccagacct acgcctacca tcattcctaa cactaagcca atcactagat
     6481 tcaatttatt atttggcgaa acaggtgtcg tattggcaac tgcacctgaa ataatcgaga
     6541 ttttatcgac attcatcacg tctttggcgt tttcttggaa aacttgtgaa gttgtattag
     6601 cgatctgttg agcagtcact gcattcgtgc ttattgcctg gatcgagaac atttgtgaat
     6661 tttgtgactg attgacggag atcgcatctt tgatttgtcc agcagtcatt ttcacattgt
     6721 cttctgtttc taaacgatct ttgacttcat ttagtactaa atcactgacg atcatatctt
     6781 tatacgtatt gatcatttgc aggttcgtat tgacatcatt gacatttgct gtgtctgatt
     6841 gtggcagtgt cacgatcaat tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg
     6901 ttacgattcc tgagatggcc aagccagcaa acattgagat gatgattgtt accaagtgtt
     6961 tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc ttccattttt tctccacttt
     7021 cctatttatt ctctagattc atttgatctt ttaattcttt tttcacacga tcaagttctt
     7081 gatcgctgac tttttgatag gagatattat cttgcatgaa gccttctcct tgcagctgat
     7141 cttgcttgat cgtaccaaat gcctcccgat aatctaaagc gatttttttc atttcatcaa
     7201 agctcatgtc tgttttcata ttcgactcta gagcattcaa aacggattga tagttgctga
     7261 caccgtttaa actcaatact ttttttgcta caccttctac gatctttctt tgacgctctt
     7321 gtctaccata gtcaccattt ggatcttcat aacgcatacg agaataagat aatgcctgat
     7381 ccccgttcaa tgtaatttta ccgattggaa agtcatagcc atcttgtgag aaagtgaggt
     7441 cattattgac ttcgatacca ccaactgcat caacgagttc ctttagccct ttcatattga
     7501 tagagacata gtgatcgatt gggatgtcta catatttttg tagggtatcc atcgccatgg
     7561 cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt
     7621 cgacatacgt atctcgagga atgctgacga tcgttgtctg tttgtcttca ggactgactg
     7681 tggcaaccat catcgtatct gatcttcctt gatcgacccg acctaaatct ccagtatcaa
     7741 tgcccatcaa tagaacagag aaagattctt gtttgtccag atcaactggt ttcgttcgtt
     7801 ctccttgggt tcgttccact gattcgtaag ttttctcaat agaatgtgag agatccagat
     7861 ataattttgc tcctactcct acaattgcta atactaatac gataagaact gctaaaataa
     7921 tcagaaatat ttttttccct cttgattttc ttcttcgtct tgccatagtt tcctccgttt
     7981 gctaaccttg cttgaatcaa cgttttaact gtcaataact tatattgcta tagcccattt
     8041 gctgagcaac agtttgtgct tttgttgcgt atgctgcgct tattttttca ttccggatct
     8101 gctctatttg actgagcgcc gtcaaatatt gatcgtaagt agcggtggca gtcacttgac
     8161 catccttcaa catcgtatcg aataactgat ctagttgcgt gacttgcgcg atctgagtat
     8221 cagcgaatcc tagatattgc ataacaaccg ttttccaagg actatcttta aaaaagctca
     8281 tattttcacg tacacgagcg acatctgcac ttgtcacctt ttctttgatg acgacatcgt
     8341 ctacagcttg ctgccagttt gatac
//
LOCUS       DAYYAJ010000124         8232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000124 DAYYAJ010000000
VERSION     DAYYAJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.124"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHATZ_002480"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHATZ_002480"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8404957.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHATZ_002481"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHATZ_002481"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8404958.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHATZ_002482"
     CDS             2594..3379
                     /locus_tag="ACHATZ_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8404959.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHATZ_002483"
     CDS             3392..4243
                     /locus_tag="ACHATZ_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8404960.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHATZ_002484"
     CDS             complement(4367..4819)
                     /locus_tag="ACHATZ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404961.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="ACHATZ_002485"
     CDS             complement(5284..5652)
                     /locus_tag="ACHATZ_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8404962.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="ACHATZ_002486"
     CDS             5802..6284
                     /locus_tag="ACHATZ_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8404963.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="ACHATZ_002487"
     CDS             6321..7331
                     /locus_tag="ACHATZ_002487"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8404964.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="ACHATZ_002488"
     CDS             complement(7446..8159)
                     /locus_tag="ACHATZ_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404965.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2442 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt ct
//
LOCUS       DAYYAJ010000125         8201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000125 DAYYAJ010000000
VERSION     DAYYAJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.125"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..983
                     /locus_tag="ACHATZ_002489"
     CDS             120..983
                     /locus_tag="ACHATZ_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8404966.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1112..2734)
                     /locus_tag="ACHATZ_002490"
     CDS             complement(1112..2734)
                     /locus_tag="ACHATZ_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8404967.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2983..3504
                     /locus_tag="ACHATZ_002491"
     CDS             2983..3504
                     /locus_tag="ACHATZ_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8404968.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3690..5420)
                     /gene="spxB"
                     /locus_tag="ACHATZ_002492"
     CDS             complement(3690..5420)
                     /gene="spxB"
                     /locus_tag="ACHATZ_002492"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8404969.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5585..6403
                     /locus_tag="ACHATZ_002493"
     CDS             5585..6403
                     /locus_tag="ACHATZ_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8404970.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6506..6703)
                     /locus_tag="ACHATZ_002494"
     CDS             complement(6506..6703)
                     /locus_tag="ACHATZ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404971.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6872..7450
                     /locus_tag="ACHATZ_002495"
     CDS             6872..7450
                     /locus_tag="ACHATZ_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8404972.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7545..7682
                     /locus_tag="ACHATZ_002496"
     CDS             7545..7682
                     /locus_tag="ACHATZ_002496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404973.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2500 a         1443 c         1612 g         2646 t
ORIGIN      
        1 tcacaacctc ttattttgat tagaacttgc gaaaataaac cggttaaagt gtgtctagcg
       61 caagagattt gctataatag acgataaggg taaaatgtaa cgagaagggg ttgaaaaatg
      121 tgtatcaaat tgtaacagat tcttgttgtg atctgccata ccatgtatta gcaaaagaaa
      181 aagttgattt tttatcaatg aatctccaat tgaatgatca agagttagta gatgacctcg
      241 gaaagacatt caattatgac cgctacatga atcagctgaa aaatggtgcg atgcctacga
      301 catcacaaat caatgttggc cgatatttag aatttttccg ttcttatgcg gaaaaacaga
      361 ttcctgtttt atatgtggct ttttccagtg gattaagcgg ctcttacagt agtgcgcttc
      421 aagcggttga attgctgaag gaagaatatg aagaccctga gatctatgtt tttgatacaa
      481 aagctgcaag tattggggaa gggcttttag tattggaagc tgtaagattg aaaaaagaag
      541 gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa gtccattcat
      601 gggtaacagt agacgatctg aaatatctag aacgaggcgg taggatttcc aaagcaagtg
      661 ctactcttgg cagtctattg agtgtaaagc ctattattac ggtggataaa gaaggaaggc
      721 tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat ccagacaatc gtcaatgaga
      781 cggtaaaaac aatcgttgca ccacttgatc aaatcgttta tgtggcttac gctggggatc
      841 tagagagcgc ggagaaggca aaacggcttt tggaagagca gataaagatg aaagatgtga
      901 agctttatcc cttaggacca acgatcgcca gtcacacagg ctatggctgt atcgcgattt
      961 tttcgatggg aatcagtcga taaaagcagt aactcattct gtaaaaaagc ccagaaattg
     1021 attgtaacaa aaacagcgac tcttaaaaga tagatgagca tcctatctct tagaggtcgc
     1081 tgttttttta ttttttaagc taaataggcc attatgccca taactcgttc aattgttttt
     1141 gaatatctgc attttccaaa aattcatcat atgtcgtatc agctcgatcg atcacacctt
     1201 tatccgaaac ggcaatgatg cggtttgcca atgtttggat aaattgatgg tcatgtgacg
     1261 caaataagat tgagccagta aatgccatca agccgtcatt caatgctgtg attgattcta
     1321 agtctaaatg gttcgttgga tcatctaata caagtacgtt agctttcgaa agcatcattt
     1381 ttgaaagcat gcaacggact ttctctccac cagataaaac attcactggt ttcaatactt
     1441 cttcaccaga aaacaacatt cttcctaaga agcttcttaa gaaggtgtta tcatcttctt
     1501 ctttacttgc atattgacgc aaccagtcta aaatcgttag atcagtatcg aattctttcg
     1561 ttgtatcctt tggtaaataa gaattacttg ttgtcacacc ccaacgtact gttccagtat
     1621 ctggcgtaat ttcacccatg ataactttga atagcgttgt tgttgttata tcattttctg
     1681 cgacgaaagc gaccttgtct tctttgctca aattaaaaga tatatcatct aaaattttct
     1741 ttccatcaat cgttacactt acattttcta cctgcaataa gtcgtttcca atctcacgtt
     1801 caggtttgaa tccaacgaat ggataacgtc tagaagaagg ttggatatcg tctaaagtaa
     1861 ttttttcaag cattttttta cgtgaagttg cttgttttga ttttgatgca ttcgcactaa
     1921 agcgggcaat aaattcttgg agttctttga tttgttcttc ttttttggca tttgcgtttg
     1981 cttgaagttt agccgctagt tgactagatt ccaaccaaaa atcataattt cctacataaa
     2041 gtttgatttt accaaaatct agatccgcca tatgtgtaca tactttgttc aaaaagtgac
     2101 ggtcatggga tacaacgatc acagtatttt caaaattgat caaaaattct tccagccaat
     2161 tgatcgattg gatatccaga ccgtttgttg gttcgtctag caaaagaaca tcaggtttac
     2221 caaatagtgt ttgtgctaat aacactttta ccttttgtcc accagttaat tcactcattt
     2281 gctgatggtg caattcttca gggatgttca gaccttgcag taaaacagct gcttctggtt
     2341 cagcttccca tccattaagc tcagcaaatt ctccttctaa ttcagctgct cgaataccat
     2401 cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc acttcataca
     2461 gacgcttgtg tcccatgatg actgtttcaa gtacagtata gtcttcatag tcgtaatggt
     2521 tctgtttcaa tacggccatc cgttcatctg gtcccatagt aacggaacct gtggaaggct
     2581 gcagatcccc tgataacact ttaaggaacg ttgatttacc tgcaccattt gcaccaatca
     2641 agccatagca gtttccgggg gtaaatttaa tattcacatc atcaaatagt ttgcggtctg
     2701 aaaagtgcaa actcacatca tttacagtaa tcaatttttt tcctcacttt tatcattatt
     2761 agttttgaca agtcttatag aattatatag gggaaaggta aaagattcaa ggcaatcgaa
     2821 cattctttgt gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc ggagcgaaaa
     2881 tagctactat aggatttatt agtttgcaac agcataattt ttgacgttat ttataagagg
     2941 gagtactata aaactgtaaa agaaatgaaa gaaggattga aaatggatga aaaaagaacc
     3001 atcgactggg gctcattgat tttgggaatt ttatttgtac ttgtctcatt gttatcattt
     3061 caagaccctg tcggaaattt agttgcaatt gtagtagtct ttgcgatttt tgcctttatt
     3121 aaagggatat ttgaactgtt tgtacggaac agattaaaag agctaacagg atataaagga
     3181 aagatgccct taataatcgg gatcatcgat attctagttg gcgtcttttt cttattcaat
     3241 attggtgcag gagtcgcagc attgcctttt gtttttgcag tatggtttat tgctgattca
     3301 gttttagcgt tatttactgc tgacttggca agaggagtaa gtgaaggata ttattggttt
     3361 acgatcatcg tcaatatttt aggaatttta ttaggaattt tcttattgtt taatccaatt
     3421 tcttctgcgt tgacattaag ctttttagtt ggattctatt ttatgttatt tggtatcaca
     3481 catatcgtct atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg gcttccgatt
     3541 acagttttac tgttgtaatt ggaagccggc ttttttatca tggacaaata tgcacgaggt
     3601 gatttttagg tgtcgagaca aatttatgca aaaatgtgag aagctatata cacaaatttt
     3661 atcttaatac tcgaagcata gcgcagttgt cacaacctct tttcatataa ttcattcaat
     3721 actggcaaat catggacttc atatttttct ttgaatgctt ggatttcttc ttcagaatat
     3781 tttcttggat ctaaatgcag atcttctact ggcaatgggc gcttgttatt gatcttcaca
     3841 tcgattacta caggacgatc tgattttgca gctgcatcaa aggcaaatcg aagttgcgag
     3901 tattctgtga ctgtaaagct atttgccccg agcgcttctc caactttacc gaaatctgca
     3961 tcttctaaaa atacaccaaa tttcttttgt tccgtatctt cttgttctgc ttcgatgaaa
     4021 ccaaaagaat cattcgaaaa gacaacatta ataattggaa gctgatattt gacttgtgta
     4081 atgatatctt gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa tacttgtttt
     4141 tctgggtagc ttagttgtgc ggcaattcct cctggcacac cattacccat agttgcgaac
     4201 catccagatg tcgtatgttt ttgtttaccg ttcatattca ataaacgaat agaatggata
     4261 gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg attgatttct
     4321 ttatagacag cctctggacg aatcggttct tcctctcgat cttcaaaaga tttcaaccaa
     4381 tttacccatt tttctttatt ttcttgattt gctaggtacc agctatcagc agggcgagca
     4441 tcccctaact cgacaagtct ttgcaaagct gtgtttgcat caccgaggat cgacaaagat
     4501 gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga tttggataaa ttcagcagtt
     4561 ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga caaataagat cagatcggct
     4621 tctgccaatg cttcatttcc gggtttagta gcgacacgtc cagcaaaacc aagaaagttt
     4681 tcataatcat caggaataat tccttttgct agaacggaag cgacaacagg aactgaaaaa
     4741 tgttctgaga attctttgat ttgcggaaat ccgtttcgcg ttccttgacc aacataaaga
     4801 actggttttt ttgctttttc aagataaggc aaagcagcta gcaaatcttt ttcttctggt
     4861 aatatcacac ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc aatttcttca
     4921 aaaccaagat caacagggat agtcacgata gccacgcctt tatgctcata ggcggcttta
     4981 atggcttcat ctactacatg cggaagactt tgtggtgtca tgactgtacg attgtatacg
     5041 ctaacatctg cgaaaatagg attttcattt agttcctgga aagagttata gttcatggat
     5101 gtagaagcaa cttgtcctaa gaacgctact agaggcacat ggtccatctg tgcatcatac
     5161 aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc gccgactttc
     5221 cccgttaatt ttgcatctgc agcggctgcg agtgcgccga cttcttcatg acgaacttga
     5281 atgtagttga tttcggtttt ttcttgatat agcgcatcca taattgaatt gaaagatcct
     5341 ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa gaacttttac cattgcgatt
     5401 cctgcattga tttttgacat ggtgtttcac tcctttagca atgatgatac ataaattttt
     5461 tgtatctaga gtatagaact ttttatcgaa aatcccaact aattcgtctt ttcacaaaaa
     5521 tgtagtcatg tttttgggaa tatgctagag tagtacatag atagattcaa gagaggggaa
     5581 tggcatggtt gaatggctat tgagacttga accttggcag caagcacttg ctgggacagc
     5641 ttttacttat tttatgacag ctcttggtgc cgggttggtt tttttcttta aagaaatcaa
     5701 aaaagaagtg ctaaacttga tgttaggatt tgcttctggt gtcatgattg ctgcaagttt
     5761 ttggtctttg ttagatccgg ctattacaaa agcagaagaa aatggagata ttgcttggct
     5821 agttgtaagt attgggtttg gtttaggcgg tgtgttttta tatatggcag ataaaacact
     5881 gccccatatg cattttggac cacaacatga agcagaagga ttgcctaccc atttaaaacg
     5941 gacgatcttg cttgtttttt ctattacatt gcataacatt cctgaaggtt tagccgtggg
     6001 ggtagcattc ggtgcagcag caactgcgga taatccgaca gccgctatat tagccgctgt
     6061 ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa ggagcagctg tatcaatccc
     6121 gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag cttctggaat
     6181 cgttgaacca attgctggag tgattggagc gctactagta acgaaagtgg agctgctgct
     6241 tccttatgca cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg aagaattgat
     6301 ccctgaagca cagcagacat tgtccagcaa acgtcatttt gcagtttttg gcgtcatgag
     6361 tggatttatc attatgatga ttttggatgt ggcattagga taaataaaaa aaatatatat
     6421 gatatatcaa atagggccgc gaacttatat cgcgacccta tttttttagt aagttatatc
     6481 ataatgaagt gaatggtatc atacattaat aatagttttt tctatgtgta tttcctttga
     6541 tccagcgaac aagccagatg ataagtgcga ttgggattag aactttgacc gcaaaccata
     6601 aaagcccgct taggacactg acaaaaagac tgatgacgaa gctagctgca attagcacta
     6661 aaagaaccat gaagatattt aagaacatat ttttttgacg catacaaaac cctcctcttg
     6721 ttcattcttt actatagaaa aagtataaca gggattgttt aaaaacacct ccaactgaag
     6781 actgattttt acttcatcta cttaaatgat caaacaattg ctttttctct ggtttatcat
     6841 ctaaaataga gcggaaaagg aggagaatat catgcctaaa aatcgtcttg aagcatttac
     6901 agatgctgta atcgcgatta tcatgactat tttagtttta gaacttcatg aacctaacgg
     6961 agacacgtgg gaggcgctcg ctgcattagg ttaccgattt tttatctata ttgtcagttt
     7021 tatcatttta gcgatttatt ggaacaatca tcatcattta tttcaagttg tacataagat
     7081 aaatggacgg gtcttatggg cgaacagttt ctttgttttt gcgctttcgc tttttccgtt
     7141 tgcaacttca tggatcagtg agtacatcaa tagcctggct ccagaaatga cttatggttt
     7201 tgttaccttg accgctaata ttgcgtatta tatcttggca aaagaacttg tcaaagcaaa
     7261 taaaggccac gagaaagcat tgaaggcgat gttgaataat tacaagaaat cgtatctatc
     7321 gattggtttg aatgtgttag gaattgtctt agggtattta gtagcgccag tagccgtgct
     7381 gatcgcaaac atcgtgattc tagctgcttg gatcatccca agtcgtacca tcgaatcaca
     7441 atatcggtaa aatgatccgt gtttaaaatt taagagaaaa atatagaaaa aataaataaa
     7501 cgatcaaata tccgagtgat actagtattc aaagagggat gcagatggat caaaaaagaa
     7561 ttcatgtagg actgattact agttttgtca cgatgcttat ttatttggat caaggtcatt
     7621 tgacttggac agtagttggt gtcgtaggat ttttgacatt attcacagca ataaaaaaat
     7681 aaacatcttt atgtaatctg atgttctatc tatttaagaa aagcatgttt acagcagtat
     7741 gattctgttg taaacgtgtt tttatttgtg gaaaatttaa tttttagaaa agtgtatcga
     7801 aaaatagata gttagttttt ccacagtgga taacttcgta agagataaaa ataaaaaatt
     7861 agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa atgaataaat
     7921 attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa aataacatat
     7981 ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa ataccgtgtc
     8041 tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg ccttcaaaaa
     8101 gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa taaggagaaa
     8161 aaccatagaa aatataaagt aattacttat aaaacaagct t
//
LOCUS       DAYYAJ010000126         8143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000126 DAYYAJ010000000
VERSION     DAYYAJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.126"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACHATZ_002497"
     CDS             382..570
                     /locus_tag="ACHATZ_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8404974.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACHATZ_002498"
     CDS             648..1661
                     /locus_tag="ACHATZ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8404975.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACHATZ_002499"
     CDS             complement(1728..2762)
                     /locus_tag="ACHATZ_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8404976.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACHATZ_002500"
     CDS             2877..5102
                     /locus_tag="ACHATZ_002500"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8404977.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACHATZ_002501"
     CDS             5120..7003
                     /locus_tag="ACHATZ_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8404978.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACHATZ_002502"
     CDS             6984..7958
                     /locus_tag="ACHATZ_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8404979.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2638 a         1480 c         1712 g         2313 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc
     8101 tgaaaaacaa agttaactat cagaaaatta aaaaaagttt cat
//
LOCUS       DAYYAJ010000127         7882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000127 DAYYAJ010000000
VERSION     DAYYAJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.127"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACHATZ_002503"
     CDS             complement(220..972)
                     /locus_tag="ACHATZ_002503"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8404980.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACHATZ_002504"
     CDS             1085..2005
                     /locus_tag="ACHATZ_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8404981.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACHATZ_002505"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACHATZ_002505"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8404982.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACHATZ_002506"
     CDS             3311..4789
                     /locus_tag="ACHATZ_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8404983.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="ACHATZ_002507"
     CDS             5066..7126
                     /locus_tag="ACHATZ_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8404984.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="ACHATZ_002508"
     CDS             7209..7778
                     /locus_tag="ACHATZ_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8404985.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         2803 a         1319 c         1506 g         2254 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg cc
//
LOCUS       DAYYAJ010000128         7796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000128 DAYYAJ010000000
VERSION     DAYYAJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.128"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..1368)
                     /gene="celB"
                     /locus_tag="ACHATZ_002509"
     CDS             complement(16..1368)
                     /gene="celB"
                     /locus_tag="ACHATZ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8404986.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1368..1886)
                     /locus_tag="ACHATZ_002510"
     CDS             complement(1368..1886)
                     /locus_tag="ACHATZ_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404987.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1902..2228)
                     /locus_tag="ACHATZ_002511"
     CDS             complement(1902..2228)
                     /locus_tag="ACHATZ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8404988.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2241..4220)
                     /locus_tag="ACHATZ_002512"
     CDS             complement(2241..4220)
                     /locus_tag="ACHATZ_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8404989.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4241..4555)
                     /locus_tag="ACHATZ_002513"
     CDS             complement(4241..4555)
                     /locus_tag="ACHATZ_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8404990.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4723..5016
                     /locus_tag="ACHATZ_002514"
     CDS             4723..5016
                     /locus_tag="ACHATZ_002514"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8404991.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5094..6206)
                     /locus_tag="ACHATZ_002515"
     CDS             complement(5094..6206)
                     /locus_tag="ACHATZ_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8404992.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6359..7207
                     /locus_tag="ACHATZ_002516"
                     /pseudo
     CDS             6359..7207
                     /locus_tag="ACHATZ_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7560..>7796)
                     /locus_tag="ACHATZ_002517"
     CDS             complement(7560..>7796)
                     /locus_tag="ACHATZ_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8404993.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2493 a         1551 c         1260 g         2492 t
ORIGIN      
        1 cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct tttctctttc
       61 ttcatttaat aatttattat catagaattt gataaatggg aaccagataa tgaatgcgat
      121 gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac caacaaatgc
      181 tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga cgatttccag
      241 ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa acggaaccgc
      301 taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga tattgaataa
      361 tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg cgaggaatgc
      421 aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat aagaattgtt
      481 gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat tagctaatac
      541 gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga tccacaatgc
      601 atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact gcgtaacaaa
      661 cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta ataatccatt
      721 gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga aagaacgaga
      781 aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga cacacataca
      841 atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg aagttcctaa
      901 acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct cattgataga
      961 agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca taaagaatgc
     1021 aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt catctcgttc
     1081 aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa tccccatcgt
     1141 cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat taaaaaatcc
     1201 ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg agcctacaat
     1261 cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt taagttgcgc
     1321 aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta gttattctcc
     1381 tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg gtcttggttg
     1441 agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc gcttacaaca
     1501 agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata caagaatgta
     1561 aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg gaacaaaaaa
     1621 taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac ttgttctcct
     1681 actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa acttttagct
     1741 gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt caggtaacgt
     1801 acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt ttctaattcg
     1861 attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt tttttgatac
     1921 aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag atgatcttgt
     1981 gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc tttcagaagt
     2041 tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa cttctcttct
     2101 acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag tgtccgtgca
     2161 tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact gtttattttt
     2221 tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct gaaattctca
     2281 aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa cctaacgatt
     2341 gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac taaaaaaaca
     2401 aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc aacggctacc
     2461 tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc actgaatatg
     2521 atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat atatccttct
     2581 tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt tttcggttcg
     2641 tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg ctttttttgc
     2701 tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga ttctccaact
     2761 acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg taaaactgga
     2821 attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat tccttttaaa
     2881 gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt tttaccgaat
     2941 tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc acagataatc
     3001 aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag atgcaaagcc
     3061 aaataggccc attcatcttc attgatggta tagtttttca aagccggcat gttgctgaaa
     3121 tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc agtcaatggg
     3181 ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg atcgagtagt
     3241 ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga caatcttttc
     3301 agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt gtgattcgac
     3361 ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga tacacctgcg
     3421 attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg cttagaaact
     3481 tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt gatccccaaa
     3541 gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt agcttctctt
     3601 gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa aaaacggctt
     3661 tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt atccatgata
     3721 aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg cttagaaacg
     3781 atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt tgtcaaactg
     3841 gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc atcttccaaa
     3901 agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc ttgcggttct
     3961 ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt cagtctttgt
     4021 aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc gtttttttct
     4081 actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa taacaattca
     4141 gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata tctcaacagc
     4201 aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc tcttcgatca
     4261 cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa gggatactaa
     4321 caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac atcgtcgttt
     4381 gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg ccttctgtag
     4441 ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc gttgtctttg
     4501 aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt ttcataaatc
     4561 aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct attcaaaata
     4621 tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata atttctggca
     4681 ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat cacagtgaat
     4741 attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat ggtcgaaaga
     4801 aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta ttactttcca
     4861 ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga agcaatcgaa
     4921 aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa gtataaatta
     4981 aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg aatgatagca
     5041 atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct catctaatga
     5101 ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc aatatacatg
     5161 attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc tcctggaacc
     5221 ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt tttatatcta
     5281 taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt aaaaggaata
     5341 aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat tcggtctact
     5401 gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga agaaaatgca
     5461 aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat caaaagacta
     5521 atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc tttcaaaaaa
     5581 atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta aaggagaact
     5641 gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa attcttgttc
     5701 aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta caaagaagca
     5761 ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa aataaaatga
     5821 atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac taatagtgag
     5881 aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa gtcaacaaaa
     5941 cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt cgattcaaat
     6001 aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa tgatcgtaaa
     6061 aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg tattttttgt
     6121 tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg tcgtcctttc
     6181 tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg gaacatcata
     6241 tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa aaaaacatat
     6301 cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga tatgctttat
     6361 gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga ttacagaggt
     6421 ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc tcttccctgc
     6481 tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga aattaatggt
     6541 ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc atatgccaat
     6601 ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc attggaacgc
     6661 tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac ataaaatcac
     6721 ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt aggtatcttt
     6781 gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt cttccaaaag
     6841 aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt tgaggataaa
     6901 ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat ttacctacac
     6961 aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa gaaatagaat
     7021 tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt ctaccaaatg
     7081 cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag gcgtaacctt
     7141 tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt tctccttatt
     7201 gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta tttgtagaaa
     7261 gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact gcgaaactca
     7321 agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact acaaaggttt
     7381 atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt tgggttcaac
     7441 cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg tagtttccat
     7501 tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt tattttagct
     7561 catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac acccaatatt
     7621 gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt atttccactg
     7681 cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt ctcttttgtt
     7741 aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt cgcatc
//
LOCUS       DAYYAJ010000129         7675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000129 DAYYAJ010000000
VERSION     DAYYAJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.129"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACHATZ_002518"
     CDS             <1..106
                     /locus_tag="ACHATZ_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8404994.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(175..903)
                     /locus_tag="ACHATZ_002519"
     CDS             complement(175..903)
                     /locus_tag="ACHATZ_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8404995.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(916..2196)
                     /locus_tag="ACHATZ_002520"
     CDS             complement(916..2196)
                     /locus_tag="ACHATZ_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8404996.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2337..2939)
                     /locus_tag="ACHATZ_002521"
     CDS             complement(2337..2939)
                     /locus_tag="ACHATZ_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8404997.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3184..3390
                     /locus_tag="ACHATZ_002522"
     CDS             3184..3390
                     /locus_tag="ACHATZ_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8404998.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3702..4421
                     /locus_tag="ACHATZ_002523"
     CDS             3702..4421
                     /locus_tag="ACHATZ_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8404999.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4502..5185)
                     /locus_tag="ACHATZ_002524"
     CDS             complement(4502..5185)
                     /locus_tag="ACHATZ_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8405000.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5404..7590
                     /locus_tag="ACHATZ_002525"
     CDS             5404..7590
                     /locus_tag="ACHATZ_002525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8405001.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2758 a         1293 c         1446 g         2178 t
ORIGIN      
        1 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
       61 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
      121 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
      181 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
      241 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
      301 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
      361 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
      421 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
      481 tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt
      541 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
      601 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
      661 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
      721 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
      781 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
      841 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
      901 catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc
      961 aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa
     1021 gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt
     1081 taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt
     1141 gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc
     1201 caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg
     1261 ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga
     1321 cagtaaacta cggatctcag gaactttcat attatccaac atacttagat tggccggaaa
     1381 aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc
     1441 taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt
     1501 gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct
     1561 gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc aataataaac
     1621 caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac
     1681 atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat
     1741 caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga
     1801 tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata
     1861 ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct
     1921 tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg
     1981 ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc
     2041 agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac
     2101 tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta
     2161 aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt
     2221 accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag
     2281 aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa
     2341 tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc
     2401 aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag
     2461 aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg
     2521 ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt
     2581 aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga
     2641 tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat
     2701 tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg
     2761 attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt
     2821 ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct
     2881 tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc
     2941 tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca
     3001 taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa
     3061 gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt
     3121 gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt
     3181 cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta
     3241 tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt
     3301 gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt
     3361 gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag
     3421 gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca
     3481 tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa
     3541 aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg
     3601 gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa
     3661 atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca
     3721 ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt
     3781 tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt
     3841 ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg
     3901 caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata
     3961 gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt
     4021 atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac
     4081 cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa
     4141 gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac
     4201 tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg
     4261 atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa
     4321 tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta
     4381 caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt
     4441 gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag
     4501 atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg
     4561 ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac
     4621 agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa
     4681 atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc
     4741 cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag
     4801 ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat
     4861 ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc
     4921 gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc
     4981 ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa
     5041 atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt
     5101 ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac
     5161 gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc
     5221 cttatcttag cataaacgat tgaaaaaaaa ggaaaaattt tcagaaaact aaaatcatat
     5281 cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata gacaacgaaa
     5341 ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat
     5401 cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag
     5461 tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg
     5521 gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga
     5581 aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa
     5641 aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc
     5701 ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac
     5761 gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga
     5821 gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt
     5881 tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa
     5941 ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt
     6001 aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac
     6061 tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat
     6121 gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg
     6181 gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat
     6241 ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat
     6301 ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg
     6361 acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga
     6421 ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact
     6481 cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg
     6541 atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca
     6601 ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa
     6661 caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat attataccga
     6721 tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta
     6781 ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat
     6841 caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag
     6901 caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac
     6961 ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat
     7021 ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag
     7081 catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa
     7141 atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga
     7201 agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg
     7261 attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa
     7321 ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac
     7381 ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtaacgacg
     7441 atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat
     7501 ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa
     7561 accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag
     7621 tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatt
//
LOCUS       DAYYAJ010000130         7613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000130 DAYYAJ010000000
VERSION     DAYYAJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.130"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACHATZ_002526"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACHATZ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACHATZ_002527"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACHATZ_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACHATZ_002528"
     CDS             complement(1147..1716)
                     /locus_tag="ACHATZ_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8405002.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACHATZ_002529"
     CDS             complement(1790..3079)
                     /locus_tag="ACHATZ_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405003.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACHATZ_002530"
     CDS             3406..4281
                     /locus_tag="ACHATZ_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8405004.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACHATZ_002531"
     CDS             4295..4618
                     /locus_tag="ACHATZ_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405005.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACHATZ_002532"
     CDS             4615..5367
                     /locus_tag="ACHATZ_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8405006.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACHATZ_002533"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACHATZ_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2268 a         1507 c         1376 g         2462 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acg
//
LOCUS       DAYYAJ010000131         7465 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000131 DAYYAJ010000000
VERSION     DAYYAJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.131"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..3693
                     /gene="rpoB"
                     /locus_tag="ACHATZ_002534"
     CDS             76..3693
                     /gene="rpoB"
                     /locus_tag="ACHATZ_002534"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8405007.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            3772..7425
                     /gene="rpoC"
                     /locus_tag="ACHATZ_002535"
     CDS             3772..7425
                     /gene="rpoC"
                     /locus_tag="ACHATZ_002535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8405008.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
BASE COUNT         2379 a         1496 c         1692 g         1898 t
ORIGIN      
        1 aaataatatt tgaaatatta ttgtaacaat tgatcgggta gtctttttga atgacatttt
       61 agaggggtga agagcttggc tggacacgta gtaaaatacg gaaaacatcg cgaacgtaga
      121 agtttcgcaa gaatcagtga agtgttggaa ttaccaaatt tgatcgaaat tcaaacagac
      181 tcttaccaat ggttcttaga tgagggactt agagaaatgt ttgaagacat tttgccaatc
      241 gatgacttca atggcaatct atctttggaa tttgtagatt atgaattgaa agaaccaaag
      301 tatacagtag cagaagctcg tgcgcatgat gcaaactact ctgcaccatt acatgtaaca
      361 ttgcgtttga caaaccgcga gactggcgaa atcaaagcac aagaagtatt cttcggtgat
      421 ttcccattaa tgacagaaca aggaacattc atcatcaacg gggcagagcg agtaatcgtt
      481 tctcagttag tccgttcacc aggtgtctat ttccatggaa aagtcgacaa aaacggaaaa
      541 gaaggattcg gttctactgt tatccctaac cgtggagctt ggctagaaat ggaaactgat
      601 gcaaaagaca tctcttatgt acgtatcgac cgtacacgta agattccttt aaccgtatta
      661 gttcgtgcgt taggatttgg ttccgatgat acgatctttg aaatcttcgg cgacagcgaa
      721 acattacgca atacagtcga aaaagacttg cataaaaacg caagcgactc aagaacagaa
      781 gaaggtttga aagacgttta cgaacgtttg cgtccaggcg aaccaaaaac agcagacagc
      841 tcacgtaact tgctgaatgc tcgtttcttc gatccaaaac gttacgactt agcaaacgtt
      901 ggtcgttaca aagtaaacaa aaaattagat ttgaaaacac gtctattgaa tctgacatta
      961 gctgaaacat tagttgatcc agaaacaggc gagatcatcg ttgaaaaagg aacagttctg
     1021 actcaccaag tcatggaaac actagctcct ttcattgata atggcctaaa ttcagtgact
     1081 tactatccat cagaagacgg tgtggtcaca gatccaatga ctgtccaagt catcaaagta
     1141 ttttcaccaa gagaccctga acgtgaagta aacgtgatcg gaaacggcta tccagaagca
     1201 gctgtaaaaa cggttcgtcc agccgacatc attgcttcaa tgagctattt cttgaacttg
     1261 atggaaggta tcggcaatgt cgatgatatc gaccacttag gaaatcgtcg tatccgttca
     1321 gtcggtgaac ttttacaaaa ccaattccgt atcgggctag cacgtatgga acgtgtggtt
     1381 cgtgaaagaa tgtcgatcca agatactgaa acattgacac ctcaacaatt gatcaatatc
     1441 cgtccagttg tggcaagtat caaagaattc tttggttctt ctcagttgtc acagttcatg
     1501 gatcaaacaa accctcttgg cgaattgaca cacaaacgtc gtctttcagc cttaggacct
     1561 ggcggattga ctcgtgaccg cgccggttat gaagttcgtg acgttcacta ctcccactat
     1621 ggccgtatgt gtccgatcga aactcctgaa ggcccaaaca tcgggttgat caatagtttg
     1681 tccagttatg cgaaagtaaa taaattcgga tttatcgaaa caccttatcg tcgtgtagac
     1741 cgtgaaacag gtcgtgttac tgaccaaatc gactacttga cagctgatat cgaagaccat
     1801 tacatcgtag cccaagcaaa cagcccattg aatgaagatg gaacatttgc tcaagacgtt
     1861 gttatggctc gtgctcaaag tgaaaactta gaagtatcta ttgataaagt agactacatg
     1921 gacgtatcac ctaaacaagt agttgccgtt gcgacagcat gtattccttt cttggaaaac
     1981 gatgactcca accgtgcctt gatgggtgcc aacatgcagc gtcaagcagt gccgttgatc
     2041 aatcctcaag caccatgggt tggtactggt atggaataca aatcagcgca tgactcagga
     2101 gctgctttgt tatgtaaaca tgacggtgtc gttgaatacg ttgatgcttc tgaaatccgc
     2161 gtacgccgcg acaacggtgc attagataaa tacgatgtaa caaaattccg tcgttcaaac
     2221 tccggaacaa gctacaacca acgtccaatc gttcatttgg gtgagaaagt cgaaaaaggg
     2281 gatactctgg cagacggacc atctatggaa caaggcgaaa tggctctagg ccaaaacgtt
     2341 ttagttggtt tcatgacttg ggaagggtac aactatgaag atgcgatcat catgagccgt
     2401 cgtttggtaa aagatgacgt atacacttct atccatattg aagaatatga atcagaagcc
     2461 cgcgatacaa aattaggacc tgaagaaatc acacgcgaaa tccctaatgt cggggaagac
     2521 gcattaaaag atttagacga aatgggtatc atccgtattg gtgcagaagt ccaagatggc
     2581 gatttgcttg tagggaaagt cacgccaaaa ggggtaacag aattatctgc agaagaacgt
     2641 ctattgcatg cgatctttgg cgaaaaagct cgtgaagtcc gcgatacatc attacgtgta
     2701 ccacatggcg gcggcggtat cgttcacgat gtgaaaatct ttactcgtga agctggagat
     2761 gaattgtcac caggcgtaaa catgttagtt cgtgtttata tcgttcaaaa acgtaaaatc
     2821 catgaagggg ataaaatggc cggtcgtcac ggaaataaag gggttgtatc ccgtattatg
     2881 ccggaagaag atatgccatt cctacctgat ggaacaccaa tcgatatcat gctgaatcct
     2941 ttaggggtgc cttcacgtat gaacatcggg caagtattgg aattacactt gggtatggct
     3001 gcacgtcagt taggtatcca cgtagcaacg ccagtatttg atggtgcaag tgatgaagac
     3061 gtatgggaaa cagttcgtga agccggtatg gccagcgatg cgaaaactgt tctgtacgac
     3121 ggacgtacag gtgaaccatt tgatggacgt gtgtctgtcg gtgtgatgta catgatcaaa
     3181 cttgctcaca tggtcgatga caaattgcat gcacgttcaa tcggacctta ctcattggtt
     3241 acacaacaac cattgggagg taaagctcaa tttggtggac aacgttttgg tgaaatggaa
     3301 gtatgggcac tggaagctta cggtgcggct tatactttac aagaaatctt gacatacaaa
     3361 tcagatgacg tagtcggacg tgtgaaaaca tacgaagcaa tcgtcaaggg tgaaccaatt
     3421 ccaaaaccag gtgtaccgga atcattccgc gtattggtga aagaattgca atcactaggt
     3481 ctggacatgc gtgtcttgga tatcgaagaa acagagatcg aacttcgcga catggacgat
     3541 gaagacgacg atttgatcac tgttgatgct ttgacaaaat ttgccgaaca gcaaactgcc
     3601 aaagaattag aaaagaaagc tgctgaacaa gtggaagatg aaaaagacga tgtcatccaa
     3661 aactttgaaa ccgcagaaga taatttagac taatccggtc tatgagctgc aaggcggtcg
     3721 aagatcagcc gtcttgcggt ttgccattaa atgaaatgga gggaacccct tttgatcgat
     3781 gtaaataaat tcgaaagtat gcaaataggt ttggcttctc ccgaaaaaat cagaagctgg
     3841 tcttacggtg aagtaaaaaa acctgagacg attaactatc gtaccttaaa acctgaacgc
     3901 gaagggttgt tctgtgagcg gatttttggt cctacaaaag actgggaatg tgcctgcgga
     3961 aaatacaaac gtatccgtta taaaggaatc gtttgtgatc gatgcggagt ggaagttact
     4021 cgttcgaaag ttcgccgtga acgcatggga catattgaat tagcagctcc tgtttcacat
     4081 atctggtact ttaaagggat tccttcacgt atgggtcttg tattagacat gagcccacgc
     4141 gctttggaag aaatcattta ttttgcttca tatgtagtca tcgaaccagg aaatacgaat
     4201 ttagagaaaa aacaattgtt gacagaacgc gaatatcgcg aaaaacgtga acaatatgga
     4261 caagaattcc aagctgctat gggtgcagaa gcgattaaac aattacttga taatgttgat
     4321 ctagatgcag aagcagccga attgaaagaa gaattgaaat ctgcacaagg acaaaaacgt
     4381 acacgtgcaa tccgccgttt ggatatttta gaagcattcc gcgcttcagg aaatcaacca
     4441 agctggatgg ttatggacgt tatccctgtt attccaccag atttacgccc aatggttcaa
     4501 ttagaaggtg gacgttttgc aacaagtgac ttgaatgatc tttaccgtcg tgtaatcaac
     4561 cgtaataacc gtttgaaacg tttgttagac ttgaatgcac ctggaatcat cgttcaaaat
     4621 gaaaaacgta tgctgcaaga agcagtagat gcattgatcg ataacggtcg tcgcggacgt
     4681 ccagttactg gaccaggtaa ccgcccattg aaatctcttt ctcatatgtt gaaagggaaa
     4741 caaggtcgtt tccgtcaaaa cttgctaggt aaacgtgtcg actactcagg tcgttccgtt
     4801 atcgttgttg gacctttctt gaagatgtac caatgtggac ttccaaaaga aatggccatt
     4861 gaattattca aaccattcgt gatgcgtgag cttgttcaac gcgaactagc ttcaaacatc
     4921 aaaaatgcga aacgtaaaat cgaacgtcaa gaagacgaag tatgggatgt tttagaagac
     4981 gtaatcaagg aacacccagt attgctgaac cgggcaccta cgcttcaccg acttggtatc
     5041 caagcattcg aaccagtttt agtacaagga cgcgcaatcc gtttgcatcc acttgtatgt
     5101 gaagcttata atgccgattt cgatggagac caaatggctg tccacgttcc attgaatgaa
     5161 gaagcacaag cagaagcacg tatgttgatg ttagctgctc aaaacatctt gaaccctaaa
     5221 gatggtaaac cagttgttac tccttcccaa gatatggttc taggtaacta ctacttgaca
     5281 atggaagaag aaggacgcga aggcgaagga atggtcttca gagatcaaaa cgaagccgtt
     5341 atcgcatggc gcaacggata tgttcattta cattcacgta tcggtgtgaa ccctaactca
     5401 ttaggtgaaa aaccatttac tgaatggcaa aaagaacgta cgatgatcac aacagttggt
     5461 aagatcatct tcaacgagat catgcctcct gaattccctt atctgaatga accaacagac
     5521 ttcaacttga ccgtccaaac accggacaaa tactttgttg aagctggtac tgatattcct
     5581 gcacatatca aagagcaaga attagtatta ccgttcaaga agaaaaactt aggaaacatc
     5641 atcgcagaag tcttcaaacg attcaaagta acagaaactt ctaagatgct tgaccgtatg
     5701 aaagacttag ggtacaaaca ttcgacacat gccggtatca cagtagggat cgctgacatc
     5761 agtgtactga atgaaaaaca agaaatcatt gaaaatgccc ataaacaagt tgaaactatt
     5821 acaaaacaat tccgtcgtgg tctgatcaca gatgacgaac gttatgaacg tgtcattgct
     5881 gtttggaatg ctgcaaaaga cagcatccaa caaaaactga tggaaggttt ggaagcgaaa
     5941 aatccaatct tcatgatgtc tgattctgga gctcgtggta acatttccaa ctttacacag
     6001 cttgccggaa tgcgtggttt gatggccgct cctaatggtc gaatcatgga attgccaatc
     6061 atttcgaact tccgtgaagg actatccgtc ttggaaatgt ttatctcaac tcacggggct
     6121 cgtaaaggga tgaccgatac agccttgaag actgccgatt caggttactt gactcgtcgt
     6181 ctggttgacg tggcccaaga tgtaatcatc cgtgaagaag attgcggtac tgaccgaggt
     6241 cttgaaatcc aagccatccg cgagggaaat gaaatcatcg aatcattaga agatcgttta
     6301 gtgggacgtt acacccgtaa agaagtacgt catcctgaaa caaatgaatt gatcattgca
     6361 ggaaaccaat tgatttccga agacatcgca aaacaaatcg ttgatgcagg tgtcgaaaca
     6421 gtgacaatcc gttctgtctt cacatgtaat acaaaacatg gtgtctgcaa acattgttac
     6481 ggacgcaacc ttgcaactgg ctctgacgtt gaagtcggag aagcagttgg tacgatcgcc
     6541 gctcaatcaa tcggtgaacc tggtactcag ttgacgatgc gtacattcca tacaggtggg
     6601 gttgccggag acgatatcac tcaaggtctt cctcgtgtcc aagaaatctt tgaagcacgt
     6661 aatccgaaag ggcaagcagt gatcacagaa gtaacagggg atgtcatcga tatctcagaa
     6721 gatcctgcaa cacgtaccaa agaagtaaca atcaaaggaa aaacagatac acgtacgtat
     6781 actgttcctt atacagcacg tatgaaagta gcagaaggcg atatgatcca ccgtggtgca
     6841 ccattgacag aaggatcaat cgatccaaaa caattgttgc aagttcgcga tgtattatct
     6901 gttgaaaact acttgcttcg tgaagtacaa cgtgtttacc gtatgcaagg ggtagaaatc
     6961 ggcgacaagc atatcgaagt aatggttcgt caaatgctgc gtaaagttcg tgtaatggat
     7021 ccaggtgata cagatatctt accaggtaca ttattagaca tcgcagactt taaagatcaa
     7081 aactacaata cgctagtctc aggaggcgtt cctgcaactt ctcgacctgt cttactaggt
     7141 atcacaaaag catcattaga aacaaatagt ttcttatctg ctgcatcatt ccaagaaaca
     7201 actcgtgtct tgacggatgc tgcgatccgc ggtaagaaag atcctcttct tggtttgaaa
     7261 gaaaatgtta ttataggtaa gatcatccca gctggtactg gtatgcaacg ttatcgtgac
     7321 atggaaccaa aagaagtcgg tgtagcaagc gaaaatgtct acagtatttc agatattgaa
     7381 gcgcaaatgg ctgcagaaga cgcaatgaac aatcaaacaa aatagatatt ttaaaaaaga
     7441 ggttgtgaca aattattgtc acaac
//
LOCUS       DAYYAJ010000132         7413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000132 DAYYAJ010000000
VERSION     DAYYAJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.132"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..900
                     /locus_tag="ACHATZ_002536"
     CDS             52..900
                     /locus_tag="ACHATZ_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8405009.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1546..1752
                     /locus_tag="ACHATZ_002537"
     CDS             1546..1752
                     /locus_tag="ACHATZ_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8405010.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1954..2955)
                     /locus_tag="ACHATZ_002538"
     CDS             complement(1954..2955)
                     /locus_tag="ACHATZ_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8405011.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2960..4873)
                     /locus_tag="ACHATZ_002539"
     CDS             complement(2960..4873)
                     /locus_tag="ACHATZ_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8405012.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5041..5547)
                     /locus_tag="ACHATZ_002540"
     CDS             complement(5041..5547)
                     /locus_tag="ACHATZ_002540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8405013.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5707..6147
                     /locus_tag="ACHATZ_002541"
     CDS             5707..6147
                     /locus_tag="ACHATZ_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8405014.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6173..7330)
                     /locus_tag="ACHATZ_002542"
     CDS             complement(6173..7330)
                     /locus_tag="ACHATZ_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405015.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT         2153 a         1426 c         1149 g         2685 t
ORIGIN      
        1 ttaatgagaa cggaaaaaat gataatctta ttatgaaaat gaggaattct catgagcgtc
       61 tccataagac tacaaatcaa aacaatgtac aacgacttca gcccaaaaga acaggctatc
      121 gccgattata ttttagaaaa tccaagcaaa gtaagtcata gtcctattag caatttagca
      181 agtgaactgg gcattgctga ctctactttt tttcaattta caaaaaaact tggttataat
      241 ggatttaaag attttaaaat ggccatgttg atccaagaaa atgatctatc tgctatttct
      301 atccacgaaa atattcagaa aacagatact gagctcacta tggcgcaaaa ggtgttcgag
      361 tctagtattt caactttaaa tgacacaaaa aagttgttac aacaaaagga tctaaaaaaa
      421 gcggctacta ttatcaatga atctaaacgt gtctattttt tcggtgttgg cggttcagag
      481 attgtagcaa ctgacgctta tcataaattt ttgcgttccc ctatcgcaac ttctcacagc
      541 acggattacc atattcaatt gatggaagct tctttattga cggaaaaaga ctgtgctgtt
      601 ttgatttctc acacagggca atcaaaagaa acaatccata ttgctgaaac cgtaagaaaa
      661 actggagcaa aaacaatcgt tattactagc caagctaatt cttcccttgc taaacttggc
      721 gatgtcgttt ttatttctat ttctgaagaa acagaattcc gatctgaagc tttagcttcc
      781 cgtatttctc aactaagcat tttggactct ctatatgtga ttttgatgtt ttacaacgaa
      841 aaagaatcac gtcgttctat cgctaaagtt cgacatacta tttctgaaat caaagaatag
      901 caccataaca gaaaaagcga aaaatccata aaacaaaaaa agccactacc aaatatttgg
      961 tagtggcttt tttggttctg caagggtggt acagaacatc aatagtcggt gggttagaac
     1021 gtatgtcatc gcatctttgg agaaggttgt attggtatta ttcgagttac ttgttagatg
     1081 cgataacaaa gttagtatac agtagtaaaa aatttatctt ttcttttttt tattattttt
     1141 gtttcttatt ttctaaaaaa ttaaaaaaaa ttttggtgaa ttaagtattt ccttgataac
     1201 actggctttt ggcctcattt taggttatca tttgtaattt tatttcacag ttgatatgat
     1261 tgttatgtaa aataaaaaag tttaggatac atcgaatttt tggggaaatt cttttggggg
     1321 tatcctaaat cttttctatt atattcccat attttctatt attggtctag acgtatgatg
     1381 gtaaactttg tacctatctg attgctgaat cattaattgc ttatcattag ccatctttgt
     1441 ttcaagttcg ctagatgatc attttattca catcctaaca aacttgtcac atttatagat
     1501 tcttaatcta tttatcatac aattataaaa aaggaggtgc tgcttatgaa agctatccat
     1561 atttctttgc tttttttcgc tagtgcattg attttatttg ctattcatct ttgttcccag
     1621 ccttatatca ataacaacgg aatattagtg gaaccagcat tcttttgctt accactcggc
     1681 tttttgtccc ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa
     1741 caacaaatct gacttataaa atttacgtaa aagagatcgt gacaataatc gtgtgatatt
     1801 cccaaatttc gaataaaagg tgccccttgc catctctaac tcctcagtca caagttacaa
     1861 tattcataaa ccatgaaaag cttgtttatt gaaattgctc cttgtgactt ggagctaagt
     1921 gacagattac aacctctttt gcatcataag actttactcc ttgaaaaaca ttactaactt
     1981 ttgatatgtt tcttgcgctt gtttattgct gaaatcaaac tgatattcat ggttcaaaac
     2041 agcatttttc tccgcataat ctacttttgt gactttcacc cccgctttct caaatacaga
     2101 agttacttct tccatttgtt tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt
     2161 ttgcgtccaa cgatccgcat gtctgaccaa ggctaaactt ttgaccttct ctgattgttc
     2221 aaagtcctta ttatcaaagt aagcccatcc acatttttca taaagaaagc tagagatttt
     2281 atcacttact tgagtaagtg cagaaggatc caacagacca gacaaagaaa taaacttcgt
     2341 aatctgcttg ttctccaaag tcggatcaag atgatcagct tttttaattt cttcattttg
     2401 aagtgctaac aaatattctc cactaatttg tgcacctgcc gaatttccac caatcgaaat
     2461 attcgaccaa tctgcttgat cttttatctc ttttttagct gcttgaatgg cttcgttcaa
     2521 ttggataact ggtgtaggat gttttgcttc cggtgatcgt tcataatcta tatttacgat
     2581 gatttgctgc gtgtctgaac atagatattc caaataatca cgacaatcat ttttatcccc
     2641 tcctacatat gcgcctccat gcatccagaa aagaatcggt aattttttct tttccacttt
     2701 cggtgtgtag atatccatat tattattttt gtactttgaa ggataggata tttctttttt
     2761 tatatcgatt tgttcgattt tttgctggaa atcatctggt cgggcatata cttgttcttt
     2821 ttcacttgtg tcaaattgtt ttctaacaag caagctaatc ggctttggtg tgatttgatt
     2881 aatcaaaaat acagtgacta aaataactac gatgataact ggtactgcaa tcactattcg
     2941 cttgaaccat ttcattcatt taccctctct ttcttcgcca aatccatgtg ataatcgtgc
     3001 taacagataa aaatccacct gaataaatga gaagcgaatg tttcttttct ccagttttcg
     3061 gcaacattct tttttttgca tctgtctttt tttgtacttt cactgtcatc tgaatcggtt
     3121 gcgtatcatc acttgattga tcctctcgag gttcattctt tcccttattg tctgcattct
     3181 ctgtcggatc ttgctttttt ggtggagcta ctgggatttt tttgtaatag aaaataatag
     3241 ttggtatcgt ctcattaaaa atcccttcag tcggtccttc aacatttgct aattcgtatc
     3301 cttcgaatgt cttttgctct gtcatataat tttctccata agctccgtaa agctggattt
     3361 cgtcctggtc tttgagtaga cttttattgt tttcatccac atactttacg tttatctgat
     3421 catggaacac tactttcaaa aagtccgtta catgtttcgt gtaggcagca tggtcaactt
     3481 cctgtgcctt tccatgccct gctccttcta ctaataaaag atctttatac cctcctgata
     3541 actcatataa tttttgcgcg ttagacacag gtactacatc atctgctgtt ccatggataa
     3601 tcaatttagg caatgaatct gacattccaa tcgtaggtag agcagctttt gtatccaaat
     3661 gcatctcctg atcaaaatag tattgtttca aaaggcccat aaataaatct tcatgctctt
     3721 gatccccaat gatgtctaac tgatttttta tggcatccgg tagtgcgggg gctactgtct
     3781 ggctgtacgt aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc
     3841 ctttgacatt gacaggaata tctttcgcta atacagacat catcgtagct gctcccatcg
     3901 aaccaccata aagtaaaatc ttttgagatg gccgctctcg aacttcttga tttatccagt
     3961 aaaggacgtc atctgactca tattgtccat aggtcacata atcgcctccg ctattaccag
     4021 tcgctctgga atcaacaaat aataaattat acccggcctt ataaaaatca ttgtattctt
     4081 ctgtaacgat cccgttattc caattccctc gaaatccgcc atggatcatg actgttttat
     4141 ctgaaccttg atcgacataa tatgcatata catccatcat tttgccatct tgtttgttta
     4201 gtacagtttg atgaagttgt tttgtatttg gatagaagcc tgtgttttct gtaggcgttg
     4261 tcccatcatc tgtacctcca agcatcgaca tcaacacact gttttttgca aaagcgatat
     4321 cgtacaatag ataagtagca atcagatgtt tgataatctc tttatctaca ccataatcat
     4381 taacagcaag ctcagtgact aataactcta ttaattttga cttgatgtct tctgttggtc
     4441 cattaagtat ttctgtcatc tgtttaccga agtaattcaa atattcttta ccattgaaat
     4501 tttcaccttt accgatatca tcattttctg tcccattctc tttgtcactt actttagcag
     4561 aagaggattc gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt tggttatctg
     4621 tggtagcggt cgtttcttga tctgtgctag taacagatgt ctcaaccgtt gtttcagtat
     4681 ttgtttctgt ttgtgaagta ctcgatttcg tttcatttcc agcatcagta gtttctgtgg
     4741 tttctacctc ccgagattcc gattcggaag aaatgctctc tccatatact gtccattgcg
     4801 ggacgcttgt acttacagca aataagatga ccaacgctcc ctttaatact tgttttgttc
     4861 tcaagctatt cactcctttt tatgtttcta ggagaattat atgtaaacgc aaacattttt
     4921 tatacaaaaa ataaatggtt tatcccataa aaatagaaca ggattatttt ctcattttac
     4981 gtaataaagc agaatggata aaccagtatc cattctgctt tttttaaacg aagaaacaac
     5041 ttattgctcc aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt
     5101 ttcctttgct ttcataaaag gattatcata agttgtcgtt gctcctctac acatcataag
     5161 ctggtatcct aatccatgag caaacttcac agtcgtatct atacaatact ctgcttgtgc
     5221 cccacataat tcgatcgaat gacagtccaa ttcagttaat aaaacactca aattagtctt
     5281 ataaaatgaa ttcgcatggg tttttctcac aaaataactg tgctcaggaa tagataattc
     5341 cgacaatagc tcccatggtt ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa
     5401 aataactggt ttcccaatcg tttcgtatgt tttgattctt ttattcacta atgctgtcac
     5461 tgtttctaat tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta aatcgatcac
     5521 tactattgcg tcggctgtgt ttgacatact tgtgccttct ttcttttttc ttgatttaat
     5581 agtatttctt atctagctag ttccattata cgtgacaaca ttttcttatt ttacacctct
     5641 aaatggcact tttcgactat ttcttcatgt tattatttca taaatactta gctattggag
     5701 gaatgtatga tgattaaatt gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa
     5761 cgttggctta tcgtccatga tataccgttt atcgaacaga acttaattcg tcaaccacta
     5821 accaaaaatg aatttatcca cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc
     5881 tctactcatt ctattagtta taaaaatctt gttcacagaa tagatgaact cagtttgagt
     5941 gaactatata ttgagattcg tacatcccct tctttattga aaaaaccgat tatctacgac
     6001 caatctgcaa aacttttgac tggatttagc gaagataaaa tcaggatgtt cctttctaaa
     6061 actaaaagaa agcaagagct caacgaactc ttgctttctg gtcatcttgt cgattttaaa
     6121 gaaaataatc tttttgcaat gtcttaattt tggcataaac aatctgcttt tcttaattaa
     6181 cgaaatccaa tttttccaat atggatctat ttttctcatt cctgattttt atgatttcag
     6241 ctttcagacg cgcccaatca ttttttgtag gttgtccatt ccagtaaaat atctgtgtat
     6301 tcgtttgatc atatgccttt ttgtcgatag gataatcgct aatgacaata tcaggtaact
     6361 gatcagcgga tgcactatat tttattgggc gttcagtcat agataatatc cttttctttg
     6421 cccataattc ctgtgtttta ccatactttg attgaatata gaatatgaca ggttgttcgt
     6481 gttttataaa aatatccctg actaacatac agtattgaaa aatcaaccgt ctgttctttt
     6541 cataataagt attaagctct ggaattctgc gagctacccc actaaaaaac tgttgcatga
     6601 ttggatacat cactggaaat atatgctgaa aatctctgtc ttctgctgat tttccaaaag
     6661 catccacctt cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt gccaaattga
     6721 catatagata atttttttct gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt
     6781 ctacaataga cgattcaatc cagccccaac tttcttgtac tttatctgga gtatccaaca
     6841 aatcttcttc caaataggtg ttcattacac caaacataaa ataaaggtaa gcttgctctg
     6901 ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat
     6961 gcgtaaactc ttcttttgtt atcaatggat gcacaaattt gttcatctct tctgggacat
     7021 acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt
     7081 tctttctatc tatactacgc gtatatggcg catgtttcgc aaatttttct aatagttgac
     7141 gtttattttt tatacttgat aatatatcag aatgattttc gtcgaatgac tgccaataca
     7201 tcaagtgaaa aaagtatcgg atacggtatt cttctccttc tatcggatta ccttttttca
     7261 agctgcaaga taaatgatag tttttcaaaa tagtattaag ttctcttaat tttctttgaa
     7321 tcgtacgcaa acttatataa ttttttgact aagattttct aaggattgat aagtaccttt
     7381 gtaaagtgaa tccagtaaaa tataaccaat cgt
//
LOCUS       DAYYAJ010000133         7349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000133 DAYYAJ010000000
VERSION     DAYYAJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.133"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..412
                     /locus_tag="ACHATZ_002543"
     CDS             <1..412
                     /locus_tag="ACHATZ_002543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405016.1"
                     /translation="LFGGIIMLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNC
                     NEVIKRKLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRF
                     YNLSGNICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(585..788)
                     /locus_tag="ACHATZ_002544"
                     /pseudo
     CDS             complement(585..788)
                     /locus_tag="ACHATZ_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            754..951
                     /locus_tag="ACHATZ_002545"
     CDS             754..951
                     /locus_tag="ACHATZ_002545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405017.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(994..1569)
                     /locus_tag="ACHATZ_002546"
     CDS             complement(994..1569)
                     /locus_tag="ACHATZ_002546"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8405018.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1724..2250)
                     /locus_tag="ACHATZ_002547"
                     /pseudo
     CDS             complement(1724..2250)
                     /locus_tag="ACHATZ_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2707..4218
                     /gene="gadC"
                     /locus_tag="ACHATZ_002548"
     CDS             2707..4218
                     /gene="gadC"
                     /locus_tag="ACHATZ_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8405019.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4231..5631
                     /locus_tag="ACHATZ_002549"
     CDS             4231..5631
                     /locus_tag="ACHATZ_002549"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8405020.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5796..7231)
                     /locus_tag="ACHATZ_002550"
                     /pseudo
     CDS             complement(5796..7231)
                     /locus_tag="ACHATZ_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2321 a         1222 c         1314 g         2492 t
ORIGIN      
        1 tttatttgga gggattatta tgttagatga tagattgacg tgttctaatt gtggtacatt
       61 attttactat gaggaagtta gaaatattgt aaaacataga aaaaaagaaa tgcctatacg
      121 ttgtccaaat tgcaatgaag ttattaagcg aaagttgtct catggttatt ttgtcagcta
      181 cgaactatat gaagaaagta aaataaatct gaaaaaaaaa ccaaaatact ataaaaattt
      241 gaaaactgga gctattataa ctgaaaaaga atttaaaaat ttagtgactg aagaagaaag
      301 caggttttat aatttgtctg gtaatatttg ccctggattt atggatcaga ttattgaaaa
      361 tgagtcaatt gaatcaattg aaaactatgt tcctgttgac ttaccaaaat agaaaaaata
      421 atcctgtttt tacagtgaat tattttaatt ttgattttca ataaaaatat attacaataa
      481 taatcgtaat agctaatgaa acaatttctc aatcttttta ccactccaaa cagggagcgt
      541 caataatttt gtgtaaataa ctcgtccttc tgcaaaataa tgggttactc agtaaacatt
      601 gaagctaatg tatccgtcac ttgttggaag cctttatggc ttctatttaa gaatttttga
      661 ttgtatgtat caaagatgga tactaggaaa cgctctagtg attcttcatt ttgaaactgc
      721 tcttttctgc ggctgtattt cttgatttgt ttattgaaag attcgattgt agaaccagaa
      781 ggaatggaga acacttttga actttacaac aaaactactg ttgataaatt aattccaatt
      841 ttagttccaa ctgatttaaa tggtcatgat gttttacttc gttttaacgg atttttaggt
      901 gaggattata gatacctaaa aatttctgat cctcaacctt taacagaata ataaaaaacg
      961 tgtaatcaat tatttgatta cacgtttttt atttcaatct tcgttgtagt agtggttgcg
     1021 acataccgaa aggtaatctt cattacctcc tactacaatc tgttcacctg aataaactgg
     1081 aataccatca acaatacgag cattcattac agccttttta ttgcaaaaat ggcaaatagt
     1141 tttaatttcc tcaattttat cagcaaatgt caataatgct tcggatccct tgaagagatt
     1201 attttgaaaa tcattcttca atccatagca aattactggt acttcctcat tcattacgat
     1261 aagtgataga tcaataatat tctctctact taaaaattga gactcatcga ccaaaataca
     1321 atcaactttt ccgtactgat caataatctc cactaattct ccgtacacat ccatttcaga
     1381 agtaattgca atagctggtt tcgaaacacc tatacgactc tcaacttttc caaatcctga
     1441 tcgagtatct attgcgcttg tcattatgat aactctcttc ccttgttctt cataattatg
     1501 agccactttc agtagctcaa tactctttcc actattcatt gttccatatc tgtaaaataa
     1561 ttgtgccatt aaatttattc ccttctttta caattcaatt attccttaat tccatctgct
     1621 tttatgcagc tgctcgttta ttccttgata tgtctataac atctactatc cctaaaacca
     1681 atttataagc cattagagat ggtattcctg aacatctctc atattgtttt ttgagtggta
     1741 tatcatctga aaaactgaag gaatcatgaa aatagcgaca tacttatcat ttagagttct
     1801 aactttaaat gcatttactt cttcagtcag cactttagtc atgttggaca tggtttgggg
     1861 cgtgtagtga tgcccataca ttttctcaat caagtctgcg atttccgaca tggtaatacc
     1921 ttttcggaag aggtgaatga cggtctcctc taatgtgtca ttcgtccgtc tataagcagg
     1981 aacagtctgt tgcttgaact cgccattgcg gtcgcgcgga atctggagat gaagttcccc
     2041 atactcggtc ttgaccgtac ggtcatagga gccgttacga gaattacccg tgttaaaacc
     2101 aatgcgatcg tacttttcgt aatcgaggaa agccgttaac tccgttttga gaagcgtatt
     2161 gacagctttt tccaaatgga cacggaataa tccattcaaa tcgcctttgt tcgctagagt
     2221 ctttaggatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt gaatgtgttg
     2281 tggtaactct attctacaga agggacttcc ttttttctat ttacacaaaa tattttacac
     2341 tctcaacaaa agtatcttcg attaatactt gctcaagtta tgtgcagcta cgaaaaagta
     2401 ggtaccgagg tactttcttt ttcgcagctt tttttggact tatgagaagt acattcgctg
     2461 gaagaatacg atataaaacg agctttttca ctaaagatat taatagagtg aaagaaacaa
     2521 gcttttgttg tcagtttttt cacttttcta aaaatgaaaa taaaaaattc tggaagtgga
     2581 gaagaatttt gcttgctata ctttgtcttg tagaaaagag gtagacaaaa taatgcaagc
     2641 tcaaataagc gattttccat aaccttggta ggaatgacaa attaaatagg aaagggaagt
     2701 aataatatga ataaaaaaaa gttatctcta ttcggttttt ttgccctgac agcttccatg
     2761 gttttgactg tttatgagta tccgaccttt gcaacatcac aattacattt ggttttcttt
     2821 cttcttttgg gaggtatttt atggtttcta ccagttgctt tatgtgctgc agaaatggca
     2881 acagttgaag gatggaaaga tggtgggatt ttcagttggg ttagtcaaac tttaggagaa
     2941 cgttttggtt ttgcagctat ttttttccaa tggtttcaaa ttacagtagg ttttattaca
     3001 atgatctact tcatactagg agcgctgtcg tatgtaatta attttccaga gcttaataca
     3061 gatccattta tcaagtttat tggtgtatta gtcattttct gggtactgac tttttcacag
     3121 ttgggcggga ccaatcgtac cgctaaaatt gctaaagcag gatttgtcat tggaattgtt
     3181 attccttcga tcattctctt tggtttagca gcagcctact ttatcggcgg taatccgatt
     3241 caaatggcta ttaatgcgaa agcttttatt cctgactttt ctcagctttc aactttagta
     3301 gtatttgtat cttttatatt ggcttatatg ggcgtagaag cttcagcatc acatattaat
     3361 gagctggaaa atccccaaag aaactaccca ttggcaatgt ttttactagt gattttggcc
     3421 atcgccttag atgcaatcgg cggtttctca gtagcagcgg ttattccaca acatgacttg
     3481 tctctcagtg ctggtgtaat ccaaacattt gaaacgctta ttttacattt taacagtcat
     3541 ttacattggt tggtaaaagt gattgctctc atgattgcct gtggcgttat gggtgaagtt
     3601 agttcttggg tagttggtcc atcacgaggg atgtttgcag ctgctcagcg aggtctgtta
     3661 ccagttttct tccggaaaac taataaacaa gacgttccag ttccgttgat tatgatacaa
     3721 ggaattatcg ttactatttg ggcagcagtc ttaacttttg gtggaggagg caacaattta
     3781 tcattcttag tagcaatctc cttaactgtt gtcatttatc tcgtaggata tttactattt
     3841 ttcctaggtt actttgtatt agtttttaag aaaaaagact taaaacgaac ttacaatgta
     3901 ccaggaaaaa ctgttggcaa aacgattatc ggtggaattg gttttatttt atcagtgttc
     3961 gcattgctta tctcttttgt accaccagct tcaattgcag ctagtgagca ccgaacatac
     4021 caaatcatac tacttattag ctttgtagtg actgttcttt taccttttat catttatgaa
     4081 gtacatgata aaagtgctca cacaactatt gaggaaccta ctcacgtgaa agctgaagac
     4141 gttaatcccg ctgtttatcc agctgctaga ggagagcacc acgttacaaa aaatgatgaa
     4201 catattttaa aacattagga ggaattcatt atgttatacg gaaaagataa tcaagaagaa
     4261 aaaaactatt tggaaccaat ttttggctct gcaagtgagg atgttgactt gcctaaatat
     4321 aagttaaaca aagaatccat tgaaccacga attgcttatc aattagtaca agacgagatg
     4381 ttggatgaag gaaatgcgcg attaaactta gctacttttt gtcaaacgta tatggaacct
     4441 gaagcagtga aattgatgac ccaaacgtta gaaaaaaatg caattgataa atcagaatac
     4501 ccacgaacaa cggaaattga aaaccgctgt gtaaatatga ttgctgattt atggcatgct
     4561 ccaaataatg aaaaattcat gggaacttca acgatcggct cttcagaagc ctgcatgctg
     4621 ggtggtatgg ccatgaaatt tgcttggcgt aaacgtgctg aaaaattagg tcttgatatt
     4681 caagcaaaaa aacctaacct ggtgatctct tctggttacc aagtttgttg ggaaaaattc
     4741 tgtgtatatt gggatgtgga actgagagaa gtcccaatgg atgaaaaaca tatgtcaatt
     4801 aatctagata ctgtcatgga ttatgtggat gagtacacaa ttggtattgt aggtattatg
     4861 ggtattactt acactggtcg ttatgatgat atcaagggtc tgaatgattt agttgaagct
     4921 cacaataaac aaactgacta taaagtatac attcatgttg acgctgcatc gggtggcttt
     4981 tatgcaccat ttactgaacc tgatctagtt tgggattttc aattgaaaaa tgttatctca
     5041 attaattctt caggtcacaa atatggtttg gtatatccag gtgtgggttg ggtcttatgg
     5101 cgtgaccaac aatacttacc agaagaatta gtatttaaag ttagttactt aggtggagaa
     5161 atgccaacta tggctatcaa cttctctcat agtgcagcac aactaattgg gcaatactac
     5221 aactttgtac gctatggctt tgatggttat cgtgatattc accaaagaac tcatgatgtt
     5281 gctgtttatt tagccaaaga gatcgaaaaa actggtattt ttgaaatcat taatgatgga
     5341 tcagaattgc cagttgtgtg ctataagctg aaagaagatc ccaatcgcga atggacacta
     5401 tatgatttat ctgatcgtct gttaatgaag ggatggcaag tcccagccta cccattacct
     5461 aaagacttgg atcaattaat tattcaacgc ttagttgttc gagcagactt tggaatgaac
     5521 atggctggtg attatgtaca agatatgaac caagcaattg aagagttgaa taaagctcat
     5581 attgtttatc ataaaaaaca ggatgtgaaa acgtacggct ttactcacta aaattcttca
     5641 gagaaatata aaagaagtta attaactaat aaaaggagtt gtgattacca cacagctcct
     5701 taaatctatt tgaataaata tatgatattc atttgttaca ctctcaatct tagctgttgt
     5761 tctattcatc attactctta ttgtaagtag ttcagtcaaa tttgcttgtt caattgatcg
     5821 ataacagtat gaattgtttg aaaagtaatc ggatatataa ttgttttctc actacctaag
     5881 ccttcttggt ataaagaact atcgcaaagt ataatccgct gagtacaaaa gaaagtgtcg
     5941 tctactattt ctctaagttg tttacaaatg atattactaa tagaaaagtg ttctcccaaa
     6001 atgtcttcta actgttgtcg atagtataaa tacctaaagt ctgacggagc tactatgaac
     6061 acttcaattg gagatttccg tttatgctca tttaaaagac ttaaactaat ggttagttta
     6121 cgaatagaat tctcattcca acgcagaaat aaattgtttt gttttcgcca ttgttcaaga
     6181 atttcatgaa cttctaagta caaagcgcgt tgctcttcat ttaacaagta agtcttatca
     6241 ggttgaaata tctgaatatc agcccaaagt gagcggataa acgggagaag tgctttatca
     6301 aaaagtatct gattttcttc agatatattc agagataaga taagtttttg actgagctca
     6361 atcaccaaag ggttatctgc tacatggttt tgataaacaa cgttcaagtc tttttgtaac
     6421 aattcaaagt tatcgcatag ataatttcta gagttgaata gaaagtagat ataaaggcat
     6481 tcatcttcgt catgacataa agagaaagaa agttgctcta atcctttctg tattagctga
     6541 aacaagggag tatgtcgggc aactcttttt tcttcctgac taaaatatat tgggttagtc
     6601 ttagatctct gcaaaccgat agcgatacct aataaaataa attgtttttc aactgtaggg
     6661 taacttcttg aaaaaaatgt ttttctacat aatcaatcaa ttgttgaccg ctttgaataa
     6721 ttgatttatt tagctggatc aactctgtcc gatttgtctc atagattatc gttagtaaca
     6781 aacttcgata atccttttca ggaatttcaa aatggccatc agcatttaag tactgcattt
     6841 gtttaaaaaa attaatcaac tcatttcgta agttgtaaac tttgcttaac gaaatgaagg
     6901 tagtatcagc tatattactc caattgtgtt cccctgataa ataatgtcct agtacattga
     6961 aaaagtcaga gcgctgatag atgagttgag tgagtgccaa cctcgaaaac gaagagtcga
     7021 atgcgactgt aaagctactt ctagatttct ccactgaaat aatttgctga tggagtatgt
     7081 cggtaatgca ttccaagtcg ttagtgacag tgagtgggca aacgttcaat tgatctgcta
     7141 tttcgcgaat ctgaatggtc cctttttcgt gtaattgctc acaaatgtaa acttgacgaa
     7201 aaatactttt ctccagatag gtttccaaca tagtactcct cctagtcatt ttatacataa
     7261 ctattataac tctaccaatc ggaatagtcc tgtaatatat tgaatcaaaa atataaatta
     7321 taaaataatt agatggggta tttggttta
//
LOCUS       DAYYAJ010000134         7343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000134 DAYYAJ010000000
VERSION     DAYYAJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.134"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..624
                     /locus_tag="ACHATZ_002551"
     CDS             217..624
                     /locus_tag="ACHATZ_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405021.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            638..1039
                     /locus_tag="ACHATZ_002552"
     CDS             638..1039
                     /locus_tag="ACHATZ_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405022.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1041..1412
                     /locus_tag="ACHATZ_002553"
     CDS             1041..1412
                     /locus_tag="ACHATZ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405023.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1448..1747)
                     /locus_tag="ACHATZ_002554"
     CDS             complement(1448..1747)
                     /locus_tag="ACHATZ_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8405024.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1753..1983)
                     /locus_tag="ACHATZ_002555"
     CDS             complement(1753..1983)
                     /locus_tag="ACHATZ_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405025.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(2419..2985)
                     /locus_tag="ACHATZ_002556"
     CDS             complement(2419..2985)
                     /locus_tag="ACHATZ_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8405026.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(2985..3941)
                     /locus_tag="ACHATZ_002557"
     CDS             complement(2985..3941)
                     /locus_tag="ACHATZ_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8405027.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(3941..4597)
                     /locus_tag="ACHATZ_002558"
     CDS             complement(3941..4597)
                     /locus_tag="ACHATZ_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8405028.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            4967..6985
                     /locus_tag="ACHATZ_002559"
     CDS             4967..6985
                     /locus_tag="ACHATZ_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8405029.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    7055..7261
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2491 a         1306 c         1355 g         2191 t
ORIGIN      
        1 acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt tactactgat
       61 taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt agctaatatt
      121 tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa gtgaaacata
      181 aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt acaattaata
      241 atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa agtaattaat
      301 ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc tgtaacagct
      361 gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga agttttaaac
      421 aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc tcctcaagtt
      481 aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc aggagagcat
      541 tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga tatcaaggtc
      601 aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag taccatcaaa
      661 tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg catcggtaat
      721 tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa atgataatca
      781 acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg gcaaaatttt
      841 tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat atgatgaaga
      901 tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct cagattacga
      961 tgttttggat actctcgata atgtattatc tgactataaa tatatgggga aatctgtagt
     1021 agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt tccaaacaaa
     1081 ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct gtttcaagag
     1141 catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat gatattattt
     1201 ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt tcaacagtta
     1261 aggaattgaa taccttagga atacataata gtgggtatag aatgttagag aaaagaaaat
     1321 gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg atgaatcgag
     1381 aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt gtttgttatt
     1441 tttttattta atctttgtat ctttctggat caacgaaagt atactttata tagtcataac
     1501 gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa aagtatacac
     1561 ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta gaatagtaga
     1621 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc catactttat
     1681 aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt gtctcattaa
     1741 aaagcataac cattactccg atctgttttt aaaattagtt tctacctcta atatatatcg
     1801 agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag gttgtttatt
     1861 ttcggaaaaa gctaatatag ctaagattga aacatccatc tggaatttat cttttttact
     1921 gctttgatca taaaagtctg catattcatc actgatattt tttctaataa attcttccat
     1981 cataaaaatc acctcaaaac gattatacga acttacgttc ttgttgtaaa gcgatatttg
     2041 aggggcaaaa aaggggcata atttgactga tattaagtta gctattgata tatgaatgac
     2101 atacaaaaat ataaaatctt ttaaaaatgc ttatatatca atgtttttgc cattctttta
     2161 tatatctgtt atatgtcaaa aaaatgtgtt caatttcaat ttgtatttga ataaagaggt
     2221 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg
     2281 tttttgattt ggttggactt attaccgcag atataagctt ttgagcaccg tgaataaaga
     2341 ggttatgaaa tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa catatcattt
     2401 atgttttgcg ttttttattt atcttttttt ctcaatacaa tataaaatag gcggctggtt
     2461 cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca gtttggaaca
     2521 aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt agacgacaag
     2581 gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg tgcgtgtagt
     2641 ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat tgaaaatccc
     2701 tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc cttgtagaaa
     2761 caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag catcgattgc
     2821 ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt ctgctagaaa
     2881 aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg gttgcaagat
     2941 ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac gctcaccttt
     3001 ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc atcaattgca
     3061 ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc ccacggcgca
     3121 tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg gtcgcaaatg
     3181 acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata gtcccgcaac
     3241 attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat atctgaatct
     3301 aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg taatccgttg
     3361 atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc atctaaataa
     3421 gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt tgtttgcaat
     3481 ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc aactacttca
     3541 tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg taaattgact
     3601 acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt aaaattttgg
     3661 aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc gatttctttc
     3721 tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga accagataca
     3781 ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca gtcaaatcct
     3841 ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc gtaattccaa
     3901 gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc ttctttctgt
     3961 aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg cacagccaag
     4021 aaagactgag taagtaaccg ccgatgatat cagtaggaaa atgaacgccc agataaatgc
     4081 ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat gtgcgtaagg
     4141 ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga atcatactgc
     4201 ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc gccaatgaag
     4261 gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca attacaataa
     4321 atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc cataaagccg
     4381 cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata aagagttggt
     4441 agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga tcgaaaccag
     4501 aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg acaagaaaga
     4561 agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt tgaagcaatg
     4621 acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa ccaatgtatg
     4681 attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg aaagacattt
     4741 tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa atccttttaa
     4801 acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat tacggtaaat
     4861 tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa tattaagaga
     4921 agttataatt attaataata gataaattga agttggagga tagaagatgg aaaacattgc
     4981 aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc
     5041 agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt
     5101 tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt
     5161 tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc
     5221 agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc
     5281 accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga
     5341 tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc
     5401 ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa
     5461 cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc
     5521 ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat
     5581 cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc
     5641 gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg
     5701 taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt
     5761 ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat
     5821 ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg
     5881 ctcttcgacg aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa
     5941 cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat
     6001 ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa
     6061 tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc
     6121 aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc
     6181 taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc
     6241 aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt
     6301 tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg
     6361 taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc
     6421 gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa
     6481 cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc
     6541 aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac
     6601 ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg
     6661 taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca
     6721 tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg
     6781 acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg
     6841 ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg
     6901 aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg
     6961 tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac
     7021 aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa
     7081 aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact
     7141 cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca
     7201 acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata
     7261 aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag
     7321 caaaaggaaa taaaaataaa cag
//
LOCUS       DAYYAJ010000135         7289 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000135 DAYYAJ010000000
VERSION     DAYYAJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.135"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..622
                     /locus_tag="ACHATZ_002560"
     CDS             <1..622
                     /locus_tag="ACHATZ_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405030.1"
                     /translation="ANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVN
                     TVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQW
                     IKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRI
                     DNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            805..3480
                     /locus_tag="ACHATZ_002561"
     CDS             805..3480
                     /locus_tag="ACHATZ_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8405031.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3662..3901
                     /locus_tag="ACHATZ_002562"
     CDS             3662..3901
                     /locus_tag="ACHATZ_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405032.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3917..5290
                     /locus_tag="ACHATZ_002563"
     CDS             3917..5290
                     /locus_tag="ACHATZ_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8405033.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5294..6928
                     /locus_tag="ACHATZ_002564"
     CDS             5294..6928
                     /locus_tag="ACHATZ_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8405034.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7091..>7289
                     /locus_tag="ACHATZ_002565"
     CDS             7091..>7289
                     /locus_tag="ACHATZ_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405035.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2599 a         1136 c         1449 g         2105 t
ORIGIN      
        1 tgcaaactca ttctggtttt taaaaaatat taataaaaca gtggaaaaga atggagttta
       61 taaaggtgat tttcattttc aaaatgctga gactattgaa cgagaacatc attatttcga
      121 tttattagtg aatacagtgg tagaattagc gaaatgggca aagtcaaacg aaacttatag
      181 tcaagtactt aaaagaatta tgagtcaaga aacttcattt atctatgagt taagcaagtc
      241 aaatttgaaa tatacttata atgaagatga acttgaaatt ttagataatc cagaaaattc
      301 acaatggatt aaagagttga agagagaaga ctttattgag acactaagaa ttctaaaatc
      361 gttatttgat ttgaaattag ccaaagctac catcatggga attgaaagag attcatatta
      421 ttgggaggaa attggactat atgctcccca gattgaaggt gaagataaag agaaaaggat
      481 agacaacatt attaactata ttaatagttt aagtgaacct ttaaaatgta gaggacttga
      541 ttttatagga acattaggat ggacagaaac atactatgag aaaatatata aagaaataat
      601 tgaacaatat tttcctaaat aatttaataa ggattgattt atcgtaaaag aaaaaagttg
      661 tgatttccaa aatcacaact tttttctttt gtcaagattt tgagtgggat atatttttgt
      721 agaactgaag atcatgaaat tatgagaggt tggaggtaat gtagtcatac aaataaaaat
      781 tttttagaaa gtgagtggta attaatgaaa atgaatcaca cttcaattca gaagcattta
      841 caactgttaa atacactggg gatttcttat gttaatttga gaactttttc tacattaaac
      901 aagaaaaccg tttctcatat tttttctact gcagattctc aagcaattat taactattta
      961 gaaaaacaga aattgtctga aggtacagta aacataactt ataatcctat taaacaagag
     1021 gtgattacac aaaaaaagca ttctgtgaga gacaatgata ttgaaaaaat tcggtttgta
     1081 tttattgata ttgaccctaa aaggaaagag ggaagtgcaa cagatagcga aaagaaaaag
     1141 gcagaaaatg ttatggaaca agtagaacgt tatttgaaag aaaagagaat agaatcattt
     1201 gtaaaagttg atagtgggaa tggctaccat atttttcttc ccataaatga acaaccaaat
     1261 aatcacgaga cgattcttac tattcagaat tttcttcaat tacttcaccg aagatttgga
     1321 gtagaagatg aagttgatat tgatacttct gtttataatc cttcaagatt atgcaaatta
     1381 gttggtacac cagcggtcaa aggaaagtcc acaaaagagc gtccgcatcg tttatctagt
     1441 ttcctttcta ttcccgataa catagataga agtaattgtt ttaaaattct tgaacagata
     1501 gttgaagaaa attccatgat gtcaacctct agagtaaaaa gccatccatc ttttagagaa
     1561 gtgttacctt ttattcaagc agactataaa caatggttag ctcactatcc tgaattaact
     1621 tataccataa aagagggcga tcataaagga gttaccttac ttatatttga cgaatgtcca
     1681 ttaagaaaac acacgaataa ttctaatggt tcttgtttgt ctgttacggc tgataataag
     1741 attcgatttc attgtctgca tgcttcggat aaaaataaag atattcaaga ctttgtaaaa
     1801 aaatatccgt tgccaaaacc tgtaaatcct tctgtagaat cattagaatc caatcaagaa
     1861 tattattttg accagtttaa actgaatcaa gaaggagtat ttttggtgaa agaaggtcaa
     1921 gaagaaaaag ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat cgaacaaaaa
     1981 gtcattacac ttgttcttgc ctattatgtc gatcattgtt gggatgaaat agaaatagaa
     2041 ggtggatgtt tacaaactaa taattttaaa caattagcaa aacatggctt aatcattaaa
     2101 gctagaagag aagctgaagt cgttgacttt ttacagattc aacgggagaa tgctccaatc
     2161 ttttttgaac atgaatctct tggctggaaa aaagaaaatg aagcaatgat ttttcagtta
     2221 caagattctt ttggacgtga aattccttct gtattaacta aaaactcatg ctaccaatta
     2281 acaactaatg gtacagaagc ggaatttgac gcattggttc ataagtatgt tttaggaacg
     2341 aacttagaac ttgcttggtc gattggtatt gcttctattg tgttaggcta tttaaacgtt
     2401 acgaaggcta atactttttt gagtttacta attaacctac agggcaagag cacaacgggt
     2461 aaaaccacta cgttacactt tattgcctct ttatttggaa atccaactac ggttcttaga
     2521 aatatgaatg ctaccaataa agccattata aagctagcat caaacaatca gggaggtgtt
     2581 ccactaatac tagatgagtt aggagcagca actcaaaata actttagctc gttgatctat
     2641 caattagctt caggagaaga aaggctaaga ttagattcta attgtcagct tatgccacaa
     2701 gagacttttt ctgttttgac ccttacctct tctgaagagc gtttggaaaa ttatttcaat
     2761 gagaaattaa ctggtttgaa agttcgatat atcgaatttt ctgatatcaa gtttacaaag
     2821 gatgcgaatc acgcagagaa agttaaaagt ctttcttcta atacttttgg ttggggtata
     2881 aagttatttc ttttgaaact ctttgaacaa gggattacaa aaattgaagc aacttttgtc
     2941 gaagctcaaa aaaaactatg ggctgtttta cctgaacatt ctttgaagca acgcattggt
     3001 aataatctag ctttgattta tagtggagcc ctcctaattc aagatatact gggctatcct
     3061 ttacgattag agcatataca gacggtgctt attaattcgt acgaacagtt aattgtagat
     3121 actagtttag gaagtgagca aacggtattt gaccgagtga aaacattgtt ggtgatgaat
     3181 agccataaat ttttaacaaa cagtgacaac aaacatacgc ccagtacgat ttggggaaaa
     3241 gtatcaattc aaaaaaatgg ggatattaag gtaaatattt ttcctatccc gtttgaggac
     3301 ttgttaaggc gagaatttca agtaaaggat ctaacctatc tttttaggca attgcttgat
     3361 tcgggtaata tgtgtgcaga aaaagggaga agaacgaaac gtattcacat gaatcgtcaa
     3421 gttcttccaa catacgagat tctattacct tcttcactca aatcgtattt tcgattgtaa
     3481 tttagaataa accaaagtat gaaagtgagg caccatgtta tggaaatatt tcttattgat
     3541 ttagcaaaac aactgattac ttatctgtat gatagcgagc aaacccaaac ggaagtaaat
     3601 gaagcttgat ctatgcttca tttacttcat tcaatcaaac atgaaagaaa ggaaaattct
     3661 aatgactaaa aataaaatga cactaaaagc agaggttctt ctgtatattc aagaacattt
     3721 ttcaaatcaa gcctttttta caaaaccaat ttacttggat ttcgaaataa gaggggtatc
     3781 tgctggatct atcgggggta ctcttcaagc gttgaagaac gaaggatatt tagaaaatca
     3841 ctttgtgcaa cgttcgttca atagaagagt cgtgaaaaaa tggtatttag ttcattcata
     3901 gagaggggaa tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt atgaagaagc
     3961 gaaaattagt aatgacgctc aatatgatgc agtgaaagag tatgttttag agaaatatga
     4021 acaaattccc caacgatttt cagacaaagg agaatcaggg gaaaaagcgg agcgtccagc
     4081 ttttcgacag cttagagctt gggtttgtgc gtcagaagaa gagggggtga tctatgtttg
     4141 gcgttatgat cgattggcac gtcaagccaa aatagcctta gaatttattg agttgtgcca
     4201 acaacatcag gttcaagtgt attcgatttc tgaacctctt cctgcttctt tctcacacgc
     4261 gtcaacgaat aaactatttg tgcaattatt gttcactttt gcagaacaac aacgaaacgt
     4321 cattattgag aatgttcgtg ccggactttc ctataaaaag caacaacggc aatatttgtc
     4381 ttctgcggta ccatatgggt atcgtttagt gaatgggaaa atccaacaag aagtacacga
     4441 agcaaggatt gttcgctata tttttcaact ttatttgacc aaaaaatatg gctacaaaaa
     4501 gctttgtcaa cgattgacgc aacaaaagct tttcttccgc gaacgtcctt ttcagcccta
     4561 tcatatttat agtatcctaa aaaatccatt gtactatggc gaaatcaaag gaggatcttt
     4621 ggggaagtat ctaggaacct ttgaaccgat tctctcgaaa gcaacctttt tacaagccca
     4681 agagattcgc caatcaaggt gtacagctaa aaaagatacg tatctgtact tattacgtca
     4741 aaaaattcgg tgtcccttct gtggtcgtca tctgagttcg aagtatcaat ggaatacaaa
     4801 gaagacgaag acgcttcatt attatcattg taccgaccga agctgccaag gaatttttct
     4861 tcgggcagaa agggttgagt cagaagtatt ggaaaagata caggcttttt ttcaatacga
     4921 aggcgtctat caagccttga ttgcccaatt aacgcaacaa ttagccgaaa agcgaaaaga
     4981 tgaaaaacgc aaagtaatcc aacaaaagaa acaagaagca caatggatta aggcatttga
     5041 agagggcaat tgttcactgg aagaactcaa gcaacaactg caagctcttc aaaagccgat
     5101 gtctacttct tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag aatggcttca
     5161 actaaaagac ctctctagtc gacaattatt atgtgactat gtttgtcgga ttgaggtaac
     5221 aaaggataaa acagtttgtg ggatttactt ccgacaactg acacaaaata ttttagaaag
     5281 gaacgaatga tctatggaaa tgatacaaac caaacgagtc gcattgtata ttcgcgtgag
     5341 tacacaagaa caagctttag aaggacatag tattcaagca caagaacaaa atggacgaca
     5401 gtttgccgaa cggatgggct atgaagtggt agaagtttat gccgatgaag gaatcagtgg
     5461 gaaatccaca aaaaatcggt tcgcctatca acgaatgatg aacgatgctc ggaaccatcg
     5521 ttttgatcta ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt tagatgtctt
     5581 acaaacagtc gaggaattat tgcgatatga cgtaggatta cattcgatct cggaacaatt
     5641 tgacgtgacc acttcgacag gtaaattgat gttacaactc cttgggagtt tcggtgaatt
     5701 tgaacgcaac caaattgcgg agaatgttca aatgtcaatg caaagcttag tgaaagataa
     5761 aaagcgttat gcaggaggcc gacgtttagg ctatgtcagt ggtattgaca cgaatggtca
     5821 aaaacaattg gtcattgaac cagaagaggc gaaaattgtt cgtctgatct atgcaaaata
     5881 tttaggcgga gcagggtatc gagcgattgc caattttttg aatcgtcaag gatatcaaac
     5941 agtcaaaaag aatgcttttt ctaccacggc agtcaaagat atcttacgca ataaaattta
     6001 tgcagggtat ttagaatatg cgcgctacat caattgggaa accaaacgac ggaaggggaa
     6061 gaatccgaga ccgattttag tgaaaggaac ccatgaaccg attatttcgg aagaagatta
     6121 tcaaaaagta caagaacgcc ttgctttaga aggaaaacaa cccaaatgga accatacagg
     6181 tgaaaatgta ttgacaggct tgttacgttg tccagaatgt ggcgcaccga tggcagcgag
     6241 taatgtcaca aataccctaa aggatggcac aaagaaacgg attcgatatt attcttgttc
     6301 agtctttcgg aataaaggag caagtgtgtg tcatgccaat tcgattcgtg ccgaacaagc
     6361 ggaaacattt gtcgccgacc gattaaagga gatcgttcag ctaccagaag ttcttccccg
     6421 attggtggca gcgttaaatg aagaaatagt gcgacaaagc caaccattgg aacaagaact
     6481 cgtggtgtta ctggaacgaa aggaagaatt gaagactaaa attgaaaaat gggaagctgc
     6541 tttggaagat agtcctgaat tgtttccgat gttaaaagac cgcttagacg aattgactga
     6601 aaaacgacga cagttgcaca ttcgagaaaa tgaaatctta gggatctttc aacaaggaga
     6661 gccgattcaa gtcaaagatg tccaacggat attgactagt ttggatcgtt ttcttgccca
     6721 gagtgagaag aagcaaatca aagccttgta tcgtaccttt atagagaaaa taacctttga
     6781 tcctaaccat aaggagatgt tggaaattac gatgaaattc acgcatgccg tggtacaaca
     6841 attgaatgag caatatcaaa tagccgtatc caaaacaaag gatacggcta tttttgtgtt
     6901 aaaaacaccc tttacgttaa cgatctagtc taggttatat caaacaacgt tagaggggcg
     6961 ctaacgcttc tctagcgatt tgaaatcgtt agagaagtag ttagggaggt ccttatcata
     7021 aatagggata tttattaatg aatatttgaa atataattgt atataagaat taaaaagagt
     7081 ggggatagat atgaaaaaga tgggtaagtg tattttatgt gaaaaataca ctgaacttac
     7141 taaagaacat gtaccaccta aaaaatcagg gaatactgga ggtaaaaaga ccagaactgg
     7201 taatttagat gatttcctta agagcgattt tacaaaaggt gattttccga aggggattaa
     7261 aaggaaacct caaggaaatg tttattata
//
LOCUS       DAYYAJ010000136         7129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000136 DAYYAJ010000000
VERSION     DAYYAJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.136"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACHATZ_002566"
     CDS             59..415
                     /locus_tag="ACHATZ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8405036.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACHATZ_002567"
     CDS             405..671
                     /locus_tag="ACHATZ_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8405037.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACHATZ_002568"
     CDS             830..1831
                     /locus_tag="ACHATZ_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8405038.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYIFISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACHATZ_002569"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACHATZ_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACHATZ_002570"
     CDS             3055..3300
                     /locus_tag="ACHATZ_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405039.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACHATZ_002571"
     CDS             3398..3619
                     /locus_tag="ACHATZ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405040.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACHATZ_002572"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACHATZ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACHATZ_002573"
     CDS             4761..5300
                     /locus_tag="ACHATZ_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405041.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6320..>7129
                     /locus_tag="ACHATZ_002574"
     CDS             6320..>7129
                     /locus_tag="ACHATZ_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8405042.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEIC"
BASE COUNT         2609 a          977 c         1258 g         2285 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt tttatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
     6001 tatattttag gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa
     6061 tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt
     6121 aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag
     6181 tgatataacg aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga
     6241 tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat
     6301 tttataggag gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa
     6361 taaaggtgat ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt
     6421 gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc
     6481 aggtaattcc cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa
     6541 tttggtaatt cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa
     6601 aagaaccaat gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat
     6661 gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat
     6721 ttcaaatatg actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga
     6781 atccagatac tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt
     6841 ttcaaaagaa gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct
     6901 tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct
     6961 aaaagaccgt ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa
     7021 cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc
     7081 tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgt
//
LOCUS       DAYYAJ010000137         7086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000137 DAYYAJ010000000
VERSION     DAYYAJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.137"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..366)
                     /locus_tag="ACHATZ_002575"
     CDS             complement(142..366)
                     /locus_tag="ACHATZ_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405043.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(386..808)
                     /locus_tag="ACHATZ_002576"
     CDS             complement(386..808)
                     /locus_tag="ACHATZ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8405044.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(950..1660)
                     /locus_tag="ACHATZ_002577"
     CDS             complement(950..1660)
                     /locus_tag="ACHATZ_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8405045.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1678..2175)
                     /locus_tag="ACHATZ_002578"
     CDS             complement(1678..2175)
                     /locus_tag="ACHATZ_002578"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8405046.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2212..2664)
                     /locus_tag="ACHATZ_002579"
     CDS             complement(2212..2664)
                     /locus_tag="ACHATZ_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8405047.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2799..3404)
                     /locus_tag="ACHATZ_002580"
     CDS             complement(2799..3404)
                     /locus_tag="ACHATZ_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8405048.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3854..4474)
                     /locus_tag="ACHATZ_002581"
     CDS             complement(3854..4474)
                     /locus_tag="ACHATZ_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8405049.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4476..5345)
                     /locus_tag="ACHATZ_002582"
     CDS             complement(4476..5345)
                     /locus_tag="ACHATZ_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8405050.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5368..5775)
                     /locus_tag="ACHATZ_002583"
     CDS             complement(5368..5775)
                     /locus_tag="ACHATZ_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8405051.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5794..7083)
                     /locus_tag="ACHATZ_002584"
     CDS             complement(5794..7083)
                     /locus_tag="ACHATZ_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8405052.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         1927 a         1499 c         1180 g         2480 t
ORIGIN      
        1 aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc
       61 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
      121 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc
      181 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
      241 atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
      301 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
      361 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
      421 aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc
      481 ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag
      541 gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta
      601 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
      661 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
      721 tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt
      781 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
      841 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
      901 ttacaaaaaa aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg
      961 cttctgattc atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga
     1021 ttttttcctt caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg
     1081 attcaatgac cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac
     1141 gccagttgag acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa
     1201 gtgccggagc atcggaatct gcacctaaag gcaggaatga ttcgccacct tgggcttttc
     1261 ctaaatgcag atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag
     1321 acaaggaaat actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt
     1381 gatttgcacg tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg
     1441 tggtaaatgc ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa
     1501 ggtaatttct tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt
     1561 cttttgttac gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa
     1621 atacttccca gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta
     1681 gaataattgc ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg
     1741 ttcgctgtat ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc
     1801 ccaaacaccg agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc
     1861 tagtaaaaat gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat
     1921 ttctaaaaaa tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta
     1981 acccgcaggc tcttgatcga tactcactag gaaaaaatcg gtttctttat cttttagctc
     2041 ttctgtaagt ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtga
     2101 attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta gtaagtctac
     2161 atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct ttcaatagat
     2221 ccttttattg ccttttttga caaaattcca atcttcgcta acattcttgc atactttatt
     2281 taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt
     2341 ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt tagtcggata
     2401 gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc cattggattc
     2461 taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt ctgctagttt
     2521 ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact gtcctcttgt
     2581 taaatcgact tctttgaatt cgatattgct aatagaatct agcgctcggg caatcattcc
     2641 gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta agactagttc
     2701 acctcttttt gttgcaaatg caactatttt caataaaaaa atcaagttct tttttaataa
     2761 ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata gatctggttt
     2821 ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag ctttggcaac
     2881 tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa cttggcctct
     2941 tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg tggaatatct
     3001 ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt taatcctgtt
     3061 tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc agcaaatcca
     3121 ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt gccatctttt
     3181 ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat cgttgtgagc
     3241 gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa tgaaatcgtc
     3301 cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag accagcttga
     3361 gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt cccttctttt
     3421 ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac ggcaaataaa
     3481 tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa aagacattcg
     3541 acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt aataagcccc
     3601 accaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat tgaggcagga
     3661 cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc ggtcacaagt
     3721 cacaatattc atcaaacatg agaagctgtt tttttgaaat tgctccttgt gactcggagc
     3781 tgaacgacag gctcacgacc tctcggtaaa cagcgttcaa aagccaattt ttcgatatta
     3841 agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc aatagtggag
     3901 tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct aactggaatt
     3961 tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata tgattggtcc
     4021 ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata tagctcaaat
     4081 gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca aagtctgctg
     4141 ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg agtcgtgctt
     4201 tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct tccatataca
     4261 aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt
     4321 ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg agcgtgatgg
     4381 catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt cctgtaccag
     4441 gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa gttaattgga
     4501 ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac tgcttcattt
     4561 gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa ggtttcttta
     4621 gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg gatcccgtta
     4681 ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat aactaggggg
     4741 acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt ttttgatttg
     4801 aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt agcttctaca
     4861 aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac atggattcct
     4921 tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg cgcatgggca
     4981 tattctacta ctgcttttgt cttcaggata ttttctttga aaggcagatg ggacccatca
     5041 tacatgacag aagtatatcc gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg
     5101 tggtctaagt gcaagactgc atcaaccatt tcttcttctg caatagctgt cattacattg
     5161 actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat aatgatcggc
     5221 gaacccattt cttttgctgc gcctagcata gcaggaagca tttctaggtt atgcgtatta
     5281 aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt caatgttgta
     5341 tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta cttgttttgc
     5401 aatttgtcgc atctcatcca ttttgcccat gtagtaatcg atcccttgat catcaccagc
     5461 acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat acccatctcc
     5521 gatcgtctgt ttattttcca aactcttttc taaatattgg atggcttgat cgttcacata
     5581 caattcagaa aatttaagtc caacttgttc ataaagtttc gctaatttat gcttatcttg
     5641 ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt cggtcgcttc
     5701 tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt cttttttctt
     5761 aaaaaatcca aacatcattt tccctacttt caattaaaat gctttcaaaa ctgggcttaa
     5821 gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg tagcattgtt
     5881 gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa taaagaaacc
     5941 atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc caaatatccc
     6001 tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg ctaagccgaa
     6061 catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga aacctaaaat
     6121 gacagcattt gggctataag gaaagaaaac tggacaatct aaggcaggga tcgcatcagg
     6181 aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac
     6241 accagctagc aatacataca cacccactac gaattggatg gcttgtaaaa aggcaaacat
     6301 gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa ataaaacagt
     6361 gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt gcagaaattc
     6421 gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt tctcaccaaa
     6481 aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac ctaaagcaat
     6541 atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca tagcaatggc
     6601 gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca gaccggcaaa
     6661 atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat
     6721 gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg ctaagaacgt
     6781 cagtgcaata tctctaccta aacccaaatc attcattgct tgaccattga tagattcaat
     6841 agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga aataagttaa
     6901 acttccaacg atgatgctcg aaccggcact caacacttga aatcctagga gtgtctttaa
     6961 cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca acatagcaat
     7021 caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat tgataacgcc
     7081 catgtt
//
LOCUS       DAYYAJ010000138         6874 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000138 DAYYAJ010000000
VERSION     DAYYAJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.138"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..452
                     /locus_tag="ACHATZ_002585"
     CDS             225..452
                     /locus_tag="ACHATZ_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8405053.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            468..1634
                     /gene="hemW"
                     /locus_tag="ACHATZ_002586"
     CDS             468..1634
                     /gene="hemW"
                     /locus_tag="ACHATZ_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8405054.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1869..2912
                     /gene="hrcA"
                     /locus_tag="ACHATZ_002587"
     CDS             1869..2912
                     /gene="hrcA"
                     /locus_tag="ACHATZ_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8405055.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3006..3569
                     /gene="grpE"
                     /locus_tag="ACHATZ_002588"
     CDS             3006..3569
                     /gene="grpE"
                     /locus_tag="ACHATZ_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8405056.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3620..5449
                     /gene="dnaK"
                     /locus_tag="ACHATZ_002589"
     CDS             3620..5449
                     /gene="dnaK"
                     /locus_tag="ACHATZ_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8405057.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5600..6766
                     /gene="dnaJ"
                     /locus_tag="ACHATZ_002590"
     CDS             5600..6766
                     /gene="dnaJ"
                     /locus_tag="ACHATZ_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8405058.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2354 a         1178 c         1545 g         1797 t
ORIGIN      
        1 ataattggaa tctagttaaa actcttgaag tgagagattt gactagattt ttttatgaat
       61 aaaataagga caaaattgtt taggaagaat catgagtgta aaaaaatcat ccaagaagtc
      121 gattcttgga ttttttagtg gatttgttcg ccaaatacgt ttagtatgcg tatatgagag
      181 gttgtaagca aagcgttgtg gtctgagcac ttaaggagga atctgtgaaa atagcttttc
      241 atatttttgc agattttgac ttaatgccga aggtcagctt tgcgattacc gtttatcgag
      301 aggctgtaaa agcaccgtgt agctccgatg acaataagca agcattttta gagctaggtt
      361 tccttgattc taaatatgcg gcttatttca atgagttggt gcttgaacgc gattttaaga
      421 aggtcgaaaa caggcacgac ttttcgacgt aaggagaaag aatttcaatg atagaaacaa
      481 taaatcccgc agaaaacaag gtttctgcgt acattcatat cccattttgc gagcatatct
      541 gctattactg tgactttaat aaagtttttt tagaagggca gccagtagat gagtatgtgg
      601 aaatgctgct gaatgaaatg agattaatgc tggaaagtca tcctacagcg aatttggaaa
      661 cactttatgt aggaggcgga acaccaactt ctttatctgc taaacaactt gatcgactat
      721 tatcaggtgc tagagagatc ctgccttttc aaaatggtaa tgagtttact gtagaagcga
      781 atcctggtga tttgacccgt gaaaaacttc aagtgatgaa aaactatggc gtcaatcgtt
      841 tatccatggg agtgcagaca tttgacaatc gcttattgaa aaaaattgga cgtaaacata
      901 ctgcagaaga tgtgtatcaa acaatgcaat ttttagaagc agaaaatttt accaatgtaa
      961 gcatcgattt gatttatgca ttaccgggac aaacactaga aggatatcgg gatactttgg
     1021 aaaaagcttt ggctttggat ctgcctcact attctttgta ctcattgatc ctcgaaaaca
     1081 aaacaatgtt catgaactgg gtacgacaag gaagactcga actgcctgat caggaaacgg
     1141 aaaccagaat gtttgaagag acgattgaag caatggaaaa gcatggacgt caccaatatg
     1201 aaataagtaa ctttgggctc tccggacatg aatctcaaca taacctcatg tactggaaca
     1261 atgaccacta ttttgggttt ggagctggtg ctagcggtta tcttggagat attcgttacc
     1321 gcaacaaagg accaatccaa cactatttac gcccattacg agcaaatgaa cgcccggtat
     1381 tggaagaaga aaaattgaca cgaaaaaatc aaatagaaga agaaatgttc ttaggactaa
     1441 gaaaaaaaac agggattact aaaaaacatt tctatgatcg ctatcaaaaa acaattgaat
     1501 cggtgtacgg tgatgtgcta agggaattag agcaagaagg gttacttgtt aatgaagaag
     1561 accgtgttta tttgacacca agagggactt ttttggggaa caatgtgttt gagcgctttt
     1621 tatttgaaaa atagtttgaa gaatatttgt tttttatcga aacttgaaaa gaaaaagttt
     1681 agaaagtttg acaatgaggt attgaagtat tgatataact ggatttacat caattattta
     1741 gaattagcac tcttgtgttt agagtgctaa aaaatggagt ctttctcttg acaggagaag
     1801 ggcttcttgc tatatttata agtgaattag cacttagaat atatgagtgc taatgagagg
     1861 tgaggaagat gttaacaaag cgacaaaacg acattttacg tctgatcatc caacactata
     1921 cgagtagtgg cgtgcccgtt ggttccaaaa cattaatgtc tgagggagtc aaggccagct
     1981 cagcaacgat tcgcaatgat atgaaagcat tagaagatga aggattgctg ttgaagactc
     2041 actcttcttc tgggcggatt ccatctgctt taggctatcg ttattacgtg gatcatttgt
     2101 taaaacctgc tcgcgtagca gaagatgatt tgctgctgat tcgtcagtct ctaggcagag
     2161 aatttcatga aatcaatgag attattaagc aatctgctga gatattatct gagctgacaa
     2221 gctatacgac tttttcattg ggacctgaaa taaaagaccg ccgactgact ggctttcgta
     2281 tcgtaccctt gaataatcga cagattattg ctatcgtcgt aacagatatg gggaatgtag
     2341 aaagccaagt gttcactctt cccgaatata tgggaagtca ggaactagaa aaaatggtac
     2401 agattgtaaa cgaccgtcta gtcggagatc ctctgatgac ggtgtaccat aaacttcgta
     2461 cggagattcc gatgattctt cataaatatt tccagacgac agaagggatg tctcagttat
     2521 ttgacaccgt tcttggtcat gtttttgaag aaaaggttta tgtcagtgga caaatgaatc
     2581 tcttggatta tgagccccgt caagatcttg atcagttcaa atcgatgttt tcttttatga
     2641 aagactcaga cggacttaca caaatgatca ctccgatgga tagcaatatt catatcaaga
     2701 ttggtgctga attaggcaat gatttattgc agaatatgag tatgattcaa gcaagttacg
     2761 agatagatgg tcatggaaga ggtacaatag ccttgttagg gcctactagt atgccttatt
     2821 ctcggatgtt cggtctagtc gatgtttatc gaagagaact agcagctaaa cttgctgatt
     2881 actaccgctc gcttgacttg tcaaactcgt aatgtgaata acgaagctat ttgcagctga
     2941 tcgttctctc tttcttagta agagcaatca aacagctgac tgaataatga aaggaagact
     3001 gtatcgtgaa agaaaatcaa gaagaactag ataaagaaat gacagatgct cagccagaac
     3061 cggaaatcga tgtcgaagct gcagaagatt cgggtatttc agaagctgaa gcggaagaat
     3121 tcgaaacagc gaaattaaaa gctgagttag aagaaatgga agataaatac ttgcgggctc
     3181 gtgctgaaat cgcaaatatg gcaaatcgag gaaaaaatga gcgtgagcaa ttacaaaaat
     3241 accgttcaca agatttagcc aaaaaattac ttccatcaat tgacaacctg gaacgtgcat
     3301 tagccacaga agtgtcagat gatcaagggg caggtttgaa aaaaggcgtg gaaatggtct
     3361 tggaaagttt gcgtaacgca ctggaagaag aaggaattga aaaaatccct gctaaaggag
     3421 aagcctttga tcctaatctt catcaagcag tacaaactgt tccagcgact gaagatacac
     3481 cagccgatac gattgtagaa gtactacaag aaggctacaa attacatgat cgcgtgttaa
     3541 gaccaactat ggtcattgtt gcacaataat tgtgcggata taaaacttac actttcaatt
     3601 aaagaaggag acgttaatta tgagtaaaat tatcggtatt gacttaggta cgactaactc
     3661 tgcagtagca gtattggaag gaaacgaagc aaaaatcatc gctaacccag aaggaaaccg
     3721 tacaacacct tctgttgtat cattcaaaaa tggagaaatc caagtagggg aagttgcaaa
     3781 acgtcaagct gtgacaaacc caaatacgat ttcatcaatc aaacgccata tcggtgaagc
     3841 aggttacaaa gtagatgtag aaggtaaatc atatacacca caagaaatct cagcaatgat
     3901 ccttcaatat atcaaaggat tcgcagaaga ctatttaggt gaaaaagtcg aaaaagcagt
     3961 tatcactgtg ccagcttact ttaatgatgc acaacgtcaa gcaacaaaag atgctggtaa
     4021 gatcgctggt ctagaagtag aacgtatcgt caatgaacca actgcagctg ctcttgccta
     4081 tggtttagat aaaacagatc gcgacgaaaa aatcttagta ttcgaccttg gtggcggtac
     4141 ttttgacgta tctatccttg aactaggaga cggtgtcttt gatgtattgt caacagctgg
     4201 cgacaaccat ctaggtgggg acgactttga taacaaaatc atcgactaca tggtagcaga
     4261 attcaaaaaa gaaaacagca ttgacttgtc tcaagacaaa atggctttac aacgcttgaa
     4321 agacgctgct gaaaaagcga aaaaagattt atctggcgta tcaagcacac aaatcagctt
     4381 gccatttatc acagcaggag aatctggacc attgcatttg gaaatgacat tgacacgtgc
     4441 aaaatttgat gaattaacag ctgacttagt agaacgtaca aaagtaccag tacgtcaagc
     4501 gttaaaagat gcaggtctgt cacaatcaga aatcgacgaa gttattttag tcggtggatc
     4561 tactcgtatc cctgcagttg tagaagcagt acgtaaagaa acaggaaaag aaccaaacaa
     4621 atcagttaac cctgatgaag tagttgctat gggtgctgct atccaaggtg gcgtgatcac
     4681 tggtgatgtg aaagatgttg ttttacttga cgttacacca ttgtcattag gtatcgaaac
     4741 aatgggtgga gtgttcacta aattgattga ccgcaataca acgattccaa caagtaaatc
     4801 acaagtattc tcaactgcgg cagataatca accagcagta gacatccatg tattacaagg
     4861 tgaacgccca atggctgctg acaacaaaac tctaggaaga ttccaattga cagatattcc
     4921 agcagctcct cgtggtattc cacaaatcga agtaacattc gatattgata aaaacgggat
     4981 tgtaaatgta tctgcgaaag atctaggtac acaaaaagaa caaaaaatca cgatcaaatc
     5041 ttcttcaggt ctaacagacg aagaaatcga acgtatggtg aaagatgcag aagcaaacgc
     5101 tgaagcagat aaagctcgta aagaggaagt tgacctacgt aatgatgtag acgcattgct
     5161 attctctgtg gacaaaacat tgaaagaatt agaaggcaaa gtggacgaag aagaagtgaa
     5221 aaaagcagaa acagctcgtg acgaattaaa agccgctgtt gaagcaaaca acatcgagga
     5281 aatgaaaact aaacgcgatg cattgaacga aatcgttcaa aacttgactg ttaaactata
     5341 cgaacaagct gcacaacaac aagctcaaga aaatccagaa gcagctcaag gcggagcaga
     5401 tgatgtcgtt gatgctgatt ttgaagaagt cgatggcgac gataaataat cagttcactg
     5461 aatttcgaga gaaggccaaa gttgctactt tggctttttc ttttgaaaaa tagtttgaag
     5521 tatcggtttt tgtttcaaac aaaatatgat atgataacaa gcgacgtgaa aatgatgaga
     5581 accaatagga ggaaagccta tggcaacaaa acgtgattat tatgaagtct tagggctctc
     5641 aaaaggagca tcagaagatg aaataaagaa agcttatcgt aagctttcga aaaaatacca
     5701 tcctgacatc aataaagaac ctgatgcgga agaaaaattc aaagaagtat cagaagcata
     5761 tgagatttta agtgacccgc aaaaacgcgc tgcatacgac caatacgggc atgcaggcac
     5821 agatccaaat tacggtgctg gtggagctgg tggcggattt ggtggattcg gcggcttctc
     5881 tggtggcggc ttcggtggtt ttgaagatat cttcgactct ttctttggag gaggcggtcg
     5941 gtcagtcgat ccaaatgcgc ctcgtcaagg tgctgatttg caatatacgg tcgacttgtc
     6001 ttttgaagaa gcgatttttg gtgtagaaaa agaaatcaag tataatcggg aagaaatctg
     6061 tcatacttgt ggtggaaacg gtgcaaaacc aggcacacaa ccaactactt gtcataaatg
     6121 tcatggtgca ggtacgatca atgtagaacg ccaaacgcca cttggacgag tgatgagccg
     6181 tcaaacttgt gatgtttgtc atggcacagg gaaagaaatc aaagaacctt gtccaacctg
     6241 tcatggctca ggacatgaga agaaagcaca ttcagttaaa gtgaatgtac cagctggtgt
     6301 agaagaagga caacaaatgc gtttagctgg tcaaggagaa gctggagaaa acggcggacc
     6361 atttggtgat ttgtatgtgg tttttcgtgt agaagaaagt gatatctttg atcgtgaagg
     6421 ttctgaaatt tactatgaat tgcctttaag ttttgtacaa gcagctttgg gggatgaagt
     6481 ccaagtacca actgtccatg gagatgtgaa attgaagatc cctgctggaa cacagacagg
     6541 tacgaacttc cgtttacgtg gaaaaggcgc tccgaagtta cgtggtggtg caacaggcga
     6601 ccaacatgtc aaagtaaaac tgatcacacc gaaaaatcta aatgaacaac aaagagaagc
     6661 attacgtgca ttcgctgaag ctggcggcat caaagttgaa gaacagcaag aagacggttt
     6721 cttcgataag tttaaagatg cttttggcgg gaaaaagcgg aagtagtttt tctagtaaaa
     6781 atataaagta gacttgccca aattttctat ttctagtaaa aacgctaagt cattaataat
     6841 taaaaaccag acgaacaatc atacgttcgc ctgg
//
LOCUS       DAYYAJ010000139         6756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000139 DAYYAJ010000000
VERSION     DAYYAJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.139"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHATZ_002591"
     CDS             <1..615
                     /locus_tag="ACHATZ_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8405059.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHATZ_002592"
     CDS             612..1385
                     /locus_tag="ACHATZ_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8405060.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHATZ_002593"
     CDS             1382..1780
                     /locus_tag="ACHATZ_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8405061.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHATZ_002594"
     CDS             1965..2636
                     /locus_tag="ACHATZ_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405062.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHATZ_002595"
     CDS             2695..3135
                     /locus_tag="ACHATZ_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405063.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHATZ_002596"
     CDS             3147..3815
                     /locus_tag="ACHATZ_002596"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8405064.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHATZ_002597"
     CDS             3879..4502
                     /locus_tag="ACHATZ_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8405065.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHATZ_002598"
     CDS             4558..4944
                     /locus_tag="ACHATZ_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8405066.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHATZ_002599"
     CDS             5157..6548
                     /locus_tag="ACHATZ_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8405067.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2155 a         1095 c         1499 g         2007 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggtt
//
LOCUS       DAYYAJ010000140         6665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000140 DAYYAJ010000000
VERSION     DAYYAJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.140"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..256
                     /locus_tag="ACHATZ_002600"
     CDS             <1..256
                     /locus_tag="ACHATZ_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405068.1"
                     /translation="LGKKKPKIEHSLETLLRELLLLFPYIKAETDNISFLPFLHQIVF
                     ATSYTTLIFYKSQHFLAFLLLFRNIFLMFSILCFQLSDNS"
     gene            624..1592
                     /locus_tag="ACHATZ_002601"
     CDS             624..1592
                     /locus_tag="ACHATZ_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8405069.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1610..2515
                     /locus_tag="ACHATZ_002602"
     CDS             1610..2515
                     /locus_tag="ACHATZ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8405070.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2512..3318
                     /locus_tag="ACHATZ_002603"
     CDS             2512..3318
                     /locus_tag="ACHATZ_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8405071.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            3475..4443
                     /locus_tag="ACHATZ_002604"
     CDS             3475..4443
                     /locus_tag="ACHATZ_002604"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8405072.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(4760..5284)
                     /locus_tag="ACHATZ_002605"
     CDS             complement(4760..5284)
                     /locus_tag="ACHATZ_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8405073.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            5571..>6665
                     /gene="scm"
                     /locus_tag="ACHATZ_002606"
     CDS             5571..>6665
                     /gene="scm"
                     /locus_tag="ACHATZ_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8405074.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTS"
BASE COUNT         2100 a         1358 c         1293 g         1914 t
ORIGIN      
        1 tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct
       61 acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca
      121 tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc aacacttcct
      181 ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt
      241 atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt
      301 tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac
      361 ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg
      421 acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa
      481 aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc
      541 aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc
      601 ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc
      661 tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac
      721 aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag
      781 gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt
      841 atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag
      901 aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa
      961 caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca
     1021 catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa
     1081 aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata
     1141 acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag
     1201 cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga
     1261 aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg
     1321 cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag
     1381 ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggta
     1441 aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg
     1501 tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca
     1561 tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt
     1621 gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg
     1681 tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc
     1741 tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg
     1801 tcttctaggc tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa
     1861 attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac
     1921 atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg
     1981 ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc
     2041 tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg
     2101 tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa
     2161 gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct tgcttgcgca
     2221 aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc
     2281 tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt
     2341 cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga
     2401 accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca
     2461 gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac
     2521 gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat
     2581 gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga
     2641 aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag
     2701 ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa
     2761 tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa
     2821 gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa
     2881 ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat
     2941 cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg
     3001 atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac
     3061 cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc
     3121 actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc
     3181 atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca
     3241 gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa
     3301 gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc
     3361 ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac
     3421 tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca
     3481 acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag
     3541 ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa
     3601 aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta
     3661 ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa
     3721 acgatgttga cttctgaatc tacgatgaaa gatgtcaaac tggcagcagc tcaaggagat
     3781 tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg
     3841 ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa
     3901 cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt
     3961 gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga
     4021 aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt
     4081 catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat
     4141 ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag
     4201 ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg
     4261 gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga
     4321 acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca
     4381 aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa
     4441 taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc
     4501 tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt
     4561 attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca
     4621 agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta
     4681 tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag
     4741 ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct
     4801 gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg
     4861 gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt
     4921 aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag
     4981 cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg
     5041 tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat
     5101 cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga
     5161 tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa
     5221 acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat
     5281 ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct aggataccta
     5341 cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt
     5401 tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga
     5461 acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag
     5521 gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa
     5581 gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg
     5641 tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta
     5701 actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg
     5761 tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg
     5821 gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt
     5881 ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg
     5941 gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg
     6001 atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg
     6061 agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta
     6121 aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt
     6181 tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc
     6241 cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt
     6301 ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag
     6361 atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca
     6421 gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc
     6481 aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc
     6541 ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag
     6601 atacatctac cacaagtgat agctcagatt catcaacttc aagcgacagt acagatacta
     6661 cctct
//
LOCUS       DAYYAJ010000141         6649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000141 DAYYAJ010000000
VERSION     DAYYAJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.141"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..770)
                     /locus_tag="ACHATZ_002607"
     CDS             complement(<1..770)
                     /locus_tag="ACHATZ_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8405075.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRN"
     gene            complement(878..1180)
                     /locus_tag="ACHATZ_002608"
                     /pseudo
     CDS             complement(878..1180)
                     /locus_tag="ACHATZ_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            1273..1622
                     /locus_tag="ACHATZ_002609"
                     /pseudo
     CDS             1273..1622
                     /locus_tag="ACHATZ_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1894..2184)
                     /locus_tag="ACHATZ_002610"
     CDS             complement(1894..2184)
                     /locus_tag="ACHATZ_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405076.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2586..2822
                     /locus_tag="ACHATZ_002611"
     CDS             2586..2822
                     /locus_tag="ACHATZ_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405077.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            2829..4781
                     /locus_tag="ACHATZ_002612"
     CDS             2829..4781
                     /locus_tag="ACHATZ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8405078.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4853..5350)
                     /gene="dfrG"
                     /locus_tag="ACHATZ_002613"
     CDS             complement(4853..5350)
                     /gene="dfrG"
                     /locus_tag="ACHATZ_002613"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8405079.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(5677..6369)
                     /locus_tag="ACHATZ_002614"
     CDS             complement(5677..6369)
                     /locus_tag="ACHATZ_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8405080.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2141 a         1147 c         1076 g         2285 t
ORIGIN      
        1 atgttacgag atacatggat acaacaatgc tgatattttg ctttaggata aatttgatgg
       61 atagtatctt tcatgccttt taagccgtcc gtaataaaaa gcaagacttc ttgaactcct
      121 ctggagttaa tatcctgtag cagctcattc caaacgtatg ttgattcagt tggagcaatc
      181 gcataactca gtacttcttt agtgccgtct tctcgtatac caatggcaat ataaatcgct
      241 tctttggata cggtttgacg ttttagtgga atgtaagtag cgtccataaa aatagcgaca
      301 tacttatcat ttaaggctct ggatttaaag gcatttactt cttcagtcag aactttagtc
      361 atgttggaca tggtttgtgg agtatagtga tgaccgtaca ttttttcgat caaatcagca
      421 atttcagaca tcgtaacacc tttttcgaat aaatggataa tagtggtttc caatgtatcg
      481 tttgttcttt tgtaggctgg taaagtttgt tgtttaaact caccattacg atctctaggt
      541 atttccaatg ttaattcacc atattcggtt ttgattgatc gaaagtaaga accgtttctc
      601 gaattacctg aattaaaacc agtgcgatca tatttttcgt aatctaaaaa agccgttaat
      661 tcagtccgta ggagtgtgtt tatcgctttt tctaagtgcg aacggaataa ttcatttaaa
      721 tcgcctttag tgactagagt ttgcacaatt tctgtagtaa aatcattcat agggaagtcc
      781 tcttttctgt gaattggttg tcgttaactt tattctacag aagggacttc cttttttgta
      841 tggatttttt catttacaca aaatatttta tactctcaac atacttgcaa agtaatacta
      901 caattcctaa cataatcgta gaatttaata atttccatag cataaagtgt ttagaaaaga
      961 acattaagaa tagttctata ataattcttg aactccatgt cttataatgt tctatcgaag
     1021 cacccaaata gccaagttca tctaatctac ttgcataaac tacgtcatcc gctctcatag
     1081 tcggcatata catattaaag agtaaaaaga taccccaaat ccctattagt aacaagatca
     1141 gttctttgtt attcgttatt tttctattat gtttgttcat ttttatactc cttcttatat
     1201 ttcctaatag agtataacat agctttaagc attacttaaa gataacttaa aagttatctt
     1261 ttaaatactt aattgttaaa taccttattt gatgcaaaag aaatctacga tagtcaacaa
     1321 aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg attgcagtaa
     1381 tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt aaaattgacg
     1441 aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact gtcgaaccag
     1501 gacgttctcg tgtgaaaggc gaggcttctt caatttactt ttacgactat gataatcacc
     1561 tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat tccactacct
     1621 gagactagta atgactagtc tctttttatg gcctctgatc cccccattga gcgaatacta
     1681 atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg agtttggaaa
     1741 gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt gaggttcttg
     1801 tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca tccatctttc caaaacaagc
     1861 ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat cttgtcggac
     1921 cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt tccctaacac
     1981 atgataggat ctcacaacaa attgctttct attattataa taacctgtaa cgactaactt
     2041 catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca aacaattgac
     2101 cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac ggatcttatc
     2161 tactgttcca tattttttct ccataacctt cacctcaata ccattatacg aacatacatt
     2221 ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt acctttctta
     2281 aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa gaggcagcag
     2341 tatagattta agtagttcag ttttggagta caaaaagtga aaatgtaaac ctaaatgagt
     2401 atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa
     2461 tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa ataattattg
     2521 taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt
     2581 ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat tgggaaatat
     2641 cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt actgaaagcg
     2701 aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt gaatggattg
     2761 ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat agagtgggtt
     2821 gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt gtagatgtta
     2881 aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat ttattaactc
     2941 caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct tttaaatgga
     3001 acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt tgtaattttg
     3061 ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat aaaatgactg
     3121 gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct attggcagag
     3181 gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt gaagaaatta
     3241 aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa tttcataaag
     3301 aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg
     3361 ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag ttcactaatg
     3421 ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc attcttccaa
     3481 tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat attttaggaa
     3541 ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt ttggagtttt
     3601 tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg tggcttaata
     3661 cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat
     3721 atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct gaggtcttat
     3781 caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg gatgaattaa
     3841 atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt aaaaatgata
     3901 ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa aataggaatg
     3961 cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat ggtttaaatg
     4021 ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg gttaatgccg
     4081 acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt tatagagtat
     4141 tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt aacaagacga
     4201 aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg ctcgattggg
     4261 gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat tatctaactg
     4321 aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga gaaagatatc
     4381 gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa ggattttatt
     4441 cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca aagatgctat
     4501 taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt tatatatcat
     4561 ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt tacgctaact
     4621 tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac aacttttgtc
     4681 gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta gggattacag
     4741 ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag cttggagtaa
     4801 tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc cgttaactca
     4861 ataagttttt tctttcatat acatgaaaat agtagttata cggattttta tcattcttta
     4921 tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga aaaaaagtat
     4981 ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa gggaaaaaca
     5041 aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta cataactcaa
     5101 aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc ttatctctcg
     5161 tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg ttcttcctac
     5221 ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc ttgggaatcc
     5281 tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca gcaatcaaag
     5341 aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc ttatttctat
     5401 caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt tctataagtt
     5461 atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt caattgcatt
     5521 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
     5581 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttagtatag attactgttg
     5641 cttctttact aatttaggtt tacttgttaa gaaagattat ttacgcaaat gttcttcaac
     5701 aatttgctga gatttatcag ctgtatcttc cacttcatct tttgcttttt ctaacttttg
     5761 aatatattgt ttccagcttg agttttcagc agttgcttcg tctaatttct tttgtgtatc
     5821 tgttaactga gtttctacat ttgttttttc ttgtgtcagt gttgcgattt gttgattcaa
     5881 accatttaca gtattttgta attctgaaag ttttgcttgt gcttcttgta gtcttgctgt
     5941 ccaatctgca ttcagatgat tgatttcatc atttttttgg ttgatctcat ctagtttgac
     6001 attgatttca ttttgtttgc tttgtagttg tgcatccttg ctgactaatt gactttctaa
     6061 gttttgtttt tctgctgccg attgattttt ttggttttct aattcattta cttttgtttg
     6121 gtattcttgg acatctgcct ttgaatcttg caatgtctgt tctttttgtg ctagtcgagc
     6181 ggtaagatct gtaatcgctt gctctttatc tgtcaatgta tgatcaagct gtgtcagatt
     6241 ctcattgatt gtatcaatat ctgtctctcc accccacaaa ataatttcat ttgcgtaccg
     6301 cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca atcccaccag taataaattt
     6361 ctttttcatt tgcatagctc cttaatccaa tacttttaca tgctaaaagt aagtgatctc
     6421 aaacacaatg tcaagcgttt ttaaattaca tatgtaattt taatataaat actatcaaat
     6481 actcatttaa tcctcctttt ttatctattt aaaaaatctt ttttatagag aaaaatgcag
     6541 gttaatactt tcttaaataa atatttatgg tgaacaaaaa caaaaaagca agttttatta
     6601 aacgtaaccc ttttcatttc atagattact gtatttatta caaaaaacg
//
LOCUS       DAYYAJ010000142         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000142 DAYYAJ010000000
VERSION     DAYYAJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.142"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHATZ_002615"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHATZ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHATZ_002616"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHATZ_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8405081.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHATZ_002617"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHATZ_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHATZ_002618"
     CDS             complement(2143..2373)
                     /locus_tag="ACHATZ_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8405082.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHATZ_002619"
     CDS             complement(2603..3421)
                     /locus_tag="ACHATZ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8405083.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHATZ_002620"
     CDS             3582..4271
                     /locus_tag="ACHATZ_002620"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8405084.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHATZ_002621"
     CDS             4285..5787
                     /locus_tag="ACHATZ_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8405085.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHATZ_002622"
     CDS             5800..6276
                     /locus_tag="ACHATZ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8405086.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHATZ_002623"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHATZ_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYAJ010000143         6594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000143 DAYYAJ010000000
VERSION     DAYYAJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.143"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..979)
                     /locus_tag="ACHATZ_002624"
     CDS             complement(32..979)
                     /locus_tag="ACHATZ_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8405087.1"
                     /translation="MSERISYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1157..1960)
                     /locus_tag="ACHATZ_002625"
     CDS             complement(1157..1960)
                     /locus_tag="ACHATZ_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8405088.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2106..3020
                     /locus_tag="ACHATZ_002626"
     CDS             2106..3020
                     /locus_tag="ACHATZ_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8405089.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3068..3538
                     /locus_tag="ACHATZ_002627"
     CDS             3068..3538
                     /locus_tag="ACHATZ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8405090.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3623..4231
                     /locus_tag="ACHATZ_002628"
     CDS             3623..4231
                     /locus_tag="ACHATZ_002628"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8405091.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4365..4565
                     /locus_tag="ACHATZ_002629"
     CDS             4365..4565
                     /locus_tag="ACHATZ_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8405092.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            5319..6545
                     /locus_tag="ACHATZ_002630"
     CDS             5319..6545
                     /locus_tag="ACHATZ_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8405093.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2228 a         1001 c         1317 g         2048 t
ORIGIN      
        1 ttttttcttt tgaaaatatt gttttcagct attaatatcc tgcagacaat tttacttgat
       61 tggtttgaag ctcatgggtg gcaaagtagc ttttcagatt ggttaaaaaa atagccatta
      121 atttcttttg aaaatcaggt gtcaaacctg caatgtggga agtaatcagt acattgtcca
      181 ttttccaaag aggatgatct tgtggaagag gctcttcctc aaaaacgtct aatgctgcaa
      241 aggaaagttg ctggttattt aaagcctgca ccagatcttg tgtcttaaca gaagcaccac
      301 ggccaacatt gatgaataca gattttggat caaagtgttc aaacatatgc tgatcaaaga
      361 gcccagttgt ttcctccgtt agaggtaaga tattgactac gaaatctact tcaggaagaa
      421 cttcctttag ttgatttaaa gaaaaagtct tttccattcc ttctgctgga tgaccggatg
      481 tattgattcc atagcagttc atgtctaatg ctttgcattt ttttgcaatc atttttccaa
      541 tatggccagt tccaatgatt agtatttttt tcccttggat agattggtaa tggaccgatg
      601 aacctaacca tttcatgtca ctctgtgcct tttgtgcttg aaaaagacca cgagaatacc
      661 caaggatgat tcctaagaca tgctccgtaa tcgattcact gtggatgcca ctaccatttg
      721 aaagtaaaat atgtttcttt gaaaaagtct caaggggtag ctgatcaaca ccagctgaga
      781 tagattggac ccatttcaag gtagaagagg ctaatagttt ttcttggtag tctgagttcc
      841 agcccaaact gatctctaca ttttgtagat cctctggttg taattctgtt ttaaagatat
      901 aaccaggcgc tatagtatgg atttcttcga taaattcatc acgaaatgga cgatctgcat
      961 aactgattct ttctgacata gttaatttcc cgcctttctt tactctaagt gtagcgcata
     1021 caagaagaac tttgaagcgc cttgcttgtt ttttgagaaa gaaatatttt tcaaataaaa
     1081 aaggggctgt gacaaaaaaa tgtcacaacc tctaaaaggg tctattgaag tagaaataac
     1141 gagttgtctt tatttcttaa ttgatcgata tttttgccca tcctttgaca gcttgagcaa
     1201 aaataaagat tcctgcaaaa gcaatgaagg cgctagtgtc aaaagaagga agaaagacca
     1261 tcaagattgt tgcaattata gtaggcaatg aaattgaata gatcaatgtt tttaaacaat
     1321 caagaaaagt agcttttgtt agtcgaaagc gtgcatagag aaaagcagca atgttggtaa
     1381 atagcaaagt gatcagcaga ttcagaaaac taggataaat agaaaatata aatgtcagga
     1441 cttttatcca aagtggtata gatgcttcac ttaaaaaaga acggagctgc ttacctgtta
     1501 agttttttaa agattcattg gtgtagggaa gttctaattg gttattgttc aacaaagcag
     1561 aagtcgtgcc tgtattagga agagctatga taagtttgtt ttttaacatc ccgacactaa
     1621 caaaattccc catcaaatca gtagagatgt cctgttctgt tcgttttccc tctggatcaa
     1681 atgtgaagat aatggagttt gtttggtaaa tgaatccttc tttttcttcg gtttgaagct
     1741 gcccgttttg gattgtaaaa tcaggaatgc gagtagcaat cttttgccca tccaattgaa
     1801 tgttgtttaa gacttgaaaa acttgaaaag agattggcaa agccataatt atactcaaaa
     1861 ctaaaagata gacaatggct ttccaaaatg ggacatttct cgcctccttt aggtctttga
     1921 atcgtataaa ggaactgata attaattgtt ttgttgtcat aagtactcct catctagtag
     1981 tgtcatcact tgcaattcta gcgaagtatc aagtgaaata caagtgcttt aaggaaacag
     2041 ctgaatgatt ggtgaaatat caaaaaaatg ttattcttgt ctaaagacta tccaatataa
     2101 ataaaatgga ggaaggaata gtgcctacac agccacagag atcatcaaaa aaactatttg
     2161 gagtagtggt tttgaatgcg gtgatcactt tatcagaatt tattggaggg attatgtcag
     2221 ggagtttgtc tcttgtttca gatgctttcc ataaccttag tgatacattg gcaattattt
     2281 tttcttacgg ggcacaaaaa atggcccgaa aagaagcaaa taaaaaacgt acgtatggct
     2341 accagcgttt agaaatattg agtgctttca tcaactcatt catattgatc atattatcct
     2401 tgtttttaac cgttgaagca ttcaagcggt tcacttcacc agaaaagata aatagtcatt
     2461 tgatgctaac agtagcagtt attggattgc tcgcgaattt gttctcagta ttattattac
     2521 gacaagaagc agatgaaagt ttgaatataa aatctagtta tttgcatttg ttaagtgata
     2581 cactgtcttc ggtaagtgtg attatcggag caattttgat ccgttttttc gatatctact
     2641 ggatcgatcc agttattaca ctgatcattt cgatatatat attgatagaa gcgataatgg
     2701 tgatcaaaaa agctgccgct attttgatac aaagtgcacc tgctatcgac tatgaaaaaa
     2761 tggaacaaga aattgaagca atcgatgggg taaaggatgt tcatcatgtc catatttggc
     2821 agtatagcga aaagataatt attttcgacg gacatattga ttttgaagat cagctactaa
     2881 gcgagattga aaagatatat cctcgtataa tttctttatt aaaattaaaa tatggtatta
     2941 cacatgtgac gatccaagca gagacaaagg caaaggatca aaaaaaatta atttttttaa
     3001 ataaagatga aagttattga aatatagagt atgataaata agaattttta ataaggtagg
     3061 ttggctcatg aagcagtatc gacagttaaa aggatattta gaagcaaaag gatattggga
     3121 gatactagtc tatctctttt ttggtggttt ggctacgatt gtaaattttg ttagttttgc
     3181 tctcgcacgc caatattttg atttaagtat ggccttatcc aattctattt cgtggttttg
     3241 ttctgtattg tttgcttttg taacgaacaa actttgggta ttccattcaa aatcaccgaa
     3301 ttttacacat gcacttattg aatgcggtaa gttcttcttc taccgtatcc tttcttacgg
     3361 attggacatg ggggcaatgg tattgttgat caatgttatg aacagcaatg agtatgtggc
     3421 gaaaatcatc actcaaatta ttgttatcct tgcgaactat attttcagca agttgtttat
     3481 ttttaaagaa actgaggttt ttgaagaaga agtaggtcaa agtgaaaaag acgactaggc
     3541 ataacaattg aagaatttcc ttctctttgt tactctttgt aatgtagaca ttattaagaa
     3601 aacattgagg aggaattttt ttatgactta cacattacca gatctacctt atgcatatga
     3661 cgcattggaa ccttatattg acgaagaaac gatgcatctg catcatgata agcatcacaa
     3721 tacttatgtg acgaatttaa attcagcaat tgagaaatac ccagaattag gcgaaaaaac
     3781 aatagaagaa ttattatctg atatggacgc tattccaaca gatatcaaga cagctgtacg
     3841 taacaatggt ggcggacatg ctaaccattc atttttctgg gaaatcatgg caccaaatgc
     3901 tggtggcgaa cctacaggag aaataaaaga agcgattaat gaagcttttg gtgatttttc
     3961 ttcttttaaa gaagaattca aaaaagcagc cgctggacga tttggttctg gatgggcttg
     4021 gcttgtaatg gaaaatggaa aattagctat tacctctact gcaaatcaag attctccatt
     4081 gatggaagga aagacaccaa ttctaggttt ggacgtttgg gaacatgctt attatttgaa
     4141 atataaaaat gtccgaccag attatattgc agcattctgg aatgttatta attgggatga
     4201 agtaaacaaa cgtttcgaag cagcaaaata accatttcct ccgtcatatc agtcttaaaa
     4261 ataatttgga aaataaaaga gcgaaagctt gctagcgttt tctaaattgg gtataatgtt
     4321 cttgcaaggt tacttgcaag aacttttttg gaggatatac atttatggaa cacggaacgg
     4381 ttaaatggtt caacaatgaa aaaggatttg ggtttattac agtagacggc ggagatgacg
     4441 tatttgttca tttctcagca attcaaggag acggcttcaa gagcctagaa gaaggtcaag
     4501 aagtagaatt cacgattgtt gaaggtgcac gcggacctca agctgctgaa gtgactaaat
     4561 tataataatt tgttgaataa agacacgact tcttagagag tcgtgtcttt ttgtctattt
     4621 atgaagtttt tagatttttt ggctaagtaa atgcatttgt tcacttgaca tgtcctaact
     4681 aattatcaag caaggaacat tttcttcgtt agtaatggat tttaaagaaa tttcagtttt
     4741 tggatgttga ataaaagacc atttgtgaaa ccactagtat acatactcat taccatgtca
     4801 attctattac tttaaaagaa aatcgtttta ctttgaagag gagcatatct atccattaaa
     4861 agatgatggt aatgcaatca ctagttcgag tcattttaag agccatgcag tgagctggaa
     4921 ataaaacgaa tagaagatgg gtttaacaaa aggaccagtc ataggaagaa attgtcgtga
     4981 aatcgaatga taatctgaac ggtaggcaaa gactgttaaa aagatgggaa aggtatgtgc
     5041 gatcatttta ctgcacgatt atttggaaaa taccttgttg ttcgcttttt ctggaaaaaa
     5101 tacataaaaa aagataaaac gattgcgaac tgcgtgtatt cggtctttcg atctttttca
     5161 cggtatggta tgatagttaa gaattttgag aaattttaaa aaataataat cgttacacaa
     5221 aaatgttttt taaagttttc ttatgattaa aaagtgaatt tgctgattga acagttatat
     5281 gagacaatat tttagaaaat gtgaaacaga ggtgtattat gaagcgatgg caaaaagttc
     5341 tattaacctt gttaggggta ttcgttttgc tgattgccgg attatcagca tacggaatca
     5401 gatttatggg agaagcgagc caaacagtaa acaaaatttc taaaaactct aatcgaatat
     5461 cctccaaacg ttctgagaga gtaagcattg atgacaaaga acctttttcg gttttgctat
     5521 taggtttaga taccggtgga ttaggaagaa cggaacaagg acgttctgat acgatgatgg
     5581 tagtaacagt taatccgaaa caaaaaaaat caacgatcat cagtttggac cgagatattt
     5641 atacgaatat cgtaggttat ggaacggtag ataaattaaa tcatgcctac gcatttggtg
     5701 gtgtcgaaat ggccatggat tcaattgagc aactgctcga tattcctatt gatcattatg
     5761 taacgatcaa tttggatggc atggaagatt tgatcaatgc ggtcggtggc atcaaagtga
     5821 ataataaaat tgactttact ttggatggtg tccatgtacc agtaggtgaa caaacattga
     5881 atggagaaaa aggtttagcc tattctcgaa tgcgacacga agatccagaa ggcgatatcg
     5941 gtcgacaacg tcgtcagcgg gaagtagtga cgaaaatcgt caataaagtt ttaagtttag
     6001 atggcgtaag caattataga aaaattttaa aagcagttga gaacaatgtg acgacggact
     6061 tagattggga tgacatgctt gatgtagcga cgaactacac cccggcgttt gaaacaatca
     6121 aacaagacca gttgcaaggc gaaggacaaa tgatcaatga tatctactat caaattttag
     6181 gaaaaaatga tcttctttcg atccaaaatg aactgaagaa acaattaaat ataaaaacaa
     6241 gcgatacatt acctaatttg aaaaatgaga atgcttcaat gatgttcttt gatgattcgg
     6301 aagatggcga ttcgacaaac ggtgatggat cgaatagtga agggtacacc gattattctt
     6361 cctattcaga aaacacatat gatcagtacc aagatccgaa tacctattat cagcaaacag
     6421 aagatacaag tcagtactat acaccaagtc aagatactca aagttatgat ccttacgctg
     6481 gacaggaaca aaatggttat ggttatgacc agaataatga gtatggaaca acaagtgggt
     6541 actagaaaaa aatagtttgg ttaataaaga gtgccaccta aattagacaa aaaa
//
LOCUS       DAYYAJ010000144         6590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000144 DAYYAJ010000000
VERSION     DAYYAJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.144"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHATZ_002631"
     CDS             complement(99..1229)
                     /locus_tag="ACHATZ_002631"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8405094.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHATZ_002632"
     CDS             complement(1878..3080)
                     /locus_tag="ACHATZ_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8405095.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHATZ_002633"
     CDS             complement(3140..3343)
                     /locus_tag="ACHATZ_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8405096.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHATZ_002634"
     CDS             complement(3485..4552)
                     /locus_tag="ACHATZ_002634"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8405097.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHATZ_002635"
     CDS             complement(4610..4717)
                     /locus_tag="ACHATZ_002635"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405098.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHATZ_002636"
     CDS             4880..5692
                     /locus_tag="ACHATZ_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405099.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHATZ_002637"
     CDS             complement(6066..6257)
                     /locus_tag="ACHATZ_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405100.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1998 a         1331 c         1030 g         2231 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag
//
LOCUS       DAYYAJ010000145         6577 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000145 DAYYAJ010000000
VERSION     DAYYAJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.145"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHATZ_002638"
     CDS             complement(1273..1938)
                     /locus_tag="ACHATZ_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405101.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHATZ_002639"
     CDS             2064..4100
                     /locus_tag="ACHATZ_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8405102.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHATZ_002640"
     CDS             4104..4742
                     /locus_tag="ACHATZ_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8405103.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHATZ_002641"
     CDS             4770..5198
                     /locus_tag="ACHATZ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405104.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHATZ_002642"
     CDS             5655..6566
                     /locus_tag="ACHATZ_002642"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8405105.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2256 a          998 c         1218 g         2105 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaat
//
LOCUS       DAYYAJ010000146         6537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000146 DAYYAJ010000000
VERSION     DAYYAJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.146"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..641
                     /locus_tag="ACHATZ_002643"
     CDS             <1..641
                     /locus_tag="ACHATZ_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8405106.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(727..1821)
                     /locus_tag="ACHATZ_002644"
     CDS             complement(727..1821)
                     /locus_tag="ACHATZ_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8405107.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            2010..2771
                     /locus_tag="ACHATZ_002645"
     CDS             2010..2771
                     /locus_tag="ACHATZ_002645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8405108.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="ACHATZ_002646"
     CDS             complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="ACHATZ_002646"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8405109.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3546..4301
                     /gene="fabI"
                     /locus_tag="ACHATZ_002647"
     CDS             3546..4301
                     /gene="fabI"
                     /locus_tag="ACHATZ_002647"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8405110.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(4411..5463)
                     /locus_tag="ACHATZ_002648"
     CDS             complement(4411..5463)
                     /locus_tag="ACHATZ_002648"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8405111.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            5589..6509
                     /locus_tag="ACHATZ_002649"
     CDS             5589..6509
                     /locus_tag="ACHATZ_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8405112.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         2013 a         1300 c         1161 g         2063 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa
      661 aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact
      721 aattatctat tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag
      781 gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat
      841 atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag
      901 tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta
      961 tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag
     1021 gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa
     1081 tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta
     1141 cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt
     1201 cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg
     1261 gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa
     1321 tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa
     1381 attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta
     1441 attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt
     1501 tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg
     1561 gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc
     1621 aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag
     1681 aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata
     1741 cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat
     1801 cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca
     1861 cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa
     1921 atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata
     1981 acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta
     2041 gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta
     2101 gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg
     2161 tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc
     2221 tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac
     2281 caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt
     2341 tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg
     2401 gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac ctttataata
     2461 taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct
     2521 ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag
     2581 caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct
     2641 tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata
     2701 gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa
     2761 aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca
     2821 ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt
     2881 tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca
     2941 tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa
     3001 tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca
     3061 ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct
     3121 tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct
     3181 tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct
     3241 aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact
     3301 tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa ttgccatttt
     3361 cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt ttttaattta
     3421 agaaaaaata gtttgacaat caaaacattt tgactaataa ttaagaaatc aagaagttat
     3481 tttagctatt attctctatt tttttacgac tatcaaattt ttatcagaaa caaaggagag
     3541 tcattatgtc atttctagaa ggaaaaaaaa tcgtcattat gggcgtcgct aacaaaaaaa
     3601 gcattgcttg gggttgtgct gaagcaatgt tgaaacaagg ggcagaaatt atctatactt
     3661 accaaaatga acgaatgaaa aaatcattag tcaaattggt cggcgaagaa gcattcactg
     3721 tagaatgtga tgtttctagc gacgaaagca tcgaaaaagc aatgaatgcc atcggtgaat
     3781 atgctgggga gatccacggc cttgtccatg cagttgctta cgccaacaaa gaagaattgt
     3841 ccggaaatgt gtctgatatt tccagagacg gctatcaatt agctcaagat atcagcagct
     3901 actctttaat tgctgtatca aaatacgcac aaccgtttct tgcaccaaac agcgggatcg
     3961 ttagcatgac ttatctaggt tcagaacgcg caatcccgaa ttataacatg atgggtattg
     4021 cgaaagcttc tcttgaagct gctatccgtt atttggcagc tgagttctcg cctaaaggaa
     4081 ttcgggtcaa tggaatctca gctggggcaa tcaaaacatt agctgtcaca ggtgttaaag
     4141 actatcaaaa actgatccag ttatctgaat ctcgtacgcc tgatcagcaa ggtgtgacga
     4201 tcgaagaagt cggtaatgct tgtgctttct taatcagccc actttccagc ggaatcatcg
     4261 gtgatattat ctacgttgac aaaggtgtcc atctttcata aacttttgtt gcattcttta
     4321 aagtaaaaca tataaaaatc aggaaaaaac tttttaaagt ctcttcctga ttttttcttt
     4381 ttacgttttt ttcaactcat tactttgaaa ttattccacg acttcaatcc agccttctgg
     4441 tgcttctatg tctccaaatt gaatacctgt caattcatca tacaaccgtt ttgttactgg
     4501 tcctacttcc gtttcactat aaaatacatg aagattgtta ttgtgataaa tcccaccgat
     4561 tggagaaata acagccgctg taccacatgc accagcttct gcaaattgat ctagatcatc
     4621 aatgaaaata tctccttcaa ttgcttgcag tcctagtcgt tcttcagcta actgcaacaa
     4681 ggaatatttc gtgatacttg gtaagataga gggcgattgc ggagtgatga attttccgtc
     4741 ttttgtaatt ccaaagaaat ttgctgcacc tacttcttcg attttcgtat gtgtggctgg
     4801 atcaagataa acacaatctg agtaatccag ttcttttgct tctgttcctg gaagtaaact
     4861 cgctgcgtaa tttccgccga cttttgctgc accagtcccc ttaggagcag cacgatcgta
     4921 atcagataca ataaaattcg ttggttttag tccacctttg aaatacgctc ctactggcgt
     4981 acagaagacg acaaataaat attctggagc tggatggaca ccaatgtttt caccgacccc
     5041 aatcagtaaa gggcgcaagt ataatgtagc gcctgttcca tagggcggca caaaatcttc
     5101 atttgctttt accacttgct tgacagcatc aacgaacatc ttttcaggga ccattggcat
     5161 cagcaatcgt ctagcacttt gattcatccg tcgactattt tgttcaggtc gaaagagatt
     5221 gaccttgccg tcttttcgac gataagcttt tagaccttca aaacattgct gaccataatg
     5281 aagcgctgga gagccttcat gaatcgtcag gtaattatct tctgtcaatt ctccttcttc
     5341 ccattgtccg tctttccatt tggcaataaa acgccaagga gttttgatat aagaaaaacc
     5401 tagattgttc caatcgattt gcatatactt accaccttcg ttattttctc cacattttat
     5461 cattatccat attatttgtc attttatttt cagctttttt ccttcattat agttattttt
     5521 gctatttcct tttttgaaaa cttcttggta caatacagaa agaatcaaat tttagaaaag
     5581 aggattttat gttttcatta ttagcaactg ccggtacgac agactcttct ggcgagacat
     5641 tgacagatca agctgtcagc aaagtaaatg cgtttcagcg gttttgggac gggattgatt
     5701 gggacgccat tttggctgcc tttatccaaa aaagtttata tctgattttt ttgatcgtcc
     5761 ttttttggat tcttcaccgt gtgggaaaat atctgattga taagtctttt aatcagtatt
     5821 ctaaaaaagc cattctaaac gagagccgct taaagacttt acatagtctt ttaaatacta
     5881 tttttcatta tacccttggt ttctttttta tctatgcaat tttatctgct atcggtgtgc
     5941 cgatcggatc acttttggct ggtgccggaa ttgcaggtgt ggcaatcggt cttggtgccc
     6001 aagggttcat gagtgatgtt attaccggat tttttattat catggaacaa cagatggatg
     6061 ttggggacta catcaagcta gcaaacttaa cgattgaagg aaccgtcgta tctgttggta
     6121 tccgtaccct tcagctgaaa gcaacggatg gtactgtcca tttcatcccg aatcgaaaca
     6181 tcaccacgat cagcaatctc tctcgtgcaa atatgcaagt acttttagat attcggattg
     6241 ttcctgaaga aggatatgac aaaatctatg agatcattga tcgagtaaat cgtacattag
     6301 ctgaaaaata ccaagaagag ctccaaactg aaccttctat ctttggatta gtggatcttg
     6361 gaaacagcaa ttttgcgatt cgtacggtct gctacgcatt gaacggaaaa caatatgcct
     6421 taaaagaaga gttcttgagc tcctatgtca aagaattgac agcttctggt ttcacgattc
     6481 cgaacagtcc aattgctgtc ggaaaataat ttggacaaac gatgcaacga atctcac
//
LOCUS       DAYYAJ010000147         6387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000147 DAYYAJ010000000
VERSION     DAYYAJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.147"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHATZ_002650"
     CDS             330..1175
                     /locus_tag="ACHATZ_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8405113.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACHATZ_002651"
     CDS             1456..2676
                     /locus_tag="ACHATZ_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8405114.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACHATZ_002652"
     CDS             2673..5393
                     /locus_tag="ACHATZ_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8405115.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACHATZ_002653"
     CDS             5377..6321
                     /locus_tag="ACHATZ_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8405116.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAYYAJ010000148         6282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000148 DAYYAJ010000000
VERSION     DAYYAJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.148"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..285
                     /locus_tag="ACHATZ_002654"
     CDS             76..285
                     /locus_tag="ACHATZ_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8405117.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            278..427
                     /locus_tag="ACHATZ_002655"
     CDS             278..427
                     /locus_tag="ACHATZ_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8405118.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            581..1246
                     /locus_tag="ACHATZ_002656"
     CDS             581..1246
                     /locus_tag="ACHATZ_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8405119.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1530..2615)
                     /gene="xerS"
                     /locus_tag="ACHATZ_002657"
     CDS             complement(1530..2615)
                     /gene="xerS"
                     /locus_tag="ACHATZ_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8405120.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2949..3182
                     /locus_tag="ACHATZ_002658"
     CDS             2949..3182
                     /locus_tag="ACHATZ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8405121.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            3179..3586
                     /locus_tag="ACHATZ_002659"
     CDS             3179..3586
                     /locus_tag="ACHATZ_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8405122.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4723..5241)
                     /locus_tag="ACHATZ_002660"
     CDS             complement(4723..5241)
                     /locus_tag="ACHATZ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8405123.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            5608..5856
                     /locus_tag="ACHATZ_002661"
     CDS             5608..5856
                     /locus_tag="ACHATZ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405124.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         2145 a          976 c         1019 g         2142 t
ORIGIN      
        1 aataaaaata tgcaggaacg ctactatttt tagtagtgtt cttttttata tttgaataaa
       61 aaatgtggtg gttaaatggt ggttataaca aaaaatgaat taatgaaatt aggttatggg
      121 aagtatcagt ctgagagaat catacgacag gcaaaagcaa ttatggcttc aaaaagctat
      181 acctattacg aaaatagaag attaggacta gtacctgttg aatctgtaga agaagtttta
      241 ggtatgaaga tagaggtaga ggaggatttt cagaatcatg gttaatttta aaaaagataa
      301 aaatgggaaa tatcatttta ctgctagttt gggattcgac aaacgaacag ggaaaagagt
      361 acaaaaaaac gtagaggggt taaaacagtt aaagaagaaa aaaagcgtat accgaacttg
      421 tttctaatta tggaaaagaa gcagaaactt ctcaaagtac tatgttgttt gaggagtttt
      481 tttataaatt attcctacct tattatcaag ggaaagtaga agaaagaaca tataaaaatc
      541 ggttatctac aatcaaaaaa acttttctta tttctttaaa atggagttaa gagaaatacg
      601 tccaattgac attcaaaagt ggcaaaataa tttattgagc aaatataaaa attcttatgt
      661 aagaaacatg tatgggattt ttcagatgtc attagatcga gcagttgttt tgggactact
      721 tgaaaaaaat cctgcaaaag ttattggaaa tgtgaagaaa aggaaaacta cagttgattt
      781 ttggacaaaa gaagaattta caaaagttat ttcaactttt tatattgatg atttgtacca
      841 acattttggt tttatttgta tgtggttact ttttatgacc gttatgcgtt tgggagaagc
      901 cactgcatta acttggaaag atattgattt tgaaaataaa aacttgcgtg tgaataaaac
      961 actctattat aagagagcca aaacctatga gtttgtagaa cccgaaacgg cttctagtat
     1021 tcgtacaatc gaactagacg acaatacaat taccttttta aaagcttgga aggagcgtca
     1081 agtttcaata ggaggcatgt cgtttgtact ttcttataat ggaacaccta ctcagaaata
     1141 tacaatccgt catattatga gaagacatgc aacgatcgtt ggtgttcata taatccgcat
     1201 acacggatta aggcatagtc atgcttcttt gcttatatca caataaaaag atgcacttcg
     1261 aattaaagaa aggttaaaac atgctgatgt acaaacgact ttaggaactt atggtcattt
     1321 gtatctaaat tcattgagag agataacaaa tgatcccaca gattatatcc aaactaattt
     1381 ttctaaaatc aaaccgacac ctatttacga taataaaaaa taaatgataa acaaaagtag
     1441 gatcacacca aaagaaacac actagaaaaa ctataataca ggcttttcta gcgtgttgtt
     1501 tcttatttcc gcctatttat acattatact cataaagcat ttaatgcatc gatcgtagcc
     1561 gcattgtcta tatgggtata taaagttgtt gtattggtac ttgaatgtcc caattgttgg
     1621 gcagttaata ccaatgaatt tgtctgttgg tagagtctag tagctaatgt atgacgcagt
     1681 ttgtgagggc tgacttgaat tctaaagcct tttgaatatt ttgctaccat ccgttcgatt
     1741 gcaataccgg atatacgctt ggcttctcct tggtaaattg ctagaaataa agctttttcg
     1801 ttttctgtta ctttatatcg ttctttccga atagctagat aattagctaa atattccatt
     1861 gcgaatgctg ctatattgac agcatctctt ttatttcctt ttctagttac taccacatta
     1921 ttcttatcta aagataggtc atctaaatta atatttataa cctcggagag tctcaatcca
     1981 gatcctaaaa tcagtgcatt gatcgccagg tctctttcta catctcgttg ataatagatc
     2041 aaagctcgtt tactcaaagt atttttatat tcattggcaa tataatctag ataattttgt
     2101 gtatccttat ctagaaataa ttttttctca atggcattgg cacgtgcagc taacgtagtt
     2161 ttatctgtga tggttgctac ttgatccata ggattattgt ctaagtatgg ttttcctaga
     2221 ggattattgg aacttttcga aagatattta aatagaacct ttaatgccgt gatatttcta
     2281 ttgatagtag aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc
     2341 tcttttttta cattttcttt tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca
     2401 taggcagtta actgtcttat cgaggtgata gaagtatcag gaacgataga ctcactcaga
     2461 agccattcat aaaagagctt ataatttttt agatattcta aagtggttcg aggtgattga
     2521 tctttatcct cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt
     2581 gtatctattt tttgatatat ttctgtattt ttcatgtcat tcatcctttc aataagtagg
     2641 ggagaagagt atctctataa ttggactata ccaattatag aatgttttgt caattggtat
     2701 gctctaaaat ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat
     2761 ttaaaataaa tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt
     2821 gaattttctt aattaattat atagaaagaa caatctttct atagagttag acatgagagg
     2881 gactatgctt gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg
     2941 agaagactat ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa
     3001 ctatttcaaa ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag
     3061 atgaagaaaa acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt
     3121 tagcaatatc tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat
     3181 gagtattcat tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa
     3241 atactctcct aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg
     3301 gcaacttcga gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc
     3361 tatcctactt atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc
     3421 tttcttagct acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa
     3481 agatgctgta aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa
     3541 atatgaagtg atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg
     3601 tgtctttata gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta
     3661 taattcaaat ccaactatat agaaaacgta aattttctat atagttggat taagataacg
     3721 actatttatt ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat
     3781 aatcgcaaat ttacccctat actactagtt tgtaagttag attaataaaa atactaaagt
     3841 tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt agataacacg
     3901 aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt
     3961 gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt
     4021 gcagaaatcg ttactgggat taaactaagc aataaaaact aatgttcccc tcattctttg
     4081 aacttctact atttgattta tttattctga taaatgacga tttcaccttc aggatcttca
     4141 aatcccgtat agaatttatt taagatctct tcagtatcag taataggata ggcgataggc
     4201 cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag
     4261 catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat
     4321 ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat
     4381 gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa
     4441 catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata
     4501 tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat
     4561 tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc
     4621 aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac
     4681 aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt
     4741 ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa
     4801 tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc
     4861 agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg
     4921 attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt
     4981 atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc
     5041 atttctttca tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa
     5101 attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat
     5161 agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc
     5221 tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt
     5281 catctataat ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt
     5341 gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat
     5401 gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac
     5461 tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta
     5521 ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca
     5581 caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta
     5641 cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac
     5701 gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc
     5761 gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt
     5821 aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca
     5881 caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta
     5941 atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat
     6001 tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg
     6061 tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt
     6121 aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga
     6181 cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc
     6241 tcatttcaac ttatatgttt tagtgaacta cccccactta gc
//
LOCUS       DAYYAJ010000149         6261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000149 DAYYAJ010000000
VERSION     DAYYAJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.149"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHATZ_002662"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHATZ_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8405125.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHATZ_002663"
     CDS             500..862
                     /locus_tag="ACHATZ_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8405126.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHATZ_002664"
     CDS             914..2596
                     /locus_tag="ACHATZ_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8405127.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHATZ_002665"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHATZ_002665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8405128.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHATZ_002666"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHATZ_002666"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8405129.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHATZ_002667"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHATZ_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8405130.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2079 a         1090 c         1428 g         1664 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat t
//
LOCUS       DAYYAJ010000150         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000150 DAYYAJ010000000
VERSION     DAYYAJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.150"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHATZ_002668"
     CDS             725..811
                     /locus_tag="ACHATZ_002668"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8405131.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHATZ_002669"
     CDS             complement(932..1621)
                     /locus_tag="ACHATZ_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405132.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHATZ_002670"
     CDS             complement(1866..2780)
                     /locus_tag="ACHATZ_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8405133.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHATZ_002671"
     CDS             complement(2762..3142)
                     /locus_tag="ACHATZ_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8405134.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHATZ_002672"
     CDS             complement(3592..3879)
                     /locus_tag="ACHATZ_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8405135.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHATZ_002673"
     CDS             complement(3899..4123)
                     /locus_tag="ACHATZ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8405136.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHATZ_002674"
     CDS             complement(4578..4748)
                     /locus_tag="ACHATZ_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405137.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHATZ_002675"
     CDS             complement(4763..5296)
                     /locus_tag="ACHATZ_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8405138.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHATZ_002676"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHATZ_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8405139.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYAJ010000151         5982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000151 DAYYAJ010000000
VERSION     DAYYAJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.151"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACHATZ_002677"
     CDS             complement(<1..450)
                     /locus_tag="ACHATZ_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8405140.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="ACHATZ_002678"
     CDS             674..1465
                     /locus_tag="ACHATZ_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8405141.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="ACHATZ_002679"
     CDS             1431..2384
                     /locus_tag="ACHATZ_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM8405142.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="ACHATZ_002680"
     CDS             complement(2782..3756)
                     /locus_tag="ACHATZ_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8405143.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="ACHATZ_002681"
     CDS             3871..4305
                     /locus_tag="ACHATZ_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8405144.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="ACHATZ_002682"
     CDS             complement(4430..5452)
                     /locus_tag="ACHATZ_002682"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM8405145.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5982
                     /locus_tag="ACHATZ_002683"
     CDS             5844..>5982
                     /locus_tag="ACHATZ_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM8405146.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PR"
BASE COUNT         1880 a         1128 c         1032 g         1942 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag ag
//
LOCUS       DAYYAJ010000152         5980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000152 DAYYAJ010000000
VERSION     DAYYAJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.152"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(191..676)
                     /locus_tag="ACHATZ_002684"
     CDS             complement(191..676)
                     /locus_tag="ACHATZ_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405147.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(815..1030)
                     /locus_tag="ACHATZ_002685"
     CDS             complement(815..1030)
                     /locus_tag="ACHATZ_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8405148.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1254..2054
                     /locus_tag="ACHATZ_002686"
     CDS             1254..2054
                     /locus_tag="ACHATZ_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8405149.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2073..3941
                     /locus_tag="ACHATZ_002687"
     CDS             2073..3941
                     /locus_tag="ACHATZ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8405150.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3934..4425
                     /locus_tag="ACHATZ_002688"
     CDS             3934..4425
                     /locus_tag="ACHATZ_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8405151.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4413..4967
                     /locus_tag="ACHATZ_002689"
     CDS             4413..4967
                     /locus_tag="ACHATZ_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8405152.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4985..5980
                     /locus_tag="ACHATZ_002690"
     CDS             4985..5980
                     /locus_tag="ACHATZ_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8405153.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2098 a          952 c         1207 g         1723 t
ORIGIN      
        1 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
       61 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
      121 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
      181 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
      241 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
      301 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
      361 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
      421 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
      481 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
      541 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
      601 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
      661 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
      721 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
      781 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
      841 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
      901 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
      961 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     1021 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     1081 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     1141 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     1201 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     1261 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     1321 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     1381 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     1441 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     1501 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     1561 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     1621 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     1681 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     1741 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     1801 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     1861 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     1921 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     1981 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     2041 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     2101 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     2161 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     2221 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     2281 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     2341 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     2401 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     2461 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     2521 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     2581 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     2641 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     2701 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     2761 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     2821 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     2881 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     2941 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     3001 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     3061 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     3121 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     3181 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     3241 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     3301 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     3361 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     3421 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     3481 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     3541 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     3601 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     3661 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     3721 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     3781 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     3841 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     3901 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     3961 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     4021 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     4081 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     4141 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     4201 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     4261 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     4321 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     4381 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     4441 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     4501 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     4561 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     4621 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     4681 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     4741 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     4801 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     4861 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     4921 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     4981 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     5041 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     5101 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     5161 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     5221 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     5281 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     5341 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     5401 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     5461 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     5521 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     5581 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     5641 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     5701 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     5761 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     5821 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     5881 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     5941 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DAYYAJ010000153         5954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000153 DAYYAJ010000000
VERSION     DAYYAJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.153"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHATZ_002691"
     CDS             <1..813
                     /locus_tag="ACHATZ_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8405154.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHATZ_002692"
     CDS             786..1229
                     /locus_tag="ACHATZ_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405155.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACHATZ_002693"
     CDS             2399..3367
                     /locus_tag="ACHATZ_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8405156.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACHATZ_002694"
     CDS             3384..3908
                     /locus_tag="ACHATZ_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8405157.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACHATZ_002695"
     CDS             4284..4565
                     /locus_tag="ACHATZ_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405158.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACHATZ_002696"
     CDS             4567..4983
                     /locus_tag="ACHATZ_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405159.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACHATZ_002697"
     CDS             5613..>5954
                     /locus_tag="ACHATZ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8405160.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAYYAJ010000154         5878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000154 DAYYAJ010000000
VERSION     DAYYAJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.154"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACHATZ_002698"
     CDS             complement(<1..276)
                     /locus_tag="ACHATZ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405161.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            611..2002
                     /locus_tag="ACHATZ_002699"
     CDS             611..2002
                     /locus_tag="ACHATZ_002699"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8405162.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLRAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FVFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            2061..2864
                     /locus_tag="ACHATZ_002700"
     CDS             2061..2864
                     /locus_tag="ACHATZ_002700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8405163.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2897..3751
                     /locus_tag="ACHATZ_002701"
     CDS             2897..3751
                     /locus_tag="ACHATZ_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFM8405164.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3748..4449
                     /locus_tag="ACHATZ_002702"
     CDS             3748..4449
                     /locus_tag="ACHATZ_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFM8405165.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4457..5524
                     /locus_tag="ACHATZ_002703"
     CDS             4457..5524
                     /locus_tag="ACHATZ_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8405166.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5517..>5878
                     /locus_tag="ACHATZ_002704"
     CDS             5517..>5878
                     /locus_tag="ACHATZ_002704"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8405167.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIP"
BASE COUNT         2022 a          893 c         1133 g         1830 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgttatatga atattgtaac ttgtgaataa ggaattagca ggagaatact
      421 agttcagcat aaaggaagtg atgggacttc tgagacaact ttctgttatg cagaaataca
      481 catataaaca gacaataaac gcttttcccc gtttctttta atttccttta caatttggtt
      541 attcactagg tttttaaact ttgctatggt aagatgatag aggcattatt ttatggttag
      601 gagtgtatat atgaataaaa atggggagtg gaatgcggca cgcagaatat tcattattct
      661 tatggatgta cttgtattaa atgtatcgat ctatcttagt tttttgctga aattccaggg
      721 agaagtacca gtgagaaact tagaaacctt tcagcattca gcaattttta tttccatcat
      781 ctttattgcg ttgaatattt tgttaagagc ttatgtattc tataaccgaa tgatcagtga
      841 tattgtcttc attactgtta ttggacaagt actaatagca ttggggatca tggtagttac
      901 ttttgctggc cgatggtttg tatttccgcg tggcgtcatt ttgatctcgt ttatcctagg
      961 tacgatatta cttagtgttt accggatagt cgtttacaaa ctgtacttaa gagtcagtcg
     1021 tgataagaaa gtccttatcg tgggtttgga gaaagatgtc gcaccagcta tccaaaattt
     1081 tcaatctaag aaaaacaata aacataaggt tgaagcagtg gcaatcgata attattacga
     1141 aaatacaaaa aaattgatcg atgatatgga tatcgtctat ttggcttcta aaatcccaga
     1201 agatgaaaaa atcaacattt atcaattagt gactaaaaaa gagaagaaat tatttttaaa
     1261 tacgactttt gagaatctga caatgataaa tccgaatatt atgaattttg aagatgaaag
     1321 catcattgaa gcttctgggt tcagaatcca accagaatac gaactattca agcgtctctt
     1381 tgatatcatc gtttcgctta ttttactgat cctcgcctca ccttttatgc taatcacagc
     1441 gattttggta aaaacaactt cacctggacc aatcatctat aaacaagtac gtattacgaa
     1501 aaatcaaaag gaattttcaa tctataaatt ccgttccatg agtgcgacag ctgaagcaaa
     1561 atcaggacca gtcttggcta aaagtaacga tgcacgtgtg acaccagtag gtaaatttat
     1621 ccgtgctgta cgttttgacg aattacctca aatctttaat gtattgaaag gcgatatgtc
     1681 gattgtcggt ccaaggccag aaagaccatt tttcgttgat cagttcaatg aagaaaatcc
     1741 ttattattac ttaagacaca atgtacgtgc gggaattacc ggatatgcac aagtatacgg
     1801 aaaatatgta tcagattata acagtaaact acgttttgat ttgttgtata tcaaaaaata
     1861 ttcattacta ttagatttga agattttact tcaaacaatc aaaatcttat ttgataagat
     1921 gtcatctcaa ggactagatg aagatgaaaa tattagttca ttagcagaaa tcgacttttc
     1981 acaagagaaa atttatcgct agattggaaa aattttgagc aaagaggtag gagagaacat
     2041 tggaaaataa aaatgtaagt atgctgcttt ctttatatga taaagaaaag ccagagtatt
     2101 taaaggaagc attaactagt atttttgagc aaacagtaat gccaaaagaa attgtattag
     2161 tttatgacgg ccctataaat gaaaaacttc aagtagttgt cacttatttc caacaaaaag
     2221 atcctcactt gtttacagtt gtaaaattag aaaaaaatca aggattgggg attgctttag
     2281 caattggatt agaacatacg aaaaatcgct tagtagccag aatggataca gatgatataa
     2341 tggaacccca acgattagaa aaacaattag agatcttcca aaaagatcca gaaatagcga
     2401 ttgttgggag taatattgag gaatttactg gcacgcttag caatgttatc ggaaaaagaa
     2461 ttgtacctga atataacaat gcaattcgtg tattttctaa aagaagaaat ccctttaatc
     2521 atatgaccgt tatgtttgat aaaaaagcta ttttggaagt gggaaattac caaccattaa
     2581 gtgggtttga agattattat ctttgggcaa gattattaaa ggcaggttat aaaggatata
     2641 atattcaaga aacattagtc tatgcgcgaa ctggcaaaga tatgtatgca agaagaggcg
     2701 gaatgaaata tcttggttct ggtcttatgg gtcgttatcg tatctggaaa gacggtttag
     2761 ggtcagtaaa agattttata attgtttctt gcggacatat ttttattagc ttactaccaa
     2821 atcaattaag aggaaaattt tatcaaacgc aattaagaaa ataggaggtt tttctttgta
     2881 tagcactgat ccaaaaatga aaaaaataca acagattacg ttgaaaatgt ctcaagagat
     2941 tgttgatttt tgtctaaaaa atcaacttct ttgttatttc tgcggtggtg gtgccatcgg
     3001 tactgtgaga gaacaagggt ttattccatg ggatgatgat ttagatttct ttatgcctag
     3061 agaggattat gaaaaatttt atcaactatg ggttaattca gaaaaaagta aaaaatatcc
     3121 catacagaag gcttctaaaa attataatga tcataattct tttacaacta tccgcgatgc
     3181 agatactaca tttattaaaa cttatcaaag agatttagat atagtccatg gaattactat
     3241 tgatattttc ccgttagatg ttgcccctgt ttcgaaattt agccgtaaaa ttcaaaaact
     3301 atgggcttta gtatatgctt tattttgttc tcaagtcgtc cctgaaaaac atggtggaat
     3361 aatggctaca ggtagtaaga tactcttatc tattttccgc tcacctcgta cgagatataa
     3421 gatatggtct tttgctgaaa aacaaatgac aaaatataat aatgaaccaa cgggctatgt
     3481 gactgaactt tgtgtcggac ctaaatacat gggaaatgtt tatcctgcaa aagactttga
     3541 atctgctgtg tgggtgccat tcgaagatac agagatgcca gtaccagttg gttatgataa
     3601 ttatttaagt caagtttttg gtgactatat gcagcttccg cctgaaaaag atcaggtttc
     3661 tcatcatgaa gcagtttata ttgatccaga acatccatac acaatgtaca aaggaaaata
     3721 ttatttaact aaaggagcag aaaaaaaatg attacagcta ttattattgc tggtggtgtg
     3781 ggtaaacgaa tgggacaaga tatcccaaaa cagttcatta caattgaagg aaaacctatt
     3841 attatctata ctttagaatc atttcaacac catcctcaag ttgaccggat tttagtcgtt
     3901 tgcaaatctg gttgggaaga aacgatgtgg gcgtatatca aagaattcaa tattagcaaa
     3961 gtcaaatggg tcattacagg tggatcaaag ggacaagagt cgatcaataa tggtgttcag
     4021 tttttgaaag actattctga agatgatgat acgatcatca tccatgatgg aatccgtcca
     4081 ctagtagacg aactggtatt gtcagatgta atcgttaaat gtaaagaatt tggtaacggt
     4141 gtaacctctc taccttacaa cgaacaaatt tttatcaaga aaacagaaga aacgacggaa
     4201 cagtatgtcg atcgtaacac attgaggcgt gtctcaactc cacaagctta tcaatacgga
     4261 aaactacggg cagcatatga ccgggcagtg aaagaagaca tcggtatgac tgattcttct
     4321 tatacgaata ccatgatggt agatttggga gaaactttat attttgctgc tggttctgat
     4381 aaaaatatca aattgacaac aacagatgat ctagaattgt tcaaagcata tttaaaaatg
     4441 aaagactagg tgaaagatga tgacgttgat taaacaacca atgtaccaag aagatttggc
     4501 aaaagtaatc aatcatacac caaaaataaa acagctacat ggaaaatccg ttctgatgat
     4561 tggtgcttct ggaatgatcg gctctttttt gattgatacg ttgatggctg ctaatgaaca
     4621 actaggcatc aaaatcaaag tttatgcaat gggcagaaac cgtcagaagc ttgaaaaaag
     4681 gtttgccagt tatcttgaat tagatacttt cgaaattgtt gaaggtgacg tcacagaacc
     4741 cttaccaaaa gaaattcaag ctgactattt gatacacggt gcaagtaaca cacatcctaa
     4801 ggcttacgca actgatccaa tcggtacgat catgactaat cttgcaggta cagagcaagt
     4861 attgaaacat gcagtagcaa cccaaccggt tagagtatta tttttatcaa cagtagaaat
     4921 ctatggcgaa aatcgaggcg atatagaaaa attcacagaa gattactgtg gatacatcga
     4981 ttgtaataca ctgagagcag gctaccctga aggaaaaaga gtcagtgaat cattatgcca
     5041 agcctacatc gcaaaacacg acctcgacat cgtgattcca aggctttgcc gtacgtttgg
     5101 tccgacgatg cttttatctg atacgaaagc atcctcacaa tttattttaa atgcagtcga
     5161 acaaaaagat attgttttaa aaagtgaagg aaaccaatat ttttcgtatg tatatgttgg
     5221 agatgctgtt tctgctattc ttcatttact gcttgaagga gaaaaaggag aggcgtacaa
     5281 cgtttcttca gaaaactttg atttacgact aaaagaatta gcccaacagc tagctgatat
     5341 cagcggcaaa caagtaattt ttgaattgcc ggatgagatt gaacaaaaag gtttctcaaa
     5401 agctagcaca gcagtacttg aaaataaaaa aattaaagag acaggttgga aaccaatttt
     5461 tcctttgaac gaatcattgg ttcatacagt agaattagtt aaaggtgaga aatataatgt
     5521 gtgagattag tattattgtt cctgtgtata aagttgaaaa atatttaaga aaatgtgtag
     5581 attcgatttt agcccaaaca tttactgatt ttgaagttat attagtagat gatggatctc
     5641 ctgacaatag cgggaaaatc tgtgatgagt atgctgaaaa agataatcga gtacgtgtga
     5701 tccataaaga aaacggcgga ttaagcagtg ctagaaatgc ggggattgat gttgcgagag
     5761 gcaaatactt aggctttgtt gacagtgatg attatattga tgaagatatg tatgaaattt
     5821 tgtatgagaa tttgaaaatt catgatgccg acattagctc tgttgaattg attccttt
//
LOCUS       DAYYAJ010000155         5842 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000155 DAYYAJ010000000
VERSION     DAYYAJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.155"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHATZ_002705"
     CDS             196..921
                     /locus_tag="ACHATZ_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405168.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHATZ_002706"
     CDS             911..1420
                     /locus_tag="ACHATZ_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405169.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHATZ_002707"
     CDS             complement(1490..2938)
                     /locus_tag="ACHATZ_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8405170.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHATZ_002708"
     CDS             complement(2938..3654)
                     /locus_tag="ACHATZ_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8405171.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHATZ_002709"
     CDS             complement(3635..3985)
                     /locus_tag="ACHATZ_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8405172.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHATZ_002710"
     CDS             4129..4902
                     /locus_tag="ACHATZ_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8405173.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHATZ_002711"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHATZ_002711"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5744..>5842)
                     /locus_tag="ACHATZ_002712"
     CDS             complement(5744..>5842)
                     /locus_tag="ACHATZ_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8405174.1"
                     /translation="ASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1697 a         1138 c         1014 g         1993 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaatt attatttgtc aaattaagcg agacaaaact
     5761 ttccatttac gtaactcaaa aaaacttcta atggtgtttg gtaatttagt gattttctag
     5821 ggatatggtt tcttttggac gc
//
LOCUS       DAYYAJ010000156         5665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000156 DAYYAJ010000000
VERSION     DAYYAJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.157"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(249..752)
                     /locus_tag="ACHATZ_002713"
     CDS             complement(249..752)
                     /locus_tag="ACHATZ_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8405175.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1111..2136
                     /locus_tag="ACHATZ_002714"
     CDS             1111..2136
                     /locus_tag="ACHATZ_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8405176.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2459..3337
                     /locus_tag="ACHATZ_002715"
                     /pseudo
     CDS             2459..3337
                     /locus_tag="ACHATZ_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            3813..3929
                     /locus_tag="ACHATZ_002716"
     CDS             3813..3929
                     /locus_tag="ACHATZ_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8405177.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4857..5195
                     /locus_tag="ACHATZ_002717"
     CDS             4857..5195
                     /locus_tag="ACHATZ_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405178.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5289..5519
                     /locus_tag="ACHATZ_002718"
     CDS             5289..5519
                     /locus_tag="ACHATZ_002718"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8405179.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1906 a          851 c         1044 g         1864 t
ORIGIN      
        1 aattcctttc tagattgaga tattgactag gaagaatgtt aagattttaa gcctttaagc
       61 taaaaaaaaa ccctttttgt taaataaggg gaatatagcc tgtcagtttc aaactgcaag
      121 tcgtcctgct gcaataatat agtagattag agatgaaaat agattttttt aaaccaatga
      181 ctgaataaaa agaccgtcat agatacacat tggaatgtgt ctattacggt ctttttttac
      241 gaacgatttt attgaagacg gacagttcgg gtcaaaaata aaaaagctac gatcagaaat
      301 aagaataaaa tgtatcccaa aataatggct agacggtcta acttatttgt cgatagatgt
      361 ttgtttagaa gttgataggc ccataaacct atcagtcctc ctgttgtatt agttatcaaa
      421 tcagtgatgt ccgctacgcc aataccgaat aggaattgaa tcaattctgc caagaagctt
      481 acaaggaaaa taatccctaa ttttttagaa aaagaaagtc tcttgaaatt gacattcaaa
      541 agcaccccaa aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa taccgtcgta
      601 ttttcaaaag aaaaagtaga aaaagggacg agattgacat tgctgtaaga ataagtgaga
      661 aattgtggaa gattaaatga tagcttgaat aaaatcaacc ataatagaaa gaataaataa
      721 atccctaata aaaattttga tacataccgc atagacatct cctccattta aaaagtatac
      781 catcgaaaac cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa cgtagttatc
      841 gtacgaaaat tagaagcttt tttttgttgg gaatccggtt tctttaacaa agctaagata
      901 gcgagttgct tggaaaagtt gctatctttt ttctgtcaaa acgactcgga atgttcgttt
      961 tttgttctaa tttctccaag ttgtttactt tttgatacta agtttatgaa aaatcaagaa
     1021 atagttgtta tactaggtaa tgctgacaaa cgggattggc atttcatctt ggttcagctt
     1081 tttttccaat tactaggaag gagggataga ttgttcaaga cttattttcg tttcaataaa
     1141 atacaagata atccttttcg tatcatgaat ctgatgctac ttatctttat tgtattatgg
     1201 ttaggttaca taaatcatga ttttttgatt tcaagttttt catcattagg gatctttact
     1261 tttttctatt atcaaaacat accgatgaaa tatttgctga aacgtatgtt tctgatcggt
     1321 tgcggactgt tggttgcttt cgttttgggg atcttatcga cttatgtttt atggcttgaa
     1381 ccatttgccg tagctttggt tgctttttcc agcagattcg tgttgcgatt attccatatt
     1441 tctaaaccag gtggcttgtt ttttgcgatg ctttctgcca tgggaacgag catgcagctg
     1501 ccaatcgctc aattaccgat cgtctcactt tattttttta tgggtgtcgt ttttgccctg
     1561 attgcggcag tgattacaaa attactggat tcacggcctg agcagacaat tgaaaaagca
     1621 actttaaaag aaagatttca cgaagagcct ttagtgatca ttgattctgt tttctacagc
     1681 gctgccttat ttctttccgt ttatgtcagt cacggcttga atctgcataa tccttattgg
     1741 ttgaccttgt catgtgcatc gattttgcta gcagaaaact tggatgcgat gaagcatcga
     1801 caagtccaat atctgattgg atcgatgggt gggttatgtg tgtcggcatt cttgtcattt
     1861 gtgccgttta cgcagttgca gaccattttt ctgatcactt ttctatatgg aatcgcacaa
     1921 tttctagttg ctagaaatta tgcagttgct aatatttttc ttaatccgat ggcgttgatg
     1981 ctatctacac ttattcgtgg agcatacttg atttctttga tcgaatatcg ttttttaggg
     2041 atcgtcatag gaagtttcat cggtttaggt gttgcgtggg taatgacggt tggtttgcaa
     2101 cattatttaa cggttgttcg tcaaaaatta gaatagaagg acaaatatat gtgccaaaag
     2161 aggttatgac aagatttttg tcacaacctc taattcaagg tgttcaaaag ccagattctc
     2221 agcattaagt caaattcaca caaaaatgtg gaaaggaact ttcaaaaaaa ttttatattt
     2281 attctatcaa aaaattttgt ttatgatggt aactgaataa actttcctga gatggtcaat
     2341 gctttccttg aaagtcttac agatattctt tcttcataga aagtttaagt tttatagtta
     2401 gtgtacaata aaagtatgtt tataccatat ggtgtccgga taatcagtca tttaaacaat
     2461 gaggttcaaa gatatgaaaa ggggaaattt gatgaagaaa ataagcaatg aaatccaaat
     2521 tctggcagaa actttaactc ttcccaaagt cgttatagag caaatgattg tacaagcaaa
     2581 aaacatcctt gatgaggtgt ttgagcaata tagtcatgaa ctaatggatc ctaataaagc
     2641 aaaggcgact gcaaagaaaa tagacaaagt ctatgaagag gaaccttttg cagcattagc
     2701 tatttatcta tatgcagctt ctcaaagttg gaaaaaaatt tatcaaccat tgaagattcc
     2761 aagaactatc tatttggcaa caatgaatgc ttttactaga tttattcatg aacatttcca
     2821 agtaacagga agctatcgct ttgaccgtgg tttttggatt tggcattaca ctagtggctt
     2881 gatctttcgg attggtgaat tggagtacga aatgacatat tttccgaaag ggtacacgga
     2941 tctaagatta gaaggaaagc agtgcctttc tcttcatatt ccttccgatg cagatttatc
     3001 tcagaataag ataagagata gttacgaaag tgccattcgt ttttttgaac tttattttcc
     3061 tgattaccat taccaagtga tgtacacaga tacttggtta ctatcaccca atcttactaa
     3121 atagttgaaa aaagagtcaa aaatctgtct ttttgcagcc gattatcgat tattatcagt
     3181 tgatgaacag gacgatagtg gagtcccttg gatctttgga cgagtagatg cccaaataca
     3241 tgattatcca gaaagtacga gtttacagcg acaagcaaaa gagcaattgc ttgcaggcga
     3301 gcacattggc tctggcttag gtattcttac ttcataagtt tagtacttcg aaatagttta
     3361 aaagagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
     3421 gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
     3481 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctttttta
     3541 gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa
     3601 agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc
     3661 cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt
     3721 aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg
     3781 actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat
     3841 accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag
     3901 atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg
     3961 aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg
     4021 ctgttgattt tccattttta acatatgata aattggtaga atctttcttt tcaaattgat
     4081 accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata
     4141 aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta
     4201 ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg
     4261 aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag
     4321 cgagcaatac ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat
     4381 atctctaacg ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc
     4441 tttctatttg tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat
     4501 aaaaaagcag ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat
     4561 aatagtcgat aagttgtgta ttggaatttt agatacagta ataaagtatt tttcatgaaa
     4621 ttttgtgaga atcactatta caagtatctt taataaaatt aatggttaag gaggaactta
     4681 aagtagaatg taaatgaata gttgtagtca gtcgattgtg atactagact caaagttgca
     4741 ggaataagtt tttcaatttg tgattggaag ataaaaaatg aaaaagaagg gctttttttt
     4801 atactttgtt gattgtagtg tttattttcc ctctgtttag ccaagtaaca ttttctatga
     4861 ctcaaccagt ttcaagtgag gaacatgata acctttttcg tgctacagaa gagtttaaga
     4921 aatatcagga aaatattgtt ccggagactc cattagctag tgagccaatt actgatattg
     4981 atggaaacca aataggatgg tatatacagt acgaagttat ggtggtaaat aatgactcaa
     5041 tagtaaaaaa tacagtaggc ttagagctaa caccgattga aaaggtgagt tcattgttaa
     5101 cgtatattta tactgatgaa agtgacgaga ctgagatcat tttaacggat tactcaaaaa
     5161 ttgctactga taaaaagtat atattgtgga tgtagacact agtgaaatac tttatgatat
     5221 agaaattgat gaaaatttac tagatgtagc acaagaaatc gatcatcaaa agcaggaagc
     5281 tattcgatat gctggtagaa aagggaaaag aaaatattta ttacgtgaat gtagcaaaag
     5341 ttagagaaga tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt aactcgactc
     5401 cgacatttac agtttacaga gaaggatcga tagaaaaaac tgttttttgg actaaagaaa
     5461 gtggaatgtc tttagctgaa gtagaagagt ttttggatta tgttagtatg caacagtaag
     5521 ggagttctta ttatgaaaaa ttttgacaca tattatgtta ttgttttttc gatatctttg
     5581 ctgttaatat tactaacttt aatgcagtat aaagagtatc cagagtattt tattttaact
     5641 tttgtatatt tagtaagtag ctaca
//
LOCUS       DAYYAJ010000157         5544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000157 DAYYAJ010000000
VERSION     DAYYAJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.159"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACHATZ_002719"
     CDS             complement(56..1561)
                     /locus_tag="ACHATZ_002719"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8405180.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACHATZ_002720"
     CDS             complement(1653..2243)
                     /locus_tag="ACHATZ_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8405181.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACHATZ_002721"
     CDS             2422..4068
                     /locus_tag="ACHATZ_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8405182.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACHATZ_002722"
     CDS             4117..4836
                     /locus_tag="ACHATZ_002722"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8405183.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..>5544
                     /locus_tag="ACHATZ_002723"
     CDS             5315..>5544
                     /locus_tag="ACHATZ_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8405184.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSS"
BASE COUNT         1668 a         1008 c         1122 g         1746 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttc
//
LOCUS       DAYYAJ010000158         5541 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000158 DAYYAJ010000000
VERSION     DAYYAJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.160"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..1604)
                     /locus_tag="ACHATZ_002724"
     CDS             complement(144..1604)
                     /locus_tag="ACHATZ_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405185.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(1812..2738)
                     /gene="glsA"
                     /locus_tag="ACHATZ_002725"
     CDS             complement(1812..2738)
                     /gene="glsA"
                     /locus_tag="ACHATZ_002725"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8405186.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2761..4188)
                     /locus_tag="ACHATZ_002726"
     CDS             complement(2761..4188)
                     /locus_tag="ACHATZ_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8405187.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(4219..5433)
                     /locus_tag="ACHATZ_002727"
     CDS             complement(4219..5433)
                     /locus_tag="ACHATZ_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8405188.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1743 a         1147 c          936 g         1715 t
ORIGIN      
        1 ttgactctaa gtcaatagca ttttaaaaaa atttcagaga aagcttgtta tataagtaga
       61 tcttcgcacg aatcaggact caaataaaaa ccttctacag acaattaaaa agctttgtag
      121 aaggtctcct aattaaggtc tcatcaagaa atatgttttt ttaataggtg aagttcctga
      181 gcaatatgat acaaatggat gtttgcacct tttaatgaga ttggatagat ctttgtcttt
      241 tcagtttcta atccattttg atagctagca gcatcgcaca aaaccactct ttttttcgca
      301 catagacatt catcaggcaa ttcttctaat gaacgaaata aaataggatt tatttcagcg
      361 atgccttctt ccgcatacaa atgaaattgg gttcgataaa aaaattgttt gactgaactt
      421 tctgaaacaa caaaaatttc tatttcctgt ctccccgctt gttcactgtt gatcaaagaa
      481 agacttttga cgaattttcg aatgaaattt tcattccatc ttagttggat ttgatttttc
      541 tttttccact cttccaatat ttctacaatt tttccgtaca gaagttgttc tttttctgat
      601 agaagataga tcctttccgg taaaaatact tgtccatctg cccacaacgt acggacaaat
      661 tgtaaaaagg attgcttgaa aataaaattt tctgcatcgt cgatttcagc gatactaata
      721 atctgtaaaa tcaattcatt cagtgatagg tcattgagaa caaaattttt ataaacaact
      781 tcgaaatctt ttcttaacag ctccatgttc tcattggtat aatttctact gttgaacaaa
      841 gaaaaaatat aatagacatc ttcttcttgt aagtctagaa ttcctttttg ctcttccaat
      901 ccttgacaga ctaattgaaa taacggcttc gtttctgcgc ttttcttatc ttcttctttc
      961 agtccaaggg gaaatgcctt tcttccgatt gcgatctcta ttcctcgtat gataaaggac
     1021 ttttcatctt ccgaatatct tcttttaaaa aaatgagtct ctacataatc aatcaactcg
     1081 ttacaagctt cttctacata cggattttta ttttcatagc cttcccaatt gacataacgt
     1141 actaaagcta gcaagatgtt ccgcgagtca aattctggga taatgatttc tttatttttc
     1201 aaataatcat tttctttaaa aaaatccaat acgatttttt tgattgtata tactttgctc
     1261 aaagaaataa attcaagatc agccagttct gtactcgtga atataccatt aaaataataa
     1321 cttaaaaact ttaaaaaata agattgaccg tacagaaact gtgtgagctc agaaagagca
     1381 ataccatgtt tgaatacgag tttatactta tgcttctctc ttacttgttc ttttatacaa
     1441 tattctagac actcggctag actttctaga tcgtgaagga tcgttggtgt agtcaccctt
     1501 agagaaatag ctgtttgttc gatgtctatt tcttggaatt caaataattg ttcacacaga
     1561 tacacttttc taaatatatt tcgctcgatg taattttcca gcattcctga tacacgcctc
     1621 cagtttttct tatgatttta tttaatcata tctgtaaatc gtatttttgt ttccttttta
     1681 tttagaaaag ccctttcaaa aaccaataaa aaaagaaact gggacaaaag tcaaacaagc
     1741 tcacaatttc agcctcattg taggtagctt tgagccgttt ggacttttac cccagcctct
     1801 ttcaagaatg gttattcaaa gatgtcagcg tctagttctt tgactacatc tttcaacaat
     1861 tggataccag ctgcgctgtt accaaatgga tctagtgccg ggctgaagac acctatgccg
     1921 taacgatttg gcacagctgc catcaacccg ccgcctacac cgctttttgc aggtacacca
     1981 acatggacgg aaaactctcc tgattcatca tacaaaccag cagttgtcat aattgatttg
     2041 acgattgtag cactttcttc agtaatgatt tgtttgccat tccaaggagc aattccttta
     2101 tttgccagca cagcagctaa ttttgctatg cctgtagcgg tgacattgag ggagcattgt
     2161 ctgaaataag catcgagtat ctccggtacg tctccttcga tcatttgatt acctttcata
     2221 tagtaagcaa gagagcgatt gatatcacct gttcttgatt cagaatgata gatttcttca
     2281 ttcaatgtga tattaggatc atcacagatc tctttcataa atgacaagat tcgctcaaat
     2341 ttttcatctg catctttacc ggcaatcaac gaggttgttg caatcgctcc tgcattaaca
     2401 aaaggattca ttggatgttt gcgatgattg atttccatat tcatgataga attgaaggca
     2461 aaaccggttg gttccgtgtt gattgtttga aagacttttt ctattccgtt gtctttgatt
     2521 gctaataaca atacgggaac ttttgacatg ctttcgatgg caaagcgcac atcggcgtct
     2581 cctccctcaa tcagttgttt tgtatccaaa tcataaatcg ccatacctaa ttgtttggga
     2641 tccacttcag ctaatgctgg aatgtaatcc gccacttgtc ctgtttgata atagtggaaa
     2701 ttttttccaa tcaattgttc taattctttt acttccattt gaataaccag ctttcatttt
     2761 ttatttttct gcatgctgga atttatgacg gcatgcgtag atcgtaaatg gaataactaa
     2821 gacgataaga taagaaacaa tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat
     2881 actagcaggt ggaacaaatg agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca
     2941 agcaacgatt acttttacaa cttttccgcc tggaacctga taggctcttt caagactgtc
     3001 atctttaaaa accagcttca aatagcccgc gaaaaacata aagtacaaca tcaggtagat
     3061 tacaacagtc aatgacatcg ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt
     3121 aaagacggct gcccagattg ttgcaacgat cccatttgcg atcatcaaat gagcaggaac
     3181 accgtgtgtg ttttcttttg caaaatattc aggaagtaat ccttcttctg ctgctacgtg
     3241 aagtgctttt gaaggagagg ttacccaaga agcaatttct ccgatgacac ctaaacaaac
     3301 aagaatcgca agaattttcg caatccatgc aagatttgga tcaatgtgtc ttaataggaa
     3361 ttctaacgat tgaatcaccc ctgtattaag agacaaacca tgttgaggta ctgtagtagc
     3421 gacggtcaat ccgccaaaag tatctaaaat gattgccaga ataaccaata caaacattgc
     3481 taatggataa tcctttttag ggttttccat ttcatttgca tgactggcag atgtttctac
     3541 gcccatatat gctaaaataa aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc
     3601 aggaatcaat gttttcatag acagtatcgt ttgtagtgga gcgcctgaag cgacataatg
     3661 gatccctaag ccaaacaaga ctaatgctgg caacaagata ccagctacaa aaccgatccg
     3721 agcaatcaat gaagtgtatt tggttccttt taactgtgag agtaaaatag cccagaaaat
     3781 aatcaatagg attgccaatt tcagatattt attgttttgt atagcaccaa tacccgttgc
     3841 ataggataat gcaccagtta agaaatacag catggtcaag tagccaactg ttatctcaaa
     3901 ccattggaaa aagattgcgg caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt
     3961 atagacaccg cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac
     4021 agggataaac catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt
     4081 cgcgaatgtg ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc
     4141 aaagaacgtt aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct
     4201 tttctatttg aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc
     4261 ttcttgttct tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac
     4321 tgttgtcagc acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa
     4381 gaagtaccag ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag
     4441 aatcgtacct gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca
     4501 ttgtttcttt ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat
     4561 cagcatggca ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc
     4621 aacggatcct gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat
     4681 cagccaagta atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc
     4741 ttgttcatct gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag
     4801 tcctgtaccg atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat
     4861 ctttctttga ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat
     4921 gactgctcca ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat
     4981 ccagtggcaa acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc
     5041 ttttaaattc aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa
     5101 catcagttgg taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg
     5161 gaagcttgta acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat
     5221 atcccgtcca aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac
     5281 ggagagaaaa gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta
     5341 aaaaaaagct agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc
     5401 tgtatctcct gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg
     5461 agtataaaag aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt
     5521 ataaaagaca gacaattaat a
//
LOCUS       DAYYAJ010000159         5349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000159 DAYYAJ010000000
VERSION     DAYYAJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.161"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..474
                     /locus_tag="ACHATZ_002728"
     CDS             <1..474
                     /locus_tag="ACHATZ_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8405189.1"
                     /translation="KELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNW
                     KLQQKEEYLKLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFR
                     HTHASLLLEAGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(1102..1587)
                     /locus_tag="ACHATZ_002729"
     CDS             complement(1102..1587)
                     /locus_tag="ACHATZ_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8405190.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(1589..2260)
                     /locus_tag="ACHATZ_002730"
     CDS             complement(1589..2260)
                     /locus_tag="ACHATZ_002730"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405191.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(2523..3716)
                     /locus_tag="ACHATZ_002731"
     CDS             complement(2523..3716)
                     /locus_tag="ACHATZ_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8405192.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3800..4000)
                     /locus_tag="ACHATZ_002732"
     CDS             complement(3800..4000)
                     /locus_tag="ACHATZ_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8405193.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(4424..4657)
                     /locus_tag="ACHATZ_002733"
     CDS             complement(4424..4657)
                     /locus_tag="ACHATZ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405194.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4664..5095)
                     /locus_tag="ACHATZ_002734"
     CDS             complement(4664..5095)
                     /locus_tag="ACHATZ_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8405195.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1452 a         1231 c         1004 g         1662 t
ORIGIN      
        1 aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa
       61 tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac tctttctatc
      121 ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc
      181 tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt
      241 aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg
      301 tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa
      361 caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat
      421 aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc
      481 tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat
      541 atcaagggtt ctagctccta taacaggttt acccaacact gccttccaat gactcttttg
      601 ggaatcagag gaaagaatca ggctttatag agatatttca tctaacaaat tactttagac
      661 tgacagtata tatcagcagg tactgtcatc gggaacgtag ccaatatcgt atccaaaaca
      721 gcaggtagag aagctatttg aggattcaag ttattctatt tcaaccatga agttaagttc
      781 attaaaaata aagatagttg gtatttcaat aatttttgta tagagttaat cactttattg
      841 atgctaattc atcttgattg gtattataat cgtattcaag ttgcgtggcg tgcttttgct
      901 agaaaatata taactgttct ttataaagaa atataaggat taaacatttt ttatcataga
      961 gttttactta tctatagaat tatgtctgtt cctaattcag tttgatgaat taactgataa
     1021 aaaaatatct actatttaaa gatacaggga taagtaagta aaaaaataac atgtcattag
     1081 atagtagaca gttccaactc tttatttacc ttttttgtat gctgcactgt caatccaagt
     1141 tcctaaattg ttgtatccat catcacttat ataatcatat tggccatcaa taatttcgtc
     1201 aaaccacaaa tattctccat ctggtttttg cccaattatt ttaattactg attgactagt
     1261 agttgtcatc tggttaaaat cttttatttt acctatatta acggccaaaa tagcatttcc
     1321 tcgcttataa ctctcctgga tttcatattt aacatattct cgatcaagag tttctttacc
     1381 tatcagcacc acagtcactg atgttccaat taattgatta tctatccagt tttttatagc
     1441 aatatctcca ttacgtttaa ctttctcaaa ttcagcctta tctacaaaac cagcttcctt
     1501 tgttcctcgc aaagtccagc tgtttctaac taccattgct cttgttacat ctgcataatg
     1561 gaaactaaaa aaaacccttc tcaccatatt aatcctcctg aatactattt attaaactga
     1621 caatttgttc aactttttta tctacttctc cttcagtatc taatttttct agcgcttgaa
     1681 atttactttt taaggaatca tcaaaataat cactgaaatt ctcttgaatc ttatcaaaca
     1741 aaaccttact tgcacctttg gtaaatccaa tcggaactac tatattttca ttttgaacag
     1801 ataattcaaa ttcttcaatc attccatctg ctgtaactat ttcacctgat tcaagtttgt
     1861 ttccaaaaac aaaaatacta atcccagact ctgcaataat gttttctcga tatcttctcc
     1921 actcatgagg catatcaagg ggaagtggtc ttaaagaaag tacgtcttgt atgttatttt
     1981 ttcttaaatt tcttctctgc tttagaactc ctgaaacaat actattacca attccaagtc
     2041 cgtaacctga tactattcta taaccggatt cagaaagcct atatgctaat ttctcgacaa
     2101 attttaatcc tttcgattca gatgtaaatg gagtataatc aacagcactt cccgaaataa
     2161 aaatagtttt tctcaaatac ttttttctaa tacttttcaa aatatcagta atctcagaat
     2221 aatcatctac ttcaacaacg tttatcgaaa atctggacaa atcattgatt tgtagtcttc
     2281 tttttgtcag ttcataatta tatctctctt gatcaaaaaa atcctcagaa tcgacaaaat
     2341 tttcctttaa aattatatgg gattttagat tatcttttta gaagttcttg aatgtcggta
     2401 tagcccgtgt ttccgggctt ttccggcatt tttgatattg ttagatactt acaaatgttc
     2461 ttattacctc tcaccatttc cccctcaaaa gtagtaaatg gagtagtaaa accggccttg
     2521 tgtcaggctg ccagccgttc catttctgcc cttgcggaat cgaatgtggc gtgtgcataa
     2581 tagttcagcg tcatggtgat attggaatgt cccataatgt actgcaaggc tttggggttc
     2641 attcccgcat tggctaaccg ggtgcagaat gtatgacgca tagcatgggg cgtcgtcgtt
     2701 ttcggcaacg cttcttcatg gctcttgttg tacttcttca caagcctgcc aaacatcccg
     2761 ccataatcta ccgccgtcat gggatagccg ttctgtttga gaaacaggaa attggcatac
     2821 ccgtcaataa tgaacggctt tgcacctttg cggtttttta tcactcgctg gaacgcctga
     2881 tacacttcct cattcatggg gatttttctg acgccgcttt gggtcttggg ttcgtcaata
     2941 tagtaaccga cgccggtatt tctcaaaagc tggtggctca cgatgattac ccggttttca
     3001 aaatccaaat ctttgtccgt cagtccgcac agttccgaaa tgcgaagccc tgtccccagc
     3061 agaataacga ttgcgtcata atgcttttgg taaaccttat caccttgaat aaaggacaga
     3121 aaactttctt cctgtgctgg tgtaagaggc acctttggtt ccgtatcgtc gtccagaacg
     3181 tcactaagct ggaagtcaaa cgggtttttt cggatgcaat cgtcctgtat ggcggtgtaa
     3241 aaagccgctt tcagggaacg cttgtcgtta ttgatggtct tataagaaag gcccttttct
     3301 ttcatccgaa gcgcccattc ctttgcgtcg gacagcttca cactgtcaat cgggcagctc
     3361 cccagccgat cttgttccag aatacgcatc agccgttccc ggcccagttt ggtgttgtgc
     3421 ttcacattgc cccggtgcct gatctgcttg gcgtagagct ggcagacggt cattttcttg
     3481 ccgatggtgt ctatcccgtc gtcaaggtct ttctggattt ctttctcctt ttcccggagt
     3541 gaaatatcct cccgtttacc tgccggggtc ttatccgtag gcactaactt ccatgagtaa
     3601 acaaactgcg gttttccaaa ggtatcaaca tatttgtaag catatcgtcc gtcttttctc
     3661 tggctctctc cagaccgcaa aacgcgattt ttgctgtctc gtcttttttc tgacatctcg
     3721 tcatgctcct ttccatgacg gaaagagccg catatatgct tataaattat tataacatat
     3781 ctgcggccct ttttcactat tagattgtgt ccaaagtgtc aattattttc tcaaactgct
     3841 tgcgtttgat ctggatgcgg ttgccattca taatgaccca gccagaggcc ggattttcct
     3901 cggccagctt acgcaatttg ttttctccga tacggaaata tttagacgct tcctcaatcg
     3961 tcagcgtgta tttttcccaa ataggcacat catggttcat aatgcagccc cctttcacaa
     4021 attcaaaggt ttgtgtgatt taggggcata gacggacggc tgctggcaca gctccacggg
     4081 actttcaccc ccgcgtggtt ctcacgcggc cctacccatt gcctgcgacg cttttaacgc
     4141 tcggactgtg gctataaggg agtatcatag tcctgcctgt acgtcgtcgc ccgcaagccg
     4201 ccacgctcac attgcggcgt ggtgttcctc gctccgcttt tcccgtaggg aaaagtatca
     4261 tggcgcacca gccgtccggc tcggcacaga cagaatgtgt ccgtatgccc tatgatgcgc
     4321 cgccgttatc ttgcgggcat atttatcaag gtgcgatcag gccaccggga aaacaccgaa
     4381 acggggaaag ggtaagccgt ttcatgctgc cggggccggt tcgtcagttc gtgacgatag
     4441 cgtggatgag cttgatttcc agacggcgct tcatggcctc gtccagacac acatgcggat
     4501 agccgttttc gtcatagagc gtccgggtac acagcttgtt catgtaaccg ctgtaatact
     4561 gcaaaacgcg ctctacggct ccggcatccc cagcgcgggc ggcctcgatc accgaaaggg
     4621 gcaaaagctc aatgcccttg tagttcgggt tcttcatccg cgtctaacct cccttcggca
     4681 tcaacgcctt tagctgtttc cgcagctccg ataaggtatt tgtcctgtgc cgctgaacgg
     4741 cactccggga cattcccaca aggccgccga tctcaccatc tgccatatcc agaacacagt
     4801 gcaggattaa aatactctgc tccatgctgg gcagcgcggc aaaggcttcg gcaacaagct
     4861 cattgttgat atgtaggtca tagccatgcg acgaaaacgt aaagctgtcg ctggggtagt
     4921 gatctactgt acagagcttg tccaaatctg cctgtgacaa agagcttagt gacacttcac
     4981 ggtcacggcg gcgcttcatt tcagccaagt agcttttggc ctcgttacgc agtaccgtct
     5041 tgcaaaaagc atcgaacatg caccgcgctt catagtcgcg ggggatagca tccatcttct
     5101 cacccccttt ctgtggggga cttaggtggt tctctccctt tccgctaaca agactttgac
     5161 aaggccgcca gctacccgct aaatacctcc tttttgaaaa tttctcaaaa aatttttttg
     5221 gggacagccg ggccgcaaaa gacgacaaaa atcgcccggc aggtcgcaaa cctaccgggc
     5281 gagaaaatgc tttgctatgc tgctgattta catggtatag tacatactca taaccggaat
     5341 agccctgtg
//
LOCUS       DAYYAJ010000160         5312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000160 DAYYAJ010000000
VERSION     DAYYAJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.162"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHATZ_002735"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHATZ_002735"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8405196.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHATZ_002736"
     CDS             complement(1297..2133)
                     /locus_tag="ACHATZ_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405197.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHATZ_002737"
     CDS             complement(2317..3534)
                     /locus_tag="ACHATZ_002737"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8405198.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHATZ_002738"
     CDS             complement(3683..3844)
                     /locus_tag="ACHATZ_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405199.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHATZ_002739"
     tRNA            3917..4000
                     /locus_tag="ACHATZ_002739"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACHATZ_002740"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACHATZ_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAYYAJ010000161         5285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000161 DAYYAJ010000000
VERSION     DAYYAJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.163"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACHATZ_002741"
     CDS             <1..546
                     /locus_tag="ACHATZ_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8405200.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACHATZ_002742"
     CDS             complement(716..1678)
                     /locus_tag="ACHATZ_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8405201.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACHATZ_002743"
     CDS             complement(1680..3119)
                     /locus_tag="ACHATZ_002743"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8405202.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACHATZ_002744"
     CDS             3304..5250
                     /locus_tag="ACHATZ_002744"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8405203.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1693 a          909 c          886 g         1797 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaggca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttc
//
LOCUS       DAYYAJ010000162         5254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000162 DAYYAJ010000000
VERSION     DAYYAJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.164"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..361
                     /locus_tag="ACHATZ_002745"
     CDS             5..361
                     /locus_tag="ACHATZ_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM8405204.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            377..727
                     /locus_tag="ACHATZ_002746"
     CDS             377..727
                     /locus_tag="ACHATZ_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM8405205.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            756..1535
                     /locus_tag="ACHATZ_002747"
     CDS             756..1535
                     /locus_tag="ACHATZ_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM8405206.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1546..2223
                     /locus_tag="ACHATZ_002748"
     CDS             1546..2223
                     /locus_tag="ACHATZ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM8405207.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2242..3348
                     /locus_tag="ACHATZ_002749"
     CDS             2242..3348
                     /locus_tag="ACHATZ_002749"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM8405208.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3338..4429
                     /locus_tag="ACHATZ_002750"
     CDS             3338..4429
                     /locus_tag="ACHATZ_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFM8405209.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4442..5182
                     /locus_tag="ACHATZ_002751"
     CDS             4442..5182
                     /locus_tag="ACHATZ_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM8405210.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1683 a          863 c         1253 g         1455 t
ORIGIN      
        1 aatcatgatt aaagtagtta ttgcagaccg tatgggtaaa ggacaaaatg tggccaaagg
       61 ggtagaagca gctggtggaa aagcagttgt cgtaccagga atgggggcag atatgcgttt
      121 gggcgatgtg atgcaacaag agcatgcaga tatgggtatc tctttttgtg gtagtggtgg
      181 tgcaggtgca ttaacagcag caacaaaata tggctatcca gaacgacatg gtatgcgttc
      241 gattgaagaa gggatcacag ctatcaagga tggcaaaact gttctaggtt tcggctttat
      301 ggatcaagag gaattaggga aacgtctgac agaagaattt atcaaaaagc atggaaaata
      361 gaaagaggga catgaaatga gcgttcaaaa gacaattcag caaaaagtta tcgtctccgg
      421 taaaggggaa acaaagcaac atgcttttgc atcagcgtta gcagaaattc aaaacaaaat
      481 aatggaacaa tcaaacgtat tattgcgaat cgaaccagtc aatgtggata ttttaaaggc
      541 ggtcgaaaac aaatacacag aacgctttct atttttcttc ttcccacggc aacgtattag
      601 ctatgaagtc gaactctctg ttactgtggc tgtaacagaa ataaaattgg cagacatatc
      661 gttcagccag caaatgactg aagatccaaa tgggattgcc attccatttg taactaaaaa
      721 aatctaattt tgaatcataa gggggaaaga ataacgtgga tttcgtaatc attctagtca
      781 aatcggtcat tatcggggga ttacttggtt ttgcagcatc tgtcggggca gctcgaatgt
      841 ttcatgcacc taatacacaa ggtttaggag cttttagaac attaggcgaa atgaatgcct
      901 gcatgggtga tccagcttct catttttcat ttggtttagg ctttttcttt aatgcatggg
      961 cttcttcagt tggtgctggt gcattcacac aagatgtgac gcatcgaatc attcccaatt
     1021 gggcagcggc agcacttcta gcaaaaaaca aagatgtcac ccagaccatg catgatccga
     1081 aaaaaatggg gatcattgga gcggttatcg gggcagtggt ggtagccttc ttaaacacaa
     1141 cagctgcagc aattccagag gcgttacaag tgacggcggt ttccgttttg gtaccagcag
     1201 cgacgattct gatcaataca gtaatgccag ttattttttg gttggcagct ttagacgcag
     1261 ggaaacgaac tggtttttgg ggcactcttt ttggtggtat cgcgcaattg attatgggaa
     1321 atgcggtacc tggtgttgtt ctaggaatct tgattggtaa aggcgtcgat gagagcggtt
     1381 ggaatcgcgt cacgaagagc atgatgatcg ctattgtttt attatttatt ttcagtggat
     1441 ttttccgtgg atttgatatg gaaatgatca agagcttcca gcttcaagtg cctaattggc
     1501 ttgataactt gcacaacatc tttagtgtga aataggagga gaatcatgga gacaacagaa
     1561 gaacaagcaa aagtattaga agcaaagcgg caaaagagct tttggtttgc agactggtct
     1621 tttccgctaa tcgtaggaat tatgtccgct gctgtatttg caggaacaca catgtatgtg
     1681 atgtatggtg tgggtgcatt caatgaagtt tcgatcgtgg cgatgttaaa agccgggctg
     1741 gatggcggat cctatggcgc agcggcagcg tttggtgcta gtttcttatt tgccagaatt
     1801 ttagaaggct ctttagttgg gatcttggac ttaggcggtt cgatcctaac tgggatcgga
     1861 attggggtac cagcaattct cttaagtatg aaaatagcgg caccaatcga taattttgca
     1921 ctctctttat tgactggttt ggttctaggt ttgattgttg gtggcatcat tgtcttgatt
     1981 cgtaaattta cgatcaatca atcaaattcc acttttggtg ctgacgtcat gatgggggca
     2041 ggaaattcat ctggacgttt tttgggtcca ttaattatat taagcgcgtg tgcagcatct
     2101 attccaatcg gcattggcgc tacattagga gcactaggat tctatgcttg gaaaaaacca
     2161 attgctggag gtgctatttt aggagcaatg attttaggag caatcttccc ggcagtaaat
     2221 taatagaaag agggtcatta aatgtttgat actttgatta aaaatggaag gttagttgat
     2281 ggatctaaaa tagaggtcgc tatagaaaaa ggcatgatta aagcagttgc agcagaaata
     2341 aatcagccgg cgcaagaagt agttgacttg gagggaaagt attatttatc cgctgggtgg
     2401 atcgatgatc atgtccactg ctatgaaaag atgaatcttt attatgacta tcctgatcag
     2461 ataggggtcg aaaaaggtgt gacgacggtc attgatgctg gaactacggg agcagaaaac
     2521 atccgtgaat tttatcaatt gacgaaatcg gtcaagacaa atgtgtatgc gctaatgaat
     2581 atctctaaat ggggcattgt caaacaagat gaacttgctg atctaacaaa gattcaagaa
     2641 gatctagtaa gtactgcgtt agcagaactg ccggaattta ttgtcgggat taaagcgaga
     2701 atgagtaaga cagttgttgg tgaaaatggc attaagccat tagaattagc taaaaaaatc
     2761 cagacagaaa acaatgatct gccattgatg gttcatatcg gttctgcacc tcctgagtta
     2821 gaagaagtat tgtcaagaat ggaaagaggc gatgtattga cccattgttt taatggaaaa
     2881 ccaaacggta ttttagatcc aaagacagat caaattaaaa actttgtctg ggatgcttac
     2941 aataatggtg tcgtctttga tattggacat gggacggata gctttaattt ccatgtggca
     3001 gaaaccgcac taaaagcagg aatgaaagct acctctatta gtacggatat ctacatccgc
     3061 aatcgtacaa atggcccagt ttttgatcta gcaactactt tagaaaaatt gagggtagtc
     3121 ggctattctt gggaagaaat cattgaaaaa gttacagcag ttcctgcacg gaattttcat
     3181 ttgaagaata agggccagtt agctgtcaat tatgatgcag atttgacgat atttgagatt
     3241 gttgaaaatg aaaaggaatt gaaagattcc aacggattta cacgtatagc taaagaacaa
     3301 attttaccga ttaaaacaat tattggaggc gtcatatatg acaattagct atggaaagtt
     3361 tgatttaaaa gaagtcatta atgcatcggg aaaaatgacg atcctagggg tttctaaggt
     3421 atctgaaagt gtcctagcag cacaacgatt tggaggcgag catttttttg agatgagtga
     3481 tcttgccatc aaaaccggcg cttatctttc tcaattactg aaagtggaag atgcgcaaat
     3541 cgtttcttct gcttcagcag ggattgctca atctgttgct gctttaatcg gacagggcga
     3601 catgtaccat gtctatcatc cctatacaga aaaaatcgct agacgggaaa tcatcatacc
     3661 taagggacat aatgtagatt atgggacacc ggttgaagta atggttgctc aaggtggagg
     3721 aaaagtcata gaagctggct atgcaaatat gtgtacaccg gaacatgttt ccatgatgat
     3781 tacggaaaaa actgctgcgc ttctatatat taaaagtcat catgcagttc agaaaagtat
     3841 gctgacggtg gcagaaatgg tagaagttgc caaagctcat cgtttgccat tgatcgttga
     3901 tgcagctgcg gaagaagatt tattcaaata tagcgaaatg ggcgttgact tagtgattta
     3961 ctctggggca aaagcaatcg aaggtccgag tgccggactg gtcataggaa aaaagaagta
     4021 tattcaatgg attcggctac agtctaaagg tattggccga gcaatgaaaa tcggtaaaga
     4081 taatatttta ggctttaccc aggcactaga agattattta aaaaatggga gcgaatccgg
     4141 tgactccatg aagcaacgat tagcgccttt tgttaacgca atcaatcaac ttcctgacct
     4201 ttcagcgaaa atagttcaag atggtgccgg cagagatatt tatcgagcaa gtgtaacgat
     4261 taatggcgat aaaagtgcga aagaagtcat tcaggaatta aaagcacgaa atccagcagt
     4321 ctacacccga gaatatcaag caaataacgg aatcattgag ttcgacattc gttccgtcag
     4381 ccaaaacgaa atggaaaaaa ttataaaaag attgagagaa atcatgtagg aggcttttaa
     4441 attgtcagtt atcccaaatt attataaaaa tcgtgtatgt ttaaatgtat tagctcattc
     4501 agttgaaaat gcaaaagcat gctatgaagc agcggaaggg catgtcgtac taggtgtgct
     4561 atcgaaaaat tatgctagcg atgaagcagc aatcgaagat atgaaaaggt accaagacgc
     4621 aaccaataat gcgttgtcag ttggattagg cgctggagat ccaaaccaaa gtcagatggt
     4681 ttcgcgttta tctggggttt tacaaccaca acatgtcaat caagtattta ctggcgtggg
     4741 gacttctcgt gctttattgg gccaaaatga tacagtcatc aatggattgg tttcaccaac
     4801 aggtaagatc ggctatgtca atattgctac aggtccgtta agttcacaag cgccagcagc
     4861 tgaagtgcct attgaaactg ccattcaaat gctgcaagac atgggaggta cttcaatcaa
     4921 gtatttcccg atgaaaggct tagcacatag ggaagaattt aaagcagttg cggaagcttg
     4981 cgcaaaatat gaattctatt tagagccaac aggaggaatt gatctttcaa actttgaaga
     5041 aattctgaaa attgcaatcg atgcaggtgt aaaaaaagtg attcctcatg tttatagttc
     5101 aattatagat aaagaaacag gagatacgcg tataggagac gtaaagatac tattaagcat
     5161 ggtaaaaaaa attattcact aactcattag aaaataagaa gcttattcca gaatgaaatt
     5221 aaatggaata agctttttat aattaattac ggaa
//
LOCUS       DAYYAJ010000163         5237 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000163 DAYYAJ010000000
VERSION     DAYYAJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.165"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1042)
                     /locus_tag="ACHATZ_002752"
     CDS             complement(38..1042)
                     /locus_tag="ACHATZ_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8405211.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1113..1409)
                     /locus_tag="ACHATZ_002753"
     CDS             complement(1113..1409)
                     /locus_tag="ACHATZ_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405212.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1402..1695)
                     /locus_tag="ACHATZ_002754"
     CDS             complement(1402..1695)
                     /locus_tag="ACHATZ_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8405213.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1841..2392)
                     /locus_tag="ACHATZ_002755"
     CDS             complement(1841..2392)
                     /locus_tag="ACHATZ_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8405214.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2566..2865)
                     /locus_tag="ACHATZ_002756"
     CDS             complement(2566..2865)
                     /locus_tag="ACHATZ_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8405215.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2892..3200)
                     /locus_tag="ACHATZ_002757"
     CDS             complement(2892..3200)
                     /locus_tag="ACHATZ_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8405216.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3317..4276)
                     /locus_tag="ACHATZ_002758"
     CDS             complement(3317..4276)
                     /locus_tag="ACHATZ_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8405217.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4420..5196
                     /locus_tag="ACHATZ_002759"
     CDS             4420..5196
                     /locus_tag="ACHATZ_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8405218.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1565 a          966 c          814 g         1892 t
ORIGIN      
        1 tcaaaatcag tttttctttg ataaataata ttttcaatta tttaatcatt tgaaaattgt
       61 tagtcattcc aatacgttga gaaattcttc catctgtata aacacggaat tgttttccac
      121 cagccattgt tgtatttcca ttatcatccg ttacaatatt aattgaatag tagcttccat
      181 tttcatcttg cattagatct ttcatatatt catttgtgct aattaaatta gtatttgatg
      241 catttttatc aggagaatca tacaaccata aagctttatt atttagtaca gtttctttag
      301 cagcttccca acttgaaatt gctgagctac ttgtagattg tgtcgttgta gttgttgctt
      361 gttgagtaga ttcttctact actggagttt gaggagtttg tccattcaca atatcaatga
      421 aaccttgttt taactgttgg ttctgagttt cattgaagta catgtagttt ttatcattgc
      481 cttgattttg ttgcgttaaa taaactttag gttgtccatt ttgaaagcca aagaaatata
      541 catgctgccc taaatattct ccagtttcta catcagaata aactgctact aaattgaaat
      601 ctgcatgacc agttccatct tctgaccatt gtacggtaac tggtgcttca ttgattgcca
      661 ttttccaatt tccatcaata actgcacttg gtaaaggcgt accatacaga cttacattgt
      721 tctgaggatt gtagctttta tattcttgac ctaatgtctt tccccattgt gggacaaaaa
      781 ttgctagttg agtagatttc tcactatccc acaaagtatt tttaacctct tcagaagaag
      841 attcaatgat tgaactttca cttgtacttg cttccacaga ttgtttcatt tctgttgtag
      901 tttgttcagt tgattgcgtt ttactttttt tagcctcaat tgttgtatca gtttctacag
      961 tctctttagt cctcgtattc tcagatgtac aaccagtgaa taaaatcccc attccaatta
     1021 gtaataacaa ccattttttc attttatttc cccctttcaa tttctccctc gcgtacttat
     1081 tttatcagtc aattaatctc aatggtgttc atttagttaa tcctttccca agaatgtcga
     1141 tagccagcat gacaacgatc tcttccttga gggctttcag atggattaaa actagtagaa
     1201 atttcccttc cacatcctct acagcgataa gttctcatta tttttgggct attagtatta
     1261 tttttcttag ttagagctat gagtccaact atgattaccc caactgtctt aattactttt
     1321 cctttatcta tattcggatg cgcaagcata tatttatata accaatatcc accgtaaatt
     1381 aaaccaccta atacaatcag cttagccatg tactcgtctc ctttcttttt gtttaattac
     1441 tttttgaatc catcttgcag agaaaccata aacttctgtc aattgacgga tatctacagt
     1501 tttttcctgt tctatcatgt tttcgattct ttttctaact aactcacgat cgtacatttt
     1561 cattggaaaa gaaatctgtt gccctcgaaa agcaagataa aattgataga gcaattctga
     1621 atccagttcc atttcctcaa aaaagtcatt catactttta taagagttac tcaaacaagt
     1681 ttcatcaatc tccatctaca caccccctgt tattttttac ataatgaaaa tagcatatta
     1741 ctgggtacat tgataattca tataacatgt atcatcacat gtatttttaa agtcaaaaag
     1801 catgtaaaac tacttataag taacataaga aaataaaatg ttatttttct tgaaataata
     1861 attgactatc tgccaattgc cattcattac gaacatgaat acttagtggg tgggataact
     1921 taccttctgc atttatcaat actttggtca tctttttttg tttcgcactc aattgatcgt
     1981 aaatggtctt cgcttgttca taagccactt gactcgtcgc tgtaatcgaa ccacaagcaa
     2041 aaataatggt gttgattttc ttttctgtga aaacagttgt taatatttcc aaagattgtt
     2101 catcatttcc acgaggaaat acccgaggac ttttcgcttt tgtatacgaa aaaagattca
     2161 cagcaataaa accagtaaat cctaattgtc ggatattctt ttcgattaat aacatactca
     2221 aatcattcgt aaatggttct gtatctgttg gacgaatagt aatgactacc gctaatttat
     2281 cttccgcttc ttttcctttg atcgcccatt gtcgtttgaa aacatagcga tgggtatgat
     2341 gaggatctcg ccaaacttct gtttgtgtgg tcactaattc tttttcaatc atctcttttt
     2401 ctcctatgct aatttttgtt caagcaattc aatcaccatt gccgttataa gtgtcaatac
     2461 tagttgaaaa tatttcatat tctttctcct tttttcttca gaatttgatg aatcgctgta
     2521 aggatatctt ctaaacattt cgcaatcatt ctcaaataat cgatcttatt cattcgttcc
     2581 tccaatctga ctccaaacca atcgtccttc taccagtaac ccacgaacaa attctcgctg
     2641 gctggtaata tctgtaatga aataaactac atagtctttt ccatcttctg gtgcgcaata
     2701 aatataaaga tgtgtcatca ctaaacgtgg tagagtaaca tcttcaggaa gcatacccat
     2761 ttcaatttcc tctttttcca tcgtaacgga ttcatagccc cactcttcag gagtagtgac
     2821 tagctgtgca cacttttcca gtacttcgat agccgtttct aacaagtcct ttcgttcctt
     2881 tcttttagat atcagtattc ttctggtaat aacatcgttt gatactcacc cgaatcaatg
     2941 acccatattt tttgagacac tgatttcaaa cgaagcttag gtgaaatcag atactctttt
     3001 cttgcttctg gttcttcttg acgatgaacc aacttttgat ttcctttttc cgtagaaaat
     3061 tcaaagattt gaagatagtc taaatgaaat tgcttttgaa cgttttgatc gattagattc
     3121 caacataaag cttgtaattc caccggcaaa ctgtccatta ctccctttgt gacataacgt
     3181 tgtcctttat tttgaaacat ttcttttccc tcctttgcca taaaaatagc ccaatcttct
     3241 attaaagaag attgggcttc atggatataa tatatatttg atattcaatt tgattcattt
     3301 tatactatct gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa
     3361 atacttccaa tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg
     3421 caacggattg aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat
     3481 ctcgtctaag caagccgttt gagtgctcat ttagccctct ttgcgaaggt gttcctggat
     3541 ctgcgaaaaa gatggcaata tcgttcgagt tacttataga tcgccagttg gaaaattctt
     3601 ttccacaatc aaatgtcatg gatttaaaca agtgtttagg catggatttg aaccaatcat
     3661 ttaacctgtt ttcgatatct tcggctcgtc tgcctgtcgg tttcaaagta ataataacct
     3721 tggataaaca ctcaaccaat gtaataacag cgctcttgtg tttaactccc acaatggtgt
     3781 cgacttcgag atgaccaaat tctttagtca ggtgtggata gagactgttc cgttcatgaa
     3841 tcgttctgcg aaaggcctgt tttccacgct tttcttgatg attattcggt ttacgtttcc
     3901 ccttcatagg taacgactgt gtgtcaaaaa ggcctttttt aaacatccga tagagtgtac
     3961 gaacagaaca atcaattgga aactcgccac ggccgataat cacatcgggt gaccatcctt
     4021 gagctacctt ttcttgaaca tatgttgatt gttcatcaga taaaacggtt gaacgtcgtc
     4081 cacaacgaga tttattttgt ttgtattgac ggtaataatc tagcactttt tttccttgtt
     4141 tgagaaacga ataaacatta taaatggttt gtctagagcg atttaaactt tgtgcgacaa
     4201 gagcgacagg tttctcgata ttgaaataag actctatgat tactagttcg tctgttgtaa
     4261 gatgggtgta agtcatttgt actcactcct tattaattct ttggtcggaa ctattttgag
     4321 tgtagcacaa atgatctttt tagttgtcta gcttaatttt acaatcgacg ttttaataaa
     4381 ttgtgatata atgaagtata actttttagg gagtgtacta tgaattttca agataataga
     4441 ccattatatt tacaagtaga agcttctata cgtaacgata ttcgtgaaaa aaaatatctt
     4501 ccaggagagc aattacctac agaagaagaa ttatgtaaaa tttatggagt aagtaagatt
     4561 accattcgaa aagcttttaa acttttagca gaaagtggaa cagttgaacg attaaggggg
     4621 aaagggacct ttgtaactca aaaaaaagaa agtttaaaat tagctggagt tcatggattt
     4681 agcgattctc tttctacccg aggacataaa attagatata ctgttttaca ttctaaaatt
     4741 ataaaggctg atgagttttt agctaataaa ttacaaatca atgtaactga tagagtttac
     4801 aatattaaac gtttaatgtg ggaagacggt gccccgatgg gaattgataa ttttttcgca
     4861 tcagaagaaa aatttccaac tttatttgaa aaaggtagcc aatatacttc attatataaa
     4921 cttctagaag atgactatga tgtcaaagct acgagctcta ttatggaaat aaacggttta
     4981 tctgcaacac ctgagctttc cgacttattg caatgtatag taggagatcc gctatttatt
     5041 ttagaaaagg ttggcaaaga taaaaataat caaccatttc attattctca atccacagtt
     5101 cgttgtgata gaattagtta tgttattgaa gtcacagaaa atatcacttt agatacggtg
     5161 cataaaaatg attcagatcc taaagtaata gattgatatt aaaagaaatc atacccaaag
     5221 caatcaattt tgattag
//
LOCUS       DAYYAJ010000164         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000164 DAYYAJ010000000
VERSION     DAYYAJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.166"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHATZ_002760"
     CDS             85..972
                     /locus_tag="ACHATZ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8405219.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHATZ_002761"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHATZ_002761"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8405220.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHATZ_002762"
     CDS             2274..2720
                     /locus_tag="ACHATZ_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8405221.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHATZ_002763"
     CDS             2993..4345
                     /locus_tag="ACHATZ_002763"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8405222.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHATZ_002764"
     CDS             4516..4803
                     /locus_tag="ACHATZ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8405223.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYAJ010000165         4928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000165 DAYYAJ010000000
VERSION     DAYYAJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.167"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..851
                     /locus_tag="ACHATZ_002765"
     CDS             39..851
                     /locus_tag="ACHATZ_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8405224.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            848..1648
                     /locus_tag="ACHATZ_002766"
     CDS             848..1648
                     /locus_tag="ACHATZ_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8405225.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1809..3077
                     /gene="rseP"
                     /locus_tag="ACHATZ_002767"
     CDS             1809..3077
                     /gene="rseP"
                     /locus_tag="ACHATZ_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8405226.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3145..4854
                     /locus_tag="ACHATZ_002768"
     CDS             3145..4854
                     /locus_tag="ACHATZ_002768"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8405227.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
BASE COUNT         1603 a          817 c         1105 g         1403 t
ORIGIN      
        1 aagatagtgg gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa
       61 aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc
      121 agcacattgc catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac
      181 gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa
      241 ggcttggagt gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa
      301 aagatgaagt aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag
      361 aattgattaa agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac
      421 atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat
      481 tgaattttgc tttgaattat ggaagtagag cagagatcgt gactgctgtc caagaaattg
      541 ccgaagaagt agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta
      601 agcatttaat gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta
      661 caagtggaga agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt
      721 attttacaaa agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct
      781 cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg
      841 atcagtcatg aaacaacgtg tgatcacagc agctgtggcg ctagtcttgt ttattccgat
      901 tatatggatc ggaggcatag cggttgagct tacagcagca atcttagcgg tagtaggagt
      961 ttacgagctg ttccggatga aaggattgac actcttaagt tttgaaggag ttttatccgc
     1021 cgttggtgca gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa
     1081 tgtaagtaca tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat
     1141 ttctaaaaat acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt
     1201 gggaataggt ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt
     1261 tggtctattt attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg
     1321 tcagcataag ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg
     1381 tatcggtagt gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact
     1441 attcaatcat ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca
     1501 atttggtgat ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa
     1561 tatcttacca ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc
     1621 tatcatgcat ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat
     1681 aaaagacgca ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat
     1741 ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga
     1801 aaaaagctat gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc
     1861 atgagtttgg tcatttcttc tttgcgaaac gatcaggaat cctcgtccgc gaatttgcta
     1921 tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc
     1981 gcctattacc tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa
     2041 tggcaccagg aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa
     2101 atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt
     2161 tagaagatga gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct
     2221 accctgttga tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc
     2281 caaaagacgt acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg
     2341 gacccatgaa taactttatt ttggcaatcg tgttattcat cattctggca tttatgcaag
     2401 gtggtgttca agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg
     2461 cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt
     2521 catggaatga tctgacaaca gtcattacga aaaatccagg taaaacatta gactttaaga
     2581 tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg
     2641 gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga
     2701 tcattggagg gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat
     2761 cactatttac aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt
     2821 cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa
     2881 tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct
     2941 tgaatatttt tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga
     3001 cattagctgg ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc
     3061 aacgattctt tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt
     3121 tgaggaaata aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc
     3181 gaagttccaa atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc
     3241 cgccaagtat cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa
     3301 ctgaaacgca ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct
     3361 gccatcttgc cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac
     3421 ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa
     3481 acttttactg atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt
     3541 tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga
     3601 agagagttta tcatgaaaga tgcatattct ttccatgcaa cggaagaaag tttggacgag
     3661 acatataaag attacgaaaa agcctatacc gaagtattca agcgttgtgg cttagaattt
     3721 cgctcgatca tcggagacgg cggcgcaatg ggcgggaaag actcaaagga attcatggcg
     3781 atctcagaaa tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac
     3841 ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg
     3901 gagaaagtag ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt
     3961 gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt
     4021 ttggtacgtg gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac
     4081 tttttagaag aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct
     4141 gtcggaccag tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat
     4201 ttagcgaatg ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca
     4261 aaacgcgatt tcactgtgct ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct
     4321 tcaccagaca acaacggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatattc
     4381 aaaatcggca ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga
     4441 gaaaaatttg tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc
     4501 gtagaacaac attcagatga acaaggaatc aattggccaa aaggaattgc gccatttgat
     4561 ctgcatatcg tccaaatgaa tctaaaagac gaataccaaa caagcctgac gaatgaacta
     4621 gaagaagcga tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca
     4681 ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa
     4741 aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta
     4801 cgtaaagaag aattggcgaa tactttacca atcctcttga accaaggcga ataataagcg
     4861 ttaaaacaca gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct
     4921 tttaaaat
//
LOCUS       DAYYAJ010000166         4891 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000166 DAYYAJ010000000
VERSION     DAYYAJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.168"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..1727)
                     /locus_tag="ACHATZ_002769"
     CDS             complement(117..1727)
                     /locus_tag="ACHATZ_002769"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8405228.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1949..2194)
                     /locus_tag="ACHATZ_002770"
     CDS             complement(1949..2194)
                     /locus_tag="ACHATZ_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405229.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFGTHKGLKK"
     gene            complement(2920..4260)
                     /gene="glnA"
                     /locus_tag="ACHATZ_002771"
     CDS             complement(2920..4260)
                     /gene="glnA"
                     /locus_tag="ACHATZ_002771"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8405230.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEVKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4288..4680)
                     /locus_tag="ACHATZ_002772"
     CDS             complement(4288..4680)
                     /locus_tag="ACHATZ_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8405231.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1439 a         1053 c          797 g         1602 t
ORIGIN      
        1 ttttgctttc tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca
       61 agaaaattgc ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat
      121 tttgtttcgt gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga
      181 tttggacgag aaatcaattc tgggtggaat tggcatccca caaagaattt tttctctggc
      241 agctctacga tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt
      301 tcttcaaaca attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc
      361 acaacatcgg ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg
      421 tataatccaa ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa
      481 tcaataatat tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca
      541 acgacgttac gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac
      601 ggaacgtcat tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca
      661 ccaaatccac caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct
      721 ctcgttaatt cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca
      781 gaatgtttca atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg
      841 acaagcgcaa tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat
      901 tctgtcatgt ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt
      961 ccttgtgctt gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata
     1021 actgcttctg gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca
     1081 ggttgctctg tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt
     1141 acactgtgct gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat
     1201 gttgtatgga tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct
     1261 tcaaggaatg gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca
     1321 tctgaatcag tcattttagc tgctcgcatg attttttctt tgatctcatt tgtaatatga
     1381 ggaataactt gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca
     1441 gaatagattt ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt
     1501 tcgtagtggc ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg
     1561 tgctgatatg ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata
     1621 gttactttta agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt
     1681 ccgattgatg aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact
     1741 cctttttaat ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa
     1801 taagaatagg gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg
     1861 tataatacaa gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat
     1921 tttttctttt tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgccaaaaa
     1981 aatttaaccc acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca
     2041 gaaaatcgtt actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta
     2101 tgatagataa tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat
     2161 atagcttatc gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa
     2221 acgtagtcca cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca
     2281 aaacaattcc actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg
     2341 attcattgaa ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc
     2401 ctccttattt ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
     2461 ccatcaaaca tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag
     2521 gctcacaacc tctggataaa cggtattcaa aagccagttt ctcggtatta agccaaattt
     2581 atgcaaaaat gtagaaagcc attttcacaa aatttttctt aatactcgaa actaaacgtc
     2641 tttttcaaaa cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagttcag
     2701 ccaattcaaa aacaagaaaa gctgtttttg cttggctatt cttattactt gttgcagggc
     2761 agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc
     2821 aaaaaacggc aaaatatgag gaactatttt tccgttagtt tctctaatac tcaggtcaaa
     2881 acgctgattt cacagcctct aattcacggt attcctgttt taataaagtt ctagatattg
     2941 ttctctttcc cactcggaaa cagtttgtct gaatgccgcc cattcgagac gttttacttc
     3001 aacaaaattt gagaagatat gttgtcctaa tgcgtcaatc attacattat ccttacgcaa
     3061 ttctttgata gcattatgga tagttgaagg taagtcctgg atatgtgcag cttctctttc
     3121 ttcctcatcc attacataaa tgttacggtc aacagctgct ggcggtgtga tctgattacg
     3181 gataccgtct agtcctgctt gcaacaatac tgccattgcc aagtatgggt ttgcagatgg
     3241 atcaactgac cgcaattcca aacgtgtgga taaaccgcga gattctggta cacgtactaa
     3301 tggcgaacga ttacggccac tccatgcgac ataaacaggc gcttcataac ctggaacgag
     3361 acgtttataa gaattgactg ttggattgca aacggctgtg taggcacgag catgtttcag
     3421 caaaccacct aagaaatgat aagctgtttc acttagttcc aattcaccgt ctttatcata
     3481 gaaaacattt ccttcatcat tgaacaaaga catattgcaa tgcataccag aaccattgat
     3541 cccaaataac ggtttcggca tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt
     3601 gacaacgagc ttgaaggttt ggatattatc acaagcatct accacatctg catatttaaa
     3661 gtcaatttca tgctgtcctg gcgctacttc atgatgtgaa gcttccactt caaagcctag
     3721 actttctaat tccaacacga tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa
     3781 atcaaaataa ccacctttat cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt
     3841 aaataagaag aattctggtt caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat
     3901 atctttcaat gctcgtttca aattcccgcg aggatcgccg gcaaatggtg tcccatccgg
     3961 attatagata tcacaaatta aacgagcaac ttttccatga gcactttccc acgggaagat
     4021 catccaagtg gagagatctg gatacaaata catatcgctt tcttcaattc gaacaaatcc
     4081 ttcaatcgaa gaaccgtcaa acatcatttt attttctaat actttgtcga tctggctcac
     4141 tggaacttct acatttttga tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg
     4201 tacgttttct tcctcaatga ttcgtttgat ttcttcacct gttatctgtt tctttaccat
     4261 ggtatccctc ttcctttttt aatttattca gtcaaagaat tgtctttgca atccttgcga
     4321 ctgataatga cctgttttgc ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg
     4381 tcgaacgtca tgatctgtca acggcttctt gttttttgct tcttgttttt tcgcctgtgc
     4441 catttcgtat acacgtttga tcccagccat attcaatccc tctgaaagat agtctttaat
     4501 ctcaagcaac atatcaatat cattcagcga atacatacgt cggtttcctt cgcttcgttc
     4561 cggatgaact aaatcttgtt cttcataata gcgaatttgc cgagcagaca aatcagtcag
     4621 cttcataact gtaccaattg gaaatacgga catagatcgg cgcaattcct tttctcccat
     4681 cagacgtccc ctttcgtttt ataagttggc taaagtgtac caattaaaaa aaatattgtc
     4741 aactgcatat gttaggtttt ctaacatgag atctctattt tttataaaat aacaaaaaaa
     4801 taagaacaca aaaaaggtat ctagttgatt ttcgtaccag atacctttta ttttttatta
     4861 tactgtacta tcctttatta gtgtttttat t
//
LOCUS       DAYYAJ010000167         4774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000167 DAYYAJ010000000
VERSION     DAYYAJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.169"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHATZ_002773"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHATZ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8405232.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACHATZ_002774"
     CDS             complement(2064..3062)
                     /locus_tag="ACHATZ_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8405233.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACHATZ_002775"
     CDS             complement(3288..3941)
                     /locus_tag="ACHATZ_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8405234.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHATZ_002776"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHATZ_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8405235.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1402 a         1004 c          744 g         1624 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gcta
//
LOCUS       DAYYAJ010000168         4622 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000168 DAYYAJ010000000
VERSION     DAYYAJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.170"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..2106
                     /locus_tag="ACHATZ_002777"
     CDS             166..2106
                     /locus_tag="ACHATZ_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8405236.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2193..4115
                     /locus_tag="ACHATZ_002778"
     CDS             2193..4115
                     /locus_tag="ACHATZ_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8405237.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4395..>4622)
                     /locus_tag="ACHATZ_002779"
     CDS             complement(4395..>4622)
                     /locus_tag="ACHATZ_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFM8405238.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1532 a          781 c          942 g         1367 t
ORIGIN      
        1 tgtaagattt tccaatatta ttttattcaa cataataaat cgtatatcaa tttttttagc
       61 gaaatttcta tttattccaa gaatttcagt tattacggaa tcattttgtg cttaataaaa
      121 agaaaccgtt tttatatact ataaatgtag aaaggcggta ggaaaatgaa aaaaattatt
      181 gcaatcacat cctgtccagt tggtattgcc catacataca tggcagcaga aaacttagag
      241 aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa cacatggttc catcggtatt
      301 gaaaatgaat tgactgcaag agatatcgaa gaagcagctg gtgtaatcat cgcagcagat
      361 actaaaatag ataaatcacg attcggcggt aaacctttga taaccgtagg tgtgcaagaa
      421 ggaatccacc atgcagatga gttggtgaga gatattttag caggaaaagc gccagtctat
      481 aaaagtacgg aggcaattat ttcttccgaa aataaaagtg aatcagaaaa tttaggtaaa
      541 aaaatctata agagtttgat gaatggcgtt tcgtacatgg tacctttcgt agttacagga
      601 gggttattga tcgcgatttc tttaacatta ggaggaacag ctacacctga agggatcaag
      661 ataccagaag gaacgatttg ggcaacgatg aatagtatcg gaagtatcgc tatggggttg
      721 atggttccaa ttctatctgc atttattgcg caaagtattg cagatcgtcc aggacttgtt
      781 cctggatttg ttggtgggat gttagcggca aacggagcat tatatggtag tgatgcgaat
      841 gcgggattct taggcgggat catcactgga tttttagcag gattcattgc tttaggaatc
      901 aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc caattattgt tatcccaatt
      961 ttaggatcat tggcagttgg ttttgtgttt atctatgtta ttggagaacc agtagctctt
     1021 ctatttagtt cattgactca ctttttagca gggatgcaag gaggaagtga aattgttcta
     1081 gctatgatct taggagcgat gattgctttt gatatggggg ggccgttcaa taaagtagcc
     1141 tttttgttcg gatctggctt gattgcagaa ggcaattatt ccattatggg gccaattgct
     1201 gtagctatct gtatccctcc attagcattg gggctggcat cattgatttt aaaaaccaaa
     1261 tttacgaaaa cagaacgaga agctggtaag gcgtccttag caatgggact gtttgggatt
     1321 acagaaggcg ctattccgtt tgcttcaaca gatccactac gggtgattcc aagcattgta
     1381 atcggggcaa tgacaggaag tgtgatcgcg atgctttcag gagtgacaga tcatgtagct
     1441 catggcggtc cgatcgtagc ggttttagga gcagtggatc acgtagccat gttttttgta
     1501 gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa gaatccttaa accgacatta
     1561 caacaaactg cggttgctgc agcaaatggt gtgacagtag cagatctatc agagactgaa
     1621 acaagtgatg tttatacgaa aactgaaact tttgattcta tcacggatat tatgtcagat
     1681 gaagcaatcg ttttaggtga aacagcaaca acaaaagacg gagtaatcga agatttagtc
     1741 caacgtttag aaaaaacagc ggcgatcaca gataaaacca gatttaaaga agtcatctat
     1801 gatagagaac atgaaagtac aacgggaatc ggtatgggga ttgctattcc acatggaaaa
     1861 tcagcttatg ttcttaaacc aacagtggca tttgccagaa gtcaatctgg ggtagattgg
     1921 cattctttag atggaaaacc tgcacatatg atctttatga ttgctgtacc tgaaaacagc
     1981 caaggtgata tgcacttgaa aatactgcaa cgtctatcac gtaaattgat ggatgatgac
     2041 ttccgtcaag cactgatgga agcgccagat aaaacaacag ttcaccaatt attaagcgaa
     2101 atgtaacata agatgaaaaa tcgaccgtga tgagacataa tagatgtgtt atcatgatcg
     2161 attttttatt tagaagggag tttctgcaca ttatgaatca gcgtcagcaa tctttgatct
     2221 atcaattaag tgagaacaat aattatataa caacaaaaaa gctagcagag catttccatg
     2281 tgtcagaaag aacgattcat agcgatttga ataccattga aacttggttg agagacaaac
     2341 atctgccttt aatgtttgaa agaaaaaaag gtacaggtat cttgttgaat ggtctgcctc
     2401 acgaaaggga acaactgaaa cgagcgctta atgaagagac tcaaacagaa atggataaaa
     2461 aacagcttcg tcagttattg attttccatc tgctagtagc aaaagacgga tttagtcttg
     2521 aagaactttc agaaaaacta tatgttggaa aacgtacgat tcgtaaagaa ttgacagagt
     2581 tgcaaagctt ttttgagtat caccatttac agctagtatc caaaacaaaa ctaggaactt
     2641 ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt taagacatta cgaaatatgc
     2701 agaaagaaga tccacaaagt ccgtcgttga aagaattctt cgctaaagat acattaaaag
     2761 ttatccaaca tacattacaa gatgtttttt atgaaaacca tttagaacag ccgaatggac
     2821 tggctagtgt ggagattcac atctacttta tgctcgagcg aatgaaacag taccagaaag
     2881 tgaaattaag taaagacgaa aacgaagtcg tggaacacac ccaagcacaa aagttaagta
     2941 gtcaaatatt agcaaaacta gccaccatct atccaattga attttctcct gatgaaatca
     3001 attatctggc tttgcgtatc gccaatcttt ttacaaattc acaagcagcc acacgttttc
     3061 aaaaggaatc tagcgcactt gctgatcatt tgatcgaaca agtggaacag tttctaggct
     3121 attcattaaa agaagatcag ctactaaaac aaaatttaag gtctcattta tcttcaactt
     3181 attttcgact ccattatggt ttgcagattt ctaatccatt gacgaaaaat gtgtttagta
     3241 cgtacacaca acttttctta gttttacagt taattttaga agattatttt gaaaaggaaa
     3301 atttttatgt tccacaagag gagacagctt atttaacgat ccatttccaa gcagcgatcg
     3361 agcgacacaa acatcagaaa agtagaagat atcaaacggt tttagttagt gaatattcaa
     3421 aagctatggc tacttttctc gaagcacggc tgaacagaga acttccagaa ttaacgatta
     3481 tggatctagt tgaatataag ccagaattaa agagacaaga ttttccctct gttgatttta
     3541 tcttggctac gttaccattt aaacatataa gcattccagt aatcgagatc tcaccaatga
     3601 tcacagaaac tgatctagcc tatttgacaa aatatatgct ggagcatgtg cctatcaaga
     3661 agaaaaaaac atttgatcta gcaagcttta ctcatccatt tctgatcttt cctcaacttg
     3721 aacggacaga cccagccgat attttaaact ttatgggaaa cgtcttagtg gaacatcatt
     3781 atgtggagcc ggaattcatc gattctgtct tggaaagaga cagtcactcc agtactagag
     3841 tagcaccatt tgtaacaatc cctcacggta atccgctgta tgtgaagcat tcgatgattt
     3901 ctattgctac aatgaaagaa ccagtcctat ggcacgggga acagatacgt atcgtactgc
     3961 tattagcaat aaagaaaaat gatttgaaac atgcagagtt caaaaaaata ttttctgtta
     4021 ttcactatct agaaaagcag ccagaacaaa tggctcagtt gatgcaatcc aatcatccgc
     4081 tagaaatctt aacgttgtta tctagttatg aataagtagc gattaaaaaa ctgcctttat
     4141 atttataata attctatcta tttatttgga agtagaaaac taaatacaaa aaaagaggat
     4201 gtggccaaag ttgtggcaca tccttttatt ttgagtttct tataattttt tatagttgct
     4261 tacgatgtct tgactactgg taaacggtgt tcaagagaca atttgtcggc aataaggcac
     4321 aattttgtaa aatatggaaa gctatttttt taacattgtt tcttagtgct tgaaaaatca
     4381 ttttgaatac ttctttatac gatcgatgta cgttgattta agtctaaaac ttttcttttg
     4441 attccttctc tgacttttct gaattccgtt aatatttctt cttcgctccc gacagcttgt
     4501 gcaggatctt ttaggtccca gtgaaggtgt tcgtgattgt ttggaacggc agggcacttg
     4561 tctttggcat caccacaaag tgtaatgatc aagtctgcct gagtaaagta atctctatca
     4621 at
//
LOCUS       DAYYAJ010000169         4613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000169 DAYYAJ010000000
VERSION     DAYYAJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.171"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..1144
                     /locus_tag="ACHATZ_002780"
     CDS             206..1144
                     /locus_tag="ACHATZ_002780"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM8405239.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1396..2406
                     /locus_tag="ACHATZ_002781"
     CDS             1396..2406
                     /locus_tag="ACHATZ_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFM8405240.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2774..4198
                     /locus_tag="ACHATZ_002782"
     CDS             2774..4198
                     /locus_tag="ACHATZ_002782"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8405241.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            4284..>4613
                     /locus_tag="ACHATZ_002783"
     CDS             4284..>4613
                     /locus_tag="ACHATZ_002783"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405242.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCK"
BASE COUNT         1585 a          589 c          838 g         1601 t
ORIGIN      
        1 ttataagaga acaaaagtaa agaattatat ctacttgctt gcgtatttct tttttatcat
       61 tgtgtgtttg atattcatca atataagagg aactgctttt gtaaatggaa tcctaccatt
      121 atttagagaa aatattattt ttaaggctgt ttactatttt tttgagtaag aataaatagc
      181 acttaaaaat agagatgagg gatttatggg aagacaagaa ttatatagtt tagctatcaa
      241 tgaattggaa aaaagaaatc ttgaaataca tgaattaaat caaaaaaata attctatttt
      301 aggcatgaaa atacgaaata aaattaatca acttaaccaa atgaaaaaac gagataatct
      361 gttgccagca aaatataccc ttaaatcgat gaccggtaag gggcctatta aagactattc
      421 aaacagagag aatcgcagtg atgaactaag aaaaaaagag ttaactaatc aaggaacacc
      481 taaaaggatt gctgtgtata gttgtatcac tggtaattat gattctatta aagaaccgga
      541 tttttctcat ccattggtag attactattt gtatacagat aatgtgatta caagtgagca
      601 ttggaatatt agagagatac ctaaatatat atcggaatta aaaaataata ttttgatcaa
      661 tagatatatt aaatttcatc ctaatgagct atttactgac tacgatttta ctttgtatgt
      721 tgatggtaat gtaacaataa cttctgatat taccggatta ctgaacagtt cttccaaact
      781 tgggttaagt atgcataaac atagtgaaag aaaatcttta tattctgagg cagatgcatt
      841 aattgcagtt ggcaaaggta ataaggaaag tattatagag caaattagta agtacagaga
      901 agaaggattt cctgataatt ttggattggc tgaagccacg gttattttag ttgatttaaa
      961 taattcaatc gctgagaatc ttctagattt gtggtggaga gagtatttga actcagaaag
     1021 tgggcgagat cagctcgcat ttccttacgt gttgtggaag tccggacatg caatgaagga
     1081 agtgactacc ttaggcaata atgtatataa aagcgctaaa ttaagaatca taagtcatta
     1141 ttagccctaa atgatttcta aactattctt tcaaatccta gtaaatattt ggagatagaa
     1201 gatttccttt ttaaagtcat aaaaaattaa gttatatttt ttgagtgaat ggagaagacg
     1261 catagtatca taaacttcgt tatatcttaa attatggggt atgcctaaat aataaatgta
     1321 atacgagtat aaagaagaaa gcaattttgg gtttaaccct attggttcct ttcagaaaaa
     1381 ggtggatgaa taaaaatgaa aatattaata acaggtggag caggatttat tggttttcat
     1441 ttgtctagaa aattactcaa ggaaactgaa aatcaaattg tagcgattga taatctcaat
     1501 gattattatg atgtccaatt aaaagaagat cgattaacga tactgtacga ttataataat
     1561 tttaaattta tcagaatgga tctattagag aaagaaaaat tagattctct atttgaaagt
     1621 gaaggttttg acatagtaat tcatttagct gctcaagcag gtgttagata ttctattgaa
     1681 aacccttatg cttatattga ttctaatatt atcggttttc ttaatattct tgaagcctgc
     1741 cgaaacttcc ctgtaaagca tctattatat gcatcatcta gttcagtata tggtgggaac
     1801 acgaaagtac cgttttcaac agaagattcg gtggatcatc cagttagcct ttatgcggct
     1861 accaaaaaat cgaatgaatt aatggctcat acttatagcc acctttataa aataccaaca
     1921 actgggctga gatttttcac tgtctatggt ccatttgggc gaccagatat ggcctatttc
     1981 tcttttacca agaatatttt atctggaaaa gaaattaaga tttttaacca tggaaagatg
     2041 gaaagagatt tcacttacat cgatgatatc gtagagggaa tcactagatt aatcaataaa
     2101 gtacctgaac ccaactccga ctggacagac atcgatgatt tgagtagtga aagttgggca
     2161 ccatataaga tatacaatat tggaaataat aagccggttg cattgttgga gtttgttgaa
     2221 acaattgaaa agtatttagg tgttaaagcg aacaagattt ttttggacat gcagccgggt
     2281 gatgtcgtta gaacttatgc cgatattgaa gatttagagg aaaaaatcaa ctttaaaccg
     2341 aatataacct tggatgaggg cattaaaaaa ttcgttgaat ggtatacagt gtattatttg
     2401 agttaatttt gaaaggaata ttgaagatgg gtaattcata cgattttttt agctttgtta
     2461 ggtattttag gaaattctac tttcaaacgt gacgaacttt ctcgccgaat agatattttc
     2521 tataactaaa agaatttcta taaaacaaaa ttgtgtatca agatgtgtga actggattct
     2581 gactgccaat attgattgat gagattgaag aaatatatag ttgatcatgg atatctttat
     2641 ttgaaaagat cttagctatt cacatattca aaaaaatttt tactacgagg actcacacga
     2701 tacagaggaa tttaggtagt tctaattaaa ccagaaagtc atctatagat gtgagttgag
     2761 ggaggtcata cggttggata gtagcagtaa taaatcacat tcaatagcta aaaaagcttt
     2821 tatttacgca ataggaacat tatcatcaaa aatgatatct gtcattataa tccctatcta
     2881 tgcagtatat atttctgttg ccgatttagg aagctttgat tatcaaaata caattgctca
     2941 atttctttct ccagttattt ttggagcaat atgggaagca attttacgca tgggattagg
     3001 caaagaaaaa aatattcaga aaaaagtagt gacgaacagt atttgttttg ttatagcaaa
     3061 tacagtgata ttagccattt ttttaccaat aatatataga atgattttcc cgaatttaga
     3121 gtattatcaa ttgtttgttt ttcaattaat tttcactcct atcctaagtg tttttcaata
     3181 tattgtaaga gcactaggtg aatcaaagat ttttgtgatg agtggtataa tcgctgcggg
     3241 tatcaacctg ttactcctta ctatttttgt tgttacaatg ggtatgggga tacttggtct
     3301 cttactctct atcataattt ctaatgttat aaatttgatt tttttgattt tcagggggca
     3361 gatctttcaa tatttcgcaa cgagttcttt tgatttttta tttttaaagt ccatgctgaa
     3421 atactctatc ccactcactt tgaatctcac ttttggatgg ctaatagttg gtttttcaag
     3481 attttatatt ggaacgtaca tcggagtaac agaaaatggt attttttctt ttgcgatgaa
     3541 attttcatct ataattttaa ctcttacaag tattgttagt atgtctatga tagaggatgc
     3601 ggttatcacg tctgagaatg atgatttcat cgaaagattc gttgagaaaa atacctatat
     3661 ttttaaaatg tacattaggt tagcgactct ttctattcct atcatttgct gtgcatactc
     3721 ttttataacc aaccctgatt ataaaaaatc attagttcta gttccaatat tagttattat
     3781 ggcatgtttc agtgggatga caacaaatat cggaaatata tttgcagtat acaataaaac
     3841 aaatcagtta ttaatgattt cagtagtgtc aggtctattg agtgttgttg gcacctattt
     3901 attaggctat tcttttggag taattggtgt ctcatttgct actttgctcg gtactttagt
     3961 attatttgga cttagatatt attttggaag gaaaattgct tattatgcat ttgattggaa
     4021 gcgaatatcg ctacttttag ttctctattc cgcaatcgct tttgtatgca ttcatagagg
     4081 cttctatttt aatcttttct taacgattat ttttagtgct attttcgttt ttacgtataa
     4141 acaattaatt ataacgtatc taaaaaaatt tttcagaata aaaatggcta aaaggtgagg
     4201 gtatacgaag taagaataga atctttcgaa gaataaagag aaaactattt ggaaaaaatc
     4261 tgcgagaatg tattaatgaa ggtttgatat tcggaaaaaa acatagttta gtagaggttc
     4321 ctgaatttgg gagcgagcca tatttaattg aaatagggga taatttcaga tgttcatttg
     4381 gtgttaaatt tataacacac gatggtggag tctgggtctt aaggaagatg agttctgtga
     4441 gcgagtcaac ttcaaaaata ggtaggatca aattgggaaa taatattttt attggtattt
     4501 actctattgt tttcccagga gtttcaattg gcgataatgt aattattaga gcgaacactt
     4561 tagtaaataa agatatccca aataatagtg ttgtagcggg tagttcatgt aaa
//
LOCUS       DAYYAJ010000170         4466 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000170 DAYYAJ010000000
VERSION     DAYYAJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.172"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1056..1979
                     /locus_tag="ACHATZ_002784"
     CDS             1056..1979
                     /locus_tag="ACHATZ_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8405243.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1995..2534
                     /locus_tag="ACHATZ_002785"
     CDS             1995..2534
                     /locus_tag="ACHATZ_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8405244.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3215..3388
                     /locus_tag="ACHATZ_002786"
     CDS             3215..3388
                     /locus_tag="ACHATZ_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405245.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            3793..4227
                     /gene="bacA"
                     /locus_tag="ACHATZ_002787"
     CDS             3793..4227
                     /gene="bacA"
                     /locus_tag="ACHATZ_002787"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8405246.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1663 a          667 c          760 g         1376 t
ORIGIN      
        1 cattttctag ccactttttg tattgtggag cgtgggtttt gagggggatt caagggggag
       61 cttgcgcccc tttgacaagc ggttatttgc accaaatacg gaggtatttg gctagcaaat
      121 atagagcttg ccaaaccctt ttagtgagtt ttgtagaaag tacctttaaa ggtactttca
      181 cattacgaac gatacgtccg taggacgtac tgaaagggtg ggacttatga gcaaattaat
      241 gcaaaatttg gctagcaaaa aagctaaaaa atatatgcta ataatcgagc gaaataaaca
      301 ctttttcaag tgagcgaatc cgccatgaca aagctgaagc agtttatttc agctttgtca
      361 tggcgtgtgc cgactttttt aaaagtctag gcacacgagc aaatatcttt atcaagctct
      421 aatttgcgtt ttaagacgtt tgaaacgcct aagtatataa atatataaga ataggtttaa
      481 gagtgcttaa atcgaatttt agagggcatt tagacaattt tagggttgtt agggcttgcc
      541 ctgaccgcct gtaaggcgtt ttaagaacaa gataagggat ttatactagg actgtcttaa
      601 aatgtcttaa acgaacgctc agagcttctc aaagggttcc ttatgaacgg cttttagcaa
      661 aggagccgga ggcgactgcg cagtataatg tgagagcaga gcgatcacac ctttttagcg
      721 aaccgtaggg agcaaggttt ggaattggca gttttgataa attcgcgaac ctgtttagag
      781 aaaaaaggag tgatttttta ggcgggaaaa ttagcctccc ctctttttat atctcttttt
      841 atatctcttt ttatatctct tttatgtcgg ctgtgtcctt actctcccaa gcaattcaga
      901 gttttttcaa gtaggttttc tacgcttttc aagtaggttt tctacgcttt tcaagtaggt
      961 tttctacgct tttcaagtag gttttccgac attattgagt atttttataa agtatgttac
     1021 ttttatttca tactttataa aagtaggaga agcttatggc aaatgaaatt gtaaaatatg
     1081 aaaatagatt aaatcatatt cctctgagaa aatttaatag tcgtgaaatg aacttatttt
     1141 tttcaattgc ttcacgtgta cgtgaaaaag gtgtagatga gattattttt tcatttcacg
     1201 atttgaaaaa actaagcggt tatacagaac gtggggaaca atttataaaa gatcttagta
     1261 gcacgtatga caaattatta agtattaatg cttggaatga tgatggaaat actttaacta
     1321 aatttgttgc tttcacaaaa tatagcatta tacgagataa acaacttgtt ataatttcag
     1381 ttaatccaga atttaaagga ctttttaatc aattaaagaa ctggacaaga tttaatctag
     1441 agcaatttac taatttggat agcacatatt caaaaactat gtttcgatta ttaaaacaat
     1501 atcgaactca aggctggact gaattttcca aagaaaaatt ttttgattta ctagatattc
     1561 ctaaaagtta ttggaatagt ccgtcaaata ttgacaaatt tatcttaagg ccaattaaag
     1621 aagaactcac tcccttattt aaaggattaa ctattcgtaa aaaatatggt aaaggtcgag
     1681 gtaaacctgt tatcggttat cgttttactt ggaaagctga aattaaccat gctgacgatt
     1741 tttccaaagg gaaacaagaa gatttacgta taaaattatt taatattgat cacaatggag
     1801 aattaacacg agaagaaaaa tggcgcgcta aagatcgaat actaaatctt cccttaggca
     1861 ctcatgaagc tgattttaaa caacaacaac aaactgaaga aaaacaggca gaaaaacgag
     1921 aactttcaaa caaactcaaa caagatttac taggaaattt acaaaactta ttcgattaag
     1981 cttaaggagt atttatggca gataaaacaa tcaaagaact tgcggaagaa ttaggagtta
     2041 gtaaaacagc aattaacaaa aaagtttctg acaaaaatcg aaaactttgg tttgcaaaaa
     2101 acggaaacaa gtttgtgatt aacgaaaccg gtcaaaaagc tataaaatca atgtttttag
     2161 tcgataacgc aaacttaaat cgaaaactag ttggcgaaaa atcaaaaacc aaaaaccata
     2221 aaaacaccaa ccaagtttcc gaattaaaac tcctttcttt cctagaaaaa cagttaaatc
     2281 gaaaagatat acagctacaa gaaaaagata atcaaataaa acaaatgcaa aaattactcg
     2341 accagcaaca acaactcact ctacaagcga accaacaaat tgagaaccta caagaacagc
     2401 tccatttaac ttatacagaa gaaactccaa caaccgaatc aactgccttt tctgaaaaaa
     2461 aagcagacaa tacacaacca cttattgaaa aaaagtggtg gcaaatttgg aaatctaata
     2521 gaaccaatct ctaaaatttt ctagaagtta gttcggatag tagaggaaaa agttgtacta
     2581 cctgatgggg agatcactca aatttttatg agaaagaaaa aatagtttac aactgtaaac
     2641 aatcaatgtt tatagttgta aactatcaaa ctatatggtg acttattgct taaaataggt
     2701 tgaaatctca ctcaattttt ggtggaaaaa caaaataaat tctaatcgat tttgttgaaa
     2761 aagtcataaa aaaacaaatg tgattttaac aaaatttcaa aaaaatcgtt tgaaatttcg
     2821 tttatttttt ttgagaattt tgatcatcgt agggtcattg tagaatcatt atgagatcat
     2881 cgtgggatca tcgaataaag tatgggaaag gcttgcggtg cttggtatta agggcattct
     2941 ccgcctccca ggttcttgac cattttctct tataagtggg atttttacca cgttgagaga
     3001 aaatggtcaa gaacctggga ggcggagaaa acataccaag caccacaagc aaaaaaagtt
     3061 agagtaactt ccaaaaaaga aaaatttttt agaacaactt ttgaaaattt aattttgatt
     3121 tatatccgtt caatttaaac atttgataaa ctgaaggcta gtatttaaaa tgtgtataca
     3181 agtggtagcg ttaaaaaaag tggaggtata acctatgtta aaaaaaactg tgttggtact
     3241 gggttctatt tctctaggaa tattaggttt tagccccatg ctattatccg taattggaaa
     3301 aaacaattcc aacagagaac ttaaattgaa ttcaaattat tacaacagtc ctaacgaaga
     3361 attgtttgta aaagaaacgg ataaataata aaaaagactg ctcttccgag cagccagtta
     3421 aaaaataatg agtgtttatt cttgaaaaag caacgcgctt ataaagcgct accaaaaatt
     3481 tatcatattt tatgaaaaat aacaatccag taaaaaatga ctggtaaaaa ttcacaaaga
     3541 aacggaaaga agcaactgca gaaaatcaag ccttaaaaag agctttttac ctagaccaat
     3601 taaaaatcac aaagtgagcg tctctatcca ttggtagagg ggctacttgg tctagtcgac
     3661 attatgtcta ctaaccttta aaataacacg ttttataaaa taaaccctct tgacaatatt
     3721 taattttctg attattctaa atatatacag aaagcgcttt cctaggattt tatatctaag
     3781 gaggaagtaa caatgaaaaa taaaattatt aaaattatta cttgtctatt tttgttcggt
     3841 atgattgttg gagcaagtac tccgttaaat ctgtttcctt ttagtgctag aggtataaaa
     3901 gtggaagctg caagttcacg atataatcat aatcatcgtg gatttacttg ctataaaatg
     3961 aactattatg ttactaataa aatgtgtaga gatttgaaaa aaaattataa taagctaaag
     4021 actcctgctc aaattgcatc ttttattcct attggtggag ttggaacatg ggttattaca
     4081 aatgctttta gtagtgctgt tgatgcaatg aatgtctttg taagagctgc aaatcaagga
     4141 aaaggtgtac aattaacata taatgctcac tttagcaata caacatcata tcaatacaat
     4201 gattacgctc gttatgtaat aaaataatca gaaagtgaaa ctatctcatg aataaaaaga
     4261 ttttagctgt aattatttca ataatagttt taatcactat gttcttcata tttaggctaa
     4321 ttttcaatat aagcttacaa aaaagtattc tttatctaat tcctatagca tttatcttag
     4381 caatttttag aagtatttac ggaaataaaa actctaaata gctaaaaata aatcatacta
     4441 aattttacaa aaagtggcta gcattt
//
LOCUS       DAYYAJ010000171         4424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000171 DAYYAJ010000000
VERSION     DAYYAJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.173"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..2689
                     /locus_tag="ACHATZ_002788"
     CDS             20..2689
                     /locus_tag="ACHATZ_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8405247.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2780..3394)
                     /locus_tag="ACHATZ_002789"
     CDS             complement(2780..3394)
                     /locus_tag="ACHATZ_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8405248.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3514..4053
                     /locus_tag="ACHATZ_002790"
     CDS             3514..4053
                     /locus_tag="ACHATZ_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405249.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4050..4388
                     /locus_tag="ACHATZ_002791"
     CDS             4050..4388
                     /locus_tag="ACHATZ_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8405250.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1367 a          772 c          835 g         1450 t
ORIGIN      
        1 aatttggtgg aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg
       61 ctacgcagca gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat
      121 cgggatctat cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc
      181 taattttcat gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg
      241 gaatgcttct ctaggattga attatttatc tttaatttct tactatctag gcgggatctt
      301 cacgccactg gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac
      361 tttgcttaag gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat
      421 tcctcgctgg gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc
      481 acattcggag atcatcatgt ggctggatgc atttgtttat cttccattag tgattttggg
      541 gattcatcgt gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt
      601 gtttttatcc agtttttata tgggttttat gattggcgta ttttctttct tatattttat
      661 cgcccgtcta ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac
      721 ttctttactg gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg
      781 gtcaaatggc gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct
      841 ggatctaatc atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc
      901 gttcgtttac gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa
      961 agtaccgttg aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt
     1021 ctatcttgtt ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt
     1081 ccgttatgcc tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa
     1141 gatagaacaa gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt
     1201 tgcggtggca tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt
     1261 tgtattgaca gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa
     1321 aaaactttca ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact
     1381 ggtcaatacg aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag
     1441 tttatatact gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc
     1501 cgactcgttt taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta
     1561 tggttacagt ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct
     1621 ggataaatta ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac
     1681 tttgctaatg gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt
     1741 cagtaaatat ggcttccgcc aagctggtca aagtggcgac tatacactgt atcagaatgt
     1801 caatgcattg ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt
     1861 gacagataat ttgaccagtc agacaaactt gatcaataca ttgtctggtt taaatgaaca
     1921 gtattttaca ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac
     1981 aacgactggc gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga
     2041 agtccatgtg cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcaca
     2101 attagaaagt tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa
     2161 catttctggt caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac
     2221 tgcaagtttt tatggaacga aagaagtaag tttcatggag cctaaagtcg ttggtctaga
     2281 tgttgtagcc tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc
     2341 cactggcaga aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac
     2401 tattccttat gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa
     2461 tttcaaagac gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag
     2521 ttacttgcct gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt
     2581 tattctttat gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc
     2641 aatagaagaa aaaccgcgaa atacacacag aagaagacga agaaaataaa gaaacgatgg
     2701 aatctgtgtc actcttctat ttaaatggca aaaatttcaa tctttaaatg acaaaaacca
     2761 cctgattttt ggtggttttt tatataccta tgactaaact atctcttgct gcttcgacaa
     2821 cttctacagt gatcttatcc atttgattga ttcttaaaat ccgatcaatc tgcgcaaata
     2881 aacgatgaat aagcctaaaa tttccttttg tgatcttaat gatagttgtg atagcctcat
     2941 aatcagaaaa atcttctagc ttgatatcaa ttcctaaatc tgcccatttg tattccaaaa
     3001 tatgatgtgt ttcgtcctta ctaaggttat caaattcatg tgcaaatcct atgcgagagt
     3061 aaagctgcgg ataacgagac aatctttttt caataccagg cataccgata agaaccattg
     3121 ccagatcatt tctatcgtaa atatctctta gctgttctaa atgctgaagc tttaaacgat
     3181 caatttcatc gacaataatt aagtcaattg cttcataggt tttaggcaca ggaggatctt
     3241 cttgattgta ttttagtacc atatgcattg ctttagtcag acctattcta ctagttattg
     3301 tgttaatatc cgaactgaca cgagacatct tttcagctgg tacagtataa aaaactttat
     3361 ttacacttaa tatttcatct gttgcattta tcaagaacaa gaaaaagtct gaatattcaa
     3421 aaaaaataac ggtgtacatt tttaaaagga gatgtataat cgagatttag aaagcgtttt
     3481 cctcaaaatt taatgaatta ggaagtgatt actatgaaaa aattatttag cgcacttttt
     3541 attctgttat ttggctttat tggtattggg acttctggaa atatagttaa agctagcacg
     3601 atggttgaac aaacaagtga aatggcaaaa acttttagtc aattaacacc agaagaacaa
     3661 agttacttca aatccaaagg atttagtgat aaagatactt tttacactca aaagatagag
     3721 cctcaagatc cgaaaacacg tttttctgca aatgtattgc ggttaaccgc atctactaaa
     3781 aaaattacta atacgcaggg gcggacagaa tatattataa caagtcccaa ttcggcgatg
     3841 tatcgtattt ctacacgctt gagtttaggt agtgcaaaat catatactca aacagtaaat
     3901 ctagctaatt cttatggtta tggtggagaa atgtatttta catttactgg gaaaagacaa
     3961 tattacagtg taaaattagc tatgcaagtt tataatacat ttggtgtagg gtcagtctct
     4021 tgcactgcgg gaggattgac aataggaaaa tgataatttc ttactatgtc ttaaatagaa
     4081 gtgatgatga aggactcttc gtttttcaat cattacatga ggatacgatg ctaaaagagt
     4141 tttataatcg gattaagaat aaacagatag aaacaaattt atatttttta agtgactcta
     4201 tcttgccttt tattagcaac cctaaaattc ttaacttgtt gcagacaaaa gatttgaaaa
     4261 gtttaccgat tattgtcatt gatgaggaaa ttgtaaaata tggcgcttat ctaagcgtag
     4321 aagaattaga aaaattaact aacattggaa tgagtattca ggaggataat ggggtacatg
     4381 gaacatagaa tgaatatgca cagttgagaa cggaatttga catt
//
LOCUS       DAYYAJ010000172         4343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000172 DAYYAJ010000000
VERSION     DAYYAJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.174"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..848
                     /locus_tag="ACHATZ_002792"
     CDS             147..848
                     /locus_tag="ACHATZ_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8405251.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1420..2436)
                     /gene="tsaD"
                     /locus_tag="ACHATZ_002793"
     CDS             complement(1420..2436)
                     /gene="tsaD"
                     /locus_tag="ACHATZ_002793"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8405252.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2433..2897)
                     /gene="rimI"
                     /locus_tag="ACHATZ_002794"
     CDS             complement(2433..2897)
                     /gene="rimI"
                     /locus_tag="ACHATZ_002794"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8405253.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2904..3446)
                     /gene="rimI"
                     /locus_tag="ACHATZ_002795"
     CDS             complement(2904..3446)
                     /gene="rimI"
                     /locus_tag="ACHATZ_002795"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8405254.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3430..4152)
                     /gene="tsaB"
                     /locus_tag="ACHATZ_002796"
     CDS             complement(3430..4152)
                     /gene="tsaB"
                     /locus_tag="ACHATZ_002796"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8405255.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1289 a          921 c          743 g         1390 t
ORIGIN      
        1 caataccatt gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg
       61 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
      121 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
      181 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
      241 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
      301 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
      361 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
      421 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
      481 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
      541 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
      601 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
      661 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
      721 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccggac
      781 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
      841 gagaataacg cgaacaaaaa taagcagtga ccctgatatt tatcagggtc actgcttatt
      901 ttcctcccta aaacttctga aagatccggc agttttagat cactgggcaa gactcaaaga
      961 ttttctatgt gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgatagatta
     1021 actagaggaa gcgtcttgtc ctcatccgac ctagattatt cgatattcct cgacaataaa
     1081 cgattaatca gtgagggcaa tcagaggaac ggataatgat gaataagctg taaaaaatag
     1141 ggctttgaca aaagcgtgtc acagccctta tttcgattaa acggtgtgta cctgtcaact
     1201 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
     1261 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
     1321 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
     1381 tcttattact tgatgcaggg cagatttttc acaacctctt tattcagata aaaccaagct
     1441 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
     1501 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
     1561 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
     1621 tacacctcca gctacaatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
     1681 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
     1741 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
     1801 aaaactgaaa tcataattat cttcatggat catcgctcgt ggaaaatggt aattatcttt
     1861 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1921 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1981 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     2041 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     2101 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     2161 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     2221 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     2281 gcggctagct acttcaggaa ctacgccacc aaaacgttta tgactgttta tttgagaagc
     2341 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     2401 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     2461 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     2521 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     2581 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     2641 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     2701 attactttcg agtatccaat aaagccacag atctggttat cttcaactaa aagcagataa
     2761 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     2821 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2881 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2941 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa acctaacttc
     3001 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     3061 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     3121 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     3181 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     3241 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     3301 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     3361 cgatcgattt gttcgattgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     3421 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     3481 tttttcttct gcttctaccc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     3541 tggttcagct aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     3601 aactgtatta atcatcgcac taggcaatat ttcctttatt tcatagaaga atttgtgaca
     3661 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     3721 agcagaaata tgtacatctt caagtacaat ttctaatcta ttttctttcc agcggtatgc
     3781 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataattt tatccacacc
     3841 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3901 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3961 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     4021 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     4081 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     4141 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     4201 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaatgat acatgattat
     4261 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcacttcat
     4321 ttccccacag cttttccatc ttt
//
LOCUS       DAYYAJ010000173         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000173 DAYYAJ010000000
VERSION     DAYYAJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.175"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHATZ_002797"
     CDS             127..579
                     /locus_tag="ACHATZ_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405256.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHATZ_002798"
     CDS             593..1282
                     /locus_tag="ACHATZ_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405257.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHATZ_002799"
     CDS             1407..3758
                     /locus_tag="ACHATZ_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8405258.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHATZ_002800"
     CDS             3838..4233
                     /locus_tag="ACHATZ_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405259.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DAYYAJ010000174         4305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000174 DAYYAJ010000000
VERSION     DAYYAJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.176"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1137
                     /locus_tag="ACHATZ_002801"
     CDS             148..1137
                     /locus_tag="ACHATZ_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFM8405260.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="ACHATZ_002802"
     CDS             1134..1553
                     /locus_tag="ACHATZ_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405261.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="ACHATZ_002803"
     CDS             1562..1870
                     /locus_tag="ACHATZ_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405262.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="ACHATZ_002804"
     CDS             1874..2236
                     /locus_tag="ACHATZ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405263.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="ACHATZ_002805"
     CDS             2458..3864
                     /locus_tag="ACHATZ_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFM8405264.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DAYYAJ010000175         4257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000175 DAYYAJ010000000
VERSION     DAYYAJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.177"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1086
                     /locus_tag="ACHATZ_002806"
     CDS             112..1086
                     /locus_tag="ACHATZ_002806"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8405265.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1068..1529
                     /locus_tag="ACHATZ_002807"
     CDS             1068..1529
                     /locus_tag="ACHATZ_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8405266.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1579..2043
                     /locus_tag="ACHATZ_002808"
     CDS             1579..2043
                     /locus_tag="ACHATZ_002808"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8405267.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2057..2806
                     /locus_tag="ACHATZ_002809"
     CDS             2057..2806
                     /locus_tag="ACHATZ_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8405268.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2806..3636
                     /locus_tag="ACHATZ_002810"
     CDS             2806..3636
                     /locus_tag="ACHATZ_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8405269.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3636..>4257
                     /locus_tag="ACHATZ_002811"
     CDS             3636..>4257
                     /locus_tag="ACHATZ_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8405270.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQ"
BASE COUNT         1463 a          552 c          814 g         1428 t
ORIGIN      
        1 ataaaaaaat ttttttgaaa aatagtattg caaaataaag caatttcata gataataggt
       61 attgtaaagt tatatgactt tataatactt attagagtga gggataaaga aatgagacaa
      121 ctacacaaag aacagattga aacagcaatg gcagcgttaa aggaaaagac aattgatcat
      181 gtatatttta cagcatgtgg gggttcacaa gccgttttaa tggctggtca atatctattt
      241 gataaagaat taactgtacc ttgtcatgtt tatactgcta acgaatttaa ctatgctata
      301 ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt cccattctgg cacaacacca
      361 gaaactgtag aagcaacaaa aaaagcaaga gaatttggag caatgactat ttgtttatct
      421 aatgaagaag gatctcctct atgggaagca gctgaatatc ctgttcacta tgattggggt
      481 aaagatgttg atgctagtga tttaaataaa ggaattcttt atggtttatt atttaatatt
      541 ttacttgttt tagatggctc taaaaaatgg gaaacttgct tgaatgaatt tgataagtta
      601 aatgatcttg ttattgctga aaagagcaaa tttgaaataa ctgctaaaga atggggtaaa
      661 aaccaaaaac gagataaaat tatttacaca attggtagcg gtatcaatta cggagaaatt
      721 tactccacag cagcttgctg gttcatggaa atgcaatgga ttaattctaa tgctattcat
      781 tctggggaat ttttccatgg tccttttgaa atcactgatt atgatgtacc attcatctta
      841 gtgaaaagta ttggagatac aagattttta gatcaacggg tagaagactt cactaaaaaa
      901 ttcagtaata aattaatggt tttagaccaa gctgattttg acttagaatc tatagatagt
      961 gtagcgaaag aatatattgc agctgtactt agtggcgttg ttattcgcca aatggtagaa
     1021 gcgattgctt ttgaaagagg ccattcctta gatgttagac gctatatgtg gcaaatgaaa
     1081 tattaattaa taggaggttg caaaagatgg caaatcaatt atttcacatt gtgattacaa
     1141 cacattctac cctttgtgag ggttatttaa aggcaacaaa attaatttta atgaatgacg
     1201 ccacaggtat tagtgctgtt ccatttaaag aaaatactag tccagatgat tttgaggaga
     1261 gtatgaaaga aataattagt cagcatcatt tgcaacctct aattatttta acggatttaa
     1321 ttggaggaac acctaataat gtttctatta aacagttgaa caacaataga attcaaatta
     1381 ttgctgggat taatttacct cttttactgg agttattaat ggcccagtca gctggtaaat
     1441 ctatagaaga aatagatttt aaggaattac tgaataactc tcaaaaaagt atggttcata
     1501 ttaattcgct tttaaaagat gtcagttaag gtggcaattt ctattctata aattttaaaa
     1561 attgaaggag gaaatagaat gattaaatta gtgcgtgtag accacagatt acttcatgga
     1621 caagtaatat tttcttggac taaacagtat gatattaacc atattattgt ggctgataac
     1681 aaagttccag gagatccaat gtctgtgatg gctttgacaa ttgctaaacc tgctgattgc
     1741 aaattagaca ttgttaaaat tagtgatgtt aaagggatta tggaaacatc cccaaatgat
     1801 aattacatga ttttaattaa aggacctaag gaagcttttg aattagttca aaatttacca
     1861 gaaattaaag aaattaattt tggcggtgtg tctaagaaaa gcgagtccaa acaatatgga
     1921 aaggcagtct ttttaactcc tgaagaatta gaagccacaa aaaaaattct tgatcaagaa
     1981 gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg aaaatgtaga ttttagtaag
     2041 taagggggga aaataaatgt tattaaaagc cattttatta ggtttagttg gtgtggtagc
     2101 tgttattgac tccaggttaa ttggtcgaca aaatattggt agaccactaa ttttgagtac
     2161 acttgccgga cttgtgctag gtgacttgca tacagggatt gttcttgggg catctttaga
     2221 attaatttct atgggattcg tctcaattgg ggcggctgga cctcctaata tgcaacttgg
     2281 tagtattatt gccactgctt ttgcaatttt aactaatagt agtacagaaa cagccttaac
     2341 aatagctatc ccagtggctg tagctggtga atttatgagt attatcatgc gcatgataat
     2401 tgctcaattt ggacatgctg cagataaagc cattgaaaat aataaattta gaaaagctca
     2461 aattattcat atttattggt catttatttt taatgctttt gtttacttta ttccaatctt
     2521 tttaacagta tattttggtg ctgacgttgt ggcagattta gtagataaaa ttccagaaat
     2581 gattacagac gccttgactg tctctggaaa tatgctttcc gcattagggt ttgcaatgct
     2641 attaagcaca atgttgagta aaaaattata tccttacttt atttttggat tctttattgt
     2701 tgcctattca ggtctttcac taattggcgt gacaattttt gcagctctga ttgcctttgt
     2761 aatggatcaa gttaaatatg gaaaaagaga agaggcgcat ggttaatgga aaatagtaca
     2821 gaaaaaaaac gtttaatcac aaaaaaagat cttaataaga tgtgtcttcg ttcatggaca
     2881 atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg gatttatgta tgggatggcg
     2941 ccaattttaa ataaagttta taaaaatgat gaagagaaaa aaagagaagc ttatactcgt
     3001 catatggcat tttttaactg tacgcctcaa atgacttcat ttattatggg actttgtgct
     3061 tctatggaag aacaaaatgc tcaagcagaa gaaggaaact ttaatactga atctatctcc
     3121 atgattaaaa ccagtttaat gggtcctttt gctggaattg gtgattcatt tttccaaggt
     3181 actttaagaa ttattacatt tgggattgga ttgtcttttg ctaagcaagg tagtatttta
     3241 ggaccgattt tagctgttgg cctttttgct ataccttcta ttttagctgt tttttacggc
     3301 ggtaaattag gatatgagtc tggcaataaa taccttacta aattgtatca agaaggtctt
     3361 atggatcgag taatgcaatt tgcttctatc attggtttgg ctgtagtagg tggaatggtg
     3421 gctagcatgg tgccaattgt caccccttta aaattcacta cagggggaac agaacttgtt
     3481 ttacaagact tacttgattc cattattcct ggattattac cacttgtatg tactttggga
     3541 atatataaat tgattaagaa aaatgttagt acaaatatgc tcttattagg ttgtattttg
     3601 gcagggtttg ttttaagtat aataggtatc ttataatgat taaggctcta atttttgata
     3661 tggacggagt tttaattgat agcgagatta tgtatcaaaa gtgggtattt aattttttaa
     3721 aacaagaaaa tttaagctat cctttgtatt cttatcaaaa aaagattgga actacagcaa
     3781 gtatttttga tgatatagaa aagtacaatg agggatgtaa cagtttagaa ttgaagggaa
     3841 aatttactaa atattggaaa tcgcaaaaga ttgattactt ggatattttt cctaatatac
     3901 taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa aatggcaatt gcttcttcca
     3961 gtccaatgga tactataaat gaagttgtta ccacttgtaa aatagagtca tattttgatt
     4021 gtttaattag cggaagagat ttgccagaaa gtaaacctaa tcctactata ttccttaaag
     4081 cttcagaaca gctcagaatt ccagttgaag aatgtctggt gatagaagat tcttataatg
     4141 gtataaaagc tggtaaaaga gcaaatatga aggttcttgc aattaaagat aaacgttttt
     4201 ctcaagatgt aagtctagca gatgatgtta tttacgatat taaatatctg cgacaag
//
LOCUS       DAYYAJ010000176         4085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000176 DAYYAJ010000000
VERSION     DAYYAJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.180"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..113)
                     /gene="ffs"
                     /locus_tag="ACHATZ_002812"
     ncRNA           complement(29..113)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHATZ_002812"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(136..651)
                     /gene="tadA"
                     /locus_tag="ACHATZ_002813"
     CDS             complement(136..651)
                     /gene="tadA"
                     /locus_tag="ACHATZ_002813"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8405271.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            757..1614
                     /locus_tag="ACHATZ_002814"
     CDS             757..1614
                     /locus_tag="ACHATZ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405272.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(1695..2030)
                     /locus_tag="ACHATZ_002815"
     CDS             complement(1695..2030)
                     /locus_tag="ACHATZ_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405273.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(2097..3467)
                     /locus_tag="ACHATZ_002816"
     CDS             complement(2097..3467)
                     /locus_tag="ACHATZ_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8405274.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(3545..4081)
                     /locus_tag="ACHATZ_002817"
     CDS             complement(3545..4081)
                     /locus_tag="ACHATZ_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8405275.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         1167 a          891 c          645 g         1382 t
ORIGIN      
        1 acagacccga aagtctgttg tcgtttcatt ataatcagac acatggcgga acctgcttag
       61 tgctgcttcc ttccagacct gacacgcttc acaagcccac cattgtccta gcctttcggc
      121 aaagaatagt ataccttatt tttcattcgt tgaccagtct ttttttaatt tcttttttcg
      181 ctggcggagc tttcggaaaa agtcagtcaa aatcatccca cattcgtctt ctaaaatgcc
      241 accttctaca tatgcttggt ggttaaaccg ctcgtcctcc aaaagattca tcaaggtacc
      301 ggctgttccg ccttttggat catacgcgcc gaaataaact tctgctacac gagacaaaat
      361 catcgcgccg ctgcacatag gacaaggctc taaggtaaca aaaagtgcag catcttccaa
      421 gcgccagctt cccacttttt cacaggcttc cttgattgca agcatttctg catgtgtagt
      481 agcatcttgc gtggtttctc taaggttata tcctcttcca atcacttttc catccaatac
      541 aaccactgct ccaatcggaa cttcatgaag catttctgct ttctttgctt ctgccaatgc
      601 aaggcgcatc catttttctt tttctactgc atcaagattt gtatttacca attacccttc
      661 cctgcctttg ttttttcttg ttttacttta gcatatgata aaaaggctgt aactaacttt
      721 ttttctatgg taaaataaat acaagaggtg aacaagatga aaatggataa attacttgct
      781 ctttacccac aagcacagaa aaaaaactct ccagcatcag aagcggatat cctttctttt
      841 gcaattgatg atgcttttat ctggatcaaa caagattcat tatctcaaca agaaaccagc
      901 cttttgaaag cgttattccc tgtaatcaac gatcaaaaaa aacacccatg gtatcactat
      961 ctttttaaag atgcttcttg tgttcaagaa aaaagctttc gtttgatcca gcttcacgtc
     1021 gaatcaaaag gagaattctt aaaaaaagaa tggcaagata ctattttgga attatttcgc
     1081 tattctgctg atttcttttt ttatacagac acagatgcgc tcttagtcga acaaataaac
     1141 cgtgattatc ttgatgctac tgaaatcaac ggtatttttc tatctttaga tgctgatttt
     1201 gatacgacaa cgtctgtatt cgttggttct ttccattcac ctggcaccga cctcgtccct
     1261 ttgtttgctg aagaaagaaa aattttttta acagagcaaa acaatcttta tactcacagc
     1321 cgtacttttt ctatgcaaga tgtggcttta cattactata caaaagatac tgtaaaagat
     1381 agtaagttga tcaaaagtta catagaactg atcaaacaaa atgaaatgga agtgatcatt
     1441 caagaattat ggaatgaatt aggcaatgtc agctctgctg caaaaacact gtttcttcat
     1501 cgtaatactc taaaatatcg aatcgaaaaa tttcaagaac agtctggttt caatctaaaa
     1561 aaagccaacg atttactgtt ttgctatctt ttattgcttc atgaacataa ttaaagatag
     1621 attaaagggg ttgtgacaat aattttgtca caacccctta tttcgaatga atagtgttca
     1681 aaagctagtt tctcttacat cattttttca acaaaatgca ttagccggtc ttttacatct
     1741 tcggttgaat ctttgattga atcaccataa acagagattt tcttttttcc tgatttgatt
     1801 tttgctaaca ttcccatcaa agaatccaag tcactatctg aaagaccatc tacgatttct
     1861 agtaattttt catttccatc aaatttgtca tagacgaatt tttttacttt cgtccgagta
     1921 gtcatatgat ggatcttttc cattactttt cccgatacaa cgactgctgt cgctacactg
     1981 gcagcggctg ctacgcttaa tccgattgtt acttttgttg atgttttcat aagtatcagc
     2041 tcctttttat ctatctccat catagcatag acaaaaaaat aaacgaacta atccactcag
     2101 tgaaatctca atcctttagg gaaaaagttc tttactgttc cttttacttg tttcccaaat
     2161 ccgacaggta tcttatctaa tactagcaaa acccatcctt gattcccatc acgttgaaat
     2221 gtttctcctg ctgtgtaaga ttgccattcc ttttgagtga tcggcaagca aggtatattc
     2281 tctatctttt ttgtagcaag cgctaatgca tagctaggtt caaatcgatt ctttttgaaa
     2341 tctcctaaat gcaaacctgt acgtacgact tttaaaccat cgagagaagg ggctaactca
     2401 ggaacttccc aaagatggtc gttgaacact aaaagtcttc cagtagcttc ataatgaaaa
     2461 tcatttgaaa actccgtcca gagtttttcc tgctcttttg tcatttgcac tttacttttt
     2521 tttcgtgtct tcttttcttt atgcatctga ttttgtccat gaaaagtcag ctttgcaaca
     2581 aaatgtcctt caccctgatc tttatgaggc catatacgta ttgttttttc caaaccagct
     2641 acgcttcccc attcagaacg tcctgaacta acggattgag tcaaaggaat ctcctcaatc
     2701 gttactggat aattttctac aagccagcta atgatctctt cattttcttc tggtgcaaac
     2761 gtacaagtcg aataaatcag ttgtccttta ttttttagca tttttattgc tgaagacaaa
     2821 atctcttgct gacgtttttg acagtatagc ggagattctt ctgtccactc ttttattgca
     2881 tttggatctt ttcggaacat tccttctcct gaacaaggag catcaaccac gatccgatca
     2941 aaaaagcccg aaaaatgagg gacaagttct gcgggtgcat gatttgtcac tattgcgttc
     3001 gaaactcccc atcgttctat attttctgat aaaatcttcg cacgtttcgg aaagatctca
     3061 ttcgtcacta ataacccttt ccccttcatt tgagcagcta attgggttga tttaccaccg
     3121 ggagccgcac acaaatccaa aactttttca ccgggttttg ctgcagcagc tgttccaacg
     3181 atcattgcac ttggttcttg gctatattca tacccagctt gatggaggaa agactttcca
     3241 ttgacagtgc caagaaatcc ctcattgctg tagggagcgg gttgtaattc ttcgctatgg
     3301 taagtttgga ccatgtccaa tcctgccggt tttagtggat tccaccgaaa tccttttttc
     3361 acgcttcctt gttcaagcgc agaaaaaaaa tcggatgctt cttcgcctaa aagaagacga
     3421 tattttttga taaattgctg tggtaacgtt gtcgcttcct ccttcattcc aatgtttcgt
     3481 tagacatttt acaatctttt ttacgaaaaa taaaggtggc tgatctactt tttcatgttg
     3541 ctgcctaagg tgactgttcc tttaagatat tttccaagta ttcatccgtc acctcgtttt
     3601 tatcccaaac agcgttcatc atttcaagaa aatcttgaga agaatcatct gagcgcaaac
     3661 ttcccaatgc attaagccat accaattcaa gcgacttttc ttctttgatc ttcgtgtgac
     3721 ctttgacata tgagaaaaat ggccgcttaa atagcgaatc catgtctaaa tcagattttg
     3781 caatgatttc acgaatactt tcatggatag aaaaaaaatc tttcaataag tagacaatgc
     3841 ttaaaacatc atcggaactg cattcagcta agtagttttc aaaataaaca gtgatttctt
     3901 ctaatatctt ctcttttaac ccgctgattg atttgaaatg tgtataaatc gggtatgtag
     3961 aacaaccgca gaactctgct aactctcttg cagtaattga cttcattcca tattcttcca
     4021 cgtataccat acttttgaac agcaaatatt ctttactata atttgttact ctccccatca
     4081 ttctt
//
LOCUS       DAYYAJ010000177         4081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000177 DAYYAJ010000000
VERSION     DAYYAJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.181"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..687
                     /locus_tag="ACHATZ_002818"
     CDS             <1..687
                     /locus_tag="ACHATZ_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8405276.1"
                     /translation="YDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGD
                     TIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGI
                     VYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEM
                     HITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQL
                     GLVDNFNNIS"
     gene            704..1138
                     /locus_tag="ACHATZ_002819"
     CDS             704..1138
                     /locus_tag="ACHATZ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405277.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1755..>4081
                     /gene="esp"
                     /locus_tag="ACHATZ_002820"
     CDS             1755..>4081
                     /gene="esp"
                     /locus_tag="ACHATZ_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8405278.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1495 a          568 c          776 g         1242 t
ORIGIN      
        1 tacgatccag aaatatatct gtttctttat gaaaataaaa tactggaata tgttaaaaat
       61 ggagatattc gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt
      121 gttagtggag atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta
      181 gcacaaacgt ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct
      241 ttgatacaag aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct
      301 gggattgttt attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta
      361 atttcttctg tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa
      421 atagctaatt atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg
      481 catattacga tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg
      541 aaatcaaacc atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac
      601 ttttcgagag tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta
      661 gtggacaatt tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt
      721 acgtagtttg gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc
      781 tgatttgagt tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa
      841 tgaatatgac aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc
      901 tatcagtcat attaatggta gcaattttaa acaaacatta tttaatctga ttaagctaga
      961 tgctaaaata tcaagttatt tattcttttt gatacatgat gaatttatgg attatcaaaa
     1021 attggatcag ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc
     1081 agaaaagtta aacaactaca agttaataga ttataagtat ttgagtgaaa gcaattaatt
     1141 attcatgtag agtactagac actttaaata tggacaattc aattataaag aaaaagaatc
     1201 aataatttaa tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa
     1261 atgttgataa ctccaatcat tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa
     1321 ccgatacttt ataattataa agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca
     1381 cctttttact tatgtcacag tctcattcag taatgcggga taaggctcct tattccgtta
     1441 tttgaatact ctgaagttta atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc
     1501 atagaaatga acttaatttc agaaagaata catacatatc tttttatatg ttttcagctg
     1561 ctgaatggtt aaaaagcaca aatatccgaa aaaaacatga agaaatgtac tttttttgtt
     1621 gtaatgttca tgaaattctc taagtaaatt agttaacata aggttagtaa gattcctatt
     1681 ctgtgaataa tacgtatcag atttatcttt tattcttact aggaaagtaa ttattaaggg
     1741 agttgatttg aataatggtt agcaagaata ataagagagt atttctagaa aaaacaaaga
     1801 aacgagtatt gaagtacagt atcaaaaaat taagtgttgg ggttgcatct gttttgattg
     1861 gtgttggtct tgtcttggga acaactgaac tagttaaagc acaagatgaa atttctccaa
     1921 gcagcccact tgaaacagcg ataagctccg tacaagtagg tgacaaagta gcttcgggga
     1981 atacttttca ggagaatcct ggttatacga aaaattataa cttttcggat ttacaattta
     2041 gtcctcaaga actaactgga gacacactaa aaggcaatac aattggattt gaagtttacg
     2101 gaaaacataa tattgcagca tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac
     2161 gattagctaa atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta
     2221 gacggacctt tgtaagaatt aatgattcgc tgggtagacc tacaaatatt tggaaggtca
     2281 attatattcg tgcaagtgat ggattatttg ctggggcaga aacaaccgat acgcaaaccg
     2341 ctcctaatgg ggtaattacg tttgaaaaga gcctagatga aatttttaaa gagataggca
     2401 tagacaatct caagaccgat cgtttaatgt atcgaatcta cttggtaagt catcaagacg
     2461 atgataaaat tgtaccgggt attgatagta cgggctactt tttaacagat tcagatgatt
     2521 tctataatag cttagatgta tctgaaaata atcctgacca gtttaaacat ggatcagtaa
     2581 gtgcaaaata tgagaaacct aatactcaaa caaaagatgg ttcaggatcc acaggtgcta
     2641 atggagctat aatcttagat cataagttaa cgaaaaatta taatttctca tattcatctt
     2701 ccgcaaaggg cacgccttgg tatgctaatt acaaaattga tgaacgatta gttccatatg
     2761 tagcgggtat acagatgcac atggttcagg ctgacaaagt aacatatgat gtttcgtttg
     2821 aatccggaac aaaagtagct gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg
     2881 gcataggttc aataactaat aatgatttaa ctaaacttat cgattttgct aatgcaagtc
     2941 cacgtccagt cgttattcga tatgttttac aattgactaa gccattagat gaaatcttag
     3001 aagatatgaa agcaacagca caagttgaag aaaataaacc atttggtgaa gatttcatct
     3061 ttgattcttg gttgtcggat acgaataaaa aattaatcca gaacacttat ggaacaggtt
     3121 attattattt gcaagatatt gatggtgatg gaaaccctga cgataaagaa gagagcggag
     3181 acacgaatcc atatatcggg aaacctgaat tagaagaagt atatgatgtt gacacaacag
     3241 ttaaggggaa agtattcatc cacgagttag cgggaacagg tcacaaagcc caacttgttg
     3301 ataaagaagg tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc
     3361 caatttcaga tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga
     3421 aagatgaatt aaaaatccaa atcgtttctc caggttttga taaaccagaa gaaggttcaa
     3481 ccgttattaa ggaatcacca aaagcggttg ataaacaaac cgtggtagtt ggatttaaac
     3541 cagatgctaa agaatcaatt cggaataata aaaacttacc tgaagatgca gagtattcat
     3601 ggaaaacaga gcctgatact tctaacgtta ctgatagtac gaaaggtatt gtaactgtta
     3661 agatcggaaa tcgaactttc gacgtggatg tagagtttgc tgtaaaagct tctcaagcta
     3721 tggaaaatga tgcaacatac gtacctataa caacaactcc agaaacgaca gttcaaagtg
     3781 gaaaacctac atttgataaa ccagatgttc ctttagctaa agatgccttt tcaatttcag
     3841 atgtctataa taagtacttc ggtaatgcaa gtgttgatgc aaacactggt attgttacat
     3901 tcactccagc taaaggtgta ggagaatcgg agccgattac tggaacaatt cctattaaaa
     3961 ttgtttacca agatggttct gtaggcacga ccgatttagc agtaactgta agtaaagata
     4021 tttatgaaaa tccaggagaa aacattcctg caggctacca caaagtaacc ttcaccgcag
     4081 g
//
LOCUS       DAYYAJ010000178         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000178 DAYYAJ010000000
VERSION     DAYYAJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.182"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHATZ_002821"
     CDS             85..1434
                     /locus_tag="ACHATZ_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8405279.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHATZ_002822"
     CDS             complement(1562..2152)
                     /locus_tag="ACHATZ_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8405280.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHATZ_002823"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHATZ_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8405281.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYAJ010000179         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000179 DAYYAJ010000000
VERSION     DAYYAJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.183"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACHATZ_002824"
     CDS             complement(<1..1157)
                     /locus_tag="ACHATZ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8405282.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACHATZ_002825"
     CDS             complement(1160..3409)
                     /locus_tag="ACHATZ_002825"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8405283.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACHATZ_002826"
     CDS             complement(3506..4009)
                     /locus_tag="ACHATZ_002826"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405284.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          743 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttatta
//
LOCUS       DAYYAJ010000180         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000180 DAYYAJ010000000
VERSION     DAYYAJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.185"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHATZ_002827"
     CDS             complement(717..3866)
                     /locus_tag="ACHATZ_002827"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8405285.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYAJ010000181         3864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000181 DAYYAJ010000000
VERSION     DAYYAJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.186"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..606)
                     /locus_tag="ACHATZ_002828"
     CDS             complement(64..606)
                     /locus_tag="ACHATZ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8405286.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="ACHATZ_002829"
     CDS             741..1130
                     /locus_tag="ACHATZ_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405287.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="ACHATZ_002830"
     CDS             1134..1898
                     /locus_tag="ACHATZ_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8405288.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="ACHATZ_002831"
     CDS             2027..2974
                     /locus_tag="ACHATZ_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8405289.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="ACHATZ_002832"
     CDS             3104..3844
                     /locus_tag="ACHATZ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405290.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1286 a          657 c          787 g         1134 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaataca actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tatt
//
LOCUS       DAYYAJ010000182         3740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000182 DAYYAJ010000000
VERSION     DAYYAJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.187"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACHATZ_002833"
     CDS             complement(<1..434)
                     /locus_tag="ACHATZ_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFM8405291.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYL"
     gene            complement(424..1623)
                     /locus_tag="ACHATZ_002834"
     CDS             complement(424..1623)
                     /locus_tag="ACHATZ_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8405292.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1753..3129)
                     /locus_tag="ACHATZ_002835"
     CDS             complement(1753..3129)
                     /locus_tag="ACHATZ_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8405293.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3129..>3740)
                     /locus_tag="ACHATZ_002836"
     CDS             complement(3129..>3740)
                     /locus_tag="ACHATZ_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8405294.1"
                     /translation="SKEKVLSGVNQKFELGKFYAIVGKSGTGKSTLLSLLAGLDKPQT
                     GKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIRLVNKSADESILFELG
                     LDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTL
                     AKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         1153 a          709 c          535 g         1343 t
ORIGIN      
        1 agatacaaat tatcctctgt tacaaaacct gctttaactg cttgttctaa aatataggca
       61 aaaatatcct caaacaccgt cgtttctcta aatcggcgaa cataattctt tccaaaagtt
      121 gagaaatggg ggattttctc ctcgaaggag tagcctaaga accagcggta agcgacattg
      181 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
      241 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      301 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      361 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      421 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      481 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      541 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      601 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      661 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      721 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      781 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      841 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      901 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      961 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
     1021 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
     1081 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
     1141 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1201 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1261 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1321 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1381 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1441 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1501 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1561 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1621 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1681 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1741 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1801 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1861 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1921 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1981 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     2041 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     2101 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     2161 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2221 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2281 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2341 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2401 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2461 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2521 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2581 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2641 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2701 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2761 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2821 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2881 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2941 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     3001 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     3061 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     3121 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     3181 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     3241 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     3301 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     3361 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3421 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3481 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
     3541 atattatttt ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat
     3601 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
     3661 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
     3721 gacaaaactt tttctttaga
//
LOCUS       DAYYAJ010000183         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000183 DAYYAJ010000000
VERSION     DAYYAJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.188"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHATZ_002837"
     CDS             complement(<1..353)
                     /locus_tag="ACHATZ_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405295.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHATZ_002838"
     CDS             complement(613..1161)
                     /locus_tag="ACHATZ_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405296.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHATZ_002839"
     CDS             complement(1162..2019)
                     /locus_tag="ACHATZ_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8405297.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHATZ_002840"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHATZ_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHATZ_002841"
     CDS             complement(2581..2910)
                     /locus_tag="ACHATZ_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8405298.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHATZ_002842"
     CDS             complement(2987..3259)
                     /locus_tag="ACHATZ_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8405299.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHATZ_002843"
     CDS             complement(3259..3522)
                     /locus_tag="ACHATZ_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8405300.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYAJ010000184         3717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000184 DAYYAJ010000000
VERSION     DAYYAJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.189"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHATZ_002844"
     CDS             72..155
                     /locus_tag="ACHATZ_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8405301.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHATZ_002845"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHATZ_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8405302.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHATZ_002846"
     CDS             complement(1280..2143)
                     /locus_tag="ACHATZ_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8405303.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHATZ_002847"
     CDS             complement(2145..2417)
                     /locus_tag="ACHATZ_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8405304.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHATZ_002848"
     CDS             complement(2434..2643)
                     /locus_tag="ACHATZ_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8405305.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHATZ_002849"
     CDS             complement(2721..3617)
                     /locus_tag="ACHATZ_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8405306.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAYYAJ010000185         3599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000185 DAYYAJ010000000
VERSION     DAYYAJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.190"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..228)
                     /locus_tag="ACHATZ_002850"
     CDS             complement(<1..228)
                     /locus_tag="ACHATZ_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405307.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEF"
     gene            complement(526..1500)
                     /gene="sgrA"
                     /locus_tag="ACHATZ_002851"
     CDS             complement(526..1500)
                     /gene="sgrA"
                     /locus_tag="ACHATZ_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8405308.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1696..2535)
                     /locus_tag="ACHATZ_002852"
     CDS             complement(1696..2535)
                     /locus_tag="ACHATZ_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8405309.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2720..>3599
                     /locus_tag="ACHATZ_002853"
     CDS             2720..>3599
                     /locus_tag="ACHATZ_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8405310.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNI"
BASE COUNT         1204 a          737 c          529 g         1129 t
ORIGIN      
        1 aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt ctggatacct
       61 ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg tgtagtttag
      121 atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac aatagttcaa
      181 aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag tacatactcc
      241 tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca aatagattcg
      301 ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt
      361 attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa acaaaaatag
      421 atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa tattcactca
      481 aaaatctatc gttttattca attttagatc tgttgctttt attctttatt tcactcgatg
      541 gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac ccatccatgt
      601 aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc tattatctgt
      661 ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct gttttagcac
      721 actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga taggtaccaa
      781 atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact
      841 ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
      901 gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
      961 gttttctgtg ctaatttcac tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc
     1021 tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa ctgtccaact
     1081 gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac
     1141 tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta ttgtatactc
     1201 aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt tttctgaact
     1261 tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat tcacgctttt
     1321 gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa tccgatcagt
     1381 tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac tgattccaat
     1441 cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg ttttcttcat
     1501 ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata aaatgaaaat
     1561 gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa
     1621 tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg atatcagcct
     1681 tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct ttgttttata
     1741 tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg atatagatgc
     1801 ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca gagtggagaa
     1861 aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt gtgtccaatt
     1921 ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga taagtgcgct
     1981 gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt taggattcaa
     2041 tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg gaccaaatct
     2101 aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc ccactgcaat
     2161 aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat ctaaccgata
     2221 cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc caacaacgag
     2281 acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat cttctattct
     2341 ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta aatgtccgac
     2401 cgtaccggca attgctccaa tccattcact atttccaata aacatatccg caagtgcatt
     2461 attgacaaga attcctaaaa caaaaccaac aaagactatt aaaacagatc caatcaaccg
     2521 aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc ttttctttct
     2581 tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt ttacgaacta
     2641 agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct tctgttagac
     2701 tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa ttatgtgcct
     2761 ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa cgattgcaac
     2821 gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag ttaaatcaaa
     2881 taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg tatacggaaa
     2941 agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa aactcggtcc
     3001 aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata agaaactctt
     3061 taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa cagataaaga
     3121 tctagacact gcttggaaag aattctatcc agaagttagt acacagatca ttctattttc
     3181 gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa atttctccaa
     3241 gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa aaatgaattt
     3301 tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta aactaaaaaa
     3361 cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc aaagctatta
     3421 tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat acaaatctga
     3481 gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg ataaaacaat
     3541 ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa aaaatatat
//
LOCUS       DAYYAJ010000186         3579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000186 DAYYAJ010000000
VERSION     DAYYAJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.191"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..1422
                     /locus_tag="ACHATZ_002854"
     CDS             91..1422
                     /locus_tag="ACHATZ_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8405311.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1388..1738
                     /locus_tag="ACHATZ_002855"
     CDS             1388..1738
                     /locus_tag="ACHATZ_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405312.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1830..2249)
                     /locus_tag="ACHATZ_002856"
     CDS             complement(1830..2249)
                     /locus_tag="ACHATZ_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8405313.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2250..2594)
                     /locus_tag="ACHATZ_002857"
     CDS             complement(2250..2594)
                     /locus_tag="ACHATZ_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8405314.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2944..3540
                     /locus_tag="ACHATZ_002858"
     CDS             2944..3540
                     /locus_tag="ACHATZ_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8405315.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          941 a          651 c          648 g         1339 t
ORIGIN      
        1 cccttacatt tttagttttg acctaaatga tagttgtcca aatctttcat ttcaagttat
       61 agttaaagaa aatgaaaaag aaggtgtgta atggaaaacg aaactgacgt tatttatatt
      121 catcctcaaa aacgaatcgt ttcacagaaa agaaagtatt tttatcttgg ttttacaggt
      181 gttttttttc tttttattgg cttgctttct aatactccta ctgataattg gagtggattg
      241 ctaactattc ttacctcgcc aagcaatctt ttgacagatt attttgcttt aggtgttttc
      301 ggaagcgcat tcataaatgt agggattttg accttacttt ctgtgctttt agcttatcgg
      361 cacaaggtga ttttaaacgg tcccttattt gcttcgattc taacagttac cggtttctct
      421 ttttttggca aaaaccttta taattctatc tcaatcattt taggtgtgta tctatacgct
      481 gtatttgtca ataaaccttt ttctcagtac atcatgatcg gactgtttgg tagcgtatta
      541 tcaccggtcg ttagttatat cacttttggt atgcgttttc ctttactgat aggtatttta
      601 ttggggaatt tagctggcat agcaattggt atacttcttc cgcccttagc tgctcaaact
      661 ttagttttcc atcgtggatt cacactttat aatattggat tcacctctgg gttgattgcg
      721 atgactttta ctgctgtttt gaggttattc agctattcaa tcgtcgaaaa tacattggtt
      781 ttcaatgaat atcactttcc tctcatttgg attatttttg gctttttttc gctgacagtc
      841 ggaattggtt tctattataa ttcctttcgg ctttcgggga ttcgagaaat tttcgattca
      901 tctggaaaat tgacaactga tttcattgct aattctggta ttggtgctac tttgatcaac
      961 atgggcttag ttggtctgat gctgtcttct tatgttttgc tggttggcgg tcagttaaat
     1021 ggacctgtca tcggtgctat tttatctgcg gtgggtttca gtgcttttgg ttgtcattta
     1081 aaaaacagtt ttccgatact agtcggaata tttattgctt ctttgtttgg aacttttcat
     1141 gagatcacca gtactggcat gcttgttgca gctgtgtttg ggacaggtct tgcgcccata
     1201 agcggttttt acggcagttt ttacggtgta atagctggtg tgctacatat tgctcttgtc
     1261 cataacgtca gcactttgca cggaggacta aatctatata acagtggttt ttcaactggt
     1321 ttcgttgcag gtatccttgt accgatacta gataacttta ccgctgtcag aaaggaaaaa
     1381 aagacacttg gaaaaagaat tatcaaaaag aatcatcgat gagcttctta cttatttata
     1441 ttcccatgaa ataactgaaa tacgtattgg agtagaattt gctgaggaag ggcttttcat
     1501 agagatacag ggtacggcag aaactcagcc agaagattta gatgtacttg ctgatttgct
     1561 gaacaatcct cgggacccta gtatggaaat ctactatcaa gaactattag gtaatccccg
     1621 acatgatcgg gaagattatc atctgttagg tagtttgatt gatgacgtgg aaatcatgta
     1681 tgcccagccg attatcaata ttaagatctt tcgacgtgct tctcattcct attattaacc
     1741 ctattttcta tttataaaag actgaatcaa aaaagcagct cctgaaagtt cgattaaaat
     1801 ctactttcag gagctgttca agcttgacat tattgatttt ccaatccttc atacacttca
     1861 tctgttacag gttctaaaaa ctctgaatta ccagcagtga tcgccaaatg ttcaaaccaa
     1921 ctgtcttttg tcgcaccatg ccaatgttta atccctttgt gcgttacgac gacatctcca
     1981 gccattaatc gtcttgctgg ctttccttct tcctgatacc agccttctcc tccggtcaca
     2041 agtaagattt gatagccgtc tgtatggaca tgccagtgat tgcgacaacc tggttcaaaa
     2101 gttacattac ctacaccaac agaaacttcg ggatcattca ctaacatatt cagatagctt
     2161 tgcccggtaa atacttgagc gtaagcttcg tttttttcac ctgctggaaa aatcacgcca
     2221 ttgtgtattt cttcgtgttt tgtcatcatt tacgcctctt tcttatcttc gctattttct
     2281 tctaaataga tttcttttgc taaactaaaa gctgaccaag cttttggcca ttctacataa
     2341 aaagctaaat gggtaatgat ttctgctatt tcttcactag taatgccatt ttgcttgcca
     2401 atcttcagat gagctggcaa ttgttcaaaa tttcctcctg aaattaatgc agatatggtg
     2461 atgatactac gctgatgagc agataattct tctctggacc atacttgtcc aaataaaaca
     2521 tcatcattta attctgcaaa ttttggtgca aaatctccta attgatctcg tcctgcggtt
     2581 tgtttttctg ccatttctct tattctccct tcacgactgt taggtgattc aactatggtt
     2641 ctaaggcaac tttattttct attgtttctt cgcatttcat tttaaatgta gtctttcagc
     2701 ctgtccaatg cttagtttat tcagtcgata tgctttttaa gcatagttgc taacgctttt
     2761 tttattttaa aaaaaacaag ttattaaata agtctttagt tggatgtttt tttgagatca
     2821 atcttttata cttatgatga acttacaaaa ttgtttgttt tctgtcatat caaaaaatgg
     2881 cgcagttagt tgcttttcta gtaaagtata gttaacaaag aacctctaag gagttgatga
     2941 ataatgaata ttgctgcctc tcgaaaactg ataaatttca tatccattat cggattaggc
     3001 ctatcaatcg cgctgacgat ttattttatt aatttaggtg ttttcaaaga tttgaatgcg
     3061 ttaagaggat tagtaggtga ttccatcatt ttaggaccga ttatttttgt cttcatccaa
     3121 atcctgcaag tagttattcc aatcattcct ggagggatca gtactgcagc tggtgtcctg
     3181 atatttggcc cttatgctgg atttatctat aactatattg gaatctgcat tggttcgatt
     3241 atcatttttc tgctagggcg tcgttatgga aagccattca tactttccat gattagcgat
     3301 aagacatata acaaatatat tggctggctg gataatcaaa accgttttga gaagctgttt
     3361 gctctagcta tttttcttcc agttgctcct gacgatgcgt tatgtttgat ggctggattg
     3421 acaaacatgt cagtgaaacg atatacacta atcattttga ttgctaaacc actgtcgatt
     3481 ttcttatata gtatggcctt gatttatgga ggtcaatatc taagtggtct tcttggataa
     3541 tatttatttt gtaaaattag tcattgattg acaatgtta
//
LOCUS       DAYYAJ010000187         3530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000187 DAYYAJ010000000
VERSION     DAYYAJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.192"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2129
                     /locus_tag="ACHATZ_002859"
     CDS             <1..2129
                     /locus_tag="ACHATZ_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8405316.1"
                     /translation="KVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDA
                     KWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKE
                     DLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTV
                     EGEKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGT
                     KDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTP
                     GGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGT
                     VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEV
                     TDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKK
                     GTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDL
                     PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITE
                     GDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGN
                     TSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNI
                     FTISGGLILLGTLGLLGYKNRKKENE"
     gene            2263..>3530
                     /locus_tag="ACHATZ_002860"
     CDS             2263..>3530
                     /locus_tag="ACHATZ_002860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8405317.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLD"
BASE COUNT         1394 a          502 c          722 g          912 t
ORIGIN      
        1 acaaagtaac cttcaccgca ggagaaggaa caagtattga aagtggaaca acagtctttg
       61 cagtgaaaga cggcgtaagc ttaccagaag ataaacttcc agtgttgaaa gcaaaagatg
      121 gttatacaga tgcgaaatgg ccagaggaag caactcaacc aattacagca gatgatacag
      181 aatttgtatc aagtgcaaca aaactggatg ataaatctga cgctgacaaa tataatcctg
      241 aaggtcaaaa agtgactaca gaattgaata aggaaccaga cgcatttgag ggaattaaga
      301 acaaagaaga tttaccaaaa gatactaagt atacttggaa agaaaaagta gatgttagcg
      361 cagctggaaa taaaaaaggg actgttgtag tgacatatcc agatggatca tctgatgaag
      421 ttgaagtaga tgttacagtg acagacaatc gttctgacgc cgataaatat gagccaacag
      481 tagaaggcga aaaagtagaa gttggaggca cagtagatct aacagataat gtaaccaacc
      541 taccaacatt accagaagga acaacagtaa cagatgtcac tcctggtggt acgattgata
      601 ccaacacacc aggtaattac gaaggggtta tcgaagtaac gtatccagat ggtacgaaag
      661 atacagtgaa agttccagta gaagtgacag acaatcgttc cgatgccgat aaatatgaac
      721 caacagtaga aggcgaaaaa gtagaagttg ggggcacagt agatctaaca gataatgtta
      781 ctaacttgcc agcgttacca gaaggaacaa cagtaacaga tgttactccc ggcggtacaa
      841 ttgataccaa cacaccaggt aattacgaag gtgtcattga agtaacgtat ccagacggta
      901 cgaaagatac ggtgaaagtt ccagtagaag tgacagacaa tcgttctgac gccgataaat
      961 atgaaccaac agtagaaggc gaaaaagtag aaattggagg cacagtagat ttaacagata
     1021 atgttactaa cttgccagcg ttaccagaag gaacaacagt aacagatgtt actcccggcg
     1081 gtacaattga taccaacaca ccaggcaatt atgaaggtgt cattgaagta acgtatccag
     1141 acggtacgaa agatacggtg aaagttccag tagaagtgac agacaatcgt tctaatgcag
     1201 ataaatacaa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
     1261 ctgagggaat taagaacaaa gaagatttac caaaaggaac tacttatgct tggaaagaga
     1321 aagtagatgt tagcacagct ggaaataaaa aaggtactat tgtagtgaca tatccagatg
     1381 gatcatctga tgaagttgaa gtagacgtta cagtgacaga tactcgttcc gatgcagata
     1441 aatataatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca gatgcatctg
     1501 agggaattaa gaacaaagaa gatctaccaa aaggaactac ttatacttgg aaagaaaaag
     1561 tagatgttag tacagctgga aacaaaaaag gtactgtagt agtaacatat ccagatggat
     1621 ctaaagaaga agtagaagtt actatttctg tagtagataa aaaagcaccg aataaacctc
     1681 aagttgatcc tattacagag ggtgaccaaa ttgttactgg taaaactgaa ccaaatgcag
     1741 aggtgacagt tacattacca gacggaagtc aacaccatgg gacagctgat aaaaatggta
     1801 attttacagt taaagttcct aaattagagg cagggacaaa agtgatagta actgcaactg
     1861 atgaatctgg taatactagt gaacctacta atgtagttgt ttcttccaac aaaaaagaca
     1921 gtggaaaaaa cggtagtaaa ggtagtaaga ctgacaatca aggtagcaac agcaatcaag
     1981 acaaaaatag aggcaaaagc cagtcctcaa aagtttttcc taagacggga gagagtgaca
     2041 gtaatatctt tactattagc ggtggtttaa tactgttagg aacgttagga ttattagggt
     2101 ataaaaatcg aaaaaaagag aatgaataga aaatcaattt cttaagtctt ctctgagaag
     2161 aggaagaatc tttttcttcc tctttttgct tttttatgat gggaaataag tgttagttaa
     2221 agatgaatat actttttata gaaaggaaaa gaatgtaatt aagtggaaaa aatagtaatt
     2281 attggttcaa accattcagg gctagccgca gctcgatttc taaacttaag cgattcaaat
     2341 tatgaagtta ttctaatcga taaaaaatca gaaattagtt acttggaatg tgggacatcg
     2401 ttatttataa aaggagaagt aagtaattta gaagatcttt tttatcaaaa tagaatgaca
     2461 ctaaattctg agataaaaaa gttatttcta gagacagaag ttgacaaaat tgatattcaa
     2521 aataaaaaga ttagttgtgt aaaaaaagat ggtacaagat tctctcaaga atatgacaaa
     2581 gttatattag ctacaggttc aaagcaaatt atgctagata taccggggaa taatttagct
     2641 aatatttttt cgataaagaa ttttccaaat gcaaaagaga tttttaaagc tttggatgat
     2701 agtactatta aaaaagtagg aattattgga gcaggatata ttggagttga aattgctgaa
     2761 gccataagaa aaagagggaa agaagtatat cttttcgatg ttgcagatag agtgttgtcg
     2821 acttattacg accgtagttt ttcagataaa gttgaagaaa tactatcaaa aaatggaata
     2881 catctttgtc tatcagaaag tcctaaaaaa tatttaggaa gaaaaaagat tgaaaaaata
     2941 gtcactacaa gtggtaccca atactctatg gatatggtcg tacaagctat aggatttcta
     3001 tctaatagtc caattgggga aaaggaactc ttgagagatg ttagtggagc atatctaact
     3061 aatgaatacc agcaaacaaa tataaaagat atatatgcta ttggggattg tgctacaact
     3121 ttttttacgt ccattaatag aagtagtgtt gatttttcta tttctaatgc tctaagaaca
     3181 gcttatattg caactcaaca tattaacaat caagatttta caccttctgg ttcccaattt
     3241 acaaatggct tttctatatt taattacaat ttttacgcag caggtttgaa tttaaaaaca
     3301 gcacgattat taagtgagtc tatagattat atagaaatag aagatttcat tagacctgct
     3361 tttttgagaa aaaatagtcg tgtaaaatta agaattagct ttgaaaaaaa tacaaagaga
     3421 atagtaggtg tacaactttg tagtcaagaa gatttatctg gactattatc aatgttttct
     3481 cttgctatag aagaacaaat tacattagac aaattgaaaa tgttagatta
//
LOCUS       DAYYAJ010000188         3512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000188 DAYYAJ010000000
VERSION     DAYYAJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.193"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1011
                     /gene="galE"
                     /locus_tag="ACHATZ_002861"
     CDS             22..1011
                     /gene="galE"
                     /locus_tag="ACHATZ_002861"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8405318.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1023..2507
                     /gene="galT"
                     /locus_tag="ACHATZ_002862"
     CDS             1023..2507
                     /gene="galT"
                     /locus_tag="ACHATZ_002862"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8405319.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2531..3511
                     /locus_tag="ACHATZ_002863"
     CDS             2531..3511
                     /locus_tag="ACHATZ_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8405320.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1188 a          642 c          775 g          907 t
ORIGIN      
        1 ctaatcaaag gagtgttttt tatgacaatt ttagtactag gcggagccgg ttatatcggt
       61 tcgcacgctg tagaccagct agttcaaaag gggtatcaag tagctgtggt agataatttg
      121 ctcacaggtc acaaacaagc agtacatcca gatgctcatt tttatgaagg agacatccgg
      181 gataaagaat ttctacgttc cgtatttgaa aaggaaccga ttgaaggagt aatccatttt
      241 gctgcaagct ctttagtagg agaatctgtc gaaaaaccat tgatgtattt caacaacaac
      301 gtatacggta tgcaaatctt acttgaagtc atgcacgagt tcaatgtgaa taaaatcgtg
      361 ttctcttcta ctgcagcaac ttacggagaa ccaaaagagt ctccaatcac agaagataca
      421 ccagctaatc ctaaaaatcc gtatggtgaa agtaaattga tgatggaaaa aatgatgaag
      481 tggtgtgacc aagcatatgg catgcgctat gtcgctttgc gctacttcaa tgtagccgga
      541 gcaaaagcag atgcatctat cggagaagat catacaccag aaacgcatct tgtcccaatc
      601 attttacaag tagctcttgg tcaaagaaaa gcattggctg tttatggaga cgattatgat
      661 acaccagatg gaacatgtat cagagattat gttcaagtag aagatttgat tgctgcacat
      721 attttagctt tggaatactt gaaagaagga aatgaaagca acttcttcaa tttaggcagc
      781 agcaaaggat actcagtcaa ggaaatgcta gaagcagcta gagaagtgac aggaaaagaa
      841 attccagcag aaatagcacc aagaagagct ggagatccta gccgattagt cgcttcaagt
      901 gaaaaagcga gagaggttct gggttggaaa cctgaataca cagatatcaa agcaattatc
      961 aaaacagcat gggattggca tgtcagccat ccaaatggtt atgaagaata ggggaatgag
     1021 atatgtcaat cagtcaaacg atttgtgatt ttgccacact tgcgatcgaa gctggcggat
     1081 ggatggaaat ggaccgctta tatttacaga atcgtctttt agggatggta ggagaagatg
     1141 ctctagaaag cattgaagta agaccagtta aggaagaatc tgtagactta ttggatcagc
     1201 ttgtcgctaa ggcaagagaa aatggtgttc tttcagaatt gccagcagaa caagagattt
     1261 ttgaagcgca attaatggat tttctcacac cgccaccttc agtcgtcaat gcattctttg
     1321 cacagcacta ttcgaaaagc ccagaggaag caactgagta tttctttaat ttaagtaaga
     1381 aaaatgacta catcaaaaca agagccatcg caaaaaacat caaatttccg gcagagtcgg
     1441 agtatggtga actggaaatc acaatcaatt tatctaagcc agaaaaagat ccaaaacaaa
     1501 tcgcagcaga acgcgaggcg gtaaaagtag actatcctac ctgcatgctt tgcatggaaa
     1561 acgaaggata tagaggtcgc ttgaactatc cagcccgcac caatcaccgg attattcgaa
     1621 tgaatctaga cggtgaaagc tggggattcc aatattctcc ttatgcttat tacaatgaac
     1681 attcgatcat tttatcagaa caacaccgcc cgatgaaaat cacaaaggaa acatttgcta
     1741 gactgcttcg gattactgaa gtcttgcctc attactttgt tggttcgaat gctgacttgc
     1801 cgattgttgg aggatcgatt ctttctcatg atcattatca ggcaggacgt catgaatttc
     1861 ctatggcaaa agcaccgata gaaaaactta tcaaactagc agattatccg caagtcatcg
     1921 caggagtcgt gaaatggccg atgtctgtga tccgtttgca atcaacagag aaaaaattgt
     1981 tgattgaagc agctaactca gtattggaaa agtggaaagc atattcggat gaaacagtat
     2041 ctatcagagc tttttcagca gatggtacac cacaccatac gattacgcca attgcacgaa
     2101 gaagggacaa ttactttgag ttggaccttg tccttcgaga caacaatgtt tcagaagaac
     2161 atcctgatgg gatttttcat ccacataaag atgtccaaca tatcaaaaag gagaacattg
     2221 ggttgatcga agtaatgggg cttgctgttt taccacctcg tttgaaacca gaactagtag
     2281 aagtagagca tttcttatta ggagaagaaa accagatggc tgaatatcat cgtccgtggg
     2341 cagaacaatt aaaaaaagaa catcctgatt tgacgaaaga tacggtcacc gatgtcgtac
     2401 aaaaatctgt aggtcaggtt tttgcacgtg tactggaaga cgctggagta ttcaagcgag
     2461 atgaaaaagg acaggcagcg tttgaacgat ttactgcaat tttataaacg taaaaaagga
     2521 ggagagacaa atggcaacaa ttaaagacat tgcgcagttg gcaggagttt ctcctgctac
     2581 tgtttcaaga gttctcaact atgatcctga tctttccgtt ggcttagcca caaagcaaaa
     2641 aatttttgaa gcagcagaac gcttaaatta tacgaaacat cgtaaaaatg cagcaaatac
     2701 taagcaaacg cttcgcctga tccagtggta cgacgatacg gaagagcttg aagatcttta
     2761 ttatttatct attcgtctag gaatcgaaaa aaaggcagaa gaagcaaacg ttactttatt
     2821 gaaagaaacg tgggattcca ttagtgatct tccgacagat ggaacgatcg cactaggaaa
     2881 attcgacgaa gctcagttat tagaaatgaa aggatcccaa aatgccttgc ttttggtaga
     2941 ttctgacggc acttctcaag gaatcgactc tctagttgtc gactttaagt caagtgttca
     3001 aaaagtcatt gaacattttg tctcccataa agcgcaacat atcgctatgc ttgctggaac
     3061 agaatatacg aagaaaaacc atcagcggct aaaagatccc agaattatgg catttcaaca
     3121 agttttggca gaaaaaggct acgacgaagg tccaattatc gaagcagatt tttctgtcga
     3181 gtcaggatat caagcagtga agcagtattt aaaaacgaat ccgcatgttc ctgatgcgct
     3241 ttttgcagct aatgatgcat tagcgattgg cgcactgaaa gcgattcaag aaaaaggttt
     3301 agtggtacca gaagatattt ctgtgatggg tttcaatgat gtcagtgtag cgaaatatgt
     3361 aacgccctct ctttctactg taaaagttta taccgaatgg atgggagagc tagcagtgga
     3421 gacgattctt caattaagta attccaaagc accggtttca agaaagatta ttgtggaaac
     3481 agagttgatt attcgagaat caacgaaata aa
//
LOCUS       DAYYAJ010000189         3497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000189 DAYYAJ010000000
VERSION     DAYYAJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.195"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHATZ_002864"
     CDS             complement(<1..182)
                     /locus_tag="ACHATZ_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8405321.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHATZ_002865"
     CDS             517..951
                     /locus_tag="ACHATZ_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8405322.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHATZ_002866"
     CDS             974..1294
                     /locus_tag="ACHATZ_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405323.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHATZ_002867"
     CDS             1435..1626
                     /locus_tag="ACHATZ_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405324.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHATZ_002868"
     CDS             complement(1697..1954)
                     /locus_tag="ACHATZ_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8405325.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHATZ_002869"
     CDS             2112..2375
                     /locus_tag="ACHATZ_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405326.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHATZ_002870"
     CDS             2399..2587
                     /locus_tag="ACHATZ_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405327.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHATZ_002871"
     CDS             complement(2670..2855)
                     /locus_tag="ACHATZ_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405328.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHATZ_002872"
     CDS             3070..3441
                     /locus_tag="ACHATZ_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8405329.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1305 a          498 c          700 g          994 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgta
//
LOCUS       DAYYAJ010000190         3341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000190 DAYYAJ010000000
VERSION     DAYYAJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.196"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..121)
                     /locus_tag="ACHATZ_002873"
     tRNA            complement(49..121)
                     /locus_tag="ACHATZ_002873"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Gln,seq:ctg)
     gene            complement(167..595)
                     /locus_tag="ACHATZ_002874"
     CDS             complement(167..595)
                     /locus_tag="ACHATZ_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405330.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(655..1413)
                     /locus_tag="ACHATZ_002875"
     CDS             complement(655..1413)
                     /locus_tag="ACHATZ_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8405331.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1708..1905
                     /locus_tag="ACHATZ_002876"
     CDS             1708..1905
                     /locus_tag="ACHATZ_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405332.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2011..2493
                     /locus_tag="ACHATZ_002877"
     CDS             2011..2493
                     /locus_tag="ACHATZ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405333.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2551..3147)
                     /locus_tag="ACHATZ_002878"
     CDS             complement(2551..3147)
                     /locus_tag="ACHATZ_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8405334.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1002 a          551 c          650 g         1138 t
ORIGIN      
        1 aagattgttc ttttacaggg ggttcatttt ttcagcaagc acatcaaatt gggatggcag
       61 ggttcgaacc tacaattgct tgattcagag tcaagtgcct taccagtttg gctacatccc
      121 atttattcaa caaaataatc atagcacttt cctaaaaaaa cgcaagttat ttttcttgat
      181 cggatagttg ttgctttact cgtaaaaggc ggcgtactag tttcttttct aatccagcag
      241 gaagggcaat cagttctgct actgacaaga cggtgtccaa taactgatat tctttgacag
      301 gttcattcat aatatgatag atgtttgcta aacgttcaat atattcaaag cgagtatact
      361 ctgttcgagc aattagaaat ttcaataaat cggtggcttt ctcgtactct tgatctgcgt
      421 aataaacaag agattgacga tacaatttga tttccagttc attgagggta gcaggtctgc
      481 gacgctcgtt ccactcatta atagagactt taccttgaac atttcccttg cagtcacgga
      541 caaaaataat cggaatttca tttggatcca tttttttatg tgtgaatttt tccattatct
      601 acgctccctt caaaggtttg gtatcttgag tataagggat ttttgtttga attactagct
      661 tttctccaaa gttagcatct gtccacccat tgcagtgaaa atcgccgaaa aatcgttgat
      721 cggtactgct cgatttttta caccataggg atcgttgaca taaatatttt ctttgtcata
      781 tccagtgact acacaagcat gaacaagagg agtgactctt acttctccat tatttgtggt
      841 ccaagtcata aaatcagttt catctgggac ttcaaaattg atagtcacag aagtccagac
      901 aggatttcct ttagatagtg cttttttgat atcatcgagt tgagcatcgg tttttggaaa
      961 agtgtgataa tccgtttgga cgatttgctg agcaagggag gcaactgggg gaacatagac
     1021 gaccatagcc cagtctccgc cactgatatc accaacaaat ccttcattag gatcgccatg
     1081 agtcccatct gcattgaaaa cagggacgta atccaattgc tcagctaact ggtttttagt
     1141 tgtttcataa ccataaaaag ataaaagcat agaaagcgaa gtgacttcac agccattttc
     1201 taaagaagga gaatcaaact ggctctctaa tggaacatct agtaaaactt ctgctttctc
     1261 ttttgtcttt ttcgtttttt gtgttgaact tttttgtgca gtgttcattg tatgttctgg
     1321 agaatggata tgtaggtaat aacttgtacc gcttaataaa agagcagctg tgccccctag
     1381 aaacaatagt tttttgaacg tttttttctt catttgaaat ctcccttttc agctgtttat
     1441 ctttcctatt ataaatgtat ttttattgga aatggaagta ttatatttta atatgtagga
     1501 aataattagt ttttaaggaa tatgttttaa ttttttgaaa aaaattcttc taaatacaac
     1561 agctgtttta attgtgaaaa cgaaaacagt acgctataat gcctgtaagg ttgattttta
     1621 actgtaaata aaaatcaatg agttaaatct ccaaagaaat agcagtggta atgtggagaa
     1681 aagttccagc agtttaggag gattttgatg acggaagaaa tgattaattt aggagaacaa
     1741 tatgcatgca aaccaattgg ttttacaaaa acggtcattg gtgaagttgt atcaaaaatg
     1801 acaaactgcg ccgtggtcaa agttgcccaa tgtgctgcag aggaccaaga attattagat
     1861 gaaaaagcaa gcatggttgt tgcaaaatat gatacatttg aataaagaaa acaattggac
     1921 gtgcggcgtg ctgcaagtcc aatttctttg agaaggtaaa tcagtattct tttcctattt
     1981 tctggagaag aaaaagcgag gcgagttgtc atggtaacat tctttacggt tctattgttt
     2041 ttattcgttg ttgtgatgtt tttatttctt tggctggttc gaaaagaaat tatttatcgg
     2101 actgtgcgaa atcgatgggt ctacttggtg atacccttct tagtgtcact agtcatttgg
     2161 tatacactca tcagtcatcc tacagcagat gaattggcaa aagggatctt gtcagcaatg
     2221 atttttatta gttttttgtt ggacagtaga ggaatcacag aagaaggact tgttttgaat
     2281 agctttgaca aaaaaggtgt tcctttttct gagatcaata aaattgtatt atatcagcca
     2341 aaaggttcta aaatcgtaaa aatgaatttt ttccgaaacg gatggcgagg accgatgctg
     2401 aaattttctg catcattaga agaattagtt ccatttttgt cccaacgctt aaacgatgag
     2461 gcagagattg atattatgat tgatccagaa taaaaacaga ggttgcgaca agatgtttgt
     2521 cacaacctct gttttttatt tcaaataaaa ttatccaaat acgcggaagc gatcggcatc
     2581 gttcatgtat tctttttcct cagccggtgt ttctggtgaa ccgaatacta attgcgaacg
     2641 tagtttccag tttgaaggga tattccattc ttttgcaact gcttcgtcaa taactgggtt
     2701 gtaatgttgc aagtttgcgc ccaatccttt atcaactaat gctacccaag tattagctgt
     2761 tgcgattccg tttgattgtt ctgaccaatc tgggaagttg tctgcatata attcaaattg
     2821 ttcttgtaga ccttttacca cgtcagtatc tttgaagaat aatactgtac cataaccatt
     2881 tttgaaacca gctaatttgt tttgtgtatt tgggaaagct tctgctggag tcaatgggcg
     2941 aagtgcttct tcagtgattt cccaaagttt ttcatgtgca tctccgaaaa ggataactgc
     3001 gcgtgttgat tgagcattga aagcagttgg gctttcttta attgcttctt tgatcaaagt
     3061 tgttaattct tcattagaaa gtgaaacatt acgtcctaga tgatagattg aacggcggtt
     3121 tttcaaagtg tcagtaaaag ttgtcataat aaaatttctc ctttgatttg cgttttgcat
     3181 ttttttaatt gattacgaaa tgaagtatag caccttacaa aaagtaagta aacagatatg
     3241 ctcacaaaaa ataagcgaag ttcaaaaaac aattttaata agaaaaatcg ttatgtaagg
     3301 attttcttag ttatcgcttt cattgttgtg ttttaggaat t
//
LOCUS       DAYYAJ010000191         3341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000191 DAYYAJ010000000
VERSION     DAYYAJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.197"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(482..844)
                     /locus_tag="ACHATZ_002879"
     CDS             complement(482..844)
                     /locus_tag="ACHATZ_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8405335.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1067..1819
                     /locus_tag="ACHATZ_002880"
     CDS             1067..1819
                     /locus_tag="ACHATZ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8405336.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1959..2882)
                     /locus_tag="ACHATZ_002881"
     CDS             complement(1959..2882)
                     /locus_tag="ACHATZ_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8405337.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2897..3169)
                     /locus_tag="ACHATZ_002882"
     CDS             complement(2897..3169)
                     /locus_tag="ACHATZ_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405338.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1098 a          625 c          542 g         1076 t
ORIGIN      
        1 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
       61 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
      121 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
      181 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
      241 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
      301 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
      361 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
      421 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
      481 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
      541 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
      601 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
      661 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
      721 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
      781 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
      841 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
      901 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
      961 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
     1021 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
     1081 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
     1141 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
     1201 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
     1261 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
     1321 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
     1381 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
     1441 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
     1501 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
     1561 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
     1621 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
     1681 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
     1741 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
     1801 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
     1861 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
     1921 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
     1981 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
     2041 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
     2101 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
     2161 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
     2221 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
     2281 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
     2341 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
     2401 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
     2461 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
     2521 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
     2581 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
     2641 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
     2701 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
     2761 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
     2821 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
     2881 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
     2941 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
     3001 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
     3061 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
     3121 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
     3181 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
     3241 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
     3301 ttataatgat actgtaaata tatctttatt attagtgaat g
//
LOCUS       DAYYAJ010000192         3227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000192 DAYYAJ010000000
VERSION     DAYYAJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.198"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(551..2164)
                     /locus_tag="ACHATZ_002883"
     CDS             complement(551..2164)
                     /locus_tag="ACHATZ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8405339.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="ACHATZ_002884"
     CDS             complement(2305..3213)
                     /locus_tag="ACHATZ_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8405340.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          556 c          571 g         1062 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttt
//
LOCUS       DAYYAJ010000193         3222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000193 DAYYAJ010000000
VERSION     DAYYAJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.199"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(439..1053)
                     /locus_tag="ACHATZ_002885"
     CDS             complement(439..1053)
                     /locus_tag="ACHATZ_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8405341.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1659..2036)
                     /locus_tag="ACHATZ_002886"
     CDS             complement(1659..2036)
                     /locus_tag="ACHATZ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405342.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACHATZ_002887"
     CDS             complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACHATZ_002887"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8405343.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          978 a          660 c          499 g         1085 t
ORIGIN      
        1 tttgtgtatt ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg
       61 taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg
      121 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
      181 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      241 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      301 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      361 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      421 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      481 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      541 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      601 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      661 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      721 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      781 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      841 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      901 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      961 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
     1021 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
     1081 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1141 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1201 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1261 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1321 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1381 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1441 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1501 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1561 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1621 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1681 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1741 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1801 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1861 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1921 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     1981 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     2041 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     2101 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     2161 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     2221 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2281 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2341 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2401 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2461 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2521 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2581 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2641 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2701 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2761 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2821 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2881 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     2941 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     3001 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     3061 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     3121 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     3181 gcccagcggc aggaatgact gcttttttga ccttcacttg ct
//
LOCUS       DAYYAJ010000194         3179 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000194 DAYYAJ010000000
VERSION     DAYYAJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.200"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..493
                     /locus_tag="ACHATZ_002888"
                     /pseudo
     CDS             260..493
                     /locus_tag="ACHATZ_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(541..660)
                     /locus_tag="ACHATZ_002889"
                     /pseudo
     CDS             complement(541..660)
                     /locus_tag="ACHATZ_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(1876..3042)
                     /locus_tag="ACHATZ_002890"
     CDS             complement(1876..3042)
                     /locus_tag="ACHATZ_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8405344.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1072 a          606 c          432 g         1069 t
ORIGIN      
        1 ccaacaaatt cataggagga ttcaagatgt ctcatactct gcttatcaaa gataccctaa
       61 atattctaga cctaaatatc aatatccatt tcgaggaaaa ttgtttaact aaagaaaaat
      121 acaaaggaca aatttgcttg atttacaaag ggactcttca gtacacacca gaagaatatc
      181 cacatcttcc aatatattaa aagaaccttc ttgaatcatt gagtgaaaga aaagagattc
      241 gtcactattt gaggactgtt taactagaga cattagcgat ctgcctgaaa cctatacaac
      301 cactttacga acgtttaaaa attttctgcc acagattcaa aatgcgctta actgcagcta
      361 ttcaaacaga ccattggaat gtttaaacaa ccacatcaaa gtattaaaac gcaatacgta
      421 tggctttcgc agcttttaca atttcaaact acggatcatg attcgtcatg gcaaaacatt
      481 tttaaccaaa taaacagaag aatgtgggat aaatctaaaa atcagttttg ctacagactc
      541 tcgagtaaac ggcgggagca gaagcaactc cttcggaaat aagccgaaat tcacaaaaat
      601 ttgaaaagca attttcgtga atttcttctt atttctcgga gttaaacact tctgtcccag
      661 cctcttctgt ccaaattatt ctaagatttc tctttaccaa catcatttga caaagagctt
      721 cttttttgaa tactatattc gttctgtttt tcatcttcaa atacgaactc atccgcagaa
      781 atatttgtca caactccatg aatcttcttt tcattaccac ctatatacca caggattcct
      841 acacagaaac aacaaaaaaa tagcataaaa taggttttct ttattttata tccctccttt
      901 tagattaaaa gctcacagaa aacgctttct aaaaagacaa tagaataatc ataatttttc
      961 attttgtatt atttatacaa ataatacgca tgttactgaa aaatgatatg gttccctact
     1021 ttttgagata agttaaataa aatctgtcat cgataaaaca gctagtacag taatgataaa
     1081 tagtaagaac ttaaaatatt ttggattttt atttttgaaa taatcgaaga aaagtaaaag
     1141 tgataacgaa atagcaaaca agattatatc tataagatta ttcattgaga aatcgcccca
     1201 tatggaactc tattttgacc tgacacaccg agacataaag caatcatatc ctctgcattg
     1261 ttttatacta ttagaactat agctacacct attggatgct actggctcta agattaatgc
     1321 taatacagta acatcaatac ctaaactagc agaaactcta tttgcatctt caatttcaga
     1381 tgcctggact gtcgtgacta gcgccgtagt agaaaaattt actggtacta acaaagatgc
     1441 agacacgact tttatttcac ttatccttta tcccataacc taaacttcaa tcattacttg
     1501 tgagctgatc aatgttcttc caagtctgaa agaacattga tcagcttggc aaacaaataa
     1561 tgttttcaaa tacttgatcg atgaaaaaaa gctattggta ataacagtaa ttgcattttc
     1621 cattttcgtg tcagatactt cgcttacagc tggtaaacga tcaatcccat tgtcaaattt
     1681 tggatttttt tctgatgatg tcttgcttta gttcataaaa taatccagca ataaattcct
     1741 cgcaaggata gccaacttca ttttcctaat cggaattttt aaacttatcg gtatagatac
     1801 agatgcaatg agaacaaaca tttatttcga gtcaccatct aaaatatttt cctccatgta
     1861 cccgttcatt attatttagt tatcaccaaa gatatctctg gtataaactt tttcttcaac
     1921 atcctttaaa atatcttcaa accgattaga tacgatcaca tcagaaatat ttttaaattc
     1981 attaatatct ttcaatactt gtgaatgata aaattcatct tcatccaatg taggctcgta
     2041 aacaaccact tcaattcctt tggctttaat ccgtttcata attccttgga tcgacgaata
     2101 tcgaaaatta tctgaaccgg ccttcatcgt taaacgatag attccaacaa catttggttt
     2161 tttcgccaaa atctgtccag caataaaatc tttccttgtt gtatttgcat caacaattgc
     2221 actgatgata ttgcttggta ctttttcata gttggctctt aattgttttg tgtctttggg
     2281 taaacagtat ccaccatagc caaaagaagg attattataa tgcgaaccga tccgtggatc
     2341 caaaccgaca ccttcaataa tttgcttact atcaagccca cgaaattctg catacgtatc
     2401 taattcattg aaatacgcca ctcgcaatgc caaatatgta ttcgagaata atttaatagc
     2461 ttctgcttca gttgagtgag tcaaaagaac tgatgcatct ttgttaaatg cgtttctttg
     2521 aaacatagtt gcaatctcgc gaccacgagc agaacattct ccaactataa ttcttgatgg
     2581 atataagttg tcgtacaaag cttttccttc acgcaaaaat tcaggtgaaa aaatgatgtt
     2641 ttctgtacca aatttcgctt taatttcatt tgcatagcca actggaactg tcgacttaat
     2701 aatgaatgtt gaatctggac gaattgctaa agccttctca attacatctt ctactgttga
     2761 tgtattaaaa tagtttttct tttcgtcata atctgttgga gttgcaataa tcaagtaatc
     2821 accgaagttt actgcatcag taaaatttga cgtaaattcg acatttaagt catcccgttt
     2881 taaaaattca tgaacttctt tatcttctat tggtgagatg ccattttgta acatcgtgat
     2941 tttttcttcc acgatatcat aagctttgac ctgctctttt tgtgctaata atagtgaatt
     3001 tgctagacca acatagccta atccgaaaac tgaaattttc atattcttcc atacgctcca
     3061 atcaaatttc attttatagt attttagctg ttatcctttt gaacatattc cttattcaat
     3121 caatattatt taatagaaaa aatttttttg cttcaaaatc atcgaggttc gatatttaa
//
LOCUS       DAYYAJ010000195         3163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000195 DAYYAJ010000000
VERSION     DAYYAJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.201"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..2556)
                     /locus_tag="ACHATZ_002891"
     CDS             complement(64..2556)
                     /locus_tag="ACHATZ_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8405345.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2563..3030)
                     /locus_tag="ACHATZ_002892"
     CDS             complement(2563..3030)
                     /locus_tag="ACHATZ_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8405346.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1110 t
ORIGIN      
        1 tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac acaggccaag
       61 taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg tgatcttacc
      121 ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa gcaaagcctc
      181 gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg caccatattc
      241 aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt tcacttgaat
      301 atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga ttgtatggat
      361 ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca agaattcagg
      421 tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt gatctttagt
      481 aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg ctccgatatt
      541 tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag aatctgttaa
      601 atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg ctttctccac
      661 ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca actgtccacc
      721 ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac tgtatttttc
      781 cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca tgacttctga
      841 aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca tgaatgaacc
      901 aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac gtgaaactgc
      961 tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca gttctaacaa
     1021 gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact ccgatacaac
     1081 tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt ttgcttcaat
     1141 tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta attgagcagc
     1201 attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt catcgatttc
     1261 tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga ctttcgctgc
     1321 tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga tataacgaac
     1381 agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat ggtgctcttc
     1441 atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg gctcatctac
     1501 ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt tttgatattc
     1561 atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg gtttcaagat
     1621 attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat gcaattcatc
     1681 aatgaataag attacttgac catcattata gatttcgtcg atgacctttt tcattcgatc
     1741 ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa gcatcatcag
     1801 gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag ctaacccttc
     1861 agcaatggct gttttgccta caccaggttc acccaccagt acagggttat tctttgtccg
     1921 tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa ctgggtccag
     1981 cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg taggtgttcc
     2041 ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca ttccttttcc
     2101 agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt ttgaaaggct
     2161 gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta ataagctcat
     2221 caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc tgctagctaa
     2281 agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag ggtaagaacg
     2341 gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt cattcaaatc
     2401 catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg ctagtaaaac
     2461 atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg caatttctaa
     2521 tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc catcttcata
     2581 acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt catattcatt
     2641 gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt tttttgtaat
     2701 tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac tgattgtatc
     2761 atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc ttacttttgc
     2821 aattcgtata taaccaccac caccacgttt gctttctact agataaccac gttggatggt
     2881 aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga ggtctgccat
     2941 ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta tatatgattc
     3001 aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact aatattgacc
     3061 ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta aataaatcct
     3121 ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DAYYAJ010000196         3093 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000196 DAYYAJ010000000
VERSION     DAYYAJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.202"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACHATZ_002893"
     CDS             14..1141
                     /locus_tag="ACHATZ_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8405347.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACHATZ_002894"
     CDS             complement(1359..2501)
                     /locus_tag="ACHATZ_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8405348.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACHATZ_002895"
     CDS             complement(2506..2691)
                     /locus_tag="ACHATZ_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8405349.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT         1029 a          554 c          480 g         1030 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agc
//
LOCUS       DAYYAJ010000197         2843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000197 DAYYAJ010000000
VERSION     DAYYAJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.203"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..416
                     /locus_tag="ACHATZ_002896"
     CDS             159..416
                     /locus_tag="ACHATZ_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405350.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            430..762
                     /locus_tag="ACHATZ_002897"
     CDS             430..762
                     /locus_tag="ACHATZ_002897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405351.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            807..1355
                     /locus_tag="ACHATZ_002898"
     CDS             807..1355
                     /locus_tag="ACHATZ_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8405352.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            1362..1814
                     /locus_tag="ACHATZ_002899"
     CDS             1362..1814
                     /locus_tag="ACHATZ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405353.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            1911..2252
                     /locus_tag="ACHATZ_002900"
     CDS             1911..2252
                     /locus_tag="ACHATZ_002900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405354.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            2620..2811
                     /locus_tag="ACHATZ_002901"
                     /pseudo
     CDS             2620..2811
                     /locus_tag="ACHATZ_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1038 a          305 c          542 g          958 t
ORIGIN      
        1 agaaatagaa aaaaacagac tgttttttta agaaaaacta tgtcattctt tacttgaaaa
       61 aagttgttat aggacagaaa ggataaaaca aaaacggaaa aaatatgtct tatgaataac
      121 tataagaact attctatttt ttaggagatg gatacgagat gaaaaaacta attggctata
      181 gtgtgatgtg tattggattt gttggtgtgg gattgtttgt atcgactgaa tctgcttctg
      241 cggctgaatg gtataatcct atctggtata atccgaacat tgaatgtcaa aaaggctatc
      301 cgtatggacc gacaaatctg aaaaatggaa aatgtagagt aaattggaat ggcgttgtag
      361 atagtgtaat tactggttca atggactctg ctctacatgg ctttacgggt cactagaagg
      421 aggaagaaaa tgtctgaaga gaaaaaagaa atcgttgaat cattagtagc tttaaaagat
      481 tctctttcta agattgaata tgttgatagg caagaaattc aaaaactaat tgatgatact
      541 attattgaaa tccaagatgc gcgttgtgaa ggtataaaaa tttctgtagc tttatctaaa
      601 gtaatagaaa aaatgaaccg ttcattagcg tttaatggat tgaagctaga tagacaaact
      661 agtctgatat gggatcattt aaaagacttg tatgacaagt ctaaaagaag tgaacggact
      721 gctgtatcaa tcttaaaagg cctttgggga atgaattcat aaacaagata tttaaaaagt
      781 aacttatttt ttatggagga gaaattatga aaagaataat taaaatattt ggtgttttag
      841 gtgttttctt attaggttta agtacttttg ttagtactgg aacaagtgtg tatgccacag
      901 aaaaagtgaa tacagttgat ttagaaataa agaataaatt agtagatgaa ttaacttttt
      961 attttgatac agttggtttt ttagacgaac atggtgtata ccatataaag gattttggtg
     1021 ctattcgtga acgaatcagt caagataacg aacaaggaag agcagcaaga gtcttttatg
     1081 aacatattat tgttcctaga atggagaaag ttgattggaa acgatatgct ggatgtgtag
     1141 ccattaattc tcttcctgga ggaggagtgg cttgggctat aggtacgcag ggagatttta
     1201 ttgaagcatt aaaagctttt aattttgaaa aagctgcgga tttattatta aaaattgcag
     1261 aaaaacaatt aggaaaagct gcagctaaag agttagctaa ggtaaatatt gtagcaggaa
     1321 ttgcagcagc tgctgctagc tgtgcattag agtaaaagtt tatgaaaagt ttagaacaag
     1381 agcatatggc ttttaaacaa gcaatgttta aagaaaatat ctatttaaac cataactata
     1441 ttcgtgtttc taaggcatgt tcaccagtat tgaatatgct tggaggtgga aatggattat
     1501 atcatttact gtttgtcgat gtttgttggt taattttttt accagatgaa ctagttatag
     1561 tcaatgaaaa aataacaagt aaaaatgaag tgtttaatta ttctttaaca agaattaatt
     1621 acaaagagat taccaagttt tcactagaaa aagttccttt ttggggagaa ttttgtttaa
     1681 aaattaaatg taactggaaa agaatgtatt tctatattga tggtgatgat gctcttacat
     1741 tcggtaaaac tactttttct agttttaatt ttcagtttct attaaaaaat aatttttatg
     1801 gtttattgaa ataaggttgc gaaggaagat gtagagttat ttgcactgtt gtgttggtat
     1861 atctcttaat gctgtgtctt tttggggaga ttaattagtg gagtgattat atgagtatag
     1921 atgattgttt agaagaacaa ataagattat ttttaaagtt tttaaaagtt aaagttcaaa
     1981 ataactactt cacatgttat agttatgcac accctgtttt aaatagtctt ccttttaatt
     2041 ctttagtgca aggattgatt acaaatattg ttttattagt ctgtacacca aatgaaatat
     2101 ttgtaaaaaa gataggcagt ggtatttctt cagtaagatt aaaagagaaa gaatatactc
     2161 aaaagattat tcatattcct aagcctgaaa ttaagcgttt tgagataaaa gactattctg
     2221 ctttagggct gttctcaatt ggttactggt gataaaaaat aagaatacta aaactatacg
     2281 tagaatataa gtaatcggca tcaatcctaa ttatgtgatt gaatgccgat ttttgttacg
     2341 cgctattttt aattatctgt aagaagaata attttatttt actcatattc gttttcactt
     2401 tgaagaagta aattgcaatt tcttataagt taagcagcta tgcaaaaggc tatttattct
     2461 attgtgaaat ataagatgta gttgaaacag gatatataga atcaaaatac gaatcttatt
     2521 ttgatttttc tagttttttg aaaaaagcac cctttgatcg ttgtattagt ggaaaagaga
     2581 aaaaggagca tacttatcat gtcacaaaac tacaactaaa tatctaaaca acaccaattt
     2641 gatgcctatt gaaaaaaagt attgcgaaat gaagccaagt ctattagaaa atgaaataat
     2701 agaataaaag aaaatgggaa accgctgaat gatgtaaatg aaggtaagta tccacgaact
     2761 aattttgatg aacaagatat ctatttttta tttgtattgg agatactagt ttaatatcaa
     2821 aagttatcag cagtcataat tag
//
LOCUS       DAYYAJ010000198         2679 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000198 DAYYAJ010000000
VERSION     DAYYAJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.204"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2358
                     /locus_tag="ACHATZ_002902"
     CDS             <1..2358
                     /locus_tag="ACHATZ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8405355.1"
                     /translation="GFKKFIYNKYKNEIKFTNINYLTSKYISNFEELIDKLDTKDIVN
                     WLNQDEELTDLLSSVKKESSASQQSNWSGLYFNSNEYVKYILNKTAWIKIDGEKYSPR
                     QIVLYEKLQAHVPGLYGVSEQSLMELLGQRIVEQLDFRKNMAMFSDDEIRAILVELPK
                     FDKGEISRRLYNELIRNKKGMNPTYSTEGLSVLAKDGFFYPNKELRYADKRLPKSIEK
                     QMRFIYVPEKASTTTIYEWLGVERFKTNLKLETFELLEDYSEDFKKEIDDIKTAVLST
                     IDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQSIVLEDYYFVESKGKFYLKLPANSIE
                     INQLRLIDTFSSTIVDIFKQVLNLSLDLNLIELLISRDTEHKQRKITEEFGVDKWNES
                     FELLFSENALNKKVNDFLLSNGLNEEEYPTIFEIDYSYALKEEEYQLLAIALESISKD
                     IEDLNGLSELIHLDIREFWKLKLRSYLDKKAEHYRQILYSNANKSGDEILKETFLEKI
                     DRYKDYQFEISEIPNSVQVDIEKESKKRFPELSVSITDEIDIDGLYDNNVEELVTVAA
                     ITIDDFDYYIRGHQKVSSLLYFGIPSRLREDILIFVTKENDVDLNGEKSGISIEGTST
                     VQTILEPSNSVSRKGKNTRNGERSKRDYDNRNSANDDAGETAEQIAYAELTQNQKLNV
                     IWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGNMYFEVKSSITEFEMSINEYNSMLD
                     NPDSYEVVLVNRDTKEISRHKFDELDGLKQASSYLFKFKQKRSDG"
     gene            2493..2621
                     /locus_tag="ACHATZ_002903"
     CDS             2493..2621
                     /locus_tag="ACHATZ_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8405356.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         1032 a          345 c          505 g          797 t
ORIGIN      
        1 ggatttaaaa agtttattta taacaaatat aagaatgaaa taaaatttac gaatattaat
       61 tatttaactt ctaagtacat cagtaatttt gaagaattaa tagacaaatt agatacgaaa
      121 gacattgtca attggctcaa tcaagacgaa gaattgacag atttattatc ttcggtaaaa
      181 aaagaatcaa gtgcaagtca gcaatcgaac tggagtggat tgtactttaa ttcaaatgag
      241 tatgtgaaat acattttgaa taaaactgca tggattaaga ttgatggaga aaaatatagc
      301 ccgagacaaa ttgtgctata tgaaaagctc caggcacacg ttccgggatt gtatggagtt
      361 tctgaacaaa gcttaatgga actcttgggg caacgcattg ttgaacagtt ggatttcaga
      421 aaaaatatgg cgatgttttc ggatgatgaa ataagagcta tattagttga gttaccaaaa
      481 ttcgataaag gcgaaataag tcgcagatta tataatgaac ttataagaaa taaaaaagga
      541 atgaatccta cttattctac cgaaggatta tcagtcttag ccaaagatgg cttcttttat
      601 ccaaacaaag aattaaggta tgctgataag cgactgccta aatcaataga aaaacaaatg
      661 cgttttattt acgtgcctga aaaagctagt acaactacga tatatgaatg gcttggggtt
      721 gaacgcttca aaacaaattt aaaattggaa acatttgagt tattggaaga ttattctgag
      781 gatttcaaaa aagagattga tgatatcaaa actgcggtat tatctacgat agatgacaat
      841 agtaagaatg ttagttcatt aaaacgtatt cagattattc catgcagcaa aatatctgct
      901 aaagatgttg agcaagatgg tcagtcaatc gttttggaag attattattt tgtggaaagt
      961 aaaggaaaat tctacttaaa attacctgct aattctattg aaattaatca attacgtttg
     1021 attgatacgt tttcttcaac aatcgtagac atattcaagc aggtgttaaa tctaagctta
     1081 gatttgaatt tgattgaatt gttaatttcc cgagatacgg aacataaaca aagaaaaata
     1141 actgaagaat tcggtgtaga taagtggaat gaatcgtttg agctattgtt tagcgaaaat
     1201 gcgttaaata aaaaagttaa tgactttttg ttatcaaatg gactaaatga agaggaatat
     1261 cctaccattt ttgaaattga ctattcatat gcattaaaag aagaagagta tcaattgttg
     1321 gcgatagctc tagaaagcat ttcaaaagat attgaggatt tgaatggatt gtcagaattg
     1381 attcatttgg atattcgtga attttggaaa ctgaaattaa gatcttatct agataagaaa
     1441 gcggaacatt accgccagat tttatatagc aatgcaaata aaagcggtga tgaaatactc
     1501 aaagaaacat tcctagaaaa aattgataga tacaaagatt atcaatttga gatttctgaa
     1561 attccaaatt cagttcaagt cgacattgag aaagaaagta agaagagatt tccagagcta
     1621 agtgtgtcaa ttactgatga aattgacatt gatggcctgt atgataataa tgtcgaagag
     1681 ttagtcacgg ttgcagctat tacaatagat gattttgatt attatattag aggtcatcaa
     1741 aaagtttctt ccttactgta ttttggtatt ccgtcccgtt taagagaaga tatactgata
     1801 ttcgttacta aagaaaatga tgttgatttg aacggagaaa aatctggtat ctctattgaa
     1861 ggaacatcaa ctgttcaaac cattcttgaa ccatcgaata gtgttagtag gaaaggtaag
     1921 aacacacgaa atggagaaag aagtaaacga gattatgata atagaaactc cgcaaatgat
     1981 gatgctggtg aaacagcaga acaaattgct tacgctgaat tgactcagaa tcaaaaactt
     2041 aatgtaattt ggaatacgaa atattctagt aaatcttcag atcgtaataa gcagccacca
     2101 aacggaattg tttgtgatat gtgggttcat gacccagaga atgggaatat gtactttgag
     2161 gtaaaatcat caattacaga atttgaaatg tcgattaatg aatataattc tatgttagat
     2221 aaccctgact cttacgaggt cgttttagtc aatcgtgata ccaaagagat aagtcgtcat
     2281 aaatttgatg aacttgatgg attgaaacag gcaagtagct atcttttcaa atttaagcaa
     2341 aagagaagtg acggttgagt gatttgtttc cacaaaccta ttcaatctat caactcaaaa
     2401 atcataaaca accattatta tagtaggaag atacagagtg tactctcaac gcatatcgaa
     2461 ttagtcgctt cattcgttaa aggagaataa aaatggacca aaaagaaaaa atgaaagaat
     2521 tgatcaaaaa gaaacaaggt ggaggccgat ccaaacaaat ggaaacacct aagaatgatc
     2581 gtaaaaacat gagaaaaggc cctaaaatct ttaataaata gacgtgctaa agaaagacta
     2641 agaagtgtta acttttgtta ttgtttctta gtcttttta
//
LOCUS       DAYYAJ010000199         2675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000199 DAYYAJ010000000
VERSION     DAYYAJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.205"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACHATZ_002904"
     CDS             699..785
                     /locus_tag="ACHATZ_002904"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8405357.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACHATZ_002905"
     CDS             complement(1087..1311)
                     /locus_tag="ACHATZ_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405358.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACHATZ_002906"
     CDS             1677..1961
                     /locus_tag="ACHATZ_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405359.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACHATZ_002907"
     CDS             1964..2380
                     /locus_tag="ACHATZ_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405360.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACHATZ_002908"
     CDS             complement(2517..>2675)
                     /locus_tag="ACHATZ_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8405361.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAYYAJ010000200         2674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000200 DAYYAJ010000000
VERSION     DAYYAJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.206"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..914
                     /gene="truA"
                     /locus_tag="ACHATZ_002909"
     CDS             174..914
                     /gene="truA"
                     /locus_tag="ACHATZ_002909"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8405362.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1059..2672)
                     /locus_tag="ACHATZ_002910"
     CDS             complement(1059..2672)
                     /locus_tag="ACHATZ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405363.1"
                     /translation="MDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNV
                     WSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVS
                     YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYI
                     RDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVL
                     TFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLG
                     LIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         1012 a          388 c          485 g          789 t
ORIGIN      
        1 tgaacagtca gcggatactt catccggtgg ctgttttttg tttttctgaa atatatattt
       61 ccacagtttt cttgtatagc ttctgtctta gagaagcaag gagctatatt ttgtgctaca
      121 atcttaaaaa ggtagagaga agaccaaatg gaagacagag gagccactaa aatatgagaa
      181 acatcaagtt gacaatcgaa tatgacggaa aaagatattc aggatggcaa agacttggag
      241 atgacgataa aaccattcaa ggaaaaatcg aaaaagtatt gcatcagatg acaaatgaag
      301 agatagaaat tattggatca ggaagaacag atgctggcac ccatgcgcga ggacaagtag
      361 caaattttaa aacaaacaca gaaattggac tggcagaaat gatcgacttt atgaatcgtt
      421 accttccgcg ggatattgtc atcaaaagaa tcgaagaaat gcctgagcgc ttccattcaa
      481 gatataatgc ggtgggcaaa aaatacagct attatgtttg gaacaacgtg attccttcag
      541 catttgagcg aaaccacagc ttttatttcc ctcaagagtt ggatatggac aagctgaatg
      601 cagcttgcga aaaattagtg ggtacacatg atttcattgg tttttctgca ttgaaaaaat
      661 caaaaaaatc cacagtgcgt acgattgatg aattatctat cgaacgaaag ggagaaatgc
      721 ttcattttac ttttgtcgga gacggattct tatataaaat ggtgcggatt atcatgggga
      781 cattactaga aatcggcaca ggtgccatgc cgatagaaac aatcgatgaa atatttgaaa
      841 gcaaagtgcg gcaacatgca ggagaaaccg tccctgcaca aggtctgttt ttggatgaag
      901 tttattatcg ataaaaatac tttgataatg gatgaaacgc atgtttcaca gtataaatat
      961 cgatgttaaa taatgtatga agcaaattaa agaaatataa aaaaagactt tttagcctgt
     1021 ttatgtgtac agctaaaaag tctttcttaa ataaaaatct agtgagaatg ttgtacttct
     1081 cttctcggtt gtttttgttt agaaaaagta aatcgataaa gtaaaaataa aatcaaagta
     1141 acgatagata aatatcttga agcaattgct agttttgtgt actgataagt aataacgact
     1201 ttatttatac ctggaggaat agttaattca gttgtgccaa acttagataa ttttgtttgt
     1261 actagtttgc cattaataga agccacttgc cctttataac gataaatagg tgtcgtcaag
     1321 acagtatttt tattattcga attatctaat tcaattgtaa gtttgttact cataaaatga
     1381 gttgttggat ttatttcctg gttacctagt aaatattgat cattattaat catatcagga
     1441 tgatagattg actctttgtt tgcataatca gtatgattgt aattttcagc tatagcaaca
     1501 gcgtcttcag gtgctataag tgttaatgaa tctttgttag catggataga attgcttact
     1561 cctgagagat gcaatattag tgcaagaaac gtaaagacca ttattttcca tgaattattt
     1621 ttagtagaaa aaatataata gatcactata ctcatcgtat aggctataaa taaagaagaa
     1681 aaaccattta accgccagac aaattgtaga ctcttaactg gtgtatggtt aaaaagttgc
     1741 caaggaaata aattggttga acaaaaaaga acaaatacac caagaaagaa aataaataaa
     1801 tcatcagcag ttaatttttt tataaaaata aaagcaccaa tcaatccgag aaataagaat
     1861 aatccaatag tatagttgtt atagttatta tttaaaatat gtgtcagcat atcactagga
     1921 gccataccac ttaattcttt tcccagtggg actttcaatt cttgtgctct agattgttga
     1981 ataaaaggaa ttaagaatcc cgcacttaaa aaaaaggtga ggacggtagc ttttaaaaga
     2041 cttaagagtc tagcgatttt ttggtcccaa aaataaaaag aaagaaacaa cgttcctcca
     2101 ataaatacag aaaccatagc gacagaaagt aaatgagtat acactactag cgtcatgcca
     2161 atagacaacc agtaccattt ttggtaatct ctaatataaa tttcgtaaca ccccattagt
     2221 atcaaaggca aaaatgttaa agcgacagct tctcccaatg aagcacgatg gaatatgtca
     2281 atggcacgat aggcggaaaa tgtataaata atcgaaaata acagcgaaat ataacgattg
     2341 ttttttattt gtttcataga aaaataagaa acgaccattg ttaaaaaagt gataaaaaaa
     2401 tagtagatat tataacttaa aacaagattt cccatcattt tataaaataa aaaagctgga
     2461 taaagagtaa gccatggata aaaaatattc atcattgttc catgatgatc gaagttattg
     2521 aagtttactg gagaactcca tacattatct agtcctatga ttcgagataa atgaaagtaa
     2581 gtatcctgat tgacactatg atagattccc ttagacatga atagagggat gacgtaagca
     2641 aaacttagaa ataacataaa aatgatatct atta
//
LOCUS       DAYYAJ010000201         2609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000201 DAYYAJ010000000
VERSION     DAYYAJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.207"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            314..634
                     /locus_tag="ACHATZ_002911"
     CDS             314..634
                     /locus_tag="ACHATZ_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8405364.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            795..1700
                     /locus_tag="ACHATZ_002912"
     CDS             795..1700
                     /locus_tag="ACHATZ_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8405365.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1697..2470
                     /locus_tag="ACHATZ_002913"
     CDS             1697..2470
                     /locus_tag="ACHATZ_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8405366.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
BASE COUNT          811 a          463 c          497 g          838 t
ORIGIN      
        1 aatttattgc ctgctaatat ataaaaataa ggtctagtta ctgttttttt aaatgattaa
       61 taaataacaa ctcctttcta tgaaattgca aagcattcat cttcttttct gctaacgata
      121 tatccgtcaa tgcttgttac actttactaa aaagtatgag attgccgata tctggatagg
      181 tgtgctatac tacagaaaaa gatatacgat ctgccaccgg gtagtaaaaa agaggatgat
      241 cattccatta ggtctttgaa gggatgattc tcctcttttt gtgtattttt tatattgaag
      301 gaggaacgta tgtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
      361 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
      421 gattggaatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgcctatgag
      481 tctctcttat cctatttgga cagggattgg cgcagttggt tcggtcatta ttggtgtggt
      541 cttgtttcat gataagctga atctttccac ttggttcttt gtcgggttgc tgctcatcag
      601 catcattggc atcaaaatca ctagcggtca ctagatactt agacaagcag acaaggactg
      661 atcagaaaaa gcggggcttt ttatttgata ttcctctttc tatgaaaaaa caagaaaata
      721 agactaatga cctagcgact tctctactca tcaggtgtat gcttgtacta tctatagaaa
      781 aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac tttggtcaca
      841 aacaagccct cagcgatgtc agttttagct tatctcctgg caaaatcgtt ggattagtag
      901 gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg atccattact
      961 cagaaggaac gatccgtatt ggtgaaaatg agatttctcc ctctagtcat aaaggattag
     1021 aacaagtagg agcattgatc gaatatccgg gtatctatcc attcttaaca ggttatgatc
     1081 atctgaagtt attctctgaa acaaacgatg tatctgctat cgacactatc gtgaaacagc
     1141 tcaagatgga aaaatacatt cacaaaaaag cgaaatccta ctcgctggga atgaaacaaa
     1201 aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta gatgagccga
     1261 tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc gacttggcta
     1321 ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa aaaatggtcg
     1381 atgagctgat cctgatcaat caaggaaaaa tcatcaaaca atgtacaatg gctgaattga
     1441 accaaacagc gcaagatttc atcgttatct ctacgacaaa tgatcaagct gctcgaacga
     1501 tcttgacaga cgctggatat cccttggaag atcctgataa aatcaagctt ctaaaaaaaa
     1561 acgaagagtt attaggaact gttatccagt tattgtctgc agaaaatgtc catgtgacag
     1621 atgtccagca tctgacgaat gatttggaat cttccctatt ggaaattctg gattcagtaa
     1681 agggggaaga agaaaaatga cgttgcttat aaaacaagaa ttatttaaat tgatcaagaa
     1741 aaagtcaact gctgttttat ctgttctatt agttgctttg ctcataggaa cagcacttct
     1801 agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc ttttcagtgc
     1861 cacatcttgg attgtcttta tcatgattgc tgctgcaagt acgatcattt caatggaatc
     1921 acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag aaatattagt
     1981 aagtaaatgg atcacattag ttatttattc tatttattta taccttttag ctattattgt
     2041 caccgtgctg atgaaactga tccttttccc ttcgatttcc tttacggaga agatttctac
     2101 tggtcagaca ctgatccaaa gcttattcac ttatactttg ggtagttata tcggtttatg
     2161 gctaatttta agtttagtgt tgatgattgc ttgttttatc aatagttccg gtgcctccat
     2221 ctcagcaggg atcgtctttt attttgcttc atcaatcata tctggtatcc tcatcgcact
     2281 cattcaaaaa tgggagtgga tcaaatggaa tccaatcagt atgctgaatc tgcaaaacca
     2341 aattggcaac gaagagatta tgaagcaatt gactcatctt tctactaatc aaatgctgtt
     2401 tggcaattta gcttacattg ctttgttctt agcattggga tacctcgtat tcaagcgaaa
     2461 aaatgtatga tggttcatta acaaagaagt tgtgacagaa gtggccagtt tcaagaaatt
     2521 tcggcttatt tttagaagaa gctacttctt gaacaccatt tattcaaaaa aagaagcgat
     2581 gacacgaatg ttgtcaccgc ttctttttt
//
LOCUS       DAYYAJ010000202         2399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000202 DAYYAJ010000000
VERSION     DAYYAJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.209"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHATZ_002914"
     CDS             207..2069
                     /locus_tag="ACHATZ_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8405367.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="ACHATZ_002915"
     CDS             2069..>2399
                     /locus_tag="ACHATZ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8405368.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DAYYAJ010000203         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000203 DAYYAJ010000000
VERSION     DAYYAJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.210"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHATZ_002916"
     CDS             316..570
                     /locus_tag="ACHATZ_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8405369.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHATZ_002917"
     CDS             571..888
                     /locus_tag="ACHATZ_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8405370.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHATZ_002918"
     CDS             1447..2382
                     /locus_tag="ACHATZ_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405371.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYAJ010000204         2308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000204 DAYYAJ010000000
VERSION     DAYYAJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.211"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2158
                     /locus_tag="ACHATZ_002919"
     CDS             5..2158
                     /locus_tag="ACHATZ_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8405372.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          791 a          413 c          513 g          591 t
ORIGIN      
        1 ggaaatgatg aaagatttta tgaaaaaaat attaaatact tcttggttag aaaaaataag
       61 aaaaggtgca caagaaccta agaacaaaac gtttcgccgg tcccatgttc cttttcgctt
      121 aaattttctt tttttcatta tatttacgtt atttgttgcg ttgattgcta gattgggtta
      181 tttgcagatc gtgaacggcg aagagatgga ggcgcgagtg aagtctactt ctacgattac
      241 ggtccaagaa agtactcctc gcggaatgat ctacgactcc acaggaaaag ctttagttac
      301 gaataaagcg aatcaggcca tcacatatac gagaggaaca caagttacgg ctaaagattt
      361 gcttgaaatc gcaacgaagc tgaactcgct gatcgatgtt ccggtagatg aaaatctgac
      421 agatagagat aaaaaagatt tttggctggc tgatcccaaa catttgaaag aagccacaaa
      481 aagactttcg gcaaaagaaa agcaattagg gaacagcgaa caatatagtg cgatggtcaa
      541 taaagtcaca gctgatgaga ttaatttaaa cgaggagcaa ttaaaaattg ctaccatctt
      601 taaacggatg aatagtgcat atgaattaaa cactgtctat atcaaaaact ctggtgttac
      661 tgatcaagag ttagctgttg tggcagaacg cgcttctgaa cttccaggag tttcgactgg
      721 cacagactgg acacgagaat ataatgcagc aagttcactg aaaagtattc ttggcagtgt
      781 gacgactgaa aagcaaggac ttccagcaga tgaagcagag aaatacctag caaaaggtta
      841 ttctcggaat gaccgtgtag gacaaagtta tttggaaaaa caatatgaag atgtcctgca
      901 aggaaccaaa acccaatatg aagtttccct tgataatgaa ggaaatgttt cgaatcaaaa
      961 agaaatcttc tctggggaaa aaggttctaa tttaatgtta tctatgaatg cagaattcca
     1021 atcaaaagta gaagaaattt tgaaaaggaa ttatcaaaca ttgatcaaca acggcaaagc
     1081 acagtattcg cctggtgctt atgcagtggc tatgaatcct cagacaggag aagtgctagc
     1141 aatgactggt ttcagtcatg aacaaggttc caaagaaatc accgagaatg ctttaggcac
     1201 gattactagc gcctttgatc cagggtctgt agtcaaggct ggtactttga cagcaggctg
     1261 ggcaagtaac gcgatcagcg gaaatcaagt tttgattgat gagccgattc gtctgcaagg
     1321 tgcttcagaa aaaagctctg tctttaatcg aagcggacaa gttgctcttg atgcagtcaa
     1381 agcatcggaa ttgtcttcta atacgtatat gatcaaagtg gcgttaaaga tgttaggact
     1441 agactataca cctggaatgg gactacctag tttagatgaa gaagctaaag cttatcagca
     1501 attgagagac tctttcaaag agtttggtct tggtacaacg actgggatcg atttgccgaa
     1561 tgaatctcct gggatctcac gttctgttga ttatatgaag aagttcaatg cggataatgg
     1621 caaagaatgg tatgctccag gtaactttac cgatttggca tttggacagt ttgacacata
     1681 tacaccaatc caattagctc aatatgcttc aacagtcgct aatggcggga aacgagtaca
     1741 gccgcaccta gtcaaagcga tctatggcaa tgacgaaaat ggaaacttgg gtgaagtgaa
     1801 gaaagaaatt ggcacaacag tagaaaatac ggtcaatatt tctgcagaaa atatggcgat
     1861 cctccgtgaa ggattccacc aagttgtcca tggtaccgat ccttacacaa cagccaaacc
     1921 attagccagt gcgaaaatgg acctttccgc aaaaacaggg actgcagaaa cggttgcgga
     1981 aggtcatcca gatattgcga cagtcaatag taatatcgtg gcatacggac caacagacaa
     2041 tccggaaatt gccatcagtg ttgtcttgcc gaacttatta gacgaacaag accacataaa
     2101 cttgacgatt gctaaagaaa taatggacgc gtattatgat atgttcatgg caaaataaaa
     2161 aagaggtcgt gaaaaagccg cttagtttcg agtatgaaga aaaattttgt gaaaatagct
     2221 tctcacattt ttgcttaaat ttgacttcat accgagaaac tggcttttga acaccgttta
     2281 ctcggcaaaa aaggttgtga caaaaatt
//
LOCUS       DAYYAJ010000205         2293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000205 DAYYAJ010000000
VERSION     DAYYAJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.212"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACHATZ_002920"
     CDS             complement(51..1598)
                     /locus_tag="ACHATZ_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8405373.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHATZ_002921"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHATZ_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8405374.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACHATZ_002922"
     CDS             complement(2043..2237)
                     /locus_tag="ACHATZ_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405375.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DAYYAJ010000206         2177 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000206 DAYYAJ010000000
VERSION     DAYYAJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.213"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..587
                     /gene="ahpC"
                     /locus_tag="ACHATZ_002923"
     CDS             24..587
                     /gene="ahpC"
                     /locus_tag="ACHATZ_002923"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8405376.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            597..2126
                     /gene="ahpF"
                     /locus_tag="ACHATZ_002924"
     CDS             597..2126
                     /gene="ahpF"
                     /locus_tag="ACHATZ_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8405377.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT          704 a          371 c          472 g          630 t
ORIGIN      
        1 taattattat tggaggtttt actatgtcat tgattgggaa aaaaatcgaa gaatttaatg
       61 cacatgctta tcatcaagga gaatttttag aagtcagtga aaaagatctt atggggaact
      121 ggagtattct ttgcttctat ccagctgatt ttacttttgt ttgtcctact gaattagaag
      181 acttacaaga acaatacagt acattgaaat ctttaggtgt tgaagtcttt tcttgctcta
      241 ccgataccca ctttactcac aaagcatggc atgatacatc agatgctatc ggtaaaatcg
      301 aatacgtgat gatcggtgat ccgtctcatc agatttctcg tatttttgat gttttggatg
      361 aagagcaagg attagctcag cgaggaacat ttattattga tccagacgga atcgttcaag
      421 ctatggaaat caatgcagat ggaatcggtc gtgacgctag tgcactgatt gataagattc
      481 gcgctgctca atatgtacgt acccacccag gtgaagtttg tccggcaaaa tggaaagaaa
      541 gtggcgaaac attgaaacca agttttgatc ttgttggaaa gatttaggtg gtggatatgt
      601 tagacaaaga aactaaacaa aatctcgaac aatatttagc actgattgaa tctcctattg
      661 ttttttctgt tagtttggat aatagtgaaa atagtcaaaa actagctgaa tttacgaagg
      721 aaatagcaga aatgtctcct aaaatctctt gggaaaaaag agattcgatg cgcacaccta
      781 gtttttctat caatccagtt ggcaaagaaa gtggaatcgt ttttgctggt atccccctcg
      841 gtcatgaatt ttcctctttt ttactggcta tgttacaaat tagtggacgg gcaccgaaaa
      901 tcagtgaatc tttgagtctt aaaatcaaaa aaataacaca aacattgcat tttgacagtt
      961 atgttagctt gacttgtcat aattgccccg acgttgtaca ggcattgaat atattagctg
     1021 tattaaatcc aaatattact catacgatga ttgaaggtgg gatgttccaa aaagaaatcg
     1081 atgataaaaa aatcatggca gtgccaactg tgtttttaaa tggagaagaa tttagtagcg
     1141 gacgcatgac gatcgaacaa attgttgaaa aaatcactgg ccctttgatt gaagaagagt
     1201 tgtttgaaaa agaaccttat gatgtattag tgattggcgg tggaccagca gctagtagtg
     1261 cagctatcta tgcagcacgt aaaggaatcc gtacaggact tgtcgctgat tctttcggcg
     1321 gacaagtcgt tgaaacacta gggattgaaa atatgattgg tacctcttac acagaaggtc
     1381 caaaattgat gcagcaagta gaaagtcatg tgcgtagtta ccctatcgac attatgatga
     1441 atcaacaggc cgtttcctta aataaagagc aacattttat taatattggc ttagctaacg
     1501 gtatatcact taaagcaaaa accgccatca tcgcgacagg tgcgcactgg cgttctatca
     1561 atgtaccagg cgaaaaagag tttaaaaata aaggaattgc ttattgccca cactgtgacg
     1621 gccctttatt taaaggaaaa gagattgctg ttatcggtgg tggtaattct ggtgttgaag
     1681 cagcaatcga tttggctggg ttagcaaaac atgtatatgt tctagaattc ttaccagaat
     1741 taaaagcaga tcaaatctta caggataaac tagtctctct tgcgaatgtc actgttatca
     1801 aacatgcagc gacgaaagaa atcagcggta cgaatcaggt agaatctctc tcctatatcg
     1861 atcgccgtac gatggagatg catacactag ctgtttcagg agtattcatt ctagtcggct
     1921 tactccctaa tacggattgg ctagatggaa cgatcgaaat gaatccaaga aatgaaatag
     1981 tgaccgagaa aaatggcgca acgaatatcc ccggcgtttt cgcagctggt gattgtacag
     2041 atagtccata caaacaaatc attgtttcaa tgggttcagg agctactgca gctttaggag
     2101 cattcgatta tctgatgcgc acttgagcta tgaagattgt aaaaagtcag agagcggaaa
     2161 aaaggatggt gacaaga
//
LOCUS       DAYYAJ010000207         2143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000207 DAYYAJ010000000
VERSION     DAYYAJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.214"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            756..1088
                     /gene="traE"
                     /locus_tag="ACHATZ_002925"
     CDS             756..1088
                     /gene="traE"
                     /locus_tag="ACHATZ_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8405378.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1707..2048
                     /locus_tag="ACHATZ_002926"
     CDS             1707..2048
                     /locus_tag="ACHATZ_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8405379.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          758 a          281 c          439 g          665 t
ORIGIN      
        1 attatattac gaatattaca aaataatagc tattttaata gaatttataa gttaaaatat
       61 tatgataaaa atcacaaacg tagcctttta cgtcataagt gttaaaagaa ataattaatt
      121 tcaaatatag aaaagggaac gtatgtttgc atactccttt tttttgagtt ataataatac
      181 gcgaagagag ataatttcat tatttaaaca agctaaaaat cttgatatta cttcttttca
      241 ggcaatacga atattattcg cttacatttt ttgtataaat ttaattgtct gctatgcttg
      301 tgatgaattg attttcgttt tgcacttttg gatgataaat ttggacaatt gaaaatacaa
      361 aaaaaagaaa ggcggtatga actcggaagg ctggtaacct tcgtcagcgt tcatacacgg
      421 cattaagatt tggcccttaa tgcgttaacc tttcagtgtt acggtattac ccgcaatgga
      481 tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta aggttaaaaa
      541 tacgtgatat tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct
      601 ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttggatgc gttccctctt
      661 ttttgtttgt catgaacgaa aatcaattct ctatttttga aaaataacaa gaaaaacttg
      721 ttaaaaatat ttaaagaaat tgggtggttt attttatgtc atatacagga attttgagtc
      781 tcaaagatat ttgtcactac ggtaaacggt gtaccgctac agagaaaatc acgaagaaac
      841 tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa atatataatc cagaaagaca
      901 aagtctcaga ggaaatgatt tattacattg ggaaacaaaa gcaaataatt ttaaaagatc
      961 ctatcccctt aaaagagcta tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac
     1021 tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg gggcgtttga
     1081 tcggctgaat tgatcattct cataacttgt attttatcgt ttttcgcttg cttgaaaata
     1141 ttgtcgagac ttctaaagga acttagagtc actagaaaaa taaggagtag acttgatcta
     1201 tcaacaattc ccttcgggga gtgacggggt aggttttaac gattggtaac gtaaaaaatt
     1261 ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa tgaatttgtg taaataataa
     1321 gcgcagagta aacaagaggg aatttagaag ttagacaagt tagaatagcg tctagattaa
     1381 tcatgagcag ctactaaggg atctagactt tgacgcaaac tcatggaata aggagtaggt
     1441 cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata
     1501 cttgcgagga cgttgattat tggatcaatc gagcatttac cgctgtctac tagggtttct
     1561 cgtttcggac gccataaacg agtcgaaatt ttttatgtat ttttaagtaa acgaaaaacg
     1621 ataaaatatg aacaagtcct gaataaagtg aaaaaattga tataaaacat agagaaaatg
     1681 aaatttacgt aatggaggaa tgacaaatga aaaattatga tccgaatata cgattgggca
     1741 cacatactat taaggtttct ttccaacgtt gggattacaa aggatttgtg acgtttagaa
     1801 ggggcggaaa ttgtaaagga ttagatgttt tagcattaga cgaggatgat ttatacgatc
     1861 aaacattgac tgataatcca attggattcg gcttgctacc tgaagatgat gaaggtaatg
     1921 aatggtttaa aatgacgctt atgaatgata atggagacga attatctgtt gaagatactt
     1981 ggtcatattt gagtgattat attgtcagct ttgaaatcat tgaatttgta gcggataaag
     2041 aagaataaaa actggaggaa taaattttac agataaaggc ttagaaggag tcacgacgat
     2101 taataaaaag gtagtattac cttaggcatt gagtaaggaa ttt
//
LOCUS       DAYYAJ010000208         2072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000208 DAYYAJ010000000
VERSION     DAYYAJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.216"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACHATZ_002927"
     CDS             complement(<1..103)
                     /locus_tag="ACHATZ_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8405380.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITT"
     gene            471..827
                     /locus_tag="ACHATZ_002928"
     CDS             471..827
                     /locus_tag="ACHATZ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8405381.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(1068..2072)
                     /locus_tag="ACHATZ_002929"
     CDS             complement(1068..2072)
                     /locus_tag="ACHATZ_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8405382.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          630 a          393 c          338 g          711 t
ORIGIN      
        1 ctgtagtgat cgcagcagtt aaagtagttt tcccatggtc aacgtgtcca atcgtaccaa
       61 tgttaacgtg tggtttactt ctgtcataat gttcttttgc cataatttac aaacctccta
      121 aataatatag atcactgaaa aatttcagct ctcacctttt tattatagtc tttttttgac
      181 taaatcaaaa agaatacgtc cgacttactt tttgtaagat tcgaactaag cttattatac
      241 gtcaatattg gtcaaagtca actaagagca ctcgtctgtc ttatacgcga aaaaagtacc
      301 ttttataaat aaaaaaatgt gttttttgag aaaaacttca agatagtcct taactttctt
      361 ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc agctgttgcc aacaactttc
      421 aatagacgaa atcttctatt tcgttaataa taaggaaaga ggtgaagtac atgaaaaaat
      481 taattggcgt acttgtcgtt ttaggcatat ttctcggcgg aagttttgca gcttatcatt
      541 atttttacgg cggcgaagct tattatacaa aaatcacgac gaatggcgaa atgtcagcta
      601 cccaagcaga taacggagag aaattcacga cttataccta caagcaagcg gcttataata
      661 aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag agaacatcca ttaaagatga
      721 atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt attaagctgg gaagaaataa
      781 aggcttctga agtgcctaaa aatgcggcag aaaaaatcga taattagtaa atggtcttaa
      841 aattttatta gataaaaaga ggctgggaca gaagcgttta actccaagaa ataagaagga
      901 gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact
      961 tttgtctcac gctctttttt tgttatattc gtcttttccg atccttctct tgacacaatt
     1021 catttaactg atcactggta cttgaacacc taatattcta gtcattttta taaacgattc
     1081 aatacttcat ccacattttt gatcatcacg gatatcgtac catccggatt attcacaaat
     1141 tcaatcaaat ctgggttgcg atatacatct actggtacga tcagttcgat cccgtttgat
     1201 agcttgaatt tttgttttcc aaattttttc tcagaaattt cttgtacttc ttttacagga
     1261 ggtgcttctg ggacaaatcc ttgctcttgt acttcttctt taaaagccat ttttgccgtg
     1321 atgttttcct tgaagacttt ttccgcaatc atctcatgat ccaaccgacc tttttcttct
     1381 atcgtatcat agacagcttc tttcaaatca gccatgatat caaattcttc tgtctcaaat
     1441 ttttttccaa gatgcttggc tactttttgg atcactttta cattttcttc taaagaaggt
     1501 gcaggaacac tttcgatcac tcttccagaa aaataaagct cttttttgcc agaaaactca
     1561 tatttttttt caattaattc gtaggtccaa tcagtcaaat tcacagcgaa tgcttcatct
     1621 gctttttgtg ttttcgcccc tagaattgct cgattcaaaa tcaagcggtt gtcgatccct
     1681 gcttcgtctg cttcaacaaa gtgagtgtaa gcctcacgat aattgacttt caataaagat
     1741 aagtacatca ctgtatctaa ttcatacaag acaacaaata catctgcact aggtgcttct
     1801 tcactttctt ggtagatatc gaaccaccgc tgaacgattg cttcacttgc ttccacaaaa
     1861 ttttcttgag cgattttaag tgattcaacg atcatgcttt cttctgctag ttgtccagtt
     1921 tttgtttgag cagaagagat tttttggatc ttcttttgaa gaaaatcacg aatatattct
     1981 tttgtcagat ccagctcttt ttgtgaaaag actggagaac cagtttctct gtcaataata
     2041 tgcagaattg ctttttttaa atagatatcc at
//
LOCUS       DAYYAJ010000209         2066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000209 DAYYAJ010000000
VERSION     DAYYAJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.217"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHATZ_002930"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHATZ_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHATZ_002931"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHATZ_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHATZ_002932"
     CDS             1898..2059
                     /locus_tag="ACHATZ_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405383.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          741 a          326 c          356 g          643 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa tttttt
//
LOCUS       DAYYAJ010000210         2053 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000210 DAYYAJ010000000
VERSION     DAYYAJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.218"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..122)
                     /locus_tag="ACHATZ_002933"
     CDS             complement(<1..122)
                     /locus_tag="ACHATZ_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8405384.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSS"
     gene            complement(133..330)
                     /locus_tag="ACHATZ_002934"
     CDS             complement(133..330)
                     /locus_tag="ACHATZ_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8405385.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(353..646)
                     /locus_tag="ACHATZ_002935"
     CDS             complement(353..646)
                     /locus_tag="ACHATZ_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405386.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            complement(683..820)
                     /locus_tag="ACHATZ_002936"
     CDS             complement(683..820)
                     /locus_tag="ACHATZ_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8405387.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(822..1268)
                     /locus_tag="ACHATZ_002937"
     CDS             complement(822..1268)
                     /locus_tag="ACHATZ_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405388.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1265..1414)
                     /locus_tag="ACHATZ_002938"
     CDS             complement(1265..1414)
                     /locus_tag="ACHATZ_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405389.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            complement(1431..>2053)
                     /locus_tag="ACHATZ_002939"
     CDS             complement(1431..>2053)
                     /locus_tag="ACHATZ_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405390.1"
                     /translation="TRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKI
                     SEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERI
                     NDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPIS
                     FEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
BASE COUNT          558 a          424 c          323 g          748 t
ORIGIN      
        1 gaactacctt cgtttacacc taaattaaat tcattgttaa tcgtataggc aaacccatta
       61 atcggcaata aaaaaagagc cgttaatagg ctcatcgcag taatagtaat tttctttttc
      121 atttgtttcc tcctattttt tcaaattata agccgacaca ccagtgataa cacctaaaaa
      181 tgttgctgct gcattgatag tcagaactgc catatctgtt ccattccatc catatgcttt
      241 gcctaatgtg gctactaata cagataaagc tggcaatact gttaaaactg tccatttaat
      301 gacttgataa tacttatcgg gtaaaatcat ttcttcttaa ctcctttctt ttttaccgag
      361 acctttctct aaataaagtt ttaatttgtt gtgtgtgttc taccaatttt tctgcatgtg
      421 tatctaatct ttcatcgtgt ttctttagtt cttcatgaat catcaatcga tctgatttgc
      481 tcgattctaa atctttagtc agcaaatcta aattgtgact tacttttgaa agagtttcag
      541 taatcttcga gaaagatgca gtgactggtc ttattactaa taaaatcaaa gaaacaatcg
      601 cagtaattga tcctgcgatt gttccccatt cccctaaatt gatcatgtga caactccttt
      661 accttaaata aaaacgcatc acttaagatg cgctttcttc tttgctaatg attttatctg
      721 cttcttcgtc tgtaatgcat agtggaacga atagtcgaac ttgatcgtca gtaaaacagc
      781 cccaatcata catcattttc acatcgctaa aactaaacat actactcacc tccctttgaa
      841 gctggattta gttgctcttt aatttctgaa atgtctttgc tattttgtaa cgaagcaagc
      901 atcatttttg aattgatttg tgctaaacta tccgcttttt ctttcaatgc agtattttcc
      961 tgtttaattg ctacatcgct tagcatgagt ttggcattga tctgttttaa atcgccgttc
     1021 tcattttcta acgactcata cattgctttg agattgttta aatcgttgtg atctagtgcg
     1081 ttcgctaaaa taatccattg attcaattta ggatcaaaca tttgatcagc gattgttaac
     1141 ggttcgccat cagcacgaat tccttcaagc ggtggctgat ctgtgtaagg aacggataca
     1201 agcatgtcgt ccaatacttt tcctgcgtac tctccgccag tacgtccata tttccaaatg
     1261 tttttcattt atttccctcc cagtaattga atttcggttt ccaatttaga atcggtggtt
     1321 cgacttctgt gcattcttcc ggtaaatgtt cttcatcatt cacaatgatt tgctcgaatc
     1381 cgtaaggttc aattggtcta tatgctgcct tcatatcgat tcacgctttt ctaaatcgtg
     1441 taagtaattg caaaggtata atccgatcca taacttgagt ttcttcgcca tttaatggct
     1501 ccatctgcac caatagataa ctgagcactg ttcaaagtag aacggtctat cgagccaacc
     1561 agttgctcaa aactaattgg tggccgatag ccttctggaa ttgttagtat cgttgaatca
     1621 tttccaccac tgctttttcc gtttaaagcc acaaaatata tagaaactgt ttttccttca
     1681 cgataaagct ttgctgatcc ggtattcccg tttgtaactg ttaatgtggt agtagctgta
     1741 tcattaatgc gttcatcgat cttattgtct aattcatcta tagcagtcgc attagcattc
     1801 gcttttgttt gagcatcctt agctgtggtg tctacttcat taattgaagc agtcaactgc
     1861 gaattaatct ccgatacttt cccatcggta taattgtttg ctttaccagt aatttcagaa
     1921 attttaatat ctgtggccaa attatcttcg acatattctg gtgctagatc ccaaacataa
     1981 tcttttggat tgtttgaatc acgcatacca gtaccacgat atttatactc actaatattc
     2041 ggggttcgag tat
//
LOCUS       DAYYAJ010000211         1982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000211 DAYYAJ010000000
VERSION     DAYYAJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.219"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..876
                     /locus_tag="ACHATZ_002940"
     CDS             <1..876
                     /locus_tag="ACHATZ_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8405391.1"
                     /translation="ILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQ
                     PGYVQYGAGSYANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPV
                     IDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVKADGIYRPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPQR"
     gene            1133..1780
                     /locus_tag="ACHATZ_002941"
     CDS             1133..1780
                     /locus_tag="ACHATZ_002941"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8405392.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
BASE COUNT          731 a          278 c          386 g          587 t
ORIGIN      
        1 attttactgc atgaaacggc taatgaaaca gcaacaggac gcaatgaagc gcagtatatg
       61 caacgttcat ggactagcgc ttatactgct tatattgtgg gagacggcgg aattgtttat
      121 caagtcggtc aacctggtta tgtacagtac ggtgctggtt cgtatgctaa tgcaaatagt
      181 cctgtgcaga ttgagttaca acacacacat gataaagcaa cgtttgagaa aaactacaag
      241 gcatacgttg aattagctag agattcagca atgaaatatg gtattccact tacactagac
      301 acaccgtata atcaaccagg gatcaaatcg catttatggg taacacaaaa tatttggggc
      361 gatcatacag atccttacgg ttatctttct gaaatgggcg taagtaaaga aaaattagcc
      421 tatgatttgg ctcatggatt taccgatgaa aatccgacaa cttcagatga taaaccagtc
      481 attgatccaa ctcgagcagg tgctgcaaat cctaccctga cagatggaac aaattacgcc
      541 cacattgatc agttcggaga aatcgaaaac gcaaacttac atgtggctgg ttggcacatc
      601 gctaactata aatacgagta tatcttcatt atggattaca atactgggaa agaattagct
      661 cgagtaaaag ctgatggaat atatagacca gatgtaaatc aagcttataa tactttagga
      721 aatgttggct atcatgtatc ttttaacatg cgcaacttcc ctaataagaa agtctatgta
      781 atgatgcggg caacgaatga tccagaaggg aacactaaag gcggagcaca agattttcat
      841 gataaacgct ggtatttaaa tattccgcaa cgataaaaat agcccctcgt ttttgagggg
      901 aagtacataa tgcaaatttg tccataactt attgattatc ttgtaaaaat gcggtaattt
      961 aatagttagt gagaagaggt gcttaattat agttaaaaaa gaggaaatag aagcagcatg
     1021 taaccaagca aatgttaggt ttattggaga agctttatct ggatatccta agtataaata
     1081 cttacacaac tggttacaaa gtaaaagttt ttatcttaat aaagaaagat gcatgcctaa
     1141 taaaaattat agggtttata aaagaggaac tattgtatat gttgattttg gggtgaacgt
     1201 gggttatgag ttatctggta atcattttgc tattgtttta aataataaag ataattcgaa
     1261 aaatggtgtt gtatgtgtag taccaatctc ttcaaaaaag aaaagtaatt atgtatctgt
     1321 aggaaaaata ttagaagttg ctagtattaa acaatttata agccaagctg ataaattaaa
     1381 agagaaactt aggataatta ttttactttc attaaataga ggcatcattg atgaaagcga
     1441 tttgaatagt tttattaaag atattgaagc taaaggtgaa aagctttcag tggaagaagt
     1501 agaaaaacga gctgtaaaac ataatttggt ttgcgagaca agggaacaag taattaaaga
     1561 aataaaatca ttatctgaag atatggtaaa atatcagaag gtgtttaatt gctacaagaa
     1621 atatacacaa gaatcctatg taatgccact taatatccaa acgattagta aaaatagaat
     1681 tagaagaatt aacaagtttg atccttctgg aaatatgaag gcacctggaa atgtcctcaa
     1741 tgaagtagat gaaagtttaa aaaaatattt tacaaagtaa acgatatttt tgttgacatt
     1801 tttctgttcg ttagttacca tgtagttata gaaggacgta agcgcctatt tttaatgtac
     1861 acatcgggac gtatgcgccc acatagaaac atagttgaga gacatctata tggtgtctct
     1921 ttttttatta atatgcaaaa aagtctagtt ttttcaataa tatttatgta ataataaatt
     1981 ag
//
LOCUS       DAYYAJ010000212         1969 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000212 DAYYAJ010000000
VERSION     DAYYAJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.220"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..419)
                     /locus_tag="ACHATZ_002942"
     CDS             complement(<1..419)
                     /locus_tag="ACHATZ_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8405393.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDL"
     gene            complement(416..1069)
                     /locus_tag="ACHATZ_002943"
     CDS             complement(416..1069)
                     /locus_tag="ACHATZ_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8405394.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1357..>1969
                     /locus_tag="ACHATZ_002944"
     CDS             1357..>1969
                     /locus_tag="ACHATZ_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8405395.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          619 a          351 c          251 g          748 t
ORIGIN      
        1 cttaaatctt tcgcatcttt ttgcatatca gctgtagaaa caaattgtag atttgttttt
       61 ttaccatcat taagctttat ttctctttct tcaattatca aagaattact atcactttct
      121 aaattaacat ttatattatc tttgatttgt atttcattct tattattttt ttcttttatg
      181 aatactttta tttcactact cttggaataa aactccaaag tttgttctat atattttatt
      241 tcttttccat tcatattact agaaatttca tttgccatat tatgaatctt ttcttttctc
      301 gtctccaaat atgttttagg aaagataaaa aaaattaatg aatgaactaa tataattatt
      361 attccaagaa cagaaaatat ttgtatgaac atttttggaa atatctttaa ttttttcatt
      421 ttctctccaa tttatatcct acatttctta tagtagttat acagtctaat tgtaattttt
      481 ttctaagttc ttttatatat acatctatta ctcgatcata aggagtttct tcgctatctt
      541 tccatacata atcaataatt tgcattcttg ttaacacttg tccattatta tccaataaac
      601 attttaatac ttctagttct tttgcattta catctatttc ttcaccattt attttagctg
      661 tatagctatt aaaattaacc gatagatcct tatattcaaa cttctctaaa tgtccataat
      721 tcttcttaat taaagaatct attctagctt ttaagaccgg caatgaaaat ggcttttcta
      781 catatccgtc tactaaatta gtaaatgcat caattttata ttcttcatca ctaaatgcag
      841 tcaagattag aattggtaaa ttgcttttct ttctaatttc tttcaacact tctaaaccat
      901 ttataaaagg tatctgaatg tctaagataa ccaaatttat atcgctatta aattttgaca
      961 aggcttctct tccatcttta gcttgaataa cagtatatcc aaattctgaa agatattcac
     1021 ttattccctc tcttatcata ttatcatctt caacagttaa tattttcata tatacctcca
     1081 cttaagccta tacactagat attatacagc atattatggc tctataatat ctagtgttgg
     1141 tgacaccttc tgttgccaat actttttctt ttttctaata aatgcaaaca gacaccttga
     1201 aatcctttag aataaagggt tctataatat tttgtgttgt tggaaaatcg aaggcgattt
     1261 tctactaaca ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaaatttag
     1321 tcgaccaaaa catcacccta ccaaaggagt cgctctatgg ataatcatac tagaaaatta
     1381 ctcgtattaa cagataaaca tttatttttt gatgaagaat ggctgattga aaaagaatat
     1441 aaaggcgttc aagcgcagtt tattaaaggg agattggatg actcaccttc ccattgtgaa
     1501 cactgtggat cgattcgtat tatccgcaac ggttcctata cgacacgtac tcagatattg
     1561 aaagtcaaag aaaagttgac tattttagaa ttaaaacgta cccgttttct atgctatgat
     1621 tgtgggcaga ccttttctgc gaaaacggac ttagttgatg aacatcatca actaacaaag
     1681 gaactgaagc aggcgattct catagaactg tacgaaaatc aatcacgtaa actaatcgct
     1741 aagaagtact tcgtctcgga tgggacggtg acacggatct tgcgtgaagc aacgaagcat
     1801 taccagccac gtatgaactt ccttccgacg gtgttgtgta tggatgagtt caaatcaatg
     1861 aagtctgttt ctggttccat gagttttatt tgtgttgatg ggacaaccca tcagctattt
     1921 acgatccttg aagatcgcag actttacaaa ctaactcaat acttcctgc
//
LOCUS       DAYYAJ010000213         1962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000213 DAYYAJ010000000
VERSION     DAYYAJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.221"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHATZ_002945"
     CDS             complement(<1..426)
                     /locus_tag="ACHATZ_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8405396.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHATZ_002946"
     CDS             complement(535..1566)
                     /locus_tag="ACHATZ_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8405397.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          605 a          325 c          339 g          693 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt ac
//
LOCUS       DAYYAJ010000214         1961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000214 DAYYAJ010000000
VERSION     DAYYAJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.222"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHATZ_002947"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHATZ_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8405398.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHATZ_002948"
     CDS             853..1041
                     /locus_tag="ACHATZ_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8405399.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHATZ_002949"
     CDS             1054..1611
                     /locus_tag="ACHATZ_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8405400.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHATZ_002950"
     CDS             1643..1885
                     /locus_tag="ACHATZ_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8405401.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          305 c          404 g          536 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt t
//
LOCUS       DAYYAJ010000215         1905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000215 DAYYAJ010000000
VERSION     DAYYAJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.223"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..132
                     /locus_tag="ACHATZ_002951"
     tRNA            59..132
                     /locus_tag="ACHATZ_002951"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:93..95,aa:Ile,seq:cat)
     gene            680..1654
                     /gene="bsh"
                     /locus_tag="ACHATZ_002952"
     CDS             680..1654
                     /gene="bsh"
                     /locus_tag="ACHATZ_002952"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8405402.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          627 a          310 c          352 g          616 t
ORIGIN      
        1 ccattattat ctagtctatg tccattcttt ttgtttgcag tagttgtgat ggctttccgt
       61 actcttagct cagttggtta gagcagacgg ctcataaccg tccggtcgta ggctcgagtc
      121 ctacagggta catactctaa gtgatttaaa ttgcgaataa aatataaaaa tgactcccct
      181 tttgactccc ccaagcagta tatggatgtc tctaaaagtt gtttttttag ataataaaaa
      241 gctgataata agcgaaaaac acttaaaatc agctttaaac cctattctcg aaatccggcg
      301 aaccggctaa taccggcgcg caggttcaaa tcctgtacct tccttatatc aacgattaga
      361 ggttgtgaag aagctgtcct gcatcaagta ataagaacgg ccaaacaaaa atagcttttc
      421 ctattttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt
      481 cggaataagg ggctgtgaca agacttttgt cacagcccct tattgttgta cttttaacct
      541 acaatatttt ttgattcccc ctgagtacta ttttttataa atgctagtaa atcaatcttc
      601 tttggccttt tttttgataa aaaaaaatac tagatgtaag ataaaagtgt aaaaacgatt
      661 tgttttagga gggaaaacga tgtgtaccgc aatcacttat gcaacgaaag accattattt
      721 tggaaggaat tttgattatg agatgtctta taatgaagta gtcactatta ctccaagaaa
      781 ttatcgtttc gattttagaa aggtaaaaaa tttagataaa cattatgcaa tgataggaat
      841 agcagctggg gtatccaact atccactcta ttatgaggct acaaatgaaa aagggttgag
      901 tatggcagga ctgaattttc ctggcaatgc tgattataaa gaattacaag aaggaaaaga
      961 taacgttgca ccatttgagt ttattccatg gatactaggg caatgctcaa ccatagacga
     1021 agctaaagaa ttattggcta ctatcaatct agtcaatatc gattttagtg aaaaactacc
     1081 cttgtcacct ttacactggt tattagctga taaagaaaaa tcgatagtaa ttgaaagtat
     1141 gaaagatgga cttcatttat atgataatcc tgtcggtgtg ttgacaaata atccgccatt
     1201 tgattatcaa ttatttaatc tgaacaatta tcgctcgcta tcaaatggaa caccagaaaa
     1261 tcatttttca aaccagatca gcttgaatgt ttatagtcgt gggatgggag gattgggctt
     1321 acctggagat ctctcttctg tttctcgatt tgtaaaagcc acatttacta aaatgaatgc
     1381 tgcatcaggt gattcagaat ctgaaagtat cagtcaattt tttcatatac taggctcagt
     1441 cgagcaacaa aaaggagtat gcgatactgg agaaggaaaa tacgaatata cgatttattc
     1501 ttcttgctgt aacgttgata aagggattta ttattatcgt acgtatgaag acagtcaaat
     1561 tactgctgta aatataaata aagaagactt ggatcgtcat gaattggtca gttatccagt
     1621 cataaaagag caacagataa actatgtcaa ttaattaatg aatagatccg tttggtatca
     1681 tattttctga tacagtcata attagacacg atctttaggt cgtgtctttt tttgctgttt
     1741 ttttggaaat agcatccact aatatttttt taacacgaag agaataaggt agcctcaaaa
     1801 aagaaaacat ggtaaattag atattgggac tttattttct gtaaaaacag aaactttcat
     1861 cttttgtttt cacgtgacga aattgtaaga gagaaaatat tcgtc
//
LOCUS       DAYYAJ010000216         1897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000216 DAYYAJ010000000
VERSION     DAYYAJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.224"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..809)
                     /locus_tag="ACHATZ_002953"
     CDS             complement(<1..809)
                     /locus_tag="ACHATZ_002953"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM8405403.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSI"
     gene            complement(781..1677)
                     /locus_tag="ACHATZ_002954"
     CDS             complement(781..1677)
                     /locus_tag="ACHATZ_002954"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8405404.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1667..>1897)
                     /locus_tag="ACHATZ_002955"
     CDS             complement(1667..>1897)
                     /locus_tag="ACHATZ_002955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405405.1"
                     /translation="GLPIISNLSGDTKEIVKRYNVGINYNSTVDENEMLKFYQCNKNI
                     NENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          659 a          319 c          236 g          683 t
ORIGIN      
        1 ttgatagaaa atatatatga gcttaagctg ataacttttt tttcgtaaat attcattttt
       61 gataatttat gaactaaaaa aagatttgct gaaaaaaaga gagcaaacat cactacgtga
      121 gaaatcgatg tactgtttct atcaaaatat gcatcatact tcgtatttat aaatattgaa
      181 ctgatataac tttgtccaat agagaatagg agcaagctaa ggattgtaac atatccagta
      241 atctttttat taatttttac aataaaaaaa gggagataaa aatatgcaag attatggaac
      301 agtccagcca ctaagataaa aaaaatatac ttaaatatag ctttgctgcc tcgcaattta
      361 gttagatgat aaaaagctag taatatgaat atcaatgcgc taaacatacg tacttgtatt
      421 gcttgtgaag taaaaccaaa aataaaaaac aagaacaaaa ctaaaaaaac attagttgtg
      481 aataatctga tgacatacaa taaagctgag agtaagacag tagaaataat caataaaaaa
      541 atctgatact gactggaaat aaaggtacat acgtacataa cagctctgaa tcccggttca
      601 atactatctg aactaaagtc cataccaaat tgtgctatcc cattatacaa gacagaatat
      661 gcataataat cagcgttttg tgtgttaaat gccatgatga tccacattat caaaagtagc
      721 accccagaca aaatcttgtt cgatgtactc catttttttt gtgaaacact cttttcttga
      781 ttaatctcca atgacgcctc tgtatccatc tcgaaccccc cttatagccg ctctaaatct
      841 actgagctta tcagtctcaa aagctattgc aataaaaaat ttcaataatc ttttattcat
      901 aaggcgggaa aattccttat cacttttata cattcttttc accaatgttg aatttcttgc
      961 aataaaatac tttcgtgttg aattatgatt gtctatttta attttttttg atcctatttt
     1021 taaattttct ccatctccaa cgtgatgata aaaatagact tgatttgtta cataaatgtt
     1081 atagccatta tttgcaaccc ttttacaaaa ttcaacatca atctgatcaa taaaaaattc
     1141 atccttcata tttccaacat cgtcaattaa ttcacttttg aaaaacattc cagaggaatt
     1201 tgcaaatgct attttcatgg aaccactagt tgaagcaaag tgttttttta cgtatctccc
     1261 tctctcattt ttgcaggtaa aagagacaga ttcatcattg ctattctcaa agtaattggg
     1321 aacaataatc ccaatgtcct tatctttcgc ttcaatattg gtaaactctt ctaataattt
     1381 taaagaaaga ttcttttgta tgatggtatc ttgatctagt aaaaggatat aatcacattt
     1441 ctcttttttg gcaatttcaa tacctatgtt ttgagctgtc gcaagcccaa tgttctcatt
     1501 catttttttt aaaattaagt tttccatcat ttcaaattga aaagagttaa tattatctga
     1561 gccgttatcc acgataataa gtttgttctg tttctcttgt attaacgtat ccactaataa
     1621 ctttatgtta ttatctggat tgtaggtaac aataattatc ccaattttat ttttcatcgt
     1681 tcatcacctt gtacacttca ttccatttca ttttcaatac ttgctcagag aatatagtgg
     1741 cgtaaatttt tctaacattt tcatttatgt ttttattaca ctgataaaat ttcaacatct
     1801 cattttcatc aacggtacta ttataattga ttccgacatt atatctttta actatttctt
     1861 tcgtatctcc tgataaattt gaaattatag gtaatcc
//
LOCUS       DAYYAJ010000217         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000217 DAYYAJ010000000
VERSION     DAYYAJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.225"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHATZ_002956"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHATZ_002956"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8405406.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYAJ010000218         1815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000218 DAYYAJ010000000
VERSION     DAYYAJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.226"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..122)
                     /locus_tag="ACHATZ_002957"
     CDS             complement(<1..122)
                     /locus_tag="ACHATZ_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8405407.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSS"
     gene            complement(133..330)
                     /locus_tag="ACHATZ_002958"
     CDS             complement(133..330)
                     /locus_tag="ACHATZ_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8405408.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(353..646)
                     /locus_tag="ACHATZ_002959"
     CDS             complement(353..646)
                     /locus_tag="ACHATZ_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405409.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(683..820)
                     /locus_tag="ACHATZ_002960"
     CDS             complement(683..820)
                     /locus_tag="ACHATZ_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8405410.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(822..1268)
                     /locus_tag="ACHATZ_002961"
     CDS             complement(822..1268)
                     /locus_tag="ACHATZ_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405411.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(1271..>1815)
                     /locus_tag="ACHATZ_002962"
     CDS             complement(1271..>1815)
                     /locus_tag="ACHATZ_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405412.1"
                     /translation="TRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNDAVNV
                     YDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVIV
                     NGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYVRKDLGTIITN
                     SEFAANEWFTFHGNYWVGEE"
BASE COUNT          505 a          382 c          272 g          656 t
ORIGIN      
        1 gagctacctt cgtttgggcc taaattaaat tcattgttaa tcgtataggc aaacccatta
       61 attggcaata aaaaaagagc cgttaatagg ctcatcgcag taatagtaat tttctttttc
      121 atttgtttcc tcctattttt tcaaattata agccgacacg ccagtgataa cgcctaaaaa
      181 tgttgctact gcattgatag tgagtactgt catatctgtt ccattccatc catacgcttt
      241 gcctagtgtt gcaactaata cagatgcagc tggcaatact gttaaaactg tccatttaat
      301 gacttgataa tacttatcgg gtaaaatcat ttcttcttaa ctcctttctt ttttaccgag
      361 atctttctct aaataaagtt ttgatttgtt gtgtgtgttc taccaatttt tctgtatgtg
      421 tatctaatct ttcatcgtgt ttctttagtt cttcatgaat catcaatcga tctgatttgc
      481 tcgattctaa atctttagtc agcaaatcta aattgtgact tacttttgaa agagtttcag
      541 taatcttcga gaaagatgca gtgactggtc ttattactaa taaaatcaaa gaaacaatcg
      601 cagtgattga tcctgcgatt gttccccatt ctcctaaatt aatcatgtga caactccttt
      661 accttaaata aaaacgcatc acttaagatg cgctcttatc tttattaatg attttttctg
      721 cttcttcgtc tgtaatgcat agtggaacga atagtcgaac ttgatcgtca gtaaaacagc
      781 cccaatcata catcattttc acatcgctaa aactaaacat actactcacc tccctttgaa
      841 gctggattta gttgctcttt aatttctgca atgtctttgc tattttggaa cgaagcaagc
      901 atcgtttttg aattgatttg tgctaaacca tccgcttttt ctttcaatgc agtattttcc
      961 tgtttaatcg ctacatcgct tagcatgagt ttggcgttga gctgttttaa atcgccgttc
     1021 tcattttcta acgactcata catcgctttg agattgttta aatcgttgtg atctagtaca
     1081 ttcactaaaa caatccattg gttcagttta ggatcaaaca tctgatcagc aatcgttagt
     1141 ggttccccat cagcacgaat tccttcgagt ggtggaacat cggtaaatgg aacagtcata
     1201 accatatcat caagcacttg tccagcatac tctccacctg ttcgtccgta tttccaaata
     1261 tttttcattt ttattcctcc cctacccaat aattaccatg gaatgtaaac cattcatttg
     1321 cagcaaattc gctattcgta ataattgttc ctaagtcttt tcgcacgtac atcgcttttg
     1381 atacactact tgttcctgac ggacctgtaa gcatgccatg aacaattttt gcgacaaatt
     1441 catctgggac ttcatcatca agtggtacac caaaagcata agcttttttg aactttactg
     1501 taccatttac aataacttct tttccgtgtt taataaatgt tgcataccca tcgataaacg
     1561 attgactgtt gtctttagtt aatgtataaa cagtgcaatc attttcactg atcactttat
     1621 ctcctgctat ttgaatacca tcggcaaagt ttttcaaacc ttcaactctc tgaggatcat
     1681 acacattaac agcatcattc aagccttttt cagtatattc aggtgtgaca tcccaactgt
     1741 aatcattggg attgttactg tctttcaagc cttcaccaaa gtatttaaac tgactaatat
     1801 tcggggttcg tgtgt
//
LOCUS       DAYYAJ010000219         1803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000219 DAYYAJ010000000
VERSION     DAYYAJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.227"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACHATZ_002963"
     CDS             <1..790
                     /locus_tag="ACHATZ_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8405413.1"
                     /translation="TILRELKKEFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDP
                     KDYLQQATSAAETIAEQLKRLSVDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETE
                     LLRTTAGNEKYRFITLSSIEHFPLTLANYFFVQKNQLPVTKAFKELAELIGDVNESYF
                     FDQIEVIVDRIRKNKYKNRRPSIFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPT
                     LDPSLTMEEMAFLKRQIFSELSKKFIPHAFSYIKEYGTVLVSKT"
     gene            924..1049
                     /locus_tag="ACHATZ_002964"
     CDS             924..1049
                     /locus_tag="ACHATZ_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8405414.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1183..>1803)
                     /locus_tag="ACHATZ_002965"
     CDS             complement(1183..>1803)
                     /locus_tag="ACHATZ_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405415.1"
                     /translation="RRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDH
                     SDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAIL
                     FYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFF
                     VLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          690 a          269 c          346 g          498 t
ORIGIN      
        1 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
       61 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
      121 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
      181 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
      241 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
      301 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
      361 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
      421 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
      481 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
      541 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
      601 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
      661 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
      721 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
      781 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
      841 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
      901 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
      961 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
     1021 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
     1081 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
     1141 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
     1201 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
     1261 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
     1321 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
     1381 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
     1441 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
     1501 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
     1561 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
     1621 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
     1681 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
     1741 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct
     1801 acg
//
LOCUS       DAYYAJ010000220         1746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000220 DAYYAJ010000000
VERSION     DAYYAJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.228"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..603
                     /locus_tag="ACHATZ_002966"
     CDS             <1..603
                     /locus_tag="ACHATZ_002966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8405416.1"
                     /translation="IVMNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKI
                     ITWRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNV
                     YKDKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEV
                     YLAGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            584..1030
                     /locus_tag="ACHATZ_002967"
     CDS             584..1030
                     /locus_tag="ACHATZ_002967"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8405417.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1373..1696
                     /gene="relB"
                     /locus_tag="ACHATZ_002968"
     CDS             1373..1696
                     /gene="relB"
                     /locus_tag="ACHATZ_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8405418.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
BASE COUNT          662 a          231 c          318 g          535 t
ORIGIN      
        1 attgttatga atccaacatt gattactaag aaagaattac taaaaaaatt agatatttct
       61 actggtgtat tagctaattt aattagaaat ggcatgccaa aagagggaga aatgtttaat
      121 cttgataaga ttattacatg gcgagaaaat tggtctaaaa acatattagg tgagttagaa
      181 gttggtagag tatatactaa caaagaaatt tcagagaagt ttaaatgttc caaacagggt
      241 ggaatgagaa gatctcatca aactaatact cttgtactgt ttagtgatca aacaggaagt
      301 aatgtttata aagataagtg gttaaatggt attttacaat ataccggtat ggggctaaaa
      361 ggggatcaag tattggataa gaatcaaaat aaagttttag ccaattcaaa aagtaatttt
      421 gtaaagattc atttatttga aacatttaaa ccaaaagaac acacatatct gggagaagta
      481 tatttagcag gacaaatata tactgttaat gaaaaagata gttcgggcaa cagcagaaaa
      541 gtatataaat ttccattagc tttaataaat caagagcaat tgattgaaga taaagatata
      601 taaccaggaa gaaaatcaaa ctagacatat tcgtaattta tctaatgcta aattagagga
      661 agaagcaaga aaagttagta attataataa aaaaatggct gcaaaatatg ttaacgaaga
      721 ggagttttgc ttctcaaaaa caaaaatata taaacgtaat cccttcgtat ctgaatatgt
      781 taaacgactt gcaaaaggta tttgtcaact ttgccaagaa aaagggccat ttataaaaga
      841 cggtgtacct tatttacatt gtcatcatat agaatatttg tcgcaaggtg gaaaagatgt
      901 gattgaaaac tgtatcgcgt tatgtcccaa ttgtcatgct agaattcatg aacttgagtt
      961 acctgacgat aaagaaaaat tgctgaagat cgttgaaaaa agagaaaata gcttttctaa
     1021 aataccgtaa tttattttta taggacaaat aagctaatgt gagctatgcc ttctatataa
     1081 gactaagttc tattataagt aacgaagtaa ttctcgttcc ttcctgtagt aatatttttt
     1141 aaataaccta taggcttctt caaaagtaca tcctccagta aattctaagt actggaggat
     1201 tctttttcta gtgttgcttt actcatgaaa aagtagttgg ctacttcatc aaaatttctg
     1261 aactttttta atgtataata aagagctcga tcatttacta caaggatgct agctcctaca
     1321 ttttcacagt tttttagcaa gtaaattttt ctaaaatcac atcaggaaat gcatgagatt
     1381 ctttaagaga ggcaattata gggctatgat tacggttcgt gtaagtgacg ccaaaaaaga
     1441 atggctttct tatatggctg aattttatgg gattacttta tctgaacttc taaaaaacta
     1501 ttcgatggaa caactagaag atgaatacaa tcgtcaaacg gctgaaattg cccataaaca
     1561 ttggataaga agtgggaaaa caacagtatt catggaagaa tccttaatga atttggtggg
     1621 ctggaatgac ctgccgttta aaattcacgc ccgaaattca gaagcagctc tgattggcaa
     1681 atacgtgtat tggtagagat agaagataat caattcgttg ttatcgctat tcaagtaggg
     1741 caccga
//
LOCUS       DAYYAJ010000221         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000221 DAYYAJ010000000
VERSION     DAYYAJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.229"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHATZ_002969"
     CDS             131..505
                     /locus_tag="ACHATZ_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8405419.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHATZ_002970"
     CDS             complement(619..1248)
                     /locus_tag="ACHATZ_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8405420.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHATZ_002971"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHATZ_002971"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYAJ010000222         1639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000222 DAYYAJ010000000
VERSION     DAYYAJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.230"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1594
                     /locus_tag="ACHATZ_002972"
     CDS             128..1594
                     /locus_tag="ACHATZ_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8405421.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          460 a          296 c          337 g          546 t
ORIGIN      
        1 attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt aaataagttt
       61 tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa gaattggggt
      121 atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg gattgtcaac
      181 tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg cattacttat
      241 ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag caacagcatt
      301 agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg gcggatgggt
      361 atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt tcattatgct
      421 tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt cgatggcatt
      481 gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg gacatcttta
      541 ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg gtgtcaactt
      601 aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata actatcattt
      661 agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca tcatccaagg
      721 acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa ctgaagaacg
      781 taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag tattcggcgg
      841 ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca gtgtattggg
      901 gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg taactgctga
      961 agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca ttttctggtc
     1021 attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta cacaatctac
     1081 gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa ttttcatctt
     1141 tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc aaccatctac
     1201 agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc tattgatgtt
     1261 gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt tgggaagttt
     1321 cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa
     1381 attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag ctgatgcttc
     1441 ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag aatctgctta
     1501 cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga
     1561 accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa caggaattgc
     1621 ttatttgcag cagttcctg
//
LOCUS       DAYYAJ010000223         1638 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000223 DAYYAJ010000000
VERSION     DAYYAJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.231"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..400
                     /locus_tag="ACHATZ_002973"
     CDS             2..400
                     /locus_tag="ACHATZ_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8405422.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1078
                     /locus_tag="ACHATZ_002974"
                     /pseudo
     CDS             384..1078
                     /locus_tag="ACHATZ_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          620 a          238 c          323 g          457 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc agcgataacg ttaggtaaag agctactaga
      961 tcaagtatta gagatgccct cgattgataa agcagtgagg ggcttccaag aagattgggt
     1021 gcaaactttc caagcagaaa cgattggtga actaaaaaag gtcgctgatt acttctgaat
     1081 caatttctcc ataaaataaa cgatagcctt ctcgaaaact cgaggaggct atctgacgat
     1141 tcaatattca ttaatttaaa gtttgggtga ctattactaa ctattactta tttctcattt
     1201 aaacgatgta ttccctaacc tctaatacat ccttgtaact ctgaagttac gccagttttg
     1261 aataaatttc ataaaaacaa atgaaaagtg tcgcataaaa atatctttcg tgtttctcta
     1321 gtataacaga ataagaagaa caagtccaga agggatattt ttagtctttt ttaaaataac
     1381 ttttcagtcg agtttgagtt tatgatagtt gtttttgaag cgatacggca agtgaaaaaa
     1441 tagggaagca tgaaagaatc tacttaatgg cgcattgatt catgtgatag aaataaaaaa
     1501 cttttttaaa ttatatcctc atgattgtaa caaatccagt gatttgtttt ggtacaaata
     1561 aaaaaaggcc ccgatgaagt tgtggcaaaa gtggtttgcc tcaaaaataa gaaggaattt
     1621 ccaaaaatca cttctcaa
//
LOCUS       DAYYAJ010000224         1627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000224 DAYYAJ010000000
VERSION     DAYYAJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.232"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHATZ_002975"
     CDS             <1..1604
                     /locus_tag="ACHATZ_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8405423.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          595 a          267 c          332 g          433 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgt
//
LOCUS       DAYYAJ010000225         1589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000225 DAYYAJ010000000
VERSION     DAYYAJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.233"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHATZ_002976"
     CDS             complement(138..428)
                     /locus_tag="ACHATZ_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405424.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHATZ_002977"
     CDS             complement(546..806)
                     /locus_tag="ACHATZ_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405425.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHATZ_002978"
     CDS             complement(943..1356)
                     /locus_tag="ACHATZ_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8405426.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          599 a          253 c          232 g          505 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaac
//
LOCUS       DAYYAJ010000226         1559 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000226 DAYYAJ010000000
VERSION     DAYYAJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.234"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..627
                     /locus_tag="ACHATZ_002979"
     CDS             61..627
                     /locus_tag="ACHATZ_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8405427.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            772..859
                     /locus_tag="ACHATZ_002980"
     tRNA            772..859
                     /locus_tag="ACHATZ_002980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:804..806,aa:Ser,seq:cga)
     gene            complement(911..>1559)
                     /locus_tag="ACHATZ_002981"
     CDS             complement(911..>1559)
                     /locus_tag="ACHATZ_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8405428.1"
                     /translation="YTKEELIKFLEAIRNDDDERYLFFSLLAFTGIRKGEAFALTWSD
                     IDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLLRKYYTKNKTIVT
                     IQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHTHASLLFESGASL
                     KDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGESKGESKNILG"
BASE COUNT          504 a          246 c          291 g          518 t
ORIGIN      
        1 tgctaaaaat agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta
       61 atgagaagaa aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca
      121 aaggaagggt ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca
      181 cagccgattt atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct
      241 atctttgaac aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt
      301 gatctcggtt tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg
      361 tacattgatg aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt
      421 gaaatagtta aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg
      481 catactggta catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg
      541 catccaacag aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca
      601 ttagataatc ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc
      661 gttggctgca aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct
      721 attagaaata ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg
      781 gcagagtggt aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt
      841 tcaaatcctg taccttcctt atatatcaac gattagacac gatttcgatc gtgtcttttt
      901 tgttgtatat ttagcctaaa atgttttttg actccccttt tgactccccc tgagtactgt
      961 tcttcataaa tgctacaaaa ttattgatga cttttttgtt ttgagtttca gttacatgag
     1021 tgtaaatatt tgaagtagtt tgaatgtctg catgccctaa acgttcctga acatctttta
     1081 aagaagctcc tgattcaaaa aggagagagg catgagtatg tcgaaaacca tgagtagtga
     1141 ttcttttagt caattctgga tgatgcttat atatgacatt aagccacgaa cgtgaaactg
     1201 tagggttata gagaagacca tcatggtgaa aaattaagtt ttccctttgg attgtaacga
     1261 tcgttttgtt ttttgtataa tactttctca ataaactgat caaatcattg tccagataaa
     1321 tttttctttt tccagatttt gatttaggag tatttacgat caatcttcct tgataacctc
     1381 ttgttacagt tttattgata ttgagtgttt tgctcttaaa gtcaatatcc gaccaggtca
     1441 gagcaaaagc ttctcctttt cttatacctg taaaagccaa tagggaaaag aataaatatc
     1501 tttcatcatc atcgtttctt attgcttcta aaaatttgat taattcttct ttagtataa
//
LOCUS       DAYYAJ010000227         1521 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000227 DAYYAJ010000000
VERSION     DAYYAJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.235"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..464
                     /locus_tag="ACHATZ_002982"
     CDS             42..464
                     /locus_tag="ACHATZ_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405429.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            474..677
                     /locus_tag="ACHATZ_002983"
     CDS             474..677
                     /locus_tag="ACHATZ_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405430.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            888..1472
                     /locus_tag="ACHATZ_002984"
     CDS             888..1472
                     /locus_tag="ACHATZ_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8405431.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          540 a          220 c          284 g          477 t
ORIGIN      
        1 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
       61 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
      121 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
      181 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
      241 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
      301 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
      361 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
      421 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
      481 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
      541 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
      601 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
      661 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
      721 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
      781 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
      841 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
      901 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
      961 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
     1021 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
     1081 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
     1141 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
     1201 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
     1261 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
     1321 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
     1381 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
     1441 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
     1501 gccatttatc tagaaaagtg a
//
LOCUS       DAYYAJ010000228         1488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000228 DAYYAJ010000000
VERSION     DAYYAJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.236"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            428..1468
                     /locus_tag="ACHATZ_002985"
     CDS             428..1468
                     /locus_tag="ACHATZ_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8405432.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          580 a          197 c          235 g          476 t
ORIGIN      
        1 ttgtattttt tagtagtata taatctattg ttttctagcc taaagccttg tcttataaga
       61 ataatataat atatactact gaaataaggt agtatatatt actagtaatg gtagtatgtt
      121 ttatatgcat aaggtagttc ttaatttatt ttttgttttg acactggtag tacttaatta
      181 tctttaattt taaaactaaa ttttattcat acgtttttct ggtagtattt aattataaat
      241 aatatttatt taaagtagtt cttaatgact ttaaaggtag tacataattc attttatttc
      301 tgattttagg tagtatttaa ttaaaaaaca gttttaatgc gtttgcattt tttatttatc
      361 tttgctattt tcgaaaagag taggtaattt tacataaata aaacaacgtg aaagtagagg
      421 atctacaatg actgatttta aattttttaa ggcggataga gtttacaacg aattatttta
      481 tcaatttcca aaagtcttta ttgtttctga cgaatacaaa aaaatgaaag attcaactaa
      541 gattgcctat atgcttttaa aagcaagatt agagatcgca atcagcaaac ggcaaatcga
      601 tgaagaaggt aatgtttatt ttacttatac gacaaatgaa ctatgtagag tattaaactg
      661 ccaaaaacaa aaagcgatag caatcaaaaa agagttggaa tcctttggtt tattattaca
      721 aaagcagatg ggatttaaca aacagttagg gaaaaataat cctaatagac tatatctagc
      781 agaattaaaa gtctcagaaa atgatatcta cttactcgaa aaatttgata gagagaatag
      841 ggaaaacgtt gataaatcag agggtatgaa aatcataccc accctcgacg aaaaatcaga
      901 cgctgaatcc cttggggctc aagagggtat gaaaatcata ccgtgccaaa acgttgataa
      961 atcagagggt atgaaaatca taccagaact taataataat atattagaca ctaatagaca
     1021 caatatagac actgaaaaag accgcctaca agatcaattg ttgttagaca attttgagac
     1081 aattatgaca aacgacagca ttgctacgtt tgtccctgaa cgagtattaa atttgataaa
     1141 aacattttct tcaagttaca gtgaagctca aaaaaccgtc cagactattc ataatgcaaa
     1201 gaaaaaagct gaaatagaaa gtgatatttc gatagttttt gaagaactcg atagttatta
     1261 tgtcaatgca gaacaagaat tatacacgac actgttaaaa gcctatcaaa aattaaaaac
     1321 cgaaaaagtc gaaaatatcc agaacctgat ttttgtctat gtaaaaaatt ggtttatcga
     1381 aaaaccaata gctgctaaag tatcaagtga aaaacgtttg aattatgcaa gctccccaag
     1441 cactattacg aaagactggt tagagtgaaa ctttactgac aaagtgat
//
LOCUS       DAYYAJ010000229         1466 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000229 DAYYAJ010000000
VERSION     DAYYAJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.237"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHATZ_002986"
     CDS             <1..156
                     /locus_tag="ACHATZ_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8405433.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHATZ_002987"
     CDS             175..1452
                     /locus_tag="ACHATZ_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8405434.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
BASE COUNT          610 a          124 c          247 g          485 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatgga
//
LOCUS       DAYYAJ010000230         1437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000230 DAYYAJ010000000
VERSION     DAYYAJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.238"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..462
                     /locus_tag="ACHATZ_002988"
     CDS             52..462
                     /locus_tag="ACHATZ_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8405435.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            476..832
                     /locus_tag="ACHATZ_002989"
     CDS             476..832
                     /locus_tag="ACHATZ_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8405436.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          532 a          208 c          268 g          429 t
ORIGIN      
        1 tttaaaagaa attactgtag aattggatta taattaaaaa ggagtgagaa aatgataaaa
       61 gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat
      121 caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa
      181 aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt
      241 tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc
      301 aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc
      361 ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga
      421 aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga
      481 agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga
      541 acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatttaa gtacagaaga
      601 agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc
      661 tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt
      721 tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg atgaagttct
      781 atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac
      841 tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa
      901 gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat
      961 gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa
     1021 tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa
     1081 aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagttgtt
     1141 aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa agttcttgac
     1201 aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa aacgaaataa
     1261 aaaaaacttt taaaaaagag ttgacaatta cttgtctctc tgatatgata taaaagttgc
     1321 tacggcagcc aaaaacgaat cggggaacac tcctgatgaa gtagaccttt gaaaactgaa
     1381 caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca acaaaca
//
LOCUS       DAYYAJ010000231         1423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000231 DAYYAJ010000000
VERSION     DAYYAJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.239"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..1390
                     /locus_tag="ACHATZ_002990"
     CDS             98..1390
                     /locus_tag="ACHATZ_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFM8405437.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          257 g          411 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DAYYAJ010000232         1421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000232 DAYYAJ010000000
VERSION     DAYYAJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.240"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHATZ_002991"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHATZ_002991"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8405438.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          496 a          184 c          236 g          505 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta t
//
LOCUS       DAYYAJ010000233         1392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000233 DAYYAJ010000000
VERSION     DAYYAJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.241"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..328
                     /locus_tag="ACHATZ_002992"
     CDS             <1..328
                     /locus_tag="ACHATZ_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8405439.1"
                     /translation="KSFSMNNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVL
                     DKMVLSSSYQQIPRKKTVIEFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVE
                     QKNKKN"
     gene            373..1347
                     /locus_tag="ACHATZ_002993"
     CDS             373..1347
                     /locus_tag="ACHATZ_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8405440.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
BASE COUNT          559 a          129 c          249 g          455 t
ORIGIN      
        1 aaaaagtttt tctatgaata atttcgatgt tatagaagca tggcaatata acaaagagaa
       61 gattttagat gcttttccag atttgcatga ttcctattat cgccgactat gttgggcata
      121 tttttacgtg ttggataaaa tggttttatc aagtagttat caacaaattc cacgtaaaaa
      181 aacagtaatt gaatttttaa agaagaacag aaattttata ttaacttata agggattcac
      241 taaatcgcgc aaaatagcta ttattgctct gagctttaga gtagattggt ataggaaatt
      301 ggttgaacaa aaaaataaaa aaaattagaa tgtttaaatt agtaaataaa agaaaaggaa
      361 ttgaaaacta aaatgtgcag aataagtata atagttccag tttataatgt tgaaaaatat
      421 ttaaaaaagt gtgtcgattc aattctaggc caaacattta ccgattttga attattatta
      481 attgatgatg gttctacaga taattcagga agtatatgcg atgaacttgc gaaaacagac
      541 aaccgaataa aagtgataca taaggaaaat gggggtctaa gtgatgccag aaatattggt
      601 attgaggtag caaagggaga ttttattggg tttatagata gtgatgatta cattgatgaa
      661 gatatgtatg catttttata taacaatata ttgaaatatg atgcagagtt atcaatgtgt
      721 ggtatttatg acgtccataa gaataaagaa attaaaaagt taactccttt ttctcaattg
      781 gttactaaat ctgaagcaat tgaattagta ttagatggta agctagttgt tgctaatgca
      841 gtaagcaagc tatataaaaa agaattattt gagaatgtga gatatccaaa aggaaaaatt
      901 gctgaagacg cagcagtaat actgaaaatt atcaatcagt gtaataaaat ccatgttgat
      961 acttctcaaa aatattatta ttatcatcgt ggagatagta ttactagtaa aaaatttaca
     1021 aaaaaagatt ttgatataat tgatgtatgg aatgagaatg aaaaatggat tattaaaaat
     1081 tatcctcaaa tgtacgataa agtacatact agagtttgtt gggcttactt tgttgtgtta
     1141 gataaattag ttttaactga agaaaaagga ctgtatcaag aaaaaaaaca agtaagagag
     1201 tatttaattg ataatttctt ttttattata aaaaataaac gattaacttt tcaaagaaaa
     1261 atatctatgt tattgcttac ttttggaatt gctgcgtata aaataccggt taaatatcag
     1321 tataagaaag taaaggctgt taattaatgc tttagttagg atgaattaga tgaaaaaaag
     1381 caggttagag ta
//
LOCUS       DAYYAJ010000234         1376 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000234 DAYYAJ010000000
VERSION     DAYYAJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.242"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..>1376
                     /locus_tag="ACHATZ_002994"
     CDS             92..>1376
                     /locus_tag="ACHATZ_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8405441.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          467 a          268 c          257 g          384 t
ORIGIN      
        1 ccccattttc attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac
       61 aaaataaaca actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag
      121 aataacagag aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa
      181 gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc
      241 tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat
      301 tgtccgtttt gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta
      361 tacctgtaaa aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca
      421 ttcaatcgca aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt
      481 atcttttatt gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga
      541 gtttaaaagc tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga
      601 cgaatttcgt tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga
      661 aacaggaaaa ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt
      721 cttaggttgt accaatccag aagaagtaga attcttggtg acagacatga acgccgccta
      781 cttccagctc accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat
      841 tgtcaaacac atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg
      901 taaagccgga cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa
      961 aaatcgtgca aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa
     1021 atacccattt cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct
     1081 aaaggaggcg tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga
     1141 cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa
     1201 gcttcaaaac cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc
     1261 caatggaaaa atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg
     1321 atttaaatca tttgagaaca tgagaattag aatctttttg atcaatcaat taatca
//
LOCUS       DAYYAJ010000235         1374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000235 DAYYAJ010000000
VERSION     DAYYAJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.243"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACHATZ_002995"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACHATZ_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8405442.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAYYAJ010000236         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000236 DAYYAJ010000000
VERSION     DAYYAJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.244"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHATZ_002996"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHATZ_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8405443.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYAJ010000237         1334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000237 DAYYAJ010000000
VERSION     DAYYAJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.245"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHATZ_002997"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHATZ_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHATZ_002998"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHATZ_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          478 a          218 c          180 g          458 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aaga
//
LOCUS       DAYYAJ010000238         1300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000238 DAYYAJ010000000
VERSION     DAYYAJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.246"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1135)
                     /locus_tag="ACHATZ_002999"
     CDS             complement(92..1135)
                     /locus_tag="ACHATZ_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8405444.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          448 a          259 c          247 g          346 t
ORIGIN      
        1 ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca ttgttccatg
       61 cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa ataaatagta
      121 aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag cgtgtacctt
      181 ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca gtgatctcaa
      241 accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa aacaagctgt
      301 tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat tttcgttttt
      361 tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg aatttttcaa
      421 acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc atggcgaatt
      481 cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca gattggagtg
      541 taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt aatccaagaa
      601 aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg atctgtcctt
      661 tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa gaaccacaaa
      721 cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt tttttaccaa
      781 tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca attcctttta
      841 caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa acagagaatt
      901 ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt ttaaaaaaag
      961 tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac tgtggcagat
     1021 aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga
     1081 tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat ttcatttgat
     1141 cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac taaaccagta
     1201 ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg tttcgaataa
     1261 acggtgttcc agaagtagct tctacggaaa taagccgaaa
//
LOCUS       DAYYAJ010000239         1275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000239 DAYYAJ010000000
VERSION     DAYYAJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.247"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..97)
                     /locus_tag="ACHATZ_003000"
     tRNA            complement(26..97)
                     /locus_tag="ACHATZ_003000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Gln,seq:ttg)
     gene            complement(107..179)
                     /locus_tag="ACHATZ_003001"
     tRNA            complement(107..179)
                     /locus_tag="ACHATZ_003001"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:His,seq:gtg)
     gene            complement(241..325)
                     /locus_tag="ACHATZ_003002"
     tRNA            complement(241..325)
                     /locus_tag="ACHATZ_003002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Leu,seq:caa)
     gene            complement(346..416)
                     /locus_tag="ACHATZ_003003"
     tRNA            complement(346..416)
                     /locus_tag="ACHATZ_003003"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Cys,seq:gca)
     gene            complement(462..533)
                     /locus_tag="ACHATZ_003004"
     tRNA            complement(462..533)
                     /locus_tag="ACHATZ_003004"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Gln,seq:ttg)
     gene            complement(545..617)
                     /locus_tag="ACHATZ_003005"
     tRNA            complement(545..617)
                     /locus_tag="ACHATZ_003005"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(582..584),aa:His,seq:gtg)
     gene            complement(620..693)
                     /locus_tag="ACHATZ_003006"
     tRNA            complement(620..693)
                     /locus_tag="ACHATZ_003006"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Trp,seq:cca)
     gene            complement(700..780)
                     /locus_tag="ACHATZ_003007"
     tRNA            complement(700..780)
                     /locus_tag="ACHATZ_003007"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(744..746),aa:Tyr,seq:gta)
     gene            complement(782..857)
                     /locus_tag="ACHATZ_003008"
     tRNA            complement(782..857)
                     /locus_tag="ACHATZ_003008"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(822..824),aa:Phe,seq:gaa)
     gene            complement(893..965)
                     /locus_tag="ACHATZ_003009"
     tRNA            complement(893..965)
                     /locus_tag="ACHATZ_003009"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(930..932),aa:Asp,seq:gtc)
     gene            complement(975..1047)
                     /locus_tag="ACHATZ_003010"
     tRNA            complement(975..1047)
                     /locus_tag="ACHATZ_003010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1012..1014),aa:Val,seq:tac)
     gene            complement(1063..1134)
                     /locus_tag="ACHATZ_003011"
     tRNA            complement(1063..1134)
                     /locus_tag="ACHATZ_003011"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1099..1101),aa:Glu,seq:ttc)
     gene            complement(1149..1238)
                     /locus_tag="ACHATZ_003012"
     tRNA            complement(1149..1238)
                     /locus_tag="ACHATZ_003012"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1202..1204),aa:Ser,seq:gga)
BASE COUNT          364 a          333 c          292 g          286 t
ORIGIN      
        1 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
       61 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
      121 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
      181 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
      241 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
      301 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
      361 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
      421 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
      481 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
      541 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
      601 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
      661 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
      721 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
      781 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
      841 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
      901 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
      961 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
     1021 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
     1081 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
     1141 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
     1201 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
     1261 ctttctcaaa atgag
//
LOCUS       DAYYAJ010000240         1258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000240 DAYYAJ010000000
VERSION     DAYYAJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.248"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1015
                     /gene="scrK"
                     /locus_tag="ACHATZ_003013"
     CDS             143..1015
                     /gene="scrK"
                     /locus_tag="ACHATZ_003013"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8405445.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          388 a          225 c          289 g          356 t
ORIGIN      
        1 agaattttaa aaaatgataa aaattcaaaa agaaaaaata gtagttattt acttgtcatg
       61 ttactaaata aaaaaataat ttaaatatga tatatagtca tttatttagt gatttcattc
      121 taaaataaag ggagaaatgt ctatgctata cggtgcaatt gaagcaggcg gcacaaaatt
      181 tgtgtgtgcc attggcgatg aagaaatgac gatcaaggaa agagtgagct ttccgacaac
      241 gacacctgag gaaacaatgc ctttagtcat tgacttcttc aaacaatacc aagcagattt
      301 agcagggatt gggattggct catttgggcc tatcgatatc catcgcgatt ccgccactta
      361 tgggtacatt acctcgaccc cgaaattggc atggcaaaat tttgatttca tcggtaccat
      421 gaaaaaagaa tttccgatcc cgatttcttg gacaacagat gtcaatgcgg ctgcctatgg
      481 tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc gtttactata cgatcggtac
      541 gggtgttggc ggcggtgcct tacaagaagg gcgctttatc gaagggttta gtcatccaga
      601 aatggggcat atgctcgtcg tccctcatcc aaaagatgac tttgctggca gctgtccatt
      661 tcatggcaac tgtttagaag ggatggccgc tggacccgcc atcgaaaacc gcttagggaa
      721 aaaaggacaa gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc
      781 tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg
      841 tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa
      901 tgggtatgtc gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa
      961 cgctggaacg ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa
     1021 aaaaggctct tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa
     1081 ccagttaggc agagtgctta actggttttt gttttacttg gaagatcaag ccttgaatga
     1141 gataaattct tgctcgaaag ttatggaaat ttcgatagcc gtaggccact tgtattgtgc
     1201 aatagttttt tacaaaaaaa agggggtgcg gatatttttt tggacctttt taacaaat
//
LOCUS       DAYYAJ010000241         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000241 DAYYAJ010000000
VERSION     DAYYAJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.249"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHATZ_003014"
     CDS             200..742
                     /locus_tag="ACHATZ_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405446.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACHATZ_003015"
     CDS             898..>1246
                     /locus_tag="ACHATZ_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8405447.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAYYAJ010000242         1241 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000242 DAYYAJ010000000
VERSION     DAYYAJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.250"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..973
                     /locus_tag="ACHATZ_003016"
     CDS             122..973
                     /locus_tag="ACHATZ_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8405448.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            990..1235
                     /locus_tag="ACHATZ_003017"
     CDS             990..1235
                     /locus_tag="ACHATZ_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405449.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT          436 a          238 c          240 g          327 t
ORIGIN      
        1 ataccagaat caaaatcatt ttcttttaaa caaaaatttc aagagtagaa acaaaatgga
       61 ttcttttttt cgaaaaatgc gctacactta tagttgaacg aataaaaaag gagctgaaag
      121 tatgaattca ttaacaagca cttatcgtct atcaaatggt tacgaaatcc cggtagtagg
      181 attcggcaca tggcaaacac ctgatgggga tgtcgctgtt tcctctgtta aagaagcgct
      241 ggctgcaggt tatcgccata tcgatactgc ccaaggatac aagaatgaag aaagcgtcgg
      301 ccaagcgatt aaggaaagtg gaattcctag agaagagata ttcttgacaa caaaattatg
      361 gaatgcaaac cacagctatg aacttgtgat gtcttctttt gaagaatcat tgaagaagct
      421 ccaaacagat tatttagatc tgtttttgat ccactggcca aaccctgttg cttttcgtga
      481 taattgggaa caagcaaatg cagatacatg gcgagcattt gaagaactgt atgaagcagg
      541 taaaatcaaa gcaatcggtg tgagcaattt cttaccccac catctagata cactagcaaa
      601 aacagcaaaa attatgccga tggtcaatca agttttcctt gcaccaggcg aattacagac
      661 aactgtagtt gagtatgcaa aaaaacatga tatgatcctc gaagcatata gtccattagg
      721 cactggtaaa atcttcgatg tgcctgaaat gaaacaaatc gctgaagcac atgacaagac
      781 gattgcacaa gttgctttgc gttggtcgct gcaacacgaa ttccttcctc ttcctaagtc
      841 agtgacacca agcagaatca aagaaaatac tgaattattt gattttgaat taacagaaga
      901 agaaatgaaa caaatcgatc agttagatgg tgtagtcggc aaagcaaaag acccagatac
      961 tacccaattt taagtcgaaa ggtgatttta tggatccaat cgaacgcttg aattctctct
     1021 cggaagaagt tacccagact tttcattccg attttgtctt tttgatcgat gcagagaaaa
     1081 tccaacattt tcctgcaaga aactggacac atgatcaaat catcgaagaa ctgaaaaaac
     1141 ggttcgatca ttcgctaatg gtcaaaccct ggcacgaaca tgaagtcatt tattcacctg
     1201 aactacctgt ttttgccttg ataccgaaaa aatagaaaga a
//
LOCUS       DAYYAJ010000243         1240 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000243 DAYYAJ010000000
VERSION     DAYYAJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.251"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1240
                     /locus_tag="ACHATZ_003018"
     CDS             <1..>1240
                     /locus_tag="ACHATZ_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8405450.1"
                     /translation="VIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAELGIGSSII
                     YSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKNSTDIPHMY
                     RYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIALYFYSNFT
                     LFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIGNMSSKIGG
                     VIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGNFAVSNKKK
                     DSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQV
                     YRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGF
                     VIWYEAFIIYKTVFEKRFIHF"
BASE COUNT          407 a          154 c          196 g          483 t
ORIGIN      
        1 agtaatacaa tttgtaggta gaagtttttt tatttatttc ttaggagcga aatatttagg
       61 attaaatggt ttatttacaa atatacttag tttcttgtcg ttggctgaac taggcatagg
      121 ctcttctatt atttattccc tatacaaacc attagcccaa aaggatgaaa gtaaagtaaa
      181 ggctttaatg aatttgtata agaagacata tgaatttatt ggtattttta tcggcatagt
      241 aggagtattg attattccgc tgttacctct atttattaaa aatagcacag acataccaca
      301 tatgtatcga tattatgtat tatttttatt aaattcagtt cttagttatt ttttcacgta
      361 taaaagatca cttgcaatag cagatcaaaa aaattatctg gttgcgatta atgattttgc
      421 ctttttattc tttatgaaca ttgtacaagt aattgctttg tacttttatt ctaattttac
      481 tctatttcta atagtgcaga ttttgtttac tctattttct aatataaata tttccagaac
      541 agttgacaag agatattcat atttaaaaga gaaagatgta gtttctctag ataagcaaac
      601 aaaagatgaa gtaaagaaaa atgtcatagg taatatgtcc tcaaaaatag gcggggttat
      661 tgtaatgggg acagataata tattaatttc tacatttgtt aatttaacag ctgtaggaat
      721 ttattctaac tattcattga ttgtcacaag tgtgcaaaat ttatgtaagc aaattactaa
      781 ttctatcacg gcaagtgtag gaaattttgc tgtatcaaat aaaaaaaagg attcatttga
      841 gctatttaaa aaacactttt ttatcaacca ctcactaatc tttttttctt ctatattgtt
      901 attgacttta atgaatccct tcattagctt gtgggtagga gccaaataca tgctacctat
      961 gtcaacgact attttaattg ttttaaatta tagtgtacag gtttatagaa atacatcctt
     1021 cgtttttata gagtcatttg gtttatattg gtctcaacgg aaaaagccta taattgaagc
     1081 aggtgtaaac ttgttattat ctttgctttt gttaatagtt tttgatatgg gaatcaatgg
     1141 agtacttata gggactatcg gatcgtctct aggatttgtg atttggtatg aagcatttat
     1201 catatataaa acggtatttg agaaacgttt tattcatttc
//
LOCUS       DAYYAJ010000244         1214 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000244 DAYYAJ010000000
VERSION     DAYYAJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.252"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..606
                     /locus_tag="ACHATZ_003019"
     CDS             <1..606
                     /locus_tag="ACHATZ_003019"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8405451.1"
                     /translation="IYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNL
                     PVGWAIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTS
                     RSEGLPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNN
                     RLLMEKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            603..>1214
                     /locus_tag="ACHATZ_003020"
     CDS             603..>1214
                     /locus_tag="ACHATZ_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFM8405452.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLN"
BASE COUNT          436 a          106 c          225 g          447 t
ORIGIN      
        1 atttataacc cattgacatt aagcggctct agtttaataa gtaaccttga taaaaaaatt
       61 atttcattta ctgccagata cgatataaat attaaagggt ttgattattt atgtgaagta
      121 gctaaaaact tacctgttgg atgggctata aggattgctg gagatggtac agaagaagaa
      181 cgtaaagagc tgaaaagatt aattgagaaa aatggtgtgt tagataaaat tatttgcgca
      241 ggtgcattat ttggagatga attaatcaat cattatatag aaagttctat tttcatatct
      301 acttctagaa gtgagggact accattagtg ttgatagaag caatgactta tggattacct
      361 gtaataagtt ttgatcatgg tggtgctaag gaaatattac aagatggaaa ttatggaatt
      421 ttaattaaga attttgacgt acaagatatg aataatgaga tagaaaaatt gtctaataat
      481 cgtttactta tggaaaaata tcaaaaattc tctttagaaa gagctaagga ttttaatgaa
      541 aaaaaaataa ttggtgaatg gcaaacattg ttaaatgaat taggaaatga ggatgttaga
      601 aattgaggac ttcgattaaa aattttaatt tatggaaaga atggtatcta tatatatttt
      661 ttatagggat gattattcgt gcaataaatt tttatagttt attaccgtcc acttttgata
      721 gtattttttt taaacttata ggaatttcag gagttttgct attagggata gatttcataa
      781 ttaaatttaa taaaagaaaa gtaacttata acattttatt gatagcatat atggtgattt
      841 tagtaataac gagttttgtt cataaagaat atggtctagg agaaaactta aagactataa
      901 tgtgggcaat cttacagtat tttttgattt atcagtttgc ttttgagaat agcgataata
      961 aacgattttt taaaaggata agttggggat ttatttatag ttggtttgtt ttagtatcaa
     1021 tgtcaatggt tcttttcttt ggtaaattcg gctatgagaa atattataat gctagacata
     1081 ggatcagaat tggtttttta gaatcaagac tgtttgggac gttttctgat attaacaatg
     1141 ctgctatagc atctttagtt gttattgtcc taatcttatt ttatctttat aataaaaaag
     1201 tgacaggatt aaat
//
LOCUS       DAYYAJ010000245         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000245 DAYYAJ010000000
VERSION     DAYYAJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.253"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHATZ_003021"
     CDS             <1..1076
                     /locus_tag="ACHATZ_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8405453.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYAJ010000246         1065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000246 DAYYAJ010000000
VERSION     DAYYAJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.254"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>1065)
                     /locus_tag="ACHATZ_003022"
     CDS             complement(39..>1065)
                     /locus_tag="ACHATZ_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8405454.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          178 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaac
//
LOCUS       DAYYAJ010000247         1061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000247 DAYYAJ010000000
VERSION     DAYYAJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.255"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..938)
                     /locus_tag="ACHATZ_003023"
     CDS             complement(69..938)
                     /locus_tag="ACHATZ_003023"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8405455.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT          302 a          221 c          184 g          354 t
ORIGIN      
        1 aaaaagaatg gagagcgact tcaaaagtcg ttctccattc ctatatatcg tatctgttat
       61 gtggatgctt acgctttgtt agctgaaccg aaccagtcga tatgagattt agcatcttca
      121 acgattgctg ctttacctgg agctaataat ttacgagggt cgaatccttt accttcttta
      181 tctttaccag cttcgatata tttacgagta gctgcagcaa atgataattg gaattcagtg
      241 ttaacattta cttttgagat acccattgag atagcttttt cgatttgatc ttgaggaata
      301 cctgatccgc catgtaatac taaaggcatg tctggaccta cagcatcagc aattgcttgt
      361 aagtggtcaa aagctaatcc tttccagttt tctggatatt gaccatggat gttaccgata
      421 ccgcaagcta agtagtcgat acctgtagca accatttgtt tgcattcttc aatatcagct
      481 aattcgccgt taccgatgat tccgtcttct tctccgccga ttgaaccaac ttcacattca
      541 actgagatac cttttgcatg tgctttttca acaacgtctt tagctaattt caagttttct
      601 tcaaatggta gatgtgaacc atcgaacatt actgaagtat agcctaattc gatacattcc
      661 aaagcagctt cgtaatcacc atggtctaag tggatagcaa ctggaacagt gatgttcatt
      721 gcttcaacta aatcagtgat taaatcttta cagattttgt aaccacccat gtatttagct
      781 gcacccattg atgtttggat aagtactggt gcttgttttg cttcagccgc ttcaaggatt
      841 gcttgagtcc attccaagtt gtttgtgttg aatccgccga cagcgtaacc gcctttacga
      901 gcagctttta aaaattcagc tcctgatact actggcataa taaaattcct cctaagtttt
      961 gtaaaactaa atttgttaag acaattctta accaacgacc ctattttacc aaacatttcg
     1021 gtagttttct actaaaatca atgaaaatga aaattttcaa g
//
LOCUS       DAYYAJ010000248         1061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000248 DAYYAJ010000000
VERSION     DAYYAJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.256"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1038
                     /locus_tag="ACHATZ_003024"
     CDS             19..1038
                     /locus_tag="ACHATZ_003024"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8405456.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          356 a          172 c          249 g          284 t
ORIGIN      
        1 ttatttggag gaatatccat gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc
       61 actgctttag cacaaacatt ggcagaaaac gggcatgatg tccgtatttg gggaaatgta
      121 cctgaacaga ttgatgaaat caataagtat catacaaatc agcatttttt acctgatttg
      181 acgatcccag aatcgattat cggatataaa gaattggcag aagcagtgga taacgcagat
      241 gctattttat ttgttgttcc aacaaaagcg attcgttcag tcgcacagga acttattggt
      301 aaaatgaata ctaagccaat cattatacat gcaagtaaag gactagaaca agatacgcat
      361 aaacgaatct cagaagtttt ggctgaagaa attccagaag aaaaaagaca ggcaattgtt
      421 gtattatctg gtcctagcca tgctgaggaa gtggcagtac atgatatcac aacaattaca
      481 gctgcaagta ttgatcaaaa agcagcagcc tatgtacaaa aactgttcat gaatgattat
      541 ttcaggattt atacaaataa tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat
      601 atcattgcaa ttggtgccgg cgctatccat ggattaggat tcggagatga tgcaaaagca
      661 gcaatcatga ctcgaggatt ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat
      721 cctctgacat ttatcggttt gagtggtgtg ggggacttga tcgttacttg tacaagtgtt
      781 cattctcgca attggcgtgc cggtaacttg ttaggacagg gacataagct ggaagaagtt
      841 ctagaaaaca tgggaatggt cgttgagggt gtggcaacga caaaagcagc agtagagcta
      901 gctcaacagc taggtgttga gatgccaatt actcaaacaa tctataatgt tttgtataat
      961 ggagaagata tcaaaaaagc agcaaaagaa atcatgttac gtgacggaaa aatggagaat
     1021 gaattttctc ttcgttaaat agatgatcaa tagttaatcg t
//
LOCUS       DAYYAJ010000249         1026 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000249 DAYYAJ010000000
VERSION     DAYYAJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.257"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1024
                     /locus_tag="ACHATZ_003025"
     CDS             <1..1024
                     /locus_tag="ACHATZ_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8405457.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHG
                     KTFLTK"
BASE COUNT          320 a          209 c          195 g          302 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca accacatcaa agtattaaaa cgcaatgcgt acggctttcg
      961 caacttttac aatttcaaac tacgaattat gattcgtcat ggcaaaacat ttttaaccaa
     1021 ataaac
//
LOCUS       DAYYAJ010000250         1020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000250 DAYYAJ010000000
VERSION     DAYYAJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.258"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHATZ_003026"
     CDS             <1..459
                     /locus_tag="ACHATZ_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8405458.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            468..695
                     /locus_tag="ACHATZ_003027"
     CDS             468..695
                     /locus_tag="ACHATZ_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405459.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          318 a          166 c          180 g          356 t
ORIGIN      
        1 ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt
       61 ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta
      121 tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg
      181 aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta
      241 aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa
      301 tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc
      361 gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat
      421 gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg
      481 ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag
      541 tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt
      601 tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct
      661 ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata
      721 aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca
      781 gattttttat ttcgattgaa taccgtttta tctataaata agatatgtac tgactcatac
      841 aaagggggcc ttttcttctc catgtactat cacttttcct gatacaactg gtttcctgtt
      901 acgttgtagt tggatctgct tttagtataa tactttattc attccaccag actgatagca
      961 gtttgagcag tctaaataat catgtagtat tacaggccat tctgtttcat accttgaagt
//
LOCUS       DAYYAJ010000251          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000251 DAYYAJ010000000
VERSION     DAYYAJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.259"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHATZ_003028"
     CDS             <1..>986
                     /locus_tag="ACHATZ_003028"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8405460.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYAJ010000252          984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000252 DAYYAJ010000000
VERSION     DAYYAJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.260"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHATZ_003029"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHATZ_003029"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8405461.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          257 a          221 c          152 g          354 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt ctttcgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atca
//
LOCUS       DAYYAJ010000253          983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000253 DAYYAJ010000000
VERSION     DAYYAJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.261"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..644
                     /locus_tag="ACHATZ_003030"
     CDS             <1..644
                     /locus_tag="ACHATZ_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8405462.1"
                     /translation="VIFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKISI
                     LNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQ
                     NEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNN
                     IQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          434 a          117 c          141 g          291 t
ORIGIN      
        1 tagtaatttt ctccggaaag caatatgctg ataatatttt attatgggga ggggaagata
       61 atattgaaaa gatcaacaac aatttaacta tattagacac agctctagaa aataaagagc
      121 aaaaaattag tattttaaat tcacaattgt cttcaaacaa ccaaactctt gaacaactaa
      181 gaactaatat tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac aagaatcaat
      241 tagtatcaga gaaaactaat ttagaaaatc aattgtcagc aaaagactct gaattgcaag
      301 gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta gctgaaataa
      361 atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa tttaatgaag
      421 aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt gaaaaaaata
      481 acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag tcaaaaaata
      541 gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta gaggataccg
      601 caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag ttttaattta
      661 acaaatggta tgtttggaga taatgagata gaatataaat gtcataatat tatacaatct
      721 aaacaccgtt tccttttcta tttttgatga catcagtata tagctactcg aaagagtagt
      781 ctttttatta ttctcgctta ttattgatat gaaaatttcg atttgacaaa aggtaccgaa
      841 taattttgta atatgaatta ttatcaagaa gtaattatga tgataacata tggaaactag
      901 ttagtaataa taacaggtga ttaaactttt gaacgacttg taattctata ttttttcttt
      961 taagtaaaaa atataggtga ttt
//
LOCUS       DAYYAJ010000254          954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000254 DAYYAJ010000000
VERSION     DAYYAJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.262"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>954
                     /locus_tag="ACHATZ_003031"
     rRNA            <1..>954
                     /locus_tag="ACHATZ_003031"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          266 a          212 c          288 g          188 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttg
//
LOCUS       DAYYAJ010000255          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000255 DAYYAJ010000000
VERSION     DAYYAJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.263"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHATZ_003032"
     rRNA            <1..>943
                     /locus_tag="ACHATZ_003032"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYAJ010000256          819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000256 DAYYAJ010000000
VERSION     DAYYAJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.264"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>819
                     /locus_tag="ACHATZ_003033"
     rRNA            <1..>819
                     /locus_tag="ACHATZ_003033"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          205 a          182 c          250 g          182 t
ORIGIN      
        1 gttttcggat cgtaaaactc tgttgttaga gaagaacaag gatgagagta actgttcatc
       61 ccttgacggt atctaaccag aaagccacgg ctaactacgt gccagcagcc gcggtaatac
      121 gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc ggtttcttaa
      181 gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat tggaaactgg gagacttgag
      241 tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa
      301 caccagtggc gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg
      361 agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg
      421 agggtttccg cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga
      481 ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt
      541 ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag
      601 atagagcttc cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt
      661 cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt
      721 cagttgggca ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca
      781 aatcatcatg ccccttatga cctgggctac acacgtgct
//
LOCUS       DAYYAJ010000257          763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000257 DAYYAJ010000000
VERSION     DAYYAJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.265"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..265
                     /locus_tag="ACHATZ_003034"
     CDS             2..265
                     /locus_tag="ACHATZ_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8405463.1"
                     /translation="MWIFDRSRNASIGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLS
                     STLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(356..721)
                     /locus_tag="ACHATZ_003035"
     CDS             complement(356..721)
                     /locus_tag="ACHATZ_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405464.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          286 a           99 c          138 g          240 t
ORIGIN      
        1 tatatggatt tttgatagga gtagaaatgc aagtataggt tttagaacta aacgaagtct
       61 gtcttctaaa aaaaattggg tatactcaca aactattttt tatggaggaa ttgttttaat
      121 tagtttactt tcttcaacac tttattcttt gaatattata gatgtttcca cctcaaattc
      181 aatatctatt attggaatca ttattgctgc aattattaca caactgtttt tagtgtttgg
      241 agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag ttttggtgat ataagaaaaa
      301 catatatgta taaaaagaag aaagtagctt ctctttgtac atatatggtt tgtttttatg
      361 gatgccgagc ttttttataa gataaggcta ccgcccataa cgacccaagg atttgtggaa
      421 ctaagtaaat acctgcaaga agcaagatca gctgggtcgg ttttaaagga tgatagaagt
      481 aacgccattc agtaaaattg gcgcaaaata aataggaagt gacagtagat ataatattgg
      541 caatgaataa agatataata cgcctgttcc tttgaaaata atatccagca aaaagagata
      601 aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg aaaatcaagc agtaaggcga
      661 aatgcacata aggaacaaaa gataaaagca acggaaggtt tttccagcaa gtttttatca
      721 tctttcatca ccacctaaca agtgttatta ttttgttagt ttt
//
LOCUS       DAYYAJ010000258          756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000258 DAYYAJ010000000
VERSION     DAYYAJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.266"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..>756
                     /locus_tag="ACHATZ_003036"
     CDS             201..>756
                     /locus_tag="ACHATZ_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8405465.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNA"
BASE COUNT          303 a          131 c           91 g          231 t
ORIGIN      
        1 tttttccgcc ttaaaaacaa gaatatcttg acaaaatacc ctaggaaata aacaatagaa
       61 taaacaaggt aacaaaaaaa ttaaataacg ttatataaaa aagaaggcga cactctgtga
      121 gaataacaca gactgaaatg actttccact cacagttttt gtctaactat atacaaaaac
      181 agaataaaaa ggaggtattt atgctaattg aagcaagaaa aaaagagtta cttgttctac
      241 aaatggtcat tcaaaaaaat aattgttctt tacaagaatt atccaatgac ttacatattc
      301 caaaacggac aatcaaagaa ctgatccgaa aaataaatgt atcactcaag caatttttat
      361 tgattgatga atttatttat tctaatcata aaggagagat ccaaaccaaa gaaaccgatc
      421 aaactaaaaa gttagctgtt ttttctaagt taaaattatg ctacctaaaa gaatccaatc
      481 gttttaacta tttgatgtta ctaatcaatt atccacaaac tgctgtacca aaaaaatatt
      541 tacttgaaca actttatata tcgccctcct atctcgaaaa actgacaaaa cagctcaatc
      601 attccttaat aaaattccat ttacggatcc tatctgtaaa aaattgctgt actcttgaag
      661 gaaatgagtt atctatccgt ctctatcttt acttttttct ttcagataca tttggaggta
      721 acgattggcc attttcctca cttgaattaa atgcac
//
LOCUS       DAYYAJ010000259          726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000259 DAYYAJ010000000
VERSION     DAYYAJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.267"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHATZ_003037"
     CDS             complement(<1..613)
                     /locus_tag="ACHATZ_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8405466.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          212 a          138 c          142 g          234 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gatttt
//
LOCUS       DAYYAJ010000260          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000260 DAYYAJ010000000
VERSION     DAYYAJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.268"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHATZ_003038"
     CDS             complement(54..629)
                     /locus_tag="ACHATZ_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8405467.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYAJ010000261          675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000261 DAYYAJ010000000
VERSION     DAYYAJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.269"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACHATZ_003039"
     CDS             complement(<1..447)
                     /locus_tag="ACHATZ_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8405468.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          200 a          135 c          103 g          237 t
ORIGIN      
        1 agacacttgc ttaaagcgaa aaatttcttc aatgtccatg cctaattctt tggatattct
       61 cttgtctgac catccatttt ttactaatct ttggatgaca atttccattc ctcttatttc
      121 atgggtccct cttgcacgat tgtgtcgaat tgttgaagcc atacgttctt ctatgggttt
      181 atcaataact gttaccggta caacttttaa gttgagtctt tccttagcaa cacgatacct
      241 atgaagcaca tcaaccaaga tatacttctc ttctttttcc ttgtaataac aaactaatgg
      301 ttgagtaaag ccatcttcaa tgatagaaag ctccaataag ttcatttccg gagacgctac
      361 tcgattggga ttataatcgt tcgcctgtat tttggtaatt ggaacaagcc gaacgtccat
      421 cacagggaaa gttatgtctt tattcatacg cttgtatacc ctactttctt aaaaagtagg
      481 gtatactcaa gaatccgatt aattatcttc tcaatttgga taatatatct catcagtctt
      541 cttgtcactt tatcaacatg attttgctgc aaaatgggat accgacagta ctggactggt
      601 aatcaggata aaagcaaaaa taattaattt gtatcctaaa aaatccataa aaattctctc
      661 acattttcgg gtatt
//
LOCUS       DAYYAJ010000262          646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000262 DAYYAJ010000000
VERSION     DAYYAJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.270"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..249
                     /locus_tag="ACHATZ_003040"
     CDS             <1..249
                     /locus_tag="ACHATZ_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8405469.1"
                     /translation="SVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFK
                     VSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            321..>646
                     /locus_tag="ACHATZ_003041"
     CDS             321..>646
                     /locus_tag="ACHATZ_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405470.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNV"
BASE COUNT          277 a           85 c           99 g          185 t
ORIGIN      
        1 tcggttccat ttgaatttga cgttcatgcg atgatttttt cagaggacgc tccgtctttg
       61 gaaactaaat tgcataatca ttttagaaaa caagaagtta ataaaattaa tcaaagaaaa
      121 gaatttttca aagtttcact agacgaaata gagaaagttg ttttagaaaa ttataatgga
      181 acagtaacat ttacaaaatt agctaaagct gaacaatata gacgatcttt agaattgtca
      241 aaagattaaa tatataaaaa gccttcgggc tttttctttt aacaattaat aggaacgtat
      301 attcgaaagg aatagaaata atgtggttag aaaaaataaa tgacaaaaaa tttaagtaca
      361 ccgaacgtta tacggatccc ttaactgaaa aaaagagaaa agtgtccgtt actttatcta
      421 gcaattcacg ccaagcttgg aatcaagcta acttactttt gaatgaaaaa atcgcagaaa
      481 aaataaaacg aaatgaagaa ttaccactaa cttttggaga attaaaaact aaatgggatg
      541 aaaaatacaa gccaactgta aaagaaagtt cctatcgaac aactcaggtt tatctatcat
      601 taatttcaaa atatataaaa gatgatgtac ttgtaaaaaa cgttaa
//
LOCUS       DAYYAJ010000263          625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000263 DAYYAJ010000000
VERSION     DAYYAJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.271"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..625
                     /locus_tag="ACHATZ_003042"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACHATZ_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DAYYAJ010000264          579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000264 DAYYAJ010000000
VERSION     DAYYAJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.272"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..>579
                     /locus_tag="ACHATZ_003043"
     CDS             156..>579
                     /locus_tag="ACHATZ_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405471.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTK"
BASE COUNT          216 a           93 c           98 g          172 t
ORIGIN      
        1 aacaatttgt ggtatgatta tatagggaaa acttgtttac aaatatttct gtgaatatat
       61 taatttaatt atgtttttta aaataaaaag ctgtcatttt tatgtatgtg aattatatct
      121 taatttatct atcatcaata cttaggagtg aatctatgga aaagccattc gtcaagtcat
      181 tgactgctgt ggcggtaggt gcaggtgcta ttgcgatctg tttgtttggt taccatatca
      241 ataatcagcg gcaacatcag caacggatca attatgcaga atctgccatc accaatcaaa
      301 aagacacctt gacttcttta tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa
      361 attttctaaa ccctgaaata acagaagaaa caatcacaag tttgtcaaat aagctatcct
      421 ctgtcaaact ttcagcagat gattacgaca tcaaagaatc agaattgcct aaagaagctg
      481 ttgcaatcca agatgagaag aaagcaatcg tgacacagtt agatagtgca gaatcaaaac
      541 tgaagatcca gacagctgtc aacgatttat ttactaaaa
//
LOCUS       DAYYAJ010000265          544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000265 DAYYAJ010000000
VERSION     DAYYAJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.273"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..445)
                     /locus_tag="ACHATZ_003044"
     CDS             complement(146..445)
                     /locus_tag="ACHATZ_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8405472.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          178 a          100 c           64 g          202 t
ORIGIN      
        1 taattaaagg ataaaataca atataaagaa acatctgttt ctttatttag aataaaaaaa
       61 tacagactct gttcaaataa atgaacctag cctgcattat gagattgact atacttttta
      121 attcattgag atgaataact cgtttttata aatgcccaaa agtaatcggt gccgaagcca
      181 ttcctcgacc taattctttt tcaaattttc ctattgttga tatctccata taaaacggtg
      241 atactggttt agacaattct cccctgattg ctaacggacg taaactacgt tccaaattat
      301 ttatgacttt aggataatac tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta
      361 tacgttcctt ttctttgatc tctggattta aaacgagatc atataatttt agtaccatgt
      421 tttctatttc ttcactttta gacatcacga tttcctccca aaaatatatt tcttcccact
      481 acattctata aacaaagtat acgctacatc tagatttttt taaatcatta ctggattgtt
      541 aaaa
//
LOCUS       DAYYAJ010000266          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000266 DAYYAJ010000000
VERSION     DAYYAJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.274"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..>526)
                     /locus_tag="ACHATZ_003045"
     CDS             complement(140..>526)
                     /locus_tag="ACHATZ_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8405473.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           75 c           97 g          196 t
ORIGIN      
        1 taataaaaaa gtgcgaaatg ccgcactctt tatttagttt ttatgcaaaa aaacaagtta
       61 taagataaag taggtgggat tttgttaggg agcgtcaata attttgtgta aataaattgt
      121 cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg gttacctgtt
      181 gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa atgctgacta
      241 gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg tatcttttaa
      301 tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga atgctaggtg
      361 gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc actttaggat
      421 agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct tcttttgagt
      481 tagcttgata aacagcctta aagtcatcac agatttcttt tcggtc
//
LOCUS       DAYYAJ010000267          522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000267 DAYYAJ010000000
VERSION     DAYYAJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.275"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          179 a           98 c           59 g          186 t
ORIGIN      
        1 taatatataa cacagttatt tttaaaaata agaatgctaa atcactctac aataacggcg
       61 aaagcacaaa aaataaaaaa attattacgt tattaaaaaa acagatgaaa gatcaggttt
      121 tattagatct cattctttca tcacttgaag ggatttaatg aactactttt tcctctttgt
      181 cgtttttata tattcgtatt ccttcaaaca gtaggatagc ctctttcaat gcttgatatt
      241 tgaactgtcc gttacttaaa aatttagatg tgccgccctc ttcctagaac atattctttt
      301 ttactttttc attaattgac agaccaatcg aacgaaactg taaatttcga taggataatg
      361 attggtcaaa ctcattcatt ttcaagacat ctcgattcat attcaagata ggaatctttt
      421 tatcaacatc actttattcg tattttcctc taccataaaa gcttcacgct ccacaaaatt
      481 gttttataca ctactattca gcccattcaa cgaccaatga at
//
LOCUS       DAYYAJ010000268          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000268 DAYYAJ010000000
VERSION     DAYYAJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.276"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHATZ_003046"
     CDS             complement(<1..132)
                     /locus_tag="ACHATZ_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8405474.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAYYAJ010000269          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000269 DAYYAJ010000000
VERSION     DAYYAJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.278"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHATZ_003047"
     CDS             <1..>468
                     /locus_tag="ACHATZ_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8405475.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAYYAJ010000270          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000270 DAYYAJ010000000
VERSION     DAYYAJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.279"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHATZ_003048"
     CDS             <1..>451
                     /locus_tag="ACHATZ_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8405476.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYAJ010000271          435 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000271 DAYYAJ010000000
VERSION     DAYYAJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.280"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACHATZ_003049"
     CDS             complement(<1..190)
                     /locus_tag="ACHATZ_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405477.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLP"
BASE COUNT          143 a           79 c           48 g          165 t
ORIGIN      
        1 tagggagcca ttttttgatt ttcactaatt gattgctcat aaatctttca ctggactttt
       61 gttcaatctt aaattgcaaa acttctaact cataattccc atcttctgat ttatttttgg
      121 gaacaactat caaactttta taaagtttat ccacatcaaa atttatcttt tttgcataat
      181 ttgtagccat taatacatct ccttattcat aataaatttc atatgacgct cctctcaaaa
      241 tttaccggta gactagaaaa aagaataaaa gatacgtcaa ttcacacttt attcttaaaa
      301 aaaaactggt ctctctaccg gtaatatcga ttgagtccgc ctttatcgac tttatccttt
      361 ttttatatta aattttttca ccggtagaca gggagagaaa gaataaacta aaaagccctt
      421 aattcttaat gtttt
//
LOCUS       DAYYAJ010000272          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000272 DAYYAJ010000000
VERSION     DAYYAJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.281"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHATZ_003050"
     CDS             complement(109..>414)
                     /locus_tag="ACHATZ_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8405478.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAYYAJ010000273          389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000273 DAYYAJ010000000
VERSION     DAYYAJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.283"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          140 a           63 c           64 g          122 t
ORIGIN      
        1 tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttacaatt
       61 ggagtggtag ttaattagtg acttattttt gattttatag cactgatact ataaaatata
      121 gatatcatca tattacacaa tcttaatact aacttaaaaa atatctcctt tcacaagtat
      181 ggtgataaaa ttcgttccgg gctacctttt taggtagcct actttaatct ttataccttt
      241 ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgcta cgtgcgtccg
      301 gcacgtcagt catgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtagaata
      361 ataaaaaaca cactggagga tttttcatg
//
LOCUS       DAYYAJ010000274          384 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000274 DAYYAJ010000000
VERSION     DAYYAJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.284"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>384
                     /locus_tag="ACHATZ_003051"
     CDS             <1..>384
                     /locus_tag="ACHATZ_003051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405479.1"
                     /translation="NMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTINNIVDL
                     EAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNAEKKVI
                     LDKCHFGLNLFKSNTIIGLSLKSIE"
BASE COUNT          155 a           40 c           64 g          125 t
ORIGIN      
        1 aaaatatgac catttatcca tgttctttca atgtagatga ccaaaatgat tatggagaga
       61 aaatagtcaa taaaagctta gagtctacgt tgaaccttgc ttatttaggt acgattaata
      121 atatcgtaga tttagaagcg attaatgaat tattgaaaac atgcaaaaga aaatttggaa
      181 acaaaataaa tctttacttt attggaaaag gtgaaagcaa aactaaatta gctgatattt
      241 gtgaaaaaaa tcatattgaa tttgttgact gcggtgttat atatgacaat gcagaaaaaa
      301 aagttatact cgataaatgt cattttggac taaatttatt taagtcaaat accataattg
      361 gtttaagtct gaaatcgatt gagt
//
LOCUS       DAYYAJ010000275          379 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000275 DAYYAJ010000000
VERSION     DAYYAJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.285"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>379
                     /locus_tag="ACHATZ_003052"
     CDS             <1..>379
                     /locus_tag="ACHATZ_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8405480.1"
                     /translation="KIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKE
                     QQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYAN
                     KIQELEEKLKELEVIRENVLQREL"
BASE COUNT          183 a           37 c           62 g           97 t
ORIGIN      
        1 aaaaatattt gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg
       61 tcttttagag tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac
      121 cgaagaaaaa gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg
      181 tagagaaatt gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga
      241 aatttccaaa ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata
      301 tgcaaataaa attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa
      361 tgtattacaa agagaatta
//
LOCUS       DAYYAJ010000276          377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000276 DAYYAJ010000000
VERSION     DAYYAJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.286"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..363
                     /locus_tag="ACHATZ_003053"
     rRNA            <1..363
                     /locus_tag="ACHATZ_003053"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          103 a           72 c          115 g           87 t
ORIGIN      
        1 cccaagggtt gggctgttcg cccattaaag cggcacgcga gctgggttca gaacgtcgtg
       61 agacagttcg gtccctatcc gtcgcgggcg ttggaaattt gagaggagct gtccttagta
      121 cgagaggacc gggatggact taccgctggt gtaccagttg ttctgccaag ggcattgctg
      181 ggtagctatg tagggaaggg ataaacgctg aaagcatcta agtgtgaagc ccacctcaag
      241 atgagatttc ccatttcttt aagaaagtaa gatccctgag agatgatcag gtagataggt
      301 caggagtgga agtacagtga tgtatggagc ggactgatac taatcgatcg aggacttaac
      361 caataggtta aaagaaa
//
LOCUS       DAYYAJ010000277          375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000277 DAYYAJ010000000
VERSION     DAYYAJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.287"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>375
                     /locus_tag="ACHATZ_003054"
     CDS             82..>375
                     /locus_tag="ACHATZ_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8405481.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT          133 a           62 c           76 g          104 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccat
//
LOCUS       DAYYAJ010000278          375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000278 DAYYAJ010000000
VERSION     DAYYAJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.288"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>375)
                     /locus_tag="ACHATZ_003055"
     CDS             complement(<1..>375)
                     /locus_tag="ACHATZ_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405482.1"
                     /translation="PYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSAYSLYQANME
                     EEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTFTPQKVG
                     VNYPKRLTIIQYPQSTTGACQID"
BASE COUNT          100 a           73 c           80 g          122 t
ORIGIN      
        1 gtcaatttga catgcacctg ttgttgattg gggatactgg ataatagtta accgtttggg
       61 gtagttaact ccaaccttct gcggtgtaaa agtcagaccc catgtatcaa ctaacccttc
      121 tactggctct aagttaccat atctataatc tccactatcc tcgttgtaaa caatgaacgt
      181 ttggataggt ggtttggttg ccttcatggt aattgtatag gtttgaccta aaacaaattc
      241 ttcttccatg ttagcttggt ataggctgta ggcgctagat ttgattggaa acgtaacaga
      301 cttattagca atattctcac ccaaaggcgc tttacctagc cagtaagggt catcaagtaa
      361 gtttggctga tatgg
//
LOCUS       DAYYAJ010000279          343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000279 DAYYAJ010000000
VERSION     DAYYAJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.289"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>343
                     /locus_tag="ACHATZ_003056"
     CDS             <1..>343
                     /locus_tag="ACHATZ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFM8405483.1"
                     /translation="ESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPILIDQASE
                     HRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITKTSPYNKT
                     DEENFHLIVTTL"
BASE COUNT          138 a           48 c           47 g          110 t
ORIGIN      
        1 cgaatctatt gattctacaa tcgatagatt aaaaaaagaa attcgatttt ataatagagt
       61 aattggtatc ggaaatatta taaaaatagt cttatcagct agtatcccaa tattaattga
      121 tcaggcttcc gaacacaggt ctttactatt gattgtttca attgcttcag caataattac
      181 aattatacaa agtgggatgt ctgcatttaa ttatcaagat aaagtacaga cttcgacaga
      241 gttattaatg aaaattgaaa aagaaaagtt gttatatata acgaaaacat ccccatataa
      301 taaaacagat gaagaaaatt tccatttaat cgttacaacc ctt
//
LOCUS       DAYYAJ010000280          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000280 DAYYAJ010000000
VERSION     DAYYAJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.290"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHATZ_003057"
     CDS             complement(<1..>321)
                     /locus_tag="ACHATZ_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8405484.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYAJ010000281          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000281 DAYYAJ010000000
VERSION     DAYYAJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.292"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHATZ_003058"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHATZ_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYAJ010000282          307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000282 DAYYAJ010000000
VERSION     DAYYAJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.293"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHATZ_003059"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHATZ_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8405485.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAYYAJ010000283          300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000283 DAYYAJ010000000
VERSION     DAYYAJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.294"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..>300
                     /locus_tag="ACHATZ_003060"
     CDS             80..>300
                     /locus_tag="ACHATZ_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405486.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFL"
BASE COUNT          119 a           40 c           36 g          105 t
ORIGIN      
        1 aaatttaacg caaaacttat taaaaaaaga caattctagt atactgaaaa tagaattaaa
       61 aaaggagaac aacaaatcta tgaaaataag taaaaaagca tttcttttag tagttcttat
      121 cttacttagt actttatatt cagtaaattt tatgagaaat gctcaagaaa tttatacaac
      181 aggtgattta tcttttcatc tttcacgaat caaagggtta tcttctattt ttgaagggcc
      241 aatcaactat acaacattta acaattatgg agatggactg aactattttt atccttttct
//
LOCUS       DAYYAJ010000284          287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000284 DAYYAJ010000000
VERSION     DAYYAJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.296"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          117 a           32 c           50 g           88 t
ORIGIN      
        1 ttgttctaaa tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac
       61 ttttgatgtc atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt
      121 ttatcgaaaa aaacttcgaa aaagttattg acaaataaca ataaagatgc tatgataatc
      181 aagtcgctgc taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca
      241 ttctcttctg aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DAYYAJ010000285          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000285 DAYYAJ010000000
VERSION     DAYYAJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.297"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHATZ_003061"
     CDS             <1..>282
                     /locus_tag="ACHATZ_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8405487.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYAJ010000286          247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000286 DAYYAJ010000000
VERSION     DAYYAJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.299"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>247
                     /locus_tag="ACHATZ_003062"
     CDS             <1..>247
                     /locus_tag="ACHATZ_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8405488.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTF"
BASE COUNT           80 a           51 c           61 g           55 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 accttta
//
LOCUS       DAYYAJ010000287          244 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000287 DAYYAJ010000000
VERSION     DAYYAJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.300"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>244)
                     /locus_tag="ACHATZ_003063"
     rRNA            complement(<1..>244)
                     /locus_tag="ACHATZ_003063"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           52 a           81 c           49 g           62 t
ORIGIN      
        1 ttggacagac atttccgatc gtctgcattc cttagcctcc tgcgtccctc cattgctcaa
       61 acaaaaacaa cgggtacagg aatatcaacc tgttatccat cgcctacgcc tgtcggcctc
      121 ggcttaggtc ccgactaacc ctgggcggac gagccttccc caggaaacct tagtcattcg
      181 gtggacagga ttctcacctg tctttcgcta ctcataccgg cattctcact tctaagcgct
      241 ccag
//
LOCUS       DAYYAJ010000288          235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000288 DAYYAJ010000000
VERSION     DAYYAJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.301"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>235)
                     /locus_tag="ACHATZ_003064"
     CDS             complement(<1..>235)
                     /locus_tag="ACHATZ_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8405489.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           69 a           45 c           36 g           85 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggc
//
LOCUS       DAYYAJ010000289          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000289 DAYYAJ010000000
VERSION     DAYYAJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.302"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAYYAJ010000290          225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000290 DAYYAJ010000000
VERSION     DAYYAJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.303"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>225
                     /locus_tag="ACHATZ_003065"
     CDS             22..>225
                     /locus_tag="ACHATZ_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8405490.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRT"
BASE COUNT           83 a           43 c           43 g           56 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaacc
//
LOCUS       DAYYAJ010000291          224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000291 DAYYAJ010000000
VERSION     DAYYAJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.304"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACHATZ_003066"
     CDS             <1..>224
                     /locus_tag="ACHATZ_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8405491.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAYYAJ010000292          220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000292 DAYYAJ010000000
VERSION     DAYYAJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.305"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACHATZ_003067"
     rRNA            complement(<1..175)
                     /locus_tag="ACHATZ_003067"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAYYAJ010000293          215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000293 DAYYAJ010000000
VERSION     DAYYAJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.denovo.307"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>215)
                     /locus_tag="ACHATZ_003068"
     CDS             complement(<1..>215)
                     /locus_tag="ACHATZ_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8405492.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQL"
BASE COUNT           68 a           37 c           35 g           75 t
ORIGIN      
        1 tccaattgtt gtaaggtttc aaatgtgtat tgatagacaa attctacttt caacgactta
       61 taagttgatt cagctacggc attatcaaaa ggacagcctt tatgactcaa tgatcgattg
      121 atgtcaaaag ttgttaataa ttcatcaata gcttggttat caaactcttt tccacgatca
      181 gtatgaaaaa tctcaacctc tgtcagaggt tgttt
//
LOCUS       DAYYAJ010000294         5792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000294 DAYYAJ010000000
VERSION     DAYYAJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.156"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1217)
                     /locus_tag="ACHATZ_003069"
     CDS             complement(<1..1217)
                     /locus_tag="ACHATZ_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8405493.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVA"
     gene            1496..2302
                     /locus_tag="ACHATZ_003070"
     CDS             1496..2302
                     /locus_tag="ACHATZ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8405494.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2320..3027
                     /locus_tag="ACHATZ_003071"
     CDS             2320..3027
                     /locus_tag="ACHATZ_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8405495.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            3024..3995
                     /gene="vanH"
                     /locus_tag="ACHATZ_003072"
     CDS             3024..3995
                     /gene="vanH"
                     /locus_tag="ACHATZ_003072"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH"
                     /protein_id="HFM8405496.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSTDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3988..5016
                     /gene="vanB"
                     /locus_tag="ACHATZ_003073"
     CDS             3988..5016
                     /gene="vanB"
                     /locus_tag="ACHATZ_003073"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8405497.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5022..5630
                     /locus_tag="ACHATZ_003074"
     CDS             5022..5630
                     /locus_tag="ACHATZ_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8405498.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1650 a         1172 c         1467 g         1503 t
ORIGIN      
        1 gcgactcgtt tcaatgcttt gatgtgggta tgcagatttt ctattttggc atttgaataa
       61 ggcaattcga gggctaaacg aatgccggat tcatagttta taaaagcttt ttttatagaa
      121 tgcagttctt ttggaacgga attgggcatc gttcttacaa gtttaaagaa agtgtccgcc
      181 tctttgtcgt gaaacgcttg aagaatttct tgatagtaaa aataggcgat tcgtaattcg
      241 tcagagatcg aaagcagacg atccaccact tcactttcgg tgagatacgc ccaaccaaaa
      301 ttgcgccatt tggtccattt gtcataaaca agcttgtcct gtcgtttgat cagaagccga
      361 gacaacgcct ttaattgtcg ataatatttg gcttgtgttg gatcgtttcg atcaaagctt
      421 ttcatgattc gtacgcgaat atcctcaaat ttacgaatga gatgtttgac aatgtgaaaa
      481 cgatccacaa cgagttttgc ctttggaaaa cattccttta tcagagaggc ataagaaaca
      541 ttcatgtccg tcacgatgac ctttaccttc aatcgtgcgc gtcgttcaaa ttgcataaaa
      601 taagaaacga gagccttttt cttccgtgaa ggaagaatat ccagtaattt tccagtatcg
      661 ccatccatac aagaaaagga gtagggaccg acttgatttt tcagtgaacg aaattcatcc
      721 attaataaac aacgcggtaa ccaatttctt tttacttttg tcatggggat cagagaagcg
      781 agtgtccgag aaacggtcga ccaagaaacg ccgcaatgct tagcaattgt cgtcattgct
      841 tgtttttcag ttagctcttc taaaatttta tagcgcactt gtttagcgat aaagcagttt
      901 ctatcgacaa tgtaggtatt cgccgtaaaa taggtgtggc agccacgaca gtggtagcgt
      961 tgtttcgcta aagcaagata agtcagttgg ttcgcacatt tattaagttg aatgagcgat
     1021 tttttctttc cgttcttaac gatcatgcct tcatgattca tctgtttaca caaaggacag
     1081 cgacgaattt ctttacttaa cgtcgcagaa acaacaagac tcttgatatt cttaatgctc
     1141 ttcgattcca catttgtgat gaaaatgttt ttatctttga ttccaagtag tagtttgata
     1201 tgatgaagat caggcataca gaacgctcct ttgattgtgt tgtggtgatt caattctaac
     1261 ggagcttctg tatgttttgt tttttcatgc tcaaaaggga ccgatagaat gattttctat
     1321 cagtcctaga aattatagag ccgattttga ccgacaggta taaccctgcc ggtctttttt
     1381 tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat
     1441 aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga
     1501 aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac aatccgtgga
     1561 aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc tgtttttggg
     1621 gtggagactg gcttccgtat tggaggcaac acagataccg cccatccctg caactcatac
     1681 aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca ccgccaaaga
     1741 acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc ccatccccgc
     1801 acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata ttcggatttc
     1861 tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc cgattgtcgc
     1921 atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa ttgccgaata
     1981 taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt gggtggccgt
     2041 gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg acggaattca
     2101 ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt ttggttttat
     2161 tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc cgtggcatta
     2221 ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt gccttgagga
     2281 atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa aagattgaca
     2341 cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg
     2401 ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat
     2461 ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga catgatatac
     2521 caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata
     2581 agaccggggg aaaccttttc tttctggttg ttcctgcata cgccattgac aattatccag
     2641 cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg
     2701 gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc
     2761 taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtt cgccgacaaa
     2821 gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc
     2881 gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata
     2941 atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt
     3001 gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt tttggatgcg
     3061 aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt atccctacgc
     3121 tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat caatgcgtta
     3181 gcgtaagcca taagtccgag gtttccgagg cgacaattct tgcgctgaga aaggtcgggg
     3241 taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact gccgccgaga
     3301 gaatgggaat ctcggttggc acggttgcgt attcgacgga cagcgttgcg gattatgctt
     3361 tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc gtggcgcaac
     3421 aagatttcag attggatcgt atccggggga aagaactggg ggatatgact gtgggagtta
     3481 ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt ggatgccatg
     3541 tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt gatgagcttc
     3601 taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc cgccatctga
     3661 tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac accgggcgcg
     3721 gagcgcttgt cgataccggg gcgctggtgg aggcgttgga aagcggaaag ctgggtggtg
     3781 cggcactgga tgtgttggag ggcgaggatc agtttgttta taccgattgc tcgcagaaag
     3841 tgcttgacca tccctttttg tcgcagcttt taaggatgcc gaatgtgatc atcacacccc
     3901 atacggcgta ctacactgag cgtgtgctgc aggatactac agaaaaaaca atcaggaatt
     3961 gtctcaattt tgaaaggagt ttacagcatg aatagaataa aagtcgcaat catcttcggc
     4021 ggttgctcgg aggaacatga tgtgtcggta aaagccgcaa tagaaattgc tgcgaacatt
     4081 gatacggaaa aattcgatcc gcactacatc ggaattacaa aaaacggtgt atggaagcta
     4141 tgcaagaagc catgtacgga atgggaagcc gacagtctcc ccgccatact ctccccggat
     4201 aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg gcgtattgat
     4261 gtggctttcc cggttttgca tggcaaatgc ggggaggatg gtgcgataca ggggctgttt
     4321 gtattgtctg gtatccccta tgtgggctgt gatattcaaa gctccgcagc ttgcatggac
     4381 aaatcactgg cctacattct tacaaaaaat gcgggcatcg ccgttcccga atttcaaatg
     4441 attgataaag gtgacaagcc ggaggcgggt gcgcttacct accctgtctt tgtgaagccg
     4501 gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga acttaacgct
     4561 gcgatagaag cggcaggaca atatgatgga aaaatcttaa ttgagcaagc gatttcgggc
     4621 tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt tgattgtcgg cgaagtggat
     4681 caaatccggc tgagccacgg tatcttccgc atccatcagg aaaacgagcc ggaaaaaggc
     4741 tcagaaaatg cgatgattac agttcccgca gacattccgg tcgaggaacg aaatcgggtg
     4801 caggaaacgg caaagaaagt atatcgggtg cttggatgca gagggcttgc ccgtgttgat
     4861 ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg tcaataccct gcccggtttt
     4921 acatcgtaca gccgctaccc acgtatgatg gccgccgcag gaatcacgct tcctgcactg
     4981 attgacagcc tgattacatt ggcgttaaag aggtgataag tatggaaaat ggttttttgt
     5041 ttttagatga aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca tgggataact
     5101 tcacaggaaa accggtggat gggtatgagg tgaatcgcat catcggcaca aaggccgtgg
     5161 cgtttgctct gcgcgaggca caaatccatg cggctgcgct tggctatggc ttgcttttat
     5221 gggatggata tcggccaaga actgcggtgg actgcttcct gcgttgggca gcgcaaccgg
     5281 aggacaatct cacaaaagaa aaattttacc ccaatataga gcgagccgag ttgattacaa
     5341 agggttatgt ggcctcacaa tccagccata gccgtggaag cgcaattgat cttacgctct
     5401 accacctgga tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt atggacgaac
     5461 ggtcgcatca tacagcaaaa gggatagggg atgcagaggc acaaaatcga agatgcttgc
     5521 gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg cactataagt
     5581 tgattgatga gccatacccc gatacctatt ttaattttgc cgtttcataa tgaaagtatt
     5641 ggttttgtat cagattttgg atttgataaa atctatgctg gtcatggaca atcttttact
     5701 ggaaagtggt taaagcatct tttgtaatac attgcaaatt atagtattca atctgcccag
     5761 cggcagaaaa gaaaaaaacc gctgttgggc ag
//
LOCUS       DAYYAJ010000295         5643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.158, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000295 DAYYAJ010000000
VERSION     DAYYAJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.158"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..887
                     /locus_tag="ACHATZ_003075"
     CDS             <1..887
                     /locus_tag="ACHATZ_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8405499.1"
                     /translation="DVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQLANY
                     QLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEIYHFD
                     FLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKRGVTT
                     NYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVT
                     DNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKRFFTT
                     DSAIYEQYEYDLSSVKNK"
     gene            complement(1030..1971)
                     /locus_tag="ACHATZ_003076"
     CDS             complement(1030..1971)
                     /locus_tag="ACHATZ_003076"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8405500.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2097..3218
                     /locus_tag="ACHATZ_003077"
     CDS             2097..3218
                     /locus_tag="ACHATZ_003077"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8405501.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3476..4978
                     /gene="eat(A)"
                     /locus_tag="ACHATZ_003078"
     CDS             3476..4978
                     /gene="eat(A)"
                     /locus_tag="ACHATZ_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8405502.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5045..5572
                     /locus_tag="ACHATZ_003079"
     CDS             5045..5572
                     /locus_tag="ACHATZ_003079"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8405503.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
BASE COUNT         1832 a         1012 c         1199 g         1600 t
ORIGIN      
        1 acgacgtatt gctaattgcg aaaaataaat cactttattt ttttccttcc atccgctctt
       61 tagatgtttg gtccggaaaa gatttactac agtcggaatt cattaaatca gctcgacaac
      121 agttggcaaa ttatcagctg atcgtttttt tagattatgg atttgatatg gagatggctc
      181 gacgcgtgcg tccatttacc aatgcaaaaa tcgtgttatt tttttggaac cactttaaag
      241 cagaacacga ggcgatgctg aagcaggcac aagatgaacc ggcaatcgat gagatttatc
      301 attttgattt tttagaagcg aaagaacttg gactaaaaca taatagttcc ttttattcga
      361 aacatatgga tttttcttct agcgaaccaa agacggatct attttttggt gcaacaaata
      421 acggcagaaa agaacgggca gaaaaatata agcaggaatt tacaaaaaga ggtgttacga
      481 ccaattactt tattcttccg cttcgaggaa atgaacaacc tggatacctt acttatgaag
      541 aatatctgaa aaaaaccggg gaatcgaggg ggattctaga actgttgcgg gaagggcagc
      601 aaggagtgac tttgcgaacg tttgaatcta tgtattttca aaagaaacta gtgacagata
      661 atcaagccat agctcattat cagttttatc attctgaaaa tatttttttg cttcaagaac
      721 gtgatttaga tgaattaccg gaatttttgc atacgccatt tcaaccaatc ccatcagaac
      781 tgttggaatt ttttgatgca gaaaattggg caaaacgttt tttcactact gactccgcta
      841 tttatgaaca atatgaatac gatctttcct ctgttaaaaa taaataaaag tgccaaccaa
      901 tcatagatga aaataaacaa gcgcaattct tatggcaaca tgagaattgt gtttttcttt
      961 ttgaactttc atgggggtaa aaaaatgaga accagctgct gatccagctg attctcatca
     1021 gagagccttt tattctgcgc caaggattcc ttcttttgca tggactaatt gggtcaaaaa
     1081 ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt cgccaaacgg caccattgat
     1141 gtagtcgatt tcagtcaaac gatggttttt gatcaggtct tggtacatag atgggtaatg
     1201 caacccgatg ttgtcaggat cataagtaga agcaatatgt tggtacactt cttcttgatc
     1261 taatgtgatt ccttcttttg ctgcgataga agcaaactca gatacaatcg ttcgaaccat
     1321 gccctctgcc gctttttgcg cacctaattc agcaatatta caatccaata tcgtgcataa
     1381 tccatttaat gttccgttta cacaagcttt tcgccaaatg gaataacgaa cattatgact
     1441 atagattgga ttcaattttg cttcttgaaa gacttctaca actttttttg taaatgcttc
     1501 gccttctggt tccaagttct ctagttcgat ttctccatca cctaataatg tgacttttcc
     1561 gggaccagct agaccagctg tccacatcgt gatcccaaat aagatatttt tctttggtac
     1621 atatttttcc agtacatctt catgacctaa tccattcagt aaacacaaaa cataagtatt
     1681 ctcagtaata atggattgga tcgaacgaaa catatcatct aattgattcg cttttgtcaa
     1741 ggctacgatc aaatccacgt gtttgttgct ttcgatgatt tcttctggtg aatagattgg
     1801 cagttttgca actacttctt tgccattaaa gtcagcaatc aatccatttg tacgaatggc
     1861 ttcaatgtga gcaggccagc gatcgatcaa gacgacctca tttccgcttt gatgcaacat
     1921 taatccgatt cggctgccca ttgctccggc tccagctatt gcgattttca tgttttttct
     1981 ctcctttatt caatgagttc ccttttagta tatagaaaaa cagaagaaaa tagcattgtt
     2041 ttgtttgaaa aaagtgaaaa aaagtgtctt acttaagaag aaagtgaggg atatagatgg
     2101 atattttggc ggcaatcgat tcatttaaag gaagtgcttc ttcatttgaa ttaaatcagg
     2161 cagttctaaa tggttgggaa gaccgtaaag gaagaaaaat caatgtgccg attgcagatg
     2221 gcggagaaaa tactgcagaa gcaatctatt acgcgctcgg gggaacctgg cgtacgattg
     2281 atgggatgga tctcttattt cgtcagcgaa aaatcagtta tttattgacg aactggcaag
     2341 gaaaaaatac cgcagtcatt gaatctgcac aagtattagg attagattta cttgataaac
     2401 caacggatca aaccatccga cttgcttcta gttatgcctt gggagagctt atcaaagata
     2461 cactgcttca agaagtggaa caaatcattg tcactttagg aggaagcgga tgtagcgatg
     2521 gtggtctagg attactccaa tctttgggtg cttctttaga agggtacaca gaaggaaatc
     2581 ctttgttatc tactagaaaa cttcatttgg atcgaaggaa cgttcttgtt tctaaagtaa
     2641 aagtgatcat cgctgcagac gtgaccaacc catatacagg tcaacaagga gctgcttggc
     2701 aatttggaag acaaaaagga ggaacggata cgacgttagc tttcttggat aaacaagctg
     2761 aaaagattgc tcagcagata aaagatgttt gggaggtgga tttagatgac atcccaggaa
     2821 gcggagcagc aggcggtatt ggcggagccc tttttttgtt aggagcgaag atggtctctg
     2881 gttttgagtt ggtcagccaa atcactggat tagaaaaaca gatcgaacaa gcggatgtca
     2941 tcattacagg agaagggcgg attgatcagc aaacaatcca cggaaaagtt ccatatggcg
     3001 tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga agtagagacc
     3061 gagagctgga aggactcttt gattttctcc ccattgtctt aagcattcaa ttaggaccaa
     3121 tcgatttaga ggaagcaatg gaacacaggc aaacattaga aaaaacagaa atcttaggac
     3181 agacactcag cagatttttc aacattctcc cgaagtagtt gacaaaaaca atttctttat
     3241 gctatcgtaa tttcaaatat aggacaaagt gagggatccg tgcattgaaa aattaagtcg
     3301 attgatgaaa gctgcattca gactgtacct tgtggagaca agggagcagt gtgcagattt
     3361 tcgggattgg cttggttctt tcgatgtatg ggtctttttg tgtgccccaa cccttgcatc
     3421 ctctttagcc agttctgaaa ataggactgg tcttttttta cgaggtgatg gaataatgtc
     3481 taaaatcgaa ataaaaaatc tgacattcgg ctatgacagc caaggcacat tattatttga
     3541 acaagccaat ctaaactttg acacacaatg gaaactagga cttatcggac gaaacggtcg
     3601 aggaaagaca actttactga atatcctgca aaacaaactc ccttatcaag ggcaagtgat
     3661 ccatcagcaa gcgtttgtct atttcccgca gcaaacaaaa gataaagagc gtttgaccta
     3721 ttatgtgtta aatgagatta tggattttga gatatgggaa attgaaagag agctccaact
     3781 gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcag gaggagagaa
     3841 gacaaaagtc ctactggcac tcttatttgt ggatgacact catttcccgt taatcgatga
     3901 accaacgaat catttggata tctctggcag aaaacaagta gcggcttact tgaaaaagaa
     3961 aaaacaagga ttcatcgtgg tgagtcatga tcggggattt atcgatgaag tagtggacca
     4021 tgttttagca atcgaaaaga gccaactgga actttatcaa gggaatttct ctatctatga
     4081 agaacagaaa aaacttcgtg atgaatttga aatagctaaa aatgaaaaat tgaaaaaaga
     4141 agtcagcagg ctaaagaaaa cagcagctga aaaagcagaa tggtctcgtt cccgagaagg
     4201 ggacaaaaca aagaaaaaag tcggattcat cgatacggaa tctaggcgag tgaataaagg
     4261 agcagtaggc gctgatgctg cacggacgat gaaacgatct aaagcaatcg tgaatcggat
     4321 ggaaacccag atcagtgaga aagaaaaatt attaaaagat atcgagtata tcgatgcttt
     4381 gacgatgaat agccaagtgt ctcatcataa acggctgtta agcgtagaaa atcttcaatt
     4441 agggtatgaa aatctgttat tcgagccagt tcattttacg atcgaacctc atcagcggat
     4501 ggcaatttca ggtcctaacg gcgcgggaaa gtcatccatt atccattatc ttctggggac
     4561 attcaacggc aaggttgtag gagaaaaatc ccagcccaaa catttgacca ttagttacgt
     4621 acgccaaaat tatgaagaca atcgagggac attggcggaa ttcgcgaaga aaaaccgagt
     4681 agactatcaa gcatttttga ataatctccg aaagcttggg atggaaagag atgtttttca
     4741 taataaaatc gaacagatga gtatgggaca acggaaaaaa gtggaattgg ctaaatcttt
     4801 atcccaacca gctgagctct atatatggga tgaaccattg aattatttgg atgtcttcaa
     4861 tcaagaacaa ttagaacaac tgattttgaa cgtgaaacct gctatgttac tagtggaaca
     4921 tgatcaaacc tttctagata aagtatctac tgagattatt tctcttgaga aaatctaaac
     4981 cttgtaatct caaagacaac aaggtatggt taaggagaac tttgtcaacg aaggagagaa
     5041 tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag gattagtttg
     5101 cgggcgagca gatgtgccgc tgactgaaaa aggacagatg caggcacagg cactagcaga
     5161 aaaggtagta gatttgcctg ttccaatcac gaaaatcatc cattcccctc ttcaacgagc
     5221 aagagataca gcacaagcag tagttgatcg tctcaatttg ccgatgaccg ctgatgagcg
     5281 attggtagaa atggattttg gggattatga cggactgcca agcaaagatg aaaactttca
     5341 aaaagcgcgt ttagcgtttg ctgtacgttt cccaaatgga gagtcggtct tagatgtgta
     5401 cgcacggatc gttcctttat tgaaagaatg catggaagac gaagaaaatg tctacttgtt
     5461 agtctgtcat aatgccttga taagagtgat caatgcttat tttcatccaa tgcctaatga
     5521 agggttcttt acattcatgg tagacaatac agaattaatt tcttatgagt aattagactt
     5581 ttgtgttatt taaataaaat gtttcaaggc actgcttttg cttagcagtg ccttttttgt
     5641 ggc
//
LOCUS       DAYYAJ010000296         4128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.178, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000296 DAYYAJ010000000
VERSION     DAYYAJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.178"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..617)
                     /locus_tag="ACHATZ_003080"
                     /pseudo
     CDS             complement(247..617)
                     /locus_tag="ACHATZ_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            762..947
                     /locus_tag="ACHATZ_003081"
     CDS             762..947
                     /locus_tag="ACHATZ_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8405504.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1105..1272
                     /locus_tag="ACHATZ_003082"
     CDS             1105..1272
                     /locus_tag="ACHATZ_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405505.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1890..2549
                     /gene="vanR-B"
                     /locus_tag="ACHATZ_003083"
     CDS             1890..2549
                     /gene="vanR-B"
                     /locus_tag="ACHATZ_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8405506.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2552..3913
                     /gene="vanS-B"
                     /locus_tag="ACHATZ_003084"
     CDS             2552..3913
                     /gene="vanS-B"
                     /locus_tag="ACHATZ_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8405507.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(4004..>4128)
                     /locus_tag="ACHATZ_003085"
     CDS             complement(4004..>4128)
                     /locus_tag="ACHATZ_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8405508.1"
                     /translation="NLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1182 a          844 c         1017 g         1085 t
ORIGIN      
        1 ctttccattc aggggtttgg ggatttcccc aacaagcaaa acggacgcgg cccggcaggg
       61 acggggcagg ccgtttttcc gggagtggct acactcccta tgcttgctac gttactgttt
      121 ctccactaac aagacgtttc caaagcccca aactacccgc aaaatagccc tctggaaaga
      181 aaaaagtgca aaaaaataga agctacgctg tgcagctccc gctaaaagta aaacagagga
      241 tgccagttac acatcctccg tttcattctt cttcggcttt tcaattagta tggcgctgtc
      301 aatcgcgccc tcgatgatcg gcaggtattc ttcggggcag agtttcagct tgtggctgac
      361 cctttgccgc agttcgcttt cctcccgcat gatctccggg ttaaagtagc gttccacagg
      421 aagcccgcag gctttaataa gctgtatgac aaccggcaga ctgggaatgg cccgtcgttt
      481 tcgatgttgg caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa
      541 acgcgctttg cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa
      601 tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt
      661 ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc
      721 ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga
      781 aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg
      841 gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa
      901 agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg
      961 gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact
     1021 ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga
     1081 aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa
     1141 aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa
     1201 aaggccggca tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta
     1261 atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga
     1321 aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga
     1381 tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga
     1441 ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc
     1501 tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag
     1561 gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag
     1621 aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa
     1681 ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta
     1741 attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt atcccaatga
     1801 cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta
     1861 tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg
     1921 atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg
     1981 cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat caactggtta
     2041 ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga
     2101 aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg
     2161 cgtttgatgc agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa
     2221 agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca
     2281 ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga
     2341 cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgc
     2401 atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc agcacagtcc
     2461 acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg
     2521 gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt
     2581 tttttcctat acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc
     2641 acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca
     2701 gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct
     2761 gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact
     2821 ctatgccaca ccaaatgcca atacatcaaa tagttttaga cccaactttc tttatgtggt
     2881 acatagagat gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta
     2941 tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt
     3001 atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc
     3061 gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg
     3121 cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct
     3181 ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc
     3241 agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct
     3301 tgaaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat
     3361 ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag
     3421 aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga
     3481 ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca
     3541 aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc
     3601 agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa
     3661 atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct
     3721 gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcagaaa
     3781 aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
     3841 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
     3901 atcaacattg taaatattta aaacttaaat gattttgggc tctttgtcaa ataggactga
     3961 tagagtgaga taagaacatc taaggtagaa ttccttttct actttagatg ttttttgatt
     4021 cataggtaat cagattattt agtaagaaaa ttctggtctt cattttccga aagcttcgaa
     4081 agccataagc gactcgtttc aatgctttga tgtgggtatg cagatttt
//
LOCUS       DAYYAJ010000297         4128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.179, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000297 DAYYAJ010000000
VERSION     DAYYAJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.179"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1556
                     /gene="msr(C)"
                     /locus_tag="ACHATZ_003086"
     CDS             78..1556
                     /gene="msr(C)"
                     /locus_tag="ACHATZ_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8405509.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1603..2049
                     /locus_tag="ACHATZ_003087"
     CDS             1603..2049
                     /locus_tag="ACHATZ_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8405510.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2248..2763)
                     /locus_tag="ACHATZ_003088"
     CDS             complement(2248..2763)
                     /locus_tag="ACHATZ_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8405511.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2880..4082
                     /locus_tag="ACHATZ_003089"
     CDS             2880..4082
                     /locus_tag="ACHATZ_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8405512.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1227 a          703 c          895 g         1303 t
ORIGIN      
        1 ttttttgcaa acagattttt ctgctctttt ttagtatcga taattgttgt tttacatgaa
       61 ataggagtgt aaaacatatg gaaaatttag cagtaaatat aacaaatcta caagtcagct
      121 ttggcaacca gctagaacta tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag
      181 ggatcatagg agaaaatggt gtcggtaaat cgactttgct caaactaata gccggtgaac
      241 tttttcccga tcatgggaaa atccaaacag agatcacctt caactacctg cctcaattaa
      301 cctatctcgc tgaagcaaag gacctaaatt tggaattagc cagtcatttc cagttaagac
      361 tggaagaaac ttcagagcgg aaatggagcg gaggggaaga acgaaagatc gagttgatac
      421 gtcttctttc ttcttatgaa caagggatgc ttctagacga gccaacaacc catctagata
      481 gaaaaagtat tgatcgactg attgaagagc ttcgttatta ttatggtacg ctggtttttg
      541 ttagtcatga tcgctatttt ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg
      601 gagaaatccg agagttttcg gggaattata gtgcctatct cactcaaaag gaattggaga
      661 aaaagactca gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat
      721 cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa
      781 agcaacaaat cagactggat cggttgtctt cctctaaaca aaaagacagt gtacaaaaag
      841 ccatccaaaa gaatgcgaaa acattagaga gaagactcca aaaaatagga gaaacaacca
      901 agccgcagca gatgaaacaa atccgttttc cagtaccaaa atctcttgaa ctccacagcc
      961 gctatcccat catgggacaa aatgtccaat tggaaagaag tgggagaaca ttactggtaa
     1021 atggtgattt tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag
     1081 gtaagacaac cttattagaa catatccgca aacaaggaga aggaatcctt ctctctccga
     1141 aagtaagctt tcaagtatat cagcaaaagg gttatcaaat gacatctgaa gaatccatca
     1201 ttcgttttgt catgagacaa acagagtttt cagaatcgct tgtccgtagt ttgctgaatc
     1261 acttagggtt tgctcaggaa actttgacga aaccgttatg tacattaagt gggggagaag
     1321 cgacccgtct gacgattgct ttgcttttta ctaagccaag taatgtgttg ctgttagatg
     1381 aaccgactaa ttttattgat atggcaacga tcgaagcttt agagaagcta atgcaaatat
     1441 atccgggaac gattttgttt acttctcatg attcttactt tgtcgagcgt acggctgatg
     1501 aagtttatga aataaaaggg cagaaaataa aaaaagtact tacgagaaat ttttaaatgt
     1561 gctacgatga ttttcgtgac aaatttaaag gagtggtaca gattgcctac aaataaaaaa
     1621 gaaggaataa tttttacaac aatgatgtgt tttttgatgg tcttggggat gagcgcgtat
     1681 aatttatggc tgcatcaaga tcttcattta tcaagtttgt tgattggtct agtgcctgga
     1741 ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc
     1801 tttaaactac cagtaaatcc agaaagcaag gtccaaatga tcttaactat ttcatgcttg
     1861 atggttttag gaatggtgac atgtatgtct ttattcggtg tgatcatgga acaaggaata
     1921 cctgatcaat tattctctgc ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta
     1981 ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt
     2041 aatgaatgat ggaacactgt tatttgaaaa aaggccttgg catgcttttt gtcatagtct
     2101 cttgaatgca gttctagaaa aatagtgatc cgaacgatag gttttggcct gtcgttcgga
     2161 tcgctttttc ttttccaact gtattgctct tttattgaag ataatttgct cttcaataga
     2221 tatttctaga aaggttctgt ttatccctta ctcaaaaaac tgctgcattt gttctcttac
     2281 ttgctgacac cattggagtt cctgctgaca aagcataatt ctgcgctgga tcacaacata
     2341 acgtccaaat tcttgtttct tgtctagcga gaggtcattc aatttttttt gatttttttg
     2401 catgattttg ttcagctgtt gttctctagt gaatagcagt ccagtagctg ttgttttatc
     2461 gagcgtcgaa aaagcataga tttttgctag aaattcatct ttttgcgtag gagcgggagt
     2521 ttcctctttg atccattcct ctacaagagc acgtccttca tcagtcaagt ggtagatctt
     2581 tttttgagag tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt ctaacttggc
     2641 aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa
     2701 ttcataccca gaacgtggtt tggataaaag cacgcataat aatatatagg ctaaagtatt
     2761 cataaaaatc ctacctctcg tcttattttc tttattatat cacagtaaag tttccacgtt
     2821 ttacatgtgc atattgacat gtaaaataaa tcatgtatga tgctaataga gagaaaggga
     2881 tggaaatatt ggagaatcaa atagatatac aagaacaaac aaatgaagaa acaaagggga
     2941 aaagttcagc gctagttgta gcagctacct gtatgatcgc ttttatggga gtgggtctag
     3001 tagacccaat cttaaaaacg attgctgcac agttaaacgc tacgccagct gaaacgaccc
     3061 tgttatttac tagttacatg ttagtcactg gtgtggtgat gctatttaca ggtttcttat
     3121 ctagtcggat cggtttgaaa aaaacattga tgacaggatt gtttatcatc gttgtattcg
     3181 ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg
     3241 gtgtcgggaa tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg
     3301 gtaacacaga aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg
     3361 gccctctagt cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag
     3421 catttttgat gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac
     3481 caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt
     3541 cagttggact aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc
     3601 catttttatt gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg
     3661 tgcttctggc tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt
     3721 atcggacaat tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg
     3781 gaacttctat gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa
     3841 ttatcaatac actgttgacg acgattgcta tggaaatacc aggtctgcaa cgaaacgttg
     3901 cttcgtccag ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag
     3961 gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc
     4021 ttatcagtgt atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat
     4081 aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DAYYAJ010000298         4024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000298 DAYYAJ010000000
VERSION     DAYYAJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.184"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..266
                     /locus_tag="ACHATZ_003090"
     CDS             <1..266
                     /locus_tag="ACHATZ_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8405513.1"
                     /translation="DEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRFRIGYN
                     RAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            496..1563
                     /gene="rlmN"
                     /locus_tag="ACHATZ_003091"
     CDS             496..1563
                     /gene="rlmN"
                     /locus_tag="ACHATZ_003091"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8405514.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            1553..1759
                     /locus_tag="ACHATZ_003092"
     CDS             1553..1759
                     /locus_tag="ACHATZ_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405515.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1756..2859)
                     /locus_tag="ACHATZ_003093"
     CDS             complement(1756..2859)
                     /locus_tag="ACHATZ_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8405516.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3411..3959
                     /gene="aac(6')"
                     /locus_tag="ACHATZ_003094"
     CDS             3411..3959
                     /gene="aac(6')"
                     /locus_tag="ACHATZ_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8405517.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1331 a          685 c          824 g         1184 t
ORIGIN      
        1 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
       61 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
      121 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
      181 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
      241 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
      301 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
      361 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
      421 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
      481 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
      541 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
      601 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
      661 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
      721 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
      781 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
      841 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
      901 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
      961 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     1021 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     1081 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     1141 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     1201 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     1261 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     1321 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     1381 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     1441 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     1501 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     1561 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     1621 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     1681 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     1741 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     1801 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     1861 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     1921 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     1981 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     2041 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     2101 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     2161 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     2221 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     2281 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     2341 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     2401 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     2461 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     2521 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
     2581 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
     2641 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
     2701 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
     2761 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
     2821 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
     2881 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
     2941 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
     3001 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
     3061 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
     3121 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
     3181 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
     3241 cctgatcttt cctattttga ataaagtgtt tctgtataat tttttatttt ataagtaaga
     3301 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
     3361 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
     3421 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
     3481 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
     3541 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
     3601 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
     3661 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
     3721 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
     3781 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
     3841 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
     3901 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
     3961 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
     4021 actt
//
LOCUS       DAYYAJ010000299         3507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.194, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000299 DAYYAJ010000000
VERSION     DAYYAJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.194"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..766
                     /gene="erm(B)"
                     /locus_tag="ACHATZ_003095"
     CDS             29..766
                     /gene="erm(B)"
                     /locus_tag="ACHATZ_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8405518.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1029..1892)
                     /locus_tag="ACHATZ_003096"
     CDS             complement(1029..1892)
                     /locus_tag="ACHATZ_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8405519.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(1894..2166)
                     /locus_tag="ACHATZ_003097"
     CDS             complement(1894..2166)
                     /locus_tag="ACHATZ_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8405520.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2183..2392)
                     /locus_tag="ACHATZ_003098"
     CDS             complement(2183..2392)
                     /locus_tag="ACHATZ_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8405521.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2470..3366)
                     /locus_tag="ACHATZ_003099"
     CDS             complement(2470..3366)
                     /locus_tag="ACHATZ_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8405522.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1059 a          627 c          544 g         1277 t
ORIGIN      
        1 tagatgtaat cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt
       61 tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga
      121 taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag
      181 taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt
      241 aaaactgaac actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa
      301 caaacagagg tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa
      361 aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta
      421 caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat
      481 tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt
      541 cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac
      601 gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt
      661 tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt
      721 attgtctatt tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct
      781 tttgtaaatt tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag
      841 ggcacctcta ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa
      901 atttttaaat ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg
      961 aggaaatgtt tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt
     1021 tttaaggttt aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc
     1081 aagctttcca ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga
     1141 accatttttt gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga
     1201 tttaattctc tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag
     1261 agtttcactc cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg
     1321 gtttctaaat tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc
     1381 ctagctgtca ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat
     1441 gattcaattt tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc
     1501 attgtggctg tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta
     1561 tagccttgat cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta
     1621 acgtgtttta ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt
     1681 tgtttaaagg tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct
     1741 gatcgcaaac tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat
     1801 tcaaccgcta gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat
     1861 tgcttttcgg tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat
     1921 ttgttttgaa tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa
     1981 tggttgttga aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc
     2041 tagtaaacga gtatttttag tatctagttc atgattcaaa acataattta atacccgatt
     2101 ataaacactt gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac
     2161 tgccatttca ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt
     2221 ctaaggcttg atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta
     2281 tgatatggtg caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg
     2341 cgctgattgg cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc
     2401 acttctttct atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga
     2461 tttgtgattc tattcttttt cattttctaa ttgaatgatt cgctcaatca tttcaaaaaa
     2521 tacgttctca tacatagaca aaactttttt gtcatagcct ttgtgttctg taatgccatt
     2581 tttagaccaa gtacttactt tattacttcg cttgataata tttcggaaaa ccaaattatc
     2641 ctctttatgt tctttgtaca gttcttccag gtttgatttt atcgttgcgc tgtccgtatc
     2701 aactaaataa ggaacaaaac caatcatatc tagtccaggg ttaaactgtt cttgcaaatc
     2761 aatcaaatag gaaatatagt tttgaatgtt gtttgtactt tcttcttctg cttgtaaagg
     2821 gatcataacg taatcactcg ccacgattgc attatttgta taaacgcttg gcgttggtac
     2881 agtatcaata ataataagat catagtcact ttttaaaggt gctaaaagag tagcaagcaa
     2941 tctactttca ttttcaaacg tccatgagcg agttaatttt ggcagtaaca tcaaatcaaa
     3001 cgtgccaggg atcaagtcta aattatcagt caaatgaata atagaagaag ccaagtttcc
     3061 atttttcaag ccttcataaa aattgacacg tggcaattct acctcaaaag tttttgctaa
     3121 gtcttttgtc aatgttgcct gtaagtcctt atcgatcatt aaaactttta aattcaattt
     3181 gtctgtcaag taagcaaaca tagtcgataa tttggacttt ccaacaccac ctttaaagta
     3241 attattcaag ataataattg cttcattttt attgtttaaa atacgtctta attcttcaag
     3301 tatttttagt ttttccttct ccataactac gccccattct ttttttgtat agtaatattg
     3361 tatcattaag aatcacaaat cacaagtgat ttgtgattaa tcacttgtga tttgtgataa
     3421 gtgatttgtg attaatatat aaaagccctc tttaaagggc ttttataggt tctgttgcaa
     3481 agttttaaat aaagaataaa atccctt
//
LOCUS       DAYYAJ010000300         2569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.208, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000300 DAYYAJ010000000
VERSION     DAYYAJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.208"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..637
                     /gene="dfrG"
                     /locus_tag="ACHATZ_003100"
     CDS             140..637
                     /gene="dfrG"
                     /locus_tag="ACHATZ_003100"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8405523.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(709..>2569)
                     /locus_tag="ACHATZ_003101"
     CDS             complement(709..>2569)
                     /locus_tag="ACHATZ_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8405524.1"
                     /translation="QYDLLTPKRFATRYNKMLFLPTSFKWNGSEYPIQYNYCINPFCC
                     NFGKEQHKFKDVKGKPSRYKMTGSSKDKGHKGMYCNDNPIGRGVSQNCTVTPLSNWSV
                     VEEIKRLIEINSIQDVEPDYQFHKEGCSEEESTPFNEPKQFYKRGKSRGKSQRYQCKA
                     CKKFTNVLPKREETTTYHQQKNTILPMFAKMVVGRVSVSRTCDILGIGVGTYYHKLEW
                     LYRRCLEFLERYETQPLQTKKFNEMWLNTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQ
                     TYIVVSAEVLSRYVFRSDVAYDWNISMDELNEDTRKFKEDHLNTFSRKNDRLDWSYYP
                     QEPSANDSENRNAYLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMI
                     SDDDFSIRNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIR
                     GFKTKFLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSV
                     DCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYAN
                     FNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          793 a          427 c          460 g          889 t
ORIGIN      
        1 tttgtgattt tgtggtatta tgataatata acttatagaa aaaattgctt attcaactaa
       61 cgggcaggat aatgagaaaa ggttttttga tagaaataag aaattagatg gtaaattagt
      121 taaaaaggct gtgttataaa tgaaagtttc tttgattgct gcgatggata agaatagagt
      181 gattggcaaa gagaatgaca ttccttggag gattcccaag gactgggaat atgttaaaaa
      241 tactacaaag ggacatccga taatattagg taggaagaac cttgaatcaa tcggaagagc
      301 cttacctgac agaagaaata ttattctgac gagagataag gggtttacct ttaatggttg
      361 tgaaattgtt cattcaatag aagatgtttt tgagttatgt aaaaacgaag aagaaatttt
      421 tattttcgga ggagaacaga tttataattt gtttttccct tatgttgaga aaatgtacat
      481 cacaaaaata catcatgaat tcgaaggaga tacttttttt ccagaagtga attatgagga
      541 atggaatgag gtatttgccc aaaaagggat aaagaatgat aaaaatccgt ataactacta
      601 ttttcatgta tatgaaagaa aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa
      661 cagtaaagat aaagcacaag agcttgggat tactccaagc tctttttgtc atttgaaata
      721 aattatatct ttccaatcaa attgtttatc tgtaatccct aatctttgag caggtgtcaa
      781 aaccttttta tccgctgatt taaaagggcg acaaaagttg tagtaagttc ttagaattgt
      841 aatcgcaaat tgagcatact tcggattgaa gttagcgtaa atatagctct tcttatcacc
      901 acgagctgtt gttaatggtc tttcaagaga tgatatataa cgtcttatct gttgaataaa
      961 gctattggtc gaatggtcat taacatttaa tagcatcttt gcgatttcgt ttggttccag
     1021 agcagataaa tccgttctac aatcaactga ataaaatcct ttatccttag tggctaatgg
     1081 atgtttgata gggttatcgg catatttgcg atatctttct ccatctttac taatagcctc
     1141 ttcgtggaat tgatgacttg ttagtaattc agttagataa tgagatgcca aagtccgtag
     1201 aaactttgtt ttgaatcctc tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc
     1261 gaattctttt aagcattgtt ttctgctttt cgtcttgtta acttggcaaa taaaatgatg
     1321 tgcatcgctc aaccttaatt ctttcgtgaa tactctataa aaggcgtttc taatagagaa
     1381 gtcatcatca ctaatcattc gccattcgtc ggcattaacc atttgtttaa ttaaccaata
     1441 gtgagcagtt gtagtataag gagcatcaac atttaaacca tcgacaaatc tactcctatt
     1501 agtaattttc cctaattcgt gaagataagc attcctattt tcgctatcgt tagcggatgg
     1561 ttcttgtgga taataagacc aatctaacct atcattttta cgactaaaag tattgagatg
     1621 gtcttctttg aattttcttg tatcttcatt taattcatcc attgaaatat tccaatcata
     1681 ggcaacatca gaacggaaca catatcttga taagacctca gcagatacaa ctatatacgt
     1741 ttgcatatta aggtcttcaa accccgtata tttctttgaa ccttgtcctt tcttacggac
     1801 gttatttagg tagtagtgca tcttgtccgt attaagccac atctcgttaa atttctttgt
     1861 ttgaaggggt tgtgtctcat atcgttctaa aaactccaaa caacgcctat acagccactc
     1921 cagcttatga tagtaggttc caactccaat tcctaaaata tcacaagtac gactgactga
     1981 aacccttcca acaaccatct tagcgaacat tggaagaatg gtgttctttt gttggtgata
     2041 agtagtggtt tcttctcttt ttggtagaac attagtgaac ttcttacacg ccttacattg
     2101 atagcgttga gatttgcccc tacttttccc ccttttgtaa aattgcttcg gctcattgaa
     2161 tggggttgat tcttcttccg agcagccttc tttatgaaat tgataatctg gctcaacatc
     2221 ttggatgcta ttgatttcta taaggcgttt aatttcttca acaacagacc aattagataa
     2281 aggggttact gtacagtttt gcgatacacc tctgccaata ggattgtcat tgcagtacat
     2341 acctttatga cctttatctt tggaactacc agtcatttta tatcgacttg gtttcccttt
     2401 tacatctttg aatttgtgct gttctttccc aaaattacaa caaaacggat tgatgcagta
     2461 attatattga atgggatatt cagaaccgtt ccatttaaaa gacgttggca agaacaacat
     2521 tttgttatac cttgttgcaa atcgttttgg agttaataaa tcatattgt
//
LOCUS       DAYYAJ010000301         2121 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6601
            SAMEA115708473-rid22592923.guided.215, whole genome shotgun
            sequence.
ACCESSION   DAYYAJ010000301 DAYYAJ010000000
VERSION     DAYYAJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708473
            Sequence Read Archive: ERR13198196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290285.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290285.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:23:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 24 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708473-rid22592923.guided.215"
                     /isolate="VRE6601"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACHATZ_003102"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACHATZ_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACHATZ_003103"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACHATZ_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACHATZ_003104"
     CDS             complement(1055..1345)
                     /locus_tag="ACHATZ_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405525.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1747..1983
                     /locus_tag="ACHATZ_003105"
     CDS             1747..1983
                     /locus_tag="ACHATZ_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8405526.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          723 a          364 c          336 g          698 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt
     1681 gtaggaatgc aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat
     1741 tttcctatgc aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata
     1801 tcagagcaag taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc
     1861 gaagcagtgg atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt
     1921 gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt
     1981 tgatagacaa tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt
     2041 aaaattccga tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact
     2101 ccaaaacgat ttgcaacaag g
//
